Microbiological detection pretreatment device

Abstract

The utility model relates to the technical field of microbiological detection, in particular to a microbiological detection pretreatment device which comprises a pretreatment box, a first treatment cavity and a second treatment cavity are formed in the pretreatment box, cabinet doors are hinged to the first treatment cavity and the second treatment cavity, and sample treatment assemblies are arranged in the first treatment cavity and the second treatment cavity. The sample treatment assembly comprises a sample treatment box and a reagent treatment box, the sample treatment box and the reagent treatment box are arranged in the first treatment cavity and the second treatment cavity respectively, and a material conveying assembly and a mixing assembly are further installed in the pretreatment box. A crushing rod and a stirring rod in the device respectively realize crushing of a sample and mixing of a reagent through rotation of a driving motor, the efficient rotation of the crushing rod can quickly crush the sample, subsequent detection and analysis are facilitated, and the rotation of the stirring rod ensures uniform mixing of the detection reagent in the reagent treatment box, so that the detection accuracy is improved. And the layering or precipitation phenomenon of the reagent is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to microorganism detection technical field, concretely is a kind of microorganism detection pretreatment device. BACKGROUND

[0002] Microorganism is ubiquitous, mainly exists in soil, water, air and our body, microorganism plays huge role in biological field and medical field, in medical field, by detecting microorganism, it can help doctor to determine whether patient is infected with certain pathogen, to carry out accurate disease diagnosis, in food industry, by detecting the microorganism content and kind in food, potential food safety problem can be found in time, avoid food poisoning event occurrence;

[0003] But before detecting microorganism, it also needs to be complexly handled, currently, the handling work of early stage is mostly manually, it is time-consuming and laborious to operate, and sample is easily polluted, and last detection result is prone to error. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a kind of microorganism detection pretreatment device to solve the problems presented in the above background.

[0005] To achieve the above object, the utility model provides the following technical scheme:

[0006] A kind of microorganism detection pretreatment device, including pretreatment box, first processing cavity and second processing cavity are set up on the pretreatment box, cabinet door is hingedly connected on the first processing cavity and second processing cavity, sample processing assembly is arranged in the first processing cavity and second processing cavity, the sample processing assembly includes sample processing box and reagent processing box, the sample processing box and reagent processing box are arranged in the first processing cavity and second processing cavity respectively, feed assembly and mixing assembly are also installed in the pretreatment box, the feed end of feed assembly is connected with the bottom of sample processing box, the feed end of mixing assembly is connected with reagent processing box, and the discharge end of feed assembly is connected with mixing assembly;

[0007] The top of the pretreatment box is also installed with disinfection tank, disinfection water for disinfecting sample processing assembly, feed assembly and mixing assembly is arranged in the disinfection tank.

[0008] Preferably, the top of the sample processing box and reagent processing box is slidably connected with sealing cover, driving motor is installed in the inner wall of sealing cover, the output end of driving motor in the sealing cover on sample processing box is fixedly connected with crushing rod through shaft coupling, and the crushing rod is rotatably connected to the bottom of sealing cover.

[0009] The output end of the driving motor in the sealing cover is fixedly connected with an agitating rod through a shaft coupling, and the agitating rod is rotationally connected to the bottom of the sealing cover.

[0010] Preferably, the top of each of the two sealing covers is further provided with a feeding opening, and the feeding opening is in communication with the inside of the sample processing box and the reagent processing box respectively.

[0011] The discharge end of the sample processing box and the reagent processing box is provided with an electromagnetic valve.

[0012] Preferably, the feeding assembly comprises a feeding cylinder, the feeding cylinder is fixedly connected to the inner wall of the pretreatment box, one side of the feeding cylinder is provided with a first servo motor, the output end of the first servo motor is fixedly connected with a feeding auger through a shaft coupling, and the feeding auger is rotationally connected to the feeding cylinder, and a discharging opening is formed in the top of the feeding cylinder close to the side of the first servo motor, and the discharging opening is in communication with the bottom of the sample processing box.

[0013] Preferably, the mixing assembly comprises a mixing box, the mixing box is fixedly connected to the side of the pretreatment box close to the reagent processing box, the mixing box is provided with a feeding opening, the mixing box is in communication with the feeding cylinder through the feeding opening, one side of the mixing box is further provided with a second servo motor, the output end of the second servo motor is fixedly connected with a mixing rod through a shaft coupling, and the top of the mixing box is further provided with a communication pipe, and the two ends of the communication pipe are in communication with the discharge end of the mixing box and the reagent processing box respectively.

[0014] Preferably, the mixing box is further provided with a water pump, the output end of the water pump is communicated with a liquid outlet pipe, and the end of the liquid outlet pipe away from the water pump penetrates the mixing box and the pretreatment box in sequence and extends to the outside of the pretreatment box, and the extending end of the liquid outlet pipe is slidably connected to the side wall of the pretreatment box.

[0015] Compared with the prior art, the utility model has the advantages that:

[0016] 1、The crushing rod and the agitating rod are rotated by the driving motor respectively, the sample is crushed, and the reagent is mixed, the efficient rotation of the crushing rod can rapidly crush the sample, and the subsequent detection and analysis are facilitated, the rotation of the agitating rod ensures the uniform mixing of the detection reagent in the reagent processing box, and the stratification or precipitation of the reagent is avoided, the processing efficiency of the sample and the reagent is improved, and a reliable foundation is provided for the subsequent microbial detection.

[0017] 2. The utility model discloses a disinfection box, when the detection work is completed, can make the disinfectant along disinfectant pipe into sample processing box and reagent processing box and disinfect through opening disinfectant pipe, avoid the cross -contamination of the subsequent detection to other sample, guarantee the accuracy of each detection.

[0018] 3. The utility model discloses a feed auger driven by servo motor, realizes the efficient delivery of sample from sample processing box to mixing box, the rotation of feed auger in feed cylinder can continuously push the sample falling in it forward until entering mixing box, this design not only simplifies the delivery process of sample, but also avoids the loss and pollution of sample in the delivery process.

[0019] 4. The utility model discloses the independent design of sample processing box and reagent processing box, realizes the simultaneous processing of sample and detection reagent, this design not only avoids the cross -contamination of sample and reagent in the adding process, but also improves the accuracy of sample processing and the mixing uniformity of reagent, and the user can conveniently add sample and reagent through respective charging port, and the operation is simple and quick. DRAWINGS

[0020] Fig. 1 It is the first visual angle schematic drawing of the utility model;

[0021] Fig. 2 It is the internal structure schematic drawing of the preprocessing box in the utility model;

[0022] Fig. 3 It is the internal structure schematic drawing of sample processing box in the utility model;

[0023] Fig. 4 It is the internal structure schematic drawing of reagent processing box in the utility model;

[0024] Fig. 5 It is the structure schematic drawing of feed assembly in the utility model;

[0025] Fig. 6 It is the structure schematic drawing of mixing assembly in the utility model.

[0026] In the drawing: 1, preprocessing box;11, first processing cavity;12, second processing cavity;13, cabinet door;2, sample processing assembly;21, sample processing box;22, reagent processing box;23, crushing rod;24, stirring rod;25, sealing cover;26, charging port;27, disinfectant pipe;3, feed assembly;31, feed cylinder;32, first servo motor;33, feed auger;4, mixing assembly;41, mixing box;42, feed inlet;43, second servo motor;44, mixing rod;45, liquid outlet pipe;46, water pump;5, disinfection box. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0028] Embodiment one, please refer to Figs. 1-6 A microbial detection pretreatment device, comprising a pretreatment box 1, the first treatment cavity 11 and the second treatment cavity 12 are formed in the pretreatment box 1, the cabinet door 13 is hinged on the first treatment cavity 11 and the second treatment cavity 12, the sample processing assembly 2 is arranged in the first treatment cavity 11 and the second treatment cavity 12, the sample processing assembly 2 comprises a sample processing box 21 and a reagent processing box 22, the sample processing box 21 and the reagent processing box 22 are arranged in the first treatment cavity 11 and the second treatment cavity 12 respectively, the pretreatment box 1 is also provided with a material conveying assembly 3 and a mixing assembly 4, the feeding end of the material conveying assembly 3 is communicated with the bottom of the sample processing box 21, the feeding end of the mixing assembly 4 is communicated with the reagent processing box 22, and the discharging end of the material conveying assembly 3 is communicated with the mixing assembly 4, and the top of the pretreatment box 1 is also provided with a disinfection box 5, the disinfection box 5 is provided with disinfectant water for disinfecting the sample processing assembly 2, the material conveying assembly 3 and the mixing assembly 4;

[0029] When the collected sample needs to be processed, the sample is first added into the sample processing box 21, the sample is crushed through the sample processing box 21, and the reagent is added in the reagent processing box 22, and various reagents are mixed uniformly through the reagent processing box 22;

[0030] When the sample in the sample processing box 21 is crushed, the material conveying assembly 3 is started, the sample in the sample processing box 21 is conveyed into the mixing assembly 4 through the material conveying assembly 3, the mixed reagent in the reagent processing box 22 is injected into the mixing assembly 4, the mixing assembly 4 is started, the sample and the reagent are mixed uniformly through the mixing assembly 4, and the mixed detection sample is guided out into a culture dish, and the microorganism is cultured and detected.

[0031] Embodiment two, please refer to Figs. 1-4The top of the sample processing box 21 and the reagent processing box 22 is slidably connected with a sealing cover 25, a driving motor is installed in the inner wall of the sealing cover 25, the output end of the driving motor in the sealing cover 25 on the sample processing box 21 is fixedly connected with a crushing rod 23 through a shaft coupling, the crushing rod 23 is rotatably connected to the bottom of the sealing cover 25, the output end of the driving motor in the other sealing cover 25 is fixedly connected with a stirring rod 24 through a shaft coupling, the stirring rod 24 is rotatably connected to the bottom of the sealing cover 25, the top of the two sealing covers 25 is also installed with a feeding port 26, and the two feeding ports 26 are respectively connected with the inside of the sample processing box 21 and the reagent processing box 22, the top of the two sealing covers 25 is also installed with a disinfectant pipe 27, one end of the two disinfectant pipes 27 is respectively connected with the disinfection box 5, the other end penetrates through the adjacent sealing cover 25 and is respectively connected with the inside of the sample processing box 21 and the reagent processing box 22, and the discharge end of the sample processing box 21 and the reagent processing box 22 is provided with an electromagnetic valve;

[0032] When the sample is processed, first, the sample and the detection reagent are respectively added into the sample processing box 21 and the reagent processing box 22 through the feeding ports 26 on the sample processing box 21 and the reagent processing box 22, the driving motors in the sealing covers 25 are started, the two driving motors drive the crushing rod 23 and the stirring rod 24 to rotate respectively, the sample in the sample processing box 21 is crushed through the rotation of the crushing rod 23, and the detection reagent in the reagent processing box 22 is uniformly mixed through the rotation of the stirring rod 24;

[0033] After the sample and the reagent are prepared, the electromagnetic valves at the discharge end of the sample processing box 21 and the reagent processing box 22 are opened, and the feeding assembly 3 and the mixing assembly 4 are started, so that the sample in the sample processing box 21 is input into the mixing assembly 4 through the feeding assembly 3, the reagent in the reagent processing box 22 enters the mixing assembly 4, and the sample is uniformly mixed in the mixing assembly 4;

[0034] When the mixed sample is taken out from the mixing assembly 4, the disinfection box 5 is started, the disinfectant in the disinfection box 5 is continuously injected into the sample processing box 21 and the reagent processing box 22 through the two disinfectant pipes 27, the sample processing box 21 and the reagent processing box 22 are disinfected, and the disinfectant enters the feeding assembly 3 and the mixing assembly 4 through the discharge end of the sample processing box 21 and the reagent processing box 22, so that the feeding assembly 3 and the mixing assembly 4 are disinfected, and the disinfected disinfectant is guided out of the mixing assembly 4.

[0035] Example three, please refer to Figs. 1-6The feeding assembly 3 comprises a feeding cylinder 31 fixedly connected to the inner wall of the pretreatment box 1, one side of the feeding cylinder 31 is provided with a first servo motor 32, the output end of the first servo motor 32 is fixedly connected with a feeding auger 33 through a shaft coupling, and the feeding auger 33 is rotatably connected to the feeding cylinder 31, a feeding opening is formed in the top of the feeding cylinder 31 close to one side of the first servo motor 32, and the feeding opening is in communication with the bottom of the sample treatment box 21, the mixing assembly 4 comprises a mixing box 41 fixedly connected to one side of the pretreatment box 1 close to the reagent treatment box 22, the mixing box 41 is provided with an inlet 42, the mixing box 41 is in communication with the feeding cylinder 31 through the inlet 42, one side of the mixing box 41 is further provided with a second servo motor 43, the output end of the second servo motor 43 is fixedly connected with a mixing rod 44 through a shaft coupling, the top of the mixing box 41 is further provided with a communication pipe, and the two ends of the communication pipe are in communication with the mixing box 41 and the outlet end of the reagent treatment box 22 respectively, the mixing box 41 is further provided with a water pump 46, the output end of the water pump 46 is communicated with a liquid outlet pipe 45, one end of the liquid outlet pipe 45 away from the water pump 46 penetrates the mixing box 41 and the pretreatment box 1 in sequence and extends to the outside of the pretreatment box 1, and the extending end of the liquid outlet pipe 45 is slidably connected to the side wall of the pretreatment box 1;

[0036] When the samples and reagents in the sample treatment box 21 and the reagent treatment box 22 are ready, the electromagnetic valves at the outlet ends of the sample treatment box 21 and the reagent treatment box 22 are opened, and the first servo motor 32 and the second servo motor 43 are started, the samples in the sample treatment box 21 enter the feeding cylinder 31 through the outlet end, the feeding auger 33 in the feeding cylinder 31 rotates in the feeding cylinder 31 under the drive of the first servo motor 32, and the samples falling in the feeding cylinder 31 are continuously conveyed forward to the mixing box 41 through the rotation of the feeding auger 33, so that the samples enter the mixing box 41 through the inlet 42;

[0037] At the same time, the communication pipe on the mixing box 41 inputs the detection reagent in the reagent treatment box 22 into the mixing box 41, when the second servo motor 43 rotates, the mixing rod 44 in the mixing box 41 rotates under the action of the second servo motor 43, so that the samples and the detection reagent falling in the mixing box 41 are uniformly mixed;

[0038] After mixing, the water pump 46 is started, the samples and reagents in the mixing box 41 are pumped out through the water pump 46, and are discharged outward through the liquid outlet pipe 45.

[0039] Working principle: when the sample is processed, firstly, the sample and the detection reagent are respectively added into the sample processing box 21 and the reagent processing box 22 through the feeding opening 26 on the sample processing box 21 and the reagent processing box 22, the driving motor in the sealing cover 25 is started, the two driving motors drive the crushing rod 23 and the stirring rod 24 to rotate respectively, the sample in the sample processing box 21 is crushed through the rotation of the crushing rod 23, and the detection reagent in the reagent processing box 22 is uniformly mixed through the rotation of the stirring rod 24;

[0040] After the sample and the reagent are prepared, the electromagnetic valve at the discharge end of the sample processing box 21 and the reagent processing box 22 is opened, and the first servo motor 32 and the second servo motor 43 are started, the sample in the sample processing box 21 enters the feeding cylinder 31 through the discharge end, the feeding auger 33 in the feeding cylinder 31 rotates in the feeding cylinder 31 under the action of the first servo motor 32, and the sample falling in the feeding cylinder 31 is continuously conveyed to the mixing box 41 through the rotation of the feeding auger 33, so that the sample enters the mixing box 41 through the feeding opening 42;

[0041] Meanwhile, the communication pipe on the mixing box 41 inputs the detection reagent in the reagent processing box 22 into the mixing box 41, when the second servo motor 43 rotates, the mixing rod 44 in the mixing box 41 rotates under the action of the second servo motor 43, so that the sample and the detection reagent falling in the mixing box 41 are uniformly mixed;

[0042] After mixing, the water pump 46 is started, the sample reagent in the mixing box 41 is pumped out through the water pump 46, and is guided outwards through the liquid outlet pipe 45.

[0043] It should be noted that the devices in the present application are common market devices, which can be selected according to the needs during use, and the circuit connection relationship of each device is a simple series and parallel connection circuit, and there is no innovation point in the circuit connection part, which can be easily realized by the person skilled in the art, and belongs to the prior art, which will not be described here.

[0044] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and modifications can be made to these embodiments without departing from the principles and spirits of the present application, the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A microbial detection pre-treatment device comprising a pre-treatment tank (1), characterized in that: The first processing cavity (11) and the second processing cavity (12) are hingedly connected with cabinet doors (13), and a sample processing assembly (2) is arranged in the first processing cavity (11) and the second processing cavity (12). The top of the pre-treatment box (1) is also provided with a disinfection box (5), and the disinfection box (5) is provided with disinfectant water for disinfecting the sample processing assembly (2), the material conveying assembly (3) and the mixing assembly (4).

2. The pre-treatment device for microorganism detection according to claim 1, characterized in that: The top of the sample processing box (21) and the reagent processing box (22) is slidably connected with a sealing cover (25), and a drive motor is arranged in the inner wall of the sealing cover (25). The output end of the drive motor in the other sealing cover (25) is fixedly connected with an agitating rod (24) through a shaft coupling, and the agitating rod (24) is rotatably connected to the bottom of the sealing cover (25).

3. The pre-treatment device for microorganism detection according to claim 2, wherein: The top of the two sealing covers (25) is also provided with a feeding port (26), and the two feeding ports (26) are respectively connected with the interiors of the sample processing box (21) and the reagent processing box (22). The discharge end of the sample processing box (21) and the reagent processing box (22) is provided with an electromagnetic valve.

4. The pre-treatment device for microorganism detection according to claim 1, wherein: The material conveying assembly (3) comprises a material conveying cylinder (31) fixedly connected in the inner wall of the pre-treatment box (1), a first servo motor (32) mounted on one side of the material conveying cylinder (31), a material conveying auger (33) fixedly connected to the output end of the first servo motor (32) through a shaft coupling and rotatably connected in the material conveying cylinder (31), and a material falling port formed in the top of the material conveying cylinder (31) near the side of the first servo motor (32), and the material falling port is connected with the bottom of the sample processing box (21).

5. The pre-treatment device for microorganism detection according to claim 1, wherein: The mixing assembly (4) comprises a mixing box (41) fixedly connected to one side of the pretreatment box (1) close to the reagent treatment box (22), a feeding port (42) is formed in the mixing box (41), the mixing box (41) is communicated with the feeding cylinder (31) through the feeding port (42), a second servo motor (43) is further installed on one side of the mixing box (41), the output end of the second servo motor (43) is fixedly connected with a mixing rod (44) through a shaft coupling, and a communication pipe is further arranged on the top of the mixing box (41) and communicated with the mixing box (41) and the discharge end of the reagent treatment box (22).

6. The pre-treatment device for microorganism detection according to claim 5, wherein: A water pump (46) is further installed in the mixing box (41), the output end of the water pump (46) is communicated with a liquid outlet pipe (45), one end of the liquid outlet pipe (45) away from the water pump (46) penetrates the mixing box (41) and the pretreatment box (1) in sequence and extends to the outside of the pretreatment box (1), and the extending end of the liquid outlet pipe (45) is slidably clamped on the side wall of the pretreatment box (1).

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