Biological cell settling tank

Abstract

The utility model relates to a biological cell settling tank which comprises a collecting tank, a connecting seat at the bottom of the collecting tank is inserted into an output shaft, an alignment groove at the bottom of the connecting seat is fixedly clamped with an alignment block at the top of the output shaft, and suspension liquid is poured into the collecting tank. When the liquid flows into the four storage pipes, the motor is started to drive the output shaft to rotate, so that the collecting tank is driven to rotate, cells in the suspension are settled and fall into the bottoms of the four storage pipes, and waste liquid is positioned above the storage pipes; the flow pipe and the storage pipes are in threaded installation, the rotary storage pipes are detached from the flow pipe, and the sealing cover and the tops of the storage pipes are in threaded installation; the top of the storage tube is sealed, then the sealing cap is rotated, the storage tube is similar to an injector, the piston block is driven to move by pressing the pushing rod, the surface of the piston block is tightly attached to the inner wall of the tube body, and therefore cells at the bottom of the storage tube are pushed out and collected, and the remaining part is waste liquid.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell sedimentation equipment technical field, concretely is a kind of biological cell sedimentation tank. BACKGROUND

[0002] The working principle of biological cell sedimentation tank is based on gravity sedimentation principle. Under the action of gravity, due to the density difference between solid (cells) and liquid (culture medium), solid is deposited at the bottom, and clear liquid is overflowed from the upper part of the tank along the periphery and discharged. In the prior art, the static suspension liquid can cause uneven distribution of cells in the storage tank, with dense cells in some areas and sparse cells in other areas. When the cell sedimentation mainly relies on gravity, the sedimentation process is slow.

[0003] For example, the authorized patent document with application number CN202220399977.5 discloses a centrifugal sedimentation tube for extracting cells, which includes a sedimentation tube body and a support mechanism. The support mechanism includes an operating table, a support box, a support groove, a first damping hole, a first clamping arc plate, and a second clamping arc plate. The first clamping arc plate is provided with a first connecting L plate, and the second clamping arc plate is provided with a second connecting L plate. The top end of the first connecting L plate and the bottom end of the second connecting L plate are both provided with a first tooth plate. The first damping hole is provided with a first damping shaft, and the first damping shaft is provided with a first gear. The operating table is provided with a lifting mechanism.

[0004] The above-mentioned patent has the problem that the static suspension liquid can cause uneven distribution of cells in the storage tank, with dense cells in some areas and sparse cells in other areas. Therefore, we need to provide a biological cell sedimentation tank. INVENTION CONTENTS

[0005] The utility model aims at providing a biological cell sedimentation tank, which is installed with a rotating sedimentation mechanism at the bottom of the collection tank to rotate the suspension liquid in the collection tank, helping the cells to be more uniformly distributed in the liquid. Through rotation, the cells in the suspension liquid will be subjected to a more uniform force field, which helps to accelerate the sedimentation process of the cells, thereby improving the sedimentation effect and cell recovery rate, and solving the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a biological cell sedimentation tank, comprising:

[0007] a collection tank;

[0008] The bottom of the collection tank is provided with a rotating sedimentation mechanism for rotating the collection tank to rotate the suspension liquid in the collection tank and accelerate the cell sedimentation.

[0009] The four corners of the bottom of the collection tank are each installed with a cell separation mechanism for separating the cells in the suspension liquid from the waste liquid.

[0010] Preferably, the bottom of the collecting tank is provided with a control console, the bottom of the collecting tank is fixedly installed with a connecting seat, the rotating sedimentation mechanism is installed in the control console, and the rotating sedimentation mechanism is used in cooperation with the connecting seat.

[0011] Preferably, the bottom of the collecting tank is communicated with four flow pipes, the bottom of each of the four flow pipes is communicated with a cell separation mechanism, and the surface of each of the four flow pipes is fixedly installed with a sealing ring.

[0012] Preferably, the cell separation mechanism comprises a storage pipe which is screw-installed on the surface of the flow pipe, the bottom of the storage pipe is communicated with a discharge pipe, the bottom of the discharge pipe is screw-installed with a sealing cap, one side of the storage pipe is communicated with a pushing piece, and the pushing piece is used for discharging cells in the storage pipe.

[0013] Preferably, the top of the control console is provided with a placing groove, a plurality of sealing covers are arranged in the placing groove, the sealing covers are used for sealing the top of the storage pipe, the sealing covers are screw-installed on the top of the storage pipe, and the sealing covers are used in cooperation with the pushing piece.

[0014] Preferably, the pushing piece comprises a pipe body which is communicated with one side of the storage pipe, a piston block is installed in the pipe body, the surface of the piston block is tightly combined with the inner wall of the pipe body, a pushing rod is fixedly installed on one side of the piston block, and one end of the pushing rod penetrates through one side of the pipe body and extends.

[0015] Preferably, the rotating sedimentation mechanism comprises a motor which is installed in the control console, an output shaft is installed at the output end of the motor, a positioning block is fixedly installed on the top of the output shaft, and the bottom of the connecting seat is provided with a positioning groove for inserting the positioning block.

[0016] Preferably, the bottom of the inner wall of the collecting tank is fixedly installed with a conical flow divider.

[0017] Compared with the prior art, the beneficial effects of the utility model are:

[0018] The utility model discloses a rotating sedimentation mechanism is installed at the bottom of the collecting tank, and the suspension in the collecting tank is rotated, which helps cells to be more uniformly distributed in the liquid. Through rotation, cells in the suspension are subjected to a more uniform force field, which helps to accelerate the sedimentation process of the cells, thereby improving the sedimentation effect and the cell recovery rate. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is the structure of the utility model three -dimensional schematic diagram;

[0020] Figure 2 It is the rotating sedimentation mechanism three -dimensional drawing of the utility model;

[0021] Figure 3 It is a perspective view of the conical flow divider of the utility model;

[0022] Figure 4 It is a perspective view of the local structure of the utility model;

[0023] Figure 5 It is a perspective view of the push pressure piece of the utility model.

[0024] In the drawing: 1, collecting tank; 2, rotating sedimentation mechanism; 21, motor; 22, output shaft; 23, alignment block; 24, alignment groove; 3, cell separation mechanism; 31, storage tube; 32, discharge pipe; 33, sealing cap; 30, push pressure piece; 301, tube body; 302, piston block; 303, push rod; 4, control console; 5, connecting seat; 6, flow pipe; 7, sealing ring; 8, placing groove; 9, sealing cover; 10, conical flow divider. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0026] Please refer to Figures 1-5 The utility model provides a technical scheme: a biological cell sedimentation tank, comprising:

[0027] Collecting tank 1;

[0028] The bottom of the collecting tank 1 is provided with a rotating sedimentation mechanism 2 for rotating the collecting tank 1, realizing rotation of the suspension liquid in the collecting tank 1 and accelerating cell sedimentation.

[0029] The four corners of the bottom of the collecting tank 1 are each provided with a cell separation mechanism 3 for separating cells from waste liquid in the suspension liquid.

[0030] Specifically, the rotating sedimentation mechanism 2 is installed at the bottom of the collecting tank 1 to rotate the suspension liquid in the collecting tank 1, which helps the cells to be more uniformly distributed in the liquid. Through rotation, the cells in the suspension liquid are subjected to a more uniform force field, which helps to accelerate the cell sedimentation process, thereby improving the sedimentation effect and cell recovery rate.

[0031] The bottom of the collecting tank 1 is provided with a control console 4, and the bottom of the collecting tank 1 is fixedly provided with a connecting seat 5. The rotating sedimentation mechanism 2 is installed in the control console 4 and is used in cooperation with the connecting seat 5.

[0032] In the embodiment, the connecting seat 5 at the bottom of the collecting tank 1 is inserted on the output shaft 22, the bottom of the connecting seat 5 is clamped and fixed with the positioning block 23 at the top of the output shaft 22, the suspension is poured into the collecting tank 1, and the suspension is dispersed at the edge of the conical flow divider 10 due to the conical flow divider 10 arranged in the collecting tank 1. The liquid flows into the four storage tubes 31, the motor 21 is started to drive the output shaft 22 to rotate, thereby driving the collecting tank 1 to rotate, so that the cells in the suspension are settled and fall into the bottom of the four storage tubes 31, and the waste liquid is located above the storage tubes 31. Since the flow pipe 6 is screw-mounted with the storage tube 31, the storage tube 31 is detached from the flow pipe 6 by rotating the storage tube 31, the sealing cover 9 is screw-mounted with the top of the storage tube 31, the top of the storage tube 31 is sealed, and then the sealing cap 33 is rotated. At this time, the storage tube 31 is similar to a syringe, the piston block 302 is moved by pressing the push rod 303, and the surface of the piston block 302 is tightly fitted with the inner wall of the tube body 301, so that the cells at the bottom of the storage tube 31 are pushed out first, and the remaining part is waste liquid.

[0033] The bottom of the collecting tank 1 is communicated with four flow pipes 6, the bottoms of the four flow pipes 6 are communicated with the cell separation mechanism 3, and the surfaces of the four flow pipes 6 are fixedly installed with sealing rings 7.

[0034] Specifically, the sealing rings 7 are fixedly installed on the surfaces of the flow pipes 6, when the flow pipe 6 is connected with the storage tube 31, the top of the storage tube 31 is fitted with the sealing ring 7, thereby enhancing the sealing effect.

[0035] The cell separation mechanism 3 comprises the storage tube 31 screw-mounted on the surface of the flow pipe 6, the bottom of the storage tube 31 is communicated with the discharge pipe 32, the bottom of the discharge pipe 32 is screw-mounted with the sealing cap 33, one side of the storage tube 31 is communicated with the pusher 30, and the pusher 30 is used for discharging the cells in the storage tube 31.

[0036] Further, the sealing cover 9 is screw-mounted with the top of the storage tube 31, the top of the storage tube 31 is sealed, and then the sealing cap 33 is rotated. At this time, the storage tube 31 is similar to a syringe, the piston block 302 is moved by pressing the push rod 303, and the surface of the piston block 302 is tightly fitted with the inner wall of the tube body 301, so that the cells at the bottom of the storage tube 31 are pushed out first.

[0037] The top of the console 4 is provided with a placing groove 8, a plurality of sealing covers 9 are arranged in the placing groove 8, the sealing covers 9 are used for sealing the top of the storage tube 31, the sealing covers 9 are screw-mounted with the top of the storage tube 31, and the sealing covers 9 are used in cooperation with the pusher 30.

[0038] The placing groove 8 is arranged for collecting a plurality of sealing covers 9, the sealing covers 9 are screw-mounted with the top of the storage tube 31, and the sealing cap 33 is removed. At this time, the storage tube 31 is similar to a syringe.

[0039] The pushing piece 30 comprises a pipe body 301 communicated at one side of the storage pipe 31, the inside of the pipe body 301 is provided with a piston block 302, the surface of the piston block 302 is tightly combined with the inner wall of the pipe body 301, one side of the piston block 302 is fixedly provided with a pushing rod 303, one end of the pushing rod 303 penetrates through one side of the pipe body 301 and extends;

[0040] It should be noted that the piston block 302 is moved by pressing the pushing rod 303, and the surface of the piston block 302 is tightly combined with the inner wall of the pipe body 301, so that the cells at the bottom of the storage pipe 31 are pushed out first.

[0041] The rotating settling mechanism 2 comprises a motor 21 installed in the console 4, the output end of the motor 21 is provided with an output shaft 22, the top of the output shaft 22 is fixedly provided with a positioning block 23, and the bottom of the connecting seat 5 is provided with a positioning groove 24 for inserting the positioning block 23;

[0042] It should be noted that the output shaft 22 is driven to rotate by starting the motor 21, so that the collection tank 1 is driven to rotate, so that the cells in the suspension liquid fall into the bottoms of the four storage pipes 31, and the waste liquid is located above the storage pipes 31.

[0043] The bottom of the inner wall of the collection tank 1 is fixedly provided with a conical flow divider 10.

[0044] In order to avoid the accumulation of the suspension liquid in the collection tank 1, the conical flow divider 10 is arranged to divide the flow.

[0045] The connecting seat 5 at the bottom of the collection tank 1 is inserted on the output shaft 22, the bottom positioning groove 24 of the connecting seat 5 is clamped and fixed with the top positioning block 23 of the output shaft 22, the suspension liquid is poured into the collection tank 1, and since the conical flow divider 10 is arranged in the collection tank 1, the suspension liquid is dispersed at the edge of the conical flow divider 10, the liquid flows into the four storage pipes 31, the output shaft 22 is driven to rotate by starting the motor 21, so that the collection tank 1 is driven to rotate, so that the cells in the suspension liquid fall into the bottoms of the four storage pipes 31, and the waste liquid is located above the storage pipes 31, since the flow pipe 6 is screw-mounted with the storage pipe 31, the storage pipe 31 is detached from the flow pipe 6 by rotating the storage pipe 31, the sealing cover 9 is screw-mounted with the top of the storage pipe 31, the top of the storage pipe 31 is sealed, then the sealing cap 33 is rotated, at this time, the storage pipe 31 is similar to a syringe, the piston block 302 is moved by pressing the pushing rod 303, and the surface of the piston block 302 is tightly combined with the inner wall of the pipe body 301, so that the cells at the bottom of the storage pipe 31 are pushed out first, and the remaining part is waste liquid.

[0046] Although the embodiments of the present application have been shown and described, it should be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A biological cell settling tank, characterized by, The application relates to a cell collection device. The collection tank (1) is provided with a rotating sedimentation mechanism (2) at the bottom for rotating the collection tank (1) and rotating the suspension in the collection tank (1) to accelerate cell sedimentation. The bottom of the collection tank (1) is provided with a cell separation mechanism (3) at four corners for separating cells from waste liquid in the suspension. The bottom of the collection tank (1) is provided with a control console (4), and the bottom of the collection tank (1) is fixedly provided with a connecting seat (5); the rotating sedimentation mechanism (2) is arranged in the control console (4), and the rotating sedimentation mechanism (2) is used in cooperation with the connecting seat (5).

2. The biological cell settling tank according to claim 1, characterized in that: The bottom of the collection tank (1) is communicated with four flow pipes (6), the bottom of each of the four flow pipes (6) is communicated with the cell separation mechanism (3), and the surface of each of the four flow pipes (6) is fixedly provided with a sealing ring (7).

3. A biological cell settling tank according to claim 2, wherein: The cell separation mechanism (3) comprises a storage pipe (31) which is screw-connected to the surface of the flow pipe (6), the bottom of the storage pipe (31) is communicated with a discharge pipe (32), the bottom of the discharge pipe (32) is screw-connected with a sealing cap (33), one side of the storage pipe (31) is communicated with a pushing piece (30), and the pushing piece (30) is used for discharging cells in the storage pipe (31).

4. A biological cell settling tank according to claim 3, wherein: The top of the control console (4) is provided with a placing groove (8), the inside of the placing groove (8) is provided with a plurality of sealing covers (9), the sealing covers (9) are used for sealing the top of the storage pipe (31), the sealing covers (9) are screw-connected with the top of the storage pipe (31), and the sealing covers (9) are used in cooperation with the pushing piece (30).

5. A biological cell settling tank according to claim 4, wherein: The pushing piece (30) comprises a pipe body (301) which is communicated with one side of the storage pipe (31), the inside of the pipe body (301) is provided with a piston block (302), the surface of the piston block (302) is tightly combined with the inner wall of the pipe body (301), one side of the piston block (302) is fixedly provided with a pushing rod (303), and one end of the pushing rod (303) penetrates through one side of the pipe body (301) and extends.

6. A biological cell settling tank according to claim 5, wherein: The rotating sedimentation mechanism (2) comprises a motor (21) which is arranged in the control console (4), the output end of the motor (21) is provided with an output shaft (22), the top of the output shaft (22) is fixedly provided with a positioning block (23), and the bottom of the connecting seat (5) is provided with a positioning groove (24) for inserting the positioning block (23).

7. The biological cell settling tank of claim 2, wherein: The bottom of the inner wall of the collection tank (1) is fixedly provided with a conical flow divider (10).

8. The biological cell settling tank of claim 1, wherein: ​

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