Multi-channel full-automatic microorganism enrichment instrument

Abstract

The utility model discloses a multi-channel full-automatic microorganism enrichment instrument which comprises a base, a mounting frame, a shell, a sample water bottle, a sample water bottle support, an enrichment pipe, an enrichment pipe support and an eluent storage tank, the mounting frame is arranged on the base and is of a frame structure, the sample water bottle support and the enrichment pipe support are connected to the mounting frame, the sample water bottle is arranged on the sample water bottle support, and the eluent storage tank is arranged on the shell. The enrichment pipe is arranged on the enrichment pipe bracket, the sample water bottle is communicated with the enrichment pipe, the eluent storage tank is communicated with the enrichment pipe, and the shell covers the mounting frame; a control panel is further arranged on the mounting frame; a plurality of sample water bottles and enrichment tubes are arranged, and each sample water bottle corresponds to one enrichment tube; the time, energy and cost of experimenters can be saved.

Description

Technical Field

[0001] This utility model belongs to the field of enrichment equipment technology, and in particular relates to a multi-channel fully automatic microbial enrichment instrument. Background Technology

[0002] When detecting microorganisms, biomolecules, cells, and other substances in liquid samples, the concentration of the target substance determines whether it can be detected. When the concentration of the target substance in the liquid sample is too low, it usually results in the target substance not being detected.

[0003] Bioaccumulation technology utilizes various methods to enrich target substances, removing some of the solvent from liquid samples or transferring the target substance to a new solvent, thereby increasing the overall concentration of the target substance and reducing the difficulty of detection and equipment requirements. Commonly used enrichment methods include magnetic bead methods, chromatography, and filtration. Among them, filtration uses a filter membrane with micro-nano sizes to intercept the target substance, and with the help of external forces such as gravity, air pressure, and mechanical force, the solvent passes through the filter membrane, while the target substance is retained on one side of the filter membrane. Finally, a small amount of eluent is used to elute the target substance, achieving the purpose of concentration and enrichment.

[0004] Existing enrichment processes are complex and time-consuming, requiring researchers to invest significant time and effort in the enrichment process, which delays the acquisition of detection data and impacts the overall project progress. Utility Model Content

[0005] The summary section of this application is intended to provide a brief overview of the concepts, which will be described in detail in the detailed description section below. This summary section is not intended to identify key or essential features of the claimed technical solutions, nor is it intended to limit the scope of the claimed technical solutions.

[0006] In order to overcome the shortcomings of the prior art, this utility model provides a multi-channel fully automatic microbial enrichment instrument.

[0007] To achieve the above objectives, this utility model adopts the following technical solution: a multi-channel fully automatic microbial enrichment instrument, comprising a base, a mounting frame, a shell, sample bottles, sample bottle supports, enrichment tubes, enrichment tube supports, and an eluent storage tank. The mounting frame is mounted on the base and has a frame structure. The sample bottle supports and enrichment tube supports are connected to the mounting frame. The sample bottles are placed on the sample bottle supports, and the enrichment tubes are placed on the enrichment tube supports. The sample bottles and enrichment tubes are connected. The eluent storage tank is connected to the enrichment tubes. The shell covers the mounting frame. The mounting frame also has a control panel. Multiple sample bottles and enrichment tubes are configured, with each sample bottle corresponding to one enrichment tube.

[0008] Furthermore, the enrichment tube has a first convex tube section and a second convex tube section at both ends, the first convex tube section has a sample port, the second convex tube section has a water inlet, and the bottom of the enrichment tube has a water outlet.

[0009] Furthermore, the enrichment tube is equipped with multiple guide wires, which are hollow in structure, with one end closed and the other end connected to the water outlet; the guide wires are made of ultrafiltration material.

[0010] Furthermore, the enrichment tube support is provided with an installation groove, and a clamping clip is provided in the installation groove. The clamping clip is made of elastic material, and the enrichment tube is placed in the clamping clip.

[0011] Furthermore, the bottom of the mounting groove is provided with two vibrating plates, with the first convex tube and the second convex tube respectively placed on the vibrating plates; the enrichment tube support is provided with a motor for driving the vibrating plates to vibrate.

[0012] Furthermore, the enrichment tube support has a magnetic connector at one end and a magnet at the other end, and an enrichment tube mounting plate on the mounting bracket, which is made of ferrous metal.

[0013] Furthermore, the eluent storage tank is connected to a multi-way valve, and a water pump is installed inside the eluent storage tank.

[0014] Furthermore, the mounting frame is also equipped with a pressure regulator, and the side wall of the mounting frame is equipped with a sample tube connector that is connected to the sample water bottle. The sample tube connector is connected to the pressure regulator, which is equipped with an air inlet and a liquid outlet. The enrichment tube is connected to the liquid outlet.

[0015] Furthermore, the mounting bracket is equipped with a PCB board, which has signal wiring terminals. The mounting bracket is also equipped with a connection board that connects to the PCB board, which has a USB interface, a rich socket, and a power socket.

[0016] Furthermore, the mounting rack is also equipped with ultraviolet disinfection lamps.

[0017] The advantage of this invention is that it provides a multi-channel fully automated microbial enrichment instrument that saves laboratory personnel time, effort, and costs. Attached Figure Description

[0018] The accompanying drawings, which form part of this application, are used to provide a further understanding of the application and to make other features, objects, and advantages of the application more apparent. The illustrative embodiments and descriptions of this application are used to explain the application and do not constitute an undue limitation of the application.

[0019] Furthermore, throughout the accompanying drawings, the same or similar reference numerals denote the same or similar elements. It should be understood that the drawings are schematic, and the elements are not necessarily drawn to scale.

[0020] In the attached diagram:

[0021] Figure 1 This is a schematic diagram of the structure of a multi-channel fully automated microbial enrichment instrument in one embodiment of the present invention.

[0022] Figure 2 for Figure 1 A schematic diagram of the multi-channel fully automated microbial enrichment instrument without its outer shell shown in the embodiment.

[0023] Figure 3 for Figure 1 A schematic diagram of the rear left side of the multi-channel fully automated microbial enrichment instrument in the illustrated embodiment.

[0024] Figure 4 for Figure 1 A schematic diagram of the enrichment support and enrichment tube of the multi-channel fully automated microbial enrichment instrument shown in the embodiment.

[0025] Figure 5 for Figure 1 A schematic diagram of the rear right side of the multi-channel fully automated microbial enrichment instrument in the illustrated embodiment.

[0026] The meanings of the reference numerals in the figure are as follows:

[0027] 1. Base; 2. Housing; 3. Sample bottle; 4. Control panel; 5. Enrichment tube support; 6. Mounting bracket; 7. Sample bottle support; 8. Elution buffer storage tank; 9. Enrichment tube mounting plate; 10. Voltage regulator; 11. Air inlet; 12. Drain outlet; 13. Multi-way valve; 14. Drain valve; 15. Sample tube connector; 16. Ultraviolet disinfection lamp; 17. Enrichment tube; 171. Sample port; 172. Water inlet; 173. Water outlet; 18. Vibrating plate; 19. Magnetic connector; 20. Magnet; 21. PCB board; 22. Signal terminal block; 23. USB interface; 24. Enrichment socket; 25. Power socket. Detailed Implementation

[0028] Embodiments of this disclosure will now be described in more detail with reference to the accompanying drawings. While some embodiments of this disclosure are shown in the drawings, it should be understood that this disclosure can be implemented in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided to provide a more thorough and complete understanding of this disclosure. It should be understood that the accompanying drawings and embodiments of this disclosure are for illustrative purposes only and are not intended to limit the scope of protection of this disclosure.

[0029] It should also be noted that, for ease of description, only the parts relevant to the utility model are shown in the accompanying drawings. Unless otherwise specified, the embodiments and features described in this disclosure can be combined with each other.

[0030] It should be noted that the concepts of "first" and "second" mentioned in this disclosure are used only to distinguish different devices, modules or units, and are not used to limit the order of functions performed by these devices, modules or units or their interdependencies.

[0031] It should be noted that the terms "a" and "a plurality of" used in this disclosure are illustrative rather than restrictive, and those skilled in the art should understand that, unless otherwise expressly indicated in the context, they should be understood as "one or more".

[0032] The names of messages or information exchanged between multiple devices in the embodiments of this disclosure are for illustrative purposes only and are not intended to limit the scope of such messages or information.

[0033] This disclosure will now be described in detail with reference to the accompanying drawings and embodiments.

[0034] like Figure 1-5 As shown, a multi-channel fully automated microbial enrichment instrument includes a base 1, a mounting frame 6, a housing 2, sample bottles 3, sample bottle supports 7, enrichment tubes 17, enrichment tube supports 5, and an eluent storage tank 8. The mounting frame 6 is mounted on the base 1 and has a frame structure. The sample bottle supports 7 and enrichment tube supports 5 are connected to the mounting frame 6. The sample bottles 3 are placed on the sample bottle supports 7, and the enrichment tubes 17 are placed on the enrichment tube supports 5. The sample bottles 3 and enrichment tubes 17 are connected. The eluent storage tank 8 is connected to the enrichment tubes 17. The housing 2 covers the mounting frame 6. The mounting frame 6 is divided into left and right parts: the left side is the process handling section, and the right side is the control section. The right side of the frame 6 is equipped with a control panel 4; multiple sample bottles 3 and enrichment tubes 17 are configured, with each sample bottle 3 corresponding to one enrichment tube 17; each sample bottle 3 and enrichment tube 17 is set independently and does not interfere with each other, so that each set of sample bottles 3 and enrichment tubes 17 can be used independently, and multiple sets of water samples can be enriched at the same time, further reducing the time cost required for enrichment; the control panel 4 can be used to control the use of the entire enrichment instrument. After the water sample is injected into the sample bottle 3, simply place the corresponding enrichment tube 17 and set the program to automatically complete the enrichment operation of the water sample, making the enrichment operation automated, freeing up manpower, and convenient to use.

[0035] The enrichment tube 17 has a first convex tube section and a second convex tube section at both ends. The first convex tube section has a sample port 171, and the second convex tube section has a water inlet 172. The bottom of the enrichment tube 17 has a water outlet 173. The enrichment tube 17 contains multiple guide wires. The guide wires are hollow, with one end closed and the other end connected to the water outlet 173. The guide wires are made of ultrafiltration material. This product is designed to process large-volume, low-concentration water samples. The water sample enters the enrichment tube 17 through the sample port 171. The water flows through the side wall of the guide wire and into the guide wire. The water flows out from the water outlet 173. Microorganisms are filtered out outside the guide wire and remain in the enrichment tube 17. The eluent enters the enrichment tube 17 multiple times through the water inlet 172 to elute the microorganisms in the enrichment tube 17, thus completing the enrichment treatment of the water sample.

[0036] The enrichment tube support 5 is provided with an installation groove, and a clamping clip is provided in the installation groove. The clamping clip is made of plastic. The enrichment tube 17 is placed in the clamping clip and the clamping clip is used to fix the enrichment tube 17 in the installation groove. At the same time, the enrichment tube 17 can be directly peeled off from the clamping clip, and the enrichment tube 17 can be quickly replaced, reducing the difficulty of replacing the enrichment tube 17.

[0037] Furthermore, two vibrating plates 18 are provided at the bottom of the mounting groove, with the first and second convex tube portions respectively placed on the vibrating plates 18; the enrichment tube support 5 is equipped with a motor for driving the vibrating plates 18 to vibrate; one end of the enrichment tube support 5 is equipped with a magnetic connector 19, and the other end is equipped with a magnet 20; the mounting bracket 6 is equipped with an enrichment tube mounting plate 9, which is made of ferrous metal; the enrichment tube 17 is attracted to the enrichment tube mounting plate 9 by the magnet 20 and the magnetic connector 19. The installation of the enrichment tube support 5 can be quickly completed by simply bringing the enrichment tube support 5 close to the enrichment tube mounting plate 9, and the enrichment tube support 5 can also be directly removed from the enrichment tube mounting plate 9 for quick replacement of the enrichment tube 17. The operation further reduces the difficulty of replacing the enrichment tube 17; a cam is connected to the output shaft of the motor, and the cam intermittently impacts the vibrating plate 18 during rotation, causing the vibrating plate 18 to vibrate. Since the first and second convex tubes are in contact with the vibrating plate 18, the vibration of the vibrating plate 18 is transmitted to the enrichment tube 17, causing the enrichment tube 17 to vibrate, thereby improving the elution effect; the two vibrating plates 18 have different vibration frequencies, causing the enrichment tube 17 to intermittently generate two different vibrations, further improving the elution effect of the enrichment tube 17; the magnetic connector 19 ensures that the motor is always powered on, guaranteeing stable operation of the motor.

[0038] The eluent storage tank 8 is connected to a multi-way valve 13. The eluent storage tank 8 is equipped with a water pump. The multi-way valve 13 is used to control the flow direction of the eluent so as to send the eluent into different enrichment tubes 17. The water pump provides power for the flow of the eluent to complete the elution operation.

[0039] The mounting frame 6 is also equipped with a pressure regulator 10. The side wall of the mounting frame 6 is equipped with a sample tube connector 15 that is connected to the sample water bottle 3. The sample tube connector 15 is connected to the pressure regulator 10. The pressure regulator 10 is equipped with an air inlet 11 and a drain outlet 12. The sample port 171 on the enrichment tube 17 is connected to the drain outlet 12. The base 1 is equipped with a drain valve 14, which is connected to the water outlet 173 of the enrichment tube 17 to control the opening and closing of the enrichment tube 17.

[0040] The mounting bracket 6 has a PCB board 21, which has signal wiring terminals 22. The control panel 4 is connected to the PCB board 21. The mounting bracket 6 also has a connection plate connected to the PCB board 21, which has a USB interface 23, a rich socket 24, and a power socket 25.

[0041] The mounting bracket 6 is also equipped with an ultraviolet disinfection lamp 16, which is used to disinfect the inside of the enrichment instrument.

[0042] The above description is merely a selection of preferred embodiments of this disclosure and an explanation of the technical principles employed. Those skilled in the art should understand that the scope of the utility model involved in the embodiments of this disclosure is not limited to the technical solutions formed by specific combinations of the above-described technical features, but should also cover other technical solutions formed by arbitrary combinations of the above-described technical features or their equivalents without departing from the above-described inventive concept. For example, technical solutions formed by substituting the above features with (but not limited to) technical features with similar functions disclosed in the embodiments of this disclosure.

Claims

1. A multi-channel fully automated microbial enrichment instrument, characterized in that: The device includes a base, a mounting frame, a housing, sample bottles, sample bottle supports, enrichment tubes, enrichment tube supports, and an eluent storage tank. The mounting frame is mounted on the base and has a frame structure. The sample bottle supports and enrichment tube supports are connected to the mounting frame. The sample bottles are placed on the sample bottle supports, and the enrichment tubes are placed on the enrichment tube supports. The sample bottles and enrichment tubes are connected in communication. The eluent storage tank is connected in communication with the enrichment tubes. The housing covers the mounting frame. The mounting frame also has a control panel. Multiple sample bottles and enrichment tubes are provided, with each sample bottle corresponding to one enrichment tube.

2. The multi-channel fully automated microbial enrichment instrument according to claim 1, characterized in that: The enrichment tube has a first convex tube section and a second convex tube section at both ends, the first convex tube section has a sample port, the second convex tube section has a water inlet, and the bottom of the enrichment tube has a water outlet.

3. The multi-channel fully automated microbial enrichment instrument according to claim 2, characterized in that: The enrichment tube is equipped with multiple guide wires, which are hollow in structure. One end of the guide wire is closed and the other end is connected to the water outlet. The guide wires are made of ultrafiltration material.

4. The multi-channel fully automated microbial enrichment instrument according to claim 2, characterized in that: The enrichment tube support is provided with a mounting groove, and a clamping clip is provided in the mounting groove. The clamping clip is made of elastic material, and the enrichment tube is placed in the clamping clip.

5. The multi-channel fully automated microbial enrichment instrument according to claim 4, characterized in that: The bottom of the mounting groove is provided with two vibrating plates, and the first convex tube and the second convex tube are respectively placed on the vibrating plates; the enrichment tube support is provided with a motor for driving the vibrating plates to vibrate.

6. The multi-channel fully automated microbial enrichment instrument according to claim 5, characterized in that: The enrichment tube support is provided with a magnetic connector at one end and a magnet at the other end. The mounting bracket is provided with an enrichment tube mounting plate, which is made of ferrous metal.

7. The multi-channel fully automated microbial enrichment instrument according to claim 1, characterized in that: The eluent storage tank is connected to a multi-way valve, and a water pump is installed inside the eluent storage tank.

8. The multi-channel fully automated microbial enrichment instrument according to claim 1, characterized in that: The mounting frame is also equipped with a pressure regulator. The side wall of the mounting frame is equipped with a sample tube connector that is connected to the sample water bottle. The sample tube connector is connected to the pressure regulator. The pressure regulator is equipped with an air inlet and a liquid outlet. The enrichment tube is connected to the liquid outlet.

9. The multi-channel fully automated microbial enrichment instrument according to claim 1, characterized in that: The mounting bracket is equipped with a PCB board, the PCB board is equipped with signal wiring terminals, and the mounting bracket is equipped with a connection board connected to the PCB board. The connection board is equipped with a USB interface, an enrichment socket and a power socket.

10. The multi-channel fully automated microbial enrichment instrument according to claim 1, characterized in that: The mounting bracket is also equipped with an ultraviolet disinfection lamp.

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