Cell sampler

Abstract

The utility model discloses a cell sampler which is characterized in that N sampling containers are arranged in parallel, one end of each sampling container is connected with a first joint, and the other end of each sampling container is connected with a filter; the first connector is used for being connected with an external cell container or a pipeline to be sampled, and the filter is used for filtering air entering the cell sampler and balancing internal and external air pressures of the cell sampler; when the cell sap sampling device is used, cell sap in an external cell container flows into the sampling container, and then the two ends of the sampling container are subjected to heat sealing by adopting the heat sealing device, so that one-time sampling of the cell sap can be completed; therefore, the N sampling containers share one first joint and one filter, so that the manufacturing cost is reduced, and repeated sampling can be realized.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, specifically to a cell sampler. Background Technology

[0002] Because the production process of cell-based drugs is characterized by multiple preparation steps and complex processes, the complex process operations and the cell in vitro environment have a significant impact on cell biological activity. Therefore, it is necessary to regularly sample cells during the production process of cell-based drugs to monitor indicators such as cell activity, loss rate, and density.

[0003] In the prior art, sampling is usually carried out using a sampler and sampling device such as the one disclosed in Chinese Patent No. CN218444630U, but each sampling container of the cell solution sampler is connected to a corresponding filter, which results in high manufacturing costs. Utility Model Content

[0004] To overcome the shortcomings of the existing technology, this utility model provides a cell sampler with a simple structure and low cost.

[0005] The first technical solution adopted by this utility model to solve its technical problem is:

[0006] A cell sampler includes: a first connector, a sampling container, and a filter;

[0007] The sampling containers are N in number and connected in parallel;

[0008] All of the sampling containers are connected to the first connector at one end and to the filter at the other end;

[0009] Where N > 1 and is an integer.

[0010] The cell sampler described above also includes N first sub-channels, N first tube clamps, and N second sub-channels;

[0011] Each sampling container is connected to the first sub-pipeline and the second sub-pipeline in a one-to-one correspondence;

[0012] The first pipe clamp is clamped onto the first sub-pipe;

[0013] N first sub-pipes converge and connect to the first connector, and N second sub-pipes converge and connect to the filter.

[0014] The cell sampler described above also includes N second tube clamps, which are clamped onto the second sub-tube.

[0015] The cell sampler described above also includes a first parent conduit and a second parent conduit;

[0016] The end of the first sub-pipe away from the sampling container is connected to the first parent pipe, and the first connector is located at the end of the first parent pipe away from the first sub-pipe.

[0017] The end of the second sub-pipe away from the sampling container is connected to the second parent pipe, and the filter is located at the end of the second parent pipe away from the second sub-pipe.

[0018] As described above, the cell sampler has the following configuration: the first parent conduit includes a first main conduit and a first branch conduit; the second parent conduit includes a second main conduit and a second branch conduit.

[0019] The number of the first branch pipe and the second branch pipe is less than or equal to N;

[0020] The number of the first main pipelines is less than the number of the first branch pipelines, and the number of the second main pipelines is less than the number of the second branch pipelines;

[0021] One end of the first branch pipeline is connected to m first sub-pipelines, and the other end is connected to the first main pipeline;

[0022] One end of the second branch pipeline is connected to the second sub-pipeline m, and the other end is connected to the second main pipeline;

[0023] Where 0 < m ≤ N, and m is an integer.

[0024] As described above, the cell sampler has a spacer inside the sampling container; one end of the spacer is fixedly connected to the inner wall of the sampling container, and the other end is suspended inside the sampling container; the end of the spacer fixedly connected to the sampling container is located between the connection point of the first sub-tube and the sampling container, and the connection point of the second sub-tube and the sampling container.

[0025] The cell sampler described above has graduation lines on the sampling container.

[0026] As described above, the filter of the cell sampler is connected to an external actuator.

[0027] As described above, the cell sampler has a filter connected to an external pump.

[0028] As described above, in the cell sampler, the end of the filter furthest from the second parent conduit is removably covered with a second protective cap.

[0029] The beneficial effects of this utility model are:

[0030] The sampling containers are N in number and connected in parallel. All the sampling containers are connected to the first connector at one end and to the filter at the other end. The first connector is used to connect to an external cell container or a tube to be sampled. The filter is used to filter the air entering the cell sampler and balance the air pressure inside and outside the cell sampler.

[0031] In use, the cell fluid in the external cell container flows into the sampling container, and then the two ends of the sampling container are heat-sealed by a heat sealer to complete one sampling of the cell fluid.

[0032] Therefore, N sampling containers share a single first connector and a single filter, reducing manufacturing costs and enabling multiple, repeated sampling. Attached Figure Description

[0033] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0034] Figure 1 This is one of the connection diagrams of one type of cell sampler in this embodiment;

[0035] Figure 2 This is the second schematic diagram of the connection relationship of one of the cell samplers in this embodiment;

[0036] Figure 3 This is one of the schematic diagrams showing the usage status of the sampling container, the first sub-pipeline, the second sub-pipeline, the first pipe clamp, and the second pipe clamp in this embodiment;

[0037] Figure 4 This is the second schematic diagram showing the usage status of the sampling container, the first sub-pipeline, the second sub-pipeline, the first pipe clamp, and the second pipe clamp in this embodiment;

[0038] Figure 5 This is a schematic diagram of the sampling container, the first sub-pipeline, the second sub-pipeline, and the first pipe clamp in this embodiment;

[0039] The attached figures are labeled as follows:

[0040] 1-First connector; 2-First parent pipeline; 21-First main pipeline; 22-First branch pipeline; 221-Third clamp; 3-First sub-pipeline; 4-First clamp; 5-Sampling container; 6-Second sub-pipeline; 61-Second clamp; 7-Second parent pipeline; 71-Second main pipeline; 72-Second branch pipeline; 8-Filter. Detailed Implementation

[0041] The following will clearly and completely describe the concept, specific structure, and technical effects of this utility model in conjunction with embodiments and accompanying drawings, so as to fully understand the purpose, features, and effects of this utility model. Obviously, the described embodiments are only a part of the embodiments of this utility model, not all of them. Other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are all within the scope of protection of this utility model. Furthermore, all connections / linkages involved in the patent do not simply refer to direct contact between components, but rather to the ability to form a better connection structure by adding or reducing connecting accessories according to specific implementation conditions. The various technical features in this utility model can be combined interactively without contradicting each other.

[0042] Reference Figure 1 , Figure 2 A cell sampler includes: a first connector 1, a sampling container 5, and a filter 8;

[0043] The sampling containers 5 are N in number and connected in parallel;

[0044] All of the sampling containers 5 are connected to the first connector 1 at one end and to the filter 8 at the other end; the first connector 1 is used to connect to an external cell container or a tube to be sampled, and the filter 8 is used to filter the air entering the cell sampler and balance the internal and external air pressure of the cell sampler.

[0045] Where N > 1 and is an integer.

[0046] Reference Figure 1 As an example, N can be a natural integer such as 2, 3, 4, 5, 6, 7, etc.; when N is 3, the three sampling containers 5 are connected in parallel. Therefore, the N sampling containers 5 share a first connector 1 and a filter 8. Compared with the prior art, where one sampling container 5 is connected to one first connector 1 and one filter 8, the cell sampler provided in this embodiment reduces the manufacturing cost of the filter 8 and enables multiple, repeated sampling.

[0047] In one embodiment, it also includes N first sub-pipelines 3, N first pipe clamps 4, and N second sub-pipelines 6;

[0048] Each sampling container 5 is connected to the first sub-pipeline 3 and the second sub-pipeline 6 in a one-to-one correspondence.

[0049] The first pipe clamp 4 is clamped onto the first sub-pipe 3;

[0050] N first sub-pipes 3 converge and connect to the first connector 1, and N second sub-pipes 6 converge and connect to the filter 8.

[0051] When the cell solution is to be loaded into the sampling container 5, the first clamp 4 is opened, and the cell solution flows into the sampling container 5 along the first connector 1 and the first sub-tube 3. When the cell solution volume in the sampling container 5 reaches the predetermined value, the first clamp 4 is used to clamp the first sub-tube 3 to prevent the cell solution from continuing to enter the sampling container 5 and affecting the sampling volume.

[0052] Furthermore, it also includes N second tube clamps 61, which are clamped onto the second sub-tube 6. Before heat sealing the first sub-tube 3 and the second sub-tube 6, the second tube clamps 61 are used to clamp the second sub-tube 6. In this way, during heat sealing, it is not necessary to stabilize the cell sampler, and there is no need to worry about excess cell fluid entering the sampling container 5 or cell fluid flowing out of the sampling container 5 during the heat sealing process.

[0053] Specifically, it also includes the first parent pipe 2 and the second parent pipe 7;

[0054] The end of the first sub-pipeline 3 away from the sampling container 5 is connected to the first parent pipeline 2, and the first connector 1 is located at the end of the first parent pipeline 2 away from the first sub-pipeline 3.

[0055] The end of the second sub-pipeline 6 away from the sampling container 5 is connected to the second parent pipeline 7, and the filter 8 is located at the end of the second parent pipeline 7 away from the second sub-pipeline 6.

[0056] As an example, please refer to Figure 1 A first parent pipe 2 connects to three first child pipes 3. Each first child pipe 3 is connected to a corresponding sampling container 5. Each sampling container 5 is connected to a corresponding second child pipe 6. There are a total of three second child pipes 6. All three second child pipes 6 converge and connect to a second parent pipe 7.

[0057] The usage instructions for this cell sampler are as follows:

[0058] Method 1: Place the first connector 1 in the cell container or the tubing to be sampled, connect the syringe or pump to the filter 8, open the first clamp 4 on one of the first sub-tubings 3, and the syringe or pump draws liquid. The cell fluid in the external cell container flows sequentially along the first connector 1, the first parent tubing 2, and the first sub-tubing 3 into the sampling container 5 (the first clamp 4 clamps the first sub-tubing 3), lift the second sub-tubing 6 (the second clamp 61 clamps the second sub-tubing 6), so that the horizontal position of the sampling container 5 is lower than that of the second sub-tubing 6. Then, use a heat sealer to heat seal the first sub-tubing 3 and the second sub-tubing 6 to complete one sampling of the cells.

[0059] The second method: Place the cell sampler with the first connector 1 on top and the filter 8 on the bottom, open the first clamp on one of the first sub-tubes 3, and position the corresponding sampling container 5 as shown in the image. Figure 3 , Figure 4 Under the influence of gravity, the cell fluid in the external cell container or the tube to be sampled flows sequentially along the first connector 1, the first parent tube 2, and the first daughter tube 3 into the sampling container 5. (The first tube clamp 4 clamps the first daughter tube 3, and the second tube clamp 61 clamps the second daughter tube 6.) Then, the first daughter tube 3 (heat sealing the dotted line A1 or A2) and the second daughter tube 6 (heat sealing the dotted line B1 or B2) are heat sealed with a heat sealer, thus completing one sampling of the cells.

[0060] Whether to use the first clamp 4 and the second clamp 61 during use depends on the volume of cell fluid in the cell container 5 and the operation method of the cell sampler. The operator can also choose according to their needs.

[0061] As can be seen, in this embodiment, only one first connector 1 and one filter 8 are set, which reduces the manufacturing cost and can meet the sampling requirements, enabling multiple and repeated sampling.

[0062] It is understandable that the first parent pipe 2, the first child pipe 3, the second child pipe 6, and the second parent pipe 7 are all flexible hoses.

[0063] Reference Figure 2 In one embodiment, the first parent pipeline 2 includes a first main pipeline 21 and a first branch pipeline 22; the second parent pipeline 7 includes a second main pipeline 71 and a second branch pipeline 72.

[0064] The number of the first branch pipe 22 and the second branch pipe 72 is less than or equal to N;

[0065] The number of first main pipelines 21 is less than the number of first branch pipelines 22, and the number of second main pipelines 71 is less than the number of second branch pipelines 72;

[0066] One end of the first branch pipeline 22 is connected to m first sub-pipelines 3, and the other end is connected to the first main pipeline 21;

[0067] One end of the second branch pipeline 72 is connected to the second sub-pipeline 6, and the other end is connected to the second main pipeline 71;

[0068] Where 0 < m ≤ N, and m is an integer.

[0069] Reference Figure 2 , Figure 3As an example, N is 8 and m is 3; a first main pipe 21 connects to three first branch pipes 22, each first branch pipe 22 connects to two or three first sub-pipes 3, each sub-pipe 3 is connected to a corresponding sampling container 5, each sampling container 5 is connected to a corresponding second branch pipe 72, two or three second branch pipes 72 converge to a second main pipe 71, and three second main pipes 71 converge to a second parent pipe 7. The tubing of the cell sampler is arranged in a tree-like structure, with sampling containers 5 connected in parallel. A first connector 1 and a filter 8 can connect to more sampling containers 5, further reducing manufacturing costs and increasing the number of repeated samplings.

[0070] Furthermore, each of the first branch pipes 22 is provided with a third pipe clamp 221, which is used to clamp the corresponding first branch pipe 22 to prevent cell fluid from flowing into other sampling containers 5.

[0071] In one embodiment, the sampling container 5 is provided with scale lines (not shown in the figure), which are used to measure the volume of cell fluid in the sampling container 5.

[0072] Reference Figure 5 In any of the above embodiments, the sampling container 5 is provided with a spacer 51; one end of the spacer 51 is fixedly connected to the inner wall of the sampling container 5, and the other end is suspended in the sampling container 5; the end of the spacer 51 fixedly connected to the sampling container 5 is located between the connection point of the first sub-pipeline 3 and the sampling container 5, and the connection point of the second sub-pipeline 6 and the sampling container 5.

[0073] In use, the connection points of the first sub-tube 3 and the sampling container 5, and the connection points of the second sub-tube 6 and the sampling container 5 are located on top, with the spacer 51 placed below. The spacer 51 divides the sampling container 5 into two parts. Referring to the second usage method, under the action of gravity, the cell fluid flows into the sampling container 5 along the first sub-tube 3. Since the second sub-tube 6 is at a higher horizontal position and has the spacer 51, the cell fluid will not flow directly out of the sampling container 5 through the second sub-tube 6.

[0074] In the above embodiments, a difference in cell fluid level may occur on both sides of the spacer 51, affecting the measurement of the cell fluid level. To solve this problem, the filter 8 is specifically connected to an external thruster. When a height difference occurs on both sides of the spacer 51, the external thruster is pushed, and under the action of air pressure, the liquid level between the second sub-pipe 6 and the spacer 51 drops. The cell fluid on the other side of the spacer 51 flows back into the first sub-pipe 3. After the backflow is complete, the external thruster is removed. Under the balanced action of air pressure and gravity, the liquid levels on both sides of the spacer 51 are the same, allowing for accurate measurement of the cell fluid volume in the sampling container 5.

[0075] When applied in the above embodiments, when the liquid level in the sampling container 5 exceeds the required sampling capacity, that is, when it exceeds the scale line on the sampling container, an external pump is connected to the filter 8. The external pump can push the liquid level back and return some of the cell fluid, thereby reaching the required sampling capacity and bringing the liquid level to the scale line corresponding to the required sampling. This reduces the waste of cell fluid and improves the utilization rate of cell fluid.

[0076] As an example, the external propulsion device is a syringe.

[0077] In one embodiment, the filter 8 is connected to an external pump.

[0078] As an example, the external pump is a syringe, which is used in the first method of use of this structure.

[0079] In one embodiment, the filter 8 is a sterile filter membrane (not shown in the figure), through which gas can achieve a sterilization effect, in order to ensure that the sampling container 5 is in a sterile environment and to ensure that the cell solution is not contaminated.

[0080] In one embodiment, the filter 8 includes a mounting shell with through holes and a cell-resistant solution filter membrane (not shown) and a sterile filter membrane (not shown) spaced apart within the mounting shell; the cell-resistant solution filter membrane is located between the sterile filter membrane and the sampling container. Understandably, the cell-resistant solution filter membrane is located on the side closer to the sampling container 5, and the sterile filter membrane is located on the side opposite to the sampling container 5. The cell-resistant solution filter membrane and the sterile filter membrane have a certain gap, which is required to prevent the cell-resistant solution filter membrane and the sterile filter membrane from sticking together when cell fluid or gas flows through the through holes.

[0081] In this embodiment, the cell solution filter membrane can act as a barrier to prevent the cell solution from coming into contact with the sterilization filter and causing the sterilization filter to fail. The sterilization filter membrane also prevents impurities in the external gas from entering the sampling container 5, thereby avoiding gas contamination of the cell solution and ensuring the cleanliness of the cell solution.

[0082] It should be noted that the cell-preventing solution filter membrane can be a leukocyte-removing filter membrane, which is capable of filtering leukocytes and preventing leukocytes from flowing out of the pores. The material of the leukocyte-removing filter membrane can be cellulose acetate composite, and the material of the non-woven leukocyte filter material sterilization filter membrane can be polytetrafluoroethylene.

[0083] In one embodiment, the end of the first connector 1 away from the first parent conduit 2 is detachably covered with a first protective cap (not shown in the figure).

[0084] In one embodiment, the end of the filter 8 away from the second parent conduit 7 is detachably covered with a second protective cap (not shown in the figure).

[0085] The first and second protective caps ensure the airtightness of the cell solution sampler. When the cell solution sampler needs to be used to sample the cell solution, the first protective cap and / or the second protective cap are removed, and the first connector 1 is connected to an external cell container or the tubing to be sampled. The filter 8 is suspended in the air, or the filter 8 is connected to an external pump / external pusher.

[0086] Specifically, the first connector 1 is threadedly connected to the first protective cap, and the filter 8 is threadedly connected to the second protective cap.

[0087] Understandably, the first connector 1 includes, but is not limited to, Luer connectors, etc., and the first protective cap includes, but is not limited to, Luer protective caps, etc.; the second protective cap includes, but is not limited to, female Luer connectors, etc.

[0088] The above is a detailed description of the preferred embodiments of the present utility model. However, the present utility model is not limited to the described embodiments. Those skilled in the art can make various equivalent modifications or substitutions without departing from the spirit of the present utility model. All such equivalent modifications or substitutions are included within the scope defined by the claims of this application.

Claims

1. A cell sampler, characterized in that, include: The first connector (1), the sampling container (5), and the filter (8); The sampling containers (5) are N in number and connected in parallel; All of the sampling containers (5) are connected to the first connector (1) at one end and to the filter (8) at the other end; Where N > 1 and is an integer.

2. The cell sampler as described in claim 1, characterized in that: It also includes N first sub-pipelines (3), N first pipe clamps (4), and N second sub-pipelines (6); Each sampling container (5) is connected to the first sub-pipeline (3) and the second sub-pipeline (6) in a one-to-one correspondence; The first pipe clamp (4) is clamped onto the first sub-pipe (3); N first sub-pipes (3) converge to connect to the first connector (1), and N second sub-pipes (6) converge to connect to the filter (8).

3. The cell sampler as described in claim 2, characterized in that: It also includes N second pipe clamps (61), which are clamped on the second sub-pipe (6).

4. The cell sampler as described in claim 2, characterized in that: It also includes the first parent pipeline (2) and the second parent pipeline (7); The end of the first sub-pipe (3) away from the sampling container (5) is connected to the first parent pipe (2), and the first connector (1) is located at the end of the first parent pipe (2) away from the first sub-pipe (3); The end of the second sub-pipe (6) away from the sampling container (5) is connected to the second parent pipe (7), and the filter (8) is located at the end of the second parent pipe (7) away from the second sub-pipe (6).

5. The cell sampler as described in claim 4, characterized in that: The first parent pipeline (2) includes a first main pipeline (21) and a first branch pipeline (22); the second parent pipeline (7) includes a second main pipeline (71) and a second branch pipeline (72); The number of the first branch pipe (22) and the second branch pipe (72) is less than or equal to N; The number of the first main pipeline (21) is less than the number of the first branch pipeline (22), and the number of the second main pipeline (71) is less than the number of the second branch pipeline (72); One end of the first branch pipeline (22) is connected to m first sub-pipelines (3), and the other end is connected to the first main pipeline (21); One end of a second branch pipeline (72) is connected to the second sub-pipeline (6), and the other end is connected to the second main pipeline (71); Where 0 < m ≤ N, and m is an integer.

6. The cell sampler as described in any one of claims 2-5, characterized in that: The sampling container (5) is provided with a spacer (51); one end of the spacer (51) is fixedly connected to the inner wall of the sampling container (5), and the other end is suspended in the sampling container (5); the end of the spacer (51) fixedly connected to the sampling container (5) is located between the connection point of the first sub-pipeline (3) and the sampling container (5), and the connection point of the second sub-pipeline (6) and the sampling container (5).

7. The cell sampler as described in claim 1, characterized in that: The sampling container (5) is equipped with scale lines.

8. The cell sampler as described in claim 7, characterized in that: The filter (8) is connected to an external actuator.

9. The cell sampler as described in claim 1, characterized in that: The filter (8) is connected to an external pump.

10. The cell sampler as described in claim 4, characterized in that: The filter (8) is detachably covered with a second protective cap at the end away from the second parent pipe (7).

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