Solid-phase extraction device for pretreatment of aflatoxin in dairy products

Abstract

The utility model discloses a solid-phase extraction device for pretreatment of aflatoxin in dairy products. The solid-phase extraction device comprises a rack, a solid-phase extraction column, a waste liquid container and a test tube rack, the rack comprises a base, a top plate arranged above the base and a lifting support mechanism for driving the top plate to lift, a plurality of extraction column joints are arranged on the top plate, the upper ends of the extraction column joints are positioned on the upper side of the top plate and are provided with interfaces, and the lower ends of the extraction column joints extend to the lower side of the top plate; the lower end of the solid-phase extraction column is inserted into an interface at the upper end of the extraction column joint; an opening is formed in the upper part of the waste liquid container; a plurality of test tubes are mounted on the test tube rack; wherein in the adsorption process, the waste liquid container is arranged on the base, the lower end of each extraction column connector is connected to the waste liquid container, in the analysis process, the test tube rack is arranged on the base, and the lower end of each extraction column connector is connected to the corresponding test tube. Adsorption and desorption can be completed on one set of equipment, the solid-phase extraction column does not need to be transferred, and the operation is convenient.

Description

Technical Field

[0001] This utility model relates to the field of solid phase extraction technology, specifically to a solid phase extraction device for the pretreatment of aflatoxin in dairy products. Background Technology

[0002] Mycotoxins are secondary metabolites produced by toxin-producing fungi, and are a class of small-molecule substances that are toxic and harmful to plants and animals. Currently, there are 400 known types of mycotoxins, mainly including aflatoxin, deoxynivalenol and its derivatives, fumonisin, zearalenone, and ochratoxin. Mycotoxins are highly carcinogenic, teratogenic, genotoxic, and target organ toxic. Their mechanism involves binding to DNA and RNA in humans and animals, inhibiting the synthesis of proteins and various enzymes, damaging cell structure, impairing the body's immune system, and causing acute or chronic poisoning in humans and animals. This damages the liver, kidneys, nerve tissue, blood tissue, and skin tissue, seriously threatening human and animal health. Among them, aflatoxin M1, the most toxic, is a hydroxyl metabolite of aflatoxin B1 in cow's milk. It is a potent carcinogen and the most prevalent mycotoxin in dairy products, seriously affecting the food safety and quality of dairy products. Mycotoxin residues in dairy products are a key monitoring item for relevant departments, and the development of detection methods has been a research hotspot in various countries.

[0003] The steps for detecting aflatoxin in dairy products are as follows: Weigh 2g of dairy product, add 10mL of methanol-water (70+30) for extraction, vortex mix, centrifuge, take 5mL of the supernatant and add 15mL of water, mix well and then perform solid-phase extraction, and then test the target substance obtained by extraction.

[0004] Solid-phase extraction (SPE) involves two processes: adsorption and desorption. First, the SPE column is inserted into the column connector of the SPE apparatus. After the buffer solution inside the column flows into the waste container, adsorption begins. During adsorption, the entire mixture is passed through the SPE column, and the target analyte is adsorbed onto it. Then, the column is rinsed with 3 mL of water to reduce impurities. The waste liquid generated during this process flows into the waste container below. During desorption, the SPE column is removed and connected to a test tube. Then, 1 mL of methanol is added to elute the target analyte adsorbed on the column. The eluent is collected in the test tube.

[0005] Currently, adsorption and desorption in solid-phase extraction require two sets of equipment and involve transferring the solid-phase extraction column, making the operation quite cumbersome. Utility Model Content

[0006] To address the shortcomings of existing technologies, this invention provides a solid-phase extraction device for the pretreatment of aflatoxin in dairy products, which completes adsorption and desorption in one set of equipment without the need to transfer the solid-phase extraction column, making it easy to operate.

[0007] This invention provides a solid-phase extraction device for the pretreatment of aflatoxin in dairy products, comprising:

[0008] The frame includes a base, a top plate located above the base, and a lifting support mechanism for driving the top plate to rise and fall. The top plate is provided with a plurality of extraction column connectors, the upper end of which is located on the upper side of the top plate and has an interface, and the lower end of which extends to the lower side of the top plate.

[0009] A solid-phase extraction column, wherein the lower end of the solid-phase extraction column is inserted into the interface at the upper end of the extraction column connector;

[0010] Waste liquid container, wherein the waste liquid container has an opening at the top;

[0011] A test tube rack, on which a number of test tubes are mounted;

[0012] During the adsorption process, the waste liquid container is placed on the base, and the lower end of each extraction column connector is connected to the waste liquid container. During the desorption process, the test tube rack is placed on the base, and the lower end of each extraction column connector is connected to the corresponding test tube.

[0013] Furthermore, the base is provided with a positioning groove that is adapted to the lower part of the waste liquid container and the test tube rack.

[0014] Furthermore, the periphery of the positioning groove opening is chamfered.

[0015] Furthermore, the lifting support mechanism includes four lifting columns that are respectively supported between the four corners of the base and the top plate.

[0016] Furthermore, the rising bollard is an electric rising bollard.

[0017] Furthermore, the waste liquid container is provided with a negative pressure interface on its side, which is used to connect to a negative pressure generating device.

[0018] Furthermore, a pressure gauge is externally connected to the negative pressure interface.

[0019] Furthermore, each of the extraction column connectors is equipped with a switching valve.

[0020] The beneficial effects of this utility model are reflected in:

[0021] During adsorption, the waste liquid container is first positioned and installed on the base. The lower end of the solid-phase extraction column is inserted into the interface at the upper end of the extraction column connector. The buffer solution in the solid-phase extraction column flows into the waste liquid tank through the extraction column connector. Then, the solution to be extracted is added to the solid-phase extraction column. The solid-phase extraction column adsorbs the target substance in the solution, and the waste liquid is discharged into the waste liquid container through the extraction column connector. Then, the solid-phase extraction column is rinsed with 3 mL of water, and the waste liquid generated from rinsing also flows into the waste liquid container. During desorption, the top plate is first raised using the lifting support mechanism, and the waste liquid container is removed from the base. Then, the test tube is installed. The test tube rack is positioned on the base and positioned accordingly. Then, the top plate is lowered using the lifting support mechanism, allowing the lower end of the extraction column connector to be inserted into the test tube. 1 mL of methanol is then added to the solid-phase extraction column to elute the target analyte adsorbed on the column. The eluent flows through the extraction column connector and is collected in the test tube. The top plate is then raised using the lifting support mechanism, allowing the lower end of the extraction column connector to exit the test tube. Finally, the test tube rack is removed from the base. Therefore, this application allows adsorption and desorption to be completed on a single device without the need to transfer the solid-phase extraction column, making the operation convenient. Attached Figure Description

[0022] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn to scale.

[0023] Figure 1 This is a schematic diagram of the structure in the adsorption state according to an embodiment of the present invention;

[0024] Figure 2 This is a structural diagram of the present invention in the analytical state of an embodiment;

[0025] Figure 3 This is a schematic diagram of the frame structure of an embodiment of the present utility model;

[0026] Figure 4 This is a schematic diagram of the structure of the waste liquid container according to an embodiment of the present invention;

[0027] Figure 5 This is a schematic diagram of the test tube rack according to an embodiment of the present invention.

[0028] In the attached diagram, 100-frame; 110-base; 111-positioning groove; 112-chamfer; 120-top plate; 121-extraction column connector; 122-switch valve; 130-lifting column; 200-solid phase extraction column; 300-waste liquid container; 310-negative pressure interface; 311-pressure gauge; 400-test tube rack; 410-test tube. Detailed Implementation

[0029] The embodiments of the present invention will now be described in detail with reference to the accompanying drawings. These embodiments are merely illustrative of the present invention and should not be construed as limiting the scope of protection of the present invention.

[0030] It should be noted that, unless otherwise stated, the technical or scientific terms used in this application shall have the ordinary meaning as understood by one of ordinary skill in the art to which this utility model pertains.

[0031] like Figures 1-5 As shown, this utility model embodiment provides a solid phase extraction device for the pretreatment of aflatoxin in dairy products, including a frame 100, a solid phase extraction column 200, a waste liquid container 300, and a test tube rack 400.

[0032] The frame 100 includes a base 110, a top plate 120 disposed above the base 110, and a lifting support mechanism for driving the top plate 120 to rise and fall. The top plate 120 is provided with a plurality of extraction column connectors 121. The upper end of the extraction column connector 121 is located on the upper side of the top plate 120 and is provided with an interface. The lower end of the extraction column connector 121 extends to the lower side of the top plate 120.

[0033] In some embodiments, the lifting support mechanism includes four lifting columns 130 that are respectively supported between the four corners of the base 110 and the top plate 120. The lifting columns 130 are preferably electric lifting columns 130, so that the top plate 120 can be lifted and lowered by controlling the synchronous lifting of each lifting column 130.

[0034] The lower end of the solid phase extraction column 200 is inserted into the interface at the upper end of the extraction column connector 121.

[0035] It should be noted that the solid phase extraction column 200 uses an immunoaffinity column, which works by adsorbing aflatoxin through immunoaffinity. This is a product already available on the market.

[0036] The waste liquid container 300 has an opening at the top, and the waste liquid container 300 can be a glass tank.

[0037] A test tube rack 400 is equipped with several test tubes 410.

[0038] During the adsorption process, the waste liquid container 300 is placed on the base 110, and the lower end of each extraction column connector 121 is connected to the waste liquid container 300. During the desorption process, the test tube rack 400 is placed on the base 110, and the lower end of each extraction column connector 121 is connected to the corresponding test tube 410.

[0039] During adsorption, the waste liquid container 300 is first positioned and installed on the base 110. The lower end of the solid-phase extraction column 200 is inserted into the interface at the upper end of the extraction column connector 121. The buffer solution in the solid-phase extraction column 200 flows into the waste liquid tank through the extraction column connector 121. Then, the solution to be extracted is added to the solid-phase extraction column 200. The solid-phase extraction column 200 adsorbs the target substance in the solution. The waste liquid is discharged into the waste liquid container 300 through the extraction column connector 121. Then, the solid-phase extraction column 200 is rinsed with 3 mL of water. The waste liquid generated from rinsing also flows into the waste liquid container 300. During desorption, the top plate 120 is first raised using the lifting support mechanism, and the waste liquid container 300 is removed from the base 110. Then, the test tube 410 is installed. The test tube rack 400 is positioned on the base 110 at the corresponding location on the test tube rack 400. Then, the top plate 120 is lowered through the lifting support mechanism, so that the lower end of the extraction column connector 121 is inserted into the test tube 410. Then, 1 mL of methanol is added to the solid phase extraction column 200 to elute the target adsorbed on the solid phase extraction column 200. The eluent flows into and is collected in the test tube 410 through the extraction column connector 121. Then, the top plate 120 is raised through the lifting support mechanism, so that the lower end of the extraction column connector 121 is removed from the test tube 410. Then, the test tube rack 400 can be removed from the base 110. Therefore, adsorption and desorption can be completed in one set of equipment without transferring the solid phase extraction column 200, which is convenient to operate.

[0040] In some embodiments, refer to Figure 3 The base 110 is provided with a positioning groove 111 that is adapted to the lower part of the waste liquid container 300 and the test tube rack 400, which facilitates the positioning of the waste liquid container 300 and the test tube rack 400 during installation.

[0041] Optionally, to facilitate the insertion of the waste liquid container 300 / test tube rack 400 into the positioning groove 111 during installation, the periphery of the groove opening of the positioning groove 111 is provided with a chamfer 112.

[0042] In some embodiments, refer to Figure 1 and Figure 4 The waste liquid container 300 is provided with a negative pressure interface 310 on its side. The negative pressure interface 310 is used to connect to a negative pressure generating device, which can be a vacuum pump. During the adsorption process, the top plate 120 presses on the waste liquid container 300 and seals the opening at the top of the waste liquid container 300. The waste liquid container 300 can be evacuated by the negative pressure generating device to maintain a negative pressure inside the waste liquid container 300. This is beneficial for the liquid to flow through the solid phase extraction column 200, and also facilitates the rapid removal of residual liquid in the solid phase extraction column 200 after rinsing.

[0043] Optionally, a pressure gauge 311 is externally connected to the negative pressure port 310. The pressure gauge 311 is used to monitor the vacuum level inside the waste liquid container 300 during the adsorption process.

[0044] Optionally, each extraction column connector 121 is equipped with a switch valve 122. During solid-phase extraction, the corresponding extraction column connector 121 can be selected to connect the solid-phase extraction column 200 according to actual needs. The switch valve 122 on the unused extraction column connector 121 is closed, which can prevent the used extraction column connector 121 from affecting the vacuum degree in the waste liquid container 300.

[0045] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and not to limit it. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this utility model, and they should all be covered within the scope of the claims and specification of this utility model.

Claims

1. A solid-phase extraction apparatus for the pretreatment of aflatoxin in dairy products, characterized in that, include: The frame includes a base, a top plate located above the base, and a lifting support mechanism for driving the top plate to rise and fall. The top plate is provided with a plurality of extraction column connectors, the upper end of which is located on the upper side of the top plate and has an interface, and the lower end of which extends to the lower side of the top plate. A solid-phase extraction column, wherein the lower end of the solid-phase extraction column is inserted into the interface at the upper end of the extraction column connector; Waste liquid container, wherein the waste liquid container has an opening at the top; A test tube rack, on which a number of test tubes are mounted; During the adsorption process, the waste liquid container is placed on the base, and the lower end of each extraction column connector is connected to the waste liquid container. During the desorption process, the test tube rack is placed on the base, and the lower end of each extraction column connector is connected to the corresponding test tube.

2. The solid-phase extraction apparatus for pretreatment of aflatoxin in dairy products according to claim 1, characterized in that, The base is provided with a positioning groove that is adapted to the lower part of the waste liquid container and the test tube rack.

3. The solid-phase extraction apparatus for pretreatment of aflatoxin in dairy products according to claim 2, characterized in that, The periphery of the positioning groove is chamfered.

4. The solid-phase extraction apparatus for pretreatment of aflatoxin in dairy products according to claim 1, characterized in that, The lifting support mechanism includes four lifting columns that are respectively supported between the four corners of the base and the top plate.

5. The solid-phase extraction apparatus for pretreatment of aflatoxin in dairy products according to claim 4, characterized in that, The bollard is an electrically operated bollard.

6. The solid-phase extraction apparatus for pretreatment of aflatoxin in dairy products according to claim 1, characterized in that, The waste liquid container is provided with a negative pressure interface on its side, which is used to connect to a negative pressure generating device.

7. The solid-phase extraction apparatus for pretreatment of aflatoxin in dairy products according to claim 6, characterized in that, A pressure gauge is connected to the negative pressure interface.

8. The solid-phase extraction apparatus for pretreatment of aflatoxin in dairy products according to claim 6, characterized in that, Each extraction column connector is equipped with a switch valve.

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