Double-layer cell sieve for isolated culture of primary cells
By designing an automated double-layer cell sieve, the problems of multi-layer sieving and rapid disassembly in existing technologies have been solved, achieving efficient sieving and convenient operation for primary cell isolation and culture.
Patent Information
- Application Number
- CN202422253605.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-14
- Publication Date
- 2025-11-14
- Estimated Expiration
- 2034-09-14
AI Technical Summary
Existing cell sieves are difficult to use for multi-layer sieving and automated processing in primary cell isolation and culture, and are not easy to disassemble and assemble quickly, resulting in poor performance.
A double-layer cell sieve was designed, comprising a multi-layer sieve box, a vibration motor, guide blocks, and a spring structure, to achieve automated vibration sieve separation. It can be quickly assembled and disassembled through an electric telescopic rod and a locking block. The sieve and collection tray are made of medical stainless steel.
It achieves multi-layer automated screening, improves screening efficiency and practicality, simplifies the disassembly and assembly process of the device, and enhances the convenience and efficiency of cell separation.
Smart Images
Figure CN223548010U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of primary cell isolation and culture technology, specifically a double-layer cell sieve for primary cell isolation and culture. Background Technology
[0002] Primary cell isolation and culture refers to the first culture of tissues or cells taken from the body. Cell sieves are often used in the process of primary cell isolation and culture. Cell sieves are used for laboratory cell culture, impurity filtration, cell dispersion and sample separation. However, there are still some shortcomings in their specific use.
[0003] Cell sieves used for primary cell isolation and culture are often just single-layer screen structures that require manual vibration screening by the user. They are not easy to meet the needs of multi-layer screening, nor are they easy to automate screening processes. Ordinary vibrating screen structures also have screens that are not easy to disassemble and assemble quickly to complete the screening process. This results in poor device performance and difficulty in fully meeting the usage requirements. Based on this, we propose a novel double-layer cell sieve for primary cell isolation and culture. Utility Model Content
[0004] The purpose of this invention is to provide a double-layer cell sieve for primary cell isolation and culture, so as to solve the problems mentioned in the background art.
[0005] To achieve the above objectives, this utility model provides the following technical solution: a double-layer cell sieve for primary cell isolation and culture, comprising a base and a vibration motor. A multi-layer sieve box is connected above the base. The vibration motor is installed on one side of the bottom of the multi-layer sieve box by screws. Guide blocks, springs, and guide seats are uniformly connected between the multi-layer sieve box and the base. A first porous separation sieve, a second porous separation sieve, and a collection tray are installed sequentially from top to bottom inside the multi-layer sieve box. Assembly slides are welded to both ends of the first porous separation sieve, the second porous separation sieve, and the collection tray. Assembly slide grooves matching the assembly slides are provided on the multi-layer sieve box. Electric telescopic rods are uniformly installed on both sides of the multi-layer sieve box. A drive plate is connected inside the side wall of the multi-layer sieve box at the output end of the electric telescopic rod. Locking blocks are uniformly fixed on the drive plate. Locking holes matching the locking blocks are provided on the assembly slides.
[0006] Preferably, the first porous separator, the second porous separator, and the collection tray are all made of medical-grade stainless steel, and the bottom of the first porous separator and the second porous separator are uniformly provided with sieve holes.
[0007] Preferably, the interior of the side wall of the multi-layer screening box is provided with a reserved cavity that matches the drive plate.
[0008] Preferably, a lifting handle is welded to one side of the first porous separating screen, the second porous separating screen, and the collecting tray to facilitate lifting and operating the first porous separating screen, the second porous separating screen, and the collecting tray.
[0009] Preferably, the guide blocks are sequentially installed at the four corners of the bottom of the multi-layer screening box, and the guide seats are sequentially installed at the four corners of the top of the base, with the guide blocks and guide seats forming a sliding connection.
[0010] Preferably, the spring is connected between the guide block and the guide seat.
[0011] Preferably, both the top of the guide block and the bottom of the guide seat are provided with fixing blocks.
[0012] Preferably, the multi-layer screening box and the base are both connected to the fixing block by a snap-fit connection, and screws are provided between the multi-layer screening box and the base and the fixing block, so that the elastic guiding structure composed of spring, guide block and guide seat can be independently disassembled and replaced.
[0013] Compared with the prior art, the beneficial effects of this utility model are:
[0014] (1) The double-layer cell sieve for primary cell isolation and culture is equipped with a second multi-porous separation sieve, which optimizes the performance of the device. On the one hand, the vibration motor installed on one side of the bottom of the multi-layer sieve box is activated. With the elastic action of the spring and the vertical sliding guidance of the guide block and guide seat on the spring, the multi-layer sieve box can be automatically vibrated in the vertical direction. Compared with the common manual vibration sieve, it saves time and effort. On the other hand, by using the first multi-porous separation sieve, the second multi-porous separation sieve and the collection tray arranged in the vertical direction of the multi-layer sieve box, the vibrating multi-layer sieve box drives the first multi-porous separation sieve, the second multi-porous separation sieve and the collection tray to vibrate. Two-layer sieve processing can be achieved. Compared with the common single-layer sieve structure, it is more conducive to sieve different specifications of cell products at one time and enhances practicality.
[0015] (2) The double-layer cell sieve for primary cell separation and culture is equipped with assembly slides, which optimizes the structure of the device. Users can use the sliding connection between the assembly slide and the assembly slide to position and slide the first multi-hole separation sieve, the second multi-hole separation sieve and the collection tray inside the multi-layer sieve box. Subsequently, the electric telescopic rod on the outer wall of the multi-layer sieve box can drive the drive plate to move and realize the locking block on the drive plate to be inserted into or removed from the locking hole on the assembly slide. This facilitates the automatic clamping and fixing or unclamping and fixing of the assembled assembly slide and assembly slide. Compared with the common screw locking structure, it is easier to quickly disassemble and assemble the first multi-hole separation sieve, the second multi-hole separation sieve and the collection tray.
[0016] (3) The double-layer cell sieve for primary cell separation and culture is equipped with fixing blocks, so that when the device is used, the user can use the fixing blocks set on the top of the guide block and the bottom of the guide seat to achieve independent disassembly and replacement of the elastic guide structure composed of spring, guide block and guide seat through the interlocking connection between the multi-layer sieve box and base and the fixing blocks, and the locking and fixing effect of the screws. Attached Figure Description
[0017] Figure 1 This is a front view structural diagram of the present invention;
[0018] Figure 2 This is a partial cross-sectional view of the multi-layer screening box of this utility model.
[0019] Figure 3 This is a schematic diagram of the rear cross-sectional structure of the guide seat of this utility model;
[0020] Figure 4 This is a front view structural diagram of the second porous separator screen of this utility model in its split state;
[0021] Figure 5 This utility model Figure 2 Enlarged structural diagram at point A in the middle.
[0022] In the diagram: 1. Multi-layer screening box; 2. Assembly chute; 3. First multi-hole separating screen; 4. Second multi-hole separating screen; 5. Collection tray; 6. Base; 7. Vibration motor; 8. Guide seat; 9. Electric telescopic rod; 10. Fixing block; 11. Guide block; 12. Spring; 13. Lifting handle; 14. Assembly slide bar; 15. Locking block; 16. Drive plate; 17. Locking hole. Detailed Implementation
[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the scope of protection of the present utility model.
[0024] Please see Figure 1-5 An embodiment of this utility model is provided: a double-layer cell sieve for primary cell isolation and culture, including a base 6 and a vibration motor 7. A multi-layer sieve box 1 is connected above the base 6. The vibration motor 7 is installed on one side of the bottom of the multi-layer sieve box 1 by screws. A guide block 11, a spring 12 and a guide seat 8 are evenly connected between the multi-layer sieve box 1 and the base 6.
[0025] When in use, start the vibration motor 7 installed on one side of the bottom of the multi-layer screening box 1. With the elastic action of the spring 12 and the vertical sliding guidance action of the guide block 11 and guide seat 8 on the spring 12, the multi-layer screening box 1 can achieve automatic vibration in the vertical direction. Compared with the common manual vibration screening, it has the advantages of saving time and effort.
[0026] The multi-layer screening box 1 is equipped with a first porous separation screen 3, a second porous separation screen 4 and a collection plate 5, arranged from top to bottom.
[0027] In use, the first porous separation sieve 3, the second porous separation sieve 4, and the collection tray 5, which are arranged vertically in the multi-layer sieve box 1, are used to drive the first porous separation sieve 3, the second porous separation sieve 4, and the collection tray 5 to vibrate through the vibrating multi-layer sieve box 1. This allows for two-layer sieve processing, which is more advantageous than the common single-layer sieve structure for sieving out cell products of different specifications in one go and enhances practicality.
[0028] The first multi-hole separating screen 3, the second multi-hole separating screen 4, and the collection tray 5 are all welded with assembly slide bars 14 at both ends. The multi-layer screening box 1 is provided with an assembly slide groove 2 that matches the assembly slide bar 14. Electric telescopic rods 9 are evenly installed on both sides of the multi-layer screening box 1. The output end of the electric telescopic rod 9 is connected to the inside of the side wall of the multi-layer screening box 1 with a drive plate 16. Locking blocks 15 are evenly fixed on the drive plate 16. Locking holes 17 that match the locking blocks 15 are provided on the assembly slide bar 14.
[0029] In use, the user can utilize the sliding connection between the assembly slide bar 14 and the assembly slide groove 2 to position and slide the first multi-hole separating screen 3, the second multi-hole separating screen 4, and the collection tray 5 inside the multi-layer screening box 1. Subsequently, activating the electric telescopic rod 9 on the outer wall of the multi-layer screening box 1 can drive the drive plate 16 to move, allowing the locking block 15 on the drive plate 16 to be inserted into or removed from the locking hole 17 on the assembly slide bar 14. This facilitates the automatic clamping and fixing or the release of clamping and fixing of the assembled assembly slide bar 14 and the assembly slide groove 2. Compared with the common screw locking structure, this facilitates quick assembly and disassembly of the first multi-hole separating screen 3, the second multi-hole separating screen 4, and the collection tray 5.
[0030] The first porous separator sieve 3, the second porous separator sieve 4, and the collection tray 5 are all made of medical stainless steel. The bottom of the first porous separator sieve 3 and the second porous separator sieve 4 are uniformly provided with sieve holes.
[0031] The interior of the side wall of the multi-layer screening box 1 is provided with a reserved cavity that matches the drive plate 16;
[0032] A lifting handle 13 is welded to one side of the first porous separator 3, the second porous separator 4, and the collection tray 5 to facilitate lifting and operating the first porous separator 3, the second porous separator 4, and the collection tray 5.
[0033] Guide blocks 11 are installed sequentially at the four corners of the bottom of the multi-layer screening box 1, and guide seats 8 are installed sequentially at the four corners of the top of the base 6. A sliding connection is formed between guide blocks 11 and guide seats 8.
[0034] Spring 12 is connected between guide block 11 and guide seat 8;
[0035] Both the top of the guide block 11 and the bottom of the guide seat 8 are provided with fixing blocks 10;
[0036] Both the multi-layer screening box 1 and the base 6 are engaged with the fixing block 10, and screws are provided between the multi-layer screening box 1 and the base 6 and the fixing block 10.
[0037] In use, the user can use the fixing blocks 10 provided on the top of the guide block 11 and the bottom of the guide seat 8 to achieve independent disassembly and replacement of the elastic guide structure composed of the spring 12, guide block 11 and guide seat 8 through the interlocking connection between the multi-layer screening box body 1 and the base 6 and the fixing blocks 10, and the locking action of the screws.
[0038] In this embodiment, when in use: With an external power supply, the user pours the primary cell product to be processed into the interior of the first porous separation sieve 3. During actual operation, on one hand, the vibration motor 7 installed on one side of the bottom of the multi-layer sieve box 1 is activated. Combined with the elastic action of the spring 12 and the vertical sliding guidance effect of the guide block 11 and guide seat 8 on the spring 12, the multi-layer sieve box 1 can automatically vibrate in the vertical direction. Compared to common manual vibration sieving, this achieves the advantages of saving time and effort. On the other hand, utilizing the first porous separation sieve 3, the second porous separation sieve 4, and the collection tray 5 arranged vertically in the multi-layer sieve box 1, the vibrating multi-layer sieve box 1 drives the first porous separation sieve 3, the second porous separation sieve 4, and the collection tray 5 to vibrate accordingly, achieving two-layer sieving processing. Compared to common single-layer sieve structures, this facilitates the simultaneous sieving of cell products of different specifications, enhancing practicality. Simultaneously, the user can utilize the mounting slide 14 and mounting slide... The sliding connection between the grooves 2 allows the first multi-hole separating screen 3, the second multi-hole separating screen 4, and the collection tray 5 to be positioned and slidably assembled inside the multi-layer screening box 1. Subsequently, activating the electric telescopic rod 9 on the outer wall of the multi-layer screening box 1 can drive the drive plate 16 to move, allowing the locking block 15 on the drive plate 16 to be inserted into or removed from the locking hole 17 on the assembly slide 14. This facilitates the automatic clamping and fixing or release of the assembled assembly slide 14 and assembly groove 2. Compared with the common screw locking structure, this facilitates quick assembly and disassembly of the first multi-hole separating screen 3, the second multi-hole separating screen 4, and the collection tray 5. In addition, the user can use the fixing blocks 10 provided on the top of the guide block 11 and the bottom of the guide seat 8 to achieve independent disassembly and replacement of the elastic guide structure composed of the spring 12, the guide block 11, and the guide seat 8 through the interlocking connection between the multi-layer screening box 1 and the base 6 and the fixing blocks 10, combined with the locking action of the screws.
Claims
1. A double-layer cell sieve for primary cell isolation and culture, characterized in that, Includes a base (6) and a vibration motor (7). A multi-layer screening box (1) is connected above the base (6). The vibration motor (7) is installed on one side of the bottom of the multi-layer screening box (1) by screws. Guide blocks (11), springs (12) and guide seats (8) are evenly connected between the multi-layer screening box (1) and the base (6). The interior of the multi-layer screening box (1) is installed from top to bottom with a first multi-hole separating screen (3), a second multi-hole separating screen (4) and a collection tray (5). The first multi-hole separating screen (3) and the second multi-hole separating screen... (4) Both ends of the collection tray (5) are welded with assembly slides (14). The multi-layer screening box (1) is provided with an assembly slide groove (2) that matches the assembly slide (14). Electric telescopic rods (9) are evenly installed on both sides of the multi-layer screening box (1). The output end of the electric telescopic rod (9) is connected to the inside of the side wall of the multi-layer screening box (1). Locking blocks (15) are evenly fixed on the driving plate (16). Locking holes (17) that match the locking blocks (15) are provided on the assembly slide (14).
2. The double-layer cell sieve for primary cell isolation and culture according to claim 1, characterized in that: The first porous separator (3), the second porous separator (4) and the collection tray (5) are all made of medical stainless steel. The bottom of the first porous separator (3) and the second porous separator (4) are uniformly provided with sieve holes.
3. The double-layer cell sieve for primary cell isolation and culture according to claim 1, characterized in that: The multi-layer screening box (1) has a reserved cavity inside the side wall that matches the drive plate (16).
4. A double-layer cell sieve for primary cell isolation and culture according to claim 1, characterized in that: A lifting handle (13) is welded to one side of the first porous separator (3), the second porous separator (4), and the collection tray (5).
5. A double-layer cell sieve for primary cell isolation and culture according to claim 1, characterized in that: The guide blocks (11) are installed sequentially at the four corners of the bottom of the multi-layer screening box (1), and the guide seats (8) are installed sequentially at the four corners of the top of the base (6). The guide blocks (11) and the guide seats (8) form a sliding connection.
6. A double-layer cell sieve for primary cell isolation and culture according to claim 1, characterized in that: The spring (12) is connected between the guide block (11) and the guide seat (8).
7. A double-layer cell sieve for primary cell isolation and culture according to claim 1, characterized in that: The top of the guide block (11) and the bottom of the guide seat (8) are both provided with fixing blocks (10).
8. A double-layer cell sieve for primary cell isolation and culture according to claim 7, characterized in that: The multi-layer screening box (1) and the base (6) are both connected to the fixing block (10) by a snap-fit connection, and screws are provided between the multi-layer screening box (1) and the base (6) and the fixing block (10).