Tissue culture bottle with scales

By designing graduation marks and fixing components on the tissue culture bottle, the inconvenience of controlling the amount of culture medium and measuring growth indicators in the tissue culture bottle is solved, enabling precise inversion of the culture medium and safe removal of the plant, thus enhancing the use value of the tissue culture bottle.

CN223553983UActive Publication Date: 2025-11-18ANQIU CHANGSHENGYUAN ECOLOGICAL FAMILY FARM
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Patent Information

Application Number
CN202423145686.1
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-12-19
Publication Date
2025-11-18
Estimated Expiration
2034-12-19

AI Technical Summary

Technical Problem

The existing tissue culture bottles lack graduations, making it difficult to accurately control the amount of culture medium and measure growth indicators. Furthermore, the operation is inconvenient when taking off the plants, and it is easy to damage the integrity of the plants.

Method used

A graduated tissue culture bottle was designed with volume and length graduations engraved on its body and equipped with fixing components, including a mounting ring, a limiting block, a limiting ring, and a plate. The cooperation of these components enables convenient installation and sealing of the bottle, ensuring that the culture medium does not leak and facilitating the safe removal of the plants.

Benefits of technology

It enables precise control of culture medium quantity and convenient measurement of growth indicators, while reducing damage during plant removal and improving the ease of use and practicality of tissue culture bottles.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses a scale tissue culture bottle which comprises a base, a bottle body is arranged at the upper end of the base, a bottle cap is arranged at the upper end of the bottle body, scale marks are engraved on the outer wall of the bottle body, first scales are arranged on the left side of the scale marks, second scales are arranged on the right side of the scale marks, and a fixing assembly used for installing the bottle body is arranged on the outer wall of the base. The utility model relates to the technical field of biology, and is provided with the fixing component, the mounting ring and the blocking block are convenient to separate, the inserting plate can be pulled out only by rotating and taking down the mounting sleeve, and the bottle body can be separated from the base by rotating the bottle body clockwise with force, so that plants in the tissue culture bottle can be taken out conveniently; the graduated tissue culture bottle can prevent the integrity of plants from being damaged to a certain extent, is higher in use value and more convenient to use, and facilitates the guarantee of the integrity of the plants.
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Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biotechnology, specifically is scale tissue culture bottle. BACKGROUND

[0002] Plant tissue culture technology has been widely applied to the field of agricultural biotechnology, in the plant tissue culture process, researchers complete the culture and propagation work of plant seedling in the sterile culture container, realize the regeneration of plant seedling; tissue culture bottle is the culture container used in the technology that makes plants grow and develop under artificial conditions through aseptic operation method.

[0003] In the tissue culture process, the tissue culture bottle is an indispensable vessel, the tissue culture bottle mainly has glass and plastic products two kinds, the most common has triangle bottle, along with the development of culture technology, the tissue culture bottle also realizes diversification, although the existing tissue culture container has carried out various improvements, but the culture bottle is mostly composed of bottle body and cover body with hollow structure, the culture medium is filled in the bottle body during use, the tissue culture bottle with the structure is inconvenient to use, when the plant in the tissue culture bottle needs to be taken out, the bottle cover is opened from the top of the tissue culture bottle to take the plant, since the plant is inverted triangular shape, this taking mode is inconvenient to operate, and the integrity of the plant can be damaged, and the existing tissue culture bottle does not have a scale, on the one hand, it is difficult to accurately control the amount when pouring the culture medium, on the other hand, it is inconvenient to measure growth indexes such as plant height and root length, therefore, the existing tissue culture bottle has limitations in use. UTILITY MODEL CONTENTS

[0004] The utility model aims at providing scale tissue culture bottle to solve the problems in the above background technology.

[0005] To achieve the above object, the utility model provides the following technical scheme:

[0006] The scale tissue culture bottle comprises a base, a bottle body is arranged at the upper end of the base, a bottle cover is arranged at the upper end of the bottle body, a scale line is engraved on the outer wall of the bottle body, a first scale is arranged at the left side of the scale line, a second scale is arranged at the right side of the scale line, and a fixing assembly for mounting the bottle body is arranged on the outer wall of the base.

[0007] In one preferred embodiment of the utility model, the unit of the first scale is milliliter, the minimum scale value of the first scale is 5 milliliters, the first scale is marked from 0 milliliter to 325 milliliters, and the maximum capacity of the bottle body is 350 ml.

[0008] In one preferred embodiment of the utility model, the second scale is a length scale, the minimum scale value of the second scale is 1 millimeter, and the second scale is marked from 0 centimeter to 10 centimeters.

[0009] In a preferred embodiment of the utility model, the bottle body upper end is provided with a connecting part, the connecting part outer side is provided with a thread, the bottle cap lower end inner wall is provided with a thread matched with the thread provided on the connecting part outer side.

[0010] In a preferred embodiment of the utility model, the fixing assembly comprises a mounting ring, the mounting ring is fixedly installed at the bottle body lower end, and the mounting ring outer wall is fixedly installed with limit blocks on four sides.

[0011] In a preferred embodiment of the utility model, the base upper end is fixedly installed with a plug, the plug outer wall is fixedly installed with a sealing ring, and the base upper end inner wall and located on the plug outer side is provided with a clamping groove.

[0012] In a preferred embodiment of the utility model, the base inner wall four sides is provided with a slot, the slot is an L-shaped slot, the base upper end is provided with a limit ring, and the limit ring lower end four sides is fixedly installed with an insertion plate.

[0013] In a preferred embodiment of the utility model, the limit ring upper end outer wall is provided with a mounting sleeve, the mounting sleeve inner wall is tapped with a thread, the base outer wall is threadedly connected with the mounting sleeve, and the mounting sleeve outer wall is fixedly installed with a baffle.

[0014] The additional aspects and advantages of the utility model will be partially given in the following description, some will become obvious from the following description, or be known by the practice of the utility model.

[0015] Beneficial effects:

[0016] 1. The utility model discloses a capacity scale and length scale are arranged on the bottle body of tissue culture bottle, solve the existing tissue culture bottle without scale, on one hand, it is difficult to accurately control the amount when pouring culture medium, on the other hand, it is inconvenient to measure growth index, the utility model discloses the use value is higher, and the practicality is stronger, makes the amount of pouring culture medium more accurate, and it is convenient to measure growth index.

[0017] 2. The utility model discloses a fixing assembly, through the mutual limiting between mounting ring, limit block, limit ring, insertion plate and mounting sleeve, make the mounting ring of bottle body bottom fixedly installed on the base upper end inner wall, and the interference fit between sealing ring and mounting ring, make the bottle body bottom be in sealed state, can prevent culture medium from leaking, simultaneously, the structure makes the separation between mounting ring and plug convenient, only need to rotate and take down mounting sleeve, can pull out insertion plate, rotate bottle body with force clockwise, can separate bottle body and base, convenient to take out the plant in tissue culture bottle, can prevent the integrity of plant from being damaged to a certain extent, the scale tissue culture bottle use value is higher, and use is more convenient, convenient guarantee the integrity of plant. DRAWINGS

[0018] The above and / or additional aspects and advantages of the present application will become apparent and more readily appreciated from the following description of the embodiments, taken in conjunction with the references to the following drawings, of which:

[0019] Figure 1 It is a stereoscopic structure schematic view of the scale tissue culture bottle.

[0020] Figure 2 It is a structure schematic view of the bottle cap opening state in the scale tissue culture bottle.

[0021] Figure 3 It is a structure schematic view of the fixing assembly in the scale tissue culture bottle.

[0022] Figure 4 It is a structure schematic view of the position relationship between the plug-in plate and the limiting block in the scale tissue culture bottle in the partial section state of the base.

[0023] Figure 5 It is a structure schematic view of the base in the scale tissue culture bottle.

[0024] In the figure: base 1; clamping groove 101; insertion groove 102; plug block 13; sealing ring 14; bottle body 2; connecting part 21; bottle cap 3; first scale 4; second scale 5; mounting ring 6; limiting block 61; limiting ring 7; plug-in plate 71; mounting sleeve 8; baffle 81. DETAILED DESCRIPTION

[0025] The embodiments of the present application will be described in detail below, examples of which are shown in the drawings, wherein the same or similar reference signs represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary and are only used to explain the present application, and cannot be understood as a limitation of the present application.

[0026] Embodiment 1: as Figures 1-5 , including base 1, the upper end of the base 1 is provided with bottle body 2, the upper end of the bottle body 2 is provided with bottle cap 3, the outer wall of the bottle body 2 is engraved with scale line, the left side of the scale line is provided with first scale 4, the right side of the scale line is provided with second scale 5, the outer wall of the base 1 is provided with fixing assembly for installing bottle body 2; the unit of the first scale 4 is milliliter, and the minimum scale value of the first scale 4 is 5 milliliter, from 0 milliliter mark to 325 milliliter, the maximum capacity of the bottle body 2 is 350ml; the second scale 5 is length scale, and the minimum scale value of the second scale 5 is 1 millimeter, the second scale 5 is from 0 centimeter mark to 10 centimeters;

[0027] The specific practical scene of this embodiment is that the first scale 4 represents 5ml per small box and 25ml per large box, which is used for measuring the amount of culture medium; the amount of inverted culture medium is more accurate; the second scale 5 represents 1mm per small box and 1cm per large box, which is used for measuring growth indicators such as plant height and root length; the maximum capacity of the tissue culture bottle is 350ml, and the maximum height is 11cm;

[0028] Embodiment 2: as Figures 1-5 , comprising a base 1, the upper end of the base 1 is provided with a bottle body 2, the upper end of the bottle body 2 is provided with a bottle cap 3, the outer wall of the bottle body 2 is marked with a scale line, the left side of the scale line is provided with a first scale 4, the right side of the scale line is provided with a second scale 5, and the outer wall of the base 1 is provided with a fixing assembly for installing the bottle body 2; the upper end of the bottle body 2 is provided with a connecting part 21, the outer side of the connecting part 21 is provided with a thread, and the inner wall of the lower end of the bottle cap 3 is provided with a thread matched with the thread provided on the outer side of the connecting part 21;

[0029] The specific practical scene of this embodiment is that the bottle cap 3 is threadedly installed on the outer wall of the upper end of the bottle body 2, and the bottle cap 3 adopts the prior art;

[0030] Embodiment 3: as Figures 1-5 , comprising a base 1, the upper end of the base 1 is provided with a bottle body 2, the upper end of the bottle body 2 is provided with a bottle cap 3, the outer wall of the bottle body 2 is marked with a scale line, the left side of the scale line is provided with a first scale 4, the right side of the scale line is provided with a second scale 5, and the outer wall of the base 1 is provided with a fixing assembly for installing the bottle body 2; the fixing assembly comprises a mounting ring 6, the mounting ring 6 is fixedly installed at the lower end of the bottle body 2, and the outer wall of the mounting ring 6 is fixedly installed with a limiting block 61 on four sides;

[0031] The specific practical scene of this embodiment is that the mounting ring 6 and the bottle body 2 are in an integral structure; the mounting ring 6 can be inserted into the clamping groove 101, and the limiting block 61 can be inserted into the insertion groove 102;

[0032] Embodiment 4: as Figures 1-5 , the upper end of the base 1 is fixedly installed with a plug 13, the outer wall of the plug 13 is fixedly installed with a sealing ring 14, and the inner wall of the upper end of the base 1 and located on the outer side of the plug 13 is provided with a clamping groove 101;

[0033] The specific practical scene of this embodiment is that the sealing ring 14 has sealing strips equidistantly distributed on the outer wall, when the mounting ring 6 is inserted into the clamping groove 101, the plug 13 can enter the inner wall of the mounting ring 6, the mounting ring 6 is located between the plug 13 and the base 1, as the plug 13 continuously enters the inner wall of the mounting ring 6, the sealing strips are extruded and deformed to tightly adhere to the inner wall of the mounting ring 6, so that the friction between the sealing ring 14 and the mounting ring 6 is continuously expanded, and the gap between the plug and the mounting ring 6 can be filled by the sealing ring 14 to seal the bottom of the bottle body 2, and the bottle body 2 is rotated to make the limiting block 61 rotate inside the insertion slot 102 and move to one end of the L-shaped insertion slot 102; the top of the plug 13 is flush with the bottom of the bottle body 2;

[0034] Embodiment 5: as Figures 1-5 , the base 1 has insertion slots 102 formed on the inner wall of four sides, the insertion slots 102 are L-shaped slots, the base 1 is provided with a limiting ring 7 at the upper end, and the limiting ring 7 is fixedly installed with insertion plates 71 on the four sides of the lower end;

[0035] The specific practical scene of this embodiment is that the insertion slot 102 is connected with the clamping groove 101, when the limiting block 61 is located at one end of the L-shaped insertion slot 102, the limiting ring 7 can be moved downward, so that the insertion plates 71 are inserted into the insertion slot 102, and the limiting block 61 cannot leave the insertion slot 102 by the limitation of the insertion plates 71, so that the mounting ring 6 cannot be separated from the base 1;

[0036] Embodiment 6: as Figures 1-5 , the limiting ring 7 is provided with a mounting sleeve 8 on the outer wall of the upper end, the mounting sleeve 8 is threaded on the inner wall, the base 1 is threadedly connected with the mounting sleeve 8 on the outer wall, and the mounting sleeve 8 is fixedly installed with a baffle 81 on the outer wall;

[0037] The specific practical scene of this embodiment is that the base 1 is provided with threads on the outer wall matched with the threads on the inner wall of the mounting sleeve 8, so that the mounting sleeve 8 can be threadedly installed on the outer wall of the base 1, when the mounting sleeve 8 is completely installed on the outer wall of the base 1, the inner wall of the mounting sleeve 8 abuts against the upper side of the limiting ring 7, so that the limiting ring 7 cannot be moved upward, at this time, the bottom of the bottle body 2 is in a sealed state, which can prevent the culture medium from leaking, and the bottom of the bottle body 2 and the top of the mounting ring 6 are the starting points of the scale line;

[0038] The above structure and principle are general standard parts or components known to those skilled in the art, and the structure and principle can be known by technical personnel through a technical manual or through a conventional experimental method;

[0039] The utility model discloses a work principle is: when using this tissue culture bottle can through the first scale 4 observation bottle body 2 inside the volume of the medium that pours, through the second scale 5 measure plant growth index, when needing to take out the plant of cultivation, rotate and unscrew the mounting sleeve 8, make the mounting sleeve 8 move up and leave the base 1, move up the limiting ring 7, pull out the plug -in board 71 from the inside of the slot 102, at this moment can rotate bottle body 2, drive the installation ring 6 rotation, make the limiting block 61 move to the just below gap, at this moment move up bottle body 2, the pulling force that applies to bottle body 2 is greater than the friction between sealing washer 14 and installation ring 6, make installation ring 6 can separate with sealing washer 14, can take down bottle body 2, take the plant;

[0040] Although the embodiments of the utility model have been shown and described, those skilled in the art can understand that: can carry out multiple changes, modification, replacement and variation to these embodiments without departing from the principles and purposes of the utility model, the range of the utility model is defined by the claims and its equivalents.

Claims

1. A graduated tissue culture flask, including a base (1), characterized in that, The base (1) is provided with a bottle body (2) at the upper end, and a bottle cap (3) is provided at the upper end of the bottle body (2). The outer wall of the bottle cap (3) is engraved with scale lines. A first scale (4) is provided on the left side of the scale lines, and a second scale (5) is provided on the right side of the scale lines. The outer wall of the base (1) is provided with a fixing component for installing the bottle body (2).

2. The graduated tissue culture bottle according to claim 1, characterized in that, The first scale (4) is in milliliters, and the smallest division of the first scale (4) is 5 milliliters, ranging from 0 milliliters to 325 milliliters. The maximum capacity of the bottle body (2) is 350 milliliters.

3. The graduated tissue culture bottle according to claim 1, characterized in that, The second scale (5) is a length scale, and the minimum division value of the second scale (5) is 1 millimeter. The second scale (5) is marked from 0 centimeters to 10 centimeters.

4. The graduated tissue culture bottle according to claim 1, characterized in that, The upper end of the bottle body (2) is provided with a connecting part (21), and the outer side of the connecting part (21) is provided with a thread. The inner wall of the lower end of the bottle cap (3) is provided with a thread that matches the thread on the outer side of the connecting part (21).

5. The graduated tissue culture bottle according to claim 1, characterized in that, The fixing component includes a mounting ring (6), which is fixedly installed at the lower end of the bottle body (2), and limit blocks (61) are fixedly installed on all four sides of the outer wall of the mounting ring (6).

6. The graduated tissue culture flask according to claim 1, characterized in that, A block (13) is fixedly installed on the upper end of the base (1), and a sealing ring (14) is fixedly installed on the outer wall of the block (13). A slot (101) is opened on the inner wall of the upper end of the base (1) and outside the block (13).

7. The graduated tissue culture flask according to claim 1, characterized in that, The base (1) has slots (102) on its four sides. The slots (102) are L-shaped slots. The upper end of the base (1) is provided with a limit ring (7). The lower end of the limit ring (7) is fixedly installed with insert plates (71) on its four sides.

8. The graduated tissue culture flask according to claim 7, characterized in that, The upper outer wall of the limiting ring (7) is provided with an installation sleeve (8), the inner wall of the installation sleeve (8) is threaded, the outer wall of the base (1) is threaded to the installation sleeve (8), and a baffle (81) is fixedly installed on the outer wall of the installation sleeve (8).