Protein vertical electrophoresis gel preparation tank

Abstract

The utility model discloses a protein vertical electrophoresis gel preparation tank, which relates to the technical field of biological experiments, and comprises a main body gel tank, a thick filling plate, a thin partition plate and a wedge-shaped inserting plate, the main body gel tank comprises a bottom plate, the upper surface of the bottom plate is fixedly connected with two vertical side plates, one ends of the two vertical side plates are fixedly connected with an inclined front plate, and the other ends of the two vertical side plates are fixedly connected with the wedge-shaped inserting plate. The other ends of the two vertical side plates are fixedly connected with a vertical rear plate, and it needs to be explained that the main body glue groove is composed of a bottom plate, the vertical side plates, the vertical rear plate and an inclined front plate. According to the protein vertical electrophoresis gel preparation tank, the integrated main body gel tank design is adopted, the gel leakage condition in the protein gel preparation process is completely eradicated, and the inclined front plate, the wedge-shaped insertion plate, the thick filling plate and the thin partition plate are arranged on the inner wall of the main body gel tank, so that the gel removal process is simple and easy to implement, the gel preparation work efficiency is improved, and the production cost is reduced. And the gel leakage phenomenon in the preparation process of the protein electrophoresis gel is thoroughly avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biological experiment technology, especially to a protein vertical electrophoresis gel preparation tank. BACKGROUND

[0002] Protein vertical electrophoresis is a common test content in biological related laboratories. Protein vertical electrophoresis test needs to use disposable protein electrophoresis gel. Protein electrophoresis gel is usually prepared by the laboratory itself. The mainstream glass mold for preparing protein electrophoresis gel on the market has the same size (Bio-Rad, 1653311, 1653308). The common preparation frame (Bio-Rad, 1653304) and the pouring frame (Bio-Rad, 1653303) are used for pouring gel. However, the pouring frame and the pouring frame often have the phenomenon of gel leakage during the pouring work, which affects the test.

[0003] The protein gel preparation container in the prior art can solve the problem of gel leakage of the pouring frame to a certain extent, but due to the design of the half-enclosed groove, the sealing strip, the front plate and the fastener, there is still a risk of gel leakage at the sealing strip and other places after a period of use, and a single gel leakage event will cause multiple gels to be scrapped at the same time, affecting the efficiency of the gel preparation work. Therefore, we designed a new type of protein vertical electrophoresis gel preparation tank, which completely eliminated the gel leakage phenomenon during the preparation process and provided convenient and reliable support for the preparation of protein electrophoresis gel in the laboratory. UTILITY MODEL CONTENT

[0004] The utility model discloses a protein vertical electrophoresis gel preparation tank, in order to realize above-mentioned purpose, the utility model has adopted following technical scheme:

[0005] A protein vertical electrophoresis gel preparation tank, comprising a main body gel tank, a thick filling plate, a thin partition plate and a wedge-shaped insert plate, the main body gel tank comprises a bottom plate, the upper surface of the bottom plate is fixedly connected with two vertical side plates, one end of the two vertical side plates is fixedly connected with an inclined front plate, and the other end of the two vertical side plates is fixedly connected with a vertical back plate.

[0006] It should be noted that the main body gel tank is composed of a bottom plate, vertical side plates, a vertical back plate and an inclined front plate.

[0007] The inner side surface of the inclined front plate is provided with a lower gel pouring groove, and the top of the vertical side plate is provided with an upper gel pouring groove.

[0008] The thick filling plate, the thin partition plate and the wedge-shaped insert plate are sequentially installed in the inside of the main body gel tank from left to right.

[0009] The inclined front plate, the wedge-shaped insert plate, the thick filling plate and the thin partition plate are arranged on the inner wall of the main glue groove, so that the glue removal process is simple and easy, and the efficiency of glue production is improved.

[0010] In a preferred scheme, the wedge-shaped insert plate is composed of a large wedge-shaped insert plate, a medium wedge-shaped insert plate and a small wedge-shaped insert plate, and the large wedge-shaped insert plate, the medium wedge-shaped insert plate and the small wedge-shaped insert plate are placed on the surface of the inclined front plate in sequence.

[0011] The large, medium and small wedge-shaped insert plates are arranged, so that the wedge-shaped cavity formed by the inclined front plate of the filling groove body is beneficial to the molding of the glue.

[0012] In a preferred scheme, the upper surface of the bottom plate is fixedly connected with two flow guide plates, both of which are fixedly connected with the inner side of the vertical side plate, the left end of the flow guide plate is fixedly connected with the right side of the vertical rear plate, and the right end of the flow guide plate is fixedly connected with the surface of the inclined front plate.

[0013] In a preferred scheme, a flow guide glue groove is arranged between the two flow guide plates, the right end of the flow guide glue groove is communicated with the bottom end of the lower glue pouring glue groove, and the size of the flow guide glue groove is matched with that of the lower glue pouring glue groove.

[0014] The flow guide glue groove is arranged between the flow guide plates, which can provide the protein electrophoresis gel with the lower layer glue pouring glue.

[0015] In a preferred scheme, the outer side of the vertical side plate is fixedly connected with a strip-shaped baffle, and the position of the strip-shaped baffle corresponds to the upper glue pouring glue groove.

[0016] The strip-shaped baffle is arranged on the outer side of the vertical side plate, which can shield the outer side of the upper glue pouring glue groove, so as to facilitate the glue pouring into the upper glue pouring glue groove.

[0017] In a preferred scheme, the upper surfaces of the large, medium and small wedge-shaped insert plates are fixedly connected with limiting strip blocks, and the two ends of the limiting strip blocks are placed on the upper surface of the vertical side plate.

[0018] The limiting strip blocks are arranged, which can limit and support the wedge-shaped insert plate.

[0019] In a preferred scheme, a plurality of protein glue glass plates are arranged between the thin partition plates, and the plurality of protein glue glass plates are distributed between the thick filling plate and the wedge-shaped insert plate in an interlaced manner.

[0020] The device is used in combination with a protein glue glass plate and a protein glue sample comb on the market, the lower gel liquid is poured into the inclined plate pouring glue port, the isopropyl alcohol is poured into the vertical side plate pouring glue groove, after the lower gel solidifies, the isopropyl alcohol is poured out, the upper gel liquid is poured into the vertical side plate pouring glue groove, the protein glue sample comb is inserted, after the upper gel solidifies, the wedge-shaped insertion plate, the protein glue glass plate, the thin partition plate and the thick filling plate are removed in sequence.

[0021] As can be seen from the above, the protein vertical electrophoresis gel preparation groove has the following technical effects.

[0022] Firstly, the protein vertical electrophoresis gel preparation groove adopts an integrated main glue groove design, so that the glue leakage in the protein glue preparation process is avoided, and the glue removal process is simple and easy to operate, the efficiency of glue preparation work is improved, and the glue leakage phenomenon in the protein electrophoresis gel preparation process is completely avoided.

[0023] Secondly, the thin partition plate is used for spacing the glass glue plate mold, the glass glue plate can be protected during the glue removal process, the three wedge-shaped insertion plates are arranged, the wedge-shaped cavities are formed by the inclined filling groove body front plate, the glue body is beneficial to molding, the wedge-shaped insertion plate, the protein glue glass plate, the thin partition plate and the thick filling plate can be removed in sequence, and the glue preparation groove can be repeatedly used after cleaning. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 The main glue groove structure schematic view of the protein vertical electrophoresis gel preparation groove is provided.

[0025] Figure 2 The overall structure schematic view of the protein vertical electrophoresis gel preparation groove is provided.

[0026] Figure 3 The main glue groove local side cross-sectional structure schematic view of the protein vertical electrophoresis gel preparation groove is provided.

[0027] Figure 4 The main glue groove local rear cross-sectional structure schematic view of the protein vertical electrophoresis gel preparation groove is provided.

[0028] Figure 5 The wedge-shaped insertion plate structure schematic view of the protein vertical electrophoresis gel preparation groove is provided.

[0029] In the drawings: 1, main glue groove; 2, thick filling plate; 3, thin partition plate; 4, wedge-shaped insertion plate; 5, lower glue pouring groove; 6, upper glue pouring groove; 7, flow guide plate; 8, glue pouring groove; 9, strip-shaped baffle; 10, protein glue glass plate;

[0030] 101, bottom plate; 102, vertical side plate; 103, inclined front plate; 104, vertical rear plate;

[0031] 401, large wedge-shaped plug-in plate; 402, medium wedge-shaped plug-in plate; 403, small wedge-shaped plug-in plate; 404, limiting strip block. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments.

[0033] In the description of the utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the utility model and simplifying the description, and do not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the utility model.

[0034] Referring to Figure 1 and Figure 2 A protein vertical electrophoresis gel preparation tank, comprising a main body gel tank 1, a thick filling plate 2, a thin partition plate 3 and a wedge-shaped plug-in plate 4, the main body gel tank 1 comprises a bottom plate 101, the upper surface of the bottom plate 101 is fixedly connected with two vertical side plates 102, one end of the two vertical side plates 102 is fixedly connected with an inclined front plate 103, and the other end of the two vertical side plates 102 is fixedly connected with a vertical rear plate 104.

[0035] It is worth noting that the protein vertical electrophoresis gel preparation tank adopts an integrated main body gel tank 1 design, which completely eliminates the glue leakage during the protein glue preparation process, and through the inclined front plate 103, the wedge-shaped plug-in plate 4, the thick filling plate 2 and the thin partition plate 3 arranged on the inner wall of the main body gel tank 1, the glue removal process is simple and easy to operate, which is beneficial to improve the efficiency of the glue preparation work, and completely eliminates the glue leakage phenomenon in the protein electrophoresis gel preparation process.

[0036] Referring to Figure 3 and Figure 4 In a preferred embodiment, a plurality of protein glue glass plates 10 are installed between the plurality of thin partition plates 3, and the plurality of protein glue glass plates 10 and the thin partition plates 3 are staggered and distributed in the area between the thick filling plate 2 and the wedge-shaped plug-in plate 4.

[0037] It is worth noting that the thick filling plate 2 is arranged to fill the glue pouring tank cavity, so that it is suitable for preparing 2 to 16 protein gels at a time.

[0038] By setting the thin partition, 3, the thin partition plate 3 is used to separate the glass glue plate mold, which can protect the protein glue glass plate 10 during the glue removal process.

[0039] It should be noted that the inner side surface of the inclined front plate 103 is provided with a lower layer of glue pouring groove 5, and the top of the vertical side plate 102 is provided with an upper layer of glue pouring groove 6.

[0040] It should be noted that the outer side of the vertical side plate 102 is fixedly connected with a strip-shaped baffle 9, and the position of the strip-shaped baffle 9 corresponds to the upper layer of glue pouring groove 6.

[0041] The thick filler plate 2, the thin partition plate 3 and the wedge-shaped insert plate 4 are installed in the inside of the main body glue groove 1 from left to right.

[0042] Referring to Figure 5 In a preferred embodiment, the wedge-shaped insert plate 4 is composed of a large wedge-shaped insert plate 401, a medium wedge-shaped insert plate 402 and a small wedge-shaped insert plate 403, and the large wedge-shaped insert plate 401, the medium wedge-shaped insert plate 402 and the small wedge-shaped insert plate 403 are placed on the surface of the inclined front plate 103 in order.

[0043] It should be noted that the upper surface of the large wedge-shaped insert plate 401, the medium wedge-shaped insert plate 402 and the small wedge-shaped insert plate 403 is fixedly connected with a limiting strip block 404, and the both ends of the limiting strip block 404 are placed on the upper surface of the vertical side plate 102.

[0044] It is worth noting that by setting the large, medium and small wedge-shaped insert plates 4, the wedge-shaped cavity formed by the inclined filler groove body front plate is beneficial to the molding of the glue.

[0045] In a preferred embodiment, the upper surface of the bottom plate 101 is fixedly connected with two flow guide plates 7, both of which are fixedly connected with the inner side of the vertical side plate 102, the left end of the flow guide plate 7 is fixedly connected with the right side of the vertical rear plate 104, and the right end of the flow guide plate 7 is fixedly connected with the surface of the inclined front plate 103.

[0046] It should be noted that the two flow guide plates 7 are provided with a flow guide glue groove 8, the right end of the flow guide glue groove 8 is communicated with the bottom end of the lower layer of glue pouring groove 5, and the size of the flow guide glue groove 8 matches that of the lower layer of glue pouring groove 5.

[0047] By setting the flow guide glue groove 8 between the flow guide plates 7, the flow guide glue groove 8 can provide the flow of glue liquid when the protein electrophoresis gel lower layer of glue is poured.

[0048] Working principle: First, wash and dry all components of the main gel tank 1 and an appropriate number of commercially available protein gel glass plates. From the vertical rear plate 104 to the inclined front plate 103, place 0 to 8 thick filling plates 2, thin partitions 3, and protein gel glass plates 10 into the tank in sequence, with several thin partitions 3 and several protein gel glass plates 10 spaced apart. Then, place the large wedge-shaped insert 401, the medium wedge-shaped insert 402, and the small wedge-shaped insert 403. Next, prepare a sufficient amount of protein electrophoresis lower layer gel and pour it into the lower layer gel pouring tank 5 on the surface of the inclined front plate 103 of the gel tank until the gel level rises to an appropriate height. Then, pour isopropyl alcohol into the upper layer gel pouring tank 6 on the surface of the vertical side plate 102 of the gel tank to fill the tank. Let it stand for 30 minutes until the lower layer gel solidifies. Tilt the main gel tank 1 and pour out the upper layer of isopropanol into the recycling bottle. The isopropanol can be reused. After the gel tank is drained, prepare a sufficient amount of protein electrophoresis upper gel and pour it into the upper gel filling tank 6 to fill the main gel tank 1. Insert a commercially available protein gel loading comb and let it stand for 30 minutes until the upper gel solidifies. Remove the small wedge plate 403, the medium wedge plate 402, and the large wedge plate 401 in sequence. Remove the protein gel in sequence from the tilted front plate 103 to the vertical rear plate 104. Clean the surface of the protein gel glass plate 10 to remove excess gel. It is ready for use.

[0049] The above description is merely a preferred embodiment of this utility model, but the protection scope of this utility model is not limited thereto. The substitutions may be replacements of some structures, devices, or method steps, or they may be complete technical solutions. Equivalent substitutions or modifications made based on the technical solution and inventive concept of this utility model should all be covered within the protection scope of this utility model.

Claims

1. A vertical electrophoresis gel making tank for protein, comprising a main tank (1), a thick filling plate (2), a thin partition plate (3) and a wedge-shaped insert plate (4), characterized in that, The body glue tank (1) comprises a bottom plate (101), the upper surface of the bottom plate (101) is fixedly connected with two vertical side plates (102), one end of the two vertical side plates (102) is fixedly connected with an inclined front plate (103), and the other end of the two vertical side plates (102) is fixedly connected with a vertical rear plate (104). The inner side surface of the inclined front plate (103) is provided with a lower glue pouring glue tank (5), and the top of the vertical side plate (102) is provided with an upper glue pouring glue tank (6). The thick filling plate (2), the thin partition plate (3) and the wedge-shaped plug-in plate (4) are sequentially arranged in the body glue tank (1) from left to right.

2. The gel making tank for protein vertical electrophoresis gel according to claim 1, characterized in that, The wedge-shaped plug-in plate (4) is composed of a large wedge-shaped plug-in plate (401), a middle wedge-shaped plug-in plate (402) and a small wedge-shaped plug-in plate (403), and the large wedge-shaped plug-in plate (401), the middle wedge-shaped plug-in plate (402) and the small wedge-shaped plug-in plate (403) are sequentially arranged on the surface of the inclined front plate (103).

3. The gel making tank for vertical protein electrophoresis according to claim 1, characterized in that, The upper surface of the bottom plate (101) is fixedly connected with two flow guide plates (7), the two flow guide plates (7) are fixedly connected with the inner sides of the vertical side plates (102), the left end of the flow guide plate (7) is fixedly connected with the right side of the vertical rear plate (104), and the right end of the flow guide plate (7) is fixedly connected with the surface of the inclined front plate (103).

4. The gel casting groove for protein vertical electrophoresis gel according to claim 3, characterized in that, A flow guide glue tank (8) is arranged between the two flow guide plates (7), the right end of the flow guide glue tank (8) is communicated with the bottom end of the lower glue pouring glue tank (5), and the size of the flow guide glue tank (8) is matched with that of the lower glue pouring glue tank (5).

5. The gel casting groove for protein vertical electrophoresis gel according to claim 1, characterized in that, The outer side of the vertical side plate (102) is fixedly connected with a strip-shaped baffle (9), and the position of the strip-shaped baffle (9) corresponds to the upper glue pouring glue tank (6).

6. The gel casting groove for protein vertical electrophoresis gel according to claim 2, characterized in that, The upper surfaces of the large wedge-shaped plug-in plate (401), the middle wedge-shaped plug-in plate (402) and the small wedge-shaped plug-in plate (403) are fixedly connected with limiting strip blocks (404), and the two ends of the limiting strip blocks (404) are arranged on the upper surface of the vertical side plate (102).

7. The gel casting groove for protein vertical electrophoresis gel according to claim 1, characterized in that, A plurality of protein glue glass plates (10) are arranged between the plurality of thin partition plates (3), and the plurality of protein glue glass plates (10) and the thin partition plates (3) are distributed in the area between the thick filling plate (2) and the wedge-shaped plug-in plate (4).

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