Non-contact type biological particle treatment equipment and biological particle treatment device

Abstract

The utility model provides non-contact biological particle treatment equipment and a biological particle treatment device. The biological particle processing device comprises a liquid droplet generating chamber, a working chamber communicated with the liquid droplet generating chamber, and a sorting chamber communicated with the working chamber. The droplet generating chamber is configured to receive a first liquid, a biological particle located within the first liquid, and a second liquid insoluble in the first liquid. The droplet generating chamber is used for enabling the flow of the second liquid to be staggered with the flow of the first liquid, so that the biological particles and the first liquid part around the biological particles jointly generate a biological particle droplet flowing to the working chamber after penetrating through the second liquid; the biological particles are cultured or tested through the first liquid. Therefore, the biological particle liquid drop can protect at least one biological particle located in the biological particle liquid drop, and culture work or detection can be completed while the biological particle liquid drop moves.

Description

TECHNICAL FIELD

[0001] The present application relates to a biological particle processing system, in particular to a non-contact biological particle processing device and a biological particle processing apparatus. BACKGROUND

[0002] The existing biological particle processing system can perform related operations on a biological particle in a liquid, but based on the biological particle flowing with the liquid, so the existing biological particle processing system is fixed by the fixed point method to perform related operations, but this is obviously not conducive to the cultivation of the biological particle. Moreover, whether the biological particle flows with the liquid or is fixed, the biological particle is easily affected or harmed by the pressure of the liquid.

[0003] Therefore, the applicant believes that the above defects can be improved, and finally proposes the present application by careful research and application of scientific principles. CONTENT OF THE UTILITY MODEL

[0004] The technical problem to be solved by the present application is to provide a non-contact biological particle processing device and a biological particle processing apparatus, which can effectively improve the defects that may be generated by the existing biological particle processing system.

[0005] The embodiment of the present application discloses a non-contact biological particle processing device, which comprises: a biological particle processing apparatus for receiving a first liquid and a second liquid immiscible with the first liquid; wherein the biological particle processing apparatus comprises: a droplet generating chamber for containing the first liquid and at least one biological particle in the first liquid; wherein the droplet generating chamber is used to generate a biological particle droplet with the at least one biological particle and the surrounding part of the first liquid; a working chamber connected to the droplet generating chamber; wherein the working chamber is used to contain the second liquid and the biological particle droplet, so that the biological particle droplet can flow in the second liquid of the working chamber, and the first liquid of the biological particle droplet is used to perform a cultivation operation or a detection operation on the at least one biological particle; a sorting chamber connected to the working chamber; wherein the sorting chamber is used to contain the first liquid, so that an immiscible interface is generated between the working chamber and the sorting chamber; a light driving device facing the biological particle processing apparatus; wherein the light driving device can be used to drive the biological particle processing apparatus to generate a dielectrophoresis pattern to move the biological particle droplet; wherein the light driving device can move the biological particle droplet from the working chamber to the sorting chamber through the dielectrophoresis pattern, so that the first liquid of the biological particle droplet dissolves into the first liquid of the sorting chamber, and the at least one biological particle is released into the first liquid of the sorting chamber.

[0006] Optionally, the droplet generation chamber is configured to receive the second liquid and to interleave a flow of the second liquid with the first liquid to collectively generate the biological particle droplet from the at least one biological particle and the portion of the first liquid surrounding the at least one biological particle after passing through the second liquid.

[0007] Optionally, the droplet generation chamber comprises a first flow path configured to receive the first liquid and the at least one biological particle, and a second flow path interleaved with the first flow path to generate a confluence region in communication with the working chamber, wherein the second flow path is configured to receive the second liquid and to interleave a flow of the second liquid with the first liquid to collectively generate the biological particle droplet from the at least one biological particle and the portion of the first liquid surrounding the at least one biological particle after passing through the confluence region.

[0008] Optionally, the working chamber is configured to generate a waste port, and the optical driving device is configured to selectively move the biological particle droplet from the working chamber to the sorting chamber or the waste port by the dielectrophoretic pattern.

[0009] Optionally, the first liquid of the biological particle droplet comprises at least one of a culture medium, a peptide, and a recombinant protein for performing a culture operation on the at least one biological particle, or the first liquid of the biological particle droplet comprises at least one of a detection reagent and a chemical reagent for performing a detection operation on the at least one biological particle.

[0010] Optionally, the biological particle processing device comprises an optical sensing module comprising a first substrate, a first electrode layer generated on the first substrate, and a photoelectric layer generated on the first substrate, and a cooperating module spaced apart from the optical sensing module, and at least one of the optical sensing module and the cooperating module is transparent, wherein the cooperating module comprises a second substrate and a second electrode layer generated on the second substrate, and the second electrode layer faces the optical sensing module, and the optical driving device is configured to emit light to irradiate the optical sensing module to generate the dielectrophoretic pattern on the optical sensing module.

[0011] The embodiments of the present application also disclose a non-contact biological particle processing device, which comprises a biological particle processing device for receiving a first liquid and a second liquid immiscible with the first liquid; wherein the biological particle processing device comprises a droplet generating chamber for containing the first liquid and at least one biological particle in the first liquid; wherein the droplet generating chamber is used to generate a biological particle droplet with the at least one biological particle and the part of the first liquid around the at least one biological particle; a working chamber communicated with the droplet generating chamber; wherein the working chamber is used to contain the second liquid and the biological particle droplet, so that the biological particle droplet can flow in the second liquid of the working chamber, and the first liquid of the biological particle droplet is used to perform a culture operation or a detection operation on the at least one biological particle; a sorting chamber communicated with the working chamber and generating a release structure adjacent to the edge of the working chamber; wherein the sorting chamber is used to contain the second liquid; and a light driving device facing the biological particle processing device; wherein the light driving device can be used to drive the biological particle processing device to generate a dielectrophoresis pattern to move the biological particle droplet; and wherein the light driving device can be used to move the biological particle droplet from the working chamber to the sorting chamber along the release structure through the dielectrophoresis pattern, so that the biological particle droplet is broken by the release structure, the first liquid of the biological particle droplet is dispersed, and the at least one biological particle is released into the second liquid of the sorting chamber.

[0012] Optionally, the droplet generating chamber is used to contain the second liquid, and the flow of the second liquid is interleaved with the first liquid, so that the at least one biological particle and the part of the first liquid around the at least one biological particle generate the biological particle droplet after passing through the second liquid.

[0013] Optionally, the droplet generating chamber comprises a first flow channel for inputting the first liquid and the at least one biological particle, and a second flow channel interleaved with the first flow channel to generate a confluence area communicated with the working chamber; wherein the second flow channel is used to input the second liquid, and the at least one biological particle and the part of the first liquid around the at least one biological particle generate the biological particle droplet after passing through the confluence area.

[0014] Optionally, the working chamber generates a waste port, and the light driving device can be used to selectively move the biological particle droplet from the working chamber to the sorting chamber or the waste port through the dielectrophoresis pattern.

[0015] Optionally, the first liquid of the biological particle droplet comprises at least one of a culture medium, a peptide and a recombinant protein, and is used to perform the culture operation on the at least one biological particle; or the first liquid of the biological particle droplet comprises at least one of a detection reagent and a chemical reagent, and is used to perform the detection operation on the at least one biological particle.

[0016] Optionally, the bioparticle processing device includes: a photosensitive module, comprising a first substrate, a first electrode layer formed on the first substrate, a photoelectric layer formed on the first substrate, and an insulating layer formed on the photoelectric layer; a mating module, spaced apart from the photosensitive module, wherein at least one of the photosensitive module and the mating module is transparent; wherein the mating module includes a second substrate and a second electrode layer formed on the second substrate; wherein the light driving device is used to emit light to irradiate the photosensitive module, so that the photosensitive module generates a dielectric electrophoretic pattern.

[0017] Optionally, the density of the first liquid is greater than the density of the second liquid, and the release structure is formed in the insulating layer.

[0018] Optionally, the density of the first liquid is less than the density of the second liquid, and the release structure is generated in the mating module.

[0019] This application also discloses a bioparticle processing device for receiving a first liquid and a second liquid immiscible with the first liquid. The bioparticle processing device includes: a droplet generation chamber for containing the first liquid, at least one bioparticle located within the first liquid, and the second liquid; wherein the droplet generation chamber is used to allow the flow of the second liquid to interweave with the first liquid, so that at least one bioparticle and the portion of the first liquid surrounding it, after passing through the second liquid, jointly generate a bioparticle droplet; a working chamber connected to the droplet generation chamber; wherein the working chamber is used to contain the second liquid, so that the bioparticle droplet can flow within the second liquid in the working chamber, and the first liquid of the bioparticle droplet is used to perform a culture operation or a detection operation on at least one bioparticle; and a sorting chamber connected to the working chamber.

[0020] Optionally, the sorting chamber is used to contain a first liquid to create an immiscible interface between the working chamber and the sorting chamber; wherein, when the bioparticle droplets move from the working chamber to the sorting chamber, the first liquid of the bioparticle droplets dissolves into the first liquid of the sorting chamber to release at least one bioparticle into the first liquid of the sorting chamber.

[0021] Optionally, the sorting chamber is used to contain a second liquid, and a release structure is formed at the edge of the sorting chamber adjacent to the working chamber; wherein, when the bioparticle droplets move from the working chamber to the sorting chamber via the release structure, the bioparticle droplets are disrupted by the release structure, so that the first liquid of the bioparticle droplets is dispersed, thereby releasing at least one bioparticle into the second liquid of the sorting chamber.

[0022] Optionally, the biological particle processing device comprises: a light sensing module, comprising a first substrate, a first electrode layer formed on the first substrate, a photoelectric layer formed on the first substrate, and an insulating layer formed on the photoelectric layer; a matching module, spaced from the light sensing module, and at least one of the light sensing module and the matching module is transparent; wherein the matching module comprises a second substrate and a second electrode layer formed on the second substrate; wherein the density of the first liquid is greater than the density of the second liquid, and the release structure is formed on the insulating layer.

[0023] Optionally, the biological particle processing device comprises: a light sensing module, comprising a first substrate, a first electrode layer formed on the first substrate, a photoelectric layer formed on the first substrate, and an insulating layer formed on the photoelectric layer; a matching module, spaced from the light sensing module, and at least one of the light sensing module and the matching module is transparent; wherein the matching module comprises a second substrate and a second electrode layer formed on the second substrate; wherein the density of the first liquid is less than the density of the second liquid, and the release structure is formed on the matching module.

[0024] Optionally, the droplet generating chamber comprises: a first flow channel for inputting the first liquid and at least one biological particle; a second flow channel, interleaved with the first flow channel, to form a confluence area connected to the working chamber; wherein the second flow channel is used to input the second liquid, and the at least one biological particle and the part of the first liquid around it generate a biological particle droplet after passing through the confluence area.

[0025] In summary, the non-contact biological particle processing equipment and biological particle processing device disclosed in the embodiments of the present application can generate biological particle droplets suspended in the second liquid, so that at least one biological particle is protected by being covered in the first liquid, and the biological particle droplets can move quickly in the second liquid without damaging the at least one biological particle located therein, and the biological particle droplets can complete the culture work or the detection work of the at least one biological particle located therein while moving.

[0026] The details of other functions and embodiments of the present application are described below with reference to the drawings. BRIEF DESCRIPTION OF DRAWINGS

[0027] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiment or prior art description will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments described in the present application, and those skilled in the art can also obtain other drawings according to these drawings without creative labor.

[0028] Figure 1 Fig. 1 is a perspective view of a non-contact biological particle processing apparatus according to an embodiment of the present application;

[0029] Figure 2 Fig. 2 is a longitudinal sectional view of the non-contact biological particle processing apparatus according to the embodiment of the present application; Figure 1

[0030] Figure 3 Fig. 3 is a transverse sectional view of the non-contact biological particle processing apparatus according to the embodiment of the present application;

[0031] Figure 4 Fig. 4 is a transverse sectional view of the non-contact biological particle processing apparatus according to the embodiment of the present application, in which a plurality of biological particle droplets are generated; Figure 3

[0032] Figure 5 Fig. 5 is a longitudinal sectional view of the non-contact biological particle processing apparatus according to the embodiment of the present application;

[0033] Figure 6 Fig. 6 is a subsequent operation schematic view of the non-contact biological particle processing apparatus according to the embodiment of the present application; Figure 3

[0034] Fig. 7 is a subsequent operation schematic view of the non-contact biological particle processing apparatus according to the embodiment of the present application; Figure 7 Figure 6 Fig. 8 is a transverse sectional view of another aspect of the non-contact biological particle processing apparatus according to the embodiment of the present application;

[0035] Figure 8 Fig. 9 is a transverse sectional view of a non-contact biological particle processing apparatus according to another embodiment of the present application;

[0036] Figure 9 Fig. 10 is a longitudinal sectional view of the non-contact biological particle processing apparatus according to the another embodiment of the present application;

[0037] Figure 10 Figure 9 Fig. 11 is a subsequent operation schematic view of the non-contact biological particle processing apparatus according to the another embodiment of the present application;

[0038] Figure 11 Fig. 12 is a longitudinal sectional view of the non-contact biological particle processing apparatus according to the another embodiment of the present application; Figure 9

[0039] Fig. 13 is a subsequent operation schematic view of the non-contact biological particle processing apparatus according to the another embodiment of the present application; Figure 12 Figure 11 Fig. 14 is a longitudinal sectional view of the non-contact biological particle processing apparatus according to the another embodiment of the present application;

[0040] Figure 13 Figure 11 Fig. 15 is a subsequent operation schematic view of the non-contact biological particle processing apparatus according to the another embodiment of the present application;

[0041] Figure 14 Fig. 16 is a longitudinal sectional view of the non-contact biological particle processing apparatus according to the another embodiment of the present application; Figure 13

[0042] ​​​​​​​Figure 15 This is a cross-sectional schematic diagram of another aspect of the non-contact biological particle treatment device according to Embodiment 2 of this application;

[0043] Figure 16 for Figure 15 A longitudinal cross-sectional schematic diagram of a non-contact biological microparticle treatment device;

[0044] Figure 17 for Figure 15 A schematic diagram of the subsequent operation;

[0045] Figure 18 for Figure 17 A longitudinal cross-sectional view of a non-contact biological microparticle treatment device. Detailed Implementation

[0046] The following specific embodiments illustrate the implementation of the "non-contact biological particle treatment device and biological particle treatment apparatus" disclosed in this application. Those skilled in the art can understand the advantages and effects of this application from the content disclosed in this specification. This application can be implemented or applied through other different specific embodiments, and various details in this specification can also be modified and changed based on different viewpoints and applications without departing from the concept of this application. Furthermore, the accompanying drawings of this application are for simple illustrative purposes only and are not depictions of actual dimensions, as stated in advance. The following embodiments will further describe the relevant technical content of this application in detail, but the disclosed content is not intended to limit the scope of protection of this application.

[0047] It should be understood that while terms such as "first," "second," and "third" may be used in this document to describe various components or features, these components or features should not be limited by these terms. These terms are primarily used to distinguish one component from another, or one feature from another. Furthermore, the term "or" as used in this document should, as appropriate, include any combination of one or more related listed items.

[0048] [Example 1]

[0049] Please see Figures 1 to 8 As shown, this is an embodiment of this application. Figures 1 to 3 As shown, this embodiment discloses a non-contact biological particle processing device 100, which is used to perform a culture or detection operation on at least one biological particle B. The biological particle B can be a specific type of cell or cell cluster, such as circulating tumor cells (CTCs), fetal nucleated red blood cells (FNRBCs), viruses, microorganisms, or bacteria, but this application is not limited to the above.

[0050] The non-contact bio-particle processing apparatus 100 includes a bio-particle processing device 1, an alternating current device 2 electrically coupled to the bio-particle processing device 1, and a light driving device 3 facing the bio-particle processing device 1, but the present application is not limited thereto. For example, in other embodiments not shown in the present application, the bio-particle processing device 1 can be independently applied (e.g., sold) or used with other devices according to actual needs.

[0051] The bio-particle processing device 1 in the present embodiment adopts a rectangular shape of chip-scale size, and is used to receive (or includes) a first liquid L1, at least one bio-particle B located in the first liquid L1, and a second liquid L2 that is not miscible with the first liquid L1. For example, the first liquid L1 can include oil and a surfactant, and the second liquid L2 is water; or the first liquid L1 can include water and a surfactant, and the second liquid L2 is oil, but the present application is not limited thereto.

[0052] In addition, the light driving device 3 can be used to drive the bio-particle processing device 1 to generate a dielectrophoresis (DEP) pattern F (e.g., Figure 6 ), and the corresponding structure of the bio-particle processing device 1 for achieving the DEP pattern F is generally described as follows, but the present application is not limited thereto.

[0053] In the present embodiment, the bio-particle processing device 1 includes a light sensing module 11, a matching module 12 spaced from the light sensing module 11, and a bonding layer 13 bonding the periphery of the light sensing module 11 and the periphery of the matching module 12. Among them, at least one of the light sensing module 11 and the matching module 12 is transparent, and the light sensing module 11 and the matching module 12 in the present embodiment are two plate-shaped structures arranged parallel to each other and the distance between them is greater than the size of any bio-particle B, but the present application is not limited thereto.

[0054] In more detail, the light sensing module 11 has a first substrate 111, a first electrode layer 112 formed on the first substrate 111, and a photoelectric layer 113 formed on the first substrate 111. In the present embodiment, the first electrode layer 112 is formed on the bottom side of the first substrate 111, and the photoelectric layer 113 is formed on the top side of the first substrate 111. The photoelectric layer 113 can be formed with a plurality of transistors arranged in a matrix, and the photoelectric layer 113 can adopt an NPN transistor architecture, a PNP transistor architecture, an NP diode architecture, or a PN diode architecture according to actual needs, but the present application is not limited thereto.

[0055] The matching module 12 includes a second substrate 121 and a second electrode layer 122 formed on the second substrate 121, and the second electrode layer 122 faces the light sensing module 11 (e.g., the photoelectric layer 113). The alternating current device 2 is electrically coupled to the first electrode layer 112 of the light sensing module 11 and the second electrode layer 122 of the matching module 12.

[0056] Thus, as shown in Figure 2 and Figures 5 to 7 The light driving device 3 can be used to emit light to irradiate the light sensing module 11, so that the light sensing module 11 generates the dielectrophoresis pattern F. In the present embodiment, the light driving device 3 can include a camera 31 and a light source 32 matched with the camera 31. The light driving device 3 can emit light through the light source 32 to irradiate the light sensing module 11, so that the light sensing module 11 (or the photoelectric layer 113) generates the dielectrophoresis pattern F.

[0057] From another perspective, the internal structure of the biological particle processing device 1 includes a droplet generation chamber 14, a working chamber 15 communicated with the droplet generation chamber 14, and a sorting chamber 16 communicated with the working chamber 15. In the present embodiment, the droplet generation chamber 14, the working chamber 15, and the sorting chamber 16 are arranged between the light sensing module 11 and the matching module 12, and the droplet generation chamber 14 and the sorting chamber 16 are respectively communicated with opposite sides of the working chamber 15 in the present embodiment, but the present application is not limited thereto.

[0058] The droplet generation chamber 14 is used to accommodate the first liquid L1 and at least one biological particle B located in the first liquid L1. The droplet generation chamber 14 is used to generate a biological particle droplet P with at least one biological particle B and the first liquid L1 around it.

[0059] It is to be noted that, for the purpose of understanding the present embodiment, the following description will be made with the assumption that one of the biological particle droplets P is generated in the biological particle processing device 1. However, the present application is not limited thereto. For example, as shown in Figure 4 as shown in FIG. 1, a plurality of the biological particle droplets P can be generated in the biological particle processing device 1, and the number of the biological particles B in any one of the biological particle droplets P can be greater than one according to actual needs.

[0060] Further, the droplet generating chamber 14 can be designed according to actual needs on the premise that the biological particle droplet P can be generated. For example, in other embodiments not shown in the present application, the droplet generating chamber 14 can disperse the first liquid LI into a plurality of droplets by physical means (such as stirring or shaking), and the droplet coated with at least one of the biological particles B is defined as the biological particle droplet P.

[0061] In addition, the droplet generating chamber 14 generates the biological particle droplet P by fluid means in the present embodiment, so as to reduce the possible damage to the biological particles B. The droplet generating chamber 14 is used to further contain the second liquid L2, and the flow of the second liquid L2 is interleaved with the first liquid LI, so that at least one of the biological particles B and the portion of the first liquid LI around it generate the biological particle droplet P together after passing through the second liquid L2.

[0062] In more detail, the droplet generating chamber 14 includes a first flow channel 141 and a second flow channel 142 (vertically) interleaved with the first flow channel 141, and the first flow channel 141 and the second flow channel 142 are interleaved with each other to generate a confluence region 143 communicating with the working chamber 15. The first flow channel 141 is used to input the first liquid LI and at least one of the biological particles B, and the second flow channel 142 is used to input the second liquid L2, so that at least one of the biological particles B and the portion of the first liquid LI around it generate the biological particle droplet P together after passing through the confluence region 143.

[0063] The working chamber 15 is used to contain the second liquid L2 and the biological particle droplet P, so that the biological particle droplet P can flow in the second liquid L2 in the working chamber 15, and the biological particle droplet P can be moved (such as pushed) in the working chamber 15 by the dielectrophoresis pattern F. Further, the biological particle droplet P in the working chamber 15 can perform a culture operation or a detection operation on at least one of the biological particles B in it by the first liquid LI.

[0064] In the present embodiment, the first liquid L1 of the biological particle droplet P includes at least one of a medium, a peptide, and a recombinant protein for performing the culture operation on at least one of the biological particles B; or the first liquid L1 of the biological particle droplet P includes at least one of a detection reagent and a chemical for performing the detection operation on at least one of the biological particles B.

[0065] As described above, the biological particle processing device 1 in the present embodiment can achieve the effect of protecting at least one of the biological particles B by encapsulating the biological particle droplet P in the second liquid L2, so that the biological particle droplet P can move rapidly in the second liquid L2 without damaging at least one of the biological particles B, and can also complete the culture operation or the detection operation on at least one of the biological particles B while moving.

[0066] It should be noted that the operation chamber 15 is provided with a waste port 151, and the optical driving device 3 can use the dielectrophoresis pattern F to selectively move the biological particle droplet P from the operation chamber 15 to the sorting chamber 16 or the waste port 151. That is, after the biological particle droplet P performs the culture operation or the detection operation, if the result is a failure, the biological particle droplet P will be moved to the waste port 151 by the dielectrophoresis pattern F, and then removed from the biological particle processing device 1; if the result is a success, the biological particle droplet P will be moved into the sorting chamber 16 by the dielectrophoresis pattern F.

[0067] Further, the sorting chamber 16 is used to contain the first liquid L1, so that an immiscible interface L3 is generated between the operation chamber 15 and the sorting chamber 16. Thus, the optical driving device 3 can use the dielectrophoresis pattern F to move the biological particle droplet P from the operation chamber 15 to the sorting chamber 16, so that the first liquid L1 of the biological particle droplet P dissolves into the first liquid L1 of the sorting chamber 16, and at least one of the biological particles B is released into the first liquid L1 of the sorting chamber 16.

[0068] In addition, the sorting chamber 16 can further have a collection port 161, and at least one of the biological particles B in the sorting chamber 16 can be removed from the biological particle processing device 1 through the collection port 161. The movement of at least one of the biological particles B in the sorting chamber 16 can be achieved by the dielectrophoresis pattern F (as shown in Figure 6 and Figure 7 ) or hydraulic control (as shown in Figure 8 further provided with a hydraulic control port 163).

[0069] [Embodiment 2]

[0070] Please refer to Figures 9 to 18 , which is the embodiment 2 of the present application. Since the embodiment is similar to the above-mentioned embodiment 1, the same parts of the two embodiments will not be described again, and the differences between the embodiment and the above-mentioned embodiment 1 mainly lie in the sorting chamber 16.

[0071] In the embodiment, the sorting chamber 16 is used to contain the second liquid L2, the light sensing module 11 includes an insulating layer 114 generated on the photoelectric layer 113, and the sorting chamber 16 has a release structure 162 generated on the edge adjacent to the working chamber 15, which is used to break the surface tension of the biological particle droplet P. In the embodiment, the release structure 162 includes a plurality of protrusions 1621 arranged along the edge of the working chamber 15, and the distance between any two adjacent protrusions 1621 can be selected to be less than the outer diameter of the biological particle droplet P, but the present application is not limited thereto.

[0072] It should be noted that, as shown in Figures 9 to 14 , when the density of the first liquid L1 used by the biological particle processing device 1 is greater than the density of the second liquid L2, the biological particle droplet P is easy to sink in the second liquid L2, so the release structure 162 is generated on the insulating layer 114. Furthermore, as shown in Figures 15 to 18 , when the density of the first liquid L1 used by the biological particle processing device 1 is less than the density of the second liquid L2, the biological particle droplet P is easy to float in the second liquid L2, so the release structure 162 is generated on the matching module 12.

[0073] As described above, the optical driving device 3 can use the dielectrophoresis pattern F to move the biological particle droplet P from the operation chamber 15 to the release structure 162, and then to the sorting chamber 16, so that the biological particle droplet P is broken by the release structure 162, and the first liquid L1 of the biological particle droplet P is dispersed, and at least one biological particle B is released into the second liquid L2 in the sorting chamber 16.

[0074] [Technical effects of the embodiments of the present application]

[0075] As described above, the non-contact biological particle processing device and the biological particle processing apparatus disclosed in the embodiments of the present application can generate the biological particle droplet suspended in the second liquid, so that at least one biological particle is protected by being covered in the first liquid, and the biological particle droplet can move quickly in the second liquid without damaging at least one biological particle located therein, and the biological particle droplet can complete the culture operation or the detection operation of at least one biological particle located therein while moving.

[0076] The above-described embodiments and / or implementations are used to illustrate the preferred embodiments and / or implementations of the present application, and do not limit the embodiments of the present application in any form. Any person skilled in the art can make some changes or modifications to other equivalent embodiments without departing from the scope of the technical means disclosed in the present application, but should be considered as the same technology or embodiment as the present application.

Claims

1. A non-contact biological particle treatment device, characterized in that, The non-contact biological particle treatment device includes: A bioparticle processing device for receiving a first liquid and a second liquid immiscible with the first liquid; wherein the bioparticle processing device comprises: A droplet generation chamber for containing a first liquid and at least one biological microparticle located within the first liquid; wherein the droplet generation chamber is used for at least one biological microparticle and the surrounding portion of the first liquid to generate a biological microparticle droplet; A working chamber, connected to the droplet generation chamber; wherein the working chamber is used to contain the second liquid and the bioparticle droplets, so that the bioparticle droplets can flow within the second liquid in the working chamber, and the first liquid of the bioparticle droplets is used to perform a culture operation or a detection operation on at least one of the bioparticles; and A sorting chamber connected to the working chamber; wherein the sorting chamber is used to contain the first liquid, thereby creating an immiscible interface between the working chamber and the sorting chamber; and A light-driven device is provided facing the bioparticle processing device; wherein the light-driven device is used to drive the bioparticle processing device to generate a dielectric electrophoretic pattern to move the bioparticle droplets. The light-driven device can move the biological microparticle droplets from the working chamber to the sorting chamber through the dielectric electrophoresis pattern, so that the first liquid of the biological microparticle droplets dissolves into the first liquid of the sorting chamber, thereby releasing at least one biological microparticle into the first liquid of the sorting chamber.

2. The non-contact biological particle treatment device according to claim 1, characterized in that, The droplet generation chamber is used to contain the second liquid and to allow the flow of the second liquid to interweave with the first liquid, so that at least one of the biological microparticles and the portion of the first liquid surrounding them, after passing through the second liquid, jointly generate the biological microparticle droplets.

3. The non-contact biological particle treatment device according to claim 2, characterized in that, The droplet generation chamber includes: A first flow channel for introducing the first liquid and at least one of the said biological microparticles; and A second flow channel, intersecting the first flow channel, to generate a confluence region communicating with the working chamber; wherein the second flow channel is used to input the second liquid and to cause at least one of the bioparticles and the portion of the first liquid surrounding them to co-generate the bioparticle droplets after passing through the confluence region.

4. The non-contact biological particle treatment device according to claim 1, characterized in that, The working chamber has a waste outlet, and the photo-driven device can selectively move the biological microparticle droplets from the working chamber to the sorting chamber or the waste outlet using the dielectric electrophoresis pattern.

5. The non-contact biological particle treatment device according to claim 1, characterized in that, The bioparticle processing device includes: A photosensitive module includes a first substrate, a first electrode layer formed on the first substrate, and a photoelectric layer formed on the first substrate; and A mating module is spaced apart from the photosensing module, and at least one of the photosensing module and the mating module is transparent; wherein the mating module includes a second substrate and a second electrode layer formed on the second substrate, and the second electrode layer faces the photosensing module; The light-driving device can be used to emit light to illuminate the light-sensing module, so that the light-sensing module generates the dielectric electrophoretic pattern.

6. A non-contact biological particle treatment device, characterized in that, The non-contact biological particle treatment device includes: A bioparticle processing device for receiving a first liquid and a second liquid immiscible with the first liquid; wherein the bioparticle processing device comprises: A droplet generation chamber for containing a first liquid and at least one biological microparticle located within the first liquid; wherein the droplet generation chamber is used for at least one biological microparticle and the surrounding portion of the first liquid to generate a biological microparticle droplet; A working chamber, connected to the droplet generation chamber; wherein the working chamber is used to contain the second liquid and the bioparticle droplets, so that the bioparticle droplets can flow within the second liquid in the working chamber, and the first liquid of the bioparticle droplets is used to perform a culture operation or a detection operation on at least one of the bioparticles; and A sorting chamber, communicating with the working chamber and having a release structure formed at an edge adjacent to the working chamber; wherein the sorting chamber is used to contain the second liquid; and A light-driven device is provided facing the bioparticle processing device; wherein the light-driven device is used to drive the bioparticle processing device to generate a dielectric electrophoretic pattern to move the bioparticle droplets. The light-driven device can use the dielectric electrophoresis pattern to move the biological microparticle droplets from the working chamber to the sorting chamber along the release structure, so that the biological microparticle droplets are destroyed by the release structure, thereby dispersing the first liquid of the biological microparticle droplets and releasing at least one biological microparticle into the second liquid of the sorting chamber.

7. The non-contact biological particle treatment device according to claim 6, characterized in that, The droplet generation chamber is used to contain the second liquid and to allow the flow of the second liquid to interweave with the first liquid, so that at least one of the biological microparticles and the portion of the first liquid surrounding them, after passing through the second liquid, jointly generate the biological microparticle droplets.

8. The non-contact biological particle treatment device according to claim 7, characterized in that, The droplet generation chamber includes: A first flow channel for introducing the first liquid and at least one of the said biological microparticles; and A second flow channel, intersecting the first flow channel, to generate a confluence region communicating with the working chamber; wherein the second flow channel is used to input the second liquid and to cause at least one of the bioparticles and the portion of the first liquid surrounding them to co-generate the bioparticle droplets after passing through the confluence region.

9. The non-contact biological particle treatment device according to claim 6, characterized in that, The working chamber has a waste outlet, and the photo-driven device can selectively move the biological microparticle droplets from the working chamber to the sorting chamber or the waste outlet using the dielectric electrophoresis pattern.

10. The non-contact biological particle treatment device according to claim 6, characterized in that, The bioparticle processing device includes: A photosensitive module includes a first substrate, a first electrode layer formed on the first substrate, a photoelectric layer formed on the first substrate, and an insulating layer formed on the photoelectric layer; and A mating module is spaced apart from the photosensing module, and at least one of the photosensing module and the mating module is transparent; wherein the mating module includes a second substrate and a second electrode layer formed on the second substrate; The light-driving device can be used to emit light to illuminate the light-sensing module, so that the light-sensing module generates the dielectric electrophoretic pattern.

11. The non-contact biological particle treatment device according to claim 10, characterized in that, The density of the first liquid is greater than the density of the second liquid, and the release structure is formed in the insulating layer.

12. The non-contact biological particle treatment device according to claim 10, characterized in that, The density of the first liquid is less than the density of the second liquid, and the release structure is generated in the cooperating module.

13. A biological microparticle treatment device, characterized in that, The bioparticle processing device is used to receive a first liquid and a second liquid immiscible with the first liquid, and the bioparticle processing device includes: A droplet generating chamber for containing a first liquid, at least one biological microparticle located within the first liquid, and a second liquid; wherein the droplet generating chamber is configured to allow the flow of the second liquid to interweave with the first liquid, such that at least one biological microparticle and the portion of the first liquid surrounding it, after passing through the second liquid, jointly generate a biological microparticle droplet; A working chamber connected to the droplet generation chamber; wherein the working chamber is used to contain the second liquid, allowing the bioparticle droplets to flow within the second liquid in the working chamber, and to perform a culture or detection operation on at least one of the bioparticles using the first liquid of the bioparticle droplets; and A sorting chamber is connected to the working chamber.

14. The biological microparticle treatment device according to claim 13, characterized in that, The sorting chamber is used to contain the first liquid so that an immiscible interface is formed between the working chamber and the sorting chamber; wherein, when the bioparticle droplet moves from the working chamber to the sorting chamber, the first liquid of the bioparticle droplet dissolves into the first liquid of the sorting chamber, so as to release at least one bioparticle into the first liquid of the sorting chamber.

15. The bioparticle treatment device according to claim 13, characterized in that, The sorting chamber is used to contain the second liquid, and a release structure is formed at the edge of the sorting chamber adjacent to the working chamber; wherein, when the bioparticle droplet moves from the working chamber to the sorting chamber via the release structure, the bioparticle droplet is disrupted by the release structure, so that the first liquid of the bioparticle droplet is dispersed, thereby releasing at least one bioparticle into the second liquid in the sorting chamber.

16. The biological microparticle treatment device according to claim 15, characterized in that, The bioparticle processing device includes: A photosensitive module includes a first substrate, a first electrode layer formed on the first substrate, a photoelectric layer formed on the first substrate, and an insulating layer formed on the photoelectric layer; and A mating module is spaced apart from the photosensing module, and at least one of the photosensing module and the mating module is transparent; wherein the mating module includes a second substrate and a second electrode layer formed on the second substrate; The density of the first liquid is greater than that of the second liquid, and the release structure is formed in the insulating layer.

17. The bioparticle treatment device according to claim 15, characterized in that, The bioparticle processing device includes: A photosensitive module includes a first substrate, a first electrode layer formed on the first substrate, a photoelectric layer formed on the first substrate, and an insulating layer formed on the photoelectric layer; and A mating module is spaced apart from the photosensing module, and at least one of the photosensing module and the mating module is transparent; wherein the mating module includes a second substrate and a second electrode layer formed on the second substrate; The density of the first liquid is less than that of the second liquid, and the release structure is generated in the cooperating module.

18. The bioparticle treatment device according to claim 13, characterized in that, The droplet generation chamber includes: A first flow channel for introducing the first liquid and at least one of the said biological microparticles; and A second flow channel, intersecting the first flow channel, to generate a confluence region communicating with the working chamber; wherein the second flow channel is used to input the second liquid and to cause at least one of the bioparticles and the portion of the first liquid surrounding them to co-generate the bioparticle droplets after passing through the confluence region.

Images