Preparation device for enzymolysis extraction of small molecule peptide

Abstract

The utility model relates to the technical field of peptide enzymolysis, in particular to a preparation device for enzymolysis extraction of small molecule peptides, which comprises an enzymolysis tank, a heat preservation sleeve is nested and connected on the outer surface of the enzymolysis tank, an auger shaft is rotatably connected in the enzymolysis tank, and a rotating shaft is fixedly connected at the upper end of the auger shaft in the enzymolysis tank. According to the utility model, the angle of the hinge rod is changed, and the fixed seat further drives the scraper to be close to the inner wall of the enzymolysis tank, so that the inner wall of the enzymolysis tank can be scraped through the scraper at the later stage of enzymolysis; the inner wall of the enzymolysis tank is prevented from being scraped all the time, the abrasion degree of the scraping plate is reduced, the positions of the first stirring blade and the second stirring blade are adjustable, raw materials and enzymes at different positions can be stirred conveniently, the stirring efficiency of the device is improved, and meanwhile after the outer surface of the scraping plate is abraded, the unabraded scraping plate can make contact with the inner wall of the enzymolysis tank.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of peptidase, and specifically relates to a preparation device for small molecule peptidase extraction. BACKGROUND

[0002] The defatted and decolored protein raw materials such as soybean, whey, fish and meat are put into the enzyme hydrolysis tank, then appropriate enzymes such as trypsin, papain and alkaline protease are selected according to the characteristics of the target small molecule peptides to perform enzyme hydrolysis reaction, then the liquid is introduced into the centrifugal separation equipment outside, and the undecomposed protein, enzymes and other impurities are removed through filtration, centrifugation, ultrafiltration and chromatography, which is mature prior art and will not be described in detail, so that the target small molecule peptides can be collected, but the device in the prior art has certain disadvantages in use, such as the preparation device for small molecule peptidase extraction disclosed in the publication No. CN216513894U, which relates to the technical field of active peptide extraction device.

[0003] The above device realizes complete discharge through the elastic force of the spring to make the scraper contact with the inner wall of the enzyme hydrolysis tank, first, the elastic force of the spring cooperates with the centrifugal force received by the scraper during stirring to make the scraper exert a pressure on the inner wall of the enzyme hydrolysis tank, the pressure increases the friction between the scraper and the enzyme hydrolysis tank, accelerates the wear of the scraper, and the bottom surface of the enzyme hydrolysis tank cannot be scraped, so that the bottom surface remains with materials, in addition, the position of the stirring blade cannot be adjusted, so that the stirring position is unchanged, and the materials cannot be fully mixed. TECHNICAL FIELD

[0004] The utility model aims at providing a preparation device for small molecule peptidase extraction to solve the problems in the background art.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical scheme:

[0006] The application discloses a preparation device for small-molecule peptide enzymolysis extraction, which comprises an enzymolysis tank, the outer surface of the enzymolysis tank is connected with a heat preservation sleeve in a nested mode, a screw shaft is rotatably connected in the inside of the enzymolysis tank, a rotating shaft is fixedly connected to the upper end of the screw shaft, a connecting sleeve is nestedly connected to the upper end of the rotating shaft, a first hinged seat is fixedly connected to the surface of the rotating shaft, a second hinged seat is movably connected to the surface of the rotating shaft, a hinged rod is hinged to the surfaces of the first hinged seat and the second hinged seat, a fixing seat is hinged to the other end of the hinged rod, an upper cover is fixedly connected to the upper surface of the enzymolysis tank, a hydraulic cylinder is fixedly connected to the upper surface of the upper cover, an installation plate is fixedly connected to the output end of the hydraulic cylinder, a speed reducer is fixedly connected to the upper surface of the installation plate, and a feeding tank is fixedly connected to the upper surface of the upper cover.

[0007] Further, the front surface of the heat preservation sleeve is fixedly connected with a controller, the front surface of the controller is rotatably connected with a temperature adjusting knob, the outer surface of the heat preservation sleeve is wound with a resistance wire, and the resistance wire is electrically connected with the controller.

[0008] Further, the inner surface of the fixing seat is fixedly connected with an L-shaped scraper, the outer surface of the hinged rod is fixedly connected with a first stirring blade, and the outer surface of the hinged rod is fixedly connected with a second stirring blade.

[0009] Further, the upper surface of the upper cover is fixedly connected with a spline seat near the central position, the inside of the spline seat is rotatably connected with a spline shaft, the connecting sleeve penetrates through the spline shaft, and the connecting sleeve and the spline shaft are slidably connected.

[0010] Further, the upper surface of the speed reducer is fixedly connected with a stepping motor, the output end of the stepping motor is fixedly connected with the input end of the speed reducer, the output end of the speed reducer is fixedly connected with the connecting sleeve, the upper surface of the upper cover is fixedly connected with a limiting rod, and the installation plate and the limiting rod are slidably connected.

[0011] Further, the connecting position of the upper cover and the feeding tank is nestedly connected with a feeding valve, and the inside of the upper cover is fixedly connected with a crushing tooth.

[0012] Further, the upper surface of the upper cover is detachably connected with a cover body, the upper surface of the cover body is fixedly connected with a crushing motor, and the output end of the crushing motor is fixedly connected with a crushing shaft.

[0013] Compared with the prior art, the application has the following beneficial effects:

[0014] The connecting sleeve can move up and down inside the spline shaft, the hydraulic cylinder is started, the output end of the hydraulic cylinder drives the mounting plate to rise or fall, the limiting rod limits the rising and falling of the mounting plate, the connecting sleeve drives the second hinge seat to rise and fall, the distance between the second hinge seat and the first hinge seat changes, the angle of the hinge rod changes, the fixed seat drives the scraper to be close to the inner wall of the enzymolysis tank, the inner wall of the enzymolysis tank can be scraped by the scraper in the later stage of enzymolysis, the inner wall of the enzymolysis tank is avoided from being scraped all the time, and the abrasion degree of the scraper is reduced;

[0015] The position of the first stirring blade and the second stirring blade can be adjusted, so that the raw materials and enzymes at different positions can be stirred, the stirring efficiency of the device is improved, and the un-abraded scraper can be in contact with the inner wall of the enzymolysis tank after the outer surface of the scraper is abraded. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is a whole schematic view of the utility model;

[0017] Figure 2 It is a cross-sectional structure schematic view of the enzymolysis tank of the utility model;

[0018] Figure 3 It is a hinge rod connecting structure schematic view of the utility model;

[0019] Figure 4 It is a cover body split structure schematic view of the utility model.

[0020] In the drawing: 1, heat preservation sleeve; 101, controller; 102, temperature adjusting knob; 103, resistance wire; 2, enzymolysis tank; 201, auger shaft; 203, rotating shaft; 3, first hinge seat; 301, second hinge seat; 302, fixed seat; 303, scraper; 304, hinge rod; 305, first stirring blade; 306, second stirring blade; 4, spline seat; 401, spline shaft; 5, mounting plate; 501, reduction box; 502, stepping motor; 503, hydraulic cylinder; 504, limiting rod; 505, connecting sleeve; 6, upper cover; 601, feeding tank; 602, feeding valve; 603, crushing tooth; 7, cover body; 701, crushing motor; 702, crushing shaft. DETAILED DESCRIPTION

[0021] The technical scheme in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Apparently, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0022] Please refer to Figures 1-4The utility model discloses an embodiment of a kind of small molecule peptide enzymatic extraction preparation devices, including enzymolysis jar 2, the outer surface of enzymolysis jar 2 is nested connection with heat preservation sleeve 1, the inside rotation of enzymolysis jar 2 is connected with auger shaft 201, the inside of enzymolysis jar 2 is fixedly connected with pivot shaft 203 at the upper end of auger shaft 201, pivot shaft 203 upper end is nested connection with connecting sleeve 505, pivot shaft 203 surface is fixedly connected with No. 1 hinged seat 3, pivot shaft 203 surface is movably connected with No. 2 hinged seat 301, No. 1 hinged seat 3 and No. 2 hinged seat 301 surface are hinged with hinged rod 304, the other end of hinged rod 304 is hinged with fixed seat 302, the upper surface of enzymolysis jar 2 is fixedly connected with upper cover 6, the upper surface of upper cover 6 is fixedly connected with hydraulic cylinder 503, the output of hydraulic cylinder 503 is fixedly connected with mounting plate 5, the upper surface of mounting plate 5 is fixedly connected with reduction box 501, the upper surface of upper cover 6 is fixedly connected with feeding tank 601.

[0023] Specifically, the defatted and decolorized protein raw materials such as soybean, whey, fish, meat and the like are put into the door body on the cover 7, crushed by the feeding tank 601 and then enter the enzymolysis jar 2, and then suitable enzymes are selected according to the characteristics of the target small molecule peptides, such as trypsin, papain, alkaline protease and the like, to perform enzymolysis reaction, and the controller 101 is started to heat the enzymolysis jar 2 to terminate the reaction, and then the valve at the lower end of the enzymolysis jar 2 is opened to make the liquid enter the centrifugal separation equipment outside, and the undecomposed protein, enzymes and other impurities are removed by filtering, centrifuging, ultrafiltration, chromatography and the like, which is mature prior art and will not be described herein, and the target small molecule peptides can be collected. In this process, the feeding tank 601 is used to make the raw materials more crushed, facilitating the later enzyme reaction, and the cooperation of the hinged rod 304, the No. 1 hinged seat 3 and the No. 2 hinged seat 301 makes the distance between the fixed seat 302 and the inner wall of the enzymolysis jar 2 adjustable, facilitating the cleaning of the inner wall of the enzymolysis jar 2 and the stirring of different positions in the enzymolysis jar 2.

[0024] Embodiment one

[0025] As shown in the drawings, Figures 1-2 The front surface of the heat preservation sleeve 1 is fixedly connected with the controller 101, the front surface of the controller 101 is rotationally connected with the temperature adjusting knob 102, the outer surface of the heat preservation sleeve 1 is wound with the resistance wire 103, and the resistance wire 103 is electrically connected with the controller 101.

[0026] In this embodiment, the controller 101 cooperates with the temperature adjusting knob 102 to control the temperature of the resistance wire 103, and further controls the temperature in the inside of the enzymolysis jar 2, wherein a PT1000 series temperature sensor is installed in the inside of the enzymolysis jar 2, for sensing the temperature in the enzymolysis jar 2 in cooperation with the controller 101.

[0027] As shown in the drawings, Figure 3As shown, the inner surface of the fixed seat 302 is fixedly connected with an L-shaped scraper 303, the outer surface of the articulated rod 304 is fixedly connected with a first stirring blade 305, and the outer surface of the articulated rod 304 is fixedly connected with a second stirring blade 306.

[0028] In this embodiment, the long edge of the scraper 303 can be used to contact the side wall of the enzymolysis tank 2, the short edge of the scraper 303 can be used to contact the bottom surface of the enzymolysis tank 2, and the first stirring blade 305 and the articulated rod 304 are used to stir the raw materials and enzymes.

[0029] As shown in the figure, Figure 2 the upper surface of the upper cover 6 is fixedly connected with a spline seat 4 near the center position, the spline seat 4 is rotatably connected with a spline shaft 401 inside, the connecting sleeve 505 penetrates through the spline shaft 401, and the connecting sleeve 505 and the spline shaft 401 are in sliding connection.

[0030] In this embodiment, the connecting sleeve 505 can move up and down inside the spline shaft 401, the hydraulic cylinder 503 is started, the output end of the hydraulic cylinder 503 drives the mounting plate 5 to rise or fall, the limiting rod 504 limits the rising and falling of the mounting plate 5, the connecting sleeve 505 drives the second articulated seat 301 to rise and fall, the distance between the second articulated seat 301 and the first articulated seat 3 changes, the angle of the articulated rod 304 changes, the fixed seat 302 further drives the scraper 303 to approach the inner wall of the enzymolysis tank 2, the inner wall of the enzymolysis tank 2 can be scraped by the scraper 303 in the later stage of enzymolysis, the inner wall of the enzymolysis tank 2 is avoided from being scraped all the time, the degree of wear of the scraper 303 is reduced, the positions of the first stirring blade 305 and the second stirring blade 306 can be adjusted, the raw materials and enzymes at different positions can be stirred, the stirring efficiency of the device is improved, and after the outer surface of the scraper 303 is worn, the unworn scraper 303 can also contact the inner wall of the enzymolysis tank 2.

[0031] As shown in the figure, Figure 2 the upper surface of the upper cover 6 is fixedly connected with a spline seat 4 near the center position, the spline seat 4 is rotatably connected with a spline shaft 401 inside, the connecting sleeve 505 penetrates through the spline shaft 401, and the connecting sleeve 505 and the spline shaft 401 are in sliding connection.

[0032] In this embodiment, the connecting sleeve 505 is rotated by the reduction box 501 in cooperation with the stepping motor 502, the spline shaft 401 makes the rotation of the connecting sleeve 505 more stable, the stepping motor 502 needs to be closed when the position of the connecting sleeve 505 is adjusted, in addition, the connecting sleeve 505 drives the auger shaft 201 and the rotating shaft 203 to rotate, the rotating shaft 203 drives the scraper 303 and the articulated rod 304 to rotate when rotating, and the auger shaft 201 facilitates the discharge of the residues after the reaction of the enzymolysis tank 2 under the conveying of the threaded blade.

[0033] Embodiment two

[0034] On the basis of embodiment one, in order to make up for the problem of inconvenient crushing of raw materials in embodiment one.

[0035] As Figure 4 shown, the upper cover 6 and the upper tank 601 are connected at the nesting connection position of the feeding valve 602, the inside of the upper cover 6 is fixedly connected with the crushing teeth 603, the upper surface of the upper cover 6 is detachably connected with the cover body 7, the upper surface of the cover body 7 is fixedly connected with the crushing motor 701, and the output end of the crushing motor 701 is fixedly connected with the crushing shaft 702.

[0036] In this embodiment, the crushing components on the crushing teeth 603 and the crushing shaft 702 are staggered, the crushing teeth 603 cooperate with the crushing shaft 702 to crush the raw materials, reduce the mixing pressure of the enzymolysis tank 2, and facilitate the unloading through the feeding valve 602.

[0037] It is apparent for those skilled in the art that the present application is not limited to the details of the above exemplary embodiments, but can be implemented in other concrete forms without departing from the spirit or essential characteristics of the present application. Therefore, the embodiments should be considered as exemplary and non-limiting, and the scope of the present application is defined by the appended claims rather than the above description, and all changes falling within the meaning and range of the equivalent elements of the claims are intended to be embraced in the present application. Any reference signs in the claims should not be considered as limiting the claims involved.

[0038] In addition, it should be understood that, although the present specification is described in terms of embodiments, not every embodiment contains only one independent technical solution, and the description manner of the specification is only for the sake of clarity, and those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be properly combined to form other embodiments that those skilled in the art can understand.

Claims

1. A preparation device for small molecule peptide enzymatic extraction, comprising an enzymolysis tank (2), characterized in that, The outer surface of the enzyme hydrolysis tank (2) is nested with a heat preservation sleeve (1), the inside of the enzyme hydrolysis tank (2) is rotatably connected with an auger shaft (201), the inside of the enzyme hydrolysis tank (2) is fixedly connected with a rotating shaft (203) at the upper end of the auger shaft (201), the upper end of the rotating shaft (203) is nested with a connecting sleeve (505), the surface of the rotating shaft (203) is fixedly connected with a first hinged seat (3), the surface of the rotating shaft (203) is movably connected with a second hinged seat (301), the surfaces of the first hinged seat (3) and the second hinged seat (301) are hingedly connected with a hinged rod (304), the other end of the hinged rod (304) is hingedly connected with a fixed seat (302), the upper surface of the enzyme hydrolysis tank (2) is fixedly connected with an upper cover (6), the upper surface of the upper cover (6) is fixedly connected with a hydraulic cylinder (503), the output end of the hydraulic cylinder (503) is fixedly connected with a mounting plate (5), the upper surface of the mounting plate (5) is fixedly connected with a speed reducer (501), and the upper surface of the upper cover (6) is fixedly connected with a feeding tank (601).

2. The device for preparing small molecule peptide enzymatic extraction according to claim 1, characterized in that, The front surface of the heat preservation sleeve (1) is fixedly connected with a controller (101), the front surface of the controller (101) is rotatably connected with a temperature adjusting knob (102), and the outer surface of the heat preservation sleeve (1) is wound with a resistance wire (103). The resistance wire (103) and the controller (101) are electrically connected.

3. The device for preparing small molecule peptide enzymatic extraction according to claim 1, characterized in that, The inner surface of the fixed seat (302) is fixedly connected with an L-shaped scraper (303), the outer surface of the hinged rod (304) is fixedly connected with a first stirring blade (305), and the outer surface of the hinged rod (304) is fixedly connected with a second stirring blade (306).

4. The device for preparing small molecule peptide enzymatic extraction according to claim 1, characterized in that, The upper surface of the upper cover (6) is fixedly connected with a spline seat (4) near the center position, the inside of the spline seat (4) is rotatably connected with a spline shaft (401), the connecting sleeve (505) penetrates through the spline shaft (401), and the connecting sleeve (505) and the spline shaft (401) are slidably connected.

5. The device for preparing small molecule peptide enzymatic extraction according to claim 1, characterized in that, The upper surface of the speed reducer (501) is fixedly connected with a stepping motor (502), the output end of the stepping motor (502) and the input end of the speed reducer (501) are fixedly connected, the output end of the speed reducer (501) and the connecting sleeve (505) are fixedly connected, the upper surface of the upper cover (6) is fixedly connected with a limiting rod (504), and the mounting plate (5) and the limiting rod (504) are slidably connected.

6. The device for preparing small molecule peptide enzymatic extraction according to claim 1, characterized in that, The connecting position of the upper cover (6) and the feeding tank (601) is nested with a feeding valve (602), and the inside of the upper cover (6) is fixedly connected with a crushing tooth (603).

7. The device for preparing a small molecule peptide enzymatic extraction according to claim 1, characterized in that, The upper surface of the upper cover (6) is detachably connected with a cover body (7), the upper surface of the cover body (7) is fixedly connected with a crushing motor (701), and the output end of the crushing motor (701) is fixedly connected with a crushing shaft (702).

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