Batch dyeing device for transmission electron microscope samples

Abstract

The utility model discloses a transmission electron microscope sample batch dyeing device which comprises a supporting frame, a dyeing mechanism is arranged on the inner side of the supporting frame, a transmission mechanism is arranged above the dyeing mechanism, and the dyeing mechanism comprises a cleaning liquid pool, a first dyeing liquid pool, a second dyeing liquid pool and an eluent pool. A cleaning solution pool, a first staining solution pool, a second staining solution pool and an eluent pool are distributed on the inner side of the supporting frame from left to right, transmission rods and turbines are arranged in the cleaning solution pool, the first staining solution pool, the second staining solution pool and the eluent pool, and the transmission mechanism comprises a mounting plate, a magnetic attraction block, a fixing plate, a placement net box and a vibration motor. A magnetic attraction block is fixedly mounted at the front end of the mounting plate, a fixing plate is arranged in front of the magnetic attraction block, an iron block is mounted in the fixing plate, and a placement net box is fixedly mounted at the front end of the fixing plate. According to the transmission electron microscope sample batch dyeing device, the dyeing mechanism and the transmission mechanism work cooperatively, and the consistency, efficiency and repeatability of sample dyeing are remarkably improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to transmission electron microscope sample processing field, specifically a transmission electron microscope sample batch dyeing device. BACKGROUND

[0002] Transmission electron microscope sample dyeing is after ultrathin section preparation, immerging or point dyeing heavy metal solution containing osmium salt, uranium acetate, lead citrate, phosphotungstic acid, makes these dyeing agent selective adhesion in lipid, protein, nucleic acid etc. area, forms heavy metal layer of different thickness and density. In this way, when electron beam passes, heavy metal area scatters stronger, image contrast greatly promotes, needs rinsing, dehydration, drying and low temperature fixation after dyeing to remove redundant dyeing solution and protect structure, to keep sample stable, often adjust pH value in buffer solution, and control dyeing concentration and time according to gradient, in key parts such as organelle, can adopt uranium and lead double dyeing.

[0003] Traditional transmission electron microscope sample dyeing process usually relies on technical personnel to repeatedly drop dyeing, scrape redundant dyeing solution and natural drying, this mode not only requires high to operating personnel, is great in labor intensity, is inefficient, and because of difficult accurate control dyeing solution dosage and coating uniformity, often appears that dyeing is not the same, consistency is poor, in addition, liquid distribution is uneven in dyeing pool, especially pool bottom often forms concentration gradient because of reagent precipitation and accumulation, further interferes with dyeing effect and increases operation difficulty, thereby seriously restricts the repeatability and observation quality of experiment, based on this, the utility model designs a transmission electron microscope sample batch dyeing device to solve the above -mentioned problems. UTILITY MODEL CONTENT

[0004] The utility model is to provide a transmission electron microscope sample batch dyeing device to solve the problems in the background art.

[0005] To achieve the above object, the utility model provides the following technical scheme:

[0006] A transmission electron microscope sample batch dyeing device, including support frame, the inside of support frame is provided with dyeing mechanism, the top of dyeing mechanism is provided with transmission mechanism, dyeing mechanism includes cleaning liquid pool, first dyeing liquid pool, second dyeing liquid pool and eluent pool, the inside of support frame is distributed with cleaning liquid pool, first dyeing liquid pool, second dyeing liquid pool and eluent pool from left to right respectively, the inside of cleaning liquid pool, first dyeing liquid pool, second dyeing liquid pool and eluent pool is provided with transmission rod and turbine, transmission mechanism includes mounting plate, magnetic attraction piece, fixed plate, deposit net box and vibration motor, the front end of mounting plate is fixedly installed with magnetic attraction piece, the front of magnetic attraction piece is provided with fixed plate, the inside of fixed plate is installed with iron block, the front end of fixed plate is fixedly installed with deposit net box, the middle part of mounting plate is fixedly installed with vibration motor.

[0007] Optionally, the inside of the cleaning liquid pool is fixedly installed with a first spray head, the front end of the first spray head is fixedly connected with a first liquid delivery pipe, and the end, away from the first spray head, of the first liquid delivery pipe is fixedly connected with a first liquid pump.

[0008] Optionally, the inside of the elution liquid pool is fixedly installed with a second spray head, the front end of the second spray head is fixedly connected with a second liquid delivery pipe, and the end, away from the second spray head, of the second liquid delivery pipe is fixedly connected with a second liquid pump.

[0009] Optionally, the inner walls of the cleaning liquid pool, the first dyeing liquid pool, the second dyeing liquid pool and the elution liquid pool are all fixedly installed with bearing seats, the middle parts of the bearing seats are installed with transmission rods, and the lower ends of the transmission rods are fixedly installed with turbines.

[0010] Optionally, the upper ends of the transmission rods are fixedly installed with pulleys, the outer sides of the pulleys are provided with transmission belts, the front ends of the transmission belts are provided with driving wheels, the lower parts of the driving wheels are provided with driving motors, the transmission ends of the driving motors are fixedly connected with the driving wheels, the driving motors are fixedly installed at the front ends of supporting frames, and the transmission belts are engaged with the pulleys, so that the turbines driven by the transmission rods rotate synchronously to promote the circulation of the liquid in the pools.

[0011] Optionally, the rear end of the mounting plate is fixedly installed with a first ball screw nut seat, the inside of the first ball screw nut seat is provided with a first ball screw rod, the rear part of the first ball screw rod is provided with a first loading plate, the upper end of the first loading plate is fixedly installed with a first stepping motor, the transmission end of the first stepping motor is connected with the first ball screw rod, and the left and right ends of the first loading plate are both fixedly installed with first limiting rails in limiting relationship with the mounting plate.

[0012] Optionally, the rear end of the first loading plate is fixedly installed with a second ball screw nut seat, the inside of the second ball screw nut seat is provided with a second ball screw rod, the left and right sides of the second ball screw rod are both provided with second loading plates, the outer wall of the second loading plate is fixedly installed with a second stepping motor, the transmission end of the second stepping motor is connected with the second ball screw rod, the upper and lower parts of the second ball screw rod are both provided with second limiting rails in limiting relationship with the first loading plate.

[0013] Compared with the prior art, the utility model has the advantages that:

[0014] 1. The utility model discloses a dyeing mechanism is provided with, through the gradient type soaking and the spray cooperation of vibration motor and liquid pump circulation effect of cleaning liquid pool, first dyeing liquid pool, second dyeing liquid pool and eluent pool, realize sample surface impurity thorough removal and dyeing fluid high efficiency, even cover, and through the synchronous drive four liquid pools liquid circulation of drive motor, transmission belt and turbine, guarantee liquid mixing even.

[0015] 2. The utility model discloses a transmission mechanism is provided with, utilize the horizontal and vertical movement of placing net box of ball screw, stepping motor accurate control, guarantee operation stable and reliable. ACCURACY

[0016] Figure 1 It is the structure schematic diagram of the utility model three -dimensional front view;

[0017] Figure 2 It is the structure schematic diagram of the utility model three -dimensional plan view;

[0018] Figure 3 It is the structure schematic diagram of the utility model plane front view;

[0019] Figure 4 It is the structure schematic diagram of the utility model three -dimensional rear view;

[0020] Figure 5 It is the structure schematic diagram of the utility model plane plan view;

[0021] Figure 6 It is the structure schematic diagram of the utility model three -dimensional section view Figure 1 ;

[0022] Figure 7 It is the structure schematic diagram of the utility model three -dimensional section view Figure 2 ;

[0023] Figure 8 It is the structure schematic diagram of the utility model three -dimensional section view Figure 3 ;

[0024] Figure 9 It is the structure schematic diagram of the utility model Figure 8 A place three -dimensional enlarged structure schematic drawing;

[0025] Figure 10 It is the structure schematic diagram of the utility model three -dimensional section view Figure 4 .

[0026] In the figure: 1, support frame; 2, dyeing mechanism; 201, cleaning liquid pool; 202, first dyeing liquid pool; 203, second dyeing liquid pool; 204, eluent pool; 205, first spray head; 206, first liquid delivery pipe; 207, first liquid pump; 208, bearing seat; 209, transmission rod; 210, turbine; 211, pulley; 212, transmission belt; 213, drive wheel; 214, drive motor; 215, second spray head; 216, second liquid delivery pipe; 217, second liquid pump; 3, transmission mechanism; 301, mounting plate; 302, magnetic block; 303, fixed plate; 304, placed net box; 305, vibration motor; 306, first ball nut seat; 307, first ball screw; 308, first loading plate; 309, first stepping motor; 310, first limit rail; 311, second ball nut seat; 312, second ball screw; 313, second loading plate; 314, second stepping motor; 315, second limit rail. DETAILED DESCRIPTION

[0027] In the description of the present application, it is to be understood that the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first", "second" and the like are only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features limited by "first", "second" and the like can explicitly or implicitly include one or more of the features. In the description of the present application, unless otherwise specified, the meaning of "a plurality of" is two or more.

[0028] In the description of the present application, it should be noted that, unless otherwise specified and limited, the terms "mounting", "connection", "connection" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be the communication between two elements. For ordinary skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0029] Clearly, the described embodiments are merely a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0030] Please refer to Figures 1-10 In the embodiment of the present application, a transmission electron microscope sample batch staining device comprises a support frame 1, a staining mechanism 2 is arranged on the inner side of the support frame 1, a transmission mechanism 3 is arranged above the staining mechanism 2, the staining mechanism 2 comprises a cleaning liquid pool 201, a first staining liquid pool 202, a second staining liquid pool 203 and an eluent pool 204, the cleaning liquid pool 201, the first staining liquid pool 202, the second staining liquid pool 203 and the eluent pool 204 are distributed from left to right on the inner side of the support frame 1, transmission rods 209 and turbines 210 are arranged in the cleaning liquid pool 201, the first staining liquid pool 202, the second staining liquid pool 203 and the eluent pool 204, the transmission mechanism 3 comprises a mounting plate 301, a magnetic block 302, a fixed plate 303, a placing mesh box 304 and a vibration motor 305, the magnetic block 302 is fixedly installed at the front end of the mounting plate 301, the fixed plate 303 is arranged in front of the magnetic block 302, an iron block is installed in the fixed plate 303, the placing mesh box 304 is fixedly installed at the front end of the fixed plate 303, and the vibration motor 305 is fixedly installed at the middle part of the mounting plate 301.

[0031] The cleaning liquid pool 201, the first staining liquid pool 202, the second staining liquid pool 203 and the eluent pool 204 constitute a staining assembly, the bearing seat 208, the transmission rod 209, the turbine 210, the belt wheel 211, the transmission belt 212, the driving wheel 213 and the driving motor 214 constitute a mixing assembly; the mounting plate 301, the magnetic block 302, the fixed plate 303, the placing mesh box 304 and the vibration motor 305 constitute an assembly assembly, the first ball nut seat 306, the first ball screw 307, the first loading plate 308, the first stepping motor 309, the first limiting rail 310, the second ball nut seat 311, the second ball screw 312, the second loading plate 313, the second stepping motor 314 and the second limiting rail 315 constitute a transmission assembly.

[0032] The device main body is provided with the support frame 1 for fixing and supporting the staining mechanism 2 and the transmission mechanism 3. The staining mechanism 2 is sequentially provided with the cleaning liquid pool 201, the first staining liquid pool 202, the second staining liquid pool 203 and the eluent pool 204 along the horizontal direction, each liquid pool is filled with corresponding liquid and is provided with a circulating stirring part and a spraying system. The transmission mechanism 3 is composed of an assembly assembly and a transmission assembly, is responsible for the positioning movement of the placing mesh box 304 and the circulating stirring of the liquid in each liquid pool.

[0033] The structure and working process of the dyeing mechanism 2:

[0034] A bearing seat 208 is fixed on the upper part of the inner wall of each liquid pool, a transmission rod 209 is installed through the middle part of the bearing seat 208, a turbine 210 is fixed on the lower end of the transmission rod 209, and the turbine 210 is placed in the liquid and synchronously rotates driven by the mixing assembly to form liquid circulation.

[0035] High-purity distilled water is filled in the cleaning liquid pool 201, the first spray head 205 is fixed above the liquid pool, the front end of the spray head is connected with the first liquid conveying pipe 206, and the other end of the first liquid conveying pipe is connected with the first liquid pump 207. When working, the net box 304 is immersed in the cleaning liquid pool 201, the first liquid pump 207 is started, distilled water is sprayed on the sample surface through the first liquid conveying pipe 206, the turbine 210 is stirred, and the vibration motor 305 is oscillated, so that dust, residual fixing agent and salt on the sample surface are completely removed.

[0036] Heavy metal dyeing liquid is filled in the first dyeing liquid pool 202, after the net box 304 is immersed, the spraying system is not needed, and only the turbine 210 is stirred and the vibration motor 305 is oscillated, so that the dyeing liquid fully contacts the sample surface to realize preliminary negative dyeing contrast enhancement.

[0037] Secondary heavy metal dyeing liquid is filled in the second dyeing liquid pool 203, and the same immersion, oscillation and stirring mode as the first dyeing liquid pool 202 is adopted, so that the dyeing liquid is further deposited on the sample film structure to improve the electron density difference between the film and the tissue interface.

[0038] Deionized water is filled in the elution liquid pool 204, the second spray head 215, the second liquid conveying pipe 216 and the second liquid pump 217 form a spraying system. After the net box 304 is immersed, the second liquid pump 217 is started, deionized water is sprayed on the sample surface through the second liquid conveying pipe 216, and the turbine 210 is circularly stirred to completely wash and remove unbound dyeing liquid and ensure clean background.

[0039] The structure and action process of the transmission mechanism 3:

[0040] The assembly components include a mounting plate 301, a magnetic block 302, a fixed plate 303, a net box 304 and a vibration motor 305. The magnetic block 302 is fixed on the front end of the mounting plate 301, the fixed plate 303 is arranged in front of the magnetic block 302, the fixed plate 303 is internally provided with an iron block, and the magnetic block 302 cooperates with the iron block to realize one-key type quick docking of the net box 304 and the mounting plate 301. The vibration motor 305 is installed in the middle part of the mounting plate 301, micro-oscillation is transmitted to the net box 304 through a vibration rod, and the liquid surface update rate is improved.

[0041] The transmission assembly is composed of the first ball nut seat 306, the first ball screw 307, the first loading plate 308, the first stepping motor 309, the first limiting rail 310, the second ball nut seat 311, the second ball screw 312, the second loading plate 313, the second stepping motor 314, the second limiting rail 315, the belt pulley 211, the transmission belt 212, the driving wheel 213, the driving motor 214, the transmission rod 209 and the turbine 210. The second stepping motor 314 drives the second ball screw 312 to rotate, drives the second ball nut seat 311 and the first loading plate 308 to move horizontally on the first limiting rail 310 and the second limiting rail 315, and positions the net box 304 above each liquid pool; the first stepping motor 309 drives the first ball screw 307 to rotate, drives the first ball nut seat 306 and the assembly to ascend and descend along the vertical direction, and realizes the up and down sinking of the net box 304; the driving motor 214 drives the driving wheel 213 to rotate, the transmission belt 212 is engaged with the belt pulley 211, drives each transmission rod 209 and the turbine 210 to rotate synchronously, and promotes the continuous circulation and stirring of the liquid in the liquid pool.

[0042] The cooperative work flow is as follows:

[0043] The operator places the sample into the net box 304, completes the rapid fixing by the magnetic block 302 and the fixed plate 303, starts the second stepping motor 314, and positions the net box 304 from left to right above the cleaning liquid pool 201, the first dyeing liquid pool 202, the second dyeing liquid pool 203 and the elution liquid pool 204 in sequence; at each liquid pool position, the first stepping motor 309 controls the sinking and lifting of the net box 304, the vibration motor 305 cooperates with the mixing assembly, cooperates with the spraying system of the first liquid pump 207 or the second liquid pump 217, and sequentially completes the cleaning, the dyeing, the secondary heavy metal dyeing and the elution; after the dyeing is completed, the net box 304 is lifted by the first stepping motor 309, and is moved back to the initial position by the second stepping motor 314, and the sample is taken out by the personnel.

[0044] The embodiment realizes the full-flow automatic cleaning, dyeing, secondary dyeing and elution of the transmission electron microscope sample by the accurate control of the spatial movement and vibration of the net box 304 and the cooperation of the multi-stage spraying and circulation stirring, effectively avoids the interference of the liquid precipitation and the concentration gradient on the dyeing effect, and significantly improves the consistency, efficiency and repeatability of the sample dyeing.

[0045] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A transmission electron microscope sample batch staining device comprising a support frame (1), characterized in that: The inner side of the support frame (1) is provided with a dyeing mechanism (2), the upper side of the dyeing mechanism (2) is provided with a transmission mechanism (3), the dyeing mechanism (2) comprises a cleaning liquid pool (201), a first dyeing liquid pool (202), a second dyeing liquid pool (203) and an eluent pool (204), the inner side of the support frame (1) is distributed with the cleaning liquid pool (201), the first dyeing liquid pool (202), the second dyeing liquid pool (203) and the eluent pool (204) from left to right, the inside of the cleaning liquid pool (201), the first dyeing liquid pool (202), the second dyeing liquid pool (203) and the eluent pool (204) is provided with a transmission rod (209) and a turbine (210), the transmission mechanism (3) comprises a mounting plate (301), a magnetic block (302), a fixed plate (303), a placing net box (304) and a vibration motor (305), the front end of the mounting plate (301) is fixedly provided with the magnetic block (302), the front side of the magnetic block (302) is provided with the fixed plate (303), the inside of the fixed plate (303) is provided with an iron block, the front end of the fixed plate (303) is fixedly provided with the placing net box (304), the middle part of the mounting plate (301) is fixedly provided with the vibration motor (305). 2.The transmission electron microscope sample batch staining device according to claim 1, characterized in that: The inside of the cleaning liquid pool (201) is fixedly provided with a first spray head (205), the front end of the first spray head (205) is fixedly connected with a first liquid conveying pipe (206), one end of the first liquid conveying pipe (206) away from the first spray head (205) is fixedly connected with a first liquid pump (207). 3.The transmission electron microscope sample batch staining device according to claim 1, wherein: The inside of the eluent pool (204) is fixedly provided with a second spray head (215), the front end of the second spray head (215) is fixedly connected with a second liquid conveying pipe (216), one end of the second liquid conveying pipe (216) away from the second spray head (215) is fixedly connected with a second liquid pump (217). 4.The transmission electron microscope sample batch staining device according to claim 1, wherein: The inner wall of the cleaning liquid pool (201), the first dyeing liquid pool (202), the second dyeing liquid pool (203) and the eluent pool (204) is fixedly provided with a bearing seat (208), the middle part of the bearing seat (208) is provided with a transmission rod (209), the lower end of the transmission rod (209) is fixedly provided with a turbine (210).

5. The transmission electron microscope sample batch staining device of claim 4, wherein: The upper end of each transmission rod (209) is fixedly provided with a pulley (211), the outer side of the pulley (211) is provided with a transmission belt (212), the front end of the transmission belt (212) is provided with a driving wheel (213), the lower side of the driving wheel (213) is provided with a driving motor (214), the transmission end of the driving motor (214) is fixedly connected with the driving wheel (213), the driving motor (214) is fixedly installed at the front end of the support frame (1), the transmission belt (212) is engaged with the pulley (211), so that the turbines (210) driven by the transmission rods (209) rotate synchronously, so as to promote the circulation of the liquid in the pool. 6.The transmission electron microscope sample batch staining device according to claim 1, wherein: The rear end of the mounting plate (301) is fixedly installed with a first ball nut seat (306), the inside of the first ball nut seat (306) is provided with a first ball screw (307), the rear of the first ball screw (307) is provided with a first loading plate (308), the upper end of the first loading plate (308) is fixedly installed with a first stepping motor (309), the transmission end of the first stepping motor (309) is connected with the first ball screw (307), the left and right ends of the first loading plate (308) are both fixedly installed with a first limiting rail (310), and the first limiting rail (310) is in limiting relationship with the mounting plate (301).

7. The transmission electron microscope sample batch staining device of claim 6, wherein: The rear end of the first loading plate (308) is fixedly installed with a second ball nut seat (311), the inside of the second ball nut seat (311) is provided with a second ball screw (312), the left and right sides of the second ball screw (312) are both provided with a second loading plate (313), the outer wall of the second loading plate (313) is fixedly installed with a second stepping motor (314), the transmission end of the second stepping motor (314) is connected with the second ball screw (312), the upper and lower sides of the second ball screw (312) are both provided with a second limiting rail (315), and the second limiting rail (315) is in limiting relationship with the first loading plate (308).

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