Biological sample nucleic acid extraction treatment system

Abstract

The utility model relates to the technical field of biochemical experiment automation equipment, and discloses a biological sample nucleic acid extraction processing system, which comprises a rack and a control unit, a working platform is arranged in the rack, a mechanical arm is arranged above the rack, a module unit is arranged on the working platform, and the control unit is connected with the mechanical arm. Comprising a sample tube plate frame, a suction head frame, a suction head recycling box, a pore plate frame, a low-temperature incubation unit, a low-temperature swing unit, a tube plate opening and closing cover unit, a low-speed centrifugal unit, a heating unit, a magnetic frame unit, a PCR unit and a fluorescence detection unit, the sample tube plate frame is arranged in the center of a workbench, and other unit modules are arranged around the sample tube frame; a clamping jaw and a liquid injection unit are arranged on the mechanical arm, and the control unit controls the mechanical arm and each module unit to act, so that a nucleic acid extraction treatment flow is realized. According to the utility model, branch processes are completed through each module unit, the control unit connects the processes in series to complete nucleic acid extraction, and various processes are realized in a self-defined manner.

Description

TECHNICAL FIELD

[0001] The utility model relates to biochemical experiment automation equipment technical field especially, it relates to a biological sample nucleic acid extraction processing system. BACKGROUND

[0002] The biological sample carries out nucleic acid extraction experiment, the process is complex, and the operation and detection time are long, for the condition that the sample quantity is many, often more staff participates, and the time -consuming is long, and the precision is low. Taking the nucleic acid extraction of animal and plant cells as an example, often a process is realized, and it takes ten days or more days to complete.

[0003] With the development of laboratory automation technology, the nucleic acid detection process is realized through the automation platform, and has become a reality. Then, this automation process is mainly to realize a plurality of related sub-processes respectively, and then is combined into a complete process work line. This form can be better realized for a single experimental process. However, for different samples, the process is slightly different, and the entire laboratory automation equipment needs to be modified and replaced. For multi-process experiments, the requirement cannot be met. UTILITY MODEL CONTENT

[0004] The utility model aims at solving above -mentioned problem, provides a biological sample nucleic acid extraction processing system, completes branch process through each module unit, and the control unit completes nucleic acid extraction by process concatenation, and realizes self -definition for a variety of processes.

[0005] The technical scheme adopted by the utility model is:

[0006] A biological sample nucleic acid extraction processing system is characterized by comprising a rack and a control unit, a work platform is arranged in the rack, a mechanical arm is arranged above the rack, a module unit is arranged on the work platform, and the module unit comprises

[0007] A sample tube plate rack, a suction head rack, a suction head recycling box, a hole plate rack, a low-temperature incubation unit, a low-temperature swing unit, a tube plate opening and closing cover unit, a low-speed centrifugation unit, a heating unit, a magnetic rack unit, a PCR unit and a fluorescence detection unit,

[0008] The sample tube plate rack is arranged at the central position of the workbench, and other unit modules are arranged around the sample tube rack,

[0009] A clamping jaw and a liquid injection unit are arranged on the mechanical arm,

[0010] The sample tube plate rack is used for placing a sample tube plate, the tube plate clamping jaw is used for picking and placing the sample tube plate, and the sample tube is placed on the tube plate;

[0011] The liquid injection unit completes liquid treatment in the sample tube through the loading and unloading of the suction head;

[0012] The tip recycling box is used for collecting unloaded tips and waste liquid collection;

[0013] The well plate rack is used for liquid separation of sample tubes;

[0014] The tube plate opening and closing cover unit is used for opening and closing the sample tubes on the tube plate;

[0015] The magnetic frame unit is used for adsorbing magnetic beads, and the magnetic beads are combined with nucleic acid substances;

[0016] The PCR unit and the fluorescence detection unit realize amplification and quantification of nucleic acid substances,

[0017] The control unit controls the mechanical arm and each module unit to act, realizing the nucleic acid extraction process.

[0018] Further, the module unit further comprises a shock breaking unit and a high-speed centrifugal unit, which are arranged outside other module units, the shock breaking unit is used for breaking biological samples to obtain nucleic acid substances, and the high-speed centrifugal unit is used for centrifugal separation of the broken sample.

[0019] Further, the sample tube plate rack is provided with a sample tube plate, the sample tube plate is provided with four channels for placing four sample tubes, the sample tube is a centrifugal tube, and the end of the sample tube plate is provided with a positioning mounting hole which is commonly mounted on each module unit.

[0020] Further, the working platform is provided with a front and rear direction clamping block array, the base of each module unit is provided with a clamping groove, and each module unit is sequentially and multi-row clamped on the working platform through the clamping block and the clamping groove.

[0021] Further, the sample tube plate rack, the tip rack and the well plate rack are multiple and arranged in one row or multiple rows, the tip recycling box is arranged outside the tip rack, and other module units are arranged in one row or multiple rows in the periphery.

[0022] Further, the sample tube plate rack is provided with multiple sample tube plates, the tip rack is provided with a tip array, the tip array comprises multiple specifications of tips, the well plate rack is provided with a well plate, the clamping jaw is used for taking and placing the sample tube plate and the well plate, and the liquid injection unit is a four-channel pipettor.

[0023] Further, the low-temperature incubation unit comprises

[0024] A low-temperature module and a container rack mounted on the low-temperature module, the container rack corresponds to multiple specifications of containers;

[0025] The low-temperature swing unit comprises a low-temperature module and a swing module, and the swing module drives the low-temperature module and a sample tube plate on the low-temperature module to swing and mix;

[0026] The tube plate opening and closing cover unit comprises a tube plate seat and an opening and closing pressing plate, and the sample tube on the tube plate seat is opened and closed through the overturning action of the pressing plate.

[0027] The low-speed centrifugal unit comprises a tube plate seat and a centrifugal motor, the tube plate seat is symmetrically arranged in two, and the centrifugal motor drives the tube plate seat to rotate slowly to realize centrifugation.

[0028] The heating unit comprises a tube plate seat and an auxiliary heating table, a heating plate is arranged on the tube plate seat, the sample tube is heated, and the auxiliary heating table is arranged on the heating plate to perform heat preservation.

[0029] The magnetic frame unit comprises a magnet seat arranged in the frame, the magnet seat is provided with a magnet, a tube plate is arranged on the frame, and the sample tube on the tube plate is correspondingly matched with the magnet to realize the adsorption of the magnetic beads in the sample tube to the tube wall.

[0030] Further, the oscillation and crushing unit comprises a lifting seat and a driving motor, the driving motor drives the lifting seat to oscillate up and down through a crank wheel, the sample tube in the lifting seat is provided with a biological sample and a rigid particle, and the high-speed oscillation of the lifting seat makes the rigid particle crush the biological sample.

[0031] The utility model discloses the beneficial effect is:

[0032] (1) cover the nucleic acid detection experiment process about sample processing, liquid processing, nucleic acid extraction, amplification detection complete technology, provide the guarantee for the whole process of nucleic acid detection;

[0033] (2) after the process analysis of the control unit, each process combination is formed into a complete detection process;

[0034] (3) suitable for various nucleic acid detection processes for biological cells. BRIEF DESCRIPTION OF DRAWINGS

[0035] ATTACH Figure 1 It is the whole three-dimensional structure schematic diagram of the utility model;

[0036] ATTACH Figure 2 It is the arrangement schematic diagram of module unit;

[0037] ATTACH Figure 3 It is the local enlarged view of work platform clamping structure;

[0038] ATTACH Figure 4 It is the local enlarged view of sample tube plate frame arrangement;

[0039] ATTACH Figure 5 It is the structure schematic diagram of sample tube plate;

[0040] ATTACH Figure 6 It is the local enlarged view of suction head frame and hole plate frame arrangement.

[0041] Figure 2 is a partial enlarged view of the mechanical arm inside the rack; Figure 7

[0042] Figure 3 is a partial enlarged view of the clamping jaw; Figure 8

[0043] Figure 4 is a partial enlarged view of the tube plate opening and closing cover unit; Figure 9

[0044] Figure 5 is a partial enlarged view of the magnetic frame unit; Figure 10

[0045] Figure 6 is a partial enlarged view of the oscillation and crushing unit; Figure 11

[0046] Figure 7 is a partial enlarged view of the low-temperature incubation unit; Figure 12

[0047] Figure 8 is a partial enlarged view of the low-temperature swing unit; Figure 13

[0048] Figure 9 is a partial enlarged view of the low-speed centrifugation unit; Figure 14

[0049] Figure 10 is a partial enlarged view of the heating unit; Figure 15

[0050] Figure 11 is a partial enlarged view of the PCR unit and fluorescence detection unit. Figure 16

[0051] The reference signs in the drawings are as follows:

[0052] 1. Rack; 2. Workbench;

[0053] 3. Mechanical arm; 4. Module unit;

[0054] 5. Sample tube plate rack; 6. Suction head rack;

[0055] 7. Suction head recovery box; 8. Hole plate rack;

[0056] 9. Low-temperature incubation unit; 10. Low-temperature swing unit;

[0057] 11. Tube plate opening and closing cover unit; 12. Low-speed centrifugation unit;

[0058] 13. Heating unit; 14. Magnetic frame unit;

[0059] 15. PCR unit; 16. Fluorescence detection unit;

[0060] 17. Block array; 18. Sample tube plate;

[0061] ​​​​​​​​​​19. Positioning mounting hole; 20. Tube plate seat;

[0062] 21. Opening and closing pressing plate; 22. Magnet seat;

[0063] 23. Magnet; 24. Frame;

[0064] 25. Oscillation crushing unit; 26. High-speed centrifugal unit;

[0065] 27. Lifting seat; 28. Driving motor;

[0066] 29. Crank wheel; 30. Low-temperature module;

[0067] 31. Container rack; 32. Low-temperature module;

[0068] 33. Swing module; 34. Tube plate seat;

[0069] 35. Tube plate seat; 36. Auxiliary heating table;

[0070] 37. Hot pressing plate; 38. X-axis;

[0071] 39. Y-axis; 40. Liquid injection unit;

[0072] 41. Clamping jaw. DETAILED DESCRIPTION

[0073] The specific embodiments of the biological sample nucleic acid extraction processing system of the utility model will be described in detail below in combination with the drawings.

[0074] Referring to the drawings, Figure 1 The biological sample nucleic acid extraction processing system of the utility model comprises a rack 1 and a control unit, a workbench 2 is arranged in the rack 1, a mechanical arm 3 is arranged above the rack 1, and a module unit 4 is arranged on the workbench 2. After the control unit sets a flow program, the mechanical arm 3 and each module unit 4 realize the flow experiment process. The staff only needs to provide various sample solutions used in the experiment process to the predetermined module unit 4.

[0075] Referring to the drawings, Figure 2 The module unit 4 mainly comprises a sample tube plate rack 5, a suction head rack 6, a suction head recovery box 7, a hole plate rack 8, a low-temperature incubation unit 9, a low-temperature swing unit 10, a tube plate opening and closing cover unit 11, a low-speed centrifugal unit 12, a heating unit 13, a magnetic rack unit 14, a PCR unit 15 and a fluorescence detection unit 16 and the like.

[0076] According to the use amount of the sample and the use amount of the consumables in the experiment process, the sample tubes and the suction heads and the like consumables on the sample tube plate rack 5 are pre-arranged.

[0077] Referring to the drawings, Figure 3The module units 4 in the processing system are installed on the working platform 2, and the working platform 2 is provided with a card block array 17 in the front-rear direction, and the base of each module unit 4 is provided with a clamping groove, and each module unit 4 is clamped on the working platform 2 in multiple rows through the card block and the clamping groove. The clamping mode can conveniently change the arrangement of each module unit 4, and provide the most economical working line for a specific nucleic acid detection process.

[0078] Referring to the accompanying drawings Figure 4 , 5 The sample tube rack 5 is arranged at the central position of the working platform 2, and other unit modules are arranged around the sample tube rack 5. The sample tube rack 5 is provided with multiple rows of sample tube plates 18, and each sample tube plate 18 is provided with four channels for placing four sample tubes. The sample tube plate 18 is provided with a positioning installation hole 19 at the end, which is commonly installed on each module unit 4.

[0079] The sample tube rack 5 on the working platform 2 can be multiple, which can be arranged side by side or separately in multiple places of the platform.

[0080] Referring to the accompanying drawings Figure 6 The suction head rack 6 is multiple, which is mainly arranged outside the sample tube rack 5, and the suction head rack 6 is further provided with a suction head recycling box 7 outside. The suction head rack 6 is provided with a suction head array, which includes multiple specifications of suction heads for liquid operation of different sample containers. The suction head recycling box is used for recycling used suction heads and collecting waste liquid.

[0081] The well plate rack 8 is multiple, which is distributed at multiple positions outside the sample tube rack 5, and the well plate rack 8 is provided with a well plate to meet the liquid separation in the nucleic acid detection pretreatment process.

[0082] Referring to the accompanying drawings Figure 7 , 8 The mechanical arm 3 is provided with a clamping jaw 41 and a liquid injection unit 40. The clamping jaw 41 is used for taking and placing the sample tube plate 18, the sample tube and the well plate. The clamping jaw 41 has multiple gripping points in structure, which can grip containers and materials of different sizes respectively.

[0083] The liquid injection unit 40 is a four-channel pipettor, which corresponds to the positions of the suction heads in the suction head rack 6 and the positions of the sample tubes in the sample tube rack 5. The four-channel pipettor can load four suction heads at a time, and can simultaneously process samples in four sample tubes to perform pipetting, blowing and sucking actions.

[0084] The mechanical arm 3 adopts a cross-type X-axis 38 located at the front and rear ends of the rack 1, and a Y-axis 39 is arranged on the X-axis 38. The liquid injection unit 40 and the clamping jaw 41 arranged on the Y-axis 39 can cover all module units 4 of the working platform 2.

[0085] Referring to the accompanying drawings Figure 9, the tube plate opening and closing unit 11 is used for opening and closing the sample tube on the tube plate, and the tube plate opening and closing unit 11 comprises a tube plate seat 20 and an opening and closing pressing plate 21. The opening and closing pressing plate 21 is turned over to open and close the sample tube on the tube plate seat. The turning over action of the opening and closing pressing plate 21 is realized by the movement guide path of the opening and closing pressing plate 21. According to the opening principle of the centrifugal tube, the pressing plate realizes the opening and closing action.

[0086] Referring to the accompanying drawings Figure 10 , the magnetic frame unit 14 is used for adsorbing magnetic beads, the magnetic beads have combined with nucleic acid substances in the previous operation, the magnetic frame unit 14 comprises a magnet seat 22 arranged in the frame, the magnet seat 22 is provided with a magnet 23, and the sample tube plate 18 is arranged on the frame 24. The sample tube of the sample tube plate 18 is correspondingly matched with the magnet 23 to realize adsorption of the magnetic beads in the sample tube to the tube wall.

[0087] Referring to the accompanying drawings Figure 11 , the module unit 4 is further provided with a shaking and crushing unit 25 and a high-speed centrifugal unit 26. In the experiment without providing nucleic acid samples, the cell is directly pretreated, the shaking and crushing unit 25 is used for crushing the biological sample to obtain nucleic acid substances, and the high-speed centrifugal unit 26 is used for centrifugal separation of the crushed sample. The shaking and crushing unit 25 comprises a lifting seat 27 and a driving motor 28. The driving motor 28 drives the lifting seat 27 to shake up and down through a crank wheel 29. The sample tube in the lifting seat 27 is provided with a biological sample and a rigid particle. The high-speed shaking of the lifting seat makes the rigid particle crush the biological sample.

[0088] Referring to the accompanying drawings Figure 12 , the low-temperature incubation unit 9 comprises a low-temperature module 30 and a container rack 31 arranged on the low-temperature module 30. The container rack 31 corresponds to containers of various specifications. The low-temperature incubation unit 9 can be multiple, and is respectively used for different specifications of containers, such as 1ml and 5ml specifications of centrifugal tubes.

[0089] Referring to the accompanying drawings Figure 13 , the low-temperature swinging unit 10 comprises a low-temperature module 32 and a swinging module 33. The swinging module 33 drives the low-temperature module 32 and the sample tube plate 18 on the low-temperature module 32 to swing and mix. The low-temperature swinging unit 10 realizes shaking of the sample in a low-temperature environment.

[0090] Referring to the accompanying drawings Figure 14 , the low-speed centrifugal unit 12 comprises a tube plate seat 34 and a centrifugal motor. The tube plate seat 34 is symmetrically arranged in two parts. The centrifugal motor drives the tube plate seat to rotate slowly to realize centrifugation. The tube plate seat 34 can also swing freely to achieve the effect of swinging the tube as in manual operation, so that the vapor and liquid in the tube fall back.

[0091] Referring to the accompanying drawings Figure 15The heating unit 13 includes a tube sheet seat 35 and an auxiliary heating stage 36. The heating plate on the tube sheet seat 35 heats the sample tube, and the auxiliary heating stage 36 presses the heated pressing plate 37 onto the heating plate for heat preservation.

[0092] See appendix Figure 16 The PCR unit 15 and the fluorescence detection unit 16 realize the amplification and quantification of nucleic acid substances. Both of them use existing products. The device is controlled by the control unit and the measurement data is fed back to the system.

[0093] This processing system can complete the following processes:

[0094] (1) After the biological sample is broken by the oscillating and breaking unit 25, it is centrifuged by the high-speed centrifugation unit 26, and the supernatant is drawn by the liquid injection unit and sent to the sample tube on the sample tube plate rack 5.

[0095] (2) The gripper removes the sample tube sheet 18 and sends it to the tube sheet opening and closing unit 11. The tube sheet opening and closing unit 11 opens the tube sheet, and the liquid injection unit performs one of the following on the sample tube on the sample tube sheet 18: liquid injection, liquid aspiration, or blowing and aspiration to mix. Then the tube sheet opening and closing unit 11 closes the sample tube, and the gripper outputs the sample tube sheet 18.

[0096] (3) The gripper delivers the sample tube sheet 18 to the low temperature incubation unit 9 and the heating unit 13 for cooling or heating operations. After completion, the gripper outputs the sample tube sheet 18.

[0097] (4) The liquid injection unit removes the pipette tip from the pipette tip holder 6, performs liquid operation on the sample tube, and then discards the pipette tip into the pipette tip recycling box 7.

[0098] (5) The gripper delivers the sample tube plate 18 to the low-temperature oscillation unit 10. The low-temperature oscillation unit 10 oscillates the sample tube according to the set time and temperature. After completion, the gripper outputs the sample tube plate 18.

[0099] (6) The gripper delivers the sample tube plate 18 to the low-speed centrifugation unit 12, and the low-speed centrifugation unit 12 throws off the vapor and liquid in the sample tube.

[0100] (7) The liquid injection unit transfers liquid from the sample tube to the well plate of the well plate holder 8;

[0101] (8) The gripper delivers the sample tube plate 18 to the magnetic rack unit 14, so that the magnetic beads adsorbing nucleic acid in the sample tube are attached to the tube wall or the bottom of the tube, and then the gripper outputs the sample tube rack.

[0102] (9) The clamps take the sample tube and place it in the PCR unit for amplification, and then send it to the fluorescence detection unit 16 for quantification.

[0103] Also included is the processing of the time axis, including standing, heat preservation and cooling, etc. Each flow can be a branch flow of the control unit, and the above-mentioned multiple flows or one flow and repeated operations can be combined with other flows to complete a complex flow to achieve sample nucleic acid detection processing. The above process can be achieved in the device as long as the branch flow of the complex flow is decomposed, and a new flow can be completed through the configuration of the control unit.

[0104] The above is only the preferred embodiment of the present application, and it should be pointed out that for ordinary skilled in the art, without departing from the principles of the present application, a number of improvements and refinements can be made, and these improvements and refinements should be considered as the protection scope of the present application.

Claims

1. A biological sample nucleic acid extraction processing system, characterized by: The system comprises a rack and a control unit, a work platform is arranged in the rack, a mechanical arm is arranged above the rack, a module unit is arranged on the work platform, comprising a sample tube plate rack, a suction head rack, a suction head recovery box, a hole plate rack, a low-temperature incubation unit, a low-temperature swing unit, a tube plate opening and closing cover unit, a low-speed centrifugal unit, a heating unit, a magnetic frame unit, a PCR unit and a fluorescence detection unit, the sample tube plate rack is arranged at the central position of the workbench, and other unit modules are arranged around the sample tube rack, a clamping jaw and a liquid injection unit are arranged on the mechanical arm, the sample tube plate rack is used for placing a sample tube plate, the tube plate clamping jaw is used for taking and placing the sample tube plate, and the sample tube is placed on the tube plate; the liquid injection unit completes liquid treatment in the sample tube through loading and unloading of the suction head; the suction head recovery box is used for collecting the unloaded suction head and waste liquid; the hole plate rack is used for liquid separation of the sample tube; the tube plate opening and closing cover unit is used for opening and closing the cover of the sample tube on the tube plate; the magnetic frame unit is used for adsorbing magnetic beads, and the magnetic beads are combined with nucleic acid substances; the PCR unit and the fluorescence detection unit realize amplification and quantification of the nucleic acid substances, the control unit controls the mechanical arm and each module unit to act, so that the nucleic acid extraction process is realized.

2. The biological sample nucleic acid extraction processing system according to claim 1, characterized by: The module unit further comprises a shock crushing unit and a high-speed centrifugal unit, which are arranged outside other module units, the shock crushing unit is used for crushing biological samples to obtain nucleic acid substances, and the high-speed centrifugal unit is used for centrifugal separation of the crushed samples.

3. The biological sample nucleic acid extraction processing system of claim 1, wherein: A sample tube plate is arranged on the sample tube plate rack, the sample tube plate is provided with four channels for placing four sample tubes, the sample tube is a centrifugal tube, and a positioning mounting hole is arranged at the end of the sample tube plate, which is commonly mounted on each module unit.

4. The biological sample nucleic acid extraction processing system according to any one of claims 1 to 3, characterized by: An array of clamping blocks in the front-rear direction is arranged on the work platform, clamping grooves are arranged on both sides of the base of each module unit, and each module unit is sequentially and multi-row clamped on the work platform through the clamping blocks and the clamping grooves.

5. The biological sample nucleic acid extraction processing system according to claim 4, characterized by: The sample tube plate rack, the suction head rack and the hole plate rack are multiple and arranged in one row or multiple rows, the suction head recovery box is arranged outside the suction head rack, and other module units are arranged in one row or multiple rows in the periphery.

6. The biological sample nucleic acid extraction processing system according to any one of claims 1 to 3, characterized by: Multiple sample tube plates are arranged on the sample tube plate rack, an array of suction heads is arranged on the suction head rack, the array of suction heads comprises multiple specifications of suction heads, a hole plate is arranged on the hole plate rack, the clamping jaw is used for taking and placing the sample tube plate and the hole plate, and the liquid injection unit is a four-channel pipettor.

7. The biological sample nucleic acid extraction processing system according to any one of claims 1 to 3, characterized in that: the low-temperature incubation unit comprises a low-temperature module and a container rack mounted on the low-temperature module, and the container rack corresponds to multiple specifications of containers; the low-temperature swing unit comprises a low-temperature module and a swing module, and the swing module drives the low-temperature module and a sample tube plate on the low-temperature module to swing and mix; the tube plate opening and closing cover unit comprises a tube plate seat and an opening and closing pressing plate, and the opening and closing of the sample tube on the tube plate seat is realized through the overturning action of the pressing plate; the low-speed centrifugal unit comprises a tube plate seat and a centrifugal motor, the tube plate seat is symmetrically arranged in two, and the centrifugal motor drives the tube plate seat to rotate slowly to realize centrifugation. The heating unit comprises a tube plate seat and an auxiliary heating table, a heating plate is arranged on the tube plate seat to heat the sample tube, and the auxiliary heating table is used to press and heat the heated pressure plate on the heating plate; The magnetic frame unit comprises a magnet base arranged in the frame, the magnet base is provided with a magnet, a tube plate is arranged on the frame, and the sample tube on the tube plate is matched with the magnet to realize the adsorption of the magnetic beads in the sample tube to the tube wall.

8. The biological sample nucleic acid extraction processing system according to claim 2, characterized by: The oscillation and crushing unit comprises a lifting seat and a driving motor, the driving motor drives the lifting seat to oscillate up and down through a crank wheel, the sample tube in the lifting seat is provided with a biological sample and a rigid particle, and the high-speed oscillation of the lifting seat makes the rigid particle crush the biological sample.

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