Automatic sampling system for culture tank

Abstract

The utility model relates to an automatic sampling system of a culture tank. Through accurate control of the control system, automation of the sampling process is achieved, manual intervention is not needed, the sampling efficiency is greatly improved, and the labor intensity is reduced. By utilizing the high-precision sensing device and the control system, the sampling time and the sampling amount can be accurately controlled, the accuracy and the consistency of each sampling are ensured, and the reliability of experimental data is improved. Due to the application of the sterile air driving mechanism and the one-way valve, external pollutants are effectively prevented from being introduced in the sampling process, the purity of a culture environment is guaranteed, and the pollution risk is reduced. By monitoring parameters such as pressure in the culture tank in real time and feeding data back to the control system, the system can intelligently regulate and control the sampling process, and smooth sampling operation is ensured. Through the automatic, high-precision and pollution-free design, the sampling efficiency and accuracy in the cell culture and fermentation process in the field of bioengineering are remarkably improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of bioengineering technology, more particularly to a kind of culture tank automatic sampling system. BACKGROUND

[0002] In the field of bioengineering, culture tank is the key component indispensable in cell culture and fermentation process, and is widely used in the culture and proliferation of animal cells, plant cells and microbial cells. These cell culture and fermentation processes are of great significance to the fields of biopharmaceuticals, agricultural biotechnology and industrial biotechnology. In order to ensure the smooth progress of these processes, it is necessary to accurately monitor various parameters in the fermentation process, such as cell density, metabolite concentration, pH value, temperature, etc., and the monitoring of these parameters usually relies on periodic sampling and analysis of the culture medium.

[0003] The traditional sampling method usually relies on manual operation, which not only is inefficient, increases the labor intensity of the operator, but also easily introduces pollution in the sampling process, damages the culture environment, and thus affects the growth and metabolism of cells, and may even lead to the failure of the entire fermentation process. In addition, manual sampling also has the problems of uneven sampling volume and inaccurate sampling time point, which greatly reduces the reliability and accuracy of the monitoring results.

[0004] In order to solve the above problems, it is particularly urgent and important to develop a system and method for automatically, accurately and non-polluting sampling from culture tank. SUMMARY

[0005] In view of the above technical problems, the utility model provides a kind of culture tank automatic sampling system, to realize the automation operation of sampling process by advanced control system and precision actuator, without manual intervention, reduce labor intensity, improve sampling efficiency. At the same time, through the design of high-precision sensing device and algorithm, sterile air driving mechanism and check valve, the sampling time, sampling volume and sampling position are accurately controlled, the accuracy and consistency of each sampling are ensured, and the sampling process is not contacted with the external environment, to avoid the introduction of pollutants, and ensure the purity of culture environment.

[0006] To achieve the above purpose, in the first aspect, the utility model provides a kind of culture tank automatic sampling system, comprising:

[0007] Sampling device, the sampling device includes culture tank, sampling tube, tee joint, fluid delivery control component, sample delivery tube and sample storage tank connected in turn, the culture tank is composed of tank body and cover body, the cover body is provided with the first through hole matched with the sampling tube;

[0008] The sterile air driving mechanism comprises a sterile air source, a first one-way valve and a gas conveying pipe, the sterile air source is connected to the tee joint through the gas conveying pipe, and the first one-way valve is arranged on the gas conveying pipe and used for controlling the flow of sterile air from the sterile air source to the culture tank.

[0009] One end of the sampling pipe extends into the through hole and reaches a preset position in the culture tank, and the one end of the sampling pipe is provided with a second one-way valve which only allows liquid to flow from the culture tank to the outside, and the other end of the sampling pipe is connected to the sample conveying pipe and the gas conveying pipe through the tee joint.

[0010] A first pressure sensing device is arranged on the sampling pipe.

[0011] A control system is electrically connected or signal connected with the first one-way valve, the second one-way valve, the fluid conveying control component and the first pressure sensing device.

[0012] Compared with the prior art, the above technical scheme realizes the automation of the sampling process through the accurate control of the control system, does not need manual intervention, greatly improves the sampling efficiency and reduces the labor intensity. By using the high-precision sensing device and the control system, the sampling time and the sampling amount can be accurately controlled, the accuracy and consistency of each sampling are ensured, and the reliability of experimental data is improved. The application of the sterile air driving mechanism and the one-way valve effectively prevents the introduction of external pollutants during the sampling process, ensures the purity of the culture environment and reduces the pollution risk. By monitoring the pressure and other parameters in the culture tank in real time and feeding the data back to the control system, the system can intelligently control the sampling process to ensure the smooth operation of the sampling operation. Equipped with perfect safety protection measures (such as overpressure protection), the sampling operation can be automatically stopped in abnormal conditions to ensure the safety and reliability of the equipment. The system can be widely applied to the sampling work of various culture tanks such as animal cells, plant cells and microbial cells, and has strong adaptability and flexibility. In summary, the culture tank automatic sampling system provided by the utility model improves the sampling efficiency and accuracy of the cell culture and fermentation process in the field of biological engineering through automation, high precision and pollution-free design, and provides strong support for the research and development in the related field.

[0013] In some embodiments, the sampling device further comprises a lifting mechanism connected to the control system, and the sampling pipe is arranged on the lifting mechanism. The lifting mechanism can be a common lifting mechanism such as an electric guide rail lifting mechanism, a pneumatic lifting mechanism, a servo motor and a screw transmission lifting mechanism, a stepping motor and a linear module lifting mechanism or a magnetic coupling driving lifting mechanism. The control of the lifting mechanism by the control system reaches the accurate sampling position, which can ensure the accuracy and consistency of sampling and improve the reliability of experimental data.

[0014] In some embodiments, the sterile air driving mechanism further comprises a sterile filter arranged between the sterile air source and the first one-way valve. During the sampling process, even if a sterile air source is used, there may still be tiny contaminated particles or microorganisms. Therefore, by arranging a sterile filter between the sterile air source and the first one-way valve, microorganisms and bacteria in the air can be efficiently removed, and these potential contaminants can be further removed, so that the gas entering the culture tank through the gas delivery pipe is completely sterile, thereby ensuring absolute sterility during the sampling process. Such design eliminates the influence of external contamination on experimental results, improves the accuracy and reliability of the experiment, protects cell culture from contamination, can reduce the corrosion and damage of bacteria, microorganisms and other substances to the internal components of the equipment, thereby prolonging the service life of the equipment, and reducing the number of experimental failures and re-experiments due to contamination, thereby improving production efficiency.

[0015] In some preferred embodiments, a second pressure sensing device is further included, arranged in the sample delivery pipe and electrically or signal connected with the control system. Signal connection refers to a signal connection mode realized by using wireless signal transmission technologies including but not limited to WiFi technology, Bluetooth, ZigBee, RFID (Radio Frequency Identification), NFC (Near Field Communication), sky wave propagation, microwave transmission and wireless SmartAir transmission, etc. During the sampling process, the pressure in the sample delivery pipe is an important parameter that directly affects the efficiency and stability of sample delivery. By monitoring the pressure in the sample delivery pipe in real time, the control system can adjust the working state of the fluid delivery control component according to the pressure change, thereby optimizing the sampling process and improving the sampling efficiency, and can ensure that the sample can be smoothly and quickly delivered to the sample storage tank. When the pressure in the sample delivery pipe is kept within a suitable range, it can also ensure that the sample will not be disturbed or lost during the delivery process, thereby improving the accuracy of the sampling. At the same time, such design can improve system safety, as excessive high or low pressure in the sample delivery pipe can cause system failure or safety accidents, therefore, by arranging a second pressure sensing device, potential safety hazards can be discovered and solved in time.

[0016] In some specific embodiments, the fluid delivery control component is an automatic valve. In this way, the automatic sampling demand can be met, and the automation control of the sampling process can be realized in the culture tank automatic sampling system, the human operation interference and error can be reduced, and the sampling efficiency can be improved. The automatic valve can accurately control the flow of fluid according to the preset program or the instruction of the control system, thereby meeting the demand of automatic control. The automatic valve has precise flow control ability, can adjust the flow of fluid as needed, and ensures the accuracy and consistency of sampling during the sampling process. The automatic valve can adapt to complex working environments such as different fluids, different pressures and different temperatures, and has strong adaptability and reliability.

[0017] In some specific embodiments, the fluid delivery control component is a peristaltic pump. The peristaltic pump is chosen for its working principle that the fluid only comes into contact with the pump tube, not directly with the pump body, which greatly reduces the possibility of contamination. For fluids that require high cleanliness or sterile processing, it provides higher safety and reliability. Peristaltic pumps have excellent repeatability and stability, and by adjusting the speed and frequency of the pump, precise flow control can be achieved to meet the requirements of flow accuracy. Moreover, the shear force generated by the peristaltic pump is very low, making it an ideal tool for delivering fluids that are sensitive to shear and aggressive, such as cells, proteins and other biologically active substances. Further, peristaltic pumps have good self-suction ability, can prevent backflow, and can run empty and dry, ensuring that the peristaltic pump operates efficiently and stably in various complex delivery environments.

[0018] In some embodiments, a third pressure sensing device is also included, which is arranged in the sterile air source and electrically or signal connected with the control system. Such design can ensure the stability and reliability of air supply by monitoring the pressure of sterile air source in real time. Pressure fluctuations or abnormalities in the sterile air source can pose potential risks to the system or the surrounding environment, and the third pressure sensing device can detect these abnormalities in a timely manner and take appropriate measures such as stopping delivery, alarming or automatically adjusting the pressure through the control system, thereby ensuring the safe operation of the system. Through the connection with the control system, the third pressure sensing device can realize automatic and intelligent pressure control. This not only improves production efficiency, but also reduces errors and uncertainties caused by human operation. Based on the data provided by the third pressure sensing device, the control system can optimize the operation of the entire system.

[0019] In some specific embodiments, the culture tank is provided with a temperature monitoring device, which is electrically or signal connected with the control system to monitor and control the temperature in the culture tank in real time. Temperature has an important influence on the growth and metabolism of microorganisms, and by monitoring and controlling the temperature in the culture tank in real time, it can ensure that the microorganisms are within the optimal growth range, thereby promoting their growth, metabolism and product production. Through the precise control of the control system on the temperature, the experimental or production process can be optimized, the accuracy of the experimental results can be improved, and the production efficiency can be improved, which helps to reduce the experimental failure or production delay caused by temperature fluctuations.

[0020] In a preferred embodiment, the control system comprises a pressure data processing unit, a temperature data processing unit, a sampling control unit, a sterile air control unit, and an alarm unit. The control system issues instructions to the first one-way valve, the second one-way valve, and / or the fluid delivery control component to sample, blow, or close, or issues an alarm for failure, based on the pressure and / or temperature data obtained by the pressure data processing unit and / or the temperature data processing unit. The pressure data processing unit and the temperature data processing unit can monitor the pressure and temperature in the bioreactor or other biological reaction equipment in real time, ensuring that they remain within the desired operating range, helping to prevent potential operational risks, and ensuring the accuracy and reliability of the experiment. Through the sampling control unit and the sterile air control unit, the control system can accurately control the actions of the first one-way valve, the second one-way valve, and the fluid delivery control component based on real-time data, to achieve the purpose of precisely controlling sampling, blowing, or closing to maintain a sterile environment, prevent contamination, and ensure experimental results. The alarm unit can issue an alarm when abnormal data (such as pressure or temperature exceeding the preset range) is detected, promptly informing the operator, helping to prevent potential safety incidents, and reducing the risk of equipment damage and experimental failure. Human operation often has errors and uncertainties, while an automated control system can eliminate these potential problems. By presetting parameters and algorithms, the control system can ensure that each experiment is operated according to the same standard, thereby improving the reliability and consistency of experimental results. Modern control systems usually support remote monitoring and management functions. This means that the operator can connect to the control system through the network from any location, view experimental data in real time, monitor equipment status, and issue control instructions. This flexibility is very useful for scenarios that require remote collaboration or distributed experimental environments.

[0021] The above description is only a summary of the technical solutions of the present application. In order for those skilled in the art to more clearly understand the technical solutions of the present application, and to implement the content recorded in the specification and drawings, and in order for the above and other purposes, features and advantages of the present application to be more easily understood, the following will be described in conjunction with the specific embodiments of the present application and the accompanying drawings. BRIEF DESCRIPTION OF DRAWINGS

[0022] The accompanying drawings are only used to illustrate the principles, implementation methods, applications, characteristics and effects of the specific embodiments of the present application and other related content, and cannot be considered as limitations of the present application.

[0023] In the drawings:

[0024] Figure 1 The schematic diagram of the automatic sampling system of the bioreactor provided for Example 1 is shown in Figure 1.

[0025] Figure 2 The schematic diagram of the automatic sampling system of the bioreactor provided for Example 2 is shown in Figure 2.

[0026] Figure 3 The schematic diagram of the automatic sampling system of the culture tank provided for Example 3 is shown in Figure 3.

[0027] Figure 4 The schematic diagram of the automatic sampling system of the culture tank provided for Example 4 is shown in Figure 4.

[0028] The reference signs involved in the above-mentioned figures are explained as follows:

[0029] 1. Culture tank; 11. Sampling tube; 111. One end; 112. The other end; 12. Peristaltic pump; 13. Sample delivery tube; 14. Cover; 141. Through hole; 15. Automatic valve;

[0030] 2. Three-way joint; 22. Second one-way valve;

[0031] 3. Sample storage tank; 31. First pressure sensing device; 32. Second pressure sensing device; 33. Temperature monitoring device;

[0032] 4. Sterile air source; 41. Sterile filter; 42. First one-way valve; 43. Air delivery tube;

[0033] 5. Control system; 51. Pressure data processing unit; 52. Temperature data processing unit; 53. Sampling control unit; 54. Sterile air control unit; 55. Alarm unit. DETAILED DESCRIPTION

[0034] In order to describe possible application scenarios, technical principles, specific implementable schemes, and purposes and effects that can be achieved of the present application in detail, the following will be described in detail in combination with specific examples listed and with the aid of the accompanying drawings. The examples described in the present text are only used to more clearly illustrate the technical schemes of the present application, and therefore cannot be used to limit the protection scope of the present application.

[0035] In the present text, the term “example” means that the specific features, structures or characteristics described in combination with the examples can be included in at least one example of the present application. The term “example” appearing at various positions in the specification does not necessarily refer to the same example, and does not particularly limit the independence or association between other examples. In principle, in the present application, as long as there is no technical contradiction or conflict, the technical features mentioned in each example can be combined in any way to form a corresponding implementable technical scheme.

[0036] Unless otherwise defined, the meanings of the technical terms used in the present text are the same as those commonly understood by the person skilled in the art to which the present application belongs; the use of relevant terms in the present text is only for the purpose of describing specific examples, and is not intended to limit the present application.

[0037] In the description of the present application, the phrase "and / or" is a description of a logical relationship between objects, which means that there can be three relationships, for example, A and / or B, which means that there are three cases: A exists, B exists, and A and B exist at the same time. In addition, the character " / " herein generally represents that the associated objects before and after are an "or" logical relationship.

[0038] In the present application, the terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual quantity, primary and secondary, or order relationship between the entities or operations.

[0039] In the present application, without more limitation, the "includes", "contains", "has" or other similar expressions used in the sentence are intended to cover non-exclusive inclusion, and these expressions do not exclude the presence of other elements in the process, method or product including the described elements, so that the process, method or product including a series of elements can not only include those limited elements, but also include other elements not explicitly listed, or also include the elements inherent in such process, method or product.

[0040] As the same understanding as in the "Guidelines for Examination", in the present application, the expressions such as "greater than", "less than", "exceed" are understood as not including the number; the expressions such as "above", "below", "within" are understood as including the number. In addition, in the description of the embodiments of the present application, the meaning of "multiple" is more than two (including two), and similar expressions related to "multiple" are also understood in this way, for example, "multiple groups", "multiple times" and the like, unless otherwise explicitly limited.

[0041] In the description of the embodiments of the present application, the spatial-related expressions used, such as "center", "longitudinal", "transverse", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "vertical", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the specific embodiments or the drawings, and are only for the convenience of describing the specific embodiments of the present application or for the reader to understand, and do not indicate or imply that the indicated device or component must have a specific position, a specific orientation, or be constructed or operated in a specific orientation, and therefore cannot be understood as a limitation on the embodiments of the present application.

[0042] Unless otherwise clearly indicated or implied to the contrary by context, the word "comprise", "comprising", and "comprises" and the like are used inclusively and not exclusivity, i.e., in a non-limiting sense; "coupled" means directly or indirectly connected; "fixed" means affixed, connected, attached, or the like; "set" means fixed, connected, attached, or the like; "connected" means directly or indirectly connected; "indirectly connected" means via one or more other elements; "interact" means to affect or be affected by another element; and the like. The terms "comprise", "comprising", "comprises", "include", "including", and "includes" as well as "comprised of" and / or "comprising of" when used in this specification, specify the presence of stated features, integers, steps, operations, objects, and / or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, objects, components, and / or groups thereof.

[0043] Embodiment One

[0044] Automatic sampling system for culture tank

[0045] Referring to Figure 1 The automatic sampling system for culture tank according to the embodiment comprises the following components:

[0046] Sampling device: comprising culture tank 1, sampling tube 11, three-way joint 2, peristaltic pump 12, sample delivery tube 13 and sample storage tank 3. The culture tank 1 is provided with a cover 14, and the cover 14 is provided with a through hole 141 adapted to the sampling tube.

[0047] Sterile air driving mechanism: comprising sterile air source 4, sterile filter 41, first one-way valve 42 and air delivery tube 43. The sterile air source 4 is connected to the three-way joint 2 through the sterile filter 41, the first one-way valve 42 and the air delivery tube 43.

[0048] Sampling tube: one end 111 extends into the preset position of the culture tank 1 and is provided with a second one-way valve 22, and the other end 112 is connected to the sample delivery tube 13 and the air delivery tube 43 through the three-way joint 2.

[0049] First pressure sensing device 31: provided on the sampling tube.

[0050] Control system 5: electrically connected to the first one-way valve 42, the second one-way valve 22, the peristaltic pump 12 and the first pressure sensing device 31.

[0051] The operation process of the automatic sampling system for culture tank according to the embodiment is as follows:

[0052] The control system sends an instruction to open the first one-way valve, and the sterile air enters the culture tank through the air delivery tube to blow away the liquid at the sampling position to avoid blockage.

[0053] The first one-way valve is closed, the second one-way valve and the peristaltic pump are opened, and the liquid sample is sucked into the sample delivery tube by the peristaltic pump through the sampling tube and is delivered to the sample storage tank.

[0054] The control system adjusts the rotation speed of the peristaltic pump according to the pressure data of the first pressure sensing device to ensure stable sampling.

[0055] Embodiment Two

[0056] Automatic sampling system for culture tank

[0057] Referring to Figure 2 , the automatic sampling system for culture tank of the present embodiment adds the following components on the basis of Embodiment One:

[0058] Second pressure sensing device 32: provided in the sample delivery pipe.

[0059] Temperature monitoring device 33: provided in the culture tank and electrically connected to the control system.

[0060] Control system 5: including pressure data processing unit 51, temperature data processing unit 52, sampling control unit 53, sterile air control unit 54, and alarm unit 55.

[0061] The operation process of the automatic sampling system for culture tank of the present embodiment is as follows:

[0062] The temperature monitoring device monitors the temperature in the culture tank in real time and sends it to the sampling control unit through the temperature data processing unit.

[0063] When the temperature reaches the preset value, the sampling control unit issues an instruction to start the sampling process in Embodiment One.

[0064] The second pressure sensing device monitors the pressure in the sample delivery pipe in real time, and if the pressure is abnormal, the alarm unit issues an alarm.

[0065] Embodiment Three

[0066] Automatic sampling system for culture tank

[0067] Referring to Figure 3 , the automatic sampling system for culture tank of the present embodiment replaces the peristaltic pump with an automatic valve 15 on the basis of Embodiment Two.

[0068] Replace the fluid delivery control component of Embodiment Two with an automatic valve 15.

[0069] The operation process of the automatic sampling system for culture tank of the present embodiment is as follows:

[0070] The control system controls the opening and closing of the first one-way valve and the automatic valve through the sterile air control unit and the sampling control unit, realizes the blowing and sampling of the culture tank.

[0071] The second pressure sensing device and the temperature monitoring device continue to monitor the pressure and temperature in the sample delivery pipe and the culture tank, and make corresponding adjustments or issue alarms through the control system.

[0072] Embodiment Four

[0073] An automatic sampling system for culture tank

[0074] Please refer to Figure 4 The automatic sampling system for culture tank of the embodiment comprises a culture tank 1, a sampling tube 11, a three-way joint 2, a peristaltic pump 12, a sample delivery tube 13, and a sample storage tank 3.

[0075] A sterile air source 4, a sterile filter 41, a first one-way valve 42, and a gas delivery tube 43.

[0076] A lifting mechanism 16 connected to a control system 5, and the sampling tube 11 is arranged on the lifting mechanism 16.

[0077] A first pressure sensing device 31 arranged on the sampling tube 11.

[0078] The control system 5 is electrically connected to the first one-way valve 42, the peristaltic pump 12, the lifting mechanism 16, and the first pressure sensing device 31.

[0079] The operation process of the automatic sampling system for culture tank of the embodiment is as follows:

[0080] The control system adjusts the sampling position of the sampling tube in the culture tank through the lifting mechanism.

[0081] The control system opens the first one-way valve, and the sterile air enters the culture tank through the gas delivery tube to blow away the liquid at the sampling position.

[0082] The first one-way valve is closed, the peristaltic pump is started, the liquid sample is sucked through the sampling tube and delivered to the sample delivery tube, and finally enters the sample storage tank.

[0083] The control system adjusts the rotation speed of the peristaltic pump according to the data of the first pressure sensing device.

[0084] Finally, it needs to be pointed out that although the above-mentioned embodiments have been described in the specification and drawings of the present application, it does not limit the patent protection scope of the present application. Any equivalent structure or equivalent flow replacement or modification of the technical solutions based on the essential concept of the present application, using the content described in the specification and drawings, and directly or indirectly implementing the technical solutions of the above embodiments in other related technical fields, etc., are all included in the patent protection scope of the present application.

Claims

1. An automatic sampling system for a culture vessel, characterized in that, include: A sampling device, comprising a culture tank, a sampling tube, a three-way connector, a fluid transport control component, a sample delivery tube, and a sample storage tank connected in sequence, wherein the culture tank consists of a tank body and a cover body, and the cover body is provided with a first through hole adapted to the sampling tube; A sterile air drive mechanism includes a sterile air source, a first one-way valve, and an air supply pipe. The sterile air source is connected to the three-way connector through the air supply pipe. The first one-way valve is disposed on the air supply pipe and is used to control the flow of sterile air from the sterile air source to the culture tank. One end of the sampling tube extends into the through hole and enters the preset position of the culture tank, and a second one-way valve is provided at one end of the sampling tube. The second one-way valve only allows liquid to flow from the culture tank to the outside. The other end of the sampling tube is connected to the sample delivery tube and the gas delivery tube by a three-way connector. A first pressure sensing device is disposed in the sampling tube; and The control system is electrically or signal-connected to the first check valve, the second check valve, the fluid delivery control component, and the first pressure sensing device.

2. The automatic sampling system for culture vessels according to claim 1, characterized in that, The sampling device also includes a lifting mechanism connected to the control system, and the sampling tube is disposed on the lifting mechanism.

3. The automatic sampling system for culture vessels according to claim 1, characterized in that, The sterile air drive mechanism also includes a sterile filter disposed between the sterile air source and the first one-way valve.

4. The automatic sampling system for culture vessels according to claim 1, characterized in that, It also includes a second pressure sensing device, which is disposed in the sample delivery tube and is electrically or signal-connected to the control system.

5. The automatic sampling system for culture vessels according to claim 1, characterized in that, The fluid delivery control component is an automatic valve.

6. The automatic sampling system for culture vessels according to claim 1, characterized in that, The fluid delivery control component is a peristaltic pump.

7. The automatic sampling system for culture vessels according to claim 1, characterized in that, It also includes a third pressure sensing device, which is located at the sterile air source and is electrically or signal-connected to the control system.

8. The automatic sampling system for culture vessels according to claim 1, characterized in that, The culture tank is equipped with a temperature monitoring device, which is electrically or signal-connected to the control system to monitor and control the temperature inside the culture tank in real time.

9. The automatic sampling system for culture vessels according to any one of claims 1-8, characterized in that, The control system includes a pressure data processing unit, a temperature data processing unit, a sampling control unit, a sterile air control unit, and an alarm unit. Based on the pressure and / or temperature data obtained by the pressure data processing unit and / or the temperature data processing unit, the system issues sampling, air blowing, or closing commands to the first check valve, the second check valve, and / or the fluid delivery control component, or issues a fault alarm.

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