Sample application device and sample application equipment

Abstract

The embodiment of the utility model provides a sample application device and sample application equipment. The sample application device comprises a sample application assembly, the sample application assembly comprises a sample application needle and a cover pressing part, the cover pressing part comprises a cover opening part and a cover pressing mechanism arranged on one side of the cover opening part, a penetrating hole for the sample application needle to penetrate through is formed in the cover opening part, and the sample application assembly is arranged in the mode that in the liquid suction process, the sample application needle and the cover pressing part descend, and the sample application needle is inserted into the penetrating hole; the cap pressing mechanism is used for pressing a bottle cap before the cap opening part opens the bottle cap of the sample application bottle, so that the sample application needle penetrates through the bottle cap and extends into the sample application bottle, and the cap pressing mechanism is separated from the bottle cap later than the cap opening part after the sample application needle completing liquid suction is pulled out of the sample application bottle. In the process that the cap opening part is separated from the bottle cap of the sample application bottle, the cap pressing mechanism can always press the bottle cap of the sample application bottle, so that the sample application bottle can be kept still. Therefore, even if friction is generated between the cap opening part and the bottle cap, the cap opening part cannot move along with the sample application bottle. Therefore, the sample application needle can smoothly carry out subsequent sample application operation.

Description

TECHNICAL FIELD

[0001] The utility model relates to spot sample technology field, specifically, relate to a spot sample device and have its spot sample equipment. BACKGROUND

[0002] Thin layer chromatography spot sample experiment has extensive application in chemical, biological, pharmaceutical, environmental etc. fields. Taking chemical field as an example, thin layer chromatography spot sample experiment can be used for separating and identifying compounds: by smearing the mixture to be separated on thin layer chromatography plate, then carrying out experimental analysis, the relative migration rate and relative content of each component can be obtained, thereby realizing the separation and identification of mixture; in addition, thin layer chromatography spot sample experiment can also be used for analyzing the progress and product of reaction. By sampling in the reaction process, then analyzing on thin layer chromatography plate, the progress of reaction and the generation of product can be understood.

[0003] Thin layer chromatography spot sample experiment needs to be absorbed by spot sample device. Specifically, spot sample bottle can contain spot sample liquid. The uncapping part of spot sample device can open the bottle cap, and then the spot sample needle can pass through the bottle cap of spot sample bottle, so as to extend into the spot sample bottle, and then the spot sample liquid in the spot sample bottle can be sucked. When the absorption is completed, the spot sample needle can be pulled out of the spot sample bottle, so as to be separated from the spot sample bottle. In this way, the spot sample needle can carry out subsequent spot sample operation.

[0004] However, in the process of uncapping part opening bottle cap and separating from bottle cap, uncapping part will rub with bottle cap, so as to cause uncapping part to move together with spot sample bottle. In this way, under the influence of spot sample bottle, spot sample needle cannot execute subsequent spot sample operation, so the reliability of spot sample device is poor. Moreover, spot sample bottle can be damaged by colliding with other components, so as to cause dangerous accident. UTILITY MODEL CONTENTS

[0005] In order to at least partially solve the problems existing in the prior art, according to one aspect of the utility model, a kind of spot sample device is provided. Spot sample device includes: spot sample component, spot sample component includes spot sample needle and gland piece, gland piece includes uncapping part and the gland mechanism being arranged at one side of uncapping part, uncapping part is provided with the hole that spot sample needle passes through, and spot sample component is arranged as follows: in the process of absorption, spot sample needle and gland piece descend, and gland mechanism is pressed bottle cap before uncapping part opens the bottle cap of spot sample bottle, so that spot sample needle passes through bottle cap and extends into spot sample bottle, and after the spot sample needle that completes absorption is pulled out from spot sample bottle, gland mechanism is separated from bottle cap later than uncapping part.

[0006] Exemplarily, uncapping part includes expansion boss for expanding the sealing layer on bottle cap, to separate sealing layer and spot sample needle.

[0007] Exemplarily, the capping mechanism comprises a pressing rod and an elastic member, the upper end of the elastic member is connected to one side of the capping part of the capping member and the lower end is connected to the pressing rod, the pressing rod is used to press and release the bottle cap, the pressing rod presses the bottle cap before the capping part opens the bottle cap, so as to compress the elastic member and make the elastic member have the elastic potential energy to drive the pressing rod to protrude downward from the capping part.

[0008] Exemplarily, the pressing rod comprises a pressing part and an extension rod extending upward from the pressing part, the elastic member is sleeved on the extension rod and the lower end is connected to the pressing part.

[0009] Exemplarily, a pressing rod through hole is arranged on one side of the capping part of the capping member, and the extension rod passes through the pressing rod through hole.

[0010] Exemplarily, a positioning boss is protruded downward from the outer edge of the capping member, and the positioning boss is used to sleeve the top of the bottle cap before the capping mechanism presses the bottle cap.

[0011] Exemplarily, the spotting device further comprises a driving assembly connected to the spotting needle and the capping member, the driving assembly is used to drive the spotting needle and the capping member to ascend and descend, so as to make the capping mechanism press and release the bottle cap, the capping part opens the bottle cap, the spotting needle extends into the spotting bottle through the through hole, absorbs the spotting liquid in the spotting bottle, and the spotting needle releases the spotting bottle through the through hole.

[0012] Exemplarily, the spotting device further comprises a first horizontal driving member and a second horizontal driving member, the driving end of the first horizontal driving member is connected to the main body of the driving assembly, so as to drive the driving assembly to move along a first horizontal direction, and the driving end of the second horizontal driving member is connected to the main body of the first horizontal driving member, so as to drive the first horizontal driving member and the driving assembly to move along a second horizontal direction perpendicular to the first horizontal direction.

[0013] Exemplarily, the spotting needle comprises a needle tube, a needle head and a piston rod, the piston rod is liftable in the needle tube, and the needle head is integrally connected to the lower end of the needle tube.

[0014] Exemplarily, the outer diameter of the needle head is between 0.4mm and 0.6mm.

[0015] Exemplarily, the inner diameter of the needle head is between 0.1mm and 0.3mm.

[0016] Exemplarily, the spotting device further comprises a spotting table and a positioning assembly, the spotting table is provided with a groove for placing a spotting plate, the positioning assembly is used to position the spotting plate in the groove, and the spotting needle is used to spot on the spotting plate.

[0017] For example, the spotting apparatus further includes a detector and a controller electrically connected to the detector. The detector is located to the side of the spotting plate and is used to detect the height of the non-transparent top surface of the spotting plate at an angle downwards to generate and send a signal to the controller. The controller is used to ensure that the spotting needle has a desired height difference with the non-transparent top surface when the spotting needle is spotting, based on the signal.

[0018] For example, the sampling device further includes a reference calibration block, the lower end of which is used to contact the reference calibration block so that the position of the sampling needle is used as a reference position.

[0019] According to another aspect of this utility model, a sampling device is also provided. The sampling device includes any of the sampling apparatuses described above.

[0020] The sampling device provided in this embodiment features a capping mechanism. During the process of the capping part detaching from the cap of the sampling bottle, the capping mechanism maintains pressure on the cap, keeping the sampling bottle stationary. Thus, even if friction occurs between the capping part and the cap, the capping part cannot move the sampling bottle along with it. Therefore, the sampling needle can smoothly perform subsequent sampling operations, resulting in high reliability and a better user experience. Furthermore, during the insertion of the sampling needle into the sampling bottle and the aspiration of the sampling liquid, the capping mechanism also keeps the sampling bottle fixed, ensuring that the sampling needle can perform the operation without interference.

[0021] This utility model description introduces a series of simplified concepts, which will be further explained in detail in the detailed description section. This utility model description is not intended to limit the key features and essential technical features of the claimed technical solution, nor is it intended to determine the scope of protection of the claimed technical solution.

[0022] The advantages and features of this utility model will be described in detail below with reference to the accompanying drawings. Attached Figure Description

[0023] The following drawings, which are incorporated herein by reference as part of this invention, are provided for understanding the invention. The drawings illustrate embodiments of the invention and their descriptions, serving to explain the principles of the invention. In the drawings,

[0024] Figure 1 This is a perspective view of a sampling device according to an exemplary embodiment of the present invention;

[0025] Figure 2 for Figure 1 A perspective view of part of the structure of the spotting device shown in the figure;

[0026] Figure 3 for Figure 2 The image shows a front view of a portion of the structure of the spotting device;

[0027] Figure 4 For Figure 3 a sectional view of a partial structure of a dispensing device shown in

[0028] Figure 5 For Figure 2 a perspective view of a partial structure of a cover shown in

[0029] Wherein, the above figures include the following reference signs:

[0030] 100, a dispensing frame; 110, a first horizontal driving member; 120, a second horizontal driving member; 200, a driving assembly; 210, a first driving member; 220, a second driving member; 230, a third driving member; 240, a first connecting frame; 250, a second connecting frame; 260, a third connecting frame; 270, a first sliding rail; 280, a second sliding rail; 291, a first screw rod; 292, a first nut; 293, a second screw rod; 294, a second nut; 300, a dispensing assembly; 310, a dispensing needle; 311, a needle tube; 312, a needle head; 313, a piston rod; 320, a cover; 321, a through hole; 322, an uncovering part; 323, a positioning boss; 325, a pressing rod through hole; 331, a needle base; 332, a dispensing needle cover plate; 400, a cover mechanism; 410, a pressing rod; 411, a pressing part; 412, an extension rod; 420, an elastic member. DETAILED DESCRIPTION

[0031] In the following description, numerous specific details are provided in order to provide a thorough understanding of the present application. One of ordinary skill in the art will realize, however, that the application can be practiced without one or more of these details. In other instances, well-known features have not been described in detail in order not to unnecessarily obscure the present application.

[0032] According to an aspect of the present application, a dispensing device is provided. The dispensing device can be used to draw dispensing liquid in a dispensing bottle, and then subsequent operations such as dispensing can be performed. The dispensing device can be applied to any suitable equipment, including but not limited to a dispensing equipment. Therefore, according to another aspect of the present application, a dispensing equipment is also provided. The dispensing device and the dispensing equipment of the embodiments of the present application will be described in detail below with reference to the accompanying drawings.

[0033] As Figures 1-5 shown, the dispensing device can include a dispensing assembly 300. The dispensing assembly 300 can include a dispensing needle 310 and a cover 320.

[0034] The spotting needle 310 can be of various types known in the art or to be developed in the future, such as a high-precision micro spotting needle.

[0035] The capping member 320 can include a capping portion 322 and a capping mechanism 400. The capping portion 322 can be provided with a through hole 321. The through hole 321 can be for the spotting needle 310 to pass through. The spotting needle 310 can be raised and lowered along the through hole 321. The capping mechanism 400 can be provided on one side of the capping portion 322. The spotting assembly 300 can be configured such that during the process of liquid absorption, the spotting needle 310 and the capping member 320 can be lowered, the capping mechanism 400 can press the cap of the spotting bottle before the capping portion 322 opens the cap of the spotting bottle, so that the spotting needle 310 can pass through the cap of the spotting bottle and extend into the spotting bottle, and after the spotting needle 310 completes the liquid absorption and is withdrawn from the spotting bottle, the capping mechanism 400 can be separated from the cap later than the capping portion 322. The spotting needle 310 and the capping member 320 can be raised and lowered by manual means or a motor or the like driving member.

[0036] In actual application, the spotting assembly 300 can be located above the spotting bottle. Then, the spotting needle 310 and the capping member 320 can be lowered, so as to approach the spotting bottle. Then, the capping mechanism 400 can press the cap of the spotting bottle, so as to fix the spotting bottle. Then, the capping portion 322 can open the cap of the spotting bottle. Depending on the structure of the cap, the capping portion 322 can have different structures, such as a knife or the like, as long as it can open the cap of the spotting bottle. Then, the spotting needle 310 can pass through the through hole 321 and the cap of the spotting bottle, and extend into the spotting bottle, to absorb the spotting liquid in the spotting bottle. After the spotting needle 310 completes the liquid absorption, the spotting needle 310 and the capping member 320 can be raised, the spotting needle 310 can pass through the through hole 321 and be withdrawn from the spotting bottle, to be separated from the spotting bottle. Then, the capping portion 322 can be separated from the cap of the spotting bottle. Then, the capping mechanism 400 can be separated from the cap of the spotting bottle. The structure of the capping mechanism 400 can be arbitrary, as long as it can press and fix the cap of the spotting bottle. When spotting, the spotting assembly 300 can be located above the spotting plate. The spotting needle 310 can be raised, lowered or kept stationary, so as to have a desired height difference between the spotting needle 310 and the spotting plate. Then, the spotting needle 310 can spot the spotting liquid absorbed thereby to the spotting plate.

[0037] In conclusion, the sample spotting device provided by the embodiment of the present application can keep the sample spotting bottle from moving because the pressing cover mechanism 400 can press the bottle cap of the sample spotting bottle during the process that the cap opening part 322 is separated from the bottle cap of the sample spotting bottle, so that the sample spotting bottle can be kept from moving even if the cap opening part 322 rubs against the bottle cap. Therefore, the sample spotting needle 310 can smoothly perform the subsequent sample spotting operation, the reliability of the sample spotting device is high, and the user experience is good. In addition, during the process that the sample spotting needle 310 is inserted into the sample spotting bottle and the sample spotting liquid in the sample spotting bottle is sucked, the sample spotting bottle can also be kept from moving under the pressing of the pressing cover mechanism 400, so that the sample spotting needle 310 can perform the operation without being affected.

[0038] For some sample spotting bottles, a through hole is usually arranged on the bottle cap. The through hole can be covered with a sealing layer. Because the sealing layer is arranged, the bottle cap can be easily opened. The structure of the sealing layer can be arbitrary. In some embodiments, the sealing layer can be made of elastic materials such as silica gel. A slit in the shape of a horizontal line or a cross can be arranged on the sealing layer. Generally, the sealing layer can close the slit by relying on its own elasticity.

[0039] As shown in Figs. 1 and 2, Figure 2 and 5 the cap opening part 322 can include an expanding boss. The through hole 321 can pass through the expanding boss. The expanding boss can be used to expand the sealing layer on the bottle cap to separate the sealing layer from the sample spotting needle 310. Because the sample spotting needle 310 passes through the through hole 321 and is not in contact with the sealing layer under the action of the expanding boss, the structure of the sealing layer will not be damaged due to the expansion of the sealing layer. Especially for the sealing layer with the gap as described above, if the expanding boss is not arranged, when the sample spotting needle 310 expands the slit, the sealing layer will always abut against the sample spotting needle 310 due to the elasticity. In this way, during the process that the sample spotting needle 310 is lifted to be separated from the sample spotting bottle, the sealing layer will scrape off the sample spotting liquid on the sample spotting needle 310, so that the amount of the sample spotting liquid that has been sucked is reduced, and the sample spotting effect is further reduced. By arranging the expanding boss, the sealing layer will not be in contact with the sample spotting needle 310, and the sample spotting needle 310 can suck the expected amount of sample spotting liquid.

[0040] As shown in Figs. 1 and 2, Figure 5 the expanding boss can be tapered downward. In this way, the area of the lower end of the expanding boss is small, so that the pressure acting on the sealing layer can be increased, and the expansion of the sealing layer can be facilitated. In addition, because the side surface of the expanding boss is an inclined surface, the resistance when the expanding boss is separated from the sealing layer is small.

[0041] As shown in Figs. 1 and 2, Figures 2-4As shown, the spotting needle 310 can include a needle tube 311, a needle head 312 and a piston rod 313. The needle head 312 can be connected to the lower end of the needle tube 311. The piston rod 313 is movably inserted into the upper end of the needle tube 311. The piston rod 313 can be lifted and lowered within the needle tube 311, so that the spotting needle 310 can suck and spot. Specifically, the needle head 312 can be lowered along the through hole 321, so that it can extend into the spotting bottle. Then, the piston rod 313 can be lifted, so that the spotting liquid in the spotting bottle can be sucked through the needle head 312. After the spotting needle 310 completes the sucking, the needle head 312 can be lifted along the through hole 321, so that it can pass through the through hole 321 and be separated from the spotting bottle. When spotting, the piston rod 313 can be lowered, so that the spotting liquid can be spotted onto the spotting plate through the needle head 312. The needle head 312 can be integrally connected to the lower end of the needle tube 311 by any suitable means such as welding, bonding or injection molding. In this way, the connection strength of the needle head 312 and the needle tube 311 is high, especially for the needle head 312 of the micro-spotting needle, which has a small diameter, so it is particularly important to improve the connection strength with the needle tube 311. Moreover, compared with the detachable connection of the needle tube and the needle head, the present solution can avoid the deviation of the position of the needle head 312 caused by installation errors, thereby affecting the sucking and spotting.

[0042] Exemplarily, the outer diameter of the needle head 312 can be between 0.4mm and 0.6mm. The outer diameter of the needle head 312 can be, for example, 0.4mm, 0.5mm or 0.6mm. If the outer diameter of the needle head 312 is too large, it will occupy a large space and have poor applicability. If the outer diameter of the needle head 312 is too small, the structural strength will be low, so that bending, breaking and other problems are likely to occur.

[0043] Exemplarily, the inner diameter of the needle head 312 can be between 0.1mm and 0.3mm. The inner diameter of the needle head 312 can be, for example, 0.1mm, 0.2mm or 0.3mm. If the inner diameter of the needle head 312 is too large, the structural strength will be low, so that bending, breaking and other problems are likely to occur. If the inner diameter of the needle head 312 is too small, the resistance of sucking and spotting will be too large.

[0044] Exemplarily, the spotting needle 310 can be a corrosion-resistant needle. In this way, the spotting needle 310 can suck and spot the spotting liquid with corrosive properties, and the applicability of the spotting needle 310 is high.

[0045] Exemplarily, the spotting needle 310 can include a plurality, including but not limited to two as shown in the figure, for example, three or more. Each of the plurality of spotting needles 310 can correspondingly suck the spotting liquid in the spotting bottle and correspondingly spot onto the spotting plate. In this way, the working efficiency of the spotting assembly 300 for sucking and spotting is high. The plurality of spotting needles 310 can be arranged in the horizontal direction.

[0046] For example, such as Figures 2-4 As shown, the sampling device may further include a drive assembly 200. The drive end of the drive assembly 200 may be connected to the sampling needle 310 and the capping member 320. The drive assembly 200 may be used to drive the sampling needle 310 and the capping member 320 to move up and down, so that the capping mechanism 400 presses and releases the cap, the capping part 322 opens and releases the cap, the sampling needle 310 passes through the through hole 321 and extends into the sampling bottle to draw the sampling liquid in the sampling bottle, and the sampling needle 310 passes through the through hole 321 and exits the sampling bottle.

[0047] Specifically, the drive assembly 200 can drive the sampling needle 310 and the capping member 320 to descend, so that the capping mechanism 400 can press the cap of the sampling bottle. Then, the capping part 322 can open the cap of the sampling bottle. Then, the driving sampling needle 310 can pass through the outlet hole 321 and extend into the sampling bottle. Then, the drive assembly 200 can drive the piston rod 313 of the sampling needle 310 to rise, so as to draw up the sampling liquid in the sampling bottle. After the sampling needle 310 completes the liquid drawing, the drive assembly 200 can drive the sampling needle 310 and the capping member 320 to rise, and the sampling needle 310 can pass through the outlet hole 321 and be withdrawn from the sampling bottle to detach from the sampling bottle. Then, the capping part 322 and the capping mechanism 400 can sequentially detach from the cap of the sampling bottle. The drive assembly 200 includes, but is not limited to, a motor drive assembly, a cylinder drive assembly, and / or an electric cylinder drive assembly. By setting the drive component 200, the sampling device has a high degree of automation, which can reduce labor costs.

[0048] For example, such as Figure 1As shown, the sampling device may further include a first horizontal drive 110 and a second horizontal drive 120. The drive end of the first horizontal drive 110 can be connected to the main body of the drive assembly 200. Under the drive of the first horizontal drive 110, the drive assembly 200 and the sampling assembly 300 can move along a first horizontal direction. The drive end of the second horizontal drive 120 can be connected to the main body of the first horizontal drive 110. Under the drive of the second horizontal drive 120, the first horizontal drive 110, the drive assembly 200, and the sampling assembly 300 can move along a second horizontal direction. The second horizontal direction may be perpendicular to the first horizontal direction. The first horizontal drive 110 includes, but is not limited to, a motor, a cylinder, or an electric cylinder. The second horizontal drive 120 includes, but is not limited to, a motor, a cylinder, or an electric cylinder. With the cooperation of the first horizontal drive 110 and the second horizontal drive 120, the drive assembly 200 and the sampling assembly 300 can move within the first and second horizontal directions, thereby moving to any suitable position along the horizontal direction. For example, the first horizontal drive 110 and the second horizontal drive 120 can drive the sampling assembly 300 to move above the sampling bottle so that the sampling needle 310 can aspirate liquid. After aspiration is complete, the first horizontal drive 110 and the second horizontal drive 120 can drive the sampling assembly 300 to move above the sampling plate so that the sampling needle 310 can spot the sample onto the sampling plate. Compared to using a robotic arm or other drive components, the first horizontal drive 110 and the second horizontal drive 120 can precisely control the position of the sampling assembly 300, thereby improving the accuracy of liquid aspiration and spotting by the sampling needle 310.

[0049] For example, such as Figure 1 As shown, the dispensing device may further include a dispensing frame 100. The dispensing frame 100 can be fixed to a device such as the ground or a table, thus serving as the base of the dispensing device. The structure of the dispensing frame 100 can be arbitrary. The main body of the driving assembly 200 can be disposed on the dispensing frame 100. In an embodiment where the dispensing device includes a first horizontal driving member 110 and a second horizontal driving member 120, the main body of the second horizontal driving member 120 can be disposed on the dispensing frame 100.

[0050] For example, such as Figures 2-4As shown, the driving assembly 200 can include a first driving member 210, a second driving member 220, and a third driving member 230. The driving end of the first driving member 210 can be connected to the cover member 320 for driving the cover member 320 to lift and lower. The driving end of the second driving member 220 can be connected to the main body of the spotting needle 310 (including the needle tube 311 and / or the needle head 312) for driving the spotting needle 310 to lift and lower as a whole, so as to extend into and out of the spotting bottle through the through hole 321. The driving end of the third driving member 230 can be connected to the piston rod 313 of the spotting needle 310 for driving the piston rod 313 to lift and lower, so as to suck and spot the spotting liquid. The first driving member 210 includes but is not limited to a motor, a pneumatic cylinder, or an electric cylinder. The second driving member 220 includes but is not limited to a motor, a pneumatic cylinder, or an electric cylinder. The third driving member 230 includes but is not limited to a motor, a pneumatic cylinder, or an electric cylinder. Specifically, the first driving member 210 can drive the cover member 320 to lower, so that the cover mechanism 400 presses the bottle cap of the spotting bottle, and then the cap opening part 322 opens the bottle cap of the spotting bottle. Then, the second driving member 220 can drive the spotting needle 310 to lower, so that the spotting needle 310 can extend into the spotting bottle through the through hole 321. Then, the third driving member 230 can drive the piston rod 313 of the spotting needle 310 to rise, so as to suck the spotting liquid in the spotting bottle. When the spotting needle 310 finishes sucking the liquid, the second driving member 220 can drive the spotting needle 310 to rise, so as to out of the spotting bottle through the through hole 321. Then, the first driving member 210 can drive the cover member 320 to rise, so that the cap opening part 322 and the cover mechanism 400 can out of the bottle cap of the spotting bottle in turn. When spotting is needed, the first driving member 210 and / or the second driving member 220 can drive the spotting needle 310 to have a desired height difference with the spotting plate. Then, the third driving member 230 can drive the piston rod 313 of the spotting needle 310 to lower, so as to spot the spotting liquid to the spotting plate. In this way, the structure of the driving assembly 200 is relatively simple, which is convenient for processing and manufacturing.

[0051] When the spotting needle 310 sucks the liquid, the distance of its lowering determines the length of extending into the spotting bottle, so as to determine the maximum amount of the spotting liquid that can be sucked. In addition, the distance of the rising of the piston rod 313 determines the amount of the spotting liquid that is sucked.

[0052] When the spotting needle 310 spots the liquid, and the height difference between the spotting needle 310 and the spotting plate is greater than the desired height difference, the spotting liquid can not be spotted to the spotting plate, or the effect of the spotting is not as expected. When the height difference between the spotting needle 310 and the spotting plate is less than the desired height difference, the spotting needle 310 can contact the spotting plate, which can cause damage to both. Therefore, by accurately controlling the position of the spotting needle 310 to have a desired height difference with the spotting plate, the effect of the spotting can be improved. In addition, the distance of the lowering of the piston rod 313 determines the amount of the spotting liquid for spotting.

[0053] Exemplarily, as Figures 1-4As shown, the main body of the first driving member 210 can be directly or indirectly arranged on the spotting rack 100. In the embodiment in which the spotting device comprises the first horizontal driving member 110 and the second horizontal driving member 120, the main body of the first driving member 210 can be arranged on the driving end of the first horizontal driving member 110. The driving end of the first driving member 210 can be connected to the main body of the second driving member 220, the main body of the third driving member 230 and the cover member 320 for driving the second driving member 220, the third driving member 230, the spotting needle 310 and the cover member 320 to synchronously ascend and descend. The driving end of the second driving member 220 can be connected to the main body of the third driving member 230 and the main body of the spotting needle 310 for driving the third driving member 230 and the spotting needle 310 to synchronously ascend and descend. In this way, the first driving member 210 can simultaneously drive the second driving member 220, the third driving member 230, the spotting needle 310 and the cover member 320 to descend to approach the spotting bottle. After the uncapping portion 322 opens the bottle cap, the second driving member 220 can drive the third driving member 230 and the spotting needle 310 to descend so that the spotting needle 310 can extend into the spotting bottle. Then, the third driving member 230 can drive the piston rod 313 of the spotting needle 310 to ascend, thereby the spotting needle 310 can suck the spotting liquid in the spotting bottle. After the spotting needle 310 finishes sucking the liquid, the second driving member 220 can drive the third driving member 230 and the spotting needle 310 to ascend, thereby the spotting needle 310 can be separated from the spotting bottle. Then, the first driving member 210 can drive the second driving member 220, the third driving member 230, the spotting needle 310 and the cover member 320 to ascend, thereby the uncapping portion 322 and the cover mechanism 400 can be sequentially separated from the bottle cap. In this way, the driving assembly 200 can realize multi-stage driving, thereby the waste of energy can be reduced. Moreover, the second driving member 220 and the third driving member 230 respectively drive the spotting needle 310 and the piston rod 313 to ascend and descend, the stroke can be relatively short, thereby the position of the spotting needle 310 and the piston rod 313 can be precisely controlled by using a structure with high precision, and the amount of the liquid sucked can be precisely controlled. When the spotting is needed, the first driving member 210 and the second driving member 220 can be selected to be started or not according to the position of the spotting plate. For example, when the height difference between the spotting needle 310 and the spotting plate is much larger than the expected height difference, the first driving member 210 can simultaneously drive the second driving member 220, the third driving member 230, the spotting needle 310 and the cover member 320 to descend. Then, the second driving member 220 can drive the third driving member 230 and the spotting needle 310 to descend, so that the spotting needle 310 has the expected height difference with the spotting plate. Then, the third driving member 230 can drive the piston rod 313 of the spotting needle 310 to descend, thereby the spotting liquid can be spotted to the spotting plate. When the height difference between the spotting needle 310 and the spotting plate is slightly larger than the expected height difference, the first driving member 210 can not be started. The second driving member 220 can drive the third driving member 230 and the spotting needle 310 to descend, so that the spotting needle 310 approaches the spotting plate.Then, the third drive member 230 can drive the piston rod 313 of the sampling needle 310 to descend, thereby dispensing the sampling liquid onto the sampling plate. Exemplarily, when the sampling needle 310 and the sampling plate have the expected height difference, neither the first drive member 210 nor the second drive member 220 needs to be activated. The third drive member 230 can directly drive the piston rod 313 of the sampling needle 310 to descend, thereby dispensing the sampling liquid onto the sampling plate. Of course, if the height difference between the sampling needle 310 and the sampling plate is less than the expected height difference, the first drive member 210 and / or the second drive member 220 can also drive the sampling needle 310 to rise, ultimately achieving the expected height difference between the sampling needle 310 and the sampling plate.

[0054] For example, such as Figures 2-4 As shown, the drive assembly 200 may further include a first connecting frame 240, a second connecting frame 250, a third connecting frame 260, a first slide rail 270, and a second slide rail 280. The first connecting frame 240 may be connected between the sample holder 100 and the main body of the first drive member 210. In an embodiment where the sample dispensing device includes a first horizontal drive member 110 and a second horizontal drive member 120, the first connecting frame 240 may be connected between the drive end of the first horizontal drive member 110 and the main body of the first drive member 210. The first slide rail 270 may be disposed on the first connecting frame 240. The second connecting frame 250 may be connected between the drive end of the first drive member 210 and the main body of the second drive member 220. Furthermore, the second connecting frame 250 is slidably connected to the first slide rail 270. The pressure cap 320 and the second slide rail 280 may be disposed on the second connecting frame 250. The third connecting frame 260 may be connected between the drive end of the second drive member 220 and the main body of the third drive member 230. Furthermore, the third connecting frame 260 is slidably connected to the second slide rail 280. The main body of the sampling needle 310 can be mounted on the third connecting frame 260. By setting the first slide rail 270 and the second slide rail 280, the stability and straightness of the lifting and lowering of the cap 320 and the sampling needle 310 can be improved.

[0055] For example, such as Figures 2-4 As shown, the sampling device may further include a sampling needle base 331 and a sampling needle cover 332. The sampling needle base 331 may be mounted on the third connecting bracket 260. The sampling needle cover 332 may be detachably connected to the sampling needle base 331 by any suitable means such as a connector or a snap-fit ​​connection, to hold the main body of the sampling needle 310 (e.g., the needle tube 311 of the sampling needle 310). With this configuration, the sampling needle 310 is easy to install and remove, thus facilitating overall replacement.

[0056] For example, such as Figures 2-4As shown, the driving assembly 200 can further include a first screw rod 291 and a first nut 292. The driving end of the second driving member 220 can be connected to the first screw rod 291 to drive the first screw rod 291 to rotate. The first nut 292 can be connected to the third connecting frame 260. Moreover, the first screw rod 291 can be screwed on the first screw rod 291. The second driving member 220 can drive the first screw rod 291 to rotate, so as to drive the first nut 292 to move up and down along the extension direction of the first screw rod 291, and further drive the third connecting frame 260 and the spotting needle 310 connected to the third connecting frame 260 to move up and down. By arranging the first screw rod 291 and the first nut 292, the position of the spotting needle 310 can be accurately controlled, and the stability of the spotting needle 310 moving up and down is higher. In this way, the accuracy of the spotting needle 310 in sucking liquid and spotting is higher.

[0057] As shown, Figures 2-4 As shown, the driving assembly 200 can further include a second screw rod 293 and a second nut 294. The driving end of the third driving member 230 can be connected to the second screw rod 293 to drive the second screw rod 293 to rotate. The second nut 294 can be directly or through other components connected to the piston rod 313 of the spotting needle 310. Moreover, the second nut 294 can be screwed on the second screw rod 293. The third driving member 230 can drive the second screw rod 293 to rotate, so as to drive the piston rod 313 to move up and down. By arranging the second screw rod 293 and the second nut 294, the position of the piston rod 313 can be accurately controlled, and the stability of the piston rod 313 moving up and down is higher. In this way, the accuracy of the spotting needle 310 in sucking liquid and spotting is higher.

[0058] As shown, Figure 5 As shown, the outer edge of the cover member 320 can be downwardly protruded with a positioning boss 323. Before the cover mechanism 400 presses the bottle cap, the positioning boss 323 can be preferentially sleeved on the top of the bottle cap of the spotting bottle. The positioning boss 323 can be annular or arc-shaped, etc. By arranging the positioning boss 323, the bottle cap can be pre-positioned, so as to facilitate the cover mechanism 400 to accurately press the bottle cap of the spotting bottle, and the spotting needle 310 to accurately extend into the spotting bottle.

[0059] As shown, Figures 2-4As shown, the capping mechanism 400 can include a pressing rod 410 and an elastic member 420. An upper end of the elastic member 420 can be connected to one side of the capping portion 322. A lower end of the elastic member 420 can be connected to the pressing rod 410. The pressing rod 410 can protrude downwardly from the capping portion 322. As the capping member 320 descends, the pressing rod 410 can press the bottle cap, and then can compress the elastic member 420, so as to relatively move towards the capping portion 322. As the descent continues, the capping portion 322 can open the bottle cap. Conversely, as the capping member 320 ascends, the capping portion 322 can be separated from the bottle cap, and the elastic member 420 can release the elastic potential energy to drive the pressing rod 410 to relatively move away from the capping portion 322, so as to continuously press the bottle cap of the sample bottle. As the ascent continues, when the pressing rod 410 is separated from the bottle cap, the elastic member 420 can completely release the elastic potential energy, so as to drive the pressing rod 410 to protrude downwardly from the capping boss, for next use. The elastic member 420 can include, but is not limited to, a spring or an elastic member made of silica gel or the like. In this way, the structure of the capping mechanism 400 is relatively simple, and the manufacturing cost is low.

[0060] As shown in the drawings, Figures 2-4 As shown, the pressing rod 410 can include a pressing portion 411 and an extension rod 412. The extension rod 412 can extend upwardly from the pressing portion 411. The elastic member 420 can be sleeved on the extension rod 412. Moreover, a lower end of the elastic member 420 can be connected to the pressing portion 411. The pressing portion 411 can be used to press and separate the bottle cap of the sample bottle. In this way, the structure of the capping mechanism 400 is relatively compact, and the occupied space is small. Moreover, the extension rod 412 can also play a guiding role on the elastic member 420, so as to avoid the elastic member 420 from deviating from the expected position.

[0061] As shown in the drawings, Figure 5 As shown, the capping member 320 can be provided with a pressing rod through hole 325. The extension rod 412 can pass through the pressing rod through hole 325. In this way, the structure of the capping member 320 and the capping mechanism 400 is relatively compact, and the occupied space is small. Moreover, the pressing rod through hole 325 can also play a guiding role on the extension rod 412, so as to avoid the extension rod 412 from deviating from the expected position.

[0062] As shown in the drawings, the sample device can further include a sample table and a positioning assembly. The sample table can be provided with a groove. The groove can be used to place a sample plate. The positioning assembly can be used to position the sample plate in the groove. The structure of the positioning assembly can be arbitrary, for example, can be a suction cup. In some embodiments, the positioning assembly can include a plurality of air cylinder driving members, which can drive the sample plate in the groove to move, so as to position the sample plate. The sample needle 310 can sample the sample plate in the groove. In this way, the sample device can also perform sample operation, and its function is more abundant.

[0063] Exemplarily, the spotting device can further include a detector and a controller. The controller can be electrically connected to the detector. The detector can be located aside the spotting plate in the recess. The detector can be configured to detect the height of the non-transparent top surface of the spotting plate tilted downward to generate and send a signal to the controller. Generally, the spotting plate can include a transparent body at the bottom and a plating layer at the top of the transparent body. The transparent body can be made of glass or the like to facilitate photographing. The plating layer can include a silica gel plating layer or the like. The top surface of the plating layer can be the non-transparent top surface. The non-transparent top surface can be attached with spreading agent and spotting liquid or the like. When the spotting needle 310 spots, the controller can be configured to make the spotting needle 310 have a desired height difference with the non-transparent top surface based on the signal. The detector can be various types of detectors known in the art or to be developed in the future, including but not limited to a laser sensor or an infrared sensor. The controller can be built with electronic elements such as timers, comparators, registers, digital logic circuits or implemented with processor chips such as single-chip microcomputers, microprocessors, programmable logic controllers (PLC), digital signal processors (DSP), field programmable gate arrays (FPGA), programmable logic arrays (PLA), application-specific integrated circuits (ASIC) and their peripheral circuits.

[0064] In actual application, the detector of the spotting device can send detection light downwardly tilted, and the detection light can be reflected back to the detector by the non-transparent top surface. In this way, the detector can send a signal to the controller. The controller can receive the signal, and thus can calculate the height of the non-transparent top surface. Exemplarily, the controller can take the distance between the non-transparent top surface of a standard spotting plate and the detector as a standard distance, and then can calculate the distance between the currently detected spotting plate and the detector according to the signal, and then can compare the standard distance to calculate the height of the non-transparent top surface of the currently detected spotting plate. In this way, when the spotting needle 310 spots, the controller can be based on the height of the non-transparent top surface, and thus can provide a basis for the spotting needle 310 to have a desired height difference with the non-transparent top surface. Exemplarily, in the embodiment in which the spotting device includes the driving assembly 200, the controller can control the driving assembly 200 to make the spotting needle 310 have a desired height difference with the non-transparent top surface. In this way, the effect of spotting can be improved.

[0065] Due to manufacturing errors, the heights of different spotting plates can be different. The height of the non-transparent top surface of the spotting plate can be calculated by the detector and the controller, and thus the spotting needle 310 can have a better effect of spotting. In this way, the difference of the spotting plates will not cause the effect of spotting to be poor. Moreover, since the detector is located aside the spotting plate in the recess, it can avoid the space above the spotting plate to facilitate the spotting needle 310 to spot and place and take away the spotting plate.

[0066] Exemplarily, the spotting device can further include a reference check block. When the spotting needle 310 is replaced, the lower end of the spotting needle 310 can contact the reference check block, so that the position of the spotting needle 310 is taken as a reference position. In this way, the spotting needle 310 can be driven to lift with the reference position. Exemplarily, in the embodiment in which the spotting device includes the driving assembly 200, the driving assembly 200 can drive the spotting needle 310 to lift with the reference position. In this way, even if there is an installation error when the spotting needle 310 is replaced, the position of the spotting needle 310 can still be accurately controlled.

[0067] In the description of the utility model, it needs to be understood that the orientation words such as '' front'', '' back'', '' upper'', '' lower'', '' left'', '' right'', '' horizontal'', '' vertical'', '' vertical'', '' horizontal'' and '' top'', '' bottom'' and the like indicated orientation or positional relationship is usually based on the orientation or positional relationship shown in the drawing, only for the convenience of describing the utility model and simplifying the description, under the condition that there is no opposite statement, these orientation words do not indicate and imply that the device or element indicated must have a particular orientation or be constructed and operated in a particular orientation, therefore it can not be understood as the limitation to the protection scope of the utility model;The orientation words '' inside'', '' outside'' refer to the inside and outside relative to the outline of each component.

[0068] For the convenience of description, regional relative terms such as '' above'', '' above'', '' upper surface'', '' upper'' and the like can be used here to describe the regional positional relationship of one or more components or features shown in the drawing with other components or features. It should be understood that the regional relative terms not only include the orientation of the components described in the drawing, but also include different orientations in use or operation. For example, if the components in the drawing are inverted as a whole, the components '' above'' or '' above'' other components or features will include the case of '' below'' or '' below'' other components or structures. Thus, the exemplary term '' above'' can include both '' above'' and '' below''. In addition, these components or features can also be positioned at other different angles (for example, rotated by 90 degrees or other angles), and all these cases are intended to be included herein.

[0069] It should be noted that the terms used herein are only for the purpose of describing specific embodiments, and are not intended to limit the exemplary embodiments according to the present application. As used herein, the singular form is intended to include the plural form unless the context clearly indicates otherwise, and furthermore, it should be understood that when the terms '' include '' and / or '' comprise '' are used in the specification, the presence of a feature, step, operation, component, assembly and / or their combinations is indicated.

[0070] It should be noted that the terms "first", "second", and the like in the description and in the claims of the present application and in the above-described drawings are used only for distinguishing between similar objects and do not necessarily have to describe a specific sequential or chronological order. It is to be understood that the data so distinguished can be interchanged, under appropriate circumstances, such that the embodiments of the present application described herein can be practiced in other than the illustrated or described order.

[0071] The utility model has carried on the explanation through the above embodiment, but should understand, the above embodiment is only for example and the purpose of explanation, and not intend to limit the utility model to the range of described embodiment. In addition, the person skilled in the art can understand that the utility model is not limited to the above embodiment, and more kinds of variations and modifications can be made according to the teaching of the utility model, and these variations and modifications all fall within the scope of the utility model claimed. The protection scope of the utility model is defined by the attached claims and its equivalent scope.

Claims

1. A sampling device, characterized in that, include: A sampling assembly includes a sampling needle and a capping member. The capping member includes an opening portion and a capping mechanism disposed on one side of the opening portion. The opening portion has a through hole for the sampling needle to pass through. The sampling assembly is configured such that during liquid aspiration, the sampling needle and the capping member descend, and the capping mechanism presses the cap of the sampling bottle before the opening portion opens the cap, so that the sampling needle passes through the cap and extends into the sampling bottle. After the sampling needle is withdrawn from the sampling bottle after liquid aspiration is completed, the capping mechanism disengages from the cap later than the opening portion.

2. The sampling device as described in claim 1, characterized in that, The opening portion includes an expanding protrusion for expanding the sealing layer on the bottle cap to separate the sealing layer from the sampling needle.

3. The sampling device as described in claim 1, characterized in that, The capping mechanism includes a pressure rod and an elastic element. The upper end of the elastic element is connected to the opening portion of the capping component and the lower end is connected to the pressure rod. The pressure rod is used to press and release the bottle cap. The pressure rod presses the bottle cap before the opening portion opens the bottle cap, so as to compress the elastic element and make the elastic element have elastic potential energy to drive the pressure rod downward to protrude out of the opening portion.

4. The sampling device as described in claim 3, characterized in that, The pressure bar includes a pressing part and an extension rod extending upward from the pressing part, and the elastic element is sleeved on the extension rod and its lower end is connected to the pressing part.

5. The sampling device as described in claim 4, characterized in that, A pressure rod through hole is provided on one side of the opening portion of the pressure cover, and the extension rod passes through the pressure rod through hole.

6. The sampling device as described in claim 1, characterized in that, The outer edge of the capping component has a downward protruding positioning boss, which is used to fit the top of the bottle cap before the capping mechanism presses the bottle cap.

7. The sampling device as described in claim 1, characterized in that, The sampling device further includes a drive assembly connected to the sampling needle and the capping member. The drive assembly is used to drive the sampling needle and the capping member to move up and down, so that the capping mechanism presses and disengages from the bottle cap, the capping part opens the bottle cap, the sampling needle passes through the through hole and extends into the sampling bottle to draw up the sampling liquid in the sampling bottle, and the sampling needle passes through the through hole and disengages from the sampling bottle.

8. The sampling device as described in claim 7, characterized in that, The sampling device further includes a first horizontal drive and a second horizontal drive. The drive end of the first horizontal drive is connected to the body of the drive assembly to drive the drive assembly to move along a first horizontal direction. The drive end of the second horizontal drive is connected to the body of the first horizontal drive to drive the first horizontal drive and the drive assembly to move along a second horizontal direction perpendicular to the first horizontal direction.

9. The sampling device as described in claim 1, characterized in that, The spotting needle includes a needle tube, a needle tip, and a piston rod. The piston rod is movable within the needle tube, and the needle tip is integrally connected to the lower end of the needle tube.

10. The sampling device as described in claim 9, characterized in that, The outer diameter of the needle is between 0.4 mm and 0.6 mm; and / or The inner diameter of the needle is between 0.1mm and 0.3mm.

11. The sampling device as described in claim 1, characterized in that, The sampling device further includes a sampling stage and a positioning component. The sampling stage is provided with a groove for placing a sampling plate. The positioning component is used to position the sampling plate in the groove. The sampling needle is used to apply the sample to the sampling plate.

12. The sampling device as described in claim 11, characterized in that, The spotting device further includes a detector and a controller electrically connected to the detector. The detector is located to the side of the spotting plate and is used to detect the height of the non-transparent top surface of the spotting plate at an angle downwards, so as to generate and send a signal to the controller. The controller is used to make the spotting needle have a expected height difference with the non-transparent top surface when the spotting needle is spotting, based on the signal.

13. The sampling device as described in claim 1, characterized in that, The sampling device further includes a reference calibration block, and the lower end of the sampling needle is used to contact the reference calibration block so that the position of the sampling needle is used as a reference position.

14. A sampling device, characterized in that, Includes the sampling device as described in any one of claims 1-13.

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