Embedding mold for multiple frozen tissues

Abstract

The utility model relates to the technical field of fixation and preservation of biological tissue samples, and provides an embedding mold for multiple frozen tissues, which comprises a base, a plurality of clamping pieces, a plurality of clamping pieces and a plurality of clamping pieces, and is characterized in that the top surface is provided with a pair of centrosymmetric supporting blocks; the sleeve is detachably arranged outside the pair of supporting blocks in a sleeving manner; wherein the pair of supporting blocks are arranged at an interval of 180 degrees, and the outer side walls of the supporting blocks are attached to the inner side wall of the sleeve in a transition fit mode; the sleeve is as high as the supporting block, and an accommodating cavity is defined by the inner side wall of the sleeve and is used for embedding the plurality of strip-shaped frozen tissue blocks in a vertical form. The containing cavity formed in the sleeve is changed into a columnar shape with enough depth from an existing flat shape with limited depth, the problems that the tissue structure is incomplete and an OCT embedding medium overflows are directly solved, the edge of a tissue combined block is smooth, slicing is facilitated, meanwhile, a plurality of strip-shaped frozen tissue blocks are embedded in a vertical mode, and the working efficiency is improved. The height of the tissue block is unified, the size of the tissue block is fixed, and the problems that sample loss is large and tissue arrangement is too close to the edge are solved.

Description

Technical Field

[0001] This utility model relates to the field of biological tissue sample fixation and preservation technology, specifically to an embedding mold for multiple frozen tissues. Background Technology

[0002] Frozen sectioning is a method of rapidly cooling tissue to a certain hardness at low temperatures before sectioning. The method mainly includes tissue sampling, fixation, embedding, sectioning, and staining, with embedding being an essential step. Embedding involves placing the tissue block into an embedding cassette, injecting a certain amount of embedding medium, and then placing the embedding cassette into a cryogenic apparatus for freezing.

[0003] Currently, disposable embedding cassettes are commonly used in laboratories. However, in practical use, these disposable embedding cassettes have the following problems:

[0004] 1. If the mold height is too low, its cavity will be flat and have limited depth, which may easily lead to incomplete individual special structures;

[0005] 2. When embedding multiple tissues at the same time, the OCT embedding agent overflows due to insufficient depth of the mold cavity, resulting in irregular edges of the frozen tissue block and seriously affecting the quality of the section.

[0006] 3. When multiple tissues are embedded at the same time, the height difference between the tissues leads to an uneven top, resulting in significant tissue loss.

[0007] 4. When embedding multiple tissues at the same time, the insufficient width of the mold causes the tissues to be arranged close to the edge, making it difficult to section. Utility Model Content

[0008] To address the shortcomings of existing technologies, this utility model provides an embedding mold for multiple frozen tissues, which solves the problems of insufficient height, width, and cavity depth of disposable embedding boxes in existing technologies.

[0009] To achieve the above objectives, the present invention provides the following technical solution:

[0010] An embedding mold for multiple frozen tissues, comprising:

[0011] The base has a pair of centrally symmetrical support blocks on its top surface; and

[0012] A sleeve, detachably fitted onto a pair of support blocks;

[0013] Among them, a pair of support blocks are spaced apart and arranged at 180° intervals, and their outer sidewalls are fitted together with the inner sidewalls of the sleeve in a transitional fit; the sleeve and the support blocks are at the same height, and their inner sidewalls enclose a cavity for vertically embedding multiple strip-shaped frozen tissue blocks.

[0014] In one embodiment disclosed in this application, an annular groove is provided on the top surface of the base surrounding the pair of support blocks;

[0015] The lower end of the sleeve is detachably embedded in the annular groove.

[0016] In one embodiment disclosed in this application, the sleeve has a circular cross-section and its inner wall is a smooth curved surface;

[0017] The support block has an arc-shaped cross-section, and its inner wall is a smooth plane.

[0018] In one embodiment disclosed in this application, the outer wall of the sleeve is provided with a plurality of raised textures.

[0019] In one embodiment disclosed in this application, the base is square or prismatic in shape to facilitate hand-holding and rotation.

[0020] In one embodiment disclosed in this application, both the base and the sleeve are made of PETG filament combined with 3D printing.

[0021] In one embodiment disclosed in this application, the embedding mold can be reused after being cleaned by soaking in an organic solvent.

[0022] In one embodiment disclosed in this application, the organic solvent is ethanol.

[0023] Compared with the prior art, the beneficial effects of this utility model are:

[0024] 1. The accommodating cavity in the sleeve has been changed from the existing flat shape with limited depth to a column shape with sufficient depth, which directly solves the problems of incomplete tissue structure and OCT embedding agent overflow, making the edges of the tissue block smooth and easy to slice. At the same time, multiple strip-shaped frozen tissue blocks are embedded vertically, which unifies the height of the tissue block and fixes the volume of the tissue block, solving the problems of large sample loss and tissue arrangement too close to the edge.

[0025] 2. The lower end of the sleeve is embedded in an annular groove to form a sealing structure, thereby ensuring that the OCT embedding agent injected into the cavity will not leak before solidification.

[0026] 3. The circular structure and smooth curved surface make the sleeve easy to rotate, thereby using the bow-shaped support block to pry open the OCT embedding agent attached to the inner side wall of the sleeve to detach it, which is conducive to better demolding of the tissue block; the bow-shaped structure and smooth plane allow the support block to better support the tissue block, effectively limiting the embedding range of the tissue block to make the edges of the tissue block regular, while facilitating the rapid demolding of the tissue block.

[0027] 4. Several raised lines can increase the friction of the outer wall of the sleeve, making it easier to rotate when holding the sleeve. Attached Figure Description

[0028] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0029] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0030] Figure 2 This is a schematic diagram of the three-dimensional structure of the base;

[0031] Figure 3 This is a schematic diagram of the three-dimensional structure of the sleeve. Detailed Implementation

[0032] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of this invention. Therefore, the drawings and description are considered exemplary in nature and not restrictive.

[0033] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0034] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0035] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. For those skilled in the art, the specific meaning of the above terms in this utility model can be understood according to the specific circumstances.

[0036] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0037] The following disclosure provides many different embodiments or examples for implementing various structures of this invention. To simplify the disclosure, specific examples of components and arrangements are described below. Of course, these are merely examples and are not intended to limit the scope of this invention.

[0038] The embodiments of this utility model will now be described in detail with reference to the accompanying drawings.

[0039] See Figures 1-3 As shown, this utility model provides an embedding mold for multiple frozen tissues, comprising:

[0040] The base 10 has a pair of centrally symmetrical support blocks 11 on its top surface; and

[0041] Sleeve 20 is detachably fitted onto a pair of support blocks 11;

[0042] Among them, a pair of support blocks 11 are spaced apart and arranged at 180° intervals, and their outer sidewalls are fitted together with the inner sidewalls of the sleeve 20 in a transitional fit; the sleeve 20 and the support blocks 11 are at the same height (i.e., their top surfaces are flush), and their inner sidewalls (i.e., the sleeve 20 and the support blocks 11) enclose a cavity for vertically embedding multiple strip-shaped frozen tissue blocks.

[0043] In the process of use, the sleeve 20 is first fitted onto a pair of support blocks 11 to form a receiving cavity. Then, an appropriate amount of OCT embedding agent is poured into the receiving cavity. Multiple prepared single-strip frozen tissue blocks are then placed into the receiving cavity containing OCT embedding agent in sequence and arranged neatly. Then, sufficient OCT embedding agent is added to the receiving cavity to completely cover the multiple single-strip frozen tissue blocks. Finally, the entire embedding mold along with the multiple single-strip frozen tissue blocks is immersed in liquid nitrogen for rapid freezing. Once the OCT embedding agent has solidified and hardened, the sleeve 20 can be removed to achieve demolding. In other words, the receiving cavity in the sleeve 20 has changed from the existing flat shape with limited depth to a columnar shape with sufficient depth, directly solving the problems of incomplete tissue structure and OCT embedding agent overflow. This makes the edges of the tissue block smooth, which is convenient for slicing. At the same time, embedding multiple strip frozen tissue blocks vertically unifies the height and fixes the volume of the tissue block, solving the problems of large sample loss and tissue arrangement too close to the edge.

[0044] An annular groove 12 is provided on the top surface of the base 10 surrounding the pair of support blocks 11, and the lower end of the sleeve 20 is detachably embedded in the annular groove 12. In this way, the lower end of the sleeve 20 embedded in the annular groove 12 can form a sealing structure, thereby ensuring that the OCT embedding agent injected into the accommodating cavity will not leak before solidification.

[0045] The sleeve 20 has a circular cross-section with a smooth curved inner wall; the support block 11 has an arc-shaped cross-section with a smooth flat inner wall. The circular structure and smooth curved surface facilitate rotation of the sleeve 20, allowing the arc-shaped support block 11 to pry open the OCT embedding agent adhering to the inner wall of the sleeve 20, thus facilitating better demolding of the tissue assembly. The arc-shaped structure and smooth flat surface also allow the support block 11 to better support the tissue assembly, effectively limiting the embedding area and ensuring regular edges, while also facilitating rapid demolding. Specifically, during demolding, the base 10 is held while the sleeve 20 is rotated (or the sleeve 20 is held while the base 10 is rotated), causing the OCT embedding agent in the cavity to detach from the sleeve 20 first. Then, the tissue assembly is pushed from the side along a direction parallel to the inner wall of the support block 11, thereby achieving demolding.

[0046] To facilitate gripping or rotating the sleeve 20, several raised textures (not shown in the figure) are provided on the outer wall of the sleeve 20. These raised textures increase the friction of the outer wall of the sleeve 20, making it easier to rotate when gripping the sleeve 20.

[0047] See Figure 1 As shown, the base 10 has a square or prismatic structure to facilitate hand-holding and rotation.

[0048] Both the base 10 and the sleeve 20 are made using PETG filament and 3D printing. 3D printing is simple and low-cost, and can be mass-produced.

[0049] This embedding mold can be reused after being cleaned by soaking in an organic solvent. In this embodiment, ethanol is preferred as the organic solvent.

[0050] The above embodiments are merely preferred embodiments of this utility model and are not intended to limit the technical solutions of this utility model. Any technical solution that can be implemented based on the above embodiments without creative effort should be considered to fall within the scope of protection of this utility model patent.

Claims

1. A multi-frozen tissue embedding mold, characterized by, The application relates to a multiple-ice-frozen-tissue embedding mold, which comprises a base and a sleeve. The base is provided with a pair of center-symmetrical supporting blocks on the top surface; and the sleeve is detachably sleeved on the pair of supporting blocks. The pair of supporting blocks are spaced apart and arranged at intervals of 180 degrees, and the outer side wall of the supporting blocks is matched with the inner side wall of the sleeve in a transition mode. The sleeve is in the same height with the supporting blocks, and the inner side wall of the sleeve is enclosed to form a containing cavity for vertically embedding a plurality of strip-shaped ice-frozen-tissue blocks.

2. The multiple-ice-frozen-tissue embedding mold according to claim 1, wherein: a ring-shaped clamping groove is formed on the top surface of the base around the pair of supporting blocks; and the lower end of the sleeve is detachably embedded in the ring-shaped clamping groove.

3. The multiple-ice-frozen-tissue embedding mold according to claim 2, wherein: the cross section of the sleeve is in a circular ring structure, and the inner side wall of the sleeve is a smooth curved surface; the cross section of the supporting block is in an arc structure, and the inner side wall of the supporting block is a smooth plane; a plurality of convex lines are arranged on the outer side wall of the sleeve; the base is in a square or prismatic structure, so as to be convenient for holding and rotating; the base and the sleeve are both made of PETG consumables combined with 3D printing; the embedding mold can be repeatedly used after being soaked and cleaned by an organic solvent; and the organic solvent is ethanol. The application relates to a multiple-ice-frozen-tissue embedding mold, which comprises a base and a sleeve. The base is provided with a pair of center-symmetrical supporting blocks on the top surface; and the sleeve is detachably sleeved on the pair of supporting blocks. The pair of supporting blocks are spaced apart and arranged at intervals of 180 degrees, and the outer side wall of the supporting blocks is matched with the inner side wall of the sleeve in a transition mode. The sleeve is in the same height with the supporting blocks, and the inner side wall of the sleeve is enclosed to form a containing cavity for vertically embedding a plurality of strip-shaped ice-frozen-tissue blocks.

4. The multi-ice frozen tissue embedding mold according to any one of claims 1 to 3, characterized by, 2. The multiple-ice-frozen-tissue embedding mold according to claim 1, wherein: a ring-shaped clamping groove is formed on the top surface of the base around the pair of supporting blocks; and the lower end of the sleeve is detachably embedded in the ring-shaped clamping groove.

5. The multi-ice frozen tissue embedding mold of claim 1, wherein, 3. The multiple-ice-frozen-tissue embedding mold according to claim 2, wherein: the cross section of the sleeve is in a circular ring structure, and the inner side wall of the sleeve is a smooth curved surface; the cross section of the supporting block is in an arc structure, and the inner side wall of the supporting block is a smooth plane; a plurality of convex lines are arranged on the outer side wall of the sleeve; the base is in a square or prismatic structure, so as to be convenient for holding and rotating; the base and the sleeve are both made of PETG consumables combined with 3D printing; the embedding mold can be repeatedly used after being soaked and cleaned by an organic solvent; and the organic solvent is ethanol.

6. The multi-ice frozen tissue embedding mold according to claim 1 or 5, wherein ​ 7. The multi-ice frozen tissue embedding mold of claim 1, wherein, ​ 8. The multi-ice frozen tissue embedding mold of claim 7, wherein, ​

Images