Kit for extracting and purifying cell exosome
By improving the sealing structure and UV lamp plate design of the reagent kit, the problem of poor sealing in traditional reagent kits has been solved, achieving effective sealing and inactivation of exosome mother liquor, thus ensuring the safety and convenience of using the reagent kit.
Patent Information
- Application Number
- CN202422548716.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-22
- Publication Date
- 2025-11-25
- Estimated Expiration
- 2034-10-22
AI Technical Summary
Traditional reagent kits have poor sealing properties, which makes it easy for exosome mother fluid to leak.
A reagent kit comprising a sealing cap, a reagent kit body, a storage tube, a cover plate, and a UV lamp plate was designed. The sealing performance is improved by using a sealing strip and a locking column structure, and the UV lamp plate is used to inactivate residual exosomes in the storage tube.
The kit's sealing performance has been improved, preventing leakage of the exosome mother fluid. Residues are inactivated by ultraviolet light, facilitating the reuse of storage tubes.
Smart Images
Figure CN223591311U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the field of cell exosome, especially a kit for cell exosome extraction and purification. BACKGROUND
[0002] Cell exosome is a kind of micro membrane bubble that can be secreted by most cells, has lipid bilayer membrane structure, contains cell-specific protein, lipid and nucleic acid in this micro membrane bubble, can be transferred to other cells as signal molecule to change the function of other cells, with this discovery, cell exosome plays a vital role in many physiology and pathology, when preparing cell exosome, cell exosome mother liquor is usually stored in kit, to facilitate staff to extract and purify mother liquor.
[0003] With the use of the kit in the prior art, it is found that the conventional kit has poor sealing performance, and the cell exosome mother liquor extracted and purified in the kit is prone to leakage. In view of the above problems, the present application provides a kit for cell exosome extraction and purification to solve the problems in the prior art. UTILITY MODEL CONTENT
[0004] In view of the above-mentioned shortcomings of the prior art, the utility model aims to provide a kit for cell exosome extraction and purification to solve the problems in the prior art.
[0005] To achieve the above-mentioned purposes and other related purposes, the utility model provides a kit for cell exosome extraction and purification, which comprises a sealing cover, a kit body, two storage tubes, two cover plates and two ultraviolet lamp plates. The kit body is arranged below the sealing cover, a storage groove is formed in the middle of the top end of the kit body, the bottom ends of the two storage tubes are rotatably connected to the two sides of the bottom end of the storage groove, the two cover plates are respectively connected to the top ends of the two storage tubes, and the two ultraviolet lamp plates are respectively arranged between the two sides of the storage groove. The storage tube is made of light-transmitting material. After the cell exosome storage solution in the storage tube is extracted and purified, the ultraviolet lamp plates are powered on, and the ultraviolet light emitted by the ultraviolet lamp plates inactivates the residual cell exosome in the storage tube.
[0006] Preferably, the surface of the sealing cover is connected with a sealing strip, and four corners of the bottom end of the sealing cover are fixedly provided with clamping columns. The utility model seals the connection between the sealing cover and the kit body by providing a sealing strip, thereby improving the sealing performance of the kit body.
[0007] More preferably, four corners of the top end of the kit body are provided with clamping grooves, and the kit body is connected with the four clamping columns through the clamping grooves. The clamping grooves and the clamping columns are correspondingly arranged, so that the sealing cover can be easily installed on the kit body.
[0008] Preferably, it further comprises two driven gears, two driven gears are fixedly installed at the connection between the storage tube and the storage tank; a micro motor, a micro motor is fixedly installed at the middle of the bottom end of the kit body; a driving gear, the driving gear is rotatably connected to the inner middle part of the storage tank and is driven to rotate by the micro motor; and four supporting columns, the supporting columns are fixedly installed at the four corners of the bottom end of the kit body, the micro motor, the driving gear and the two driven gears are provided, the driven gears rotate under the driving of the driving gear, and the two driven gears drive the storage tube to rotate, so that the used storage tube is convenient for the staff to clean.
[0009] Preferably, the outer sides of the two driven gears are meshed and connected with the outer side of the driving gear, and the driving gear is in contact with the driven gear when rotating.
[0010] Preferably, a plurality of taking grooves are formed in the surface of the kit body, and the taking grooves increase the contact area between the kit body and the palm of the staff, so that the staff can take the kit body conveniently.
[0011] From the above, the beneficial effects of the present application are as follows: the sealing cover, the kit body and the cover plate are used to ensure the sealing property of the cell exosome mother liquor in the storage tube, so that the leakage of the cell exosome mother liquor in the storage tube is avoided; the ultraviolet lamp plate is used to irradiate and inactivate the residual cell exosome mother liquor in the used storage tube, so that the staff can use the storage tube again. BRIEF DESCRIPTION OF DRAWINGS
[0012] In order to more clearly illustrate the technical solutions in the one or more embodiments of the present application or the prior art, the drawings needed in the embodiment or prior art description will be briefly introduced below. Obviously, the drawings in the following description are only one or more embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.
[0013] Fig. 1 It is a side view of the present application;
[0014] Fig. 2 It is a perspective view of the kit body in the present application;
[0015] Fig. 3 It is a sectional view of the kit body in the present application.
[0016] In the drawing: 1. Kit body; 2. Support column; 3. Micro motor; 4. Sealing cover; 5. Sealing strip; 6. Clamping column; 7. Taking groove; 8. Clamping groove; 9. Cover plate; 10. Storage tube; 11. Ultraviolet lamp plate; 12. Driven gear; 13. Driving gear; 14. Storage groove. DETAILED DESCRIPTION
[0017] In order to make the purpose, technical scheme and advantages of the present disclosure clearer, the present disclosure will be further described in detail below in combination with specific embodiments.
[0018] It should be noted that, unless otherwise defined, the technical terms or scientific terms used in one or more embodiments of the present disclosure should be understood as the usual meaning understood by those skilled in the art to which the present disclosure belongs. The terms "first", "second" and the like used in one or more embodiments of the present disclosure do not represent any order, number or importance, but are only used to distinguish different components. The terms "include" or "contain" and the like mean that the elements or objects before the terms cover the elements or objects listed after the terms and their equivalents, and do not exclude other elements or objects. The terms "connect" or "connect" and the like are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to represent relative positional relationships, and when the absolute position of the described object changes, the relative positional relationship may also change accordingly.
[0019] EMBODIMENT
[0020] Please refer to Figs. 1-3 A kit for extracting and purifying extracellular vesicles, comprising a sealing cover 4, a kit body 1, two storage tubes 10, two storage tubes 10, two cover plates 9 and two ultraviolet lamp plates 11, the kit body 1 is arranged below the sealing cover 4, the middle of the top end of the kit body 1 is provided with a storage groove 14, the bottom end of the two storage tubes 10 is rotatably connected with the two sides of the bottom end of the storage groove 14, the two cover plates 9 are respectively threadedly connected with the top end of the two storage tubes 10, the two ultraviolet lamp plates 11 are respectively arranged between the two sides of the storage groove 14, and are used for sterilizing the storage tube 10, the storage tube 10 is made of light-transmitting material, after the extracellular vesicle storage solution in the storage tube 10 is extracted and purified, the ultraviolet lamp plate 11 is powered on, and the ultraviolet light emitted by the ultraviolet lamp plate 11 inactivates the residual extracellular vesicles in the storage tube 10.
[0021] As the improvement scheme of the above scheme, the surface of the sealing cover 4 is connected with a sealing strip 5, and four corners of the bottom end of the sealing cover 4 are fixedly provided with clamping columns 6.
[0022] As the improvement scheme of the above scheme, four corners of the top end of the reagent box body 1 are provided with clamping grooves 8, and the reagent box body 1 is connected with the four clamping columns 6 through the clamping grooves 8, the clamping grooves 8 and the clamping columns 6 are correspondingly arranged, and the sealing cover 4 is conveniently installed on the reagent box body 1.
[0023] As the improvement scheme of the above scheme, the utility model also includes two driven gears 12, the connecting parts between the two storage tubes 10 and the storage grooves 14 are fixedly provided with the driven gears 12, a micro motor 3 is fixedly installed at the middle part of the bottom end of the reagent box body 1, a driving gear 13 is rotatably connected to the inner middle part of the storage groove 14 and is driven to rotate by the micro motor 3, and four supporting columns 2 are fixedly installed at the four corners of the bottom end of the reagent box body 1, the micro motor 3, the driving gear 13 and the two driven gears 12 are arranged, the driven gears 12 are driven to rotate by the driving gear 13, the two driven gears 12 drive the storage tubes 10 to rotate, and the used storage tubes 10 are conveniently cleaned by the staff.
[0024] As the improvement scheme of the above scheme, the outer sides of the two driven gears 12 are rotatably connected with the outer side of the driving gear 13, and the driving gear 13 is in contact with the driven gears 12 when rotating.
[0025] As the improvement scheme of the above scheme, the surface of the reagent box body 1 is provided with a plurality of taking grooves 7, the taking grooves 7 increase the contact area between the reagent box body 1 and the palm of the staff, and the staff can conveniently take the reagent box body 1.
[0026] Work flow: the staff stores the mother liquor of the cell exosome in the storage tube 10, rotates the cover plate 9, the threads on the surface of the cover plate 9 are matched with the threads on the inner wall of the storage tube 10, so the staff seals the storage tube 10 by rotating the cover plate 9, aligns the clamping columns 6 with the clamping grooves 8, and clamps and installs the sealing cover 4 on the reagent box body 1, the sealing strip 5 seals the connecting part between the sealing cover 4 and the reagent box body 1, improves the sealing property of the reagent box body 1, the storage tube 10 is made of a light-transmitting material, after the cell exosome storage solution in the storage tube 10 is extracted and purified, the staff electrifies the ultraviolet lamp plate 11, and the ultraviolet light emitted by the ultraviolet lamp plate 11 inactivates the residual cell exosome in the storage tube 10.
[0027] The above embodiments only exemplarily illustrate the principles and effects of the present application, and are not used to limit the present application. Any person skilled in the art can modify or change the above embodiments without departing from the spirit and scope of the present application. Therefore, all equivalent modifications or changes completed by those skilled in the art without departing from the spirit and technical thought disclosed by the present application should be covered by the claims of the present application.
Claims
1. A kit for extracting and purifying extracellular vesicles, characterized by, Include: Sealing cover (4); The kit body (1) is arranged below the sealing cover (4), and the middle part of the top end of the kit body (1) is provided with a storage groove (14); Two storage tubes (10), the bottom end of the two storage tubes (10) is rotatably connected with the two sides of the bottom end of the storage groove (14); Two cover plates (9), two storage tubes (10) are connected with the top end of the two cover plates (9); And Two ultraviolet lamp plates (11), two ultraviolet lamp plates (11) are respectively arranged between the two sides of the storage groove (14).
2. The kit for extracting and purifying extracellular vesicles according to claim 1, characterized in that, The surface of the sealing cover (4) is connected with a sealing strip (5), and the four corners of the bottom end of the sealing cover (4) are fixedly installed with clamping columns (6). 3.The kit for extracting and purifying extracellular vesicles according to claim 1, characterized in that, The four corners of the top end of the kit body (1) are provided with clamping grooves (8), and the kit body (1) is connected with the four clamping columns (6) through the clamping grooves (8).
4. The kit for extracting and purifying extracellular vesicles according to claim 1, characterized in that, Also include: Two driven gears (12), the connecting parts of the two storage tubes (10) and the storage groove (14) are fixedly installed with driven gears (12); Micro motor (3), the middle part of the bottom end of the kit body (1) is fixedly installed with a micro motor (3); Driving gear (13), the inner middle part of the storage groove (14) is rotatably connected with the driving gear (13), and the driving gear (13) is driven to rotate by the micro motor (3); And Four support columns (2), the four corners of the bottom end of the kit body (1) are fixedly installed with support columns (2).
5. The kit for extracting and purifying extracellular vesicles according to claim 4, characterized in that, The outer sides of the two driven gears (12) are rotatably connected with the outer sides of the driving gear (13).
6. The kit for extracting and purifying extracellular vesicles according to claim 1, characterized in that, The surface of the kit body (1) is provided with a plurality of taking grooves (7).