Gene chip hybridization box

Abstract

The utility model relates to the technical field of biology, and discloses a gene chip hybridization box which comprises a box body, the inner bottom wall of the box body is fixedly connected with a base plate, the inside of the base plate is slidably connected with two groups of limiting components, one side of each limiting component is clamped with a gene chip main body, and the inner bottom wall of the box body is fixedly connected with four groups of fixing seats. According to the gene chip hybridization box, through the arrangement of the limiting assembly, when the gene chip hybridization box is used, a gene chip main body is clamped between two groups of clamping plates, and the distance between the two groups of clamping plates is slightly smaller than the length of the gene chip main body, so that a telescopic spring is stressed and deformed to generate elastic force, and a sliding column slides in a base plate for limiting; the telescopic spring pulls the clamping plate to clamp and fix the gene chip main body in order to recover deformation, so that the effect of stably clamping the gene chip main body is achieved, the gene chip main body is prevented from deviating or being unstable in the detection process, and the stability and accuracy of the gene chip hybridization box are improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biotechnology especially, relate to a gene chip hybridization box. BACKGROUND

[0002] Gene chip is the detection method that is based on gene linkage, restriction length polymorphism and linkage disequilibrium etc. gene mapping method, and is based on homologous DNA molecule hybridization as the basic working principle and designs the sequencing principle of gene chip is hybridization sequencing method, that is the method for determining nucleic acid sequence through hybridization with a group of known sequence nucleic acid probe, when the nucleic acid sequence TATGCAATCTAG with fluorescent label in solution, with nucleic acid probe of corresponding position on gene chip produces complementary matching, through determining the probe position of strongest fluorescence intensity, obtains a group of sequence completely complementary probe sequence. The main role of a kind of gene chip hybridization box is used in sample hybridization process in gene chip experiment, help analysis and detection gene expression, variation or other genome information, gene chip hybridization box is the important tool in molecular biology research and gene expression analysis, can help scientific research personnel to accurately obtain and analyze gene information.

[0003] The existing gene chip hybridization box is not convenient to stably clamp the gene chip main body during actual use, and the gene chip main body is easy to deviate or unstable during detection.

[0004] Through the search of existing patents: gene chip hybridization box and gene chip set (publication number: CN212375275U), the gene chip hybridization box includes: a substrate having a gene chip arrangement area, a first surface of the gene chip can be joined to the gene chip arrangement area; a cover plate provided with a through hole, the through hole is opposite to the gene chip arrangement area; a spacer provided between the substrate and the cover plate, which generates a predetermined interval between the substrate and the cover plate, and the spacer has an opening in the area facing at least one gene chip arrangement area, thereby defining a gene chip containing space between the substrate and the cover plate; the predetermined interval is set so that when the gene chip is fixed in the gene chip arrangement area, the second surface of the gene chip opposite to the first surface and including the probe region is spaced from the surface of the cover plate facing the second surface between 0.05mm and 0.15mm, so that when the hybridization solution is injected through the through hole of the cover plate, the hybridization solution can be evenly distributed on the second surface by capillary action.

[0005] The above-mentioned patent can make the hybridization solution evenly distributed on the second surface by capillary action when the hybridization solution is injected through the through hole of the cover plate, but it is not convenient to quickly close the gene chip hybridization box, and it is difficult to maintain sealing.

[0006] Therefore, it is necessary to invent a gene chip hybridization box to solve the above-mentioned problems. The utility model discloses a gene chip hybridization box

[0007] (I) the technical problem solved

[0008] The utility model discloses a gene chip hybridization box, which is high in practicability, simple in structure and easy to operate, and solves the problems of unstable clamping of the gene chip main body and inconvenient quick closure of the gene chip hybridization box in the prior art.

[0009] (II) Technical scheme

[0010] To achieve the above object, the utility model discloses the following technical scheme: a gene chip hybridization box, including the box body, the inner bottom wall of box body is fixedly connected with the base plate, the inside of base plate is slidably connected with two groups of limit components, one side of limit component is connected with gene chip main body, the inner bottom wall of box body is fixedly connected with four groups of fixed seat, the inside of fixed seat is inserted with the limit post, the top of limit post is fixedly connected with the cover plate, the inside of box body is connected with the sealing ring, the surface of box body is fixedly connected with the support frame, both sides of support frame are connected with the closure component, the bottom of closure component is fixedly connected with the fixed frame, and the inside of fixed frame is fixedly connected with the box cover.

[0011] As a further scheme of the utility model, the limit component includes two groups of sliding columns slidably connected in the inside of the base plate, one end of the sliding column is fixedly connected with the clamping plate, one side of the clamping plate is fixedly connected with two groups of telescopic springs, and the telescopic spring is convenient for pulling the clamping plate.

[0012] As a further scheme of the utility model, the closure component includes a clamping strip clamped on both sides of the support frame, and one end of the clamping strip is rotatably connected with a rotating bolt, and the rotating bolt is convenient for rotating.

[0013] As a further scheme of the utility model, the surface of the gene chip main body is adhesively connected with a hybridization fence, the bottom surface of the cover plate is fixedly connected with a plurality of cavities matched with the hybridization fence, and the surface of the cover plate is provided with a plurality of injection holes, and the injection hole is convenient for injecting the fluorescent solvent.

[0014] As a further scheme of the utility model, the top surface of the box body is provided with a clamping groove matched with the sealing ring, and the inside of the base plate is provided with a sliding groove matched with the clamping plate, and the sliding groove is convenient for sliding the clamping plate.

[0015] As a further scheme of the utility model, the inside of the fixed seat is provided with a positioning hole matched with the limit post, and the top of the box cover is fixedly connected with an observation window, and the observation window is convenient for observing the internal hybridization.

[0016] As a further scheme of the utility model, the recess is arranged on one side of the clamping strip, and the rotating hole adapted to the rotating bolt is arranged on the top of the clamping strip, so that the clamping strip can be conveniently rotated.

[0017] (Three) beneficial effects

[0018] The utility model provides a gene chip hybridization box has the following beneficial effects:

[0019] 1、the gene chip hybridization box, through the setting of limiting component, in use, the gene chip main part is clamped into two groups of clamping plates, because the distance between two groups of clamping plates is slightly less than the length of gene chip main part, make that telescopic spring force deformation produces elasticity, and then sliding column is limited in the sliding of base plate, telescopic spring is restored to change and drive clamping plate and be clamped and fixed gene chip main part, thereby reach the effect of stable clamping gene chip main part, prevent the deviation or instability of gene chip main part in the detection process, improve the stability and accuracy of gene chip hybridization box.

[0020] 2、the gene chip hybridization box, through the setting of closing assembly and sealing ring, in use, the sealing ring is clamped in the clamping groove on the box body, and then the box cover is covered on the box body, the box cover is pressed, so that the fixed frame and the supporting frame are tightly attached, and then the clamping strip is rotated, so that the clamping strip is rotated on the rotating bolt, and the fixed frame and the supporting frame are clamped into the clamping strip, so that the effect of conveniently closing the gene chip hybridization box is achieved, the steps of closing the gene chip hybridization box are reduced, and the sealing property and convenience of the gene chip hybridization box are improved. BRIEF DESCRIPTION OF DRAWINGS

[0021] Fig. 1 It is the whole structure schematic diagram of the utility model;

[0022] Fig. 2 It is the split structure schematic diagram of the utility model;

[0023] Fig. 3 It is the limiting component structure schematic diagram of the utility model;

[0024] Fig. 4 It is the closing assembly structure schematic diagram of the utility model.

[0025] In the drawing: 1, box body;2, base plate;3, limiting component;301, sliding column;302, clamping plate;303, telescopic spring;4, gene chip main part;5, fixed seat;6, limiting column;7, cover plate;8, sealing ring;9, supporting frame;10, closing assembly;1001, clamping strip;1002, rotating bolt;11, fixed frame;12, box cover;13, hybridization fence;14, observation window. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Based on the embodiments in the utility model, all other embodiments obtained by the person skilled in the art without creative labor fall within the protection scope of the utility model.

[0027] Please refer to Figs. 1 to 4 The utility model provides a kind of technical scheme: a gene chip hybridization box, the inner bottom wall of box body 1 is fixedly connected with substrate 2, two groups of limiting components 3 are slidably connected in substrate 2, by the setting of limiting component 3, reach the effect of stable clamping gene chip main body 4, prevent gene chip main body 4 from deviating or being unstable during detection, improve the stability and accuracy of gene chip hybridization box, one side of limiting component 3 is clamped with gene chip main body 4, the inner bottom wall of box body 1 is fixedly connected with four groups of fixed seat 5, limiting column 6 is inserted in fixed seat 5, the top of limiting column 6 is fixedly connected with cover plate 7, the inside of box body 1 is clamped with sealing ring 8, the surface of box body 1 is fixedly connected with support frame 9, the two sides of support frame 9 are clamped with closure component 10, by the setting of closure component 10 and sealing ring 8, reach the effect of convenient closure gene chip hybridization box, reduce the steps of closure gene chip hybridization box, improve the sealing property and convenience of gene chip hybridization box, the bottom of closure component 10 is fixedly connected with fixed frame 11, fixed frame 11 is fixedly connected with box cover 12 in the inside.

[0028] Limiting component 3 includes two groups of sliding columns 301 slidably connected in the inside of substrate 2, one end of sliding column 301 is fixedly connected with clamping plate 302, one side of clamping plate 302 is fixedly connected with two groups of telescopic springs 303, by the setting of telescopic spring 303, play the role of pulling clamping plate 302.

[0029] Closure component 10 includes clamping strip 1001 clamped in the two sides of support frame 9, one end of clamping strip 1001 is rotatably connected with rotating bolt 1002, by the setting of rotating bolt 1002, play the role of facilitating the rotation of rotating bolt 1002.

[0030] The surface of gene chip main body 4 is adhesively connected with hybridization fence 13, the bottom of cover plate 7 is fixedly connected with several groups of cavities matched with hybridization fence 13, several groups of injection holes are formed in the surface of cover plate 7, by the setting of injection hole, play the role of facilitating the injection of fluorescent solvent.

[0031] The top surface of box body 1 is provided with clamping groove matched with sealing ring 8, the inside of substrate 2 is provided with sliding groove matched with clamping plate 302, by the setting of sliding groove, play the role of facilitating the sliding of clamping plate 302.

[0032] The inside of the fixed seat 5 is provided with a positioning hole matched with the limiting column 6, and the top of the box cover 12 is fixedly connected with an observation window 14.

[0033] The card strip 1001 is provided with a groove on one side, and the top of the card strip 1001 is provided with a rotating hole matched with the rotating bolt 1002, so that the card strip 1001 is conveniently rotated.

[0034] The working steps of the device are as follows:

[0035] The first step is that, in use, the gene chip main body 4 is clamped into the middle of the two sets of clamping plates 302, and since the distance between the two sets of clamping plates 302 is slightly smaller than the length of the gene chip main body 4, the elastic force is generated by the stress deformation of the extension spring 303;

[0036] The second step is that the sliding column 301 is limited by sliding in the inside of the base plate 2, and the extension spring 303 pulls the clamping plate 302 to clamp and fix the gene chip main body 4 for restoring the deformation;

[0037] The third step is that, in use, the sealing ring 8 is clamped in the clamping groove on the box body 1, and then the box cover 12 is covered on the box body 1, the box cover 12 is pressed, the fixed frame 11 and the supporting frame 9 are tightly attached, and then the card strip 1001 is rotated, so that the card strip 1001 is rotated on the rotating bolt 1002, and the fixed frame 11 and the supporting frame 9 are clamped into the card strip 1001.

[0038] It should be noted that the device structure and the drawings of the utility model mainly describe the principle of the utility model, and the setting of the power mechanism, the power supply system and the control system of the device is not completely described in the technical principle of the design, and under the premise that the above-mentioned principle of the utility model is understood by the person skilled in the art, the specific power mechanism, power supply system and control system can be clearly known, and the control mode of the application file is automatically controlled by the controller, and the control circuit of the controller can be realized by simple programming of the person skilled in the art;

[0039] The standard parts used can be purchased from the market, and can be ordered according to the description and drawings, the specific connection mode of each part adopts the conventional bolt, rivet, welding and other conventional means in the prior art, the machinery, parts and equipment adopt the conventional type in the prior art, and the components known by the person skilled in the art, and the structure and principle thereof are known by the person skilled in the art through technical manual or conventional experimental method.

[0040] Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A gene chip hybridization cartridge comprising a cartridge body (1), characterized in that: The inner bottom wall of the box body (1) is fixedly connected with a base plate (2), the inner part of the base plate (2) is slidably connected with two groups of limiting assemblies (3), one side of the limiting assembly (3) is clamped with a gene chip body (4), the inner bottom wall of the box body (1) is fixedly connected with four groups of fixed seats (5), the inner part of the fixed seat (5) is inserted with a limiting column (6), the top of the limiting column (6) is fixedly connected with a cover plate (7), the inner part of the box body (1) is clamped with a sealing ring (8), the surface of the box body (1) is fixedly connected with a supporting frame (9), the two sides of the supporting frame (9) are clamped with a closing assembly (10), the bottom of the closing assembly (10) is fixedly connected with a fixed frame (11), the inner part of the fixed frame (11) is fixedly connected with a box cover (12).

2. The gene chip hybridization cartridge of claim 1, wherein: The limiting assembly (3) includes two groups of sliding columns (301) slidably connected in the inner part of the base plate (2), one end of the sliding column (301) is fixedly connected with a clamping plate (302), one side of the clamping plate (302) is fixedly connected with two groups of extension springs (303).

3. The gene chip hybridization cartridge of claim 1, wherein: The closing assembly (10) includes a clamping strip (1001) clamped on the two sides of the supporting frame (9), one end of the clamping strip (1001) is rotatably connected with a rotating bolt (1002).

4. The gene chip hybridization cartridge of claim 1, wherein: The surface of the gene chip body (4) is adhesively connected with a hybrid fence (13), the bottom surface of the cover plate (7) is fixedly connected with a plurality of cavities matched with the hybrid fence (13), and the surface of the cover plate (7) is provided with a plurality of injection holes.

5. The gene chip hybridization cartridge of claim 1, wherein: The top surface of the box body (1) is provided with a clamping groove matched with the sealing ring (8), and the inner part of the base plate (2) is provided with a sliding groove matched with the clamping plate (302).

6. The gene chip hybridization cartridge of claim 1, wherein: The inner part of the fixed seat (5) is provided with a positioning hole matched with the limiting column (6), and the top of the box cover (12) is fixedly connected with an observation window (14).

7. The gene chip hybridization cartridge of claim 3, wherein: One side of the clamping strip (1001) is provided with a groove, and the top of the clamping strip (1001) is provided with a rotating hole matched with the rotating bolt (1002).

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