Multi-unit modularized full-automatic nucleic acid amplification analysis system

Abstract

The utility model relates to a multi-unit modularized full-automatic nucleic acid amplification analysis system, which can realize on-site multi-channel detection and improve the detection efficiency, and comprises a base, the top of the base is provided with an accommodating cavity, a plurality of mounting stations are formed in the accommodating cavity, each mounting station is internally and removably provided with a reaction module, and the reaction modules are arranged in the accommodating cavity. The inner wall of the containing cavity is provided with electrical interfaces corresponding to the installation stations, the electrical interfaces are used for being electrically connected with the corresponding reaction modules, and the base is further provided with a power interface or a power module connected with the electrical interfaces. The reaction modules are used for performing nucleic acid extraction and purification and nucleic acid amplification reaction; the upper computer is in communication connection with the reaction modules, the upper computer is used for controlling the reaction modules to work independently, the upper computer comprises a display screen, and the display screen is used for displaying working state information of the reaction modules.

Description

TECHNICAL FIELD

[0001] The present disclosure relates to the technical field of biochemical analysis, and in particular to a multi-unit modular full-automatic nucleic acid amplification analysis system. BACKGROUND

[0002] At present, most of the nucleic acid detection needs to be carried out in a professional PCR laboratory, which requires very high laboratory environmental conditions and a professional fluorescence quantitative PCR instrument, and the operator also needs to have professional qualifications. This situation is that although there are many reagent manufacturers, but the institutions that can actually detect are limited. In the first line of contact with the persons to be detected, such as entry ports, highway intersections, etc., it is not possible to realize on-site detection, and only after a certain number of samples are accumulated, they can be sent to the designated detection institutions. The resulting distortion of results, time waste, and cost increase are very serious. Therefore, the demand and application of nucleic acid detection schemes that are simple to operate, fast in reaction, and suitable for various application sites and meet the POCT (point of care test) concept are increasingly widespread.

[0003] The existing PCR reaction equipment mainly has the problems of slow processing speed and high cost. If multiple samples need to be processed at the same time, multiple devices must work at the same time, which increases the use cost. CONTENT OF THE INVENTION

[0004] Therefore, it is necessary to propose a multi-unit modular full-automatic nucleic acid amplification analysis system in view of the problem of on-site detection efficiency of the existing detection equipment.

[0005] According to one aspect of the present disclosure, a multi-unit modular full-automatic nucleic acid amplification analysis system comprises: a base, the top of the base is provided with a containing cavity, a plurality of installation stations are formed in the containing cavity, each installation station is removably installed with an installation reaction module, an electrical interface is arranged on the inner wall of the containing cavity corresponding to each installation station, the electrical interface is used for electrical connection with the corresponding reaction module, and the base is further provided with a power supply interface or a power supply module connected with the electrical interface; a plurality of reaction modules, the reaction modules are used for nucleic acid extraction and purification and nucleic acid amplification reaction; and a host computer, the host computer is in communication connection with the reaction modules, the host computer is used for controlling each reaction module to work independently, and the host computer comprises a display screen, and the display screen is used for displaying the working state information of the reaction modules.

[0006] In some embodiments, along the first horizontal direction, the plurality of installation stations are linearly arranged and sequentially communicated.

[0007] In some embodiments, in the first horizontal direction, the containing cavity is open at opposite ends.

[0008] In some embodiments, the base is provided with a communication interface on the end surface in the second horizontal direction, the communication interface is in communication connection with each reaction module, the communication interface is in communication connection with the host computer through a data line, and the second horizontal direction is perpendicular to the first horizontal direction.

[0009] In some embodiments, the base is further provided with a code scanning module on the end surface in the second horizontal direction, the code scanning module is in communication connection with the communication interface, and the code scanning module is used for identifying the identity on the reaction card.

[0010] In some embodiments, each electrical interface is provided with a limiting plate on both sides.

[0011] In some embodiments, each electrical interface is provided with an elastic buckle above.

[0012] In some embodiments, the electrical interface includes a plurality of pins.

[0013] In some embodiments, the number of installation stations is less than 5.

[0014] In some embodiments, the host computer is a smart phone, a tablet computer or an industrial computer.

[0015] In the multi-unit modular full-automatic nucleic acid amplification analysis system of the utility model, a plurality of reaction modules are arranged in the base, each reaction module can perform nucleic acid extraction and purification and nucleic acid amplification reaction, and a host computer can be used to realize independent control of the plurality of reaction modules. The plurality of reaction modules can perform detection simultaneously, so that the on-site detection efficiency is improved. Compared with the traditional PCR instrument, the multi-unit modular full-automatic nucleic acid amplification analysis system of the utility model can select the number of reaction modules according to different flux requirements, the overall operation process is more simple and fast than a plurality of single-channel devices, the reaction results can be managed in batches, and the total cost is much lower than that of the same number of single-channel devices. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is a structure schematic view of the multi-unit modular full-automatic nucleic acid amplification analysis system of the utility model, wherein the base contains one reaction module.

[0017] Figure 2 It is a structure schematic view of the multi-unit modular full-automatic nucleic acid amplification analysis system of the utility model, wherein the base contains three reaction modules.

[0018] Figure 3 It is a multi-unit modular full-automatic nucleic acid amplification analysis system, wherein the upper cover of each reaction module is in an open state.

[0019] Reference signs:

[0020] 100, multi-unit modular full-automatic nucleic acid amplification analysis system; 10, base; 110, containing cavity; 120, installation station; 121, electrical interface; 122, limiting plate; 123, elastic buckle; 130, communication interface; 140, code scanning module; 20, reaction module; 210, shell; 211, upper cover; 30, upper computer; 310, display screen; 320, data line. DETAILED DESCRIPTION

[0021] To make the above objectives, features and advantages of the present disclosure more obvious and easy to understand, the specific embodiments of the present disclosure are described in detail below. In the following description, a lot of specific details are set forth in order to fully understand the present disclosure. However, the present disclosure can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the present disclosure, so the present disclosure is not limited by the specific embodiments disclosed below.

[0022] In the description of the present disclosure, it should be understood that if these terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like appear, these terms indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the purpose of facilitating the description of the present disclosure and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present disclosure.

[0023] In addition, if these terms "first", "second" appear, these terms are only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features limited by "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present disclosure, if the term "multiple" appears, the meaning of "multiple" is at least two, for example, two, three, etc., unless otherwise specifically limited.

[0024] As Figures 1 to 3 shown, wherein Figure 1 is a structural schematic diagram of the multi-unit modular full-automatic nucleic acid amplification analysis system 100 of the present utility model, wherein the base 10 contains a reaction module 20; Figure 2 is a structural schematic diagram of the multi-unit modular full-automatic nucleic acid amplification analysis system 100 of the present utility model, wherein the base 10 contains three reaction modules 20; Figure 3is a multi-unit modular full-automatic nucleic acid amplification analysis system 100, wherein the upper cover 211 of each reaction module 20 is in an open state.

[0025] The multi-unit modular full-automatic nucleic acid amplification analysis system 100 of the utility model includes a base 10, a plurality of reaction modules 20 and a host computer 30. The plurality of reaction modules 20 are removably installed in the base 10, and the host computer 30 is used for controlling each reaction module 20 to work independently.

[0026] The top of the base 10 is provided with a containing cavity 110, a plurality of installation stations 120 are formed in the containing cavity 110, and each installation station 120 removably installs one reaction module 20. The cavity inner wall of the containing cavity 110 is respectively provided with an electrical interface 121 corresponding to each installation station 120, and the electrical interface 121 is used for electrical connection with the corresponding reaction module 20, so as to deliver electric energy to each reaction module 20.

[0027] The installation station 120 is configured to be capable of placing the reaction module 20 and has a receiving space. It is easy to understand that the receiving space is part of the containing cavity 110; the size of the installation station 120 is set to be adapted to the outer dimensions of the reaction module 20.

[0028] In the utility model, the installation station 120 is provided with a plurality of. Here, the plurality refers to two or more than two. In the embodiment, as shown in Figure 1 , three installation stations 120 are specifically provided, and up to three reaction modules 20 can be placed at the same time.

[0029] Optionally, the outer surface of the base 10 is further provided with a power supply interface (not shown), which is used for connecting an external power supply. The power supply interface is electrically connected with each electrical interface 121.

[0030] Optionally, the base 10 is provided with a power supply module, and the power supply module supplies power to each reaction module 20 through the electrical interface 121. The power supply module can be at least partially embedded in the base 10. Generally, the power supply module is installed at the bottom of the base 10.

[0031] The number of reaction modules 20 is equal to the number of installation stations 120. The reaction module 20 is used for carrying out nucleic acid extraction and purification and nucleic acid amplification reaction. Optionally, the reaction module 20 includes a nucleic acid extraction unit, a nucleic acid purification unit, an amplification reaction unit and a shell 210.

[0032] The nucleic acid extraction unit is used to lyse a sample to obtain a mixture of nucleic acid and protein. The nucleic acid purification unit is used to separate the nucleic acid from the mixture. The nucleic acid amplification reaction unit is used to amplify the nucleic acid at a specified temperature using an excitation light source. The nucleic acid extraction unit, the nucleic acid purification unit, and the nucleic acid amplification reaction unit are arranged on the housing 210. The housing 210 has an openable upper cover 211, and the upper cover 211 can be opened to place a reaction card. When the upper cover 211 is closed, the host computer 30 can control the reaction module 20 to start working. Each reaction module 20 is respectively provided with a lower control module, which is used to receive the instruction of the host computer 30 and control the corresponding reaction module 20 to work according to the instruction.

[0033] It should be pointed out that in the utility model, each reaction module 20 integrates the nucleic acid extraction unit, the nucleic acid purification unit, and the amplification reaction unit, and the specific constituting mode of the nucleic acid extraction unit, the nucleic acid purification unit, and the amplification reaction unit is not limited, and any suitable constituting mode in the prior art can be adopted. The specific constitution of these units is not the focus of the present application.

[0034] The host computer 30 is in communication connection with the reaction module 20, and the host computer 30 is used to control each reaction module 20 to work independently. The host computer 30 includes a display screen 310, which is used to display the working state information of the reaction module 20. It is easy to understand that when a plurality of reaction modules 20 are installed in the base 10, the control program preset in the host computer 30 can simultaneously control the plurality of reaction modules 20 to work independently. The host computer 30 can also export the results, such as directly connecting a printer, exporting a picture file, etc.

[0035] The host computer 30 is in communication connection with the reaction module 20, and the specific mode is not limited. Optionally, the host computer 30 is in communication connection with the reaction module 20 through a wireless network. Optionally, the host computer 30 is in communication connection with the reaction module 20 through a wired network. For example, the outer surface of the base 10 is provided with a communication interface 130, and the host computer 30 is connected through the communication interface 130 through a data line 320. When the reaction module 20 is arranged in the installation station 120, the communication interface 130 is in contact with the communication pole piece of the reaction module 20 to enable communication connection.

[0036] The host computer 30 can control each reaction module 20 to work independently. Here, the host computer 30 can control each reaction module 20 to work independently, and the working of each reaction module 20 is independent of each other. In this way, the user can select one or more reaction modules 20 to work according to the needs. In other words, the multi-unit modular full-automatic nucleic acid amplification analysis system 100 in the utility model can control multiple reaction modules 20 to work at the same time to realize multi-channel detection, or can only control one reaction module 20 to work to realize single-channel detection.

[0037] The display screen 310 can control the different experimental processes of the reaction modules 20 connected to the base 10, display the reaction results, and the like. Optionally, the display screen 310 is a touch screen, so that the operation can be controlled on the display screen 310 to control the operation of the reaction modules 20.

[0038] In the multi-unit modular full-automatic nucleic acid amplification analysis system 100, a plurality of reaction modules 20 are arranged in the base 10, each reaction module 20 can perform nucleic acid extraction and purification and nucleic acid amplification reaction, and one host computer 30 can be used to independently control the plurality of reaction modules 20. The plurality of reaction modules 20 can simultaneously perform detection, thereby improving the efficiency of on-site detection. Compared with the traditional PCR instrument, the multi-unit modular full-automatic nucleic acid amplification analysis system 100 can select the number of reaction modules 20 according to different flux requirements, the overall operation process is more simple and fast than a plurality of single-channel devices, the reaction results can be batch managed, and the total cost is much lower than that of the same number of single-channel devices.

[0039] In some embodiments, along the first horizontal direction X, the plurality of installation workstations 120 are arranged in a straight line and are sequentially connected. As shown in FIG. 1B, the first horizontal direction X can be the length direction of the base 10. The plurality of installation workstations 120 are arranged in a straight line and are sequentially connected, that is, no partition is arranged in the accommodation cavity 110. In this way, when the reaction modules 20 are placed, each reaction module 20 has a large operation space. Figure 1

[0040] In other alternative embodiments, the plurality of installation workstations 120 can also not be arranged in a straight line as a whole, for example, taking three installation workstations 120 as an example, the three installation workstations 120 can be arranged in an L shape.

[0041] In some embodiments, along the first horizontal direction X, the opposite ends of the accommodation cavity 110 are open. That is, the accommodation cavity 110 penetrates the shell 210 along the first horizontal direction X. Taking three installation workstations 120 as an example, whether it is the middle reaction module 20 or the reaction modules 20 on both sides, the operation space along the first horizontal direction X is large.

[0042] ​In some embodiments, the base 10 is provided with a communication interface 130 on the end surface in the second horizontal direction Y, the communication interface 130 is in communication connection with each reaction module 20, and the communication interface 130 is in communication connection with the host computer 30 through a data line 320, the second horizontal direction Y is perpendicular to the first horizontal direction X. In this way, the communication interface 130 can transmit the control instructions of the host computer 30 to each reaction module 20, and transmit the working state information of each reaction module 20 back to the host computer 30. The second horizontal direction Y can be the width direction of the base 10. The communication interface 130 is any existing technology interface that can realize one-to-many communication connection, and the specific type is not limited.

[0043] In some embodiments, the base 10 is further provided with a code scanning module 140 on the end surface in the second horizontal direction Y, the code scanning module 140 is in communication connection with the communication interface 130, and the code scanning module 140 is used to identify the identity on the reaction card. The code scanning module 140 is shared by each reaction module 20, so as to send the identity information of the reaction card to the host computer 30, so as to determine the program to be called for the reaction. The reaction card is a microfluidic chip for nucleic acid detection. The identity is, for example, a two-dimensional code or a bar code.

[0044] When a plurality of reaction modules 20 are installed, the display screen 310 can display options corresponding to the modules (for example, options 1, 2 and 3 on the display screen 310), and the code scanning module 140 can select the corresponding reaction module 20 for experiment according to the actual needs after scanning the code on the reaction card. In this way, the host computer 30 can record the use information of the reaction card and the reaction module 20, which is convenient for later analysis and statistics. Figure 2

[0045] In some embodiments, each electrical interface 121 is provided with a limiting plate 122 on both sides. The limiting channel is formed between the two limiting plates 122, and the limiting channel is used to cooperate with the protruding block (not shown) on the reaction module 20, so as to position the reaction module 20 in the first horizontal direction X.

[0046] Further, in some embodiments, an elastic buckle 123 is arranged above each electrical interface 121. The elastic buckle 123 is used to cooperate with the card slot on the reaction module 20, so as to position the reaction module 20 in the height direction of the base 10.

[0047] In the above manner, in the first horizontal direction X, the limiting channel is used to limit the reaction module 20; in the second horizontal direction Y, the two cavity inner walls of the accommodating cavity 110 are used to limit the reaction module 20; and in the height direction of the base 10, the elastic buckle 123 is used to limit the reaction module 20. In this way, the position of the reaction module 20 is relatively stable and reliable after installation.

[0048] ​In some embodiments, the electrical interface 121 comprises a plurality of pins. The pins are matched with pinholes arranged on the reaction module 20 in a corresponding manner, and the plug-in connection facilitates the stable electrical connection after the reaction module 20 and the electrical interface 121 are docked.

[0049] In some embodiments, the number of installation stations 120 is less than or equal to 5. The installation stations 120 are arranged in a number less than or equal to 5, which can ensure that the overall system does not have an excessively large size, facilitating carrying and placement, while also achieving multi-channel on-site detection.

[0050] In some embodiments, the host computer 30 is a smartphone, a tablet computer, or an industrial computer. The host computer 30 is pre-installed with a control program for controlling the reaction module 20.

[0051] In the present disclosure, unless specifically defined otherwise and limited, if the terms "mount", "connected", "connection", "fixed", and the like are used, these terms are to be interpreted broadly. For example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise specifically limited. For those skilled in the art, the specific meaning of the above terms in the present disclosure can be understood according to the specific circumstances.

[0052] In the present disclosure, unless specifically defined otherwise and limited, if there is a description such as "on" or "under" and the like of the first feature to the second feature, it can mean that the first and second features are in direct contact, or the first and second features are indirectly in contact through an intermediate medium. Moreover, the first feature "above", "above" and "above" of the second feature can be that the first feature is directly above or obliquely above the second feature, or only indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature "below", "below" and "below" of the second feature can be that the first feature is directly below or obliquely below the second feature, or only indicates that the horizontal height of the first feature is less than that of the second feature.

[0053] It should be noted that if an element is referred to as "fixed to" or "arranged on" another element, it can be directly on the other element or there can be a middle element. If an element is considered to be "connected" to another element, it can be directly connected to the other element or there can be a middle element. If present, the terms "vertical", "horizontal", "up", "down", "left", "right", and similar expressions used in the present disclosure are only for illustrative purposes, and do not represent the only implementation.

[0054] Any technical features in the above embodiments can be combined, and for the sake of brevity, not all possible combinations are described above, however, as long as the combinations of technical features do not have contradictions, they shall be considered within the scope of the present disclosure.

[0055] The above embodiments only express several implementation manners of the present disclosure, and the description is relatively specific and detailed, but it shall not be understood as a limitation on the disclosed patent scope. It should be noted that, for those skilled in the art, several modifications and improvements can be made without departing from the concept of the present disclosure, and these shall be within the protection scope of the present disclosure. Therefore, the protection scope of the present disclosure patent shall be subject to the appended claims.

Claims

1. A multi-cell modular fully automated nucleic acid amplification analysis system, characterized by, The utility model relates to a nucleic acid extraction and amplification system, comprising: a base, the top of the base is provided with a containing cavity, a plurality of installation stations are formed in the containing cavity, each installation station is removably installed with an installation reaction module, the cavity wall of the containing cavity is respectively provided with an electrical interface corresponding to each installation station, the electrical interface is used for electrical connection with the corresponding reaction module, the base is further provided with a power supply interface or a power supply module connected with the electrical interface; a plurality of reaction modules for nucleic acid extraction and purification and nucleic acid amplification reaction; and a host computer, the host computer is in communication connection with the reaction module, the host computer is used for controlling each reaction module to work independently, the host computer includes a display screen, and the display screen is used to display the working state information of the reaction module.

2. The multi-cell modular, fully automated nucleic acid amplification analysis system of claim 1, wherein, In the first horizontal direction, a plurality of installation stations are arranged in a straight line and are sequentially communicated.

3. The multi-cell modular, fully automated, nucleic acid amplification analysis system of claim 2, wherein, In the first horizontal direction, the opposite ends of the containing cavity are open.

4. The multi-cell modular, fully automated, nucleic acid amplification analysis system of claim 2, wherein, The base is provided with a communication interface on the end face in the second horizontal direction, the communication interface is in communication connection with each reaction module, the communication interface is in communication connection with the host computer through a data line, and the second horizontal direction is perpendicular to the first horizontal direction.

5. The multi-cell modular, fully automated, nucleic acid amplification analysis system of claim 4, wherein, The base is further provided with a code scanning module on the end face in the second horizontal direction, the code scanning module is in communication connection with the communication interface, and the code scanning module is used for identifying the identity on the reaction card.

6. The multi-cell modular, fully automated, nucleic acid amplification analysis system of claim 1, wherein, Each electrical interface is respectively provided with a limiting plate on both sides.

7. The multi-cell modular, fully automated, nucleic acid amplification analysis system of claim 1, wherein, Each electrical interface is provided with an elastic buckle above.

8. The multi-cell modular, fully automated, nucleic acid amplification analysis system of claim 1, wherein, The electrical interface includes a plurality of pins.

9. The multi-cell modular, fully automated, nucleic acid amplification analysis system of claim 1, wherein, The number of installation stations is less than 5.

10. The multi-cell modular, fully automated, nucleic acid amplification analysis system of claim 1, wherein, The host computer is a smart phone, a tablet computer or an industrial computer.

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