Cell suspension transfer flask apparatus
By designing a cell suspension transfer bottle device, the direct transfer of hiHep cell suspension was realized, solving the problems of contamination risk and cumbersome operation in the existing technology, and improving the convenience of operation and aseptic safety.
Patent Information
- Application Number
- CN202423222948.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-25
- Publication Date
- 2025-11-28
- Estimated Expiration
- 2034-12-25
AI Technical Summary
In existing technologies, the transfer process of hiHep cell suspension requires removing and returning disposable transport bags from the Grade A area, which increases the risk of contamination and is cumbersome.
A cell suspension transfer bottle device was designed, including a storage bottle, an output section and a pressurization section. The cell suspension is directly transferred to the HEXA1 cell filling device by air pressure, avoiding secondary transfer steps. The entire operation is completed in the Class A area.
It reduces the risk of contamination during hiHep cell suspension transfer, improves the convenience and aseptic safety of the operation, and shortens the operation time.
Smart Images
Figure CN223605829U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to cell filling technology field especially cell suspension transfer bottle device. BACKGROUND
[0002] With the vigorous development of cell therapy and gene therapy fields in the biological medicine industry, the industry maintains a high level of sterility. Combined with the present stage biological medicine industry equipment selection, a large range selects to rely on the biosafety cabinet to maintain the local A-grade air supply environment. Among them, HepaCure plasma biological purification column needs hiHep to be fixed in a sterile bottle after completing the resuscitation and washing steps, and then transferred.
[0003] At present, there is no special hiHep cell suspension transfer device on the market. In the early stage, the cell suspension is transferred from a 1L sterile bottle to a disposable transfer bag, and finally transferred to a HEXA1 cell filler and other conventional operation steps. The last step of transfer needs to be completed under the action of gravity. The disposable transfer bag needs to be taken out from the A-grade area and put back after filling, which increases the risk of contamination. UTILITY MODEL CONTENTS
[0004] The utility model aims at providing a cell suspension transfer bottle device, which can avoid the problem of taking out the disposable transfer bag from the A-grade area and putting it back after filling, which increases the risk of contamination.
[0005] The utility model provides a cell suspension transfer bottle device, which comprises:
[0006] A liquid storage bottle body, an output part is arranged on one side of the upper end of the liquid storage bottle body, and a pressurizing part is arranged on the other side of the upper end of the liquid storage bottle body.
[0007] The output part comprises a first silica gel hose for connecting the liquid storage bottle body, a PVC pipe is connected to the upper end of the first silica gel hose, and an output connector is arranged at the upper end of the PVC pipe.
[0008] Preferably, the connection between the PVC pipe and the first silica gel hose is connected through a reducing joint, and the PVC pipes and the first silica gel hoses at both ends are respectively sleeved with the reducing joint and then fastened with a cable tie.
[0009] Preferably, a Y-shaped clamping piece can be installed at the upper end of the first silica gel hose.
[0010] Preferably, a sterile screw cap is further arranged outside the output connector.
[0011] Preferably, the upper end of the output connector is connected with a small self-sealing bag, and the end part of the output connector is directly connected with a HEXA1 cell filler.
[0012] Preferably, the upper end of the liquid storage bottle body is provided with a bottle cap, the upper end of the bottle cap is sealingly provided with a sealing head, and the inside of the sealing head is inserted with a long tube and a short tube which are inserted into the hole at the upper end of the sealing head.
[0013] Preferably, the pressurizing part comprises a second silica gel hose for communicating the inside of the liquid storage bottle body, and the upper end of the second silica gel hose is provided with a pressure device.
[0014] Preferably, the pressure device is a double ball device.
[0015] Preferably, a filter is installed at the connection position of the pressure device and the second silica gel hose.
[0016] The cell suspension transfer bottle device directly connects the output connector with the filling interface, connects the pressurizing part, and makes the pressurizing part work, so that the cell suspension in the liquid storage bottle body directly flows into the HEXA1 cell filling device under the action of air pressure, the connection between the output connector and the HEXA1 cell filling device can be disconnected after the liquid in the liquid storage bottle body is completely discharged, the hiHep cells can be transferred in one step without the previous secondary transfer step, the sterile maintenance environment of the A level is not affected, the whole operation does not involve taking the device out of the A level area and putting it back, the connection and use of the whole device can be completely completed in the A level area, the transfer of the hiHep cells is reduced, the transfer of the hiHep cell suspension is convenient and fast, the loss of the hiHep cells in the transfer process is reduced, the sterile safety is improved, and the operation time is shortened. BRIEF DESCRIPTION OF DRAWINGS
[0017] In order to more clearly illustrate the technical scheme of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments, and it should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation to the scope, and for those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.
[0018] Figure 1 It is a schematic diagram of the overall structure of the embodiment of the present application.
[0019] Figure 2 It is a schematic diagram of the cross-sectional structure of the liquid storage bottle body of the embodiment of the present application.
[0020] Figure 3 It is a schematic diagram of the output part structure of the embodiment of the present application.
[0021] Figure 4 It is a schematic diagram of the pressurizing part structure of the embodiment of the present application.
[0022] Explanation of reference signs: 100, liquid storage bottle body; 110, bottle cap; 120, sealing head; 130, long tube; 140, short tube; 200, output part; 210, first silica gel hose; 220, PVC tube; 230, reducing joint; 240, output joint; 250, sterile screw cap; 260, Y-shaped clamp; 300, pressurizing part; 310, second silica gel hose; 320, filter; 330, pressure device. DETAILED DESCRIPTION
[0023] Hereinafter, only certain exemplary embodiments are simply described. As can be appreciated by those skilled in the art, the described embodiments can be modified in various different manners without departing from the spirit or scope of the embodiments of the present application. Therefore, the drawings and the description are considered to be exemplary in nature rather than limiting.
[0024] In the description of the embodiments of the present application, it is to be understood that the orientation or positional relationship indicated by the terms "length", "vertical", "horizontal", "top", "bottom" and the like is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the embodiments of the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the embodiments of the present application.
[0025] In addition, the terms "first", "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined with "first", "second" can explicitly or implicitly include one or more of the features. In the description of the embodiments of the present application, the meaning of "multiple" is two or more, unless otherwise specifically limited.
[0026] In the embodiments of the present application, unless otherwise specifically defined and limited, the terms "mounting", "connection", "connection", "fixing" and the like should be broadly understood, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected, or it can be communicated; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the communication or interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the embodiments of the present application can be understood according to the specific circumstances.
[0027] The disclosure below provides many different implementations or examples to implement different structures of the embodiments of the present application. In order to simplify the disclosure of the embodiments of the present application, the components and settings of specific examples are described below. Of course, they are only examples, and the purpose is not to limit the embodiments of the present application. In addition, the embodiments of the present application can repeatedly refer to numbers and / or letters in different examples, and such repetition is for the purpose of simplification and clarity, which itself does not indicate the relationship between the various embodiments and / or settings discussed.
[0028] In order to better understand the purpose, structure and function of the present application, the cell suspension transfer bottle device of the present application is further described in detail below in combination with the drawings.
[0029] As shown in Figures 1-4 The embodiments of the present application provide a cell suspension transfer bottle device, which comprises a liquid storage bottle body 100 for storing and constant volume hiHep cell suspension, an output part 200 is arranged on one side of the upper end of the liquid storage bottle body 100 for transferring the cell suspension inside the liquid storage bottle body 100, and a pressurizing part 300 is arranged on the other side of the upper end of the liquid storage bottle body 100 for pressurizing the liquid in the liquid storage bottle body 100.
[0030] The output part 200 comprises a first silica gel hose 210 for connecting with the liquid storage bottle body 100, the upper end of the first silica gel hose 210 is connected with a PVC pipe 220 for assisting the delivery of the cell suspension, and the upper end of the PVC pipe 220 is provided with an output connector 240 for connecting with an output container.
[0031] The output connector 240 is directly connected with the filling interface, after the pressurizing part 300 is connected, the pressurizing part 300 works, so that the cell suspension in the liquid storage bottle body 100 flows into the HEXA1 cell filling device under the action of gas pressure through the output connector 240, after the liquid in the liquid storage bottle body 100 is completely discharged, the connection between the output connector 240 and the HEXA1 cell filling device is disconnected, the hiHep cells can be transferred in one step, the secondary transfer step is not needed, the sterile maintenance environment of A level is not affected, the whole operation does not involve taking the device out of the A level area and putting it back, the connection and use of the whole device can be completely completed in the A level area, the transfer of the hiHep cells is reduced, and the hiHep cell suspension is convenient and fast in the transfer process.
[0032] The end of the output connector 240 can be directly connected with the HEXA1 cell filling device for liquid transfer, the output connector 240 or other types of transfer connectors can be selected according to the specific connection needs, the cell suspension is conveniently transferred, the selectivity is high, and the use is flexible.
[0033] The connection between the PVC pipe 220 and the first silica gel hose 210 is connected through the reducing joint 230. The two ends of the PVC pipe 220 and the first silica gel hose 210 are respectively sleeved with the reducing joint 230 and then fastened by using a cable tie. The two ends of the PVC pipe 220 and the first silica gel hose 210 are communicated through the reducing joint 230. The reducing joint 230 can be adapted to pipes of different sizes, is flexible in connection, and has good sealing performance.
[0034] The upper end of the first silica gel hose 210 is provided with a Y-shaped clamping piece 260 for clamping and plugging the first silica gel hose 210, so as to clamp the first silica gel hose 210 and control the liquid on both sides.
[0035] The output joint 240 is also provided with a sterile screw cap 250 for plugging the output joint 240. The output joint 240 and the sterile screw cap 250 can be a female luer joint and a female luer cap. The sterile screw cap 250 can ensure that the liquid flows without leakage, is convenient to connect, and has a wide range of adaptation.
[0036] The upper end of the liquid storage bottle 100 is provided with a bottle cap 110 for sealing the liquid storage bottle 100. The bottle cap 110 can be made of polyethylene material. The upper end of the bottle cap 110 is sealingly provided with a sealing head 120 for auxiliary sealing of the bottle cap 110. The sealing head 120 is internally provided with a long pipe 130 and a short pipe 140 for pressurization and liquid outlet. The long pipe 130 and the short pipe 140 are inserted and fitted in the hole at the upper end of the sealing head 120. The lower end of the long pipe 130 is located below the liquid surface in the liquid storage bottle 100. The lower end of the short pipe 140 is located above the liquid surface in the liquid storage bottle 100 and does not contact the liquid surface. The liquid can be discharged through the long pipe 130, and the short pipe 140 is used for air inlet and pressurization.
[0037] The pressurization part 300 includes a second silica gel hose 310 for communicating the inside of the liquid storage bottle 100. The upper end of the second silica gel hose 310 is provided with a pressure device 330 for pressurizing the inside of the liquid storage bottle 100. The pressure device 330 can be a double ball device. By pressing the double ball at different frequencies, the flow rate of the liquid can be controlled, and the pressurization operation can be facilitated.
[0038] In addition, the first silica gel hose 210 can be connected with a peristaltic pump. The peristaltic pump can directly pump the liquid in the bottle into a target container through power, and has a wide range of application.
[0039] The connection position between the pressure device 330 and the second silica gel hose 310 can be provided with a filter 320 for filtering the air pressed into the liquid storage bottle 100. The filter 320 can filter the air pressed into the liquid storage bottle 100, and has good filtering effect.
[0040] The first silica gel hose 210 and the second silica gel hose 310 are both made of medical materials, are shaped, sterilized and the like, are smooth on the outer surface, have no obvious burrs on the inner surface, are suitable for use in a clean workshop and are sterilized once.
[0041] The working principle of the cell suspension transfer bottle device is as follows: the output connector 240 is directly connected with the filling interface, the pressurizing part 300 is connected, the pressurizing part 300 works, the cell suspension in the liquid storage bottle body 100 flows into the HEXA1 cell filling device under the action of the gas pressure through the output connector 240, the connection between the output connector 240 and the HEXA1 cell filling device is disconnected after the liquid in the liquid storage bottle body 100 is completely discharged, the one-step transfer of the hiHep cell can be realized, the secondary transfer step is not needed, the A-level sterile maintaining environment is not affected, the whole operation does not involve the operation of taking the device out of the A-level area and putting it back, the connection and use of the whole device can be completely completed in the A-level area, the transfer of the hiHep cell is reduced once, the transfer of the hiHep cell suspension is convenient and fast.
[0042] It can be understood that the utility model is described through some embodiments, and those skilled in the art know that various changes or equivalent replacements can be made to these features and embodiments without departing from the spirit and scope of the utility model.In addition, these features and embodiments can be modified to adapt to specific conditions and materials under the guidance of the utility model without departing from the spirit and scope of the utility model.Therefore, the utility model is not limited by the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of the present application belong to the scope protected by the utility model.
Claims
1. A cell suspension transfer flask apparatus, comprising: Include: Liquid storage bottle, the upper end of the liquid storage bottle is provided with an output part on one side, and a pressurizing part is provided on the other side of the upper end of the liquid storage bottle; The output part includes a first silica gel hose for connecting with the liquid storage bottle, and a PVC pipe is connected to the upper end of the first silica gel hose, and an output connector is arranged at the upper end of the PVC pipe.
2. The cell suspension transfer flask device of claim 1, wherein, The connection between the PVC pipe and the first silica gel hose is connected through a reducing joint, and the PVC pipe and the first silica gel hose at both ends are respectively sleeved with the reducing joint and fastened through a cable tie.
3. The cell suspension transfer flask device of claim 2, wherein, A Y-shaped clamp is installed at the upper end of the first silica gel hose.
4. The cell suspension transfer flask device of claim 3, wherein, The outside of the output connector is also provided with a sterile screw cap.
5. The cell suspension transfer flask device of claim 4, wherein, The upper end of the output connector is connected with a small self-sealing bag, and the end of the output connector is directly connected with a HEXA1 cell filling device.
6. The cell suspension transfer flask device of claim 5, wherein, The upper end of the bottle cap is sealed and installed with a sealing head, a long tube and a short tube are inserted into the inside of the sealing head, and the long tube and the short tube are inserted into the hole inside the sealing head.
7. The cell suspension transfer flask device of claim 6, wherein, The pressurizing part includes a second silica gel hose for communicating the inside of the liquid storage bottle, and a pressure device is arranged at the upper end of the second silica gel hose.
8. The cell suspension transfer flask device of claim 7, wherein, The pressure device is a double ball device.
9. The cell suspension transfer flask device of claim 8, wherein, A filter is installed at the connection position of the pressure device and the second silica gel hose.