Pipetting mechanism and PCR (Polymerase Chain Reaction) detection equipment

Abstract

The utility model discloses a pipetting mechanism and PCR (Polymerase Chain Reaction) detection equipment, and belongs to the technical field of detection equipment. The pipetting mechanism comprises a movement module; the output end of the moving module is connected with a first Z-direction moving module, a second Z-direction moving module and an unloading module, and the output end of the first Z-direction moving module can be connected with a standby suction head in an inserted mode; the output end of the second Z-direction moving module can be connected with a standby magnetic sleeve in an inserted mode. The unloading module comprises a driving part and an unloading part connected with the driving part, and the driving part is connected to the output end of the motion module; the unloading part can abut against a suction head on the first Z-direction moving module, and the first Z-direction moving module drives the suction head to move upwards so as to unload the suction head. The unloading part can abut against the magnetic sleeve on the second Z-direction moving module, the second Z-direction moving module drives the magnetic sleeve to move upwards so as to unload the magnetic sleeve, the structure is simple, and the suction head and the magnetic sleeve can be replaced conveniently.

Description

TECHNICAL FIELD

[0001] The utility model relates to detection equipment technical field especially relates to a pipette mechanism and PCR detection equipment. BACKGROUND

[0002] PCR detection is a molecular biology technology for amplifying and expanding specific DNA fragments. The PCR detection steps include: 1. Put the sample into the protease and mix to form a lysate, 2. Put the magnetic beads into the lysate, 3. Put the magnetic beads into the washing liquid for washing (multiple times), 4. Put the magnetic beads into the elution liquid to form the test liquid, 5. Transfer the test liquid to the PCR detector for detection. In the above process, sample and test liquid transfer and multiple magnetic bead transfers are required. When the same magnetic sleeve is used for multiple magnetic bead transfers and the same suction head is used for sample and test liquid transfer, contamination will occur, affecting the accuracy of the detection result, so different suction heads and magnetic sleeves need to be replaced for transfer. In the prior art, the replacement structure of the magnetic sleeve and the suction head is complex and inconvenient to operate. SUMMARY

[0003] The utility model aims at providing a pipette mechanism and PCR detection equipment, simple structure, convenient replacement of suction head and magnetic sleeve.

[0004] To achieve this purpose, the utility model adopts the following technical scheme:

[0005] A pipette mechanism, comprising a motion module; the output end of the motion module is connected with:

[0006] A first Z-direction moving module, the output end of which can be plugged with a spare suction head;

[0007] A second Z-direction moving module, the output end of which can be plugged with a spare magnetic sleeve;

[0008] An unloading module, comprising a driving member and an unloading member connected with the driving member, the driving member being connected with the output end of the motion module;

[0009] The unloading member can abut against the suction head on the first Z-direction moving module, and the first Z-direction moving module drives the suction head to move upward to unload the suction head; the unloading member can abut against the magnetic sleeve on the second Z-direction moving module, and the second Z-direction moving module drives the magnetic sleeve to move upward to unload the magnetic sleeve.

[0010] In some possible embodiments, the suction head is provided with a first flange, which can abut against a first unloading surface at the bottom end of the unloading member; the magnetic sleeve is provided with a second flange, which can abut against a second unloading surface at the bottom end of the unloading member.

[0011] In some possible implementation manners, the unloading member is provided with a first clamping hole and a second clamping hole, when the first flange abuts against the first unloading surface, the connection part of the suction head and the first Z-direction moving module is limited in the first clamping hole, and when the second flange abuts against the second unloading surface, the connection part of the magnetic sleeve and the second Z-direction moving module is limited in the second clamping hole.

[0012] In some possible implementation manners, the unloading module further comprises a transmission assembly, the driving member is connected to an input end of the transmission assembly, and the unloading member is connected to an output end of the transmission assembly.

[0013] In some possible implementation manners, the unloading module further comprises a guide assembly, the transmission assembly comprises a first connecting rod and a second connecting rod which are hingedly connected to each other, the driving member drives the second connecting rod to rotate around a vertical center line, the first connecting rod is hingedly connected to the unloading member, and the unloading member moves along a horizontal direction through the guide assembly.

[0014] In some possible implementation manners, the unloading module further comprises a mounting plate, the guide assembly comprises a guide rail and a sliding block which is slidingly connected to the guide rail, the sliding block and the driving member are both connected to the mounting plate, the unloading member is mounted on the guide rail, and the guide rail extends along the horizontal direction, so that the unloading member moves along the horizontal direction to unload the magnetic sleeve and the suction head respectively.

[0015] In some possible implementation manners, the second unloading surface and the first unloading surface are located on two sides of the unloading member along the length direction of the guide rail respectively.

[0016] In some possible implementation manners, the second unloading surface and the first unloading surface are located on two sides of the unloading member along the width direction of the guide rail respectively.

[0017] In some possible implementation manners, the output end of the first Z-direction moving module is connected with a guide pipe, one end of the guide pipe is inserted into the suction head, and the other end of the guide pipe is connected with a power pump.

[0018] The output end of the second Z-direction moving module is connected with a Z-direction driving member and a rotating driving member, the output end of the Z-direction driving member is connected with a magnetic rod, the output end of the rotating driving member is connected with a sleeve pipe, the sleeve pipe can be inserted into the magnetic sleeve, and the magnetic rod passes through the sleeve pipe and the magnetic sleeve.

[0019] A PCR detection device comprises the pipetting mechanism according to any one of the preceding solutions.

[0020] The PCR detection device has the following beneficial effects:

[0021] The pipetting mechanism and the PCR detection equipment provided by the utility model, the magnetic sleeve and the suction head adopt the same unloading module to unload, and the structure is simplified. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 is the schematic diagram of the pipetting mechanism provided by the embodiment of the utility model, and

[0023] Figure 2 is the bottom view of the pipetting mechanism provided by the embodiment of the utility model.

[0024] In the drawings,

[0025] 200, pipetting mechanism; 210, movement module; 211, X-direction moving module; 212, Y-direction moving module; 213, support plate; 230, second Z-direction moving module; 240, first Z-direction moving module; 250, magnetic sleeve; 251, second flange; 260, suction head; 261, first flange; 270, unloading module; 271, driving piece; 272, unloading piece; 2721, second clamping opening; 2722, first clamping opening; 273, transmission assembly; 2731, second connecting rod; 2732, first connecting rod; 274, guide assembly; 2741, guide rail; 2742, sliding block; 275, mounting plate; 281, conduit; 282, power pump; 291, sleeve; 292, magnetic rod; 293, hollow brushless motor; 294, Z-direction driving piece. DETAILED DESCRIPTION

[0026] In order to make the technical problems solved by the utility model, the technical scheme adopted and the technical effects reached more clear, the technical scheme of the embodiment of the utility model will be further described in detail below in combination with the drawings. Obviously, the described embodiment is only a part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all the other embodiments obtained by the person skilled in the art without making creative labor belong to the range protected by the utility model.

[0027] In the description of the utility model, unless another definite provision and limitation, the term "connect", "connection", "fixed" should be broad sense understanding, for example, can be fixed connection, also can be detachable connection, or into an organic whole;Can be mechanical connection, also can be electrical connection;Can be directly connected, also can be indirectly connected through the intermediate medium, can be two elements inside the communication or two elements of the interaction relationship.For the ordinary skilled in the art, the above-mentioned terms can be understood in the utility model according to the specific meaning of the specific circumstances.

[0028] In the utility model, unless another definite provision and limitation, the first feature is "on" or "under" the second feature can include the first and second features direct contact, also can include the first and second features is not direct contact but is through the contact between other features of them.And, the first feature is "on", "above" and "on" the second feature includes the first feature is directly above and obliquely above the second feature, or just indicates that the first feature horizontal height is higher than the second feature.The first feature is "under", "below" and "under" the second feature includes the first feature is directly below and obliquely below the second feature, or just indicates that the first feature horizontal height is less than the second feature.

[0029] As Figure 1 And Figure 2 As shown in the embodiment, a PCR detection device is provided, which comprises a pipetting mechanism 200.The embodiment also provides a pipetting mechanism, which comprises a movement module 210 and a first Z-direction moving module 240, a second Z-direction moving module 230 and an unloading module 270 connected to the output end of the movement module 210.The output end of the first Z-direction moving module 240 can be plugged with a spare suction head 260, and the output end of the second Z-direction moving module 230 can be plugged with a spare magnetic sleeve 250.The unloading module 270 comprises a driving member 271 and an unloading member 272 connected to the driving member 271, and the driving member 271 is connected to the output end of the movement module 210.The unloading member 272 can abut against the suction head 260 on the first Z-direction moving module 240, and the first Z-direction moving module 240 drives the suction head 260 to move upward to unload the suction head 260.The unloading member 272 can abut against the magnetic sleeve 250 on the second Z-direction moving module 230, and the second Z-direction moving module 230 drives the magnetic sleeve 250 to move upward to unload the magnetic sleeve 250.

[0030] Optionally, when the PCR detection is carried out by using the above-mentioned pipetting mechanism 200, the following steps are included.

[0031] Step one, the first Z-direction moving module 240 drives the suction head 260 to move downward, the suction head 260 takes out the sample and puts it into the protease to mix and form a lysis solution; the movement module 210 drives the first Z-direction moving module 240 and the suction head 260 thereon to a preset recovery position, the driving part 271 drives the unloading part 272 to abut against the suction head 260, and then the first Z-direction moving module 240 drives the suction head 260 to move upward, the suction head 260 is taken off for recovery; the movement module 210 drives the first Z-direction moving module 240 to the standby suction head 260, and the two are connected to each other for the next time of liquid suction. Step two, the second Z-direction moving module 230 drives the magnetic sleeve 250 to move downward to put the magnetic beads into the lysis solution, the movement module 210 drives the second Z-direction moving module 230 and the magnetic sleeve 250 thereon to a preset recovery position, the driving part 271 drives the unloading part 272 to abut against the magnetic sleeve 250, and then the second Z-direction moving module 230 drives the magnetic sleeve 250 to move upward, the magnetic sleeve 250 is taken off for recovery; the movement module 210 drives the second Z-direction moving module 230 to the standby magnetic sleeve 250, and the two are connected to each other for the next time of magnetic bead taking and placing. In the following steps, the steps in step one and step two are used to unload and replace the suction head 260 and the magnetic sleeve 250: step three, the magnetic beads are put into the washing solution for washing (which can be done for multiple times). Step four, the magnetic beads are put into the elution solution to form a to-be-tested solution. Step five, the to-be-tested solution is transferred to a PCR detector for detection. In the above different steps, a new suction head 260 is used for sample and to-be-tested solution transfer to avoid cross contamination, and a new magnetic sleeve 250 is used for multiple magnetic bead transfer, also to avoid cross contamination, thereby improving the accuracy of the detection result.

[0032] The magnetic sleeve 250 and the suction head 260 are unloaded by the same unloading module 270, which simplifies the structure. Since the unloading module 270, the first Z-direction moving module 240 for connecting the suction head 260 and the second Z-direction moving module 230 for connecting the magnetic sleeve 250 are simultaneously arranged at the output end of the movement module 210, the positions of the unloading module 270, the suction head 260 and the magnetic sleeve 250 are relatively fixed, the movement precision of the movement module 210 does not affect the cooperation precision of the unloading module 270 and the suction head 260 and the magnetic sleeve 250, and the interference factors are reduced. During unloading, only the driving part 271 needs to drive the unloading part 272 to cooperate with the suction head 260 or the magnetic sleeve 250, and the suction head 260 and the magnetic sleeve 250 move upward, so that the suction head 260 or the magnetic sleeve 250 can be unloaded, the cooperation is simple, and the operation is convenient. The suction head 260 and the magnetic sleeve 250 are connected to the pipetting mechanism, which is convenient for installation and disassembly, that is, convenient for replacement.

[0033] The output end of the first Z-direction moving module 240 is connected with a conduit 281, one end of the conduit 281 is inserted into the suction head 260, and the other end of the conduit 281 is connected with a power pump 282. When the power pump 282 is started, the suction head 260 is enabled to suck or release liquid through the conduit 281, so as to realize liquid transfer. Exemplarily, the power pump 282 can be a micropump, which can be simultaneously installed with the conduit 281 at the output end of the first Z-direction moving module 240, so as to minimize the length of the conduit 281 and simplify the overall structure. Alternatively, the power pump 282 can be externally arranged, and only the conduit 281 is installed at the output end of the first Z-direction moving module 240, so as to reduce the load of the moving module 210 and the first Z-direction moving module 240.

[0034] The output end of the second Z-direction moving module 230 is connected with a Z-direction driving member 294 and a rotary driving member. The output end of the Z-direction driving member 294 is connected with a magnetic rod 292, and the output end of the rotary driving member is connected with a sleeve 291. The sleeve 291 can be inserted into the magnetic sleeve 250, and the magnetic rod 292 penetrates through the sleeve 291 and the magnetic sleeve 250. Specifically, the rotary driving member is a hollow brushless motor 293, the magnetic rod 292 penetrates through the hollow brushless motor 293, the sleeve 291 and the magnetic sleeve 250, and the Z-direction driving member 294 drives the magnetic rod 292 to move up and down. When the magnetic beads are taken out, the magnetic rod 292 moves downward to contact the magnetic beads to realize taking out, and the magnetic rod 292 moves upward to separate from the magnetic beads to realize releasing the magnetic beads. When the hollow brushless motor 293 drives the sleeve 291 and the magnetic sleeve 250 to rotate, liquid mixing can be realized.

[0035] The suction head 260 is provided with a first flange 261, which can abut against a first unloading surface at the bottom end of the unloading member 272. By arranging the first flange 261, the contact area of the suction head 260 and the unloading member 272 is increased, and the unloading reliability of the suction head 260 is improved. Exemplarily, the first flange 261 is arranged at the top of the suction head 260, which facilitates contact with the unloading member 272 and is convenient and reliable to operate. Similarly, the magnetic sleeve 250 is provided with a second flange 251, which can abut against a second unloading surface at the bottom end of the unloading member 272. By arranging the second flange 251, the contact area of the magnetic sleeve 250 and the unloading member 272 is increased, and the unloading reliability of the magnetic sleeve 250 is improved. Exemplarily, the second flange 251 is arranged at the top of the magnetic sleeve 250, which facilitates contact with the unloading member 272 and is convenient and reliable to operate.

[0036] The first flange 261 is abutted on the first unloading surface, the connection part of the suction head 260 and the first Z-direction moving module 240 is limited in the first clamping hole 2722, specifically, the catheter 281 is limited in the first clamping hole 2722, so that the suction head 260 is prevented from being separated from the first unloading surface due to shaking during upward movement of the first Z-direction moving module 240, and the connection reliability and tightness of the two are ensured, thereby improving the unloading reliability.

[0037] The second flange 251 is abutted on the second unloading surface, the connection part of the magnetic sleeve 250 and the second Z-direction moving module 230 is limited in the second clamping hole 2721, specifically, the sleeve 291 is limited in the second clamping hole 2721, so that the magnetic sleeve 250 is prevented from being separated from the second unloading surface due to shaking during upward movement of the second Z-direction moving module 230, and the connection reliability and tightness of the two are ensured, thereby improving the unloading reliability.

[0038] The unloading module 270 further comprises a transmission assembly 273, the driving member 271 is drivingly connected to an input end of the transmission assembly 273, and the unloading member 272 is connected to an output end of the transmission assembly 273, so that the unloading member 272 can be more stably matched with the suction head 260 or the magnetic sleeve 250 through the transmission assembly 273, and impact is prevented from occurring to affect the structural stability.

[0039] The unloading module 270 further comprises a guide assembly 274, the transmission assembly 273 comprises a first connecting rod 2732 and a second connecting rod 2731 which are hingedly connected to each other, the driving member 271 drives the second connecting rod 2731 to rotate about a vertical center line, the first connecting rod 2732 is hingedly connected to the unloading member 272, and the unloading member 272 moves along a horizontal direction through the guide assembly 274, so that the structure is simple and the movement precision of the unloading member 272 is ensured, and the unloading member 272 is accurately matched with the suction head 260 and the magnetic sleeve 250, respectively. Exemplarily, the unloading module 270 further comprises a mounting plate 275, the guide assembly 274 comprises a guide rail 2741 and a sliding block 2742 which is slidingly connected to the guide rail 2741, the sliding block 2742 and the driving member 271 are both connected to the mounting plate 275, and the unloading member 272 is mounted on the guide rail 2741, the guide rail 2741 extends along the horizontal direction, so that the unloading member 272 moves along the horizontal direction to unload the magnetic sleeve 250 and the suction head 260, respectively.

[0040] The second unloading surface and the first unloading surface are respectively located at two sides of the unloading piece 272 along the length direction of the guide rail 2741, that is, the first clamping opening 2722 and the second clamping opening 2721 are located at two sides of the unloading piece 272 along the length direction of the guide rail 2741, the unloading piece 272 is located at the middle position of the suction head 260 and the magnetic sleeve 250, and the moving distance of the unloading piece 272 when cooperating with the magnetic sleeve 250 and the suction head 260 respectively is reduced. The second unloading surface is the edge position of the second clamping opening 2721, and the first unloading surface is the edge position of the first clamping opening 2722. The second unloading surface and the first unloading surface are respectively located at two sides of the unloading piece 272 along the width direction of the guide rail 2741, that is, the first clamping opening 2722 and the second clamping opening 2721 are located at two sides of the unloading piece 272 along the width direction of the guide rail 2741, and structural interference is prevented.

[0041] Optionally, the motion module 210 comprises an X-direction moving module 211 and a Y-direction moving module 212, the Y-direction moving module 212 is connected to the output end of the X-direction moving module 211, the output end of the Y-direction moving module 212 is connected with a support plate 213, and the first Z-direction moving module 240, the second Z-direction moving module 230 and the unloading module 270 are all connected to the support plate 213. The X-direction moving module 211, the Y-direction moving module 212, the first Z-direction moving module 240 and the second Z-direction moving module 230 can all adopt a linear module or a motor plus a screw nut pair structure in the prior art, and will not be described in detail. The driving piece 271 can be a motor, and the output shaft of the motor drives the second connecting rod 2731 to rotate.

[0042] Obviously, the above embodiments of the present application are merely exemplary and are not intended to limit the embodiments of the present application. For those skilled in the art, other different forms of changes or modifications can be made on the basis of the above description. Here, it is not necessary and impossible to enumerate all the embodiments. Any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the claims of the present application.

Claims

1. A pipetting mechanism, characterized in that, The motion module (210) is connected with A first Z-direction moving module (240) is connected with a spare suction head (260); A second Z-direction moving module (230) is connected with a spare magnetic sleeve (250); An unloading module (270) is connected with the output end of the motion module (210), and includes a driving member (271) and an unloading member (272) connected with the driving member (271). The unloading member (272) is capable of abutting against the suction head (260) on the first Z-direction moving module (240), and the first Z-direction moving module (240) drives the suction head (260) to move upward to unload the suction head (260); the unloading member (272) is capable of abutting against the magnetic sleeve (250) on the second Z-direction moving module (230), and the second Z-direction moving module (230) drives the magnetic sleeve (250) to move upward to unload the magnetic sleeve (250).

2. The pipetting mechanism of claim 1, wherein, The suction head (260) is provided with a first flange (261) capable of abutting against a first unloading surface at the bottom end of the unloading member (272); the magnetic sleeve (250) is provided with a second flange (251) capable of abutting against a second unloading surface at the bottom end of the unloading member (272).

3. The pipetting mechanism of claim 2, wherein, The unloading member (272) is provided with a first clamping hole (2722) and a second clamping hole (2721), when the first flange (261) abuts against the first unloading surface, the connection between the suction head (260) and the first Z-direction moving module (240) is limited in the first clamping hole (2722); when the second flange (251) abuts against the second unloading surface, the connection between the magnetic sleeve (250) and the second Z-direction moving module (230) is limited in the second clamping hole (2721).

4. The pipetting mechanism of claim 2, wherein, The unloading module (270) further includes a transmission assembly (273), the driving member (271) drives the input end of the transmission assembly (273), and the unloading member (272) is connected with the output end of the transmission assembly (273).

5. The pipetting mechanism of claim 4, wherein, The unloading module (270) further includes a guide assembly (274), the transmission assembly (273) includes a first connecting rod (2732) and a second connecting rod (2731) hingedly connected with each other, the driving member (271) drives the second connecting rod (2731) to rotate around a vertical center line, the first connecting rod (2732) is hingedly connected with the unloading member (272), and the unloading member (272) moves along a horizontal direction through the guide assembly (274).

6. The pipetting mechanism of claim 5, wherein, The unloading module (270) further comprises a mounting plate (275), the guide assembly (274) comprises a guide rail (2741) and a sliding block (2742) slidably connected to the guide rail (2741), the sliding block (2742) and the driving member (271) are both connected to the mounting plate (275), the unloading member (272) is mounted to the guide rail (2741), the guide rail (2741) extends along the horizontal direction, so that the unloading member (272) moves along the horizontal direction to unload the magnetic sleeve (250) and the suction head (260) respectively.

7. The pipetting mechanism of claim 6, wherein, The second unloading surface and the first unloading surface are located on two sides of the unloading member (272) along the length direction of the guide rail (2741) respectively.

8. The pipetting mechanism of claim 6, wherein, The second unloading surface and the first unloading surface are located on two sides of the unloading member (272) along the width direction of the guide rail (2741) respectively.

9. The pipetting mechanism according to any one of claims 1 to 8, characterized in that The output end of the first Z-direction moving module (240) is connected with a catheter (281), one end of the catheter (281) is inserted into the suction head (260), and the other end of the catheter (281) is connected with a power pump (282). The output end of the second Z-direction moving module (230) is connected with a Z-direction driving member (294) and a rotary driving member, the output end of the Z-direction driving member (294) is connected with a magnetic rod (292), the output end of the rotary driving member is connected with a sleeve (291), the sleeve (291) can be inserted into the magnetic sleeve (250), and the magnetic rod (292) penetrates through the sleeve (291) and the magnetic sleeve (250).

10. A PCR detection device, characterized by, A pipetting mechanism as claimed in any of claims 1 to 9.

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