Measuring device of microplate reader

Abstract

The utility model discloses a microplate reader measuring device, which belongs to the technical field of microplate reader measuring and comprises a base, a light source component and a light filtering device are arranged on the base, the light source component is slidably arranged on the base and comprises a mounting seat and a light source, a hollowed-out groove is arranged in the mounting seat, and the light source is arranged at the position corresponding to the hollowed-out groove. And a first light through hole aligned with the light filtering device is formed in the hollow groove. According to the scheme, the light source assembly can move relative to the base, so that the distance between the light source assembly and the light filtering device is adjusted, the most appropriate position can be selected to enable the light source to reach the strongest light source, the light source switching speed can be rapidly increased, the strongest light source can be debugged, and the accuracy and timeliness of experimental data results are greatly improved; in addition, the mounting base has a fixing effect on the light source, the hollow-out groove in the mounting base can improve the intensity of light rays generated by the light source, then the light rays are emitted out through the first light through hole, and the light effect is greatly improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to a biological sample detection device more specifically, it relates to an enzyme label appearance measuring device BACKGROUND

[0002] In the medical field, for the determination and analysis of certain molecules in biological samples, it is often necessary to place them in a specific enzyme label appearance for detection. Therefore, a higher precision and better range enzyme label appearance becomes a widespread and necessary existence in the medical field. In the automatic medical instrument operation experiment process, enzyme label appearance measurement is inevitable and frequent, so the detection path and precision of light absorption become an important function of the enzyme label appearance. Among the enzyme label appearance on the market, the absorbance is generally low in efficiency, which reduces the accuracy of experimental data results.

[0003] For example: Chinese patent publication No. CN202562843U, publication date November 28, 2012, the name of the utility model is enzyme label appearance suitable for open environment, including along the light path in order distribution of complex light source, first lens group, optical filter unit, optical fiber light transmission beam, second lens group and light beam receiving detection unit, the second lens group and light beam receiving detection unit between the enzyme label plate to be detected are placed, also includes the optical modulation disc between the complex light source and the first lens group, the optical modulation disc is driven by a motor and rotates around its own axis, a plurality of light transmission holes for light beam to pass through are uniformly distributed along the circumference of the optical modulation disc. The utility model can inhibit the interference caused by light source fluctuation and background light, and at the same time, the signal is subjected to spatial filtering, noise suppression and external electromagnetic wave interference. This scheme can work in an open environment, has higher sensitivity and smaller error. However, this scheme cannot adjust the light source, and the light source effect is not good. During the measurement process, only a small range and range are available, and the reliability of the measurement results needs to be improved. UTILITY MODEL CONTENTS

[0004] The utility model overcomes the problem that the light source of the existing enzyme label appearance measuring device cannot be adjusted and the light effect is not good, provides an enzyme label appearance measuring device, the position of the light source can be adjusted in this scheme, the light effect is stable, the experimental efficiency is greatly improved; The experimental results of high precision and larger range also make the experimental results more reliable.

[0005] In order to solve the above technical problems, the utility model discloses the following technical scheme: A microplate metering device, including base, be equipped with light source assembly and filter device on the base, the light source assembly can be slidably arranged in the base, the light source assembly includes the mounting seat and light source, the mounting seat is equipped with the hollow slot inside, is equipped with the light source corresponding the hollow slot position, is equipped with the first light hole that aligns with the filter device in the hollow slot. In the scheme, the light source assembly can move relative to the base, thereby adjusting the distance between the light source assembly and the filter device, can select the most suitable position and make the light source reach the strongest, can also quickly improve the speed of switching the light source and debug the strongest light source, greatly improve the accuracy and timeliness of experimental data results, in addition, the mounting seat plays the effect of fixing to the light source, the hollow slot in the mounting seat can improve the light intensity generated by the light source, and then the first light hole is shot, and the light effect is greatly improved. The experimental efficiency and accuracy are improved.

[0006] As preferred, the light source comprises a light source mounting plate, the light source mounting plate is provided with a lamp body and a heat sink, and the light source mounting plate is arranged between the mounting seat and the heat sink. The light source mounting plate is a thin plate structure and is arranged between the hollow groove of the mounting seat and the heat sink, so that the light production effect of the lamp body is not hindered, and the heat sink can also play a heat dissipation effect on the lamp body.

[0007] As preferred, the filter device comprises a rotating disc, a plurality of filter holes are uniformly distributed in a ring shape on the rotating disc, and a filter plate is arranged in each filter hole. The rotating disc can be rotated to switch different filter holes, and different filter plates are used to filter light.

[0008] As preferred, the filter hole and the lamp body are located on the same axis. The filter hole and the lamp body are located on the same axis, so that the light generated by the lamp body can enter the filter hole as much as possible, and then be filtered by the filter plate.

[0009] As preferred, the filter device further comprises a sensor located outside the rotating disc in a ring shape, and the outer ring of the rotating disc is further provided with a zero position hole. The sensor is a photoelectric switch, and the sensor takes the zero position hole on the outer periphery of the rotating disc as a reference, so as to control the angle of rotation of the rotating disc and control the alignment of different filter holes with the lamp body.

[0010] As preferred, the filter plate is arranged in the filter hole close to the zero position hole of the rotating disc. The filter plate is preferentially arranged in the filter hole close to the zero position hole, so as to reduce the angle of rotation of the rotating disc and improve the measurement efficiency. At the same time, the rotating disc can be bidirectionally rotated, so as to reduce the rotation time.

[0011] As preferred, a spectrum emission tube seat is further included, and a fiber bundle is arranged between the light filtering device and the spectrum emission tube seat, a second light passing hole is arranged on the spectrum emission tube seat, and a plano-convex mirror is arranged in the second light passing hole.

[0012] As preferred, a spectrum absorption tube seat is further included, a third light passing hole is arranged on the spectrum absorption tube seat, and a lenticular mirror is arranged in the third light passing hole. After passing through the second light passing hole, the light passes through the biological sample and enters the third light passing hole, and then is focused by the lenticular mirror, and finally generates an analog quantity by the silicon photodiode to obtain an experimental result.

[0013] As preferred, the fiber bundle is an eight-way fiber bundle. The fiber bundle is an eight-way fiber bundle, so that the light passing through the light filtering device can be evenly distributed to eight different channels, so that multiple samples can be detected at the same time, and the detection efficiency is greatly improved.

[0014] Compared with the prior art, the present application has the following beneficial effects: (1) the light source can be controlled, the light effect can be quickly adjusted, and the light intensity can reach the strongest; (2) the four-color lamp beads are used as the light source, the high-precision experimental result and the larger range of the range also make the experimental result more reliable; (3) the eight-way fiber bundle is used, and the experimental efficiency is greatly improved. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is a front view of the present application.

[0016] Figure 2 It is Figure 1 It is a sectional view in A-A direction.

[0017] Figure 3 It is a principle flow chart of the present application.

[0018] In the figure: 1. base, 2. mounting seat, 3. light source mounting plate, 4. hollow groove, 5. first light passing hole, 6. heat sink, 7. rotating disc, 8. light filtering hole, 9. sensor, 10. zero hole, 11. spectrum emission tube seat, 12. fiber bundle, 13. second light passing hole, 14. third light passing hole, 15. spectrum absorption tube seat, 16. long waist hole, 17. driving device, 18. driving device mounting plate, 19. avoiding groove, 20. fiber mounting seat, 21. sensor mounting plate. DETAILED DESCRIPTION

[0019] The technical scheme of the present application will be further specifically described below by means of specific embodiments and in combination with the drawings.

[0020] Embodiment 1: an enzyme-labeled instrument measuring device as shown in Figures 1 to 3 The light source assembly and the light filtering device are arranged on the base 1 and are spaced apart, wherein the light filtering device is fixed on the base 1, and the light source assembly can slide relative to the base 1, so that the distance between the light source assembly and the light filtering device can be changed to adjust the light effect generated by the light source, the speed of switching the light source can be quickly improved, and the strongest light source can be debugged, thereby greatly improving the accuracy and timeliness of experimental data results.

[0021] Specifically, the light source assembly includes a mounting seat 2, a light source, a light source mounting plate 3, and a heat sink 6, etc. The mounting seat 2 is a vertically arranged plate-shaped structure, and a protruding mounting portion is arranged at the bottom of the mounting seat 2. The mounting seat 2 is connected with the base 1 through the mounting portion. A hollow groove 4 is arranged on the side of the mounting seat 2 away from the light filtering device, and a first light passing hole 5 is arranged in the hollow groove 4. The first light passing hole 5 penetrates through the surface of the mounting seat 2 on the side close to the light filtering device, and the overall size of the hollow groove 4 is larger than the size of the first light passing hole 5, so that the light generated by the light source is collected into the first light passing hole 5 and emitted. The light source mounting plate 3 and the heat sink 6 are arranged on the side of the mounting seat 2 away from the light filtering device. The light source mounting plate 3 is arranged between the mounting seat 2 and the heat sink 6, and the position of the light source mounting plate 3 covers the above-mentioned hollow groove 4. A lamp body is arranged on the light source mounting plate 3, and the lamp body is a four-color light bead close to the side of the hollow groove 4. The hollow groove 4 provides a containing space for the four-color light bead, and the light is emitted as much as possible towards the first light passing hole 5 through the covering of the light source mounting plate 3, thereby improving the light intensity. The heat sink 6 is arranged on the back surface of the light source mounting plate 3, and the heat sink 6 is arranged in multiple and perpendicular to the surface of the light source mounting plate 3, thereby providing a good heat dissipation effect for the whole light source assembly. The four-color light bead is used as the light source to improve the accuracy and flexibility of the measurement. When the four-color light bead is used as the light source, four different wavelengths of light can be emitted. These light waves become monochromatic light through the filter, a part is absorbed by the biological sample, and another part is transmitted through the sample to the photoelectric detector. Different biological molecules have different absorption characteristics for different wavelengths of light, so the use of the four-color light bead can provide more information, and the measurement result is more accurate.

[0022] Further, the mounting seat 2 and the base 1 are in sliding connection, and in an embodiment, a long waist hole 16 is arranged on the mounting portion at the bottom of the mounting seat 2, and a bolt fixing member is arranged on the corresponding base 1, the long waist hole 16 enables the mounting seat 2 to have a certain moving range, when the mounting seat 2 is moved to a suitable position, the bolt member is tightened to realize the fixing between the mounting seat 2 and the base 1; of course, the long waist hole 16 can also be arranged on the base 1, and the same moving effect can be realized, the adjustment range of this mode is limited by the length of the long waist hole 16, and the overall size of the mounting seat 2 is not suitable to be too large, so it is suitable for small-range adjustment. In another embodiment, a sliding structure of a sliding groove and a sliding block is used on the bottom of the mounting seat 2 and the upper surface of the base 1, and the same sliding effect between the mounting seat 2 and the base 1 can also be realized, and the adjustment range of this mode is limited by the length of the sliding groove, and the length of the sliding groove can be arranged on the base 1 at will, and even can penetrate the edge of the base 1, so this scheme is suitable for large-range adjustment requirements.

[0023] The light filtering device includes a rotating disc 7, a sensor 9, a driving device 17 and the like, and a driving device mounting plate 18 is arranged on the base 1, the driving device mounting plate 18 is in L shape, one end of the driving device mounting plate 18 is fixedly connected with the base 1, and the other end of the driving device mounting plate 18 is fixedly connected with the driving device 17, the driving device 17 is a rotary motor, a connecting rod is arranged on the output end of the driving device 17, and the connecting rod connects the rotating disc 7. The rotating disc 7 can be driven to rotate after the driving device 17 is started. The rotating disc 7 is arranged vertically, and the axial end surface of the rotating disc 7 is parallel to the light source mounting plate 3. An avoiding groove 19 is arranged on the base 1 and corresponds to the bottom of the rotating disc 7, and part of the bottom of the rotating disc 7 is located in the avoiding groove 19, so that the overall height of the light filtering device can be reduced to a certain extent.

[0024] Eight groups of light filtering holes 8 are arranged on the rotating disc 7, and the number of the light filtering holes 8 can be determined according to actual needs. The eight groups of light filtering holes 8 are annularly distributed on the rotating disc 7, and four groups of filters are arranged in the light filtering holes 8, and the four groups of filters are respectively BP450-8, BP492-10, BP630-10 and BP405-8. It should be noted that the light source and one of the light filtering holes 8 on the rotating disc 7 can realize axis coincidence, that is, in the rotating process, the light filtering holes 8 on the rotating disc 7 can all reach the position aligned with the light source, so that the light generated by the light source can enter the corresponding light filtering hole 8, and then be filtered by the filter.

[0025] The outer ring of the rotating disc 7 is also provided with a zero position hole 10, which is a slot hole structure arranged on the rotating disc 7, and a sensor 9 is arranged on the radial outer part of the rotating disc 7. Specifically, the base 1 is also provided with a fiber mounting seat 20, and a sensor mounting plate 21 is arranged on the fiber mounting seat 20. The fiber mounting seat 20 is arranged on the side of the rotating disc 7 away from the light source assembly, one side of the sensor mounting plate 21 is fixedly connected to the fiber mounting seat 20, and the other end extends to the side of the rotating disc 7 close to the light source assembly. The sensor 9 is an optical switch and is arranged on the side of the sensor mounting plate 21 close to the light source assembly. The sensor 9 can detect the position of the zero position hole 10 on the rotating disc 7. Taking the zero position hole 10 as the reference, the corresponding angle of rotation of the rotating disc 7 can be controlled, so that the corresponding light filtering hole 8 and the light source are aligned.

[0026] In this scheme, only four groups of filter plates are needed, so the filter plates are preferentially arranged in the light filtering hole 8 of the rotating disc 7 close to the zero position hole 10, so that the rotation angle of the rotating disc 7 can be reduced.

[0027] Embodiment 2: an enzyme marker measuring device as shown in Figures 1 to 3 The light source assembly and the light filtering device are arranged on the base 1, and the light source assembly and the light filtering device are distributed at intervals. The light filtering device is fixed on the base 1, and the light source assembly can slide relative to the base 1, so as to change the distance between the light source assembly and the light filtering device, adjust the light effect generated by the light source, quickly improve the speed of switching the light source, and debug the strongest light source, greatly improve the accuracy and timeliness of the experimental data results.

[0028] Specifically, the light source assembly includes a mounting seat 2, a light source, a light source mounting plate 3, and a heat sink 6, etc. The mounting seat 2 is a vertically arranged plate-shaped structure, and a protruding mounting portion is arranged at the bottom of the mounting seat 2. The mounting seat 2 is connected with the base 1 through the mounting portion. A hollow groove 4 is arranged on the mounting seat 2 away from the filter device side, and a first light transmission hole 5 is arranged in the hollow groove 4. The first light transmission hole 5 penetrates the surface of the mounting seat 2 close to the filter device side, and the overall size of the hollow groove 4 is larger than the size of the first light transmission hole 5, so that the light generated by the light source is collected into the first light transmission hole 5 and emitted. The light source mounting plate 3 and the heat sink 6 are arranged on the side of the mounting seat 2 away from the filter device. The light source mounting plate 3 is arranged between the mounting seat 2 and the heat sink 6, and the position of the light source mounting plate 3 covers the above-mentioned hollow groove 4. The light source mounting plate 3 is provided with a lamp body, which is a four-color light bead and is close to the side of the hollow groove 4. The hollow groove 4 provides a containing space for the four-color light bead, and the light is emitted as much as possible towards the first light transmission hole 5 through the covering of the light source mounting plate 3, thereby improving the light intensity. The heat sink 6 is arranged on the back surface of the light source mounting plate 3. The heat sink 6 is provided with a plurality of heat sinks and is arranged perpendicular to the surface of the light source mounting plate 3, which can provide good heat dissipation effect for the whole light source assembly. Among them, using four-color light beads as light source can improve the accuracy and flexibility of the determination; when using four-color light beads as light source, four different wavelengths of light can be emitted. These light waves become monochromatic light after passing through the filter, a part is absorbed by the biological sample, and another part is transmitted through the sample and irradiated on the photoelectric detector. Because different biological molecules have different absorption characteristics for different wavelengths of light, using four-color light beads can provide more information, so that the determination result is more accurate.

[0029] Further, the mounting seat 2 and the base 1 are connected by sliding. In one embodiment, a long waist hole 16 is arranged on the mounting portion at the bottom of the mounting seat 2, and a bolt fixing member is arranged on the base 1. The long waist hole 16 enables the mounting seat 2 to have a certain range of movement. When the mounting seat 2 is moved to the appropriate position, the bolt member is tightened to fix the mounting seat 2 and the base 1. Of course, the long waist hole 16 can also be arranged on the base 1 to achieve the same movement effect. The adjustment range of this mode is limited by the length of the long waist hole 16, and the overall size of the mounting seat 2 should not be too large, so it is suitable for small range adjustment. In another embodiment, a sliding structure of sliding groove and sliding block is used on the bottom of the mounting seat 2 and the upper surface of the base 1, which can also achieve the same sliding effect between the mounting seat 2 and the base 1. The adjustment range of this mode is limited by the length of the sliding groove, and the length of the sliding groove can be set arbitrarily on the base 1, even can penetrate the edge of the base 1. Therefore, this scheme is suitable for large range adjustment requirement.

[0030] The filter device comprises a rotating disc 7, a sensor 9, a driving device 17 and the like. An L-shaped driving device mounting plate 18 is arranged on the base 1. One end of the driving device mounting plate 18 is fixedly connected with the base 1, and the other end of the driving device mounting plate 18 is fixedly connected with the driving device 17. The driving device 17 is a rotary motor. A connecting rod is arranged on the output end of the driving device 17, and the connecting rod is connected with the rotating disc 7. The rotating disc 7 can be driven to rotate by the driving device 17. The rotating disc 7 is arranged vertically, and the axial end surface of the rotating disc 7 is parallel to the light source mounting plate 3. An avoiding groove 19 is arranged on the base 1 and corresponds to the bottom of the rotating disc 7. The bottom of the rotating disc 7 is located in the avoiding groove 19, so that the overall height of the filter device can be reduced to a certain extent.

[0031] Eight groups of filter holes 8 are arranged on the rotating disc 7. The number of the filter holes 8 can be determined according to actual needs. The eight groups of filter holes 8 are arranged in a ring shape on the rotating disc 7. Four groups of filters are arranged in the filter holes 8. The four groups of filters are BP450-8, BP492-10, BP630-10 and BP405-8 respectively. It should be noted that the light source and one filter hole 8 on the rotating disc 7 can realize axis coincidence. That is, in the rotating process, the filter holes 8 on the rotating disc 7 can all reach the position aligned with the light source, so that the light generated by the light source can enter the corresponding filter hole 8 and then be filtered by the filter.

[0032] A zero position hole 10 is arranged on the outer ring of the rotating disc 7. The zero position hole 10 is a groove structure arranged on the rotating disc 7. A sensor 9 is arranged on the radial outer portion of the rotating disc 7. Specifically, a fiber mounting seat 20 is arranged on the base 1. A sensor mounting plate 21 is arranged on the fiber mounting seat 20. The fiber mounting seat 20 is arranged on the side of the rotating disc 7 away from the light source assembly. One end of the sensor mounting plate 21 is fixedly connected with the fiber mounting seat 20, and the other end of the sensor mounting plate 21 extends to the side of the rotating disc 7 close to the light source assembly. The sensor 9 is an optical switch and is arranged on the side of the sensor mounting plate 21 close to the light source assembly. The sensor 9 can detect the position of the zero position hole 10 on the rotating disc 7. Based on the zero position hole 10 as a reference, the rotating angle of the rotating disc 7 can be controlled, so that the corresponding filter hole 8 is aligned with the light source.

[0033] Since only four groups of filters are needed in the present scheme, the filters are preferentially arranged in the filter holes 8 close to the zero position hole 10 on the rotating disc 7. In this way, the rotating angle of the rotating disc 7 can be reduced.

[0034] The optical fiber bundle 12 is arranged on the optical fiber mounting seat 20, and the optical fiber bundle 12 is a one-to-eight optical fiber bundle, which can evenly divide the light after the light filtering device into eight different channels, so that multiple samples can be detected at the same time, and the detection efficiency is greatly improved. One end of the optical fiber bundle 12 is connected with the light filtering device, and the other end of the optical fiber bundle 12 is divided into eight parts and connected with the spectral emission tube seat 11. Eight groups of second light transmission holes 13 are arranged in the spectral emission tube seat 11, and eight terminals of the optical fiber bundle 12 are fixed on the eight second light transmission holes 13 of the spectral emission tube seat 11. A plano-convex mirror is arranged in each second light transmission hole 13, which can converge the light emitted by the optical fiber bundle 12, so that more light is gathered on the sample to be detected. A spectral absorption tube seat 15 is arranged on the other side of the spectral emission tube seat 11, and the sample to be detected is arranged between the spectral emission tube seat 11 and the spectral absorption tube seat 15. After passing through the sample to be detected, the light enters the third light transmission hole 14 of the spectral absorption tube seat 15, and the third light transmission hole 14 is also provided with eight groups of third light transmission holes 14 corresponding to the positions of the second light transmission holes 13. Double convex mirrors are arranged in the third light transmission holes 14, the light passes through the double convex mirrors to produce focusing, and finally passes through a silicon photodiode to generate an analog quantity and obtain experimental results.

Claims

1. An enzyme photometer measuring device, characterized by, The application relates to a light source assembly and a filter device, which comprise a base, a light source assembly and a filter device arranged on the base, the light source assembly is slidably arranged on the base, the light source assembly comprises a mounting seat and a light source, the mounting seat is internally provided with a hollow groove, the light source is arranged at the position corresponding to the hollow groove, and a first light transmission hole is arranged in the hollow groove and aligned with the filter device.

2. The device according to claim 1, wherein the device is characterized by, The light source comprises a light source mounting plate, the light source mounting plate is provided with a lamp body and a heat dissipation fin, and the light source mounting plate is arranged between the mounting seat and the heat dissipation fin.

3. The device of claim 2, wherein the device is a microplate reader assay device. The filter device comprises a rotating disc, a plurality of filter holes are uniformly distributed on the rotating disc in a ring shape, and filter pieces are arranged in the filter holes.

4. The device of claim 3, wherein the device is a microplate reader assay device. The filter holes and the lamp body are located on the same axis.

5. The device of claim 3, wherein the device is a microplate reader assay device. The filter device further comprises a sensor located on the ring-shaped outer portion of the rotating disc, and the outer ring of the rotating disc is further provided with a zero hole.

6. The device of claim 5, wherein the device is a microplate reader assay device. The filter pieces are arranged in the filter holes of the rotating disc close to the zero hole.

7. The device according to any one of claims 1 to 6, wherein the device is a microplate reader device. The application further comprises a spectrum emission tube seat, the spectrum emission tube seat is provided with a second light transmission hole, and a plano-convex mirror is arranged in the second light transmission hole.

8. The assay device of claim 7, wherein the assay device is an enzyme-linked immunosorbent assay (ELISA) device. The application further comprises a spectrum absorption tube seat, the spectrum absorption tube seat is provided with a third light transmission hole, and a double-convex mirror is arranged in the third light transmission hole.

9. The device of claim 7, wherein the device is a microplate reader assay device. The optical fiber bundle is a one-to-eight optical fiber bundle.

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