Cell culture instrument for immune cell amplification

Abstract

The utility model relates to the field of cell culture equipment, in particular to a cell culture instrument for amplifying immune cells, which comprises a culture box body, and the heating pipes are mounted on two sides in the incubator body and are used for internal heating. According to the cell culture instrument for immune cell amplification disclosed by the utility model, through the arrangement of the culture box body, the heating pipe, the temperature sensor, the humidity sensor, the control box, the processor, the humidifier and the spraying assembly, the temperature and the humidity in the culture box body are detected through the temperature sensor and the humidity sensor; detected data are transmitted to the processor, then the data are transmitted to the heating pipe through the processor, constant temperature control is conducted on the interior, and when the humidity in the incubator body is higher than the highest threshold value, the processor controls the humidifier to convey moisture to be sprayed out of the spraying assembly, so that the humidity and temperature in the incubator body are controlled; therefore, the internal environment reaches the most suitable culture environment.

Description

Technical Field

[0001] This utility model relates to the field of cell culture equipment, specifically to a cell culture instrument for the amplification of immune cells. Background Technology

[0002] Immune cells are cells that participate in or are related to the immune response. These include lymphocytes, dendritic cells, monocytes / macrophages, granulocytes, and mast cells. Immune cells are cultured using specialized equipment.

[0003] A search revealed a utility model cell culture instrument for immune cell amplification, with publication number CN212404118U. The instrument includes a hollow culture chamber with a carbon dioxide inlet on its side wall. An opening door is provided on the culture chamber, connected to it via a rotating shaft. The upper part of the opening door is a glass window. A partition is positioned horizontally inside the culture chamber corresponding to the glass window. In this cell culture instrument for immune cell amplification, when carbon dioxide precipitates within the culture chamber, a motor drives a connecting rod via a transmission belt. Through the transmission of the connecting rod and a cam, a stirring shaft rotates, causing a stirring blade to rotate. The stirring blade agitates the precipitated carbon dioxide, allowing some of it to flow back to the upper layer through a guide tube. This slows down the sedimentation rate of the carbon dioxide and increases the practicality of the device.

[0004] Existing cell culture processes have high requirements for temperature, humidity, and CO2 concentration. Currently available immune cell culture devices can only perform simple control operations and cannot automatically adjust the internal environment settings. In the comparative case above, the cell culture instrument for immune cell expansion cannot automatically control the internal temperature and humidity during the immune cell culture process, thus affecting the culture of immune cells.

[0005] Therefore, it is necessary to invent a cell culture instrument for the expansion of immune cells to solve the above problems. Utility Model Content

[0006] The purpose of this invention is to provide a cell culture instrument for immune cell amplification. It uses temperature and humidity sensors to detect the temperature and humidity inside the culture chamber. When the temperature is too high or too low, the detected data is transmitted to a processor, which then transmits the data to a heating element for constant temperature control. When the humidity inside the culture chamber exceeds a maximum threshold, the processor controls a humidifier to deliver moisture from a spray assembly, thereby controlling the internal humidity and temperature to achieve the most suitable culture environment, thus solving the problems mentioned in the background art.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a cell culture instrument for immune cell amplification, comprising a culture chamber;

[0008] Heating tubes are installed on both sides inside the incubator for internal heating. An outer door is movably connected to one side of the incubator, and an inner door is provided inside the outer door. Grooves are opened on both sides of the inside of the incubator, and the heating tubes are installed inside the grooves. A partition is fixedly installed on the outside of the grooves. Exhaust fans are installed on both sides of the inside of the incubator, and a frame is fixedly installed at the bottom inside the incubator.

[0009] Temperature and humidity sensors are installed inside the upper part of the incubator to detect the internal temperature and humidity. A control box is installed above the incubator, and a processor is installed inside the control box. A humidifier is installed on one side of the processor, and a spray assembly is fixedly connected to one side of the humidifier.

[0010] A placement rack, installed inside the incubator, is used to place cell culture flasks. The placement rack is equipped with a limiting component inside to hold the cell culture flasks.

[0011] Preferably, two sets of ultraviolet germicidal lamps are fixedly installed on the upper sides of the interior of the incubator.

[0012] Preferably, the input terminals of the temperature sensor and humidity sensor are electrically connected to the output terminal of the processor, and the input terminal of the processor is electrically connected to the output terminal of the humidifier and heating tube.

[0013] Preferably, a servo motor is installed inside the frame, a first gear is fixedly installed on one side of the servo motor, a second gear is movably connected to one side of the first gear, a transmission rod is fixedly installed above the second gear, and the placement frame is installed outside the transmission rod.

[0014] Preferably, a baffle is fixedly installed on the outside of the placement rack, and placement grooves are formed on the inner surface of the placement rack.

[0015] Preferably, the limiting component includes a spring, a clamping plate, and a sponge pad. The spring is installed on both sides inside the placement groove, a clamping plate is fixedly installed on one side of the spring, and a sponge pad is pasted on the outside of the clamping plate.

[0016] The technical effects and advantages provided by this utility model in the above technical solution are as follows:

[0017] 1. By setting up an incubator, heating tube, temperature sensor, humidity sensor, control box, processor, humidifier, and spray assembly, the temperature and humidity inside the incubator can be controlled, improving the cell culture effect. The temperature and humidity sensors detect the temperature and humidity inside the incubator. When the temperature is too high or too low, the detected data is transmitted to the processor, which then transmits the data to the heating tube for constant temperature control. When the humidity inside the incubator exceeds the maximum threshold, the processor controls the humidifier to deliver moisture from the spray assembly, thereby controlling the internal humidity and temperature to achieve the most suitable culture environment.

[0018] 2. The servo motor, first gear, second gear, transmission rod, placement rack, baffle, placement slot, and limiting components facilitate the placement and retrieval of cell culture flasks by medical staff. They also provide limiting clamping for the cell culture flasks, improving placement stability. Turning on the servo motor activates the first and second gears, causing the transmission rod and placement rack to rotate. This allows medical staff to place the cell culture flasks in batches into the placement slots within the rack. The springs and clamps within the placement slots provide limiting clamping for the cell culture flasks, further enhancing stability. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0020] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0021] Figure 2 This is a schematic diagram of the internal structure of the incubator of this utility model;

[0022] Figure 3 This is a schematic diagram of the placement rack structure of this utility model;

[0023] Figure 4 This is a schematic diagram of the internal structure of the placement rack of this utility model;

[0024] Figure 5 This is a schematic diagram of the limiting component structure of this utility model.

[0025] Explanation of reference numerals in the attached figures:

[0026] 1. Incubator body; 2. Outer door; 3. Inner door; 4. Groove; 5. Heating element; 6. Partition; 7. Exhaust fan; 8. Temperature sensor; 9. Humidity sensor; 10. Ultraviolet germicidal lamp; 11. Control box; 12. Processor; 13. Humidifier; 14. Spray assembly; 15. Frame; 16. Servo motor; 17. First gear; 18. Second gear; 19. Transmission rod; 20. Placement rack; 21. Baffle; 22. Placement slot; 23. Limiting assembly; 2301. Spring; 2302. Clamping plate; 2303. Sponge pad. Detailed Implementation

[0027] To enable those skilled in the art to better understand the technical solution of this utility model, the present utility model will be further described in detail below with reference to the accompanying drawings.

[0028] This utility model provides, for example Figure 1-5 A cell culture instrument for the expansion of immune cells is shown, including a culture chamber 1;

[0029] Heating tubes 5 are installed on both sides inside the culture chamber 1 for internal heating. An outer door 2 is movably connected to one side of the culture chamber 1. An inner door 3 is provided inside the outer door 2. Grooves 4 are opened on both sides of the inside of the culture chamber 1. Heating tubes 5 are installed inside the grooves 4. A partition 6 is fixedly installed on the outside of the grooves 4. Exhaust fans 7 are installed on both sides of the inside of the culture chamber 1. A frame 15 is fixedly installed at the bottom inside the culture chamber 1.

[0030] Temperature sensor 8 and humidity sensor 9 are installed inside the upper part of the incubator 1 to detect the internal temperature and humidity. A control box 11 is installed on the upper part of the incubator 1. A processor 12 is installed inside the control box 11. A humidifier 13 is installed on one side of the processor 12. A spray assembly 14 is fixedly connected to one side of the humidifier 13.

[0031] A placement rack 20 is installed inside the culture chamber 1 to hold cell culture flasks. The placement rack 20 has a limiting component 23 inside to hold the cell culture flasks. Temperature and humidity inside the culture chamber 1 are detected by temperature sensor 8 and humidity sensor 9. When the temperature is too high or too low, the detected data is transmitted to processor 12, which then transmits the data to heating tube 5 for constant temperature control. When the humidity inside the culture chamber 1 is higher than the maximum threshold, processor 12 controls humidifier 13 to deliver moisture from spray component 14, thereby controlling the internal humidity and temperature to achieve the most suitable culture environment.

[0032] like Figure 1 and Figure 2As shown, ultraviolet germicidal lamps 10 are fixedly installed on both sides of the upper part of the culture chamber 1. There are two sets of ultraviolet germicidal lamps 10. When cell culture bottles need to be placed, the ultraviolet germicidal lamps 10 first sterilize the inside of the culture chamber 1, thereby maintaining the sterile state inside the culture chamber 1.

[0033] like Figure 2 As shown, the input terminals of temperature sensor 8 and humidity sensor 9 are electrically connected to the output terminal of processor 12. The input terminal of processor 12 is electrically connected to the output terminals of humidifier 13 and heating tube 5. Through temperature sensor 8 and humidity sensor 9, the temperature and humidity inside the incubator 1 can be detected in real time, and the data is transmitted to heating tube 5 and humidifier 13 to make the internal environment reach the most suitable incubation environment.

[0034] like Figure 1 , Figure 3 and Figure 4 As shown, a servo motor 16 is installed inside the frame 15. A first gear 17 is fixedly installed on one side of the servo motor 16. A second gear 18 is movably connected to one side of the first gear 17. A transmission rod 19 is fixedly installed above the second gear 18. The placement rack 20 is installed outside the transmission rod 19. By turning on the servo motor 16, the first gear 17 and the second gear 18 are driven to mesh and drive the transmission rod 19 and the placement rack 20 to rotate, so that medical staff can place cell culture flasks in batches in the placement rack 20.

[0035] like Figure 1 , Figure 3 and Figure 4 As shown, a baffle 21 is fixedly installed on the outside of the placement rack 20, and placement slots 22 are opened on the inner surface of the placement rack 20. The placement slots 22 facilitate the placement of cell culture flasks.

[0036] like Figure 4 and Figure 5 As shown, the limiting component 23 includes a spring 2301, a clamping plate 2302, and a sponge pad 2303. The spring 2301 is installed on both sides inside the placement groove 22. The clamping plate 2302 is fixedly installed on one side of the spring 2301. The sponge pad 2303 is pasted on the outside of the clamping plate 2302. By using the spring 2301 and the clamping plate 2302 in the placement groove 22, the cell culture flask can be limited and clamped, which improves the stability.

[0037] The working principle of this utility model is as follows: First, connect the external power supply. Then, turn on the switch of the ultraviolet germicidal lamp 10 inside the incubator 1. The ultraviolet germicidal lamp 10 sterilizes the inside of the incubator 1. After sterilization, turn off the switch of the ultraviolet germicidal lamp 10. Then, open the outer door 2 and inner door 3 of the incubator 1. Place the prepared cell culture flasks into the placement slots 22 inside the placement rack 20 as needed. The springs 2301 and clamps 2302 in the placement slots 22 can limit and hold the cell culture flasks, improving stability. Then, turn on the servo motor 16, which drives the first gear 17 and the second gear 18 to mesh and drive the transmission rod 19 and the placement rack 20 to rotate, making it easier for medical staff to place the cell culture flasks in sequence. Place the flasks in the placement slots 22 of the other placement racks 20. After placing the cell culture flasks, turn off the servo motor 16 switch, and then close the inner door 3 and the outer door 2. When the temperature sensor 8 detects that the internal temperature of the culture chamber 1 is too high or too low, the detected data is transmitted to the processor 12. The processor 12 then transmits the data to the heating tube 5, thereby turning on the heating tube 5 switch to maintain a constant temperature inside. When the humidity sensor 9 inside the culture chamber 1 detects that the humidity is higher than the maximum threshold, the processor 12 controls the humidifier 13 to deliver moisture from the spray assembly 14, thereby controlling the internal humidity and making the internal environment reach the most suitable culture environment. In this way, the use of the cell culture instrument for immune cell amplification is completed.

[0038] The foregoing description only illustrates certain exemplary embodiments of the present invention. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A cell culture instrument for expanding immune cells, characterized in that: Including the incubator (1); Heating tubes (5) are installed on both sides inside the culture chamber (1) for internal heating. An outer door (2) is movably connected to one side of the culture chamber (1). An inner door (3) is provided inside the outer door (2). Grooves (4) are opened on both sides of the inside of the culture chamber (1). The heating tubes (5) are installed inside the grooves (4). A partition (6) is fixedly installed on the outside of the grooves (4). Exhaust fans (7) are installed on both sides inside the culture chamber (1). A frame (15) is fixedly installed at the bottom inside the culture chamber (1). Temperature sensor (8) and humidity sensor (9) are installed inside the upper part of the incubator (1) to detect the internal temperature and humidity. A control box (11) is installed above the incubator (1). A processor (12) is installed inside the control box (11). A humidifier (13) is installed on one side of the processor (12). A spray assembly (14) is fixedly connected to one side of the humidifier (13). A placement rack (20) is installed inside the culture chamber (1) for placing cell culture flasks. The placement rack (20) is provided with a limiting component (23) for holding the cell culture flasks.

2. The cell culture instrument for immune cell expansion according to claim 1, characterized in that: Ultraviolet germicidal lamps (10) are fixedly installed on both sides of the upper part of the incubator (1), and two sets of ultraviolet germicidal lamps (10) are provided.

3. The cell culture instrument for immune cell amplification according to claim 1, characterized in that: The input terminals of the temperature sensor (8) and humidity sensor (9) are electrically connected to the output terminal of the processor (12), and the input terminal of the processor (12) is electrically connected to the output terminals of the humidifier (13) and heating tube (5).

4. The cell culture instrument for immune cell expansion according to claim 1, characterized in that: The frame (15) is equipped with a servo motor (16) inside. A first gear (17) is fixedly installed on one side of the servo motor (16). A second gear (18) is movably connected to one side of the first gear (17). A transmission rod (19) is fixedly installed above the second gear (18). The placement rack (20) is installed outside the transmission rod (19).

5. A cell culture instrument for immune cell amplification according to claim 1, characterized in that: The placement rack (20) is fixedly installed with a baffle (21) on the outside, and the internal surface of the placement rack (20) is provided with a placement groove (22).

6. A cell culture instrument for immune cell amplification according to claim 1, characterized in that: The limiting component (23) includes a spring (2301), a clamping plate (2302), and a sponge pad (2303). The spring (2301) is installed on both sides inside the placement groove (22). The clamping plate (2302) is fixedly installed on one side of the spring (2301), and the sponge pad (2303) is pasted on the outside of the clamping plate (2302).

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