Cell culture bottle convenient for changing liquid

Abstract

The utility model relates to the technical field of cell culture, in particular to a cell culture bottle convenient for changing liquid, which comprises a bottle body, a bottle opening is fixedly mounted at the upper end of the bottle body, a first bottle cap is sleeved at the upper end of the bottle opening through threads, the bottle opening is inclined forwards, a liquid changing tube is fixedly mounted in the right end of the bottle body, and a second bottle cap is sleeved at the right end of the bottle body. A second bottle cap is arranged at the upper end of the liquid changing pipe, a one-way assembly is arranged at the lower end of the liquid changing pipe, and a partition assembly is arranged at the upper end of the liquid changing pipe. Through the design of the one-way assembly, when a culture solution is added or replaced, pollutants in external air can be effectively prevented from entering the culture bottle, so that the sterile state of a culture environment is maintained, and the situation that the cell culture bottle topples and leaks when the culture solution is injected into the cell culture bottle is avoided through the arrangement of the separation assembly; on the whole, the cell culture bottle disclosed by the utility model is simple in structure and convenient to operate.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, specifically to a cell culture flask that facilitates medium replacement. Background Technology

[0002] In vitro culture of cells and tissues has become an indispensable part of life research and practice. There are many types of cells to be cultured, from viruses to bacteria and fungi, from human cells to animal cells and plant cells. Therefore, cell culture flasks are needed to culture cells. Some mammalian cells require surface adhesion. Therefore, in addition to having good transparency and being non-toxic and sterile, culture flasks, culture plates and culture dishes used to provide the in vitro culture environment for cells also need to undergo surface modification treatment to enable them to adhere, divide and grow.

[0003] During cell culture, adherent cells typically grow in culture flasks. When changing the culture medium, the conventional practice is to pour out the old medium and then add new medium. However, if the pouring angle is too large, a large number of cells may be lost with the culture medium, thus affecting the accuracy of experimental data. Another method is to use a dropper to remove the old culture medium, but this method carries the risk of cell rupture due to the dropper touching the flask wall, and it is also not very convenient to operate. Therefore, we propose a cell culture flask that is easy to change medium to solve the above problems. Utility Model Content

[0004] The purpose of this invention is to provide a cell culture flask that facilitates medium replacement, in order to solve the problems mentioned in the background art, such as the excessive tilting angle which may cause a large number of cells to be lost with the culture medium, thus affecting the accuracy of experimental data, and the use of a dropper to aspirate the old culture medium, but this method has the risk of cell rupture caused by the dropper touching the flask wall, and is not convenient to operate.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a cell culture flask for easy medium replacement, comprising a flask body, a flask mouth fixedly installed at the upper end of the flask body, a first cap threadedly connected to the upper end of the flask mouth, the flask mouth being inclined forward, a medium replacement tube fixedly installed inside the right end of the flask body, a second cap provided at the upper end of the medium replacement tube, a one-way component provided at the lower end of the medium replacement tube, and a separator component provided at the upper end of the medium replacement tube.

[0006] Preferably, the unidirectional component includes a connecting tube, which is located in the middle of the liquid exchange tube inside the bottle. A sleeve is fixedly installed inside the connecting tube, and a movable groove is opened inside the sleeve. A spring is installed in the movable groove, and a connecting rod is movably installed at the upper opening of the movable groove. A plug is fixedly installed at the upper end of the connecting rod, and the plug is movably inserted into the liquid exchange tube.

[0007] Preferably, the inner diameter of the connecting pipe is larger than the inner diameter of the fluid exchange pipe, and the outer diameter of the plug is the same as the inner diameter of the fluid exchange pipe.

[0008] Preferably, the lower end of the liquid exchange tube has a notch, and the lower end of the liquid exchange tube is in contact with the inner front wall of the bottle.

[0009] Preferably, a writing pad is provided on the front side of the bottle, and the surface of the writing pad is provided with a waterproof layer.

[0010] Preferably, a filter plate is provided inside the upper end of the liquid exchange tube, and the filter plate is located below the separator component at the upper end of the liquid exchange tube.

[0011] Preferably, the separating component includes a partition plate, which is fixed inside the liquid exchange tube. A baffle plate is rotatably installed at the upper end of the liquid exchange tube. Fixing plates are symmetrically installed on the left and right sides of the upper opening of the liquid exchange tube. Bolts are threaded into both sets of fixing plates. A drain port is opened on the left side of the upper end of the liquid exchange tube, and the drain port is in contact with the upper inner wall of the bottle.

[0012] Preferably, the lower end of the partition is located inside the liquid exchange pipe on the upper side of the connecting pipe, and the drain outlet is located on the left side of the middle part of the partition.

[0013] Compared with the prior art, the beneficial effects of this utility model are: the design of the unidirectional component of this utility model can effectively prevent pollutants in the outside air from entering the culture bottle when adding or replacing culture medium, thereby maintaining the sterile state of the culture environment. The setting of the separator component avoids the cell culture bottle from tipping over and causing leakage when injecting culture medium into the cell culture bottle. Overall, the cell culture bottle of this utility model has a simple structure and is easy to operate. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a front view schematic diagram of the structure of this utility model;

[0016] Figure 2 This is a front sectional view of the structure of this utility model;

[0017] Figure 3 This is a schematic diagram of the structure of this utility model from the right side view.

[0018] Figure 4This utility model Figure 2 A magnified view of part A in the diagram;

[0019] Figure 5 This utility model Figure 2 A magnified view of part B in the diagram.

[0020] In the diagram: 1. Bottle body; 2. Bottle mouth; 3. First cap; 4. Liquid exchange tube; 5. Second cap; 6. Connecting tube; 7. Sleeve; 8. Movable groove; 9. Spring; 10. Connecting rod; 11. Block; 12. Filter plate; 13. Partition; 14. Baffle; 15. Fixing plate; 16. Bolt; 17. Notch; 18. Drain; 19. Writing plate. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Please see Figure 1-5 This utility model provides an embodiment of a cell culture flask that facilitates medium replacement, comprising a flask body 1, a flask mouth 2 fixedly installed at the upper end of the flask body 1, a first flask cap 3 threadedly connected to the upper end of the flask mouth 2, the flask mouth 2 being tilted forward, a medium replacement tube 4 fixedly installed inside the right end of the flask body 1, a second flask cap 5 provided at the upper end of the medium replacement tube 4, a one-way component provided at the lower end of the medium replacement tube 4, and a separator component provided at the upper end of the medium replacement tube 4. This device, through the provided one-way component and separator component, prevents leakage when the flask body 1 is tilted when injecting culture medium, and can effectively prevent pollutants in the outside air from entering the culture flask.

[0023] Furthermore, the one-way component includes a connecting pipe 6, which is located in the middle of the fluid exchange tube 4 inside the bottle body 1. A sleeve 7 is fixedly installed inside the connecting pipe 6, and a movable groove 8 is opened inside the sleeve 7. A spring 9 is installed in the movable groove 8, and a connecting rod 10 is movably installed at the upper opening of the movable groove 8. A plug 11 is fixedly installed at the upper end of the connecting rod 10, and the plug 11 is movably inserted into the fluid exchange tube 4. When the culture medium is injected into the fluid exchange tube 4, the culture medium exerts pressure on the connecting rod 10 and the plug 11, causing the connecting rod 10 and the plug 11 to slide into the sleeve 7. The culture medium in the upper end of the fixed fluid exchange tube 4 is injected into the bottle body 1 from the connecting pipe 6 and the lower end of the fluid exchange tube 4, thus preventing backflow when injecting the culture medium.

[0024] Furthermore, the inner diameter of the connecting tube 6 is larger than that of the inner diameter of the liquid exchange tube 4, and the outer diameter of the plug 11 is the same as that of the inner diameter of the liquid exchange tube 4. This structure, based on the inner wall sizes of the connecting tube 6 and the liquid exchange tube 4, allows the culture medium in the liquid exchange tube 4 to flow downward within the connecting tube 6 when the connecting rod 10 and the filter plate 12 slide into the connecting tube 6.

[0025] Furthermore, a notch 17 is provided at the lower end of the fluid exchange tube 4, and the lower end of the fluid exchange tube 4 is attached to the inner front wall of the bottle body 1. This structure, based on the notch 17 provided at the lower end of the fluid exchange tube 4 and the attachment of the lower end of the fluid exchange tube 4 to the inner front wall of the bottle body 1, ensures that when the culture medium is injected into the bottle body 1, the culture medium flowing in the fluid exchange tube 4 flows downward on the inner front wall of the bottle body 1, avoiding impact on the adherent cells on the inner rear wall of the bottle body 1.

[0026] Furthermore, a writing pad 19 is provided on the front side of the bottle body 1, and a waterproof layer is provided on the surface of the writing pad 19. This structure, through the writing pad 19 provided on the front side of the bottle body 1, facilitates researchers to record experimental information, such as experimental date, cell type, etc., to ensure the accuracy and traceability of experimental data.

[0027] Furthermore, a filter plate 12 is provided at the upper end of the liquid exchange tube 4. The filter plate 12 is located below the separator component at the upper end of the liquid exchange tube 4. When the culture medium is injected into the bottle 1, the culture medium injected into the liquid exchange tube 4 is filtered by the filter plate 12, which can effectively block the invasion of external microorganisms and particulate matter, while allowing the culture medium to pass through smoothly.

[0028] Furthermore, the separation assembly includes a partition 13, which is fixed inside the fluid exchange tube 4. A baffle 14 is rotatably mounted on the upper end of the fluid exchange tube 4. Fixing plates 15 are symmetrically installed on both sides of the upper opening of the fluid exchange tube 4. Bolts 16 are threaded into both sets of fixing plates 15. A drain port 18 is opened on the upper left side of the fluid exchange tube 4, and the drain port 18 is in contact with the upper inner wall of the bottle body 1. This structure, by tilting the baffle 14 left and right at the upper end of the fluid exchange tube 4, separates the inner walls of the fluid exchange tube 4 on both sides of the partition 13, forming independent liquid flow spaces on both sides of the partition 13. This design reduces the risk of cross-contamination during cell culture.

[0029] Furthermore, the lower end of the partition 13 is located inside the liquid exchange tube 4 on the upper side of the connecting tube 6, and the drain port 18 is located on the left side of the middle part of the partition 13. This structure allows the culture medium inside the bottle 1 to flow out from the drain port 18 when the bottle 1 is inverted. And by separating the inner wall of the right side of the liquid exchange tube 4 through the partition 13, it can be ensured that the culture medium flowing into the liquid exchange tube 4 from the drain port 18 enters the space on the right side of the liquid exchange tube 4 separated by the partition 13.

[0030] Working principle: When adding or changing the culture medium, such as Figure 2 , Figure 3 , Figure 4 and Figure 5 As shown, remove the second cap 5 from the fluid exchange tube 4, attach the baffle 14 to the inner wall of the upper left side of the fluid exchange tube 4, and then tighten the bolt 16 to fix the baffle 14 in place. Use a pipette to inject the culture medium into the fluid exchange tube 4 from the right side of the baffle 14. After entering the fluid exchange tube 4, the culture medium flows downward and exerts pressure on the plug 11. The connecting rod 10 and the plug 11 slide into the movable groove 8 on the lower sleeve 7. The culture medium in the fluid exchange tube 4 continues to flow downward through the connecting tube 6. The culture medium flows from the lower end of the fluid exchange tube 4. The culture medium flows out of the notch 17 and flows downward on the inner wall of the front side of the bottle body 1. When the block 11 no longer feels pressure, the connecting rod 10 and the block 11 slide and reset under the action of the spring 9, closing the inner wall of the liquid exchange tube 4. The baffle 14 is moved to the right, and then the bolt 16 is turned to limit and fix the baffle 14. The bottle body 1 is turned upside down, and the culture medium in the bottle body 1 enters the liquid exchange tube 4 from the drain port 18. Then the culture medium flows out from the space on the left side of the liquid exchange tube 4 separated by the partition 13 and the baffle 14. The above is the entire working principle of this utility model.

[0031] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A cell culture flask that facilitates medium changes, comprising a flask body (1), characterized in that: The bottle body (1) is fixedly installed with a bottle mouth (2), and the upper end of the bottle mouth (2) is threaded with a first bottle cap (3). The bottle mouth (2) is tilted forward. The right end of the bottle body (1) is fixedly installed with a liquid replacement tube (4). The upper end of the liquid replacement tube (4) is provided with a second bottle cap (5). The lower end of the liquid replacement tube (4) is provided with a one-way component. The upper end of the liquid replacement tube (4) is provided with a separator component.

2. The cell culture flask for easy medium change according to claim 1, characterized in that: The one-way component includes a connecting pipe (6), which is located in the middle of the liquid exchange pipe (4) inside the bottle (1). A sleeve (7) is fixedly installed inside the connecting pipe (6). A movable groove (8) is opened inside the sleeve (7). A spring (9) is installed inside the movable groove (8). A connecting rod (10) is movably installed at the upper opening of the movable groove (8). A block (11) is fixedly installed at the upper end of the connecting rod (10). The block (11) is movably inserted into the liquid exchange pipe (4).

3. A cell culture flask for easy medium change according to claim 2, characterized in that: The inner diameter of the connecting pipe (6) is larger than the inner diameter of the liquid exchange pipe (4), and the outer diameter of the plug (11) is the same as the inner diameter of the liquid exchange pipe (4).

4. A cell culture flask for easy medium change according to claim 1, characterized in that: The lower end of the liquid exchange tube (4) is provided with a notch (17), and the lower end of the liquid exchange tube (4) is attached to the inner front wall of the bottle body (1).

5. A cell culture flask for easy medium change according to claim 1, characterized in that: A writing pad (19) is provided on the front side of the bottle body (1), and a waterproof layer is provided on the surface of the writing pad (19).

6. A cell culture flask for easy medium change according to claim 1, characterized in that: A filter plate (12) is provided inside the upper end of the liquid exchange pipe (4), and the filter plate (12) is located below the separator component at the upper end of the liquid exchange pipe (4).

7. A cell culture flask for easy medium change according to claim 1, characterized in that: The separation assembly includes a partition (13), which is fixed inside the liquid exchange tube (4). A baffle (14) is rotatably installed on the upper end of the liquid exchange tube (4). Fixing plates (15) are symmetrically installed on the left and right sides of the upper opening of the liquid exchange tube (4). Bolts (16) are threadedly inserted into both sets of fixing plates (15). A drain port (18) is opened on the upper left side of the liquid exchange tube (4). The drain port (18) is attached to the upper inner wall of the bottle body (1).

8. A cell culture flask for easy medium change according to claim 7, characterized in that: The lower end of the partition (13) is located inside the liquid exchange pipe (4) on the upper side of the connecting pipe (6), and the drain port (18) is located on the left side of the middle part of the partition (13).

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