Underwater multi-wavelength excitation luminoscope

Abstract

The utility model relates to the technical field of fluorescence detection equipment, and provides an underwater multi-wavelength excitation luminoscope which comprises a shell, an annular multi-wavelength collimation light source, a condensing lens, an optical filter, a concave grating and a photoelectric detector are sequentially arranged in the shell, and a processing system is further arranged in the shell. The processing system comprises a DSP digital signal processor, a master control module, a communication module and an upper computer which are electrically connected in sequence. The photoelectric detector is electrically connected with the DSP digital signal processor. On the basis, detection of fluorescence with different wavelengths can be realized.

Description

Technical Field

[0001] This application relates to the field of fluorescence detection equipment, and in particular to an underwater multi-wavelength excitation fluorescence instrument. Background Technology

[0002] Traditional underwater fluorescence detection instruments typically can only detect a single range of fluorescence wavelengths, or a very limited range, which restricts their ability to identify and detect different fluorescent markers. In complex underwater environments, multiple fluorescent substances may exist, and a single detection wavelength cannot meet the needs of simultaneous analysis of multiple components.

[0003] Therefore, this application studies an underwater multi-wavelength excitation fluorescence instrument, which enables the detection of fluorescence at different wavelengths. Utility Model Content

[0004] In order to enable the detection of fluorescence at different wavelengths, this application provides an underwater multi-wavelength excitation fluorescence instrument.

[0005] This application provides an underwater multi-wavelength excitation fluorescence instrument, which adopts the following technical solution:

[0006] An underwater multi-wavelength excitation fluorescence instrument includes a housing. Inside the housing, a ring-shaped multi-wavelength collimating light source, a focusing lens, a filter, a concave grating, and a photodetector are arranged sequentially. The housing also includes a processing system, which comprises a DSP digital signal processor, a main control module, a communication module, and a host computer that are electrically connected in sequence. The photodetector is electrically connected to the DSP digital signal processor.

[0007] By adopting the above technical solution, a ring-shaped multi-wavelength collimated light source emits light of multiple wavelengths. This light is used to excite fluorescent substances in the water. A specific wavelength of excitation light is selected by a filter, and the excitation light is focused by a condenser lens and irradiates the water sample, exciting the fluorescent substances in the water sample to emit fluorescence. The fluorescence is dispersed into light of different wavelengths by a concave grating and focused onto a photodetector. The photodetector converts the fluorescence signal into an electrical signal and transmits it to a DSP digital signal processor for processing. The DSP digital signal processor filters, amplifies, and digitizes the signal, and then transmits it to the main control module. The main control module transmits the processed data to the communication module, and the communication module then transmits it to the host computer. The host computer processes, analyzes, and visualizes the received data to obtain information about the content and characteristics of fluorescent substances in the water sample. Therefore, it is possible to detect fluorescence of different wavelengths.

[0008] Optionally, the annular multi-wavelength collimated light source includes a ring of uniformly distributed collimated LED beads of various wavelengths.

[0009] By adopting the above technical solution, the light source adopts a ring design, which allows the LED beads to be evenly distributed on the ring structure, ensuring uniform light distribution. Since the ring light source has a variety of LED beads of different wavelengths, the light emitted by them will be superimposed on the target area to form mixed light of multiple wavelengths, which can excite a variety of fluorescent substances in the water sample, thereby improving the accuracy of measurement.

[0010] Optionally, the centerline of the focusing lens is on the same straight line as the centerline of the annular multi-wavelength collimating light source.

[0011] By adopting the above technical solution, it is possible to better focus the fluorescence and transfer it to the spherical cylindrical grating.

[0012] Optionally, a narrow-band filter is provided in front of the photodetector.

[0013] Optionally, the upper surface of the concave grating is inclined and faces the filter side.

[0014] Optionally, the housing is also provided with a waterproof charging interface.

[0015] Optionally, the processing system may further include a light source control module for controlling the LED beads to emit light.

[0016] Optionally, the processing system may further include a battery control module for controlling charging or discharging.

[0017] In summary, this application includes at least one of the following beneficial technical effects:

[0018] 1. A ring-shaped multi-wavelength collimated light source emits light of various wavelengths, which are used to excite fluorescent substances in the water. A specific wavelength of excitation light is selected by a filter, and the excitation light is focused by a condenser lens and irradiates the water sample, exciting the fluorescent substances in the water sample to emit fluorescence. The fluorescence is dispersed into light of different wavelengths by a concave grating and focused onto a photodetector. The photodetector converts the fluorescence signal into an electrical signal and transmits it to a DSP digital signal processor for processing. The DSP digital signal processor filters, amplifies, and digitizes the signal, and then transmits it to the main control module. The main control module transmits the processed data to the communication module, and the communication module then transmits it to the host computer. The host computer processes, analyzes, and visualizes the received data to obtain information about the content and characteristics of fluorescent substances in the water sample, thus enabling the detection of fluorescence at different wavelengths.

[0019] 2. The light source adopts a ring design, which allows the LED beads to be evenly distributed on the ring structure, ensuring uniform light distribution. Since the ring light source has multiple LED beads of different wavelengths, the light emitted by them will be superimposed on the target area to form mixed light of multiple wavelengths, which can excite multiple fluorescent substances in the water sample, thereby improving the accuracy of measurement. Attached Figure Description

[0020] Figure 1 This is a system diagram of the underwater multi-wavelength excitation fluorescence instrument according to an embodiment of this application;

[0021] Figure 2 This is a plan view of the annular multi-wavelength collimated light source in the underwater multi-wavelength excitation fluorescence instrument of this application embodiment;

[0022] Figure 3 This is a schematic diagram of the structure of the underwater multi-wavelength excitation fluorescence instrument in this application, showing the concave grating. Detailed Implementation

[0023] The following is in conjunction with the appendix Figure 1-3 This application will be described in further detail.

[0024] This application discloses an underwater multi-wavelength excitation fluorescence instrument. (Refer to...) Figure 1 The underwater multi-wavelength excitation fluorescence instrument includes a housing, inside which are arranged sequentially an annular multi-wavelength collimating light source, a condenser lens, a filter, a concave grating, and a photodetector. The annular multi-wavelength collimating light source emits light of multiple wavelengths, which are used to excite fluorescent substances in the water. The filter selects a specific wavelength of excitation light, which is then focused by the condenser lens and irradiates the water sample, exciting the fluorescent substances in the water sample to emit fluorescence. The fluorescence is dispersed into light of different wavelengths by the concave grating and focused onto the photodetector.

[0025] The housing also houses a processing system, which includes a DSP digital signal processor, a main control module, a communication module, and a host computer, all electrically connected in sequence. The photodetector is electrically connected to the DSP. The photodetector converts the fluorescence signal into an electrical signal and transmits it to the DSP for processing. The DSP filters, amplifies, and digitizes the signal before transmitting it to the main control module. The main control module then transmits the processed data to the communication module, which in turn uploads it to the host computer. The host computer processes, analyzes, and visualizes the received data to obtain information about the content and characteristics of fluorescent substances in the water sample, thus enabling the detection of fluorescence at different wavelengths.

[0026] In some embodiments, the annular multi-wavelength collimated light source includes a ring of uniformly distributed collimated LED beads of various wavelengths. In this embodiment, 15 LED beads are used, and each bead has a different wavelength. Therefore, the light emitted by these beads superimposed on the target area, forming a mixed light of multiple wavelengths. This mixed light can excite various fluorescent substances in the water sample, thereby improving the accuracy of the measurement.

[0027] In some embodiments, the centerline of the focusing lens is aligned with the centerline of the annular multi-wavelength collimating light source. This allows for better focusing of the fluorescence and its transmission to the spherical cylindrical grating.

[0028] In some embodiments, a narrow-band filter is provided in front of the photodetector to further exclude fluorescence signals in non-target wavelength bands, making the test more accurate.

[0029] In some embodiments, the upper surface of the concave grating is inclined and faces the filter side. This allows for better dispersion of light into different wavelengths, which are then focused onto the photodetector.

[0030] In some embodiments, the housing is also provided with a waterproof charging port.

[0031] In some embodiments, the processing system further includes a light source control module for controlling the light emission of LED beads. The light source control module sequentially controls LED beads of different wavelengths to emit light, forming a modulated excitation light signal. The modulated excitation light is absorbed by organisms, particulate matter, or dissolved chemicals in the water body detected at the center of the light source, generating a fluorescence signal.

[0032] In some embodiments, the processing system further includes a battery control module for controlling charging or discharging. The battery control module is communicatively connected to the communication module and initiates charging or discharging modes by acquiring the system's power level. The battery control module provides a stable and reliable power supply to the entire instrument. It is also equipped with a power indicator and overcharge / over-discharge protection functions to ensure safe operation of the instrument. In this embodiment, a built-in battery is also provided within the housing, and the built-in battery is controlled by the battery control module.

[0033] The implementation principle of an underwater multi-wavelength excitation fluorescence instrument according to an embodiment of this application is as follows: A ring-shaped multi-wavelength collimated light source emits light of various wavelengths, which are used to excite fluorescent substances in the water. A specific wavelength of excitation light is selected by a filter, and the excitation light is focused by a condenser lens and irradiates the water sample, exciting the fluorescent substances in the water sample to emit fluorescence. The fluorescence is dispersed into light of different wavelengths by a concave grating and focused onto a photodetector. The photodetector converts the fluorescence signal into an electrical signal and transmits it to a DSP digital signal processor for processing. The DSP digital signal processor filters, amplifies, and digitizes the signal, and then transmits it to the main control module. The main control module transmits the processed data to the communication module, and the communication module then transmits it to the host computer. The host computer processes, analyzes, and visualizes the received data to obtain information about the content and characteristics of fluorescent substances in the water sample, thus enabling the detection of fluorescence at different wavelengths.

[0034] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. An underwater multi-wavelength excitation fluorescence instrument, characterized in that: The device includes a housing, within which are sequentially arranged an annular multi-wavelength collimating light source, a focusing lens, a filter, a concave grating, and a photodetector. The housing also includes a processing system comprising a DSP digital signal processor, a main control module, a communication module, and a host computer, all electrically connected in sequence. The photodetector is electrically connected to the DSP digital signal processor.

2. The underwater multi-wavelength excitation fluorescence instrument according to claim 1, characterized in that: The annular multi-wavelength collimated light source includes a ring of uniformly distributed collimated LED beads of various wavelengths.

3. The underwater multi-wavelength excitation fluorescence instrument according to claim 2, characterized in that: The centerline of the focusing lens is on the same straight line as the centerline of the annular multi-wavelength collimating light source.

4. The underwater multi-wavelength excitation fluorescence instrument according to claim 1, characterized in that: A narrow-band filter is placed in front of the photodetector.

5. An underwater multi-wavelength excitation fluorescence instrument according to claim 1, characterized in that: The upper surface of the concave grating is inclined and faces the filter.

6. The underwater multi-wavelength excitation fluorescence instrument according to claim 1, characterized in that: The housing is also equipped with a waterproof charging interface.

7. An underwater multi-wavelength excitation fluorescence instrument according to claim 2, characterized in that: The processing system also includes a light source control module for controlling the light emission of the LED beads.

8. An underwater multi-wavelength excitation fluorescence instrument according to claim 1, characterized in that: The processing system also includes a battery control module for controlling charging or discharging.

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