Airlift bioreactor for culturing adventitious roots of atractylodes macrocephala koidz

Abstract

The utility model discloses an airlift bioreactor for culturing adventitious roots of bighead atractylodes rhizome, and relates to the technical field of adventitious root culture. Comprising a supporting structure and a reactor body, the reactor body is pre-arranged on the supporting structure, a culture solution inlet is formed in the upper end of the reactor body, a sealing piece used for sealing the reactor body is connected to the culture solution inlet, an air inlet is formed in the lower end of the reactor body, an air inlet pipe and a guide pipe are connected to the air inlet, and one end of the guide pipe is connected with the air inlet pipe; the other end of the guide pipe is connected with an oxygen pump, and oxygen is conveyed into the reactor body through the oxygen pump. According to the airlift bioreactor for culturing the adventitious roots of the atractylodes macrocephala, the reactor body is supported through the supporting structure, oxygen is introduced into the reactor body through the oxygen pump, rapid growth of the adventitious roots of the atractylodes macrocephala is guaranteed, and sustainable utilization of the atractylodes macrocephala is achieved; meanwhile, efficient synthesis of secondary metabolites such as atractylone is facilitated, and the production efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of adventitious root culture technology, and more specifically to an airlift bioreactor for the culture of adventitious roots of Atractylodes macrocephala. Background Technology

[0002] Currently, due to the dwindling wild resources, many medicinal plants are being artificially cultivated. However, in field cultivation, plant growth is limited by climate, geography, and season. Furthermore, the use of pesticides and fertilizers in cultivation management can easily lead to the residue of harmful substances. At the same time, the unstable content of effective substances and the competition for land between medicinal materials and crops are also problems that hinder the development of my country's traditional Chinese medicine industry.

[0003] Adventitious roots expand the plant's absorption area and enhance its anchorage and support; therefore, the biological study of adventitious root formation is an important field of developmental biology. Furthermore, adventitious roots not only help elucidate the mechanisms regulating plant growth and development but also promote the vegetative propagation of many superior varieties, possessing immeasurable value for the development of agriculture, forestry, and horticulture. In tissue culture, the induction of adventitious roots is often used to obtain complete regenerated plants.

[0004] Using tissue culture instead of the original plant as the drug source, a large number of rapidly growing adventitious roots can be directly induced from the leaves and then cultured in suspension to obtain a culture containing effective chemical components. This is one of the effective ways to achieve the sustainable utilization of Atractylodes macrocephala. Therefore, how to provide a bioreactor that is conducive to the growth of adventitious roots of Atractylodes macrocephala is a problem that needs to be solved by those skilled in the art. Utility Model Content

[0005] In view of this, the present invention provides an airlift bioreactor for the cultivation of adventitious roots of Atractylodes macrocephala, which can obtain a large quantity of rapidly growing adventitious roots, realize the sustainable utilization of Atractylodes macrocephala, improve production efficiency, and reduce production costs. To achieve the above objectives, the present invention adopts the following technical solution:

[0006] This invention provides an airlift bioreactor for the culture of adventitious roots of Atractylodes macrocephala, comprising:

[0007] Support structure;

[0008] The reactor body is pre-installed on the support structure. The upper end of the reactor body is provided with a culture medium inlet, and a sealing element for sealing the reactor body is connected to the culture medium inlet. The lower end of the reactor body is provided with an air inlet, and an air inlet pipe is connected to the air inlet.

[0009] A conduit, one end of which is connected to the air inlet pipe, and the other end of which is connected to an oxygen pump, which delivers oxygen into the reactor body.

[0010] Furthermore, a filter is provided on the conduit to filter the gas introduced into the reactor body.

[0011] Furthermore, a sieve plate is provided at the air inlet of the reactor body.

[0012] Furthermore, a guide pipe is also provided at the air inlet, and the end of the guide pipe away from the air inlet is sealed.

[0013] As can be seen from the above technical solution, compared with the prior art, this utility model discloses an airlift bioreactor for the cultivation of adventitious roots of Atractylodes macrocephala. The reactor body is supported by a support structure. During the cultivation process, oxygen is introduced into the reactor body through an oxygen pump to ensure the rapid growth of adventitious roots of Atractylodes macrocephala, realize the sustainable utilization of Atractylodes macrocephala, and at the same time facilitate the efficient synthesis of secondary metabolites such as atractylone, improve production efficiency, and reduce production costs. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the airlift bioreactor for the cultivation of adventitious roots of Atractylodes macrocephala provided by this utility model.

[0016] In the diagram: 1. Culture medium inlet; 2. Reactor body; 3. Support structure; 4. Sieve plate; 5. Air inlet pipe; 6. Conduit; 7. Filter; 8. Flow guide pipe. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] See Figure 1 This utility model discloses an airlift bioreactor for the culture of adventitious roots of Atractylodes macrocephala, including...

[0019] Support structure 3;

[0020] The reactor body 2 is pre-installed on the support structure 3. The upper end of the reactor body 2 is provided with a culture medium inlet 1, and a sealing element for sealing the reactor body 2 is connected to the culture medium inlet 1. The lower end of the reactor body 2 is provided with an air inlet, and an air inlet pipe 5 is connected to the air inlet.

[0021] The conduit 6 is connected at one end to the air inlet pipe 5 and at the other end to an oxygen pump, which delivers oxygen into the reactor body 2.

[0022] The reactor body 2 is supported by the support structure 3 to ensure the stability of the reactor body 2 during the cultivation process.

[0023] In the culture of Atractylodes macrocephala adventitious roots, the roots are placed in a solid culture medium containing 1 / 2 MS + 0.5 mg / L IBA + 0.5 mg / L NAA + 30 g / L sucrose + 7.6 g / L agar, pH 5.8–6.0 for mass propagation. The propagated roots are then processed to a predetermined length. A suitable amount of the cut roots is loaded into reactor body 2. 1 / 2 MS liquid culture medium containing 0.5 mg / L IBA, 0.5 mg / L NAA, and 30 g / L sucrose is poured into reactor body 2, allowing the roots to propagate in the liquid medium. An oxygen pump introduces oxygen into reactor body 2 through conduit 6 via the air inlet, providing oxygen for the propagation of the roots and ensuring a suitable culture environment, thus guaranteeing successful cultivation.

[0024] The adventitious roots of Atractylodes macrocephala were placed in reactor body 2 containing liquid culture medium for cultivation to avoid browning and low proliferation coefficient during the cultivation process.

[0025] In some embodiments, a filter 7 is provided on the conduit 6 to filter the gas introduced into the reactor body 2.

[0026] The oxygen entering the reactor body 2 is filtered by filter 7 to ensure the cleanliness of the oxygen entering the reactor body 2, thereby ensuring the smooth progress of Atractylodes macrocephala adventitious root culture and the rapid propagation of Atractylodes macrocephala adventitious roots.

[0027] In some embodiments, a sieve plate 4 is provided at the air inlet of the reactor body 2.

[0028] In some embodiments, a guide pipe 8 is also provided at the air inlet, and the end of the guide pipe 8 away from the air inlet is sealed.

[0029] During the cultivation process, the guide pipe 8 is sealed to prevent the liquid inside the reactor body 2 from flowing out and affecting the cultivation of Atractylodes macrocephala adventitious roots;

[0030] After the adventitious roots of Atractylodes macrocephala are cultured, the liquid in the reactor body 2 can be discharged through the guide pipe 8, and the adventitious roots of Atractylodes macrocephala are prevented from entering the guide pipe 8 by the setting of the sieve plate.

[0031] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. For the apparatus disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple; relevant parts can be referred to the method section.

[0032] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An airlift bioreactor for the culture of adventitious roots of Atractylodes macrocephala, characterized in that, include: Support structure; The reactor body is pre-installed on the support structure. The upper end of the reactor body is provided with a culture medium inlet, and a sealing element for sealing the reactor body is connected to the culture medium inlet. The lower end of the reactor body is provided with an air inlet, and an air inlet pipe is connected to the air inlet. A conduit, one end of which is connected to the air inlet pipe, and the other end of which is connected to an oxygen pump, which delivers oxygen into the reactor body.

2. The airlift bioreactor for adventitious root culture of Atractylodes macrocephala according to claim 1, characterized in that, The duct is equipped with a filter to filter the gas introduced into the reactor body.

3. The airlift bioreactor for adventitious root culture of Atractylodes macrocephala according to claim 1, characterized in that, A sieve plate is installed at the air inlet of the reactor body.

4. The airlift bioreactor for adventitious root culture of Atractylodes macrocephala according to claim 1, characterized in that, A guide pipe is also provided at the air inlet, and the end of the guide pipe away from the air inlet is sealed.

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