Exosome extraction affinity capture device

Abstract

The utility model relates to an exosome extraction affinity capture device, which belongs to the technical field of exosome extraction, and comprises three vertically placed extraction boxes, the lower surfaces of the two extraction boxes at the top are provided with first groove bodies, the upper surfaces of the two extraction boxes at the bottom are in threaded connection with connecting blocks, and the first groove bodies are provided with second groove bodies. The first groove body is in threaded connection with the connecting block, an exosome affinity capture device is arranged in an inner cavity of each extraction box, and a connecting mechanism is arranged between every two adjacent extraction boxes. According to the exosome extraction affinity capture device, through mutual cooperation of the connecting mechanism and the protection mechanism, the connecting pipes at the upper end and the lower end can be separated, and the exosome affinity capture device is taken out of the extraction box; therefore, a worker can conveniently clean the inner cavity of the extraction box and the exosome affinity capture device after capturing the exosome in the sample, the exosome is prevented from being mixed with the residual exosome or waste liquid during reuse, and the exosome extraction purity is further improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to exosome extraction technical field, concretely is a kind of exosome extraction affinity capture device. BACKGROUND

[0002] Exosome is a kind of extracellular vesicle that cell secretes, and its main component is phospholipid bilayer and carries relevant content, so not all cell-secreted vesicles can be called exosome, and exosome extraction can be extracted from various body fluids, such as human blood, urine, semen and saliva, and also can be extracted from various cell cultures.

[0003] Through retrieval, it is found that Chinese patent publication No.CN218202738U discloses an exosome extraction device and an exosome affinity capture device, wherein the exosome extraction device includes a main unit, which is provided with a processing cavity, the processing cavity is provided with an original sample inlet and a waste liquid outlet, and the processing cavity is suitable for storing an original sample to be extracted exosome;Exosome affinity capture device, which is provided with exosome capture holes with uniform size suitable for capturing the exosome, is arranged inside the processing cavity and located between the original sample inlet and the waste liquid outlet;Drive unit, matched with the processing cavity, is suitable for pushing the original sample to flow through the exosome affinity capture device, the exosome extraction device provided by the application embodiment can improve the portability of the exosome extraction device and realize the direct extraction of exosome in the original sample.

[0004] Although the above-mentioned utility model can improve the convenience of exosome extraction, after exosome extraction, exosome, protein and other biomolecules may be left, and the main unit is not convenient for cleaning the inner cavity and exosome affinity capture device, so that the extracted exosome is mixed with the remaining exosome during the next extraction, thereby reducing the purity of exosome extraction. UTILITY MODEL CONTENT

[0005] In view of the deficiencies of the prior art, the utility model provides an exosome extraction affinity capture device, which has the advantages of easy cleaning, solves the problem that after exosome extraction, exosome, protein and other biomolecules may be left, and the main unit is not convenient for cleaning the inner cavity and exosome affinity capture device, so that the extracted exosome is mixed with the remaining exosome during the next extraction, thereby reducing the purity of exosome extraction.

[0006] To achieve the above object, the utility model provides following technical scheme: an exosome extraction affinity capture device, including three vertical placement's extraction box, the lower surface of two extraction boxes at the top is equipped with a groove, the upper surface of two extraction boxes at the bottom is screw -threaded with the connecting block, it is screw -threaded between the groove and the connecting block, the inner chamber of extraction box is equipped with exosome affinity capture device, the same connecting mechanism for liquid flow is provided between two adjacent extraction boxes, the lower surface of exosome affinity capture device is equipped with protection mechanism,

[0007] The connecting mechanism includes two connecting pipes communicated between adjacent two extraction boxes, the outer side of the bottom of the upper end connecting pipe is fixed with a sleeve, the outer surface of the top of the lower end connecting pipe is fixed with an annular plate, the left and right ends of the inner chamber of the annular plate are both equipped with a placing cavity, the inner wall of the side opposite to the left and right ends of the placing cavity is both fixed with a return spring, the side opposite to the left and right ends of the return spring is both fixed with a poking plate, the upper surface of two poking plates is both penetrated through the inner top wall of the annular plate and extends to the upper side of the annular plate, and the top of the side opposite to the two poking plates is both fixed with a clamping block, the bottom of the left and right sides of the sleeve is both equipped with a clamping groove.

[0008] Further, the bottom of the right side of the extraction box is communicated with a waste liquid pipe, the upper surface of the exosome affinity capture device is equipped with a plurality of evenly distributed exosome capture holes, and the outer diameter of the exosome affinity capture device is matched with the inner diameter of the extraction box.

[0009] Further, one end of the extraction box at the top is communicated with the middle part of the inner chamber of the extraction box at the top, the connecting pipe at the top is communicated with the top of the inner chamber of the extraction box at the top, the connecting pipe at the bottom is communicated with the middle part of the inner chamber of the extraction box at the top, and the connecting pipe at the bottom is communicated with the top of the inner chamber of the extraction box at the bottom.

[0010] Further, the inner diameter of the sleeve is matched with the outer diameter of the connecting pipe, and the size of the inner chamber of the clamping groove is matched with the size of the clamping block.

[0011] Further, the left and right ends of the inner bottom wall of the annular plate are both equipped with a limiting groove, the lower surface of the poking plate is fixed with a limiting block, the limiting block moves horizontally in the inner chamber of the limiting groove, and the cross section shape of the poking plate is inverted L-shaped.

[0012] Further, the protection mechanism includes a screwing block fixed on the lower surface of the exosome affinity capture device, the outer surface of the screwing block is screw -threaded with a mounting block, the top of the left and right sides of the mounting block is both fixed with a flow guide plate, the middle part of the inner bottom wall of the extraction box is fixed with a protection box, the inner bottom wall of the protection box is fixed with a return spring, and the top of the return spring is fixed with the mounting block.

[0013] Further, the included angle between the guide plate and the left and right side walls of the mounting block is an acute angle, and the outer diameter of the mounting block is smaller than the inner diameter of the protective box.

[0014] Compared with the prior art, the technical scheme has the following beneficial effects:

[0015] The exosome extraction affinity capture device can separate the connecting pipes at the upper end and the lower end and take out the exosome affinity capture device from the extraction box through the cooperation between the connecting mechanism and the protection mechanism, so that the inner cavity of the extraction box and the exosome affinity capture device can be cleaned after the exosomes in the sample are captured, the exosomes or waste liquid remaining in the extraction box are prevented from being mixed again, and the purity of the extracted exosomes is improved. BRIEF DESCRIPTION OF DRAWINGS

[0016] Fig. 1 It is a structural schematic view of the utility model;

[0017] Fig. 2 It is a structural schematic view of the exosome affinity capture device of the utility model;

[0018] Fig. 3 It is a structural schematic view of the connecting mechanism of the utility model;

[0019] Fig. 4 It is an enlarged schematic view of the structure at A of the utility model

[0020] Fig. 5 It is a structural schematic view of the protection mechanism of the utility model.

[0021] In the figure: 1 extraction box, 2 No. 1 groove body, 3 connecting block, 4 exosome affinity capture device, 5 connecting mechanism, 501 connecting pipe, 502 sleeve, 503 annular plate, 504 placing cavity, 505 return spring, 506 push plate, 507 clamping block, 508 clamping groove, 6 protection mechanism, 601 screw-in block, 602 mounting block, 603 guide plate, 604 protective box, 605 return spring. DETAILED DESCRIPTION

[0022] The technical scheme in the embodiments of the utility model will be described clearly and completely below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the utility model.

[0023] Please refer to Figs. 1-2The exosome extraction affinity capture device in the embodiment comprises three vertically placed extraction boxes 1, the lower surfaces of the top two extraction boxes 1 are each provided with a No. 1 groove 2, the upper surfaces of the bottom two extraction boxes 1 are each threadedly connected with a connecting block 3, the No. 1 groove 2 is threadedly connected between the connecting block 3, the inner cavity of the extraction box 1 is provided with an exosome affinity capture device 4, the same connecting mechanism 5 for liquid circulation is arranged between the adjacent two extraction boxes 1, and the lower surface of the exosome affinity capture device 4 is provided with a protection mechanism 6.

[0024] The bottom of the right side of the extraction box 1 is communicated with a waste liquid pipe, the upper surface of the exosome affinity capture device 4 is provided with a plurality of evenly distributed exosome capture holes, and the outer diameter of the exosome affinity capture device 4 is matched with the inner diameter of the extraction box 1.

[0025] It can be understood that the sample is poured into the inner cavity of the topmost extraction box 1, at this time the exosome affinity capture device 4 can perform affinity capture on the exosomes in the sample, and the other waste liquid part passes through the exosome affinity capture device 4, enters the bottom of the inner cavity of the extraction box 1 under the action of the left and right two side flow guides 603 and then flows out through the waste liquid pipe.

[0026] Please refer to Figs. 3-4 In order to facilitate the separation of the two extraction boxes 1, the connecting mechanism 5 in the embodiment comprises two connecting pipes 501 communicated between the adjacent two extraction boxes 1, the outer side of the bottom of the upper end connecting pipe 501 is fixedly provided with a sleeve 502, the outer surface of the top of the lower end connecting pipe 501 is fixedly provided with an annular plate 503, the left and right two ends of the inner cavity of the annular plate 503 are each provided with a placing cavity 504, the inner walls of the left and right two ends of the placing cavity 504 away from each other are each fixedly provided with a return spring 505, the sides away from each other of the left and right two ends of the return spring 505 are each fixedly provided with a push plate 506, the upper surfaces of the two push plates 506 are each penetrated through the inner top wall of the annular plate 503 and extend to the upper side of the annular plate 503 and the top of the side away from each other of the two push plates 506 is each fixedly provided with a clamping block 507, the bottoms of the left and right two sides of the sleeve 502 are each provided with a clamping groove 508, by moving the left and right two push plates 506 away from the sleeve 502, the movement of the push plate 506 drives the movement of the clamping block 507, at this time the return spring 505 is stretched, until the clamping block 507 moves out of the inner cavity of the clamping groove 508, the lower end connecting pipe 501 in the sleeve 502 is pulled down, so that the lower end connecting pipe 501 drives the annular plate 503 to move downward, then the limiting of the push plate 506 is released, so that the two connecting pipes 501 can be separated.

[0027] The topmost extraction box 1 one end of the connecting pipe 501 is communicated with the middle part of the inner cavity of the topmost extraction box 1, the middle top connecting pipe 501 is communicated with the top of the inner cavity of the middle top extraction box 1, the middle bottom connecting pipe 501 is communicated with the middle part of the inner cavity of the middle top extraction box 1, and the bottommost connecting pipe 501 is communicated with the top of the inner cavity of the bottommost extraction box 1.

[0028] In the embodiment, the inner diameter of the sleeve 502 is matched with the outer diameter of the connecting pipe 501, the size of the inner cavity of the clamping groove 508 is matched with the size of the clamping block 507, the left and right ends of the inner bottom wall of the annular plate 503 are provided with limiting grooves, the lower surface of the driving plate 506 is fixed with a limiting block, the limiting block moves horizontally in the inner cavity of the limiting groove, and the cross section shape of the driving plate 506 is inverted L-shaped.

[0029] Please refer to Fig. 5 In order to protect the reset spring 605, the protection mechanism 6 in the embodiment comprises a screwing block 601 fixed on the lower surface of the exosome affinity capture device 4, the outer surface of the screwing block 601 is threadedly connected with a mounting block 602, the top of the left and right sides of the mounting block 602 is fixed with a flow guide plate 603, the middle part of the inner bottom wall of the extraction box 1 is fixed with a protection box 604, the protection box 604 is arranged to avoid the contact between the waste liquid and the reset spring 605, thereby protecting the service life of the reset spring 605, the inner bottom wall of the protection box 604 is fixed with the reset spring 605, the top of the reset spring 605 is fixed with the mounting block 602, as the sample increases, the exosome affinity capture device 4 is pressed downward by the gravity of the sample, thereby the reset spring 605 is pressed and shortened, so that when the exosome affinity capture device 4 moves downward to the connecting pipe 501, the sample and the substance blocked by the exosome affinity capture device 4 enter the inner cavities of the two extraction boxes 1 below through the connecting pipes 501 at the two ends to capture the exosomes by the exosome affinity capture device 4.

[0030] The included angle between the flow guide plate 603 and the left and right side walls of the mounting block 602 is an acute angle, and the outer diameter of the mounting block 602 is smaller than the inner diameter of the protection box 604.

[0031] It can be understood that rotating the exosome affinity capture device 4 can drive the screwing block 601 to separate from the mounting block 602, thereby the exosome affinity capture device 4 can be taken out from the inner cavity of the extraction box 1, the collected exosomes are processed, and the inner cavity of the extraction box 1 and the collected exosome affinity capture device 4 are cleaned.

[0032] It should be noted that the connecting pipe 501 at the top and bottom is separated, and the exosome affinity capture device 4 is taken out of the extraction box 1, so that the staff can clean the inner cavity of the extraction box 1 and the exosome affinity capture device 4, prevent mixing with residual exosomes or waste liquid when reused, and thus improve the purity of exosome extraction.

[0033] The working principle of the above embodiment is:

[0034] (1) When extracting exosomes, the sample can be poured into the inner cavity of the top extraction box 1. At this time, the exosome affinity capture device 4 can capture the exosomes in the sample, and the other waste liquid part passes through the exosome affinity capture device 4 and enters the bottom of the inner cavity of the extraction box 1 under the action of the left and right two side flow guides 603, and then flows out through the waste liquid pipe. As the sample increases, the exosome affinity capture device 4 is pressed by the gravity of the sample, and can press the rotating block 601 and the mounting block 602 downward, thereby causing the reset spring 605 to be pressed and shortened, so that the exosome affinity capture device 4 moves downward to the connecting pipe 501, and the sample and the substance blocked by the exosome affinity capture device 4 enter the inner cavities of the two extraction boxes 1 below through the connecting pipe 501 at the top and bottom. The exosome affinity capture device 4 is used for exosome capture. The protective box 604 can avoid the contact between the waste liquid and the reset spring 605, and protects the service life of the reset spring 605.

[0035] (2) After the exosomes are captured, in order to facilitate the cleaning of the inner cavity of the extraction box 1, the left and right two side plates 506 can be moved away from the sleeve 502. The movement of the plate 506 drives the movement of the block 507. At this time, the return spring 505 is stretched until the block 507 moves out of the inner cavity of the slot 508. The connecting pipe 501 at the lower end in the sleeve 502 can be pulled downward, so that the connecting pipe 501 at the lower end drives the annular plate 503 to move downward. Then release the limit of the plate 506, so that the two connecting pipes 501 are separated, the first groove body 2 is separated from the connecting block 3, the adjacent two extraction boxes 1 are separated, then the connecting block 3 is rotated from the extraction box 1, and the exosome affinity capture device 4 is rotated, which can drive the rotating block 601 and the mounting block 602 to separate, and then the exosome affinity capture device 4 can be taken out from the inner cavity of the extraction box 1. The exosomes are collected and processed, and the collected exosome affinity capture device 4 and the inner cavity of the extraction box 1 are cleaned.

[0036] It is to be noted that, in the present document, relational terms such as first and second and the like can be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises", "comprising", or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus. An element proceeded by "comprises... a" does not, without more constraints, exclude the presence of additional identical elements in the process, method, article, or apparatus that comprises the element.

[0037] While the embodiments of the present application have been illustrated and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the spirit and scope of the application.

Claims

1. An exosome extraction affinity capture device, comprising three vertically arranged extraction boxes (1), characterized in that: The two extraction boxes (1) at the top each have a groove (2) on their lower surface, and the two extraction boxes (1) at the bottom each have a connecting block (3) threadedly connected to their upper surface. The groove (2) and the connecting block (3) are threadedly connected. The inner cavity of the extraction box (1) is provided with an exosome affinity capture device (4). A connecting mechanism (5) for liquid flow is provided between two adjacent extraction boxes (1). The lower surface of the exosome affinity capture device (4) is provided with a protective mechanism (6). The connecting mechanism (5) includes two connecting tubes (501) connecting two adjacent extraction boxes (1). A sleeve (502) is fixed to the outer side of the bottom of the upper connecting tube (501). An annular plate (503) is fixed to the outer surface of the top of the lower connecting tube (501). Placement cavities (504) are opened at both ends of the inner cavity of the annular plate (503). A return spring (505) is fixed to the inner wall of the opposite side of the placement cavity (504) at both ends. A toggle plate (506) is fixed to the opposite side of the return spring (505) at both ends. The upper surfaces of the two toggle plates (506) penetrate the inner top wall of the annular plate (503) and extend to the upper side of the annular plate (503). A locking block (507) is fixed to the top of the opposite side of the two toggle plates (506). A locking groove (508) is opened at the bottom of both sides of the sleeve (502).

2. The exosome extraction affinity capture device according to claim 1, characterized in that: Waste liquid pipes are connected to the bottom right side of the extraction box (1). Several uniformly distributed exosome capture holes are opened on the upper surface of the exosome affinity capture device (4). The outer diameter of the exosome affinity capture device (4) is adapted to the inner diameter of the extraction box (1).

3. The exosome extraction affinity capture device according to claim 1, characterized in that: The connecting tube (501) at one end of the topmost extraction box (1) is connected to the middle of the inner cavity of the topmost extraction box (1), the connecting tube (501) at the top middle is connected to the top of the inner cavity of the top middle extraction box (1), the connecting tube (501) at the bottom middle is connected to the middle of the inner cavity of the top middle extraction box (1), and the connecting tube (501) at the bottom bottom is connected to the top of the inner cavity of the bottommost extraction box (1).

4. The exosome extraction affinity capture device according to claim 1, characterized in that: The inner diameter of the sleeve (502) is adapted to the outer diameter of the connecting pipe (501), and the size of the inner cavity of the slot (508) is adapted to the size of the block (507).

5. The exosome extraction affinity capture device according to claim 1, characterized in that: Limiting grooves are provided at both ends of the bottom wall of the annular plate (503). A limiting block is fixed on the lower surface of the actuating plate (506). The limiting block moves horizontally in the inner cavity of the limiting groove. The cross-sectional shape of the actuating plate (506) is an inverted L shape.

6. The exosome extraction affinity capture device according to claim 1, characterized in that: The protective mechanism (6) includes a screw-in block (601) fixed to the lower surface of the exosome affinity capture device (4). The outer surface of the screw-in block (601) is threaded with a mounting block (602). The top of the left and right sides of the mounting block (602) is fixed with a guide plate (603). The middle of the inner bottom wall of the extraction box (1) is fixed with a protective box (604). The inner bottom wall of the protective box (604) is fixed with a return spring (605). The top of the return spring (605) is fixed with the mounting block (602).

7. The exosome extraction affinity capture device according to claim 6, characterized in that: The angle between the guide plate (603) and the left and right side walls of the mounting block (602) is an acute angle, and the outer diameter of the mounting block (602) is smaller than the inner diameter of the protective box (604).

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