Detection device for cell preparation

Abstract

The utility model relates to the technical field of cell preparation, in particular to a detection device for cell preparation, which comprises a detection table mounted on a base, a centrifugal separation mechanism for centrifugally separating cells in a centrifugal tube is arranged on the base, and a dyeing mechanism for dyeing the cells is arranged on the detection table. The centrifugal motor drives the placement base to rotate, so that the centrifugal tube in the placement base is driven to quickly rotate, cells and a culture medium are completely separated by the generated centrifugal force, the separation efficiency is higher, the cells are more conveniently sampled and detected, and the driving motor drives the rotating shaft to rotate, so that the rubber ball is driven to rotate to impact the detection table. The glass slide on the detection table is driven to vibrate through impact, so that rendering of cells by a staining agent in the glass slide is accelerated, the glass slide is clamped and fixed through cooperation of elasticity of an extension spring and a positioning sliding block, and the situation that the cells in the glass slide fall off due to vibration overturning of the glass slide is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell preparation technical field especially, it relates to a detection device for cell preparation. BACKGROUND

[0002] Cell preparation refers to extracting, isolating, culturing and amplifying specific types of cells from biological organisms through a series of technical means to meet the needs of scientific research, drug development or clinical treatment. Cell preparation is of great significance in biomedical research. It not only helps researchers to understand the biological characteristics of cells in depth, but also provides new methods and strategies for disease treatment.

[0003] When preparing cells, the cell culture situation needs to be detected. At this time, the cells need to be separated from the culture medium or other liquids for detection. The traditional way is for the detection personnel to manually shake and separate the cells, which is very labor-intensive, and the cell separation is not thorough, which is not conducive to the subsequent detection work. UTILITY MODEL CONTENT

[0004] In view of the deficiencies of the prior art, the utility model provides a detection device for cell preparation, which solves the technical problems of manual shaking and separating cells and culture medium, which is very labor-intensive, and the cell separation is not thorough, and achieves the purpose of quickly separating cells and culture medium.

[0005] To solve the above technical problems, the utility model provides the following technical scheme: a detection device for cell preparation, comprising a detection table installed on a base, the base is provided with a centrifugal separation mechanism for centrifugal separation of cells in a centrifugal tube, and the detection table is provided with a staining mechanism for staining cells.

[0006] The centrifugal separation mechanism comprises a placing base installed in a hole slot formed in the center of the detection table, a limiting frame is installed on the placing base, a rotating shaft is installed at the bottom of the placing base, and a centrifugal motor is installed in the base to drive the rotating shaft to rotate.

[0007] Preferably, the staining mechanism comprises a glass slide placed on the detection table, a spring slot is symmetrically formed with the center line of the detection table on one side of the detection table, and a sliding groove is formed at the end of the spring slot, a tension spring is installed on the inner wall of the spring slot, the other end of the tension spring is installed with a positioning sliding block slidingly connected in the sliding groove, a staining assembly for staining cells in the glass slide is installed at the rear end of the base, and a vibration assembly for vibrating the glass slide on the detection table is installed at the four corners of the base.

[0008] Preferably, the dyeing assembly comprises a dyeing barrel mounted at the rear end of the base through the support frame, a dyeing dropper is mounted at the bottom of the dyeing barrel, and an electric valve is mounted on the dyeing dropper.

[0009] Preferably, a liquid injection bucket is mounted at the top of the dyeing barrel, and an observation window made of transparent tempered glass is mounted on the front of the dyeing barrel.

[0010] Preferably, an annular groove is formed at the top of the inner wall of the base, rotating sliding blocks slidably connected in the annular groove are mounted at the four corners of the detection table, and a handle is mounted at one side of the top of the detection table.

[0011] Preferably, the vibration assembly comprises a driving motor mounted on the inner wall of the base, a rotating shaft is mounted at the output end of the driving motor, a flexible spring is mounted on the rotating shaft, and a rubber ball is mounted at the other end of the flexible spring.

[0012] By the above technical scheme, the detection device for cell preparation provided by the present application has at least the following beneficial effects:

[0013] 1. The centrifugal motor drives the placement base to rotate, thereby driving the centrifugal tube in it to rotate quickly, the centrifugal force generated separates the cells and the culture medium completely, the separation efficiency is higher, and it is more convenient to sample and detect the cells.

[0014] 2. The driving motor drives the rotating shaft to rotate, thereby driving the rubber ball to rotate and impact the detection table, the slide glass on the detection table is vibrated through the impact, thereby accelerating the rendering of the dyeing agent in the slide glass to the cells.

[0015] 3. The slide glass is clamped and fixed by the elastic positioning sliding block of the extension spring, so that the cells in the slide glass are prevented from falling due to vibration and overturning. BRIEF DESCRIPTION OF DRAWINGS

[0016] The drawings described herein are used to provide further understanding of the present application, constitute a part of the present application, the schematic embodiments of the present application and the description thereof are used to explain the present application, and do not constitute improper limitation on the present application.

[0017] In the drawings:

[0018] Figure 1 It is a whole structure schematic view of the present application;

[0019] Figure 2 It is an independent cross-sectional structure schematic view of the centrifugal separation mechanism of the present application;

[0020] Figure 3 It is an independent cross-sectional structure schematic view of the dyeing mechanism of the present application;

[0021] Figure 4 It is detection table and base split structure schematic view of the utility model;

[0022] Figure 5 It is base section view internal structure schematic view of the utility model.

[0023] In the figure: 1, base;2, detection table;3, centrifugal separation mechanism;301, place base;302, limit support;303, rotation shaft;304, centrifugal motor;4, dyeing mechanism;401, glass slide;402, elastic slot;403, sliding slot;404, tension spring;405, positioning sliding block;406, dyeing assembly;4061, dyeing barrel;4062, dyeing dropper;4063, electric valve;4064, liquid injection bucket;4065, observation window;4066, annular groove;4067, rotating sliding block;4068, handle;407, vibration assembly;4071, drive motor;4072, rotating shaft;4073, flexible spring;4074, rubber ball. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0025] Embodiment 1

[0026] Based on the problems that the existing manual shaking separation of cells and culture medium is very labor-consuming, and the cell separation is not thorough enough, the embodiment provides a detection device for cell preparation, please refer to Figures 1-5 The embodiment provides a detection device for cell preparation, which can quickly separate cells and culture medium. The detection device for cell preparation comprises a detection table 2 installed on a base 1, the base 1 is provided with a centrifugal separation mechanism 3 for centrifugal separation of cells in a centrifugal tube, the detection table 2 is provided with a dyeing mechanism 4 for dyeing cells, the centrifugal separation mechanism 3 is used for separating cells and culture medium in the centrifugal tube, which is convenient for subsequent detection of cells, and the dyeing mechanism 4 is used for quickly dyeing cells to be detected.

[0027] Since the prior art manual shaking separation of cells and culture medium is very laborious, and the cell separation is not thorough enough, the device is provided with a centrifugal separation mechanism 3, which includes a placing base 301 installed in the hole groove opened in the center of the detection table 2, a limiting frame 302 is installed on the placing base 301, a rotating shaft 303 is installed at the bottom of the placing base 301, a centrifugal motor 304 is installed in the base 1 to drive the rotating shaft 303 to rotate, the centrifugal tube is inserted into the placing base 301, and the centrifugal tube is limited and fixed by the limiting frame 302, then the centrifugal motor 304 is started to drive the rotating shaft 303 to rotate, and then the placing base 301 is driven to rotate, so that the centrifugal tube in it is rapidly rotated, and the centrifugal force generated separates the cells and the culture medium completely, which is more convenient for sampling and detecting the cells.

[0028] After the cells are extracted, they need to be placed on the slide 401 for detection and observation under a microscope. In order to prevent the slide 401 from sliding, the device is provided with a staining mechanism 4, which includes the slide 401 placed on the detection table 2, the detection table 2 is symmetrically provided with an elastic groove 402 at the center line of the detection table 2, and the end of the elastic groove 402 is extended to form a sliding groove 403, a tension spring 404 is installed on the inner wall of the elastic groove 402, the other end of the tension spring 404 is installed with a positioning sliding block 405 slidingly connected in the sliding groove 403, a staining assembly 406 is installed at the rear end of the base 1 to stain the cells in the slide 401, and vibration assemblies 407 are installed at the four corners of the base 1 to vibrate the slide 401 on the detection table 2. Pull the positioning sliding block 405 of the arc structure to slide backward along the sliding groove 403 and stretch the tension spring 404 in the elastic groove 402, then place the slide 401 on the inside of the positioning sliding block 405, then release the positioning sliding block 405, and use the elasticity of the tension spring 404 to clamp the slide 401 between the two positioning sliding blocks 405, so as to avoid the shaking of the slide 401 affecting the detection and observation.

[0029] In order to better observe the condition of the cells, the cells need to be stained, so the device is also provided with a staining assembly 406, which includes a staining barrel 4061 installed at the rear end of the base 1 through a support frame, a staining dropper 4062 is installed at the bottom of the staining barrel 4061, an electric valve 4063 is installed on the staining dropper 4062, the electric valve 4063 is started, the staining agent in the staining barrel 4061 is dropped into the slide 401 through the staining dropper 4062, and the cells in the slide 401 are stained, which is convenient for subsequent observation and detection.

[0030] The top of the dyeing tank 4061 is equipped with a liquid injection funnel 4064, and the front of the dyeing tank 4061 is equipped with an observation window 4065 made of transparent tempered glass. The remaining amount of dye in the dyeing tank 4061 can be observed in real time through the observation window 4065, and replenished in time through the liquid injection funnel 4064.

[0031] To facilitate staining and observation, the device has an annular groove 4066 on the top of the inner wall of the base 1. Rotating sliders 4067, which are slidably connected to the annular groove 4066, are installed at the four corners of the detection stage 2. A handle 4068 is installed on one side of the top of the detection stage 2. Pulling the handle 4068 will cause the detection stage 2 to rotate in the annular groove 4066 in the base 1 through the rotating sliders 4067 installed on its outer side, thereby causing the slides 401 on the detection stage 2 to move and stain all the slides 401.

[0032] Example 2

[0033] Based on Example 1, such as Figures 1-5 As shown, since a certain amount of time is needed for the staining agent to fully spread after it is dropped onto the slide 401, the device is also equipped with a vibration component 407 to further improve the staining efficiency. The vibration component 407 includes a drive motor 4071 installed on the inner wall of the base 1. A rotating shaft 4072 is installed at the output end of the drive motor 4071. A flexible spring 4073 is installed on the rotating shaft 4072. A rubber ball 4074 is installed at the other end of the flexible spring 4073. When the staining agent is dropped into the slide 401, the drive motor 4071 is started to drive the rotating shaft 4072 to rotate, which in turn drives the rubber ball 4074 at the other end of the flexible spring 4073 on the rotating shaft 4072 to rotate and impact the detection stage 2. The impact causes the slide 401 on the detection stage 2 to vibrate, thereby accelerating the staining of the staining agent.

[0034] It should be noted that, in this document, the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0035] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A detection device for cell preparation, comprising a detection stage (2) mounted on a base (1), characterized in that: The base (1) is provided with a centrifugation separation mechanism (3) for centrifuging and separating cells in centrifuge tubes, and the detection platform (2) is provided with a staining mechanism (4) for staining cells; The centrifugal separation mechanism (3) includes a placement base (301) installed in a slot in the center of the detection table (2), a limit frame (302) installed on the placement base (301), a rotating shaft (303) installed at the bottom of the placement base (301), and a centrifugal motor (304) for driving the rotating shaft (303) to rotate is installed in the base (1).

2. The detection device for cell preparation according to claim 1, characterized in that: The staining mechanism (4) includes a glass slide (401) placed on the detection stage (2). An elastic groove (402) is symmetrically provided on one side of the detection stage (2) with respect to the center line of the detection stage (2). A sliding groove (403) extends from the end of the elastic groove (402). A tension spring (404) is installed on the inner wall of the elastic groove (402). A positioning slider (405) is slidably connected in the sliding groove (403) at the other end of the tension spring (404). A staining component (406) for staining cells in the glass slide (401) is installed at the rear end of the base (1). A vibration component (407) for vibrating the glass slide (401) on the detection stage (2) is installed at each of the four corners inside the base (1).

3. The detection device for cell preparation according to claim 2, characterized in that: The dyeing assembly (406) includes a dyeing tank (4061) mounted on the rear end of the base (1) via a support frame. A dyeing dropper (4062) is installed at the bottom of the dyeing tank (4061), and an electric valve (4063) is installed on the dyeing dropper (4062).

4. The detection device for cell preparation according to claim 3, characterized in that: The top of the staining tank (4061) is equipped with a liquid injection hopper (4064), and the front of the staining tank (4061) is equipped with an observation window (4065) made of transparent tempered glass.

5. The detection device for cell preparation according to claim 2, characterized in that: The base (1) has an annular groove (4066) on the top of its inner wall. The four corners of the testing platform (2) are equipped with rotating sliders (4067) that are slidably connected in the annular groove (4066). A handle (4068) is installed on one side of the top of the testing platform (2).

6. The detection device for cell preparation according to claim 2, characterized in that: The vibration assembly (407) includes a drive motor (4071) mounted on the inner wall of the base (1), a rotating shaft (4072) is mounted on the output end of the drive motor (4071), a flexible spring (4073) is mounted on the rotating shaft (4072), and a rubber ball (4074) is mounted on the other end of the flexible spring (4073).

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