Bioreactor

Abstract

The utility model relates to the field of medical equipment, in particular to a bioreactor which comprises a reaction bottle and a power assembly. The reaction bottle comprises a bottle body, a cover body matched with the bottle body, a rotating shaft and a plurality of stirring blades connected to the rotating shaft, the rotating shaft penetrates through the bottom of the bottle body and is rotationally connected with the bottle body, the rotating shaft is in sealed connection with the bottle body, and one end, located outside the bottle body, of the rotating shaft is an extension end of the rotating shaft. The power assembly comprises a shell and a stirring motor, the top of the shell is open, the extending end of the rotating shaft is connected to the stirring motor arranged in the shell, and the stirring motor is used for driving the rotating shaft to controllably rotate along the middle shaft. The stirring blades are arranged close to the inner wall of the bottom of the bottle body, so that upward liquid flow is continuously generated in the bottle body, organisms are driven to ascend and descend due to gravity after being far away from the stirring blades, the positions of the organisms are greatly changed, stirring dead angles at the bottom of the bottle body are reduced, and the culture effect is improved.

Description

Technical Field

[0001] This utility model relates to the field of medical devices, and in particular to a bioreactor. Background Technology

[0002] A bioreactor is a device system that utilizes the biological functions of enzymes or organisms to carry out biochemical reactions in vitro. It promotes the growth, metabolism, and product formation of biological systems by providing a suitable in vitro environment. Bioreactors optimize the growth of organisms and the production of products by controlling parameters such as temperature, pH, oxygen concentration, and nutrient concentration. To ensure that the organisms, i.e., cells or microorganisms, can fully grow and metabolize, continuous stirring is required to achieve uniform distribution of the organisms in the reaction solution, thereby ensuring that the nutrient concentration and other parameters of the environment in which each organism exists are at the target values.

[0003] Current bioreactors all use a rotating shaft to drive stirring blades for agitation. This method is effective for organisms close to the blades, but the agitation effect decreases with increasing distance. Current bioreactors typically employ side-wall or top-mounted stirring, which creates dead zones. To reduce these dead zones, existing bioreactors usually incorporate multiple sets of stirring blades at different heights. However, the organisms, constrained by the flow patterns created by these different blades, remain at similar heights over time, which is also detrimental to improving cultivation efficiency. Utility Model Content

[0004] The purpose of this invention is to provide a bioreactor with better stirring effect.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A bioreactor, comprising:

[0007] A reaction flask includes a flask body, a cap body fitted to the flask body, a rotating shaft, and a plurality of stirring blades connected to the rotating shaft. The rotating shaft passes through the bottom of the flask body and is rotatably connected to the flask body. The rotating shaft is sealed to the flask body. One end of the rotating shaft located outside the flask body is an extension end of the rotating shaft.

[0008] The power assembly includes a housing and a stirring motor. The housing has an opening at the top, and the extended end of the rotating shaft is connected to the stirring motor disposed inside the housing. The stirring motor is used to drive the rotating shaft to rotate controllably along a central axis.

[0009] Optionally, the power assembly further comprises a mounting seat arranged at the opening of the housing and connected to the stirring motor, and the extending end of the rotating shaft is detachably connected to the mounting seat.

[0010] Optionally, a middle portion of the top of the housing is provided with a protruding positioning portion, and a bottom of the reaction bottle is formed with a matching groove matched with the positioning portion, and the mounting seat is arranged at the top of the positioning portion.

[0011] Optionally, the positioning portion is integrally configured in a circular truncated cone shape, and a diameter of the top of the positioning portion is smaller than that of the bottom.

[0012] Optionally, a thickness of the bottom of the reaction bottle is greater than a depth of the matching groove.

[0013] Optionally, a plurality of clamping portions are protruded on an outer wall of the extending end of the rotating shaft, and the clamping portions are configured in a strip shape parallel to the rotating shaft.

[0014] Optionally, a plurality of the reaction bottles have different volumes, and each of the reaction bottles is matched with the power assembly.

[0015] Optionally, the bottle body has a plurality of inlets and outlets for communicating inside and outside of the bottle body, and a plurality of the cover bodies are respectively matched with the inlets and outlets.

[0016] Optionally, the power assembly further comprises a counterweight arranged at a position close to an edge of the bottom of the power assembly, for stabilizing a gravity center of the power assembly.

[0017] Optionally, the reaction bottle is configured by medical plastic.

[0018] The beneficial effects of the present application are as follows: the rotating shaft is connected to the bottom of the bottle body, so that the stirring blade is adjacent to the bottom of the bottle body, and when the stirring blade rotates with the rotating shaft, continuous upward liquid flow is generated, the organisms flow to the liquid surface under the driving of the liquid flow, and when rising to a height close to the liquid surface, the organisms sink due to the gravity away from the stirring blade, so that the continuous change of the position of the organisms is realized. This design also helps to reduce the stirring dead angle at the bottom of the bottle, prevents the organisms from sinking to the bottom, and helps the organisms to be uniformly distributed in the reaction liquid, so that the reaction is fully carried out.

[0019] The above description is only a summary of the technical scheme of the present application, in order to more clearly understand the technical means of the present application, and the content of the specification can be implemented, the following preferred embodiments of the present application are described in detail with reference to the drawings. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1The utility model discloses a structure diagram of the bioreactor shown in embodiment one of the utility model.

[0021] Figure 2 The utility model discloses a structure diagram of the bioreactor shown in embodiment two of the utility model.

[0022] Legend: 1-reaction bottle, 11-bottle body, 111-containing part, 112-inlet and outlet, 113-mating groove, 12-cover body, 13-rotation shaft, 131-extension end, 132-clamping part, 14-stirring blade, 15-connecting piece, 2-power assembly, 21-housing, 211-positioning part, 212-stabilizing part, 22-mounting seat, 221-clamping groove, 23-stirring motor, 24-counterweight. DETAILED DESCRIPTION

[0023] The technical scheme of the utility model will be described below in conjunction with the drawings, obviously, the described embodiment is a part of the embodiment of the utility model, not all the embodiment. Based on the embodiment in the utility model, all other embodiments obtained by the person skilled in the art without making the creative labor belong to the scope of the utility model protection.

[0024] In the description of the utility model, it is necessary to explain that the orientation or position relation of the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like is based on the orientation or position relation shown in the drawing, only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the indicated device or element must have a particular orientation, a particular orientation and operation, therefore, it can not be understood as the limitation of the utility model. In addition, the terms "first", "second", "third" are only for the purpose of description, and can not be understood as indicating or implying relative importance.

[0025] In the description of the utility model, it is necessary to explain that, unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connecting" should be understood broadly, for example, can be fixedly connected, can be detachably connected, or integrally connected, can be mechanically connected, or electrically connected, can be directly connected, or indirectly connected through the intermediate medium, can be the communication inside two elements. For the person skilled in the art, the specific meaning of the above-mentioned terms in the utility model can be understood according to the specific circumstances.

[0026] In addition, the technical features involved in different embodiments of the utility model described below can be combined with each other as long as there is no conflict.

[0027] Please see Figure 1The utility model application protection's bioreactor includes reaction bottle 1 and power assembly 2. Reaction bottle 1 includes bottle body 11, the cover 12 that cooperates in bottle body 11, rotating shaft 13 and a plurality of stirring vane 14 connected in rotating shaft 13, rotating shaft 13 passes through the bottom of bottle body 11, and with bottle body 11 rotation connection, and rotating shaft 13 and bottle body 11 sealed connection, rotating shaft 13 is located the extension end 131 of rotating shaft 13 in bottle body 11 outside one end. Power assembly 2 includes casing 21 and stirring motor 23, and casing 21 top opening, and the extension end 131 of rotating shaft 13 is connected in the stirring motor 23 of setting in casing 21 interior, and stirring motor 23 is used to drive rotating shaft 13 controllably along the central axis rotation.

[0028] Connect rotating shaft 13 with bottle body 11 bottom, make stirring vane 14 adjacent bottle body 11 bottom, when stirring vane 14 rotates with rotating shaft 13, continuously generate upward flow, and organism is driven to flow to liquid level under the flow, and when ascending to the height close to liquid level, because far from stirring vane 14 under the action of gravity, sink, thereby realize the continuous change of organism position. This design also helps to reduce the stirring dead angle of bottle bottom, prevent organism from sinking, help organism evenly distributed in reaction liquid, thereby make the reaction fully carry out.

[0029] Specifically see the following examples.

[0030] Example one:

[0031] See Figure 1 The bioreactor shown in a preferred embodiment of the application includes reaction bottle 1 and power assembly 2, reaction bottle 1 has a plurality of different volume specifications, and each specification of reaction bottle 1 is matched with power assembly 2, so that the bioreactor is suitable for reaction systems of different volumes. The whole reaction bottle 1 is made of medical plastic, which has low cost and is a disposable consumable product. It prevents biological contamination caused by difficult cleaning of the reaction bottle 1 and helps to improve the culture effect.

[0032] The power assembly 2 includes a housing 21, a mounting base 22, a stirring motor 23, an input component, and a controller. The housing 21 is generally constructed as a vertical cylinder. The bottom of the housing 21 expands to form a stabilizing portion 212, which improves the stability of the power assembly 2. In this embodiment, the stabilizing portion 212 is constructed as a cylinder coaxial with its upper portion. The top surface of the housing 21 protrudes upwards to form a positioning portion 211. The positioning portion 211 is constructed as a frustum with a diameter increasing from top to bottom and is coaxial with the stabilizing portion 212. A circular opening is formed at the top of the positioning portion 211, coaxial with it. The mounting base 22 is rotatably connected to the opening at the top of the positioning portion 211, and the mounting base 22 is entirely embedded inside the housing 21. The mounting base 22 is generally constructed as a hollow cylinder with an open top. The stirring motor 23 is installed inside the housing 21 and fixedly connected to the inner wall of the housing 21 and the outer wall of the mounting base 22, driving the mounting base 22 to rotate controllably along its central axis. The input components include several buttons and a display, and are mounted on the outer wall of the housing 21. The controller, installed inside the housing 21, is electrically connected to the stirring motor 23, the input components, and an external power supply, facilitating the controllable rotation of the mounting base 22.

[0033] In this embodiment, the power assembly 2 also includes a counterweight 24 installed at the bottom of the housing 21. The counterweight 24 is constructed in an annular shape to fit the stabilizing part 212 of the housing 21 and has a large mass, which helps to stabilize the center of gravity of the power assembly 2 and prevent the power assembly 2 from tipping over, thereby improving the safety of the bioreactor.

[0034] The reaction flask 1 includes a flask body 11, a cap 12 fitted to the flask body 11, a rotating shaft 13 rotatably connected to the bottom of the flask body 11, a stirring blade 14 connected to the rotating shaft 13, and a connector 15 for connecting the bottom of the flask body 11 and the rotating shaft 13.

[0035] The bottle body 11 includes a receiving portion 111 and multiple inlets / outlets 112. The receiving portion 111 is cylindrical in shape, with graduated markings on its outer wall and a closed bottom, used to hold the reaction solution. Two inlets / outlets 112 are connected to the top of the receiving portion 111, both cylindrical in shape and forming an angle with the receiving portion 111, and are symmetrically arranged facing opposite directions. In this embodiment, sampling is performed through two inlets / outlets 112, which helps reduce the risk of biocontamination. The inlet and outlet can also be used for adding different solutions, improving the versatility of the bottle body 11. Two caps 12 are fitted to the two inlets / outlets 112 respectively, and are constructed in different colors for easy differentiation. A fitting groove 113 is formed at the center of the bottom of the bottle body 11, which matches the positioning part 211 of the shell 21. The thickness of the bottom of the bottle body 11 is large, which is greater than the depth of the fitting groove 113, so that the inner wall of the bottom of the bottle body 11 is flat, reducing the formation of dead corners in the stirring.

[0036] The rotating shaft 13 is integrally configured as a cylinder, and penetrates the bottom of the bottle 11 at the center of the bottom and perpendicularly to the bottom of the bottle 11. The connecting piece 15 is connected between the bottom of the bottle 11 and the rotating shaft 13, and is configured as a rotating shaft sealing structure, so that the rotating shaft 13 is freely rotatable relative to the bottle 11, and the bottom of the bottle 11 is sealed. In the embodiment, the connecting piece 15 is configured as a rotating floating plug. The exposed part of the rotating shaft 13 outside the bottle 11 is an extended end 131 of the rotating shaft 13, and the extended end 131 of the rotating shaft 13 is fitted to the inner wall of the mounting seat 22, and the outer wall thereof is partially protruded to form a plurality of clamping portions 132. The clamping portions 132 are configured as rectangular long strips parallel to the rotating shaft 13, and the inner wall of the mounting seat 22 is formed with a plurality of clamping grooves 221 fitted to the clamping portions 132 of the rotating shaft 13, so that the extended end 131 of the rotating shaft 13 is detachably connected to the mounting seat 22. The extended end 131 of the rotating shaft 13 is integrally embedded in the fitting groove 113 of the bottle 11, so as to protect the extended end 131 of the rotating shaft 13, and facilitate the placement of the reaction bottle 1 on a plane, thereby facilitating the storage of the reaction bottle 1. In the embodiment, the bottom of each clamping portion 132 of the rotating shaft 13 and the bottom of the clamping groove 221 of the mounting seat 22 are connected with mutually fitted magnetic sheets, so as to facilitate the detachable connection of the reaction bottle 1 and the power assembly 2.

[0037] The stirring blades 14 are arranged in the bottle 11, and a plurality of stirring blades 14 are connected to the same height position on the outer wall of the rotating shaft 13. In the embodiment, the stirring blades 14 are arranged in an inclined manner, so as to facilitate the formation of upward liquid flow during stirring. In other embodiments, the stirring blades 14 can have other structures.

[0038] In the embodiment, the bottle 11 is made of polypropylene material, which is helpful to reduce the cost and is suitable for the reaction environment.

[0039] In the utility model, the stirring blades 14 are arranged adjacent to the bottom of the bottle 11, so as to improve the stirring effect. The detachable connection of the reaction bottle 1 and the power assembly 2 makes the bioreactor suitable for reaction systems with different volumes. By using the reaction bottle 1 as disposable consumables, the problem of high difficulty in cleaning the stirring blades 14 is avoided, which is helpful to reduce the risk of biological pollution.

[0040] Embodiment two:

[0041] Please refer to Figure 1 and Figure 2 The difference between the embodiment and the embodiment one is that the embodiment does not have the positioning portion 211 and the fitting groove 113, and the bottom surface of the bottle 11 and the top surface of the shell 21 are both configured as flat surfaces.

[0042] Any combination of the technical features in the above-described embodiments can be made, and for the sake of brevity, not all possible combinations are described, however, as long as the combination of the technical features does not exist in contradiction, it shall be considered as within the scope of the present disclosure.

[0043] The above-described embodiments only express several implementation manners of the present application, the description is relatively specific and detailed, but it shall not be understood as a limitation on the scope of the present application patent. It should be pointed out that, for ordinary skilled in the art, under the premise of not departing from the concept of the present application, a number of modifications and improvements can be made, which all belong to the protection scope of the present application. Therefore, the protection scope of the present application patent shall be subject to the appended claims.

Claims

1. A bioreactor, characterized in that, The utility model relates to a reaction bottle and power assembly, and belongs to the technical field of laboratory equipment. The reaction bottle comprises a bottle body (11), a cover body (12) matched with the bottle body (11), a rotating shaft (13) penetrating through the bottom of the bottle body (11) and rotationally connected with the bottle body (11), and a plurality of stirring blades (14) connected with the rotating shaft (13), wherein the extending end (131) of the rotating shaft (13) is located outside the bottle body (11). The power assembly (2) comprises a shell (21) and a stirring motor (23), the extending end (131) of the rotating shaft (13) is connected with the stirring motor (23) arranged inside the shell (21), and the stirring motor (23) is used to drive the rotating shaft (13) to rotationally move along the central axis in a controllable manner.

2. The bioreactor of claim 1, wherein, The power assembly (2) further comprises a mounting seat (22) arranged at the opening of the shell (21) and connected with the stirring motor (23) to rotationally move under the driving of the stirring motor (23), and the extending end (131) of the rotating shaft (13) is detachably connected with the mounting seat (22).

3. The bioreactor of claim 2, wherein, The central part of the top of the shell (21) is provided with a raised positioning part (211), and the bottom of the reaction bottle (1) is formed with a matching groove (113) matched with the positioning part (211), and the mounting seat (22) is arranged at the top of the positioning part (211).

4. The bioreactor of claim 3, wherein, The positioning part (211) is integrally formed in the shape of a circular truncated cone, and the diameter of the top of the positioning part (211) is smaller than the diameter of the bottom.

5. The bioreactor of claim 3, wherein, The thickness of the bottom of the reaction bottle (1) is greater than the depth of the matching groove (113).

6. The bioreactor of claim 2, wherein, The outer wall of the extending end (131) of the rotating shaft (13) is provided with a plurality of clamping parts (132) formed in a raised manner, and the clamping parts (132) are formed in the shape of a long strip parallel to the rotating shaft (13).

7. The bioreactor of claim 2, wherein, A plurality of reaction bottles (1) with different volumes are matched with the power assembly (2).

8. The bioreactor of claim 1, wherein, The bottle body (11) is provided with a plurality of inlets and outlets (112) for communicating the inside and outside of the bottle body (11), and a plurality of cover bodies (12) are respectively matched with the inlets and outlets (112).

9. The bioreactor of claim 1, wherein, The power assembly (2) further comprises a counterweight (24) arranged at the bottom of the power assembly (2) close to the edge to stabilize the center of gravity of the power assembly (2).

10. The bioreactor of claim 1, wherein, The reaction bottle (1) is made of medical plastic.

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