Umbilical cord blood NK cell separation auxiliary device

Abstract

The utility model relates to the technical field of cell separation, in particular to an umbilical cord blood NK cell separation auxiliary device which comprises a base, separation equipment and a sealing box, a cylinder is fixedly connected to the inner wall of the sealing box, sliding holes are formed in the two sides of the cylinder, the inner walls of the two sliding holes are slidably connected with the same push rod, and one end of the push rod is fixedly connected with a push plate. The surface of the push rod is fixedly connected with a rubber piston plate, the inner wall of the cylinder is attached to the surface of the rubber piston plate, the rubber piston plate divides the cylinder into a first cavity and a second cavity, one end of the first cavity and one end of the second cavity are fixedly communicated with liquid inlet pipes and liquid outlet pipes, and the surfaces of the two liquid inlet pipes and the surfaces of the two liquid outlet pipes are fixedly provided with one-way valves. Nozzles are fixedly connected to one ends of the two liquid outlet pipes, and a rotating unit is arranged on the surface of the push rod; the problems that after disinfection is conducted through an existing disinfectant, the catheter cannot be cleaned, the disinfectant is left at a catheter opening, cell death or activity reduction is easily caused, and follow-up NK cell separation is affected are solved.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell separation technical field, especially a kind of umbilical cord blood NK cell separation auxiliary device. BACKGROUND

[0002] Umbilical cord blood NK cell has strong antitumor activity, can inhibit the growth and spread of tumor by recognizing and killing cancer cells, in cancer treatment, after separating and expanding umbilical cord blood NK cell, it can be used to treat a variety of cancers, therefore, umbilical cord blood NK cell separation auxiliary device will be used.

[0003] In prior art, such as the Chinese patent with announcement number CN214612409U, a kind of umbilical cord blood stem cell separation device is disclosed, by being equipped with entering catheter in one end of separation tank, umbilical cord blood can be made into the inside of separation tank by entering catheter, one end of separation tank is fixedly connected with placing box, entering catheter can be protected, liquid storage box can place disinfectant, press rod in the direction of fixed rod away, so that one end of flow groove equipped with in the inside of moving block is opposite first connecting port, the other end of flow groove is opposite second connecting port, so that disinfectant in the inside of flow pipe will flow out from the inside of flow groove, it is convenient to disinfect the entrance of entering catheter before using separation machine.

[0004] In the above technical scheme, before separating umbilical cord blood cell, disinfectant is used to disinfect the entrance of entering catheter, however, after disinfection, the entrance of entering catheter is not cleaned, which can cause disinfectant to remain in the entrance of catheter, because the activity and purity of NK cell are key factors for evaluating its therapeutic effect, chemical components in disinfectant can damage the structure and function of cell, leading to cell death or activity reduction, thereby affecting subsequent separation of NK cell.

[0005] Therefore, an umbilical cord blood NK cell separation auxiliary device is proposed. UTILITY MODEL CONTENT

[0006] The utility model aims at providing an umbilical cord blood NK cell separation auxiliary device to solve the problems raised in the background.

[0007] The utility model discloses a technical scheme is: a kind of umbilical cord blood NK cell separation auxiliary device, including base, the upper surface of base is equipped with through-hole, the inner wall of through-hole is provided with separation equipment, the upper surface of separation equipment is fixedly connected with sealed box, the inner wall of sealed box is fixedly connected with cylinder, the both sides of cylinder are equipped with slide hole, the inner wall of two slide holes is slidably connected with same push rod, one end of push rod is fixedly connected with push plate, the both sides of cylinder are fixedly connected with sealing ring, the surface of push rod and the inner wall of two sealing rings are adaptively slidably connected, the surface of push rod is fixedly connected with rubber piston plate, the inner wall of cylinder and the surface of rubber piston plate are attached, the rubber piston plate divides cylinder into cavity one and cavity two, one end of cavity one and cavity two is fixedly connected with liquid inlet pipe and liquid outlet pipe, the surface of two liquid inlet pipes and two liquid outlet pipes is fixedly installed with one-way valve, one end of two liquid outlet pipes is fixedly connected with spray head, the surface of push rod is provided with rotation unit.

[0008] In certain embodiments, the rotation unit includes a connecting rod fixedly connected to the surface of the push rod, one end of the connecting rod is fixedly connected with a rack, the upper surface of the separation equipment is fixedly connected with a rotating seat, the inner wall of the rotating seat is rotatably connected with a guide pipe, the surface of the guide pipe is fixedly connected with a gear, and the gear is engaged with the rack.

[0009] In certain embodiments, the inner wall of the sealed box is fixedly connected with a liquid storage box, the inner wall of the liquid storage box is fixedly connected with a partition, and the top end of two liquid outlet pipes is fixedly communicated with the lower surface of the liquid storage box on both sides of the partition.

[0010] In certain embodiments, the surface of the sealed box is provided with a placing mechanism, and the upper surface of the sealed box is provided with two filling openings.

[0011] In certain embodiments, the upper surface of the base is provided with a storage cylinder, the upper surface of the storage cylinder is fixedly communicated with a guide pipe, and one end of the guide pipe is fixedly communicated with the upper surface of the separation equipment.

[0012] Compared with the prior art, the utility model has the following advantages:

[0013] The utility model discloses a push rod is pushed, and the push rod drives rubber piston plate extrusion cavity no. 2, can make the disinfectant inside cavity no. 2 through liquid outlet pipe and spray head and spray the surface of import pipe and disinfect, simultaneously when pushing the push rod, the push rod drives the rack movement and drives the gear rotation, can realize that import pipe rotates on the rotating seat, thereby improves the disinfection range, when rubber piston plate extrusion cavity no. 2, can improve the inside space of cavity no. 1, thereby through liquid inlet pipe and take physiological saline in cavity no. 1 in the storage box, after disinfection, pull the push rod, and rubber piston plate extrusion cavity no. 1 can be washed to the surface of import pipe through liquid outlet pipe and spray head, and the residual disinfectant can be washed.

[0014] Solve the current use disinfectant disinfection, can not be washed, lead to disinfectant residue in catheter port to cause cell death or activity reduction, thereby affecting the subsequent separation NK cell problem. BRIEF DESCRIPTION OF DRAWINGS

[0015] The utility model makes further explanation in combination with the drawings and examples:

[0016] Figure 1 It is the three-dimensional structure schematic diagram provided in an embodiment of the utility model;

[0017] Figure 2 It is the disinfection auxiliary assembly structure schematic diagram provided in an embodiment of the utility model;

[0018] Figure 3 It is the disinfection auxiliary assembly cut structure schematic diagram provided in an embodiment of the utility model;

[0019] Figure 4 It is the disinfection auxiliary assembly provided in an embodiment of the utility model Figure 3 Amplification structure schematic diagram in place A.

[0020] BRIEF DESCRIPTION OF DRAWINGS

[0021] 1, base;2, separation equipment;3, storage cylinder;4, lead-out pipe;5, sealed box;6, rotating seat;7, import pipe;8, placing mechanism;9, storage box;10, partition;11, cylinder;12, cavity no. 1;13, push rod;14, push plate;15, rack;16, gear;17, liquid outlet pipe;18, spray head;19, connecting rod;20, sealing ring;21, rubber piston plate;22, liquid inlet pipe;23, check valve;24, cavity no. 2. DETAILED DESCRIPTION

[0022] The utility model discloses below make detailed instructions, clear, complete to the technical scheme of the embodiment of the utility model, obviously, the described embodiment is only a part of the embodiment of the utility model, not all the embodiment. Based on the embodiment in the utility model, all other embodiments that the ordinary skill in the art obtains without making creative labor belong to the range of protection of the utility model.

[0023] The utility model discloses an umbilical cord blood NK cell separation auxiliary device, and the technical scheme of the utility model is:

[0024] As shown in figure, Figures 1-4 A kind of umbilical cord blood NK cell separation auxiliary device, including base 1, the upper surface of base 1 is provided with through hole, the inner wall of through hole is provided with separation equipment 2, the upper surface of separation equipment 2 is fixedly connected with sealed box 5, the inner wall of sealed box 5 is fixedly connected with cylinder 11, both sides of cylinder 11 are provided with sliding hole, the inner wall of two sliding holes is slidably connected with same push rod 13, one end of push rod 13 is fixedly connected with push plate 14, both sides of cylinder 11 are fixedly connected with sealing ring 20, the surface of push rod 13 and the inner wall of two sealing rings 20 are adaptively slidably connected, push plate 14 can be conveniently handheld, the surface of push rod 13 is fixedly connected with rubber piston plate 21, rubber piston plate 21 adopts flexible structure, can realize and the inner wall of cylinder 11 closely adhere, can improve air tightness, the inner wall of cylinder 11 and the surface of rubber piston plate 21 are pasted, rubber piston plate 21 divides cylinder 11 into cavity one 12 and cavity two 24, physiological saline and disinfectant are drawn into cavity one 12 and cavity two 24 respectively, one end of cavity one 12 and cavity two 24 is fixedly connected with liquid inlet pipe 22 and liquid outlet pipe 17, the surface of two liquid inlet pipes 22 and two liquid outlet pipes 17 is fixedly installed with one-way valve 23, one end of two liquid outlet pipes 17 is fixedly connected with spray head 18, the surface of push rod 13 is provided with rotating unit.

[0025] It should be noted that: the separation equipment 2 in the application and the separation equipment 2 in the prior art have the same structure principle, the one-way valve 23 is prior art, and the one-way valve 23 is an important element for controlling the direction of fluid in a fluid system. It allows fluid to flow freely in one direction, but blocks flow in the opposite direction.

[0026] As shown in figure, Figure 3 In an embodiment, the rotating unit includes a connecting rod 19 fixedly connected to the surface of the push rod 13, one end of the connecting rod 19 is fixedly connected with a rack 15, the upper surface of the separation equipment 2 is fixedly connected with a rotating seat 6, the inner wall of the rotating seat 6 is rotatably connected with an inlet tube 7, the surface of the inlet tube 7 is fixedly connected with a gear 16, the gear 16 is engaged with the rack 15, the rotation of the inlet tube 7 can be realized by the gear 16 and the rack 15, thereby increasing the area of the disinfectant sprayed on the surface of the inlet tube 7.

[0027] As Figure 1 and Figure 2 shown, in an embodiment, the inner wall of the sealing box 5 is fixedly connected with the liquid storage box 9, the inner wall of the liquid storage box 9 is fixedly connected with the partition plate 10, the top ends of the two liquid outlet pipes 17 are fixedly communicated with the lower surfaces of the liquid storage boxes 9 on the two sides of the partition plate 10, and the partition plate 10 in the liquid storage box 9 divides the inside of the liquid storage box 9 into two spaces with different volumes, the space for physiological saline is larger than the space for disinfectant, so that the flushing can be better achieved.

[0028] The surface of the sealing box 5 is provided with the placing mechanism 8, the upper surface of the sealing box 5 is provided with two filling openings, and the placing mechanism 8 is also the same as the placing mechanism 8 in the prior art in structure and principle, so that the umbilical cord blood can be introduced.

[0029] The upper surface of the base 1 is provided with the storage cylinder 3, the upper surface of the storage cylinder 3 is fixedly communicated with the guide-out pipe 4, and one end of the guide-out pipe 4 is fixedly communicated with the upper surface of the separation device 2.

[0030] Specific working method is: when using, the separation device 2 is provided with the guide-in pipe 7, the umbilical cord blood can be better introduced into the inside of the separation device 2 through the guide-in pipe 7, so that the separation device 2 can better separate NK cells, the separation device 2 is installed with the sealing box 5, so that the guide-in pipe 7 can be better protected, the disinfectant and physiological saline can be better placed in the liquid storage box 9 through the filling openings in the upper portion of the sealing box 5, before introducing the umbilical cord blood, the push rod 13 is pushed, the rubber piston plate 21 is driven to extrude the cavity two 24 by the push rod 13, so that the disinfectant in the cavity two 24 can be sprayed to the surface of the guide-in pipe 7 through the liquid outlet pipe 17 and the spray head 18 for disinfection, at the same time, when the push rod 13 is pushed, the rack 15 is driven to move and the gear 16 is driven to rotate, so that the guide-in pipe 7 can be rotated on the rotating seat 6, thereby improving the disinfection range, at the same time, when the rubber piston plate 21 extrudes the cavity two 24, the inside space of the cavity one 12 can be improved, so that the physiological saline in the inside of the liquid storage box 9 can be sucked into the cavity one 12 through the liquid inlet pipe 22, after disinfection, the push rod 13 is pulled, the rubber piston plate 21 extrudes the cavity one 12, so that the surface of the guide-in pipe 7 can be flushed through the liquid outlet pipe 17 and the spray head 18, the residual disinfectant can be flushed, the residual disinfectant in the guide pipe is easy to cause cell death or activity reduction, thereby affecting the subsequent separation of NK cells.

[0031] The technical means disclosed in the utility model scheme are not limited to the technical means disclosed in the above technical means, and also include technical schemes composed of equivalent replacement of the above technical features. The unfinished matters of the utility model belong to the common knowledge of those skilled in the art.

Claims

1. An umbilical cord blood NK cell separation aid device comprising a base (1), characterized in that: The upper surface of the base (1) is provided with a through hole, the inner wall of the through hole is provided with a separation device (2), the upper surface of the separation device (2) is fixedly connected with a sealing box (5), the inner wall of the sealing box (5) is fixedly connected with a cylinder (11), both sides of the cylinder (11) are provided with sliding holes, the inner walls of the two sliding holes are slidably connected with the same push rod (13), one end of the push rod (13) is fixedly connected with a push plate (14), both sides of the cylinder (11) are fixedly connected with sealing rings (20), the surface of the push rod (13) is slidably connected with the inner walls of the two sealing rings (20), the surface of the push rod (13) is fixedly connected with a rubber piston plate (21), the inner wall of the cylinder (11) is attached to the surface of the rubber piston plate (21), the rubber piston plate (21) divides the cylinder (11) into cavity one (12) and cavity two (24), one end of the cavity one (12) and the cavity two (24) is fixedly connected with a liquid inlet pipe (22) and a liquid outlet pipe (17), the surfaces of the two liquid inlet pipes (22) and the two liquid outlet pipes (17) are fixedly installed with one-way valves (23), one end of the two liquid outlet pipes (17) is fixedly connected with a spray head (18), the surface of the push rod (13) is provided with a rotating unit.

2. The umbilical cord blood NK cell separation aid of claim 1, wherein: The rotating unit comprises a connecting rod (19) fixedly connected to the surface of the push rod (13), one end of the connecting rod (19) is fixedly connected with a rack (15), the upper surface of the separation device (2) is fixedly connected with a rotating seat (6), the inner wall of the rotating seat (6) is rotatably connected with a guide pipe (7), the surface of the guide pipe (7) is fixedly connected with a gear (16), the gear (16) is engaged with the rack (15).

3. The umbilical cord blood NK cell separation aid of claim 1, wherein: The inner wall of the sealing box (5) is fixedly connected with a liquid storage box (9), the inner wall of the liquid storage box (9) is fixedly connected with a partition plate (10), the top ends of the two liquid outlet pipes (17) are fixedly communicated with the lower surfaces of the liquid storage boxes (9) on both sides of the partition plate (10).

4. The umbilical cord blood NK cell isolation aid of claim 3, wherein: The surface of the sealing box (5) is provided with a placing mechanism (8), the upper surface of the sealing box (5) is provided with two filling openings.

5. The umbilical cord blood NK cell isolation aid of claim 1, wherein: The upper surface of the base (1) is provided with a storage cylinder (3), the upper surface of the storage cylinder (3) is fixedly communicated with a guide pipe (4), one end of the guide pipe (4) is fixedly communicated with the upper surface of the separation device (2).

Images