Multi-cell co-culture device

Abstract

The utility model provides a multi-cell co-culture device which comprises a culture dish main body, the top of the culture dish main body is sleeved with a supporting cover body, and the top of the supporting cover body is sleeved with a top cover; a supporting plate is arranged in the supporting cover body, mounting holes and air holes are uniformly formed in the supporting plate, small culture chambers are arranged in the mounting holes, the upper ends of the small culture chambers are in magnetic attraction connection with the supporting plate, and micropores are uniformly formed in the bottoms and the outer sides of the small culture chambers; according to the culture device, the top ends of the small culture chambers are magnetically attracted to the supporting plate, the small culture chambers can be stably fixed, pollution caused by mutual collision between the small culture chambers during movement is avoided, and the culture effect is guaranteed.

Description

TECHNICAL FIELD

[0001] The utility model belongs to cell culture technical field, concretely relates to a kind of multi-cell co-culture device. BACKGROUND

[0002] Common cell culture device is mainly single cell line gas-liquid phase plane culture, its singleness and fixity limit the multi-directional development of cell culture, cannot satisfy the in-depth study of complex system such as the interaction between multiple cells.

[0003] In prior art, the Chinese utility model patent document with the authorization announcement No.CN203048950U discloses a kind of multi-cell co-culture device, by being placed in multiple cell culture chambers in culture dish can realize the co-culture of dozens of cells;But the stability of cell culture chamber in culture dish is insufficient, when moving culture dish, cell culture chamber is easy to slide in culture dish and collide with each other, cause mutual pollution between cell culture chamber, affect culture effect.

[0004] Therefore, it is needed to design a kind of multi-cell co-culture device which can stably fix culture chamber, avoid mutual pollution and ensure culture effect to solve the technical problems faced at present. SUMMARY

[0005] In view of the deficiencies in the prior art, the utility model provides a kind of multi-cell co-culture device which can stably fix culture chamber, avoid mutual pollution and ensure culture effect.

[0006] The technical scheme of the utility model is as follows: a multi-cell co-culture device, comprising a culture dish main body, a support cover is sleeved on the top of the culture dish main body, and a top cover is sleeved on the top of the support cover;The inside of the support cover has a support plate, the support plate is uniformly provided with mounting holes and air holes, the inside of the mounting hole is provided with a culture chamber, the upper end of the culture chamber is magnetically connected with the support plate, and the bottom and the outside of the culture chamber are uniformly provided with micropores.

[0007] The bottom outside of the support plate is fixedly provided with a connecting edge, the top of the connecting edge is fixedly provided with a supporting edge, the connecting edge is screw-connected with the top end outside of the culture dish main body, and the top cover is movably sleeved on the top end outside of the supporting edge.

[0008] The top end outside of the culture dish main body is provided with external thread, and the inside of the connecting edge is provided with internal thread matched with the external thread.

[0009] The mounting hole is a center through hole opened in the support plate, the culture chamber is a barrel-shaped structure matched with the mounting hole, the top end outside of the culture chamber has a blocking edge, and the blocking edge is magnetically connected with the support plate.

[0010] The bottom of the baffle is fixedly provided with a magnetic attraction piece, and the support plate is fixedly provided with a magnetic attraction ring concentric with the mounting hole.

[0011] The top of the baffle is provided with a hand buckle part on both sides, the hand buckle part is an integral structure with the baffle, and the hand buckle part is provided with a hand buckle groove on the side away from the baffle center.

[0012] The utility model discloses the beneficial effects of the following:

[0013] (1) in the utility model, the inside of multiple culture chambers on the support plate can be inoculated with different kinds of cells respectively, then the supporting cover body is installed on the top of the culture dish body containing the culture medium, the bottom end of the culture chamber is immersed in the inside of the culture medium, and the main components in the culture medium can enter the culture chamber through the micropore to provide supply for cell culture, and the cells in the culture chamber cannot pass through the micropore;

[0014] (2) the top end of the culture chamber is magnetically attracted on the support plate, so that the culture chamber can be stably fixed, the mutual collision between the culture chambers during movement is avoided, and the culture effect is guaranteed;

[0015] (3) the top cover can be opened, and the culture condition of the cells in the culture chamber can be observed conveniently. DRAWINGS

[0016] Figure 1 It is one of the structure schematic views of the multi-cell co-culture device in the utility model.

[0017] Figure 2 It is the second structure schematic view of the multi-cell co-culture device in the utility model.

[0018] Figure 3 It is the structure schematic view of the culture chamber in the utility model. Figure 2 It is the sectional view of A-A in the utility model.

[0019] Figure 4 It is the structure schematic view of the culture chamber in the utility model. CONCRETE IMPLEMENTATION

[0020] Various exemplary embodiments of the present application will now be described in detail with reference to the accompanying drawings. The description of the exemplary embodiments is merely illustrative in nature and is in no way intended to limit the application, its application or uses. The application can be implemented in any of numerous ways, not just the examples described herein. The examples are provided so that this application will be thorough and complete, and will fully convey the scope of the application to those skilled in the art. It should be noted that the relative arrangement of components and steps, the numerical expressions, and the numerical values set forth in these examples are presented only as examples and are not intended to be limiting unless otherwise specifically stated.

[0021] The terms "first", "second", and similar terms used in the present application do not denote any order, number or importance, but are only used to distinguish different parts. The terms "comprise", "comprising", and similar terms mean that the elements before the term encompass the elements listed after the term, and do not exclude the possibility of also encompassing other elements. The terms "up", "down", "left", "right", and the like are only used to indicate relative positional relationships, and when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0022] As shown in Figures 1 to 3 The multi-cell co-culture device includes a culture dish body 1, a support cover 2 is sleeved on the top of the culture dish body 1, and a top cover 3 is sleeved on the top of the support cover 2. The inside of the support cover 2 has a support plate 21, and the support plate 21 is uniformly provided with mounting holes 24 and air holes. The inside of the mounting hole 24 is provided with a culture chamber 4, the upper end of the culture chamber 4 is magnetically connected with the support plate 21, and the bottom and the outside of the culture chamber 4 are uniformly provided with micropores. In this embodiment, the inside of the plurality of culture chambers 4 on the support plate 21 can be inoculated with different types of cells, respectively. Then the support cover 2 is installed on the top of the culture dish body 1 containing the culture medium, the bottom end of the culture chamber 4 is immersed in the inside of the culture medium, and the main components in the culture medium can enter the culture chamber 4 through the micropores to provide supply for cell culture, while the cells in the culture chamber 4 will not pass through the micropores. The top end of the culture chamber 4 is magnetically attracted to the support plate 21, which can stably fix the culture chamber 4 and avoid mutual collision between the culture chambers 4 during movement to cause pollution, thereby ensuring the culture effect. The top cover 3 can be opened to facilitate observation of the culture condition of the cells in the culture chamber 4.

[0023] In some embodiments, the bottom outer side of the support plate 21 is fixedly provided with a connecting edge 22, the top of the connecting edge 22 is fixedly provided with a supporting edge 23, the connecting edge 22 is threadedly connected with the top outer side of the culture dish body 1, the top cover 3 is movably sleeved on the top outer side of the supporting edge 23, and the support cover 2 is threadedly connected with the top outer side of the culture dish body 1 through the connecting edge 22. The connection is high in stability and can provide relatively stable support for the culture chamber 4. The top cover 3 is movably sleeved on the top outer side of the supporting edge 23, so that the top cover 3 can be easily opened to observe the state of cells in the culture chamber 4.

[0024] In some embodiments, the top outer side of the culture dish body 1 is provided with external threads, and the inner side of the connecting edge 22 is provided with internal threads matched with the external threads.

[0025] In some embodiments, as shown in Figure 4 the mounting hole 24 is a circular central through hole formed in the support plate 21, the culture chamber 4 is a barrel-shaped structure matched with the mounting hole 24, the top outer side of the culture chamber 4 is provided with a blocking edge 41, the blocking edge 41 is an integral structure with the culture chamber 4, and the blocking edge 41 is magnetically connected with the support plate 21.

[0026] In some embodiments, the bottom of the blocking edge 41 is fixedly provided with a magnetic attraction piece 42, and the support plate 21 is fixedly provided with a magnetic attraction ring concentric with the mounting hole 24, and the magnetic attraction piece 42 is magnetically connected with the magnetic attraction ring. Specifically, the magnetic attraction ring is a magnet ring, and the magnetic attraction piece 42 is an iron piece.

[0027] In some embodiments, the top of the blocking edge 41 is provided with a hand buckle portion 43 on both sides, the hand buckle portion 43 is an integral structure with the blocking edge 41, the hand buckle portion 43 is provided with a hand buckle groove 44 starting from the side away from the center of the blocking edge, and the culture chamber 4 can be conveniently taken out of the support cover 2 through the hand buckle groove 44 formed in the hand buckle portion 43.

[0028] Thus far, the embodiments of the present application have been described in detail. In order to avoid obscuring the concept of the present application, some details known in the art are not described. Those skilled in the art can fully understand how to implement the technical solutions disclosed herein according to the above description.

[0029] The above-described embodiments only express some implementation manners of the present application, and the description is relatively specific and detailed, but it should not be understood as a limitation on the scope of the utility model patent. It should be pointed out that, for those skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which all belong to the protection scope of the present application. Therefore, the protection scope of the utility model patent should be subject to the appended claims.

Claims

1. A multi-cell co-culture device, characterized by: The application relates to a culture dish, which comprises a culture dish body, a supporting cover body sleeved on the top of the culture dish body, and a top cover sleeved on the top of the supporting cover body; the inside of the supporting cover body is provided with a supporting plate, the supporting plate is uniformly provided with mounting holes and air holes, the inside of the mounting holes is provided with culture cells, the upper end of the culture cell is magnetically connected with the supporting plate, and the bottom and the outside of the culture cell are uniformly provided with micropores.

2. The multi-cell co-culture device of claim 1, wherein: The bottom outside of the supporting plate is fixedly provided with a connecting edge, the top of the connecting edge is fixedly provided with a supporting edge, the connecting edge is screw-connected with the top outside of the culture dish body, and the top cover is movably sleeved on the top outside of the supporting edge.

3. The multi-cell co-culture device of claim 2, wherein: The top outside of the culture dish body is provided with external threads, and the inside of the connecting edge is provided with internal threads matched with the external threads.

4. The multi-cell co-culture device of claim 1, wherein: The mounting holes are concentric through holes provided on the supporting plate, the culture cells are barrel-shaped structures matched with the mounting holes, the top outside of the culture cell is provided with a blocking edge, and the blocking edge is magnetically connected with the supporting plate.

5. The multi-cell co-culture device of claim 4, wherein: The bottom of the blocking edge is fixedly provided with a magnetic attraction piece, the supporting plate is fixedly provided with a magnetic attraction ring concentric with the mounting holes, and the magnetic attraction piece is magnetically connected with the magnetic attraction ring.

6. The multi-cell co-culture device of claim 4, wherein: The top of the blocking edge is provided with hand buckle parts on both sides, the hand buckle parts are integrated with the blocking edge, and the hand buckle parts are provided with hand buckle grooves on the sides away from the center of the blocking edge.

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