Single-molecule immunofluorescence analyzer

Abstract

The utility model belongs to an immune technology, and particularly relates to a single-molecule immunofluorescence analyzer which comprises a light source and a convergent lens, an optical channel is formed in the guide piece; the moving part is arranged on the outer side of the guide part and moves along the guide part under driving; the fixed part is used for fixing the convergent lens on the movable part, and light penetrating through the convergent lens penetrates through the optical channel; the transmission mechanism is used for converting rotation of the rotating mechanism into rotation of the moving part. The utility model has the advantages of high adjustment precision and the like, and is used in optical focusing.

Description

TECHNICAL FIELD

[0001] The utility model relates to immunological technique, especially single molecule immunofluorescence analyzer. BACKGROUND

[0002] In the light detection module of the full-automatic single molecule immunofluorescence analyzer, laser spot needs to be focused on the microfluidic chip detection flow channel of 15 mu m, and the fluorescence magnetic microparticle to be detected is excited. The focal length of the selected focusing lens is 3.4 mm, and the laser spot is 10 mu m. The spot is very small, and due to the manufacturing and installation process of parts, errors are caused. It is easy to cause the laser focusing point not to be on the chip flow channel, resulting in no signal value or weak signal value, thereby causing inaccurate sample detection of the instrument and misjudgment of the disease of the patient. Therefore, the focal length direction of the lens needs to be adjustable, and the adjustment accuracy is very high. The design value is 5 mu m step, and the external instrument can be debugged.

[0003] The solution in the prior art is that the lens or lens adjusting device is mostly fixed in a part to adjust the lens left and right and front and back directions. The lens focal length is mostly adjusted by coarse adjustment, and the precision is 0.1 mm level. UTILITY MODEL CONTENT

[0004] To solve the above problems in the prior art, the utility model provides a single molecule immunofluorescence analyzer.

[0005] The utility model aims to realize the following technical scheme:

[0006] A single molecule immunofluorescence analyzer, comprising a light source and a converging lens, wherein the single molecule immunofluorescence analyzer further comprises:

[0007] A guide piece, which has an optical channel inside;

[0008] A moving piece, which is arranged outside the guide piece and moves along the guide piece under the drive;

[0009] A fixing piece, which is used for fixing the converging lens on the moving piece, and the light passing through the converging lens passes through the optical channel;

[0010] A rotating mechanism and a transmission mechanism, which are used for converting the rotation of the rotating mechanism into the rotation of the moving piece.

[0011] Compared with the prior art, the utility model has the beneficial effects that:

[0012] 1. High adjustment accuracy;

[0013] The matching of the first set of teeth and the second set of teeth and the matching between the internal thread and the external thread make the adjustment step of the converging lens reach the micron level;

[0014] 2. Good adjustment stability;

[0015] The spring pushes against the moving part, eliminates the thread gap, and improves the adjustment stability of the converging lens;

[0016] 3. Simple structure;

[0017] The guide, the rotating shaft and the hand nut are conventional components, and the gear transmission and the thread cooperation are common transmission modes in the field, so that the structure is simple and the operation is convenient. BRIEF DESCRIPTION OF DRAWINGS

[0018] The disclosure of the present application will become more apparent from the following description with reference to the attached drawings. It is easily understood by those skilled in the art that the drawings are only used to illustrate the technical solutions of the present application, and are not intended to limit the protection scope of the present application. In the drawings:

[0019] Fig. 1 is a structural schematic diagram of the optical part of the single molecule immunofluorescence analyzer of the present application;

[0020] Fig. 2 is a sectional structural schematic diagram of the optical part of the present application. DETAILED DESCRIPTION

[0021] Figs. 1-2 The following description describes optional specific embodiments of the present application to teach those skilled in the art how to implement and reproduce the present application. In order to teach the technical solutions of the present application, some conventional aspects have been simplified or omitted. Those skilled in the art should understand that variations or substitutions derived from these specific embodiments will be within the scope of the present application. Those skilled in the art should understand that the following features can be combined in various ways to form multiple variations of the present application. Therefore, the present application is not limited to the following optional specific embodiments, but is only limited by the claims and their equivalents.

[0022] Example 1.

[0023] A single molecule immunofluorescence analyzer comprises an optical part, as shown in Figs. 1-2 The optical part comprises:

[0024] a light source and a converging lens 5;

[0025] The guide 1 has an optical channel 12 inside;

[0026] The moving part 4 is arranged outside the guide 1 and moves along the guide 1 under the drive;

[0027] The fixing member 6 is used for fixing the converging lens 5 on the moving member 4, and the light passing through the converging lens 5 passes through the optical channel;

[0028] The rotating mechanism and a transmission mechanism are used for converting the rotation of the rotating mechanism into the rotation of the moving member.

[0029] In order to make the moving member 4 move stably along the central axis direction of the guide member 1, further, the analyzer further comprises:

[0030] The spring 2 is arranged on the outer side of the guide member 1 and the lower side of the moving member 4, and hinders the downward movement of the moving member 4.

[0031] In order to drive the moving member 4 to rotate while having up and down movements, further, the rotating structure comprises a rotating shaft 8 and a bearing member 13, and the rotating shaft 8 is arranged on the bearing member 13.

[0032] The transmission mechanism comprises a first rotating member 9, and the first rotating member 9 is fixed on the rotating shaft 8 and connected with the moving member 4.

[0033] In order to make the moving member 4 move precisely along the central axis direction of the guide member 1, further, the outer edge of the first rotating member 9 has a first set of teeth, the outer side of the moving member 4 has a second set of teeth which is engaged with the first set of teeth, and the outer wall of the guide member 1 has an outer thread, and the inner wall of the moving member 4 has an inner thread which is matched with and connected with the outer thread.

[0034] In order to make the moving member 4 move precisely along the central axis direction of the guide member 1, further, the outer edge of the first rotating member 9 has a first set of teeth, the second rotating member 3 is fixed on the moving member 4 and has a second set of teeth which is engaged with the first set of teeth, and the outer wall of the guide member 1 has an outer thread, and the inner wall of the moving member 4 has an inner thread which is matched with and connected with the outer thread.

[0035] In order to make the rotating shaft 8 rotatable, further, the rotating shaft 8 is fixed on the bearing member 13 through a bearing 10, and the bearing member 13 is fixedly connected with the guide member 1.

[0036] Embodiment 2.

[0037] According to the application example of the single-molecule immunofluorescence analyzer in the embodiment 1 of the utility model.

[0038] In the application example, as Figs. 1-2As shown, in the optical part of the analyzer, the rotating shaft 8 is rotatably fixed on the bearing 13 through the bearing 10 and the circlip 11, and the cylindrical guide 1 with the optical channel 12 in the inside is fixed on the bearing 13. The end of the rotating shaft 8 is provided with the hand nut 7, and the first rotating part 9 in the form of the first gear is fixed on the rotating shaft 8. The central axes of the rotating shaft 8 and the first gear are collinear.

[0039] The inner diameter of the moving part 4 matches the outer diameter of the guide 1, and the inner wall of the moving part 4 is provided with the internal thread (fine thread) matched with the external thread (fine thread) of the outer wall of the guide 1, so that when the moving part 4 rotates around the guide 1, it moves up and down along the guide 1. The second rotating part 3 in the form of the second gear is fixed on the outer side of the moving part 4. The second gear and the first gear are engaged. The central axes of the guide 1, the moving part 4 and the first gear are collinear and parallel to the central axis of the rotating shaft 8.

[0040] The fixed part 6 fixes the converging lens 5 on the end of the moving part 4. The spring 2 is located on the outer side of the guide 1 and between the bearing 13 and the moving part 4, and is used to hinder the moving part 4 from moving downward, so as to eliminate the thread gap.

[0041] The working mode of the optical part of the analyzer in the embodiment is as follows:

[0042] When the converging lens 5 needs to move upward, the operator rotates the rotating shaft 8 in the positive direction through the hand nut 7, the first gear drives the second gear to rotate, the moving part 4 rotates around the central axis of the guide, and at the same time, moves upward along the central axis, the spring 2 pushes the moving part 4, the deformation amount becomes smaller, and the converging lens 5 moves upward along the central axis of the guide 1.

[0043] When the converging lens 5 needs to move downward, the operator rotates the rotating shaft 8 in the reverse direction through the hand nut 7, the first gear drives the second gear to rotate, the moving part 4 rotates around the central axis of the guide, and at the same time, moves downward along the central axis, the spring 2 pushes the moving part 4 and is compressed, the deformation amount becomes larger, and the converging lens 5 moves downward along the central axis of the guide 1.

[0044] The moving precision of the moving part 4 is adjusted by adjusting the diameters of the first gear and the second gear and the gap between the external thread and the internal thread, so that the moving distance is micron level.

[0045] Embodiment 3.

[0046] According to the application example of the single-molecule immunofluorescence analyzer in the embodiment 1 of the utility model, different from the embodiment 2, the second gear is not needed.

[0047] The second gear is not needed. The outer wall of the moving part is provided with the second set of teeth matched with the first set of teeth.

Claims

1. A single molecule immunofluorescence analyzer comprising a light source and a converging lens; characterized in that, The single-molecule immunofluorescence analyzer further comprises: a guide piece with an optical channel inside; a moving piece arranged outside the guide piece and moving along the guide piece under driving; a fixing piece for fixing the converging lens on the moving piece, light passing through the converging lens passing through the optical channel; a rotating mechanism and a transmission mechanism for converting rotation of the rotating mechanism into rotation of the moving piece.

2. The single molecule immunofluorescence analyzer according to claim 1, characterized in that, The analyzer further comprises: a spring arranged outside the guide piece and under the moving piece, hindering downward movement of the moving piece.

3. The single molecule immunofluorescence analyzer according to claim 1, wherein, The rotating mechanism comprises a rotating shaft and a bearing piece, the rotating shaft being arranged on the bearing piece; The transmission mechanism comprises a first rotating piece fixed on the rotating shaft and connected with the moving piece.

4. The single-molecule immunofluorescence analyzer according to claim 3, characterized in that, An outer edge of the first rotating piece has a first set of teeth, an outer side of the moving piece has a second set of teeth meshing with the first set of teeth; an outer wall of the guide piece has an outer thread, an inner wall of the moving piece has an inner thread matching and connected with the outer thread.

5. The single molecule immunofluorescence analyzer according to claim 3, wherein, An outer edge of the first rotating piece has a first set of teeth, a second rotating piece fixed on the moving piece has an outer side with a second set of teeth meshing with the first set of teeth; an outer wall of the guide piece has an outer thread, an inner wall of the moving piece has an inner thread matching and connected with the outer thread.

6. The single molecule immunofluorescence analyzer according to claim 3, wherein, An end of the rotating shaft is fixed with a hand screw nut.

7. The single molecule immunofluorescence analyzer according to claim 3, wherein, A central axis of the moving piece and a central axis of the rotating shaft are parallel.

8. The single molecule immunofluorescence analyzer according to claim 3, wherein, The rotating shaft is fixed on the bearing piece through a bearing, and the bearing piece is fixedly connected with the guide piece.

Images