Quantitative strain dripping ball device

Abstract

The utility model provides a quantitative strain ball dropping device, which relates to the technical field of microbial culture and comprises a liquid dropping mechanism, a liquid dropping tray, a moving mechanism and a base plate, the liquid dropping mechanism is mounted on the base plate, the moving mechanism is mounted on the base plate and positioned below the liquid dropping mechanism, the liquid dropping tray is mounted on the moving mechanism, and the moving mechanism is positioned below the liquid dropping tray. The liquid dropping disc is used for containing a plurality of independent containers, the movement direction of the liquid dropping mechanism is perpendicular to the movement direction of the moving mechanism, and the liquid dropping mechanism and the moving mechanism move in a crossed mode to ensure that quantitative strains are dropped into all the independent containers in the liquid dropping disc. According to the utility model, the small balls are prevented from absorbing moisture, shrinking or adhering together in the sub-packaging process, and the beneficial effects of short sub-packaging time and convenience in sub-packaging are achieved.

Description

TECHNICAL FIELD

[0001] The utility model provides quantitative strain drop ball device relates to microbiological culture technical field. BACKGROUND

[0002] Quantitative strain refers to in the biological product production process, for detecting and controlling the specific strain of product quality, using liquid nitrogen freeze drying technology mixes certain concentration range's specific strain with special protection agent according to certain proportion, drops into liquid nitrogen, makes the bacterial solution freeze rapidly under the ultra low temperature environment, forms spherical, and the strain enters dormancy state. Then divides and packs in the independent container, generally divides and packs in the test tube. Each test tube divides and packs one quantitative strain ball, adds rubber plug, extracts vacuum, presses plug, adds aluminium cover.

[0003] Because quantitative strain is freeze dried and then divides and packs, the phenomenon of moisture absorption and shrinkage is easy to appear in the dividing and packing process, the small ball after moisture absorption is easy to adhere to the dividing and packing tool or adhere to each other, which affects the dividing and packing effect. Because the dividing and packing process is mostly manual operation, the time is long, and the vacuum sealing cannot be quickly carried out after the dividing and packing, which affects the stability of the bacterial count. UTILITY MODEL CONTENT

[0004] The utility model provides quantitative strain drop ball device, solves the phenomenon that the quantitative strain ball is easy to appear moisture absorption and shrinkage after freeze drying in prior art, and the technical problem that manual operation divides and packs for a long time, and affects the stability of bacterial count.

[0005] The utility model is such a realization that including drop mechanism, drip tray, moving mechanism, control system and bottom disc, the drop mechanism is installed on the bottom disc, the moving mechanism is installed on the bottom disc and is located below the drop mechanism, the drip tray is used for holding a plurality of independent containers and is installed on the moving mechanism, the movement direction of the drop mechanism is perpendicular with the movement direction of the moving mechanism, the control system is installed on the bottom disc and is located one side of the drop mechanism, controls the crosswise intersection movement of the drop mechanism and the moving mechanism, and drops liquid to the independent container.

[0006] As further preferred, the drop mechanism slides left and right, and the moving mechanism slides forward and backward.

[0007] As further preferred, the drop mechanism slides forward and backward, and the moving mechanism slides left and right.

[0008] As a further preferred, the droplet mechanism comprises a droplet rack, a connecting plate, a droplet rod, a droplet plate, an anti-freezing plate, a first sliding mechanism and a micro peristaltic pump, the droplet rack is installed on the bottom plate, the first sliding mechanism is installed on one side of the droplet rack, the connecting plate is installed on the side of the first sliding mechanism, the droplet rod, the droplet plate and the anti-freezing plate are installed on the connecting plate from top to bottom in turn, the droplet rod is provided with a droplet through hole penetrating through the whole droplet rod, the droplet plate is provided with a liquid falling hole corresponding to the lower end of the droplet through hole, the anti-freezing plate is installed on the bottom surface of the droplet plate, the anti-freezing plate is provided with the liquid falling hole, and the micro peristaltic pump is installed on one side of the bottom plate and communicates with the upper end of the droplet rod.

[0009] As a further preferred, the droplet mechanism comprises an anti-freezing plate, the anti-freezing plate is installed on the bottom of the droplet plate, and the anti-freezing plate is provided with a liquid falling hole.

[0010] As a further preferred, the droplet disc comprises a disc body, a first hole rack, a second hole rack and a top plate, the first hole rack is installed in the disc body, the second hole rack is installed on the first hole rack, and the top plate is installed above the second hole rack, the first hole rack, the second hole rack and the top plate are all provided with bottle holes matched with the diameter of the independent container, the bottle holes in the range of the first hole rack, the bottle holes in the range of the second hole rack and the bottle holes on the top plate correspond to each other.

[0011] As a further preferred, a liquid leakage hole is arranged between adjacent bottle holes.

[0012] As a further preferred, the moving mechanism comprises a second sliding mechanism and a droplet disc support, the second sliding mechanism is installed on the bottom plate, the droplet disc support is installed on the second sliding mechanism, and the droplet disc is installed on the droplet disc support.

[0013] As a further preferred, the first sliding mechanism and the second sliding mechanism are both linear module sliding mechanisms.

[0014] As a further preferred, a plurality of positioning shafts are arranged at the position close to the side of the droplet disc support.

[0015] The utility model discloses a working principle, the first hole frame, second hole frame are loaded in the drop plate, put the independent container into the bottle hole of first hole frame, second hole frame, then buckle the roof. Place the drop plate in liquid nitrogen, make the drop plate enter liquid nitrogen, and the independent container is in liquid nitrogen atmosphere, and it is beneficial to drop liquid into ball. The drop plate is clamped on the drop plate support with the clamp, and the quantitative strain drop ball device is opened, the drop mechanism and the moving mechanism are in the initial position, the drop mechanism is aligned with the opening of the independent container in the first row first column / the first row last column on the left of the first hole frame, the trace peristaltic pump is opened, and the bacteria liquid is pumped into the drop mechanism, and the drop mechanism starts to drop liquid drop by drop, and a time interval is arranged between each drop, and the drop mechanism gradually moves backward / forward after every drop, and the distance of each movement is equivalent to the distance between the adjacent independent containers, and after moving once, the sensing head senses the empty container, and then the trace peristaltic pump and the drop rod drop a drop of liquid, and after moving the distance between the adjacent independent containers, the empty container is sensed again, and the liquid is dropped again, until the bacteria liquid is dropped into every independent container in the first row. After the independent container in the first row is filled with the bacteria liquid, the moving mechanism drives the drop plate to move left / right, and the drop mechanism fills the bacteria liquid into the independent container in the second row one by one. The bacteria liquid is filled into all the independent containers by such reciprocating. The second operation scheme can also be provided, the drop mechanism starts to align with the opening of the independent container in the first row first column / the first row last column, and after the bacteria liquid is dropped, it starts to prepare to move left / right, the moving mechanism moves forward / backward, the drop mechanism and the moving mechanism cross cooperation, and drop liquid. Since the drop plate is in liquid nitrogen atmosphere, the bacteria liquid dropped by the drop mechanism quickly forms spherical shape and floats in the independent container. After all the independent containers are filled with the bacteria liquid ball, the two sides of the drop plate are clamped with the clamp, and the drop plate is taken off from the drop plate, the rubber plug is covered, the rubber plug is not completely pressed down, and the gap is left, and then the drop plate is put into the freeze dryer for vacuum drying.

[0016] The utility model discloses the beneficial effect, utilize trace peristaltic pump and drop mechanism, moving mechanism cooperation, directly drop into the bacteria liquid in independent container, directly freeze -drying and form quantitative strain small ball, do not need to again sub -packaging, avoid the small ball sub -packaging process, hygroscopic, shrink or stick together. Independent container can directly press the lid under the vacuum state, guarantee the stability of the bacteria number. Through the cooperation of drop mechanism and moving mechanism, the bacteria liquid is dropped into the independent container placed in the drop plate, and the small ball is formed, which saves manpower and is convenient to operate. Prevent the liquid nitrogen from continuously evaporating upward during the filling process, and the drop needle is close to the bottle mouth, which may cause the temperature of the needle head to be too low, resulting in the change of the drop amount, and even the blockage of the needle head. A frost plate is arranged below the needle head, a first drop hole is arranged at the center of the frost plate, the first drop hole is arranged directly below the drop rod, and only one drop can pass through the first drop hole. In this way, the needle head of the drop rod will not be blocked due to the low temperature during the entire drop process, ensuring the constant drop amount of each drop, and further reducing the deviation of the bacteria number in the drop. ACCURATE

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings described below are only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained from these drawings without creative labor.

[0018] Figure 1 is a schematic diagram of the overall structure of an embodiment of the present application;

[0019] Figure 2 is a schematic diagram of the composition of the drop plate of an embodiment of the present application;

[0020] Figure 3 is a top view of the drop plate of an embodiment of the present application when not placed in a separate container;

[0021] Figure 4 is a state diagram of the drop plate of an embodiment of the present application when placed in a separate container;

[0022] Figure 5 is a schematic diagram of the structure of the first sliding mechanism of an embodiment of the present application;

[0023] Figure 6 is a schematic diagram of the structure of the second sliding mechanism of an embodiment of the present application;

[0024] Figure 7 is a schematic diagram of the connection of the drop plate support and the second sliding mechanism of an embodiment of the present application

[0025] Figure 8 is a schematic diagram of the overall structure of an embodiment of the present application.

[0026] In the figure, 1 is a drop mechanism; 2 is a drop plate; 3 is a moving mechanism; 4 is a separate container; 5 is a drop rack; 6 is a drop rod; 7 is an anti-freezing plate; 8 is a first sliding mechanism; 9 is a plate body; 10 is a first hole rack; 11 is a second hole rack; 12 is a top plate; 13 is a bottle hole; 14 is a liquid leakage hole; 15 is a second sliding mechanism; 16 is a drop plate support; 17 is a positioning shaft; 18 is a linear module; 19 is an original point photoelectric; 20 is a rubber plug; 21 is an aperture; 22 is an outer shell; 23 is a bottom plate; 24 is a control system; 25 is a counter; 26 is a liquid falling hole; 27 is a micro-ceramic pump; 28 is a connecting plate. DETAILED DESCRIPTION

[0027] The quantitative strain drop ball device comprises a drop mechanism, a drop tray, a moving mechanism, a control system and a base plate, the drop mechanism is installed on the base plate, the moving mechanism is installed on the base plate and below the drop mechanism, the drop tray is used for containing a plurality of independent containers and is installed on the moving mechanism, the moving direction of the drop mechanism is perpendicular to the moving direction of the moving mechanism, the control system is installed on the base plate and on one side of the drop mechanism, and the control system controls the cross movement of the drop mechanism and the moving mechanism to drop liquid into the independent containers. Further, when the drop mechanism moves forward and backward, the moving mechanism moves left and right, if the drop mechanism moves left and right, the moving mechanism moves forward and backward, and the drop mechanism and the moving mechanism move in cooperation, so that all the independent containers are dropped into the bacterial liquid. During the operation of the quantitative strain drop ball device, the device is always in a liquid nitrogen atmosphere, so as to ensure that the bacterial liquid dropped into the independent containers is quickly freeze-dried to form quantitative strain small balls. Further, the independent containers are preferably penicillin bottles.

[0028] As a further preferred, the drop mechanism comprises a drop frame, a connecting plate, a drop rod, a drop plate, a first sliding mechanism and a micro peristaltic pump, the drop frame is installed on the base plate, the first sliding mechanism is installed on one side of the drop frame, the connecting plate is installed on the side of the first sliding mechanism, the drop rod and the drop plate are installed on the connecting plate in sequence from top to bottom, the drop rod is provided with a drop hole passing through the whole drop rod, the drop plate is installed below the drop rod, the drop plate is provided with a liquid falling hole corresponding to the lower end of the drop hole, and the micro peristaltic pump is installed on one side of the base plate and in communication with the upper end of the drop hole. The micro peristaltic pump can control the discharge amount of the bacterial liquid, and set the drop amount of the bacterial liquid to be one drop each time. The bacterial liquid pumped out by the micro peristaltic pump enters the drop hole from the upper end of the drop hole of the drop rod through a hose or other connection mode, and is dropped vertically downward along the drop rod.

[0029] Further, the drop mechanism comprises an anti-freezing plate, the anti-freezing plate is installed at the bottom of the drop plate, and the anti-freezing plate is provided with a liquid falling hole. The drop plate and the liquid falling hole of the anti-freezing plate are corresponding. The bacterial liquid falls into the independent containers in sequence along the liquid falling holes on the drop plate and the anti-freezing plate. In order to facilitate counting, the drop plate is selected to be a window type drop counting device, and the window of the window type drop counting device serves as the liquid falling hole and can accommodate the bacterial liquid to pass through.

[0030] As a further preferred, the drip tray comprises a tray body, a first hole frame, a second hole frame and a top plate, the tray body has four closed sides and an upper opening, the first hole frame is installed in the tray body, the second hole frame is installed on the first hole frame, and the top plate is installed above the second hole frame, the first hole frame, the second hole frame and the top plate are all provided with bottle holes matched with the diameter of the independent container, the bottle holes in the range of the first hole frame, the bottle holes in the range of the second hole frame and the bottle holes on the top plate correspond to each other. The bottle holes on the first hole frame, the second hole frame and the top plate are arranged in a square number. More specifically, the bottom of the first hole frame can be installed with legs or without legs, and the bottom of the second hole frame is installed with legs. There is a certain spacing between the frame body of the second hole frame and the frame body of the first hole frame, which can accommodate the bottle body of the independent container and also accommodate liquid nitrogen.

[0031] Further, a liquid leakage hole is provided between adjacent bottle holes, which facilitates the injection of liquid nitrogen into the tray body and increases the full contact between the liquid nitrogen in the drip tray and the independent container in the tray body. Further, the aperture of the liquid leakage hole is smaller than the aperture of the bottle hole. Further, the side of the drip tray extends horizontally outward to form a clamping plate, which facilitates clamping the drip tray. Further, the independent container is preferably a vial. The overall height of the first hole frame and the second hole frame matches the height of the vial body. The vial placed in the tray body has its bottom contacting the bottom surface of the tray body, and the neck of the vial is flush with the top plate, which is beneficial to keep the bottle stable and avoid tilting of the bottle due to slight shaking, affecting the accuracy of the drip.

[0032] As a further preferred, the moving mechanism comprises a second sliding mechanism and a drip tray holder, the second sliding mechanism is installed on the bottom tray, the drip tray holder is installed on the second sliding mechanism, and the drip tray is installed on the drip tray holder. Further, a baffle is installed around the drip tray holder to prevent the drip tray from sliding and affecting the accuracy of the drip. Other anti-slip cooperation can also be used to prevent the drip tray from moving. Further, a positioning shaft is provided near the side of the drip tray holder, when the drip tray is placed on the drip tray holder, the drip tray is located within the positioning range of the positioning shaft, and the positioning shaft is located outside the drip tray to define the drip tray.

[0033] As a further preferred, the first sliding mechanism and the second sliding mechanism are both linear module sliding mechanisms. The linear module sliding mechanism comprises a guide rail, a sliding table, a screw rod and a driving device. More specifically, the connecting plate is connected with the sliding table of the first sliding mechanism, the connecting plate is in a vertical state, and the first sliding mechanism drives the droplet plate to reciprocate in a linear range. The droplet disc holder is connected with the sliding table of the second sliding mechanism, and the droplet disc holder and the second sliding mechanism are both in a horizontal state, and the second sliding mechanism drives the droplet disc holder to reciprocate in a linear range.

[0034] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model. Embodiment

[0035] Referring to Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 and Figure 7 , the quantitative strain droplet device comprises a droplet mechanism 1, a droplet disc 2, a moving mechanism 3, a control system 24 and a base plate 23. The droplet mechanism 1 is installed on the base plate 23 and located at the rear of the base plate 23. The moving mechanism 3 is installed on the base plate 23 and located below the droplet mechanism 1. The droplet mechanism 1 and the moving mechanism 3 can perform linear reciprocating motion, and the movement direction of the droplet mechanism 1 and the movement direction of the moving mechanism 3 are perpendicular to each other. In this embodiment, the droplet mechanism 1 moves left and right, and the moving mechanism 3 moves forward and backward. The droplet disc 2 is installed on the moving mechanism 3. The droplet disc 2 is used for containing and fixing a plurality of independent containers 4, and the independent containers 4 are arranged in a square array. The droplet mechanism 1 and the moving mechanism 3 cross each other to move and drip bacterial liquid into the independent containers 4. In this embodiment, the independent containers 4 are selected to be test tubes. The control system 24 is installed on the base plate 23 and located on one side of the droplet mechanism 1 and the moving mechanism 3. The installation position of the control system 24 does not coincide with the installation position of the droplet mechanism 1 and the moving mechanism 3. The control system 24 has an operation screen for controlling the droplet mechanism 1 and the moving mechanism 3. The operation screen has keys for controlling the droplet mechanism 1 and the moving mechanism 3, power control keys, start / pause keys and other related keys.

[0036] Referring toFigure 1 and Figure 7 The drop mechanism 1 includes a drop frame 5, a drop rod 6, a counter 25, an anti-freezing plate 7, a connecting plate 28, a first sliding mechanism 8 and a micro peristaltic pump 27. The drop frame 5 is vertically installed on the chassis 23, and has a door shape as a whole, including a horizontal rod and vertical rods, the horizontal rod is installed between the two vertical rods, the vertical rods are vertically installed on the chassis 23, and the horizontal rod is parallel to the chassis 23. The first sliding mechanism 8 is installed on the drop frame 5, and further installed on the side of the horizontal rod, the length extension direction of the first sliding mechanism 8 is consistent with the length extension direction of the horizontal rod. Referring to Figure 5 The first sliding mechanism 8 is a linear module sliding mechanism, including a linear module 18 and an original point photoelectricity 19, the linear module 18 includes a guide rail, a sliding table, a screw rod and a driving device. The connecting plate 28 is connected with the sliding table, the drop rod 6, the counter 25 and the anti-freezing plate 7 are sequentially installed on the connecting plate 28 from top to bottom, the anti-freezing plate 7 is installed at the bottom of the counter 25, and the first sliding mechanism 8 drives the connecting plate 28 to slide left and right. The drop rod 6 is provided with a drop hole (not shown), and the drop hole penetrates through the whole drop rod 6. The counter 25 selects a window type drop counter, the window of the window type drop counter is used as a drop hole 26 to accommodate the bacterial liquid to pass through, the anti-freezing plate 7 is also provided with a drop hole 26 corresponding to the drop rod 6, the window of the window type drop counter and the drop hole 26 of the anti-freezing plate 7 are in position correspondence and penetrate each other. The micro peristaltic pump 27 is installed on one side of the chassis 23 and connected with the upper end of the drop rod 6 through a hose, the bacterial liquid pumped by the micro peristaltic pump 27 enters the drop hole of the drop rod 6 through the hose. The side of the chassis 23 where the micro peristaltic pump 27 is installed can be determined according to the position of the drop mechanism 1. In the embodiment, at least one drop base is arranged between the drop rod 6 and the connecting plate 28, and two drop bases can be arranged, the two drop bases are provided with drop rod fixing holes (not shown), and the two drop bases are distributed in an up-down manner, the drop rod fixing holes of the two drop bases respectively fix the upper part and the lower part of the drop rod 6, so that the drop rod 6 is in a vertical state. The aperture of the drop rod fixing hole is matched with the diameter of the drop rod 6, the upper end and the lower end of the drop rod 6 respectively extend out of the drop base, and the upper end of the drop rod 6 is connected with the micro peristaltic pump 27 through a hose. Because the drop base is arranged, the drop rod 6 and the connecting plate 28 have a certain spacing, so that the drop rod 6 corresponds to the window position of the center of the counter 25.

[0037] Referring to Figure 1 , Figure 6 and Figure 7The moving mechanism 3 comprises a second sliding mechanism 15 and a drip tray holder 16, the drip tray holder 16 is installed on the second sliding mechanism 15, and the drip tray holder 16 is used for placing the drip tray 2. Referring to Figure 6 The second sliding mechanism 15 is a linear module sliding mechanism, comprising a linear module 18 and an original point photoelectricity 19. The linear module 18 comprises a guide rail, a sliding table, a screw rod and a driving device, and the drip tray holder 16 is installed on the sliding table of the second sliding mechanism 15. The second sliding mechanism 15 drives the drip tray holder 16 to move forward and backward, thereby driving the drip tray 2 to move left and right. A plurality of positioning shafts 17 are arranged at positions close to the side of the drip tray holder 16. The number of the positioning shafts 17 is six, two of the positioning shafts 17 are arranged on one side of the drip tray holder 16, and the remaining four positioning shafts 17 are arranged on the opposite side of the drip tray holder 16. An outer shell 22 is installed outside the base plate 23. The control system 24 is connected with the micro peristaltic pump 27, the first sliding mechanism 8, the second sliding mechanism 15 and the counter 25. The base plate 23 has four base feet.

[0038] Referring to Figure 1 , Figure 2 and Figure 3 The drip tray 2 comprises a tray body 9, a first hole frame 10, a second hole frame 11 and a top plate 12. The tray body 9 has a side plate and a bottom plate, the side plate and the bottom plate are provided with a closed plate without notches or through holes, and the side plate of the tray body extends horizontally outward to form a clamping plate, so that the tray body 9 can be clamped by a clamp. The first hole frame 10 is installed in the tray body 9 and is matched with the bottom surface of the tray body 9. The second hole frame 11 is installed on the first hole frame 10, that is, the second hole frame 11 is placed on the first hole frame 10, and the top plate is installed above the second hole frame 11. The first hole frame 10, the second hole frame 11 and the top plate 12 are all provided with bottle holes 13 matched with the diameter of the penicillin bottle. The first hole frame 10 can be matched with the bottom surface of the tray body 9 with or without a leg, the second hole frame 11 is installed on the first hole frame 10, and the second hole frame 11 has a leg. The first hole frame 10 and the second hole frame 11 can be taken out of or put into the tray body 9 as needed, and the top plate 12 is also a movable plate. After the penicillin bottles are installed in the first hole frame 10 and the second hole frame 11, the top plate 12 can be placed in the neck area of the penicillin bottles, and the top plate 12 is at the same horizontal plane as the upper end of the side plate of the tray body 9. A leakage hole 14 is arranged between adjacent bottle holes 13, and the aperture of the leakage hole 14 is smaller than the aperture of the bottle hole 13. Referring to Figure 4Before freeze-drying, a sterile rubber plug 20 is placed on the bottle mouth of the vial with a sterile clamp, the lower part of the plug 20 has a hole 21, when the plug 20 is placed on the bottle mouth of the vial, the hole 21 of the plug 20 is left to facilitate the air in the vial to be pumped out to form a vacuum state, after the freeze-drying is completed, the plug is directly pressed down by the machine under the vacuum state, then taken out and sealed with an aluminum cap to form the product. Embodiment

[0039] Reference Figure 8 The difference between Embodiment 2 and Embodiment 1 is that the sliding direction of the dripping mechanism 1 and the moving mechanism 3 is different, and the installation positions of the two on the base plate 23 are different. In this embodiment, the dripping mechanism 1 is vertically installed at the middle position of the base plate 23, the installation position is parallel to the short side of the base plate 23, the dripping mechanism 1 is installed close to the control system 24, the dripping mechanism 1 moves forward and backward, the sliding direction of the first sliding mechanism 8 is perpendicular to the operation screen of the control system 24. The second sliding mechanism 15 of the moving mechanism 3 slides left and right, the moving mechanism 3 is installed on the base plate 23 and located below the dripping mechanism 1, the installation position is perpendicular to the short side of the base plate 23, and the sliding direction of the second sliding mechanism 15 is parallel to the operation screen of the control system 24.

[0040] The utility model discloses a working principle, the first hole frame, second hole frame are loaded in the drop plate, put the plurality of independent container into the bottle hole of first hole frame, second hole frame, then buckle the top plate. Place the drop plate in liquid nitrogen, make the drop plate enter liquid nitrogen, and the independent container is in liquid nitrogen atmosphere, and it is beneficial to drop liquid into ball. The drop plate is clamped on the drop plate support with the clamp, and the quantitative strain drop ball device is opened, the drop mechanism and the moving mechanism are in the initial position, the drop mechanism is aligned with the opening of the independent container in the first row first column / the first row last column of the first hole frame left side, the micro peristaltic pump is opened, and the bacteria liquid is pumped into the drop mechanism, and the drop mechanism starts to drop liquid drop by drop, and time interval is arranged between each drop, and the drop mechanism gradually moves backward / forward after every drop, and the distance of each movement is equivalent to the distance between the adjacent independent containers, and after moving once, the sensing head senses the empty container, and then the micro peristaltic pump and the drop rod drop a drop of liquid, and after moving the distance between the adjacent independent containers, the empty container is sensed again, and the liquid is dropped again, and the bacteria liquid is dropped into every independent container in the first row. After the independent container in the first row is filled with the bacteria liquid, the moving mechanism drives the drop plate to move left / right, and the drop mechanism fills the bacteria liquid into the independent container in the second row one by one. The bacteria liquid is filled into all the independent containers by such reciprocating. The second operation scheme can also be provided, the drop mechanism starts to align with the opening of the independent container in the first row first column / the first row last column, and after the bacteria liquid is dropped, it starts to prepare to move left / right, and the moving mechanism moves forward / backward, and the drop mechanism and the moving mechanism cross cooperation, and drop liquid is completed. Since the drop plate is in liquid nitrogen atmosphere, the bacteria liquid dropped by the drop mechanism quickly forms spherical shape and floats in the independent container. After all the independent containers are filled with the bacteria liquid ball, the two sides of the drop plate are clamped with the clamp, and the drop plate is taken off from the drop plate, and the rubber plug is covered, and the rubber plug is not completely pressed down, and the gap is left, and then the drop plate is put into the freeze dryer for vacuum drying.

[0041] The utility model discloses the beneficial effect, directly drop the bacteria liquid into the independent container, directly freeze dry and form the quantitative strain small ball, do not need to pack again, avoid the small ball packing process to absorb moisture, shrink or stick together. The independent container can directly press the lid under the vacuum state, guarantee the stable bacteria number. Through the cooperation of drop mechanism and moving mechanism, drop the bacteria liquid into the independent container placed in the drop plate, form the small ball, save manpower, convenient operation. Prevent the liquid nitrogen from continuously evaporating upward during the filling process, and the drop needle is close to the bottle mouth, which may cause the temperature of the needle to be too low, resulting in the change of the drop amount, and even the blockage of the needle, and the anti-freezing plate is arranged below the needle, the first drop hole is arranged in the center of the anti-freezing plate, the first drop hole is arranged directly below the drop rod, and only one drop can pass through smoothly. In this way, the needle of the drop rod will not be blocked due to the low temperature during the entire drop process, ensuring the constant drop amount of each drop, and further reducing the deviation of the bacteria number in the drop.

[0042] The above merely describes preferred embodiments of the present application and is not intended to limit the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A quantitative strain droplet device, characterized in that, The device comprises a dripping mechanism, a dripping tray, a moving mechanism, a control system and a chassis, the dripping mechanism is installed on the chassis, the moving mechanism is installed on the chassis and below the dripping mechanism, the dripping tray is used for containing several independent containers and is installed on the moving mechanism, the moving direction of the dripping mechanism is perpendicular to the moving direction of the moving mechanism, the control system is installed on the chassis and on one side of the dripping mechanism, and the control system controls the cross movement of the dripping mechanism and the moving mechanism to drip liquid into the independent containers.

2. The quantitative strain droplet device of claim 1, wherein, The dripping mechanism slides left and right, and the moving mechanism slides forward and backward.

3. The quantitative strain droplet device of claim 1, wherein, The dripping mechanism slides forward and backward, and the moving mechanism slides left and right.

4. The quantitative strain droplet device of claim 2 or 3, wherein, The dripping mechanism comprises a dripping frame, a connecting plate, a dripping rod, a dripping plate, a first sliding mechanism and a micro peristaltic pump, the dripping frame is installed on the chassis, the first sliding mechanism is installed on one side of the dripping frame, the connecting plate is installed on the side of the first sliding mechanism, the dripping rod, the dripping plate and the anti-freezing plate are sequentially installed on the connecting plate from top to bottom, the dripping rod is provided with a dripping through hole penetrating through the whole dripping rod, the dripping plate is provided with a liquid falling hole corresponding to the lower end of the dripping through hole, and the micro peristaltic pump is installed on one side of the chassis and communicates with the upper end of the dripping rod.

5. The quantitative strain droplet device of claim 4, wherein, The dripping mechanism comprises an anti-freezing plate, the anti-freezing plate is installed at the bottom of the dripping plate, and the anti-freezing plate is provided with a liquid falling hole.

6. The quantitative strain droplet device of claim 4, wherein, The dripping tray comprises a tray body, a first hole frame, a second hole frame and a top plate, the first hole frame is installed in the tray body, the second hole frame is installed on the first hole frame, and the top plate is installed above the second hole frame, the first hole frame, the second hole frame and the top plate are all provided with bottle holes matched with the diameter of the independent containers, the bottle holes in the range of the first hole frame, the bottle holes in the range of the second hole frame and the bottle holes on the top plate correspond to each other.

7. The quantitative strain droplet device of claim 6, wherein, Liquid leakage holes are arranged between adjacent bottle holes.

8. The quantitative strain droplet device of claim 6, wherein, The moving mechanism comprises a second sliding mechanism and a dripping tray support, the second sliding mechanism is installed on the chassis, and the dripping tray support is installed on the second sliding mechanism and the dripping tray.

9. The quantitative strain droplet device of claim 8, wherein, The first sliding mechanism and the second sliding mechanism are both linear module sliding mechanisms.

10. The quantitative strain droplet device of claim 9, wherein, A plurality of positioning shafts are arranged at the position close to the edge side of the dripping tray support.

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