Culture dish device for laboratory bacteriostatic activity test

Abstract

The utility model belongs to the technical field of experimental utensils, and discloses a culture dish device for laboratory bacteriostatic activity tests, a perforated plate is mounted in a culture dish base in a penetrating manner, and a culture dish cover is placed on the top of the perforated plate; seven hole forming columns are fixedly installed at the bottom of the punching plate, and two symmetrically-arranged S-shaped components are fixedly installed on the outer side of the punching plate. The perforated plate can be effectively separated by adopting the S-shaped component, so that the solidified culture medium forms uniform sample adding holes, and meanwhile, the pollution risk of the culture medium is reduced; the fixed clamping seat and the positioning groove are adopted to effectively position the position of the sample adding hole to be concentric with the positioning circle, direct measurement and comparison of test effects are facilitated, compared with a traditional puncher, the device is easy to operate, the defects of cracking of a solid culture medium and the like are not easily caused, and the environmental microbial pollution risk in the bacteriostatic experiment process is effectively controlled; and the diameter of the inhibition zone can be directly measured.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of microorganism and medical experiment vessel, concretely to a petri dish device for laboratory bacteriostatic activity test. BACKGROUND

[0002] In the traditional bacteriostatic activity experiment, the Oxford cup method and the punching method are common bacteriostatic experimental methods. The Oxford cup method first pours a small amount of melted solid culture medium into a culture dish as a bottom layer of culture medium, places a sterile Oxford cup after it solidifies, then pours part of the culture medium again, removes the Oxford cup after it solidifies to form a sample addition hole, or directly pours a small amount of melted solid culture medium into a culture dish, waits for it to solidify, and then adds bacteriostatic active substances in the Oxford cup for experiment. The punching method directly pours melted solid culture medium, uses sterilized punching equipment to punch holes to form sample addition holes after it solidifies. The Oxford cup method is complicated and time-consuming, and the Oxford cup is prone to uneven placement, collapse, and other problems when placed, and is prone to tipping over when pouring in the culture medium. Using a punching device to punch holes requires sterilizing and drying the puncher in advance, which may not be effective and may contaminate the culture medium. In addition, the solid culture medium may break during the punching operation, and the culture medium fragments in the punched holes are difficult to clean, resulting in uneven penetration of bacteriostatic substances and irregular bacteriostatic rings, which affects the observation and measurement results.

[0003] In the prior art, document CN217265705U discloses a new environmental protection experiment bacteriostatic device, which is provided with two petri dish covers, one of which is connected with four cylinders, and the other is a normal petri dish cover. This punching method has a uniform punching depth and hole diameter, but also has certain defects. During the punching process, the punching cover with the cylinders needs to be removed from the petri dish, and then another petri dish cover needs to be replaced immediately. During the replacement of the petri dish cover, the microorganisms in the air may settle and contaminate the culture medium, affecting the results of the bacteriostatic experiment. For example, the Chinese utility model patent with publication number CN209872946U discloses a special petri dish for bacteriostatic experiment, which is provided with a fixed column on the cover, and a punching column that can be inserted into the fixed column. This method has certain defects, as the punching column needs to be removed by the operator during the operation, which may contaminate the culture medium when the petri dish is used in large quantities. UTILITY MODEL CONTENTS

[0004] To overcome the shortcomings of the prior art, the utility model provides a petri dish device for laboratory bacteriostatic activity test, which has the advantages of simple operation, uniform distribution of sample addition holes at the same level, reduced contamination when removing the punching plate after forming the sample addition holes, and direct evaluation of bacteriostasis, solving the above problems.

[0005] In order to realize the above-mentioned operation simple, both can make the sample hole position distribution uniform and be in the same horizontal plane, also can take away the punch plate after forming the sample hole, reduce the pollution, also can directly evaluate the purpose of bacteriostasis, the utility model provides the following technical scheme: a culture dish device for laboratory bacteriostatic activity test, including culture dish cover, punch plate and culture dish base, the punch plate is inserted in the inside of culture dish base, the culture dish cover is placed on the top of punch plate, the bottom of punch plate is fixedly installed with seven hole forming columns, the top of punch plate is fixedly installed with pickup structure, the outside of punch plate is fixedly installed with two symmetrically arranged S-shaped parts.

[0006] Preferably, the open edge of the culture dish base is provided with a positioning groove corresponding to the S-shaped part, the S-shaped part is clamped in the positioning groove, the inner wall of the culture dish base is fixedly installed with a fixed clamping seat, and the bottom of the culture dish base is provided with seven positioning circles and scale lines.

[0007] Preferably, one of the hole forming columns is installed at the center of the punch plate, and the remaining six are arranged in a ring shape at equal intervals, and the center of the hole forming column is consistent with the center of the positioning circle.

[0008] Preferably, the pickup structure is a conical or columnar body.

[0009] Preferably, the hole forming column is vertically arranged with the bottom of the punch plate, and when the punch plate is combined with the culture dish base, a gap is left between the bottom of the hole forming column and the inner bottom wall of the culture dish base.

[0010] Preferably, the top end of the S-shaped part is provided with a lower groove, and the S-shaped part is detachably assembled with the culture dish base through the lower groove and the positioning groove; the bottom end of the S-shaped part is provided with an upper groove, and the S-shaped part is detachably assembled with the culture dish cover through the upper groove.

[0011] Preferably, the number of fixed clamping seats is several, which are arranged in a ring shape horizontally, the fixed clamping seat is wedge-shaped, and the bottom of the punch plate is placed on the fixed clamping seat.

[0012] Compared with the prior art, the utility model has the following beneficial effects: the culture dish of the utility model is provided with an embedded punch plate, which can effectively control the distribution and size of the sample hole, reduce the risk of microbial contamination caused by punching the culture medium with a punching device, adopt S-shaped parts to effectively separate the punch plate, make the solidified culture medium form uniform sample holes, and reduce the risk of culture medium contamination; adopt fixed clamping seats and positioning grooves to effectively position the sample hole and the positioning circle at the same center, facilitate direct measurement and comparison of test results, relative to the traditional puncher, the device is simple to operate and is not easy to cause defects such as cracking of solid culture medium, effectively controls the risk of environmental microbial contamination in the bacteriostatic experiment process, and can directly measure the diameter of the bacteriostatic circle. Attached Figure Description

[0013] Figure 1 This is an exploded three-dimensional structural diagram of the present invention;

[0014] Figure 2 This is a two-dimensional wireframe schematic diagram of the combined structure of this utility model;

[0015] Figure 3 This is a schematic diagram of the isometric cross-section of the combined structure of this utility model from the southwest.

[0016] Figure 4 This is a schematic diagram of the cross-sectional structure of the perforated plate of this utility model;

[0017] Figure 5 This is a schematic diagram of the petri dish base structure of this utility model.

[0018] In the diagram: 1. Petri dish lid; 2. Perforated plate; 3. Petri dish base; 4. Hole forming column; 5. Pick-up structure; 6. Lower groove; 7. Upper groove; 8. Fixing bracket; 9. Positioning groove; 10. Positioning circle; 11. Scale line; 12. S-shaped component. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Please see Figures 1-2 A petri dish apparatus for laboratory antibacterial activity testing includes a petri dish lid 1, a perforated plate 2, and a petri dish base 3. The petri dish lid 1, perforated plate 2, and petri dish base 3 are all made of transparent acrylic, although the perforated plate 2 is not limited to transparent acrylic. The perforated plate 2 is inserted into the interior of the petri dish base 3, and the petri dish lid 1 is placed on top of the perforated plate 2.

[0021] Please see Figures 2-4 The bottom of the perforated plate 2 is fixedly equipped with seven hole-forming columns 4. One of the hole-forming columns 4 is installed at the center of the perforated plate 2, and the other six are arranged in a ring with equal spacing. The hole-forming columns 4 are perpendicular to the bottom of the perforated plate 2. When the perforated plate 2 is combined with the petri dish base 3, there is a gap of 2mm between the bottom of the hole-forming column 4 and the inner bottom wall of the petri dish base 3 to facilitate the uniform diffusion of antibacterial substances in the sample well.

[0022] Please see Figures 2-4The top of the punch plate 2 is fixedly provided with a pickup structure 5 which is a conical or columnar body.

[0023] Please refer to Figures 2-4 The outer side of the punch plate 2 is fixedly provided with two symmetrically arranged S-shaped components 12, the top end of the S-shaped component 12 is provided with a lower groove 6, the lower groove 6 is convenient to engage with the positioning groove 9, the S-shaped component 12 is detachably assembled with the culture dish base 3 through the lower groove 6 and the positioning groove 9; the bottom end of the S-shaped component 12 is provided with an upper groove 7, the S-shaped component 12 is detachably assembled with the culture dish cover 1 through the upper groove 7. The upper groove 7 of the S-shaped component 12 supports the culture dish cover 1, which is convenient for the operator to take out the punch plate 2, thereby reducing the risk of microbial contamination caused by repeatedly replacing the culture dish cover and puncher in the experimental process.

[0024] Please refer to Figures 3-5 The open edge of the culture dish base 3 is provided with a positioning groove 9 corresponding to the S-shaped component 12, the depth of the positioning groove 9 is 0.8mm, and the width is 5.2mm; the S-shaped component 12 is clamped in the positioning groove 9, the inner wall of the culture dish base 3 is fixedly provided with a fixed clamping seat 8, the number of the fixed clamping seat 8 is several, arranged in a ring shape, the fixed clamping seat 8 is wedge-shaped, the bottom of the punch plate 2 is placed on the fixed clamping seat 8, which is convenient for the punch plate 2 to be stable in the culture dish base 3, and the hole forming column 4 is a certain gap distance from the bottom of the culture dish base 3.

[0025] Please refer to Figures 3-5 The bottom of the culture dish base 3 is provided with seven positioning circles 10 and scale lines 11, the center of the hole forming column 4 is consistent with the center of the positioning circle 10, the diameter of the positioning circle 10 is consistent with the diameter of the hole forming column 4, and the accuracy of the scale line 11 is 1mm, taking the center of the positioning circle 10 as the reading starting point, and the extension line of the scale line 11 passes through the center point of the bottom circle of the culture dish base 3.

[0026] In use, when the culture dish is combined, the punch plate 2 is stable on the fixed clamping seat 8 of the culture dish base 3, the lower groove 6 of the S-shaped component 12 engages with the positioning groove 9, which is convenient for the fixed hole forming column 4 and the center of the positioning circle 10 to be on the same vertical line and one-to-one correspondence, which is convenient for directly measuring the size of the inhibition zone through the scale line 11, and multiple hole forming columns 4 can directly compare the size of the inhibition ability of multiple groups of inhibition substances. When the culture dish is combined, the hole forming column 4 and the culture dish base 3 have a 2mm gap reserved, which is beneficial to the uniform diffusion of the inhibition substance in the sample hole.

[0027] The culture dish cover 1 is supported by the upper groove 7 of the S-shaped component 12, and is moved upward away from the culture dish base 3 to reduce microbial contamination caused by repeated separation of the culture dish cover 1, the punched plate 2 and the culture dish base 3; the melted solid culture medium is poured into the culture dish base 3, and the liquid level of the culture medium is lower than the fixed clamping seat 8; the culture dish cover 1 and the punched plate 2 are supported by the upper groove 7 of the S-shaped component 12 and combined with the culture dish base 3, and the position of the hole forming column 4 is fixed by the positioning groove 9 and the lower groove 6 and corresponds to the positioning circle 10 one by one;

[0028] The punched plate 2 is fixed by the fixed clamping seat 8 to keep the horizontal position; after the culture medium solidifies, the punched plate 2 and the culture dish cover 1 are separated from the solid culture medium in the culture dish base 3 at the same time by the upper groove 7 to form a sample hole; after the operator adds an antibacterial active substance to the sample hole, the punched plate 2 and the culture dish cover 1 are separated, and then the culture dish cover 1 is combined with the culture dish base 3 alone.

Claims

1. A petri dish apparatus for laboratory bacteriostatic activity testing comprising a petri dish lid (1), a punch plate (2) and a petri dish base (3), characterized in that: The punching plate (2) is inserted into the inside of the culture dish base (3), and the culture dish cover (1) is placed on the top of the punching plate (2); the bottom of the punching plate (2) is fixedly provided with seven hole forming columns (4), the top of the punching plate (2) is fixedly provided with a picking structure (5), and the outer side of the punching plate (2) is fixedly provided with two symmetrically arranged S-shaped components (12).

2. A petri dish apparatus for use in laboratory bacteriostatic activity testing according to claim 1, characterized in that: The open edge of the culture dish base (3) is provided with a positioning groove (9) corresponding to the S-shaped component (12), the S-shaped component (12) is clamped in the positioning groove (9), and the inner wall of the culture dish base (3) is fixedly provided with a fixed clamping seat (8). The bottom of the culture dish base (3) is provided with seven positioning circles (10) and scale lines (11).

3. A petri dish apparatus for use in laboratory bacteriostatic activity testing according to claim 2, wherein: One of the hole forming columns (4) is arranged at the center of the punching plate (2), and the other six are arranged in a ring shape at equal intervals, and the center of the hole forming column (4) is consistent with the center of the positioning circle (10).

4. The petri dish apparatus for use in laboratory bacteriostatic activity testing of claim 1, wherein: The picking structure (5) is a conical or columnar body.

5. The petri dish apparatus for use in laboratory bacteriostatic activity testing of claim 1, wherein: The hole forming column (4) is vertically arranged with the bottom of the punching plate (2); when the punching plate (2) is combined with the culture dish base (3), the bottom of the hole forming column (4) and the inner bottom wall of the culture dish base (3) leave a gap.

6. A petri dish apparatus for use in laboratory bacteriostatic activity testing according to claim 2, wherein: The top end of the S-shaped component (12) is provided with a lower groove (6), and the S-shaped component (12) is detachably assembled with the culture dish base (3) through the lower groove (6) and the positioning groove (9); the bottom end of the S-shaped component (12) is provided with an upper groove (7), and the S-shaped component (12) is detachably assembled with the culture dish cover (1) through the upper groove (7).

7. A petri dish apparatus for use in laboratory bacteriostatic activity testing according to claim 2, wherein: The number of the fixed clamping seat (8) is several, which is arranged in a ring shape horizontally, the fixed clamping seat (8) is wedge-shaped, and the bottom of the punching plate (2) is placed on the fixed clamping seat (8).

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