Container box for immunohistochemical antigen repair

Abstract

The utility model discloses a container box for immunohistochemical antigen repair, which relates to the technical field of biomedicine and comprises a box body and a sealing cover, the box body is used for accommodating tissue slices and antigen repair liquid, and the sealing cover is connected to a top opening of the box body. A supporting frame used for supporting tissue slices is arranged in a cavity of the box body. The box body, the sealing cover and the supporting frame are all made of materials suitable for ultrasonic conduction. The device is reasonable in layout, compact in structure and convenient to use, the ultrasonic treatment requirement is met through material selection, the antigen repair efficiency and the dyeing quality can be effectively improved, the operation process is simplified, and the experiment cost and the reagent loss are reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biomedical technology, especially relate to a container box for immunohistochemical antigen repair. BACKGROUND

[0002] Immunohistochemistry (IHC) is a histopathological technique based on the principle of specific antigen-antibody binding, which can be used to locate, identify and quantify specific antigens in tissue sections. In the IHC experimental process, antigen retrieval is an indispensable key step, which aims to reverse the masking effect of epitopes caused by formalin fixation, so that the antibody can effectively bind to the target antigen on the tissue section, thereby improving the sensitivity of antigen detection and ensuring the reliability of the staining results.

[0003] Traditional antigen retrieval methods mainly include heat-induced epitope retrieval (HIER) and enzymatic epitope retrieval (EER). The HIER method usually uses water bath or pressure cooker heating, which is relatively complicated to operate and may cause the following problems: for example, bubbles on the surface of the tissue section cause uneven heat transfer, resulting in uneven antigen retrieval effect; high temperature or inappropriate retrieval conditions may cause damage to the morphological structure of the tissue, and even cause the tissue to fall off. EER method also has many potential problems that may affect the staining results: such as excessive enzyme digestion or improper operation, causing damage to the tissue structure, leading to the falling off of the section; non-specific proteolysis may occur during the enzyme digestion process, affecting the structure of other antigens other than the target antigen, resulting in false positive or false negative staining results; the activity difference of different batches of enzymes may reduce the repeatability of the experimental results and affect the stability of the experimental results.

[0004] Therefore, how to develop a container box for immunohistochemical antigen repair, which is suitable for the processing operation of an ultrasonic cleaner, ensures the uniformity of the section antigen retrieval effect and the reliability of the staining, has become a technical problem to be solved by the technical personnel in the field. UTILITY MODEL CONTENT

[0005] The utility model aims to provide a container box for immunohistochemical antigen repair, and a container box suitable for the processing operation of an ultrasonic cleaner, which solves the problems listed in the background art.

[0006] To solve the above technical problems, the utility model adopts the following technical scheme:

[0007] The utility model relates to a container box for immunohistochemical antigen repair, which comprises a box body for containing tissue slices and antigen repair solution and a sealing cover for preventing leakage, the sealing cover is connected to the top opening of the box body, a supporting frame for supporting the tissue slices is arranged in the cavity of the box body, and the box body, the sealing cover and the supporting frame are made of materials suitable for ultrasonic wave conduction.

[0008] Preferably, the symmetric edges of the supporting frame are limited by abutting against the inner side wall of the box body through the positioning part.

[0009] Preferably, the bottom edge of the supporting frame is inlaidly connected with the bottom surface of the box body.

[0010] Preferably, the box body, the supporting frame and the sealing cover are made of plastic or glass.

[0011] Preferably, the supporting frame is in the shape of a net or a grid.

[0012] Preferably, the supporting frame is a square frame, and the bottom of the two opposite side edges of the square frame is provided with a grid plate for the flow of antigen repair solution.

[0013] Preferably, the top of the supporting frame is an open grid plate, the bottom of the grid plate is fixedly connected with a closed connecting edge and the two are integrally formed, the closed connecting edge is inlaidly connected with the bottom surface of the box body, and a limiting clamping plate is arranged in the middle of the top of the grid plate along the length direction.

[0014] Preferably, when the box body is designed in a square shape, the sealing cover is clamped and positioned with the box body.

[0015] Preferably, when the box body is designed in a cylindrical shape, the sealing cover is clamped and positioned with the box body or is connected together through screwing.

[0016] Preferably, the bottom of the sealing cover is provided with a sealing strip matched with the opening top end of the box body, and the sealing strip is made of silica gel or rubber material.

[0017] Compared with the prior art, the utility model has the beneficial technical effects that:

[0018] The container box for immunohistochemical antigen repair is composed of a box body, a sealing cover and an internal supporting frame, in use, first, the tissue slices are placed in the supporting frame in the container box, then, an appropriate amount of antigen repair solution is added into the container box so as to completely cover the tissue slices, then, the sealing cover is connected to the opening to complete the sealing, finally, the container box is placed in an ultrasonic cleaning instrument for ultrasonic treatment, after the treatment, the tissue slices are taken out for subsequent immunohistochemical staining steps.

[0019] The utility model provides a container box for immunohistochemical antigen repair, through the selection of material satisfies the ultrasonic treatment demand, can effectively improve antigen repair efficiency and dyeing quality, and simplifies the operation process, reduces the experiment cost and reagent loss, the utility model has important application value, can be widely applied in pathological diagnosis, scientific research and other fields. BRIEF DESCRIPTION OF DRAWINGS

[0020] The utility model makes further explanation in combination with the drawings.

[0021] Figure 1 It is container box for immunohistochemical antigen repair of the utility model schematic diagram;

[0022] Figure 2 It is support frame structure one of the utility model schematic diagram;

[0023] Figure 3 It is support frame structure two of the utility model schematic diagram;

[0024] Figure 4 It is support frame structure three of the utility model schematic diagram;

[0025] Figure 5 It is use state schematic diagram of the utility model;

[0026] Mark the drawings 1, box body;2, support frame;3, grating plate;4, positioning portion;5, sealing cover;6, tissue slice;301, closed connection edge;302, limit stopper. DETAILED DESCRIPTION

[0027] In order to make the technical problem, technical scheme and beneficial effect that the utility model wants to solve more clear and obvious, the following combines the drawings and example, and further detailed explanation of the utility model is carried out.It should be understood that the specific example described here is only used to explain the utility model, and is not used to limit the utility model.

[0028] As Figures 1-5 Shown, a kind of container box for immunohistochemical antigen repair, including for containing tissue slice and antigen repair liquid box body 1 and the sealing cover 5 of leakage prevention, the sealing cover 5 is connected on the top opening of the box body 1;The cavity of the box body 1 is provided with support frame 2 for supporting tissue slice;The box body 1, sealing cover 5 and support frame 2 are all selected to be suitable for the material of ultrasonic transmission.

[0029] Specifically, a group of symmetrical edges of the support frame 2 is in abutment with the inner side wall of the box body 1 through the positioning portion 4 to form a limit stopper. Specifically, the box body 1 adopts a flat bottom, the distance between the support frame 2 and the inner side wall of the box body 1 is set to 15-30 mm, and the height of the support frame 2 is lower than the top surface of the box body 1, so that the sealing cover can be completely covered and sealed.

[0030] In one specific embodiment, the bottom edge of the support frame 2 is inlaidly connected with the bottom surface of the box body 1. Specifically, the bottom surface of the box body 1 is provided with a positioning groove, the shape and width of the positioning groove match the shape and thickness of the support frame 2, and the bottom edge of the support frame 2 is inlaidly connected into the positioning groove.

[0031] The box body 1, the support frame 2 and the sealing cover 5 are made of plastic or glass. Specifically, the box body 1 and the support frame 2 are made of the same material, preferably PMP material, and secondarily polycarbonate material or glass. The sealing cover 5 is preferably made of polypropylene material, and additionally polycarbonate material.

[0032] As shown in Figures 2-4 , the shape of the support frame 2 is set as a mesh or a grid.

[0033] As shown in Figure 2 , as an embodiment of the support frame, the support frame 2 is set as a square frame, and the bottom of two opposite sides of the square frame is provided with a grid plate 3 for flowing of the antigen repair solution. The bottom is designed in a communication structure, so that the antigen repair solution can flow freely in the box body, so as to quickly fill the box body and submerge the tissue slices.

[0034] As shown in Figure 3 , as another embodiment of the support frame, the bottom of two long sides of the square frame is provided with a grid plate structure, and the bottom of two short sides is provided with a mesh plate. In addition, the bottom of two short sides can also be designed as a closed flat plate.

[0035] As shown in Figure 4 , as a third embodiment of the support frame, the top of the support frame 2 is provided with an open grid plate 3, the bottom of the grid plate 3 is fixedly connected with a closed connecting edge 301, and the two are integrally formed. The closed connecting edge 301 is inlaidly connected on the bottom surface of the box body 1, and a limiting clamping plate 302 is arranged in the middle of the top of the grid plate 3 along the length direction, for limiting the tissue slices put in during the processing, preventing the tissue slices from floating upward, so that the tissue slices are completely soaked in the antigen repair solution, and the processing effect is ensured. Specifically, the limiting clamping plate 302 is designed as a "U" shape with an extension edge, the total length corresponds to the inner length of the box body 1, the opening length of the "U" shape corresponds to the length of the support frame 2, and the limiting clamping plate 302 is clamped and positioned.

[0036] In one specific embodiment, when the box body 1 is designed as a square, the sealing cover 5 is clamped and positioned with the box body 1.

[0037] In another embodiment, when the box 1 is designed as a cylinder, the sealing cover 5 is connected with the box 1 by clamping or screwing.

[0038] Specifically, the bottom of the sealing cover 5 is provided with a sealing strip matched with the open top end of the box 1, and the sealing strip is made of silica gel or rubber material. The sealing strip can further ensure the sealing performance after being closed.

[0039] As shown in Figure 5 The use process of the utility model is as follows:

[0040] Step one, place the tissue slices in the container box, first embed the support frame 2 into the bottom surface of the box 1, and then vertically insert the multiple tissue slices 6 into the gaps of the corresponding grid plates 3;

[0041] Step two, add an appropriate amount of antigen repair solution, and the antigen repair solution permeates through the grid plates 3 and fills the entire box 1 until the height of the antigen repair solution completely submerges the tissue slices 6;

[0042] Step three, ultrasonic treatment, first cover the sealing cover 5 to the top opening of the box 1 to form a seal, and then place the sealed container box in the ultrasonic cleaning instrument for ultrasonic treatment;

[0043] Step four, open the sealing cover and take out the tissue slices, and then clean the tissue slices with PBS buffer solution for three times, and then perform the subsequent immunohistochemical staining step.

[0044] Specifically, the ultrasonic treatment parameters are selected, the frequency of ultrasonic treatment is 20-100 kHz, the power is 50-200 W, and the treatment time is 5-30 minutes. Reasonable parameter settings are selected for different tissue slices.

[0045] Specifically, the antigen repair solution is a traditional repair solution such as citric acid and ethylenediaminetetraacetic acid (EDTA).

[0046] It should be noted that in this article, relationship terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between the entities or operations. Moreover, the terms "include", "contain" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or equipment.

[0047] The above-described embodiments are merely preferred modes of the present application, and are not intended to limit the scope of the present application, and various modifications and improvements to the technical solutions of the present application made by those of ordinary skill in the art without departing from the design spirit of the present application shall fall within the protection scope of the present application as defined by the claims.

Claims

1. A container cassette for immunohistochemistry antigen retrieval, characterized by: The box (1) for containing tissue slices and antigen repair liquid and the leak-proof sealing cover (5) connected to the top opening of the box (1). The box (1) is provided with a support frame (2) for supporting tissue slices in the cavity.

2. The container cartridge for immunohistochemistry antigen retrieval of claim 1, wherein: The symmetric edges of the support frame (2) are in abutment with the inner side wall of the box (1) through the positioning part (4) to form a limit.

3. The container cartridge for immunohistochemistry antigen retrieval of claim 1, wherein: The bottom edge of the support frame (2) is inlayed and connected with the bottom surface of the box (1).

4. The container cassette for immunohistochemistry antigen retrieval according to claim 1 or 2 or 3, characterized in that: The box (1), the support frame (2) and the sealing cover (5) are made of plastic or glass.

5. The container cartridge for immunohistochemistry antigen retrieval according to claim 1 or 2 or 3, characterized in that: The support frame (2) is in the shape of a net or a grid.

6. The container cartridge for immunohistochemistry antigen retrieval of claim 5, wherein: The support frame (2) is in the shape of a square frame, and the bottom of the two opposite sides of the square frame is provided with a grid plate (3) for the flow of antigen repair liquid.

7. The container cartridge for immunohistochemistry antigen retrieval of claim 5, wherein: The top of the support frame (2) is provided with an open grid plate (3), the bottom of which is fixedly connected with a closed connecting edge (301) and integrally formed, the closed connecting edge (301) is inlayed and connected with the bottom surface of the box (1), and the top of the grid plate (3) is provided with a limiting clamping plate (302) along the length direction.

8. The container cartridge for immunohistochemistry antigen retrieval of claim 1 or 2 or 3, wherein: When the box (1) is designed in a square shape, the sealing cover (5) is clamped and positioned with the box (1).

9. The container cartridge for immunohistochemistry antigen retrieval of claim 1 or 2 or 3, wherein: When the box (1) is designed in a cylindrical shape, the sealing cover (5) is clamped and positioned with the box (1) or is screwed and connected together.

10. The container cartridge for immunohistochemistry antigen retrieval of claim 1, wherein: The bottom of the sealing cover (5) is provided with a sealing strip matched with the opening top end of the box (1), and the sealing strip is made of silica gel or rubber material.

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