Biological sample oscillating grinding machine

Abstract

The utility model relates to the technical field of biochemical experiment automation equipment, and discloses a biological sample oscillation grinding machine which comprises a base and a support table installed on the base, a servo motor is installed on the base, a lifting seat is installed on the support table and arranged in a sliding bearing on the base through a guide column, and the servo motor is connected with the lifting seat. A crank wheel is connected to a rotating shaft of the servo motor, a through hole is formed in the middle of the support table, a connecting rod block is arranged below the lifting seat, the crank wheel and the connecting rod block are connected through a connecting rod to form a lifting mechanism, the lifting mechanism penetrates through the through hole in the support table, a sample fixing frame is installed on the lifting seat, and a container is arranged on the sample fixing frame. And a biological sample and crushed particles are placed in the container. The cell crusher is simple in structure and convenient to operate, driving parts are directly and uniformly controlled by an automatic system, and high-speed oscillation cell crushing is realized.

Description

TECHNICAL FIELD

[0001] The utility model relates to biochemical experiment automation equipment technical field especially relates to a biological sample oscillation abrasive machine. BACKGROUND

[0002] In the process of nucleic acid extraction experiment on biological samples, biological cells are broken to destroy the cell wall and cell membrane of the cells, so as to obtain the contents, DNA and nucleic acid components of the cells.

[0003] The current cell breaking methods mainly include ultrasonic method, oscillation method and chemical method. For example, the Chinese patent "frozen and thawed cell oscillation breaking instrument" (publication number: CN104762199A) discloses a method for breaking cells by shaking and impacting after freezing and thawing, and realizes vibration balance through two shaking arms in different directions. However, the device structure of this method is complex, the volume is large, it is not conducive to integration into an automatic system, the vibration frequency has a bottleneck, and the high-speed breaking effect cannot be achieved. Moreover, the breaking effect of the frozen and thawed method on plant cells is not ideal.

[0004] The commonly used vertical oscillation products of mechanical oscillation method are generally separate products, the structure is complex, and the products cannot be integrated into an automatic system. The power supply, control and other unit modules need to be provided separately. UTILITY MODEL CONTENT

[0005] The utility model aims at solving the above problems, and provides a biological sample oscillation abrasive machine, which is simple in structure, convenient to operate, and has driving components directly controlled by an automatic system to realize high-speed oscillation and cell breaking.

[0006] The utility model adopts the technical scheme of:

[0007] A biological sample oscillation abrasive machine, characterized by comprising a base and a support table installed on the base, a servo motor is installed on the base, a lifting seat is installed on the support table, the lifting seat is arranged in a sliding bearing on the base through a guide column, a crank wheel is connected to the rotating shaft of the servo motor, a through hole is formed in the middle of the support table, a connecting rod block is arranged below the lifting seat, the crank wheel and the connecting rod block are connected through a connecting rod to form a lifting mechanism, the lifting mechanism passes through the through hole on the support table, a sample fixing frame is installed on the lifting seat, a container is arranged on the sample fixing frame, and biological samples and breaking particles are placed in the container.

[0008] Further, the sample fixing frame is fixedly arranged on the lifting seat through a support column, a container rack is arranged on the sample fixing frame, a plurality of containers are arranged on the container rack, a pressing plate is arranged on the sample fixing frame, and the pressing plate presses the container and the container rack on the container rack through a pressure spring below the sample fixing frame.

[0009] Furthermore, the container rack is provided with multiple container cavities, each containing a container. The container rack is provided with positioning components, and the container rack and sample holder are positioned and fixed by the positioning components.

[0010] Furthermore, a lifting motor is installed on the support platform, and the lifting shaft of the lifting motor is located below the pressure plate. The lifting shaft of the lifting motor is used to lift the pressure plate open and then place or remove the container rack.

[0011] Furthermore, the container is a centrifuge tube, and the crushed particles are steel balls or quartz sand.

[0012] Furthermore, the sample holder is provided with a groove, the container holder is fitted into the groove, and the positioning component includes positioning holes provided on both sides of the container holder and positioning pins provided on both sides of the groove.

[0013] Furthermore, the pressure plate is provided with multiple pressure blocks, each pressure block corresponding to a container on the container rack.

[0014] Furthermore, the two ends of the pressure plate are mounted on the sample holder by two spring posts, and a pressure spring is sleeved on the spring post below the sample holder and locked to the lower end of the spring by a nut.

[0015] Furthermore, clearance holes are provided on the lifting frame below the container.

[0016] Furthermore, a positioning plate is provided on the lifting shaft, and a position sensor is provided on the lifting motor. The positioning plate and the sensor work together to control the lifting shaft's lifting limit.

[0017] The beneficial effects of this utility model are:

[0018] (1) High-speed vertical oscillation and breakage of centrifuge tubes are achieved by using a servo motor and crank connecting rod;

[0019] (2) Samples can be easily placed and removed simply by opening the pressure plate;

[0020] (3) It has a simple structure and is easy to operate, making it suitable for disrupting small batches of cell samples. Attached Figure Description

[0021] Appendix Figure 1 This is a three-dimensional structural schematic diagram of the present invention;

[0022] Appendix Figure 2 This is a three-dimensional structural schematic diagram of the present invention from another perspective;

[0023] Appendix Figure 3 This is an appendix to the utility model Figure 1 A magnified view of a portion of the scene from the perspective of the viewpoint;

[0024] Appendix Figure 4is the structure of the container rack Figure 2 is a local enlarged view of the perspective view upper part

[0025] is the structure of the container rack Figure 5 is the structure of the container rack

[0026] is the structure of the container rack Figure 6 is the structure of the container rack

[0027] is the structure of the container rack Figure 7 is the structure of the container rack

[0028] The reference signs in the drawings are as follows:

[0029] 1. base; 2. support table

[0030] 3. servo motor; 4. crank wheel

[0031] 5. lifting seat; 6. guide column

[0032] 7. sliding bearing; 8. connecting rod block

[0033] 9. connecting rod; 10. sample fixing frame

[0034] 11. container rack; 12. pressing plate

[0035] 13. centrifugal tube; 14. container cavity hole

[0036] 15. jacking motor; 16. jacking shaft

[0037] 17. pressing block; 18. spring column

[0038] 19. pressure spring; 20. nut

[0039] 21. positioning sheet; 22. position sensor DETAILED DESCRIPTION

[0040] The specific embodiments of the biological sample oscillation abrasive machine of the utility model will be described in detail below in combination with the drawings.

[0041] Referring to the drawings Figure 1 , 2 The biological sample oscillation abrasive machine of the patent comprises a base 1 for installing the equipment in an automatic system, and a support table 2 is installed on the base 1 through a support column. A servo motor 3 is installed on the base 1, a crank wheel 4 is connected to the rotating shaft of the servo motor 3, a lifting seat 5 is installed on the support table 2, and the lifting seat 5 is arranged in a sliding bearing 7 on the base 1 through a guide column 6.

[0042] The support table 2 is provided with a through hole in the middle, the lifting seat 5 is provided with a connecting rod block 8, the crank wheel 4 and the connecting rod block 8 are connected through a connecting rod 9 to form a lifting mechanism, the lifting mechanism passes through the through hole on the support table 2, and the two ends of the connecting rod 9 are connected with the crank wheel 4 and the connecting rod block 8 through rolling bearings. When the crank wheel rotates, the connecting rod 9 drives the lifting seat 5 to move up and down through the connecting rod block 8 due to the limitation of the guide column 6.

[0043] Referring to the accompanying drawings Figure 3 、 4 , 5, a sample fixing frame 10 is installed on the lifting seat 5, the sample fixing frame 10 is fixedly arranged on the lifting seat 5 through a support column, a container rack 11 is arranged on the sample fixing frame 10, a plurality of containers are arranged on the container rack 11, and a pressing plate 12 is arranged on the sample fixing frame 10. The container rack 11 is pressed on the container rack 11 by a pressure spring 19 below the sample fixing frame 10. Biological samples and broken particles are placed in the container.

[0044] The container in the accompanying drawings is a centrifugal tube, the broken particles are steel balls or quartz sand, a plurality of container cavities 14 are arranged on the container rack 11, one container is arranged in each container cavity 14, a positioning component is arranged on the container rack 11, and the container rack 11 is fixedly positioned with the sample fixing frame 10 through the positioning component. The sample fixing frame 10 is provided with a groove, the container rack 11 is clamped in the groove, and the positioning component comprises positioning holes arranged on both sides of the container rack 11 and positioning pins arranged on both sides of the groove.

[0045] A jacking motor 15 is installed on the support table 2, a jacking shaft 16 of the jacking motor 15 is located below the pressing plate 12, and an avoiding hole is arranged on the lifting seat 5 below the container to facilitate the movement of the jacking shaft 16. The jacking shaft 16 of the jacking motor 15 is used to take and place the container rack 11 after the pressing plate 12 is jacked open. A plurality of pressing blocks 17 are arranged on the pressing plate 12, and each pressing block 17 corresponds to a container on the container rack 11. The two ends of the pressing block 17 are arranged on the sample fixing frame 10 through two spring columns 18, the spring column 18 below the sample fixing frame 10 is sleeved with a pressure spring 19, and is locked at the lower end of the spring through a nut 20.

[0046] A positioning sheet 21 is arranged on the jacking shaft 16, a position sensor 22 is arranged on the jacking motor 15, and the positioning sheet 21 cooperates with the sensor to control the lifting limit of the jacking shaft 16. When the positioning sheet 21 and the position sensor 22 are inducted, the jacking motor 15 stops moving.

[0047] Referring to the accompanying drawings Figure 6 、 7The working process of the patent is as follows: firstly, the centrifuge tube 13 containing the animal and plant samples is prepared, the steel ball is placed in the centrifuge tube 13, the centrifuge tube 13 is arranged on the container rack 11, the jacking motor 15 is turned on, the container rack 11 is fixed in the groove of the sample fixing rack 10 after the pressing plate 12 is lifted, the jacking motor 15 drives the jacking shaft 16 to descend, and the pressing plate 12 presses the centrifuge tube 13 on the container rack 11. The servo motor 3 is started, the crank wheel 4 of the servo motor 3 drives the connecting rod 9 to make a rotary motion, the connecting rod 9 drives the connecting rod block 8 to make an up-down motion, the lifting seat 5 moves in the sliding bearing 7 to generate the lifting motion, and the motion of the lifting seat 5 makes the steel ball in the centrifuge tube 13 vibrate to break the animal and plant cells in the centrifuge tube 13. The rotating speed of the servo motor 3 can reach 7200 rpm, the high-speed rotation makes the steel ball vibrate in the centrifuge tube 13 at high speed, the animal and plant cells are broken, the internal substances of the cells are exposed, including the DNA substances in the cell nucleus, and necessary preparation is provided for subsequent nucleic acid extraction.

[0048] The above is only the preferred embodiment of the present application, and it should be pointed out that, for ordinary skilled in the art, without departing from the principles of the present application, a number of improvements and refinements can be made, and these improvements and refinements should be considered as the protection scope of the present application.

Claims

1. A biological sample oscillating mill, characterized by: The device comprises a base and a support platform installed on the base, a servo motor is installed on the base, a lifting seat is installed on the support platform, the lifting seat is arranged in the sliding bearing on the base through the guide column, the rotating shaft of the servo motor is connected with a crank wheel, a through hole is formed in the middle of the support platform, a connecting rod block is arranged below the lifting seat, the crank wheel and the connecting rod block are connected through a connecting rod to form a lifting mechanism, the lifting mechanism passes through the through hole on the support platform, a sample fixing frame is installed on the lifting seat, a container is arranged on the sample fixing frame, biological samples and broken particles are placed in the container.

2. The biological sample oscillation mill according to claim 1, characterized in that: The sample fixing frame is fixedly arranged on the lifting seat through a support column, a container rack is arranged on the sample fixing frame, a plurality of containers are arranged on the container rack, a pressing plate is arranged on the sample fixing frame, and the container and the container rack are pressed on the container rack by the pressure spring below the sample fixing frame.

3. The biological sample oscillation mill according to claim 2, characterized in that: A plurality of container cavities are arranged on the container rack, one container is arranged in each container cavity, and a positioning component is arranged on the container rack.

4. The biological sample shaking mill according to claim 3, characterized in that: A jacking motor is installed on the support platform, the jacking shaft of the jacking motor is located below the pressing plate, and the jacking shaft of the jacking motor is used to take and place the container rack after the pressing plate is jacked open.

5. The biological sample oscillation mill according to any one of claims 1 to 4, characterized in that: The container is a centrifugal tube, and the broken particles are steel balls or quartz sand.

6. The biological sample shaking mill according to claim 3, characterized in that: A groove is arranged on the sample fixing frame, the container rack is clamped in the groove, and the positioning component comprises positioning holes arranged on both sides of the container rack and positioning pins arranged on both sides of the groove.

7. The biological sample oscillation mill according to any one of claims 2 to 4, characterized in that: A plurality of pressing blocks are arranged on the pressing plate, and each pressing block corresponds to a container on the container rack.

8. The biological sample oscillation mill according to any one of claims 2 to 4, characterized in that: Both ends of the pressing plate are arranged on the sample fixing frame through two spring columns, a pressure spring is sleeved on the spring column below the sample fixing frame, and the spring is locked at the lower end through a nut.

9. The biological sample oscillation mill according to any one of claims 2 to 4, characterized in that: An avoiding hole is arranged below the lifting frame.

10. The biological sample shaking mill according to claim 4, characterized in that: A positioning sheet is arranged on the jacking shaft, a position sensor is arranged on the jacking motor, and the positioning sheet cooperates with the sensor to control the lifting and limiting of the jacking shaft.

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