Automatic cell drug sensitivity detection device

Abstract

The utility model relates to the technical field of biological medicine, in particular to an automatic cell drug sensitivity detection device which comprises a rack, a working table arranged on the rack, a transfer module, a chemiluminescence detection mechanism, a low-temperature storage box, a carbon dioxide incubator and a cell counter, and the working table is used for storing consumables and providing a working area for a drug sensitivity detection process; the chemiluminescence detection mechanism is used for drug sensitivity detection, the low-temperature storage box is used for storing experimental consumables, the carbon dioxide incubator is used for providing a suitable growth environment for organoid, and the cell counter is used for detecting the number of cells; the transferring module is used for adding drugs into the organoid, then putting the organoid culture microwell plate added with the drugs into a carbon dioxide incubator for culture, and after culture is completed, sending the organoid into a chemiluminescence detection mechanism for drug sensitivity detection; according to the device, the organoid drug sensitivity detection process can be automatically completed, manual operation intervention is not needed, experimental errors are reduced, and the working efficiency is greatly improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological medicine technical field especially, relate to a cell drug sensitivity detection automation device. BACKGROUND

[0002] The organoid technology is a new type of tissue organ in-vitro culture technology produced by introducing the self-assembly characteristics of stem cells into three-dimensional cell culture under the background of the continuous deepening of the research on mammalian development, tissue homeostasis and extracellular matrix and the increasing experience of stem cell culture, and the drug sensitivity detection experiment of the organoid is still in the laboratory stage, and errors are inevitable during manual operation culture, which is more obvious in the drug screening process, and even the drug detection results can be covered, so that the expected results cannot be achieved, and how to stably and reliably perform the organoid drug sensitivity detection has become a problem to be solved in the field. UTILITY MODEL CONTENT

[0003] The utility model discloses a cell drug sensitivity detection automation device, can complete the organoid drug sensitivity detection process automatically, and does not need manual operation intervention, reduces experimental error, and greatly improves work efficiency.

[0004] In order to realize the above-mentioned purpose, the utility model adopts the technical scheme: a cell drug sensitivity detection automation device, including frame, the workbench that sets up on the frame, removes and sends module, chemical luminescence detection mechanism, low temperature storage box, carbon dioxide incubator, cell counter, the workbench is used to store consumables and provides work area for drug sensitivity detection process, the chemical luminescence detection mechanism is used for drug sensitivity detection, the low temperature storage box is used for storing experimental consumables, the carbon dioxide incubator is used for providing the suitable growth environment of organoid, and the cell counter is used for detecting the number of cells, the removal and sending module are used to add the drug stored in the low temperature storage box to the organoid, then the organoid that has added the drug is cultured micro -well plate is put into carbon dioxide incubator and is cultivated, and after cultivation, the organoid is sent into chemical luminescence detection mechanism and is detected drug sensitivity.

[0005] The further improvement of the above scheme is that the chemical luminescence detection mechanism includes a chemiluminescence instrument, and a luminescent reagent stored in the low temperature storage box, the luminescent reagent is added to the drug detection micro -well plate through the removal and sending module, and the chemiluminescence instrument is used to detect the ATP content and analyze the results.

[0006] The further improvement of the above scheme is that the chemiluminescence instrument is an enzyme-labeled instrument.

[0007] Further improvement of the above scheme is that the low-temperature storage box and the carbon dioxide incubator are provided with a rotating stack module capable of storing micro-hole plates, the rotating stack mechanism comprises a stack base, a rotating platform rotatably arranged on the stack base, a rotating drive assembly arranged on the stack base, and a material placing rack arranged on the rotating platform, and the rotating drive assembly is drivingly connected with the rotating platform.

[0008] Further improvement of the above scheme is that the transfer module comprises a pipe clamping transfer device, a plate clamping transfer device, and a pipetting device, the pipe clamping transfer device is used to complete the operations of clamping and opening, the plate clamping transfer device is used to complete the operation of transporting micro-hole plates, and the pipetting device is used to complete the operation of pipetting.

[0009] Further improvement of the above scheme is that the pipe clamping transfer device comprises an XYZ-axis motion drive assembly arranged on a rack, a pipe clamping assembly support seat, and a pipe clamping assembly rotatably arranged on the pipe clamping assembly support seat, the XYZ-axis motion drive assembly is drivingly connected with the pipe clamping assembly support seat; the pipe clamping assembly comprises a rotating drive assembly rotatably arranged on the support seat, a rotating seat rotatably connected with a power output end of the rotating drive assembly, a pipe clamping drive assembly arranged on the rotating seat, and a pipe clamping jaw slidingly connected with the rotating seat in a horizontal direction, the pipe clamping drive assembly is drivingly connected with the pipe clamping jaw, and an anti-skid pattern is further arranged on the pipe clamping jaw.

[0010] Further improvement of the above scheme is that the plate clamping transfer device comprises a multi-axis plate clamping manipulator assembly, the multi-axis plate clamping manipulator assembly comprises an X-axis sliding rail arranged on a rack, an X-axis drive assembly, a Y-axis sliding support seat slidingly arranged on the X-axis sliding rail in an X-axis direction, a Y-axis drive assembly, and a plate clamping hand support assembly slidingly arranged on the Y-axis sliding support seat in a Y-axis direction, the X-axis drive assembly is drivingly connected with the Y-axis sliding support seat, and the Y-axis drive assembly is drivingly connected with the plate clamping hand support assembly; the plate clamping hand support assembly comprises a plate clamping hand support seat, a plate clamping hand sliding seat slidingly arranged on the plate clamping hand support seat in a Y-axis direction, a plate clamping hand sliding drive assembly arranged on the plate clamping hand support seat, a plate clamping hand rotating seat rotatably arranged on the plate clamping hand sliding seat in a horizontal direction, a plate clamping hand rotating drive assembly arranged on the plate clamping hand sliding seat, two L-shaped clamping jaws oppositely slidingly arranged on the plate clamping hand sliding seat in an X-axis direction, and an L-shaped clamping jaw drive assembly arranged on the plate clamping hand rotating seat, the plate clamping hand sliding drive assembly is drivingly connected with the plate clamping hand sliding seat, the plate clamping hand rotating drive assembly is drivingly connected with the plate clamping hand rotating seat, and the L-shaped clamping jaw drive assembly is drivingly connected with the L-shaped clamping jaw.

[0011] A further improvement to the above solution is that the clamping plate transfer device further includes a rotating plate transfer assembly disposed on the top of the frame. The rotating plate transfer assembly includes a rotating plate transfer slide rail disposed on the frame, a rotating plate transfer sliding seat slidably connected to the rotating plate transfer slide rail, a rotating plate transfer sliding drive assembly disposed on the rotating plate transfer slide rail, a Z-axis sliding arm slidably connected to the rotating plate transfer slide rail along the Z-axis direction, a Z-axis sliding arm drive assembly disposed on the rotating plate transfer sliding seat, a rotating plate support assembly rotatably connected to the bottom of the Z-axis sliding arm, and a clamping plate support assembly disposed on the Z-axis sliding arm. The drive assembly includes a rotating plate transfer sliding drive assembly that drives and connects to the rotating plate transfer sliding seat, a Z-axis sliding arm drive assembly that drives and connects to the Z-axis sliding arm, and a clamping hand rotation drive assembly that drives the rotating plate hand assembly to rotate. The rotating plate hand assembly includes a rotating plate gripper support seat disposed at the bottom of the Z-axis sliding arm, two rotating plate grippers that are slidably disposed relative to each other on the rotating plate gripper support seat along the X-axis, and a rotating plate gripper drive assembly disposed on the rotating plate gripper support seat. The rotating plate gripper drive assembly drives the two rotating plate grippers to move relative to each other along the X-axis.

[0012] A further improvement to the above solution is that the pipetting device includes a pipetting manipulator slide rail disposed on the frame along the X-axis, a pipetting manipulator slide seat slidably disposed on the pipetting manipulator slide rail along the X-axis, a pipetting manipulator support seat disposed on the pipetting manipulator slide seat, a pipette slidably disposed on the pipetting support seat along the Y-axis, and a pipetting manipulator drive assembly disposed on the pipetting manipulator support seat. The pipetting manipulator drive assembly is driven and connected to the pipette, and the pipette is provided with multiple pipetting tips.

[0013] A further improvement to the above scheme is that it also includes a quality analysis component, which includes a camera and a cell 3D imager; the camera is mounted on the rack and is used to capture cell morphology before and after organoid drug administration; the cell 3D imager is mounted on the rack and works with the transfer module to complete cell imaging.

[0014] The beneficial effects of this utility model are as follows: This utility model includes a frame, a workbench mounted on the frame, a transfer module, a chemiluminescence detection mechanism, a low-temperature storage box, a carbon dioxide incubator, and a cell counter. The workbench is used to store consumables and provide a working area for the drug sensitivity detection process. The chemiluminescence detection mechanism is used for drug sensitivity detection. The low-temperature storage box is used to store experimental consumables. The carbon dioxide incubator is used to provide a suitable growth environment for organoids. The cell counter is used to detect the number of cells. The transfer module is used to add drugs stored in the low-temperature storage box to the organoids, and then place the drug-added organoid culture microplate into the carbon dioxide incubator for culture. After the culture is completed, the organoids are sent to the chemiluminescence detection mechanism for drug sensitivity detection.

[0015] This utility model's transfer module integrates a chemiluminescence detection mechanism, a low-temperature storage box, a carbon dioxide incubator, and a cell counter. The transfer module performs operations such as clamping the tube, opening the cap, clamping the plate, and pipetting. The module adds the drug stored in the low-temperature storage box to the organoids, and then places the drug-containing organoid culture microplate into the carbon dioxide incubator for cultivation. After cultivation, the chemiluminescence detection mechanism performs drug sensitivity testing, and the cell counter counts the cells to export the drug sensitivity data. This fully automates the drug sensitivity testing process for organoids without manual intervention, reducing experimental errors. Furthermore, the modules work together seamlessly, eliminating the need to wait for the previous process to complete; idle modules can alternately perform tasks, effectively making it suitable for large-scale operations. This not only improves work efficiency but also replaces manual operation, ensuring stability. Attached image description:

[0016] Figure 1 This is a schematic diagram of the structure of this utility model.

[0017] Figure 2 This is a schematic diagram of the structure of the tube clamping and transferring device of this utility model.

[0018] Figure 3 This is a schematic diagram of the structure of the multi-axis clamping manipulator assembly of this utility model.

[0019] Figure 4 This is a schematic diagram of the structure of the plate transfer assembly of this utility model.

[0020] Figure 5 This is a schematic diagram of the pipetting device of this utility model.

[0021] Figure 6 This is a schematic diagram of the stacking module inside the carbon dioxide incubator of this utility model.

[0022] Figure 7 This is a comparison chart of the working time of the drug sensitivity detection device of this utility model and the working time of manual operation.

[0023] Figure 8 This is a comparison chart showing the stability of the drug sensitivity testing process of this utility model with that of manual experiments.

[0024] Explanation of reference numerals in the attached drawings: Frame 1, Worktable 11, Transfer module 12, Chemiluminescence detection mechanism 13, Chemiluminescence analyzer 131, Low-temperature storage box 14, Carbon dioxide incubator 15, Cell counter 16, Tube clamping and transfer device 2, XYZ axis motion drive assembly 21, Tube clamping assembly support 22, Tube clamping assembly 23, Rotation drive assembly 231, Rotating seat 232, Tube clamping drive assembly 233, Tube clamping claw 234, Anti-slip texture 235, Clamping plate transfer device 3, Multi-axis clamping plate manipulator 31, X-axis slide rail 311, X-axis drive assembly 312, Y-axis sliding support 313, Y-axis drive assembly 314, Clamping plate support assembly 315. Clamping hand support base 3151, clamping hand sliding base 3152, clamping hand sliding drive assembly 3153, clamping hand rotating base 3154, clamping hand rotating drive assembly 3155, L-shaped hand 3156, L-shaped hand drive assembly 3157, pipetting device 4, pipetting robot slide rail 41, pipetting robot sliding base 42, pipetting robot support base 43, pipette 44, pipetting tip 441, pipetting robot drive assembly 45, rotating stack module 51, stack base 511, rotating platform 512, rotating drive assembly 513, shelf 514, quality analysis assembly 6, camera 61, cell 3D imager 62. Detailed implementation method:

[0025] The present invention will be further described below with reference to the accompanying drawings, such as... Figures 1-8 As shown, this utility model is an automated device for cell drug sensitivity testing, including a frame 1, a workbench 11 mounted on the frame 1, a transfer module 12, a chemiluminescence detection mechanism 13, a low-temperature storage box 14, a carbon dioxide incubator 15, and a cell counter 16. The workbench 11 is used to store consumables and provide a working area for the drug sensitivity testing process. The chemiluminescence detection mechanism 13 is used to detect chemiluminescence to determine the drug's properties. The low-temperature storage box 14 is used to store experimental consumables. The carbon dioxide incubator 15 is used to provide a suitable growth environment for organoids. The cell counter 16 is used to detect the number of cells. The drug stored in the low-temperature storage box 14 is added to the organoids through the transfer module 12. Then, the microplate containing the drug-added organoid culture is placed into the carbon dioxide incubator 15 for culture through the transfer module 12. After culture, drug sensitivity testing is performed by the chemiluminescence detection mechanism 13. After the test is completed, the drug sensitivity test data is exported after counting by the cell counter 16.

[0026] The chemiluminescence detection mechanism 13 of this invention includes a chemiluminescence analyzer 131 and a luminescent reagent stored in a low-temperature storage box 14. The luminescent reagent is added to the drug detection microplate through a transfer module 12. The chemiluminescence analyzer 131 is used to detect ATP content and perform result analysis. The chemiluminescence analyzer 131 of this invention is preferably an enzyme-linked immunosorbent assay (ELISA) reader for easy detection.

[0027] In the low-temperature storage box 14 and the carbon dioxide incubator 15 of the present utility model, a rotary stacking module 51 for storing microplates is provided. The rotary stacking module 51 includes a stacking base 511, a rotary platform 512 rotatably arranged on the stacking base 511, a rotary driving component 513 arranged on the stacking base 511, and a storage rack 514 arranged on the rotary platform 512 for storing materials. The rotary driving component 513 is drivingly connected to the rotary platform 512. The rotary stacking module 51 can work in a rotating manner. Multiple storage racks 514 can be provided on the stacking mechanism. The storage rack 514 is divided into areas for storing microplates or culture dishes, with high stacking efficiency and large stacking capacity.

[0028] The transfer system 201 of the present utility model includes a tube clamping transfer device 2, a clamping plate transfer device 3 for clamping and transferring microplates, and a liquid transfer device 4 for sucking and transferring reagent solutions. The tube clamping transfer device 2 is used to complete the operations of clamping and transferring reagent tubes and / or opening caps between the pretreatment module 18 and the automated culture module 19. The clamping plate transfer device 3 is used to complete the operation of clamping microplates between the drug sensitivity detection module 20, the automated culture module 19 and the workbench 17. The liquid transfer device 4 is used to complete the liquid transfer operation between the automated culture module 19, the drug sensitivity detection module 20 and the workbench 17. Compared with the existing organoid workstations, which only simply stack and place processing devices or systems and still require staff to cooperate in operations and handle emergencies of processing equipment or systems, and cannot achieve true automation. The transfer system 201 of the present utility model is divided into a tube clamping transfer device 2, a clamping plate transfer device 3 and a liquid transfer device 4, which can complete a series of manual operations such as transferring microplates, transferring reagent tubes, liquid transfer, and dropping liquid during organoid culture to drug sensitivity detection. It replaces all manual operations that cannot be completed by the equipment in the organoid workstation. With the equipment modules of this workstation, it can completely achieve the full automation of the process from organoid culture to drug sensitivity detection. As Figures 7-8 shown, 20 samples for交接药敏(交接药敏 is not clear in the context and may be a misspelling, assume it's something like "drug sensitivity transfer") are prepared. For each sample, 3 groups of drugs are spread manually and automatically, with 3 replicates in each group. After drug sensitivity detection, the Z values of 40 samples are statistically calculated. If the Z value ≥ 0.5, it is qualified. The automation stability and manual stability of the workstation of the present utility model are calculated. It can be seen that different devices of the workstation of the present utility model cooperate with each other and work with each module in the workstation. It can not only complete a series of work from organoid culture to drug sensitivity detection efficiently and quickly, effectively avoiding the high error rate and instability of manual operations, but also the different devices work independently without affecting each other. A single device failure will not affect the work of other devices, ensuring stability while improving work efficiency. Compared with the inefficient manual operation experiments, it can be further applied to large-scale and high-output batch organoid culture and drug sensitivity detection workplaces.

[0029] The tube clamping and transferring device 2 of this utility model includes an XYZ axis motion drive assembly 21 mounted on a frame 1, a tube clamping assembly support 22, and a tube clamping assembly 23 rotatably mounted on the support 1. The XYZ axis motion drive assembly 21 is driven and connected to the tube clamping assembly support 22. The tube clamping assembly 23 includes a rotation drive assembly 231 rotatably mounted on the support 1, a rotating seat 232 rotatably connected to the power output end of the rotation drive assembly 231, a tube clamping drive assembly 233 mounted on the rotating seat 232, and a component slidably connected to the rotating seat 232 in the horizontal direction. The clamping claw 234 on 32 is driven and connected to the clamping drive assembly 233. The clamping claw 234 is also provided with anti-slip texture 235. The clamping and transferring device 2 completes the clamping and transferring of reagent tubes between various modules. At the same time, the clamping claw 234 is provided with anti-slip texture 235. Compared with ordinary robotic arms, the anti-slip texture 235 can ensure that the clamped reagent tubes will not slip. It can also complete the opening and closing of reagent tubes, which can be better suited to organoid-related workflows.

[0030] The clamping plate transfer device 3 of this utility model includes a multi-axis clamping plate manipulator assembly 31. The multi-axis clamping plate manipulator assembly 31 includes an X-axis slide rail 311 mounted on a frame 1, an X-axis drive assembly 312, a Y-axis sliding support seat 313 slidably mounted on the X-axis slide rail 311 along the X-axis direction, a Y-axis drive assembly 314, and a clamping plate support hand assembly 315 slidably mounted on the Y-axis sliding support seat 313 along the Y-axis direction. The X-axis drive assembly 312 is driven and connected to the Y-axis sliding support seat 313, and the Y-axis drive assembly 314 is driven and connected to the clamping plate support hand assembly 315. The clamping plate support hand assembly 315 includes a clamping plate support hand support seat 3151, a clamping plate support hand sliding seat 3152 slidably mounted on the clamping plate support hand support seat 3151 along the Y-axis direction, and a clamping plate support hand sliding drive assembly 3153 mounted on the clamping plate support hand support seat 3151. The system includes a clamping handle rotating seat 3154, a clamping handle rotating drive assembly 3155, two L-shaped grippers 3156, and an L-shaped gripper driving assembly 3157. The clamping handle rotating drive assembly 3153 drives and connects to the clamping handle sliding seat 3152, the clamping handle rotating drive assembly 3155 drives and connects to the clamping handle rotating seat 3154, and the L-shaped gripper driving assembly 3157 drives and connects to the L-shaped grippers 3156. The L-shaped grippers 3156 can lift the microporous plate or retract the opposite L-shaped grippers 3156 to clamp the microporous plate, thus completing the transportation of microporous plates or plates between various modules.

[0031] The clamping plate transfer device 3 of this utility model further includes a rotating plate transfer assembly 32 disposed on the top of the frame. The rotating plate transfer assembly 32 includes a rotating plate transfer slide rail 321 disposed on the frame, a rotating plate transfer sliding seat 322 slidably connected to the rotating plate transfer slide rail 321, a rotating plate transfer sliding drive assembly 323 disposed on the rotating plate transfer slide rail 321, a Z-axis sliding arm 324 slidably connected to the rotating plate transfer slide rail 321 along the Z-axis direction, and a Z-axis sliding arm 324 disposed on the rotating plate transfer sliding seat 322. Z-axis sliding arm drive assembly 325, a rotating plate support assembly 327 rotatably connected to the bottom of the Z-axis sliding arm 324, and a clamping plate support rotation drive assembly 326 disposed on the Z-axis sliding arm 324; a rotating plate transfer sliding drive assembly 323 drives and connects to a rotating plate transfer sliding seat 322; a Z-axis sliding arm drive assembly 325 drives and connects to the Z-axis sliding arm 324; and a clamping plate support rotation drive assembly 326 drives the rotating plate support assembly 327 to rotate; the rotating plate support assembly 327 includes components disposed on the Z-axis... The sliding arm 324 has a rotating plate gripper support 3271 at its bottom, two rotating plate grippers 3272 that are slidably mounted on the rotating plate gripper support 3271 along the X-axis, and a rotating plate gripper drive assembly 3273 mounted on the rotating plate gripper support 3271. The rotating plate gripper drive assembly 3273 drives the two rotating plate grippers 3272 to move relative to each other along the X-axis. The rotating plate transfer assembly 32 can complete the work of transferring micro-perforated plates on the frame. It can work in conjunction with the multi-axis clamping robot assembly 31 to complete the operation of transporting micro-perforated plates between various modules. Moreover, it can complete the operation of transporting micro-perforated plates that need to be completed when the multi-axis clamping robot assembly 31 is busy. Compared with the traditional single robot arm to complete the work, the rotating plate transfer assembly of this utility model can not only complete the work that the multi-axis clamping robot assembly 31 cannot complete when busy, but also replace the work when the multi-axis clamping robot assembly 31 fails. This improves both work efficiency and the stability of the workstation of this utility model.

[0032] The present invention relates to a pipetting device 4, comprising a pipetting manipulator slide rail 41 disposed on a frame 1 along the X-axis, a pipetting manipulator slide base 42 slidably disposed on the pipetting manipulator slide rail 41 along the X-axis, a pipetting manipulator support base 43 disposed on the pipetting manipulator slide base 42, a pipette 44 slidably disposed on the pipetting support base along the Y-axis, and a pipetting manipulator drive assembly 45 disposed on the pipetting manipulator support base 43. The pipetting manipulator drive assembly 45 drives and connects to the pipette 44, and the pipette 44 is provided with multiple... Each pipette tip 441; the pipetting robot, in conjunction with the pipette 44, can complete the operations of transferring solutions and reagents required in the process from organoid culture to drug sensitivity testing. The pipette 44 is equipped with multiple pipette tips 441, which can complete multiple sets of pipetting operations at the same time, effectively improving work efficiency. It can also be used to pipette different reagents, avoiding cross-infection, without the need to change pipette tips 141. Compared with the traditional manual operation of pipettes, it is better suited for work occasions such as organoid culture and drug sensitivity testing that require frequent pipetting and liquid changing.

[0033] This utility model also includes a quality analysis component 6, which includes a camera 61 and a cell 3D imager 62. The camera 61 is mounted on the frame 1 and is used to capture the cell morphology before and after drug administration to the organoids. The cell 3D imager 62 is mounted on the frame 1 and works with the transfer module 12 to complete cell imaging, thereby determining the state of the organoids after drug administration and performing result analysis.

[0034] Working principle:

[0035] This utility model's transfer module 12 integrates a chemiluminescence detection mechanism 13, a low-temperature storage box 14, a carbon dioxide incubator 15, and a cell counter 16. The transfer module 12 performs operations such as clamping tubes, opening caps, clamping plates, and pipetting. Organoids are added to drugs stored in the low-temperature storage box 14 via the transfer module 12. Then, the drug-containing organoid culture microplate is placed into the carbon dioxide incubator 15 for culture. After culture, drug sensitivity testing is performed via the chemiluminescence detection mechanism 13. After testing, the drug sensitivity data is exported via the cell counter 16. This fully automates the organoid drug sensitivity testing process without manual intervention, reducing experimental errors. Furthermore, the modules work together seamlessly, eliminating the need to wait for the previous process to complete. Idle modules can alternately perform tasks, effectively adapting to large-scale operations. This not only improves work efficiency but also replaces manual operation, ensuring stability.

[0036] Of course, the above description is only a preferred embodiment of the present utility model. Therefore, all equivalent changes or modifications made to the structure, features and principles described in the claims of the present utility model patent application are included in the scope of the present utility model patent application.

Claims

1. A cell drug sensitivity test automation apparatus, characterized by: The application relates to a kind of organoid drug sensitivity detection device, including rack (1), workbench (11) arranged on rack (1), transfer module (12), chemiluminescence detection mechanism (13), low-temperature storage box (14), carbon dioxide incubator (15), cell counter (16), the workbench (11) is used to store consumables and provide work area for drug sensitivity detection process, the chemiluminescence detection mechanism (13) is used for drug sensitivity detection, the low-temperature storage box (14) is used to store experimental consumables, the carbon dioxide incubator (15) is used to provide suitable growth environment for organoid, the cell counter (16) is used to detect cell quantity;The transfer module (12) is used to add drug to organoid stored in low-temperature storage box (14), then the organoid with drug is cultured in carbon dioxide incubator (15), and after culturing, the organoid is sent to chemiluminescence detection mechanism (13) for drug sensitivity detection, the transfer module (12) includes pipe clamp transfer device (2), clamping plate transfer device (3) and pipetting device (4), the pipe clamp transfer device (2) is used to complete clamping, opening cover operation, the clamping plate transfer device (3) is used to complete the operation of conveying micro-hole plate, and the pipetting device (4) is used to complete the operation of pipetting.

2. The automatic device for cell drug sensitivity test according to claim 1, characterized in that: The chemiluminescence detection mechanism (13) includes chemiluminescence instrument (131), chemiluminescence reagent stored in the low-temperature storage box (14), the chemiluminescence reagent is added into drug detection micro-hole plate by transfer module (12), and the chemiluminescence instrument (131) is used to detect ATP content.

3. The automated cell drug sensitivity detection device according to claim 2, wherein: The chemiluminescence instrument (131) is an enzyme label instrument.

4. The automated cell drug sensitivity detection device according to claim 1, wherein: The low-temperature storage box (14) and carbon dioxide incubator (15) are provided with rotatable stack modules (51) capable of storing micro-hole plates, the rotatable stack module (51) includes stack base (511), rotatable platform (512) rotatably arranged on the stack base (511), rotatable driving assembly (513) arranged on the stack base (511), and material storage rack (514) arranged on the rotatable platform (512) and used to store materials, and the rotatable driving assembly (513) is drivingly connected with the rotatable platform (512).

5. The automated cell drug sensitivity detection device according to claim 1, wherein: The pipe clamp transfer device (2) includes XYZ-axis motion driving assembly (21) arranged on the rack (1), pipe clamp component support seat (22), pipe clamp component (23) rotatably arranged on the pipe clamp component support seat (22), the XYZ-axis motion driving assembly (21) is drivingly connected with the pipe clamp component support seat (22), the pipe clamp component (23) includes rotatable driving assembly (231) rotatably arranged on the pipe clamp component support seat (22), rotatable seat (232) rotatably connected with the power output end of the rotatable driving assembly (231), pipe clamp driving assembly (233) arranged on the rotatable seat (232), and pipe clamp claw (234) slidingly connected with the rotatable seat (232) in horizontal direction, the pipe clamp driving assembly (233) is drivingly connected with the pipe clamp claw (234), and the pipe clamp claw (234) is further provided with anti-skid lines (235).

6. The automated cell drug sensitivity detection device according to claim 1, wherein: The clamp plate conveying device (3) comprises a multi-axis clamp plate manipulator assembly (31), the multi-axis clamp plate manipulator assembly (31) comprises an X-axis sliding rail (311) arranged on the rack (1), an X-axis driving assembly (312), a Y-axis sliding support seat (313) arranged on the X-axis sliding rail (311) and sliding in the X-axis direction, a Y-axis driving assembly (314), a clamp plate supporting hand assembly (315) arranged on the Y-axis sliding support seat (313) and sliding in the Y-axis direction, the X-axis driving assembly (312) is drivingly connected with the Y-axis sliding support seat (313), and the Y-axis driving assembly (314) is drivingly connected with the clamp plate supporting hand assembly (315); the clamp plate supporting hand assembly (315) comprises a clamp plate supporting hand support seat (3151), a clamp plate supporting hand sliding seat (3152) arranged on the clamp plate supporting hand support seat (3151) and sliding in the Y-axis direction, a clamp plate supporting hand sliding driving assembly (3153) arranged on the clamp plate supporting hand support seat (3151), a clamp plate supporting hand rotating seat (3154) arranged on the clamp plate supporting hand sliding seat (3152) and rotating in the horizontal direction, a clamp plate supporting hand rotating driving assembly (3155) arranged on the clamp plate supporting hand sliding seat (3152), two L-shaped clamping jaws (3156) arranged on the clamp plate supporting hand sliding seat (3152) and sliding in the X-axis direction, and an L-shaped clamping jaw driving assembly (3157) arranged on the clamp plate supporting hand rotating seat (3154); the clamp plate supporting hand sliding driving assembly (3153) is drivingly connected with the clamp plate supporting hand sliding seat (3152), the clamp plate supporting hand rotating driving assembly (3155) is drivingly connected with the clamp plate supporting hand rotating seat (3154), and the L-shaped clamping jaw driving assembly (3157) is drivingly connected with the L-shaped clamping jaw (3156).

7. The automated cell drug sensitivity detection device according to claim 1, wherein: The clamp plate transfer device (3) further comprises a rotating plate transfer assembly (32) arranged on the top of the rack (1), the rotating plate transfer assembly (32) comprises a rotating plate transfer sliding rail (321) arranged on the rack (1), a rotating plate transfer sliding seat (322) slidingly connected to the rotating plate transfer sliding rail (321), a rotating plate transfer sliding drive assembly (323) arranged on the rotating plate transfer sliding rail (321), a Z-axis sliding arm (324) slidingly connected to the rotating plate transfer sliding rail (321) along the Z-axis direction, a Z-axis sliding arm drive assembly (325) arranged on the rotating plate transfer sliding seat (322), a rotating plate supporting hand assembly (327) rotationally connected to the bottom of the Z-axis sliding arm (324), and a clamp plate supporting hand rotating drive assembly (326) arranged on the Z-axis sliding arm (324), the rotating plate transfer sliding drive assembly (323) is drivingly connected to the rotating plate transfer sliding seat (322), the Z-axis sliding arm drive assembly (325) is drivingly connected to the Z-axis sliding arm (324), and the clamp plate supporting hand rotating drive assembly (326) drives the rotating plate supporting hand assembly (327) to rotate; the rotating plate supporting hand assembly (327) comprises a rotating plate clamp jaw support seat (3271) arranged on the bottom of the Z-axis sliding arm (324), two rotating plate clamp jaws (3272) slidingly arranged on the rotating plate clamp jaw support seat (3271) in the X-axis direction, and a rotating plate clamp jaw drive assembly (3273) arranged on the rotating plate clamp jaw support seat (3271), the rotating plate clamp jaw drive assembly (3273) drives the two rotating plate clamp jaws (3272) to move in the X-axis direction.

8. The automated cell drug sensitivity detection device according to claim 1, wherein: The pipetting device (4) comprises a pipetting manipulator sliding rail (41) arranged on the rack (1) in the X-axis direction, a pipetting manipulator sliding seat (42) slidingly arranged on the pipetting manipulator sliding rail (41) in the X-axis direction, a pipetting manipulator support seat (43) arranged on the pipetting manipulator sliding seat (42), a pipette (44) slidingly arranged on the pipetting support seat in the Y-axis direction, and a pipetting manipulator drive assembly (45) arranged on the pipetting manipulator support seat (43), the pipetting manipulator drive assembly (45) is drivingly connected to the pipette (44), and the pipette (44) is provided with a plurality of pipetting heads (441).

9. The automated cell drug sensitivity detection device according to claim 1, wherein: Further comprising a quality analysis assembly (6), the quality analysis assembly (6) comprises a camera and a cell 3D imager (62), the camera is arranged on the rack (1) and is used for shooting the cell morphology before and after the organoid is added with drugs, and the cell 3D imager (62) is arranged on the rack (1) and cooperates with the transfer module (12) to complete cell imaging.

Images