Portable virus detector

Abstract

The utility model discloses a portable virus detector, which belongs to the technical field of medical detection equipment and comprises a box body, and an inner cavity of the box body is independently provided with a collection cavity and a detection cavity; the collecting assembly is arranged in the collecting cavity and is used for collecting a sample; the centrifugal motor is mounted in the detection cavity; the micro-fluidic chip is arranged in the detection cavity and is connected with the driving end of the centrifugal motor; the centrifugal motor is used for driving the micro-fluidic chip to rotate at a high speed; the display mechanism is arranged on the outer side of the box body, is in wireless connection with the micro-fluidic chip and is used for setting detection parameters, starting a detection process and checking a detection result. The device is light and small in design, integrates a sample collection module, a sample treatment module, a nucleic acid amplification module and a detection module in one device, reduces external connection and accessories, and is more compact and convenient to carry.

Description

TECHNICAL FIELD

[0001] The utility model belongs to medical detection equipment technical field, specifically, relate to a portable virus detection appearance. BACKGROUND

[0002] At present, the virus detection appearance on market is mostly compared huge, carries not convenient. In the virus detection, mostly is on -the -spot sampling, carries out the removal to the detection medium solution, observes the detection medium solution to get the detection result, and the detection is complex, and the detection result is slow.

[0003] Therefore, the portable virus detection appearance of quick detection is urgently needed. UTILITY MODEL CONTENT

[0004] In order to realize the above purpose, the utility model adopts the following technical scheme:

[0005] A portable virus detection appearance, it includes:

[0006] Box, the inner chamber of box is independently provided with collection cavity and detection cavity;

[0007] Collection assembly, the collection assembly is arranged in the collection cavity, and is used for collecting sample;

[0008] Centrifugal motor, the centrifugal motor is installed in the detection cavity;

[0009] Microfluidic chip, the microfluidic chip is arranged in the detection cavity, and is connected with the drive end of centrifugal motor;The centrifugal motor is used to drive microfluidic chip high -speed rotation;

[0010] Display mechanism, the display mechanism is arranged on the outside of box, and is wirelessly connected with the microfluidic chip, and is used for setting detection parameter, starting detection flow and viewing detection result.

[0011] Further, the collection assembly includes virus collection pipe and virus collection swab;

[0012] The collection cavity is provided with 2, and two collection cavities are oppositely arranged on the two sides of detection cavity;The virus collection pipe and the virus collection swab are arranged in two collection cavities respectively.

[0013] Further, the microfluidic chip is circular structure, and the center of the microfluidic chip is provided with positioning hole, and the positioning hole is drivenly connected with the output end of centrifugal motor;

[0014] The microfluidic chip is evenly distributed with a plurality of detection modules along the circumference, and the detection module is connected with the display mechanism.

[0015] Furthermore, the detection module includes an optical detection unit, and a nucleic acid extraction unit and a nucleic acid detection unit arranged from the center outwards, wherein the nucleic acid extraction unit and the nucleic acid detection unit are connected.

[0016] The optical detection unit is connected to the display mechanism, and the optical detection unit is configured correspondingly to the nucleic acid detection unit for monitoring the fluorescence signal of the nucleic acid detection unit.

[0017] Furthermore, the nucleic acid extraction unit includes a first washing tank, a lysis tank, and an adsorption tank arranged sequentially from the center outwards; the first washing tank contains a washing solution, the lysis tank contains a lysis solution, and the adsorption tank contains a magnetic bead solution.

[0018] The nucleic acid extraction unit further includes a first isolation tank, a second washing tank, a second isolation tank, and an elution tank; the first isolation tank, the second washing tank, the second isolation tank, and the elution tank are connected in sequence; wherein, the adsorption tank is connected to the first isolation tank, and the adsorption tank, the first isolation tank, the second washing tank, the second isolation tank, and the elution tank are distributed along the circumferential direction of the microfluidic chip;

[0019] The elution tank is connected to the nucleic acid detection unit.

[0020] Furthermore, each of the first washing tank, the pyrolysis tank, the adsorption tank, the first isolation tank, the second washing tank, the second isolation tank, and the elution tank is provided with a sample inlet.

[0021] Furthermore, the nucleic acid detection unit includes a waste liquid tank, a gear-shaped distribution tank, and several reaction tanks distributed circumferentially; wherein, the distribution tank is connected to the reaction tank through a pipe, and the reaction tank is located at the tip of the gear of the distribution tank; the first end of the distribution tank is connected to the elution tank, and the second end of the distribution tank is connected to the waste liquid tank;

[0022] The waste liquid tank is equipped with an exhaust port, the reaction tank is equipped with isothermal amplification reagents, and the pipeline is equipped with a capillary valve.

[0023] The optical detection unit is configured correspondingly to the reaction cell, and the capillary valve is connected to the display mechanism.

[0024] The beneficial effects of this utility model are:

[0025] 1. Portability: This utility model is designed to be lightweight and compact, integrating sample collection, processing, nucleic acid amplification and detection modules into one device, reducing external connections and accessories, making the device more compact and easy to carry;

[0026] 2、Automation, the utility model discloses utilize micro -fluidic chip to carry out nucleic acid extraction and purification, avoid manual operation, reduce the pollution risk and the operating error. The fluorescence signal of amplification product is automatically monitored through optical detection unit, and real-time record data, and automatically analyze and handle, generate quantitative or qualitative detection result. Develop intuitive and easy-to-use user interface, through touch screen or mobile device, user can easily set detection parameter, start detection flow and view result, reduce the operation complexity;

[0027] 3、Fast detection, the utility model discloses through adopting isothermal amplification technique, carries out nucleic acid amplification under constant temperature quickly, shortens detection time;Optimize micro -fluidic chip design, improve the speed of sample processing and nucleic acid extraction, ensure that the whole detection process is carried out efficiently. BRIEF DESCRIPTION OF DRAWINGS

[0028] Fig. 1 It is the structural diagram of portable virus detection instrument of the utility model;

[0029] Fig. 2 It is the structural diagram of micro -fluidic chip of the utility model;

[0030] In the drawing: 1, box body;2, collection cavity;3, micro -fluidic chip;301, sample inlet hole;302, positioning hole;303, exhaust hole;31, first cleaning pool;32, lysis pool;33, adsorption pool;34, first isolation pool;35, second cleaning pool;36, second isolation pool;37, elution pool;38, waste liquid pool;39, reaction pool;4, display mechanism. DETAILED DESCRIPTION

[0031] It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict. The utility model will be described in detail below with reference to the drawings and in combination with embodiments.

[0032] Embodiment 1

[0033] Reference Figs. 1-2 A kind of portable virus detection instrument, comprising:

[0034] Box body 1, the inner cavity of box body 1 is independently provided with collection cavity 2 and detection cavity;

[0035] Collection assembly, collection assembly is set in collection cavity 2, for collecting sample;

[0036] Centrifugal motor, centrifugal motor is installed in detection cavity;

[0037] Micro -fluidic chip 3, micro -fluidic chip 3 is set in detection cavity, and is connected with the drive end of centrifugal motor;Centrifugal motor is used to drive micro -fluidic chip 3 high-speed rotation;

[0038] The display mechanism 4 is arranged outside the box body 1 and is wirelessly connected with the microfluidic chip 3, and is used for setting detection parameters, starting a detection process and viewing detection results.

[0039] In the embodiment, the display mechanism 4 is a touch screen.

[0040] In other embodiments, the display mechanism 4 is a mobile device, preferably a mobile phone.

[0041] Preferably, the collection assembly includes a virus collection tube and a virus collection swab.

[0042] The collection cavities 2 are arranged in pairs on two sides of the detection cavity; the virus collection tube and the virus collection swab are arranged in the two collection cavities 2, respectively.

[0043] In the embodiment, the virus collection tube is made of polypropylene, the bottle opening is a spiral opening, the tube body is transparent, and a red preservative liquid is arranged inside.

[0044] The virus collection swab is composed of a flocked head and an ABS plastic rod, and the breaking point is located about 3 cm from the top end of the swab, which is easy to break.

[0045] Preferably, the microfluidic chip 3 is in a circular structure, and a positioning hole 302 is arranged at the center of the microfluidic chip 3 and is drivingly connected with the output end of the centrifugal motor.

[0046] The microfluidic chip 3 is uniformly distributed with a plurality of detection modules along the circumferential direction, and the detection modules are connected with the display mechanism 4.

[0047] Preferably, the detection module includes an optical detection unit, and nucleic acid extraction and detection units arranged outward from the center; the nucleic acid extraction unit is in communication with the nucleic acid detection unit.

[0048] The optical detection unit is connected with the display mechanism 4, and the optical detection unit and the nucleic acid detection unit are arranged correspondingly, and are used for monitoring the fluorescence signal of the nucleic acid detection unit.

[0049] Preferably, the nucleic acid extraction unit includes a first washing pool 31, a lysis pool 32 and an adsorption pool 33 arranged in sequence and in communication from the center outward; the first washing pool 31 is arranged with a washing liquid, the lysis pool 32 is arranged with a lysis liquid, and the adsorption pool 33 is arranged with a magnetic bead solution.

[0050] The nucleic acid extraction unit further includes a first isolation pool 34, a second washing pool 35, a second isolation pool 36 and an elution pool 37; the first isolation pool 34, the second washing pool 35, the second isolation pool 36 and the elution pool 37 are sequentially connected; wherein the adsorption pool 33 is in communication with the first isolation pool 34, and the adsorption pool 33, the first isolation pool 34, the second washing pool 35, the second isolation pool 36 and the elution pool 37 are distributed along the circumferential direction of the microfluidic chip 3.

[0051] The elution pool 37 is in communication with the nucleic acid detection unit.

[0052] Preferably, the first washing pool 31, the lysis pool 32, the adsorption pool 33, the first isolation pool 34, the second washing pool 35, the second isolation pool 36 and the elution pool 37 are each provided with a sample injection hole 301.

[0053] In this embodiment, the first washing pool 31, the lysis pool 32, the adsorption pool 33, the first isolation pool 34, the second washing pool 35, the second isolation pool 36 and the elution pool 37 are in communication through a first pipeline, and the first pipeline is provided with a first capillary valve; wherein the radius of the first pipeline is greater than the radius of the magnetic beads; the first capillary valve is connected with a display mechanism.

[0054] In this embodiment, the sample injection hole 301 is matched with the size of the tip of a pipette in biological experiments.

[0055] Preferably, the nucleic acid detection unit comprises a waste pool 38, a gear-type distribution pool and a plurality of reaction pools 39 distributed along the circumference; wherein the distribution pool is in communication with the reaction pools 39 through a pipeline, and the reaction pools 39 are located at the gear tips of the distribution pool; the first end of the distribution pool is in communication with the elution pool 37, and the second end of the distribution pool is in communication with the waste pool 38.

[0056] The waste pool 38 is provided with an exhaust hole 303, the reaction pools are provided with isothermal amplification reagents, and the pipeline is provided with a capillary valve;

[0057] The optical detection unit is correspondingly provided with the reaction pools 39, and the capillary valve is connected with a display mechanism.

[0058] In this embodiment, the isothermal amplification reagents are composed of three key enzymes, i.e. recombinase Uvs X, single-strand binding protein Gp32 and strand displacement DNA polymerase, an energy supply system, i.e. adenosine triphosphate, phosphocreatine and creatine kinase, a stable reaction system, i.e. Tris, potassium acetate, high molecular weight polyethylene glycol and dithiothreitol, deoxyribonucleotide triphosphate (dNTP) and magnesium acetate.

[0059] In this embodiment, the microfluidic chip 3 is further provided with a cover sheet.

[0060] Embodiment 2

[0061] This embodiment is the experimental process of the microfluidic chip of embodiment 1.

[0062] After the microfluidic chip is installed with the cover sheet and the corresponding solution is injected, the sample solution is injected into the lysis pool, the instrument is placed in the corresponding installation position, and the instrument door is closed.

[0063] S1, lysis: the microfluidic chip reciprocating rotates at a speed of 120 rpm, so that the sample is mixed in the lysis pool, and nucleic acid in the sample is released in the lysis solution;

[0064] S2, transfer: the microfluidic chip rotates clockwise at a speed of 1500 rpm, the first capillary valve between the lysis pool and the adsorption pool is opened, and the lysis solution and the sample enter the adsorption pool and mix with the magnetic beads;

[0065] S3, washing: the microfluidic chip rotates clockwise at a speed of 2500 rpm, the first capillary valve between the first washing pool and the lysis pool is opened, and the washing solution enters the lysis pool, and the residual sample in the lysis pool is added to the adsorption pool;

[0066] S4, adsorption: the microfluidic chip reciprocating rotates at a speed of 120 rpm, so that the nucleic acid and the magnetic beads are mixed in the pool, and the magnetic bead-nucleic acid complex is formed;

[0067] S5, washing: under the action of the magnetic field, the magnetic beads pass through the first isolation pool and enter the first washing pool, and the microfluidic chip reciprocating rotates at a speed of 100 rpm, so that the protein and other impurities attached to the magnetic bead-nucleic acid complex are removed;

[0068] S6, elution: under the action of the magnetic field, the microfluidic chip reciprocating rotates at a speed of 180 rpm, so that the nucleic acid is separated from the magnetic beads, and under the action of the magnetic field, the magnetic beads move to the second isolation pool to prevent the magnetic beads from entering the reaction pool;

[0069] S7, distribution: the microfluidic chip rotates counterclockwise at a speed of 3000 rpm, the eluted nucleic acid sample follows the solution into the distribution cavity, and the excess solution enters the waste pool, at this time the generated gas is also discharged from the waste pool, and under the action of centrifugal force, the solution can achieve uniform distribution;

[0070] S8, into the reaction pool: the microfluidic chip rotates counterclockwise at a speed of 4500 rpm, and the solution in the distribution pool enters the reaction pool;

[0071] S9, mixing: the microfluidic chip reciprocating rotates at a speed of 100 rpm, so that the nucleic acid in the reaction pool and the amplification reagent are fully mixed, and the constant temperature amplification starts.

[0072] In this embodiment, the basic principle of constant temperature amplification is:

[0073] ①Under the condition of adenosine triphosphate energy supply, the recombinase Uvs X combines with the primer to form an enzyme-primer complex with the assistance of the recombinase loading factor T4 Uvs Y;

[0074] ②The complex finds the gene homologous sequence in the reaction system, and forms a D loop structure at the invasion homologous sequence;

[0075] ③D loop structure on one side is double-stranded, primer hybridization with the template strand, start strand displacement reaction, the other side of the single-stranded by binding to single-stranded binding protein Gp32 into a stable state;

[0076] ④Uvs X dissociation from enzyme-primer complex, DNA polymerase recognition primer free 3'-OH end, adding dNTPs start amplification reaction;

[0077] ⑤ template parent strand separation, DNA daughter strand synthesis;

[0078] ⑥ two DNA daughter strand synthesis, the process continues to cycle.

[0079] In order to be used for RNA template detection, the embodiment adds Murine Leukemia Virus (MuLV) on the basis of the basic amplification reagent, and the MuLV first transcribes the template RNA under the guidance of the RPA primer, so that the RNA is converted into cDNA, and then the reaction starts exponential amplification with the cDNA as the template.

[0080] Embodiment 3

[0081] In order to better detect the sample, the embodiment is further provided on the basis of the embodiment 2.

[0082] On the basis of RPA, exonuclease III and exo fluorescent probe are added, the exo probe is a special oligonucleotide single-stranded DNA homologous to the RPA amplification product, the 3'-OH end is closed, and the DNA polymerase amplification is prevented. The exonuclease III specifically cuts the fluorescent probe (THF site), the fluorescent group and the quencher group are separated, the optical detection unit receives the fluorescent signal, and the change of the fluorescent value is monitored in real time.

[0083] The above only describes preferred embodiments of the present application and is not used to limit the present application. For those skilled in the art, the present application can be variously changed and modified. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application is included in the protection scope of the present application.

Claims

1. A portable virus detector, characterized by comprising: The utility model relates to a portable nucleic acid detection device, including: Box, the inner chamber of box is independently provided with collection cavity and detection cavity; Collection component, the collection component is arranged in the collection cavity, is used for collecting sample; Centrifugal motor, the centrifugal motor is installed in the detection cavity; Microfluidic chip, the microfluidic chip is arranged in the detection cavity, and is connected with the drive end of centrifugal motor;The centrifugal motor is used to drive microfluidic chip high -speed rotation; Display mechanism, the display mechanism is arranged on the outside of box, and is wirelessly connected with microfluidic chip, is used for setting detection parameter, starting detection flow and viewing detection result.

2. The portable virus detector according to claim 1, wherein The collection component includes virus collection pipe and virus collection swab; The collection cavity is provided with 2, 2 the collection cavity is oppositely arranged on the both sides of detection cavity;The virus collection pipe and the virus collection swab are arranged in 2 collection cavities respectively.

3. The portable virus detector according to claim 1, wherein The microfluidic chip is circular structure, the center of the microfluidic chip is provided with positioning hole, and the positioning hole is drivenly connected with the output end of centrifugal motor; The microfluidic chip is uniformly distributed with a plurality of detection modules along the circumference, and the detection modules are connected with the display mechanism.

4. The portable virus detector according to claim 3, wherein The detection module includes an optical detection unit, an nucleic acid extraction unit and an nucleic acid detection unit arranged outward from the center, and the nucleic acid extraction unit is communicated with the nucleic acid detection unit; The optical detection unit is connected with the display mechanism, and the optical detection unit is correspondingly arranged with the nucleic acid detection unit, for monitoring the fluorescence signal of the nucleic acid detection unit.

5. The portable virus detector according to claim 4, wherein The nucleic acid extraction unit includes a first washing pool, a lysis pool and an adsorption pool arranged in sequence and communicated from the center outward;The first washing pool is provided with a washing liquid, the lysis pool is provided with a lysis liquid, and the adsorption pool is provided with a magnetic bead solution; The nucleic acid extraction unit further includes a first isolation pool, a second washing pool, a second isolation pool and an elution pool;The first isolation pool, the second washing pool, the second isolation pool and the elution pool are sequentially connected;Among them, the adsorption pool is communicated with the first isolation pool, and the adsorption pool, the first isolation pool, the second washing pool, the second isolation pool and the elution pool are distributed along the circumferential direction of the microfluidic chip; The elution pool is communicated with the nucleic acid detection unit.

6. The portable virus detector according to claim 5, wherein The first washing pool, the lysis pool, the adsorption pool, the first isolation pool, the second washing pool, the second isolation pool and the elution pool are all provided with a sample inlet hole.

7. The portable virus detector according to claim 5, wherein The nucleic acid detection unit includes a waste liquid pool, a gear type distribution pool and a plurality of reaction pools distributed along the circumference;Among them, the distribution pool is communicated with the reaction pool through a pipeline, and the reaction pool is located at the gear tip of the distribution pool;The first end of the distribution pool is communicated with the elution pool, and the second end of the distribution pool is communicated with the waste liquid pool; The waste liquid pool is provided with an exhaust hole, the reaction pool is provided with an isothermal amplification reagent, and the pipeline is provided with a capillary valve; The optical detection unit is correspondingly arranged with the reaction pool, and the capillary valve is connected with the display mechanism.

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