Integrated experimental device suitable for bacteriostasis verification of photosensitive antibacterial material

Abstract

The utility model discloses an integral experimental device suitable for the bacteriostasis verification of a photosensitive antibacterial material, which comprises an experimental bottle, a separator, an upper cover and a smearing piece, and the bottleneck of the experimental bottle vertically extends upwards; the separator is arranged in the bottle neck and divides a channel of the bottle neck into an upper cavity and a lower cavity which are not communicated with each other; the upper cover detachably covers the top of the bottle neck; and the smearing piece is used for uniformly smearing a photosensitive material and microorganisms on the solid culture medium in the upper cavity. The integral experimental device suitable for the bacteriostasis verification of the photosensitive antibacterial material can effectively solve the problems that the photosensitive material needs to be irradiated by light, and illumination, moisture and nutrition are maintained in the microbial culture process.

Description

TECHNICAL FIELD

[0001] The utility model relates to bacteriostatic verification experiment device, concretely relates to a kind of bacteriostatic verification integrated experiment device suitable for photosensitive antibacterial material. BACKGROUND

[0002] With the blind use of antibiotics, the problem of clinical drug-resistant bacteria is increasingly serious, and new antibacterial strategy research is imminent. Photosensitive antibacterial material becomes the forefront of new antibacterial material research and development. The research and development of full-spectrum (ultraviolet, visible, infrared) response photocatalytic materials are in full swing, but due to the particularity of detection conditions (it is difficult to simultaneously consider the light conditions and the suitable growth conditions of microorganisms), the conventional clinical detection method is not applicable.

[0003] Bacteriostatic test is a test for determining the in vitro bacteriostatic effect of antibacterial drugs. The current detection methods mainly include two categories: liquid and solid. The specific methods are as follows: test tube dilution method, the antibiotic content in the culture medium is diluted by several geometric orders of magnitude and inoculated with an appropriate amount of bacteria. After culture, the minimum antibiotic concentration that can cause bacteriostatic effect is called minimum inhibitory concentration (MIC); OD method (optical density method), the concentration of bacterial solution is proportional to the optical density (usually detected at a specific wavelength of 600 nm) within a certain range, and the microbial concentration can be estimated by OD value; plate coating method, a certain dilution sample is coated on a solid plate, and the number of bacteria on the plate is counted after culture; paper disc diffusion method, a paper disc containing a certain concentration of sample or antibiotic is placed in a solid plate coated with bacteria, and different size diameter bacteriostatic rings are formed after culture. It is the most commonly used drug sensitivity test method in clinical microbiology laboratory.

[0004] Photosensitive antibacterial material generally needs specific spectrum or full spectrum to catalyze and play a bacteriostatic effect. Liquid method is not suitable due to the limitation of light intensity and depth. In addition, the closed environment with cover is also not suitable. Even if the transparent cover hinders the effect of full-spectrum material. Therefore, the detection method and the use of equipment of solid exposure culture need to be further improved. UTILITY MODEL CONTENT

[0005] In view of the deficiencies of the prior art, the utility model solves the technical problem of providing a bacteriostatic verification integrated experiment device suitable for photosensitive antibacterial material, which can effectively solve the problem of light irradiation, moisture and nutrient retention in the process of microbial culture of photosensitive material.

[0006] In order to achieve the above purpose, the utility model is realized by the following technical scheme: a bacteriostatic verification integrated experiment device suitable for photosensitive antibacterial material, comprising:

[0007] Experiment bottle, the neck of the experiment bottle extends vertically upward;

[0008] A partition piece is detachably arranged in the bottle neck to divide the channel of the bottle neck into an upper cavity and a lower cavity which are not communicated;

[0009] An upper cover is detachably arranged on the top of the bottle neck; and

[0010] A smearing piece is arranged for uniformly smearing photosensitive material and microorganisms on the solid culture medium in the upper cavity.

[0011] Further, a side wall of the bottle neck is provided with a bushing hole communicated with the inner cavity, the partition piece is a plug, and the end of the plug is inserted into the bottle neck through the bushing hole outside the bottle neck to divide the channel of the bottle neck into the upper cavity and the lower cavity which are not communicated.

[0012] Further, the partition piece comprises a rotating shaft and a blocking piece, the rotating shaft is transversely arranged in the bottle neck and can rotate, and the blocking piece is fixedly sleeved on the rotating shaft, the shape of the blocking piece is matched with the cross-sectional dimension of the inner cavity of the bottle neck, and the blocking piece can block the channel of the bottle neck when the blocking piece is rotated to a horizontal state.

[0013] Further, the end of the rotating shaft extends to outside of the bottle neck.

[0014] Further, the smearing piece comprises a handle and a smearing part connected to the handle.

[0015] Further, the smearing part is triangular, and one end of the smearing part is connected to the handle.

[0016] Further, the width of the smearing part is 1-2 cm.

[0017] Further, a scale is arranged on the outer wall of the experimental bottle along the height thereof.

[0018] Further, the outer dimension of the experimental bottle is a conical body with a large bottom and a small top.

[0019] The experimental bottle has the advantages that:

[0020] The experimental bottle is applicable to the photosensitive antibacterial material bacteriostasis verification integral type experimental device, in use, the experimental bottle is sterilized first, then the partition piece is drawn out, the partition piece is restored after the sterile distilled water is poured into the experimental bottle, then the LB solid culture medium (or other type culture medium) is prepared, after being sterilized at 121 DEG C for 15 min, the culture medium is cooled to about 60 DEG C, the antibiotic (A) or photosensitive material X is added to the culture medium, and the culture medium is uniformly mixed.

[0021] The application is suitable for the whole type experimental device for the antibacterial verification of the photosensitive antibacterial material, and the lower part of the tube body is communicated with the experimental bottle, so that the moisture and nutrients can be continuously supplemented during the culture process, and the light irradiation effect is not affected due to the removal of the upper cover, so that the problems of the light irradiation, moisture and nutrient maintenance during the culture process of the photosensitive material and the microorganism culture process can be effectively solved. BRIEF DESCRIPTION OF DRAWINGS

[0022] In order to more clearly illustrate the specific embodiments of the present application, the drawings used in the specific embodiments will be briefly introduced. In all the drawings, the elements or parts are not necessarily drawn according to the actual scale.

[0023] Figure 1 A schematic view of the whole type experimental device for the antibacterial verification of the photosensitive antibacterial material is provided for the first embodiment of the present application;

[0024] Figure 2 As shown in Figure 1 As shown in the schematic view of the whole type experimental device for the antibacterial verification of the photosensitive antibacterial material in a disassembled state;

[0025] Figure 3 As shown in Figure 1 As shown in the schematic view of the smearing part in the whole type experimental device for the antibacterial verification of the photosensitive antibacterial material;

[0026] Figure 4 As shown in Figure 1 As shown in the schematic view of the separating part in the whole type experimental device for the antibacterial verification of the photosensitive antibacterial material;

[0027] Figure 5 As shown in the schematic view of the separating part of the whole type experimental device for the antibacterial verification of the photosensitive antibacterial material provided for the second embodiment of the present application;

[0028] Reference signs:

[0029] 100, experimental bottle; 110, bottle neck; 200, separating part; 210, plug; 220, rotating shaft; 230, blocking piece; 300, upper cover; 400, smearing part; 410, handle; 420, smearing part. DETAILED DESCRIPTION

[0030] The embodiments of the technical scheme of the present application will be described in detail below with reference to the drawings. The following embodiments are only used to more clearly illustrate the technical scheme of the present application, and therefore only serve as examples, and cannot limit the protection scope of the present application.

[0031] First embodiment:

[0032] Please refer to Figures 1 to 4The utility model provides a kind of whole experimental device suitable for photosensitive antibacterial material bacteriostatic verification, including experimental bottle 100, separator 200, upper cover 300 and smearing piece 400.

[0033] Specifically, the neck 110 of the experimental bottle 100 extends vertically upward. The outer wall of the experimental bottle 100 is provided with a scale along its height. In specific implementation, the experimental bottle 100 can be selected as a commonly used conical triangular experimental bottle with a large bottom and a small top in the prior art. The separator 200 is arranged in the neck 110, dividing the passage of the neck 110 into an upper cavity and a lower cavity that are not connected. The upper cover 300 is detachably arranged on the top of the neck 110. The smearing piece 400 is used to uniformly smear the photosensitive material and microorganisms on the solid culture medium in the upper cavity.

[0034] In use, the experimental bottle 100 is first sterilized, then the separator 200 is opened, and after pouring sterile distilled water into the experimental bottle 100, the separator 200 is restored. Then, the LB solid culture medium (or other types of culture medium) is prepared, and after high-pressure sterilization at 121°C for 15 minutes, it is cooled to about 60°C. After adding a certain concentration of antibiotic (A) or photosensitive material X, it is uniformly mixed. Then, the culture medium is poured into the neck 110 and covered with the upper cover 300 for natural cooling and solidification.

[0035] Then, the upper cover 300 is removed, a certain volume and concentration of fluorescent bacteria (with resistance to antibiotic A) are added, and the upper cover 300 is covered again after uniform coating by the smearing piece 400. If the photosensitive material is not heat-resistant, a certain volume of photosensitive material X can be added on the solid culture medium first, and then the fluorescent bacteria are uniformly coated after being absorbed by the smearing piece 400.

[0036] Then, the whole device is taken to an outdoor or fixed culture place, and then the upper cover 300 and the separator 200 are removed. The fluorescent bacterial colonies are grown and counted, and the mixed bacteria are excluded.

[0037] In this embodiment, the smearing piece 400 includes a handle 410 and a smearing part 420 connected to the handle 410. In use, the smearing part 420 can be operated by the handle 410. The smearing part 420 can increase the smearing area. In specific implementation, the width of the smearing part 420 can be preferably 1-2 cm.

[0038] In this embodiment, a side wall of the neck 110 is provided with a jack that communicates with the inner cavity, and the separator 200 is a plug 210. The end of the plug 210 can be inserted into the neck 110 from outside the neck 110 through the jack to block the passage of the neck 110.

[0039] In use, when the upper cavity and the lower cavity need to be connected, the plug 210 can be pulled out, and conversely, when the upper cavity and the lower cavity need to be separated, the plug 210 can be inserted.

[0040] Second embodiment:

[0041] See Figure 5 Different from the first embodiment, the second embodiment is characterized in that:

[0042] In the embodiment, the partition 200 comprises a rotating shaft 220 and a baffle 230. The rotating shaft 220 is horizontally arranged in the bottle neck 110 and rotatable, and the baffle 230 is sleeved and fixed on the rotating shaft 220. The baffle 230 is shaped to match the cross-sectional dimension of the inner cavity of the bottle neck 110, and the baffle 230 can block the passage of the bottle neck 110 when the baffle 230 is rotated to a horizontal state.

[0043] In use, when the baffle 230 is in a horizontal state, the passage of the bottle neck 110 is blocked, and when the rotating shaft 220 and the baffle 230 are rotated by a certain angle, the baffle 230 is inclined, and the passage of the bottle neck 110 is opened. In specific implementation, the end of the rotating shaft 220 can be extended to the outside of the bottle neck 110 to facilitate the rotation of the baffle 230.

[0044] The application is suitable for a whole experimental device for verifying the antibacterial effect of photosensitive antibacterial material. In the cultivation process, the lower part of the upper cavity is in communication with the experimental bottle, so that water and nutrients can be continuously supplied. In addition, the upper cover can be removed, so that the effect of light irradiation is not affected. Therefore, the problem of maintaining light irradiation, water and nutrients during the cultivation of photosensitive material and microorganisms can be effectively solved.

[0045] The above embodiments are only used to illustrate the technical solutions of the application, but not limit the application. Although the application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that the technical solutions recorded in the foregoing embodiments can be modified, or some or all of the technical features can be replaced by equivalent substitutes. These modifications or replacements do not change the essence of the corresponding technical solutions, and they should be covered in the scope of the claims and the specification of the application.

Claims

1. A whole set experiment device suitable for bacteriostatic verification of photosensitive antibacterial material, characterized in that, The utility model relates to a culture bottle, comprising: an experimental bottle, the bottle neck of which extends vertically upward; a partition arranged in the bottle neck, which divides the passage of the bottle neck into an upper cavity and a lower cavity that are not connected; an upper cover, which is a detachable cover arranged on the top of the bottle neck; and an application part, which is used for uniformly applying photosensitive material and microorganisms on the solid culture medium in the upper cavity. The sidewall of the bottle neck is provided with a bushing communicating with the inner cavity, and the partition is a plug, the end of which can be inserted into the bottle neck through the bushing outside the bottle neck to divide the passage of the bottle neck into an upper cavity and a lower cavity that are not connected.

2. The whole experimental device suitable for the verification of the antibacterial property of photosensitive antibacterial material according to claim 1, characterized in that, The partition comprises a rotating shaft and a baffle, the rotating shaft is arranged transversely and rotatably in the bottle neck, the baffle is fixedly sleeved on the rotating shaft, the shape of the baffle is matched with the cross-sectional dimension of the inner cavity of the bottle neck, and when the baffle rotates to a horizontal state, the baffle can block the passage of the bottle neck. 3.The whole experimental device suitable for the verification of the antibacterial property of photosensitive antibacterial material according to claim 1, wherein, The end of the rotating shaft extends to the outside of the bottle neck.

4. The whole experimental device suitable for the verification of the antibacterial property of photosensitive antibacterial material according to claim 3, characterized in that, The application part comprises a handle and an application part connected to the handle.

5. The whole experimental device suitable for the verification of the antibacterial property of photosensitive antibacterial material according to claim 1, characterized in that, The application part is triangular, one end of the application part is connected to the handle.

6. The whole experimental device suitable for the verification of the antibacterial property of photosensitive antibacterial material according to claim 5, characterized in that, The width of the application part is 1-2 cm.

7. The whole experimental apparatus suitable for the verification of the antibacterial activity of photosensitive antibacterial materials according to claim 5 or 6, characterized in that, The outer wall of the experimental bottle is provided with a scale along its height. 8.The whole experimental device suitable for the verification of the antibacterial property of photosensitive antibacterial material according to claim 1, wherein, The outer dimension of the experimental bottle is a conical body with a large bottom and a small top. 9.The whole experimental device suitable for the verification of the antibacterial property of photosensitive antibacterial material according to claim 1, wherein, ​

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