Automatic culture and detection equipment for total number of bacterial colonies

Abstract

The utility model discloses equipment for automatically culturing and detecting the total number of bacterial colonies. Comprising a sterile assembly for forming a sterile cavity, an agar culture dish for containing agar, and a heating and melting assembly which is arranged in the sterile cavity and is used for heating the agar in the agar culture dish to a molten state, the sample introduction and uniform mixing assembly is arranged in the sterile cavity and is used for conveying a sample into an agar culture dish and uniformly mixing the sample and agar liquid; the constant-temperature culture assembly is arranged in the sterile cavity and is used for performing constant-temperature culture on a uniform mixture of the sample and the agar liquid; the visual identification assembly is arranged in the sterile cavity; the transfer assembly is arranged in the sterile cavity; compared with the prior art, the agar in the agar culture dish is heated to a molten state through the heating and melting assembly, so that the agar liquid does not need to be stored at constant temperature, bacterial colony culture is performed through the constant-temperature culture assembly, and the device is small in constant-temperature space, high in energy efficiency ratio, low in detection cost, high in practicability and suitable for wide popularization and application.

Description

TECHNICAL FIELD

[0001] The utility model relates to microorganism detection technical field, especially, relate to a kind of total number of colony automatic culture detection equipment. BACKGROUND

[0002] In various water bodies, often contain organic matter for the growth of various microorganisms, people's life cannot leave water resources, therefore, to protect people's physical and mental health, water microorganism needs to be detected, and total number of colony is the overall indicative index of evaluation water microorganism pollution degree, and it is an important index for evaluating water sanitation. At present, the commonly used detection method of total number of colony in water is plate counting method, and its principle is that microorganisms in water sample are fully dispersed into single cells after being diluted. A certain amount of diluted sample liquid is coated on the plate, and after cultivation, single cell growth and reproduction form visible colonies with naked eyes, that is, a single colony represents a single cell in the original water sample. Count the number of colonies, and the number of bacteria in the sample can be converted according to the dilution multiple and sampling inoculation amount. The method involves water sample injection, agar injection, plate shaking and other operations, and the operation process is easy to introduce pollution and requires high detection environment, but microorganism experiments often bring interference factors due to environment, reagent, vessel and other reasons, resulting in large deviation of measurement results.

[0003] To eliminate interference factors and improve the reliability of measurement results, Chinese invention patent application CN118638617A discloses an intelligent device and method for automatically detecting total number of colony in water and application. The sample bottle to be detected is placed on the sample bottle tray, the empty plate is placed on the second plate storage rotating disc, and the agar liquid in the agar liquid storage mechanism is kept at 45℃. The sample bottle is grabbed by the three-axis mechanical arm to the sample bottle tray, the sample bottle tray is fixed and shaken, the bottle cap is opened with the three-axis mechanical arm. The six-axis mechanical arm grabs the empty plate to the plate tray, and the appropriate amount of agar liquid and sample water is taken to the empty plate by the pipette, and the plate tray is shaken to wait for the agar liquid to solidify. The AGV device transfers the plate to the incubator according to the preset route for cultivation. Aerobic bacteria (staphylococcus, escherichia coli and streptococcus) are cultured at 33℃ for 5 days, and mold is cultured at 23℃ for 7 days. After cultivation, the total number of colonies is automatically counted by image recognition technology.

[0004] However, in the above device, the agar liquid needs to be kept at 45℃ all the time, and the microorganism culture period is relatively long, resulting in large overall energy consumption and high detection cost. UTILITY MODEL CONTENT

[0005] The utility model provides a kind of total number of colony automatic culture detection equipment to solve the technical problems of large energy consumption and high detection cost of the existing total number of colony culture detection device.

[0006] According to one aspect of the present application, an automatic colony count culture and detection device is provided, comprising a sterile assembly for forming a sterile cavity, an agar culture dish for containing agar, a heating and melting assembly arranged in the sterile cavity for heating the agar in the agar culture dish to a molten state, a sample feeding and mixing assembly arranged in the sterile cavity for feeding a sample into the agar culture dish and mixing the sample and agar liquid, a constant temperature culture assembly arranged in the sterile cavity for constant temperature culture of the mixture of the sample and agar liquid, a visual recognition assembly arranged in the sterile cavity for recognizing and counting the number of colonies on the agar culture dish, and a transfer assembly arranged in the sterile cavity for moving the agar culture dish between the heating and melting assembly, the sample feeding and mixing assembly, the constant temperature culture assembly and the visual recognition assembly.

[0007] As a further improvement of the above technical solution:

[0008] Further, the heating and melting assembly comprises a heating shell arranged in the sterile cavity, a heating door movably arranged on the heating shell for forming a heating chamber with the heating shell, a door driving member connected to the heating door at a movable end for driving the heating door to open or close the heating chamber, and a melting heater arranged in the heating chamber for heating the agar in the agar culture dish to a molten state.

[0009] Further, the heating door is movably arranged on the heating shell in a vertical direction, and the melting heater comprises an upper heater arranged on an upper wall of the heating chamber and a lower heater arranged on a lower wall of the heating chamber.

[0010] Further, the constant temperature culture assembly comprises a culture shell, a culture door movably arranged on the culture shell, and a culture constant temperature member arranged in the culture shell.

[0011] Further, the constant temperature culture assembly further comprises a rotating disc arranged in the culture shell, a plurality of placement frames for carrying the agar culture dish arranged at intervals along the circumference of the rotating disc, and a culture driving member connected to the rotating disc at a movable end for driving the rotating disc to rotate.

[0012] Further, the sample feeding and mixing assembly comprises a mixing tray for carrying the agar culture dish, a sample feeding member for feeding the sample into the agar culture dish, and a mixing driving member connected to the mixing tray at a movable end for driving the mixing tray to rotate to mix the sample and agar liquid.

[0013] Further, the culture detection device further comprises a light shielding shell, a placing table for placing the agar culture dish and being arranged on the light shielding shell in a pushable and pullable manner, a push-pull driving member for pushing and pulling the placing table to a specified position and being connected with the placing table in a movable manner, and a light emitting member for providing light conditions required for photographing and being arranged in the light shielding shell.

[0014] Further, the transferring assembly comprises a three-axis mechanical arm, a connecting plate arranged on a movable end of the three-axis mechanical arm, a flat supporting plate for supporting the agar culture dish and being arranged on the connecting plate, a vertical moving member movably arranged on the connecting plate in a vertical direction, and a vacuum suction disc for sucking the agar culture dish and being connected with the movable end of the vertical moving member.

[0015] Further, the sterilization assembly comprises a mounting shell with an openable and closable cavity, a sterilization member for sterilizing and disinfecting the openable and closable cavity to form a sterile cavity and being arranged in the mounting shell, and a pressure regulating member for regulating the internal pressure of the sterile cavity to maintain the sterile state and being arranged on the mounting shell.

[0016] Further, the detection device further comprises a collecting rack for accommodating the agar culture dish and being arranged in the sterile cavity.

[0017] The utility model has the following beneficial effects:

[0018] The automatic colony total number culture detection equipment of the utility model, when carrying out colony total number culture detection of sample, through sterile assembly forms sterile cavity, places agar culture dish containing agar in heating melting assembly, heats agar in agar culture dish to melting state, then moves agar culture dish in heating melting assembly to sample feeding and mixing assembly through transfer assembly, feeds sample to agar culture dish through sample feeding and mixing assembly and mixes sample and agar liquid, then moves agar culture dish in sample feeding and mixing assembly to constant temperature culture assembly through transfer assembly, carries out constant temperature culture to mixture of sample and agar liquid, finally moves agar culture dish in constant temperature culture assembly to visual identification assembly through transfer assembly, identifies and counts colony number on agar culture dish, so as to complete colony total number culture detection of sample, through cooperation of sterile assembly, agar culture dish, heating melting assembly, sample feeding and mixing assembly, constant temperature culture assembly, visual identification assembly and transfer assembly, realizes whole automatic operation of agar melting, sample and agar liquid mixing, colony culture and colony identification counting, and during whole culture detection process, carries out in sterile cavity, so as to improve detection efficiency, eliminate interference factors to the maximum, improve reliability of detection result, and compared with prior art, through heating melting assembly, agar in agar culture dish is heated to melting state, so that agar liquid does not need to be kept at constant temperature, then carries out colony culture through constant temperature culture assembly, constant temperature space is small, energy efficiency ratio is high, detection cost is low, practicality is strong, is suitable for being widely promoted and applied.

[0019] In addition to the purposes, features and advantages described above, the utility model has other purposes, features and advantages. The utility model will be further explained in detail below with reference to the drawings. ACCURACY

[0020] The accompanying drawings, which form a part of this application, are included to provide a further understanding of the application and are incorporated in and constitute a part of this application. The embodiments illustrated in the drawings are provided to explain the present application and are not intended to limit the present application. In the drawings:

[0021] Figure 1 It is the structure schematic diagram of automatic colony total number culture detection equipment of preferred embodiment of the utility model;

[0022] Figure 2 It is the structure schematic diagram of heating melting assembly in automatic colony total number culture detection equipment of preferred embodiment of the utility model;

[0023] Figure 3 It is the structure schematic diagram of sample feeding and mixing assembly in automatic colony total number culture detection equipment of preferred embodiment of the utility model;

[0024] Figure 4Is the structure diagram of the overturning assembly in the automatic colony count culture and detection equipment of the preferred embodiment of the utility model

[0025] Figure 5 Is the structure diagram of the constant temperature culture assembly in the automatic colony count culture and detection equipment of the preferred embodiment of the utility model

[0026] Figure 6 Is the structure diagram of the constant temperature culture assembly in the automatic colony count culture and detection equipment of the preferred embodiment of the utility model

[0027] Figure 7 Is the structure diagram of the visual identification assembly in the automatic colony count culture and detection equipment of the preferred embodiment of the utility model

[0028] Figure 8 Is the internal structure diagram of the visual identification assembly in the automatic colony count culture and detection equipment of the preferred embodiment of the utility model

[0029] Figure 9 Is the structure diagram of the collection frame in the automatic colony count culture and detection equipment of the preferred embodiment of the utility model

[0030] Figure 10 Is the structure diagram of the moving assembly in the automatic colony count culture and detection equipment of the preferred embodiment of the utility model

[0031] Figure 11 Is the structure diagram of the sterilization part in the automatic colony count culture and detection equipment of the preferred embodiment of the utility model

[0032] Figure 12 Is the structure diagram of the pressure adjustment in the automatic colony count culture and detection equipment of the preferred embodiment of the utility model

[0033] Figure 13 Is the partial structure diagram of the pressure adjustment in the automatic colony count culture and detection equipment of the preferred embodiment of the utility model

[0034] Legend:

[0035] 100. Aseptic components; 110. Mounting housing; 120. Sterilization components; 121. Lamp cover; 122. UV lamp; 130. Pressure regulating components; 131. Chamber body; 132. Chamber lid; 133. Dustproof net; 134. Positive pressure fan; 135. Filter screen; 200. Agar culture dish; 300. Heating and melting components; 301. Heating housing; 302. Heating valve; 303. Upper heater; 304. Lower heater; 305. Valve actuator; 306. Insulation layer; 400. Sample injection and mixing components; 401. Mixing tray; 402. Mixing actuator; 403. Syringe; 404. Pressure plate; 405. Injection plunger; 500. Constant pressure... 501. Culture shell; 502. Culture valve; 503. Rotating disk; 504. Placement frame; 505. Culture drive component; 506. Culture heater; 507. Circulating fan; 508. Photoelectric switch baffle; 601. Light-shielding shell; 602. Placement stage; 603. Push-pull drive component; 604. Light-emitting component; 605. Visual recognition component; 700. Transfer component; 701. Three-axis robotic arm; 702. Connecting plate; 703. Flat support plate; 704. Vertical moving component; 705. Vacuum suction cup; 800. Tilting component; 801. Tilting base; 802. Tilting motor; 803. Tilting box; 900. Collection rack. Detailed Implementation

[0036] The embodiments of the present invention will be described in detail below with reference to the accompanying drawings. However, the present invention can be implemented in many different ways as defined and covered below.

[0037] like Figure 1 As shown, the automated colony count culture and detection device of this embodiment includes a sterile component 100 for forming a sterile cavity, an agar culture dish 200 for holding agar, a heating and melting component 300 arranged in the sterile cavity for heating the agar in the agar culture dish 200 to a molten state, a sample injection and mixing component 400 arranged in the sterile cavity for transporting the sample into the agar culture dish 200 and mixing the sample and agar solution, a constant temperature culture component 500 arranged in the sterile cavity for constant temperature culture of the mixture of sample and agar solution, a visual recognition component 605 arranged in the sterile cavity for identifying and counting the number of colonies on the agar culture dish 200, and a transfer component 700 arranged in the sterile cavity for moving the agar culture dish 200 between the heating and melting component 300, the sample injection and mixing component 400, the constant temperature culture component 500, and the visual recognition component 605.

[0038] like Figure 1As shown, specifically, the total number of colonies automatic culture and detection equipment of the utility model, in the total number of colonies culture and detection of sample, through sterile assembly 100 forms sterile cavity, put agar plate 200 containing agar into heating melting assembly 300, to heat agar in agar plate 200 to melt state, then through transfer assembly 700, agar plate 200 in heating melting assembly 300 is moved to sample mixing assembly 400, through sample mixing assembly 400, sample is delivered to agar plate 200 and makes sample and agar liquid mix, again through transfer assembly 700, agar plate 200 in sample mixing assembly 400 is moved to constant temperature culture assembly 500, to carry out constant temperature culture to the mixture of sample and agar liquid, finally through transfer assembly 700, agar plate 200 in constant temperature culture assembly 500 is moved to visual identification assembly 605, to identify the number of colonies on agar plate 200, to complete the total number of colonies culture and detection of sample, the scheme through sterile assembly 100, agar plate 200, heating melting assembly 300, sample mixing assembly 400, constant temperature culture assembly 500, visual identification assembly 605 and transfer assembly 700 cooperate, realize the whole automatic operation of agar melting, sample and agar liquid mixing, colony culture and colony identification counting, and in the whole culture and detection process, in sterile cavity, to improve the detection efficiency, and the maximum degree of interference factor is eliminated, improve the reliability of detection result, and compared with prior art, through heating melting assembly 300, agar in agar plate 200 is heated to melt state, so that agar liquid does not need to be kept at constant temperature, again through constant temperature culture assembly 500, colony culture, constant temperature space is small, energy efficiency ratio is high, detection cost is low, practicality is strong, is suitable for extensive promotion and application.

[0039] As Figure 1 As shown in the embodiment, the agar plate 200 includes a plate body and a plate cover, and the detection equipment further includes a turnover assembly 800 arranged in the sterile cavity. When the agar in the agar plate 200 is heated to a melt state, the agar plate 200 is moved to the sample mixing assembly 400 by the transfer assembly 700. The sample mixing assembly 400 delivers the sample to the agar plate 200 and mixes the sample and the agar liquid. The transfer assembly 700 transfers the plate cover to the turnover assembly 800 and vertically turns over the plate cover. The transfer assembly 700 transfers the plate body to the constant temperature culture assembly 500 after the agar liquid and the sample in the plate body are mixed and solidified. The plate body is first transferred to the turnover assembly 800 for vertical turning over, and then is transferred to the constant temperature culture assembly 500 and is buckled on the plate cover. In this way, the growth and counting detection of microorganisms during the culture process can be avoided.

[0040] AsFigure 4 As shown, in the embodiment, the overturning assembly 800 comprises an overturning base 801, an overturning motor 802 arranged in the overturning base 801, an overturning box 803 connected with the overturning shaft of the overturning motor 802, and a micro switch arranged on the overturning base 801 and close to the overturning shaft. The overturning box 803 is used for vertically limiting and circumferentially limiting the culture dish body and the culture dish cover body, respectively. After the transfer assembly 700 transfers the culture dish body or the culture dish cover body into the overturning box 803, the overturning motor 802 rotates to drive the overturning box 803 to rotate. The micro switch stops rotating after sensing that the overturning shaft rotates 180°, so as to realize vertical overturning of the culture dish body or the culture dish cover body by 180°. Then, the transfer assembly 700 transfers the culture dish body or the culture dish cover body to the next process.

[0041] In the embodiment, the detection device further comprises a control system electrically connected with the sterile assembly 100, the heating and melting assembly 300, the sample feeding and mixing assembly 400, the constant-temperature culture assembly 500, the visual recognition assembly 605 and the transfer assembly 700, so as to control the sterile assembly 100, the heating and melting assembly 300, the sample feeding and mixing assembly 400, the constant-temperature culture assembly 500, the visual recognition assembly 605 and the transfer assembly 700 to work cooperatively and realize automatic operation. Optionally, the control system comprises a PLC controller and a control panel electrically connected with the PLC controller. It should be understood that the specific structure of the PLC controller belongs to the common technical knowledge of those skilled in the art, and will not be described in detail here.

[0042] As shown in FIG. 6, in the embodiment, the heating and melting assembly 300 comprises a heating shell 301 arranged in the sterile cavity, a heating door 302 movably arranged on the heating shell 301 and used for forming a heating chamber together with the heating shell 301, a door driving member 305 having a movable end connected with the heating door 302 and used for driving the heating door 302 to open or close the heating chamber, and a melting heating member arranged in the heating chamber and used for heating the agar in the agar culture dish 200 to a molten state. Figure 2 Specifically, when the total number of colonies in the sample is cultured and detected, the door driving member 305 drives the heating door 302 to open the heating chamber, so as to transfer the agar culture dish 200 into the heating chamber. Then, the door driving member 305 drives the heating door 302 to open and close the heating chamber again. The melting heating member heats the agar in the agar culture dish 200 to a molten state, which is convenient for subsequent mixing of the sample and the agar liquid. Optionally, the door driving member 305 is an electric push rod.

[0043] As shown in FIG. 6, in the embodiment, the heating and melting assembly 300 comprises a heating shell 301 arranged in the sterile cavity, a heating door 302 movably arranged on the heating shell 301 and used for forming a heating chamber together with the heating shell 301, a door driving member 305 having a movable end connected with the heating door 302 and used for driving the heating door 302 to open or close the heating chamber, and a melting heating member arranged in the heating chamber and used for heating the agar in the agar culture dish 200 to a molten state. Figure 2As shown, in this embodiment, the heating valve 302 is vertically and movably arranged on the heating housing 301. The melting heating element includes an upper heater 303 arranged on the upper wall of the heating chamber and a lower heater 304 arranged on the lower wall of the heating chamber. Specifically, the heating valve 302 is vertically movably arranged, so it will not interfere with the installation and operation of the upper heater 303 or the lower heater 304. After the solidified agar culture dish 200 is placed in the heating chamber, the upper heater 303 operates first to heat the culture dish cover, and then the lower heater 304 operates until the temperature in the heating chamber reaches the set temperature. This heating method ensures that the water vapor generated by the agar culture dish 200 during heating will not condense due to contact with the cold culture dish cover, thus affecting the subsequent culture and testing process. Optionally, the set temperature is 85℃-100℃.

[0044] Optionally, the upper heater 303 is either a heating plate or a PI heating film. Optionally, the lower heater 304 is either a heating plate or a PI heating film.

[0045] like Figure 2 As shown, in this embodiment, the upper heater 303 is equipped with an upper temperature sensor, and the lower heater 304 is equipped with a lower temperature sensor. When heating the agar culture dish 200, the upper heater 303 first heats to the set temperature, and then the lower heater 304 starts working. When the upper temperature sensor and the lower temperature sensor detect that the temperature has reached the set temperature, the upper heater 303 and the lower heater 304 stop heating and maintain the temperature in the heating chamber at the set temperature until the transfer component 700 removes the agar culture dish 200. Optionally, before heating the agar culture dish 200, the upper heater 303 and the lower heater 304 heat simultaneously. When the upper and lower temperature sensors detect that the temperature has reached a first preset temperature, and maintain the temperature at the first preset temperature, the transfer assembly 700 transfers the unheated agar culture dish to the heating chamber. Then, the upper heater 303 heats until the upper temperature sensor detects that the temperature has reached a second preset temperature. The upper heater 303 then stops heating and maintains the temperature at the second preset temperature. After a first preset time of constant temperature, the lower heater 304 heats until the lower temperature sensor detects that the temperature has reached the second preset temperature. The upper heater 303 and the lower heater 304 then stop heating and maintain the temperature at the second preset temperature. After a second preset time of constant temperature, the transfer assembly 700 can remove the agar culture dish 200. Optionally, in one embodiment, the temperature is set to 85℃-100℃. Optionally, in another embodiment, the first preset temperature is 45-55℃, the second preset temperature is 85℃-100℃, the first preset time is 3-10 minutes, and the second preset time is 2-5 minutes.

[0046] like Figure 2As shown, in the embodiment, the heating and melting assembly 300 further comprises a heat insulation layer 306 arranged in the heating shell 301, so as to keep the temperature of the heating cavity constant within the preset temperature range through the heat insulation layer 306.

[0047] As shown in the figures, Figure 5 and Figure 6 As shown, in the embodiment, the constant temperature culture assembly 500 comprises a culture shell 501, a culture door 502 movably arranged on the culture shell 501, and a culture constant temperature component arranged in the culture shell 501. Specifically, in the initial state, the culture door 502 is opened or closed, and the culture constant temperature component works to maintain the culture shell 501 within the set culture temperature. After the transfer assembly 700 transfers the agar culture dish 200 to the upper side of the culture shell 501, the culture door 502 is opened to place the agar culture dish 200 in the culture shell 501, and then the culture door 502 is closed for constant temperature culture for the set culture time.

[0048] As shown in the figures, Figure 5 and Figure 6 As shown, in the embodiment, the culture constant temperature component comprises a culture heater 506, a heating fan, and a circulating fan 507. The culture heater 506 is used for heating, the heating fan is used for blowing hot air, and the circulating fan 507 is used for circulating the air in the culture shell 501, so as to maintain the set culture temperature. Optionally, the set culture temperature is 37±1℃.

[0049] As shown in the figures, Figure 5 and Figure 6 As shown, in the embodiment, the constant temperature culture assembly 500 further comprises a rotating disc 503 arranged in the culture shell 501, a plurality of placement frames 504 for carrying the agar culture dish 200 arranged along the circumference of the rotating disc 503, and a culture driving component 505 movably connected to the rotating disc 503 and used for driving the rotating disc 503 to rotate. Specifically, the culture driving component 505 drives the rotating disc 503 to rotate, so that the idle placement frame 504 is arranged corresponding to the culture door 502. After the culture door 502 is opened, a batch of agar culture dishes 200 can be placed on the placement frame 504. Then, the culture driving component 505 drives the rotating disc 503 to rotate, so that another idle placement frame 504 is arranged corresponding to the culture door 502. Another batch of agar culture dishes 200 can be placed on the placement frame 504. The above process is repeated until there is no idle placement frame 504. In this way, a plurality of batches of samples can be cultured simultaneously through the plurality of placement frames 504, which greatly improves the culture detection efficiency. Optionally, the culture driving component 505 is a driving motor.

[0050] As shown in the figures, Figure 5As shown, in this embodiment, a plurality of photoelectric switch baffles 508 are arranged on the rotating disk 503. The photoelectric switch baffles 508 are arranged in correspondence with the placement frame 504, so that the corresponding placement frame 504 can be stopped directly below the culture door for the placement and removal of the agar culture dish 200.

[0051] It should be understood that, in actual culture and testing, in order to improve the reliability of the test results, in addition to the test water sample, there are also parallel water samples and blank water samples. The same placement frame 504 can stack three agar culture dishes 200 of the same batch of test water samples, parallel water samples, and blank water samples in sequence.

[0052] like Figure 3 As shown, in this embodiment, the sample injection and mixing assembly 400 includes a mixing tray 401 for supporting the agar culture dish 200, a sample delivery component for delivering the sample into the agar culture dish 200, and a mixing drive component 402 whose movable end is connected to the mixing tray 401 for driving the mixing tray 401 to rotate so that the sample and agar solution are mixed. Specifically, after the transfer component 700 transfers the agar culture dish 200 onto the mixing tray 401, and then the dish cap is held and transferred onto the flipping component 800, the mixing drive 402 rotates the mixing tray 401 so that the agar culture dish 200 is below the sample loading component. The sample loading component then adds a quantitative sample into the agar culture dish 200. The mixing drive 402 then rotates the mixing tray 401 again until the sample and agar solution are evenly mixed. The mixing drive 402 rotates the mixing tray 401 to move the agar culture dish 200 to its initial position. After the mixture of sample and agar solution solidifies, the transfer component 700 removes the agar culture dish 200. Optionally, the sample injection mixing component 400 also includes a photoelectric sensor disposed on the mixing tray 401, which, in conjunction with the mixing drive 402, moves the agar culture dish 200 to the designated position.

[0053] like Figure 3 As shown, in this embodiment, the sample addition component includes a syringe 403 arranged vertically, a pressure plate 404 for pressing the syringe 403, and an injection electric plunger 405 whose movable end is connected to the pressure plate 404. The injection electric plunger 405 drives the pressure plate 404 to move vertically, thereby realizing the addition of the sample.

[0054] like Figure 7 and Figure 8As shown, in this embodiment, the culture detection device comprises a light-shielded shell 601, a placing table 602 arranged on the light-shielded shell 601 and used for placing the agar culture dish 200, a push-pull driving member 603 connected with the placing table 602 and used for pushing and pulling the placing table 602 to a specified position, a light emitting member 604 arranged in the light-shielded shell 601 and used for providing the light condition required for photographing, and a visual recognition assembly 605 arranged on the light-shielded shell 601. Specifically, the placing table 602 is pushed by the push-pull driving member 603 to move outside the light-shielded shell 601, and then the agar culture dish 200 after culture is placed on the placing table 602. The placing table 602 is pulled by the push-pull driving member 603 to move inside the light-shielded shell 601. The light emitting member 604 works to provide the light condition required for photographing. The visual recognition assembly 605 photographs the agar culture dish 200, and then processes the photo to identify and count the number of bacteria on the agar culture dish 200. Finally, the placing table 602 is pushed by the push-pull driving member 603 to move outside the light-shielded shell 601, and the transfer assembly 700 transfers the agar culture dish 200 to the next process. Optionally, the light emitting member 604 comprises an LED lamp and a soft light lamp. Optionally, the visual recognition assembly 605 comprises a camera. Optionally, the push-pull driving member 603 is an electric push rod. It should be understood that the light-shielded shell 601 and the light emitting member 604 cooperate to provide a good photographing environment, so as to improve the clarity of the photo and thus improve the accuracy of the detection result.

[0055] As shown, Figure 10 In this embodiment, the transfer assembly 700 comprises a three-axis mechanical arm 701, a connecting plate 702 arranged on the movable end of the three-axis mechanical arm 701, a flat supporting plate 703 arranged on the connecting plate 702 and used for lifting the agar culture dish 200, a vertical moving member 704 movably arranged on the connecting plate 702 in the vertical direction, and a vacuum suction cup 705 connected with the movable end of the vertical moving member 704 and used for sucking the agar culture dish 200. Specifically, the connecting plate 702 is moved to a specified position in space by the three-axis mechanical arm 701, so that the agar culture dish can be lifted by the flat supporting plate 703. At this time, the vertical moving member 704 drives the vacuum suction cup 705 to move away from the flat supporting plate 703. When the vacuum suction cup 705 needs to suck the agar culture dish 200, the vertical moving member 704 drives the vacuum suction cup 705 to move below the flat supporting plate 703, so as to facilitate the vacuum suction cup 705 to suck the agar culture dish 200. It should be understood that the flat supporting plate 703 is composed of a plurality of flat plates arranged in the horizontal direction and spaced apart. Adjacent two flat plates have a gap for the vacuum suction cup 705 to pass through. Optionally, the vertical moving member 704 is an electric push rod.

[0056] As shown, Figure 1As shown, in the embodiment, the sterile assembly 100 comprises a mounting housing 110 with an openable and closable cavity, a sterilization member 120 arranged in the mounting housing 110 for sterilizing and disinfecting the openable and closable cavity to form a sterile cavity, and a pressure regulating member 130 arranged on the mounting housing 110 for regulating the internal pressure of the sterile cavity to maintain the sterile state. Specifically, when performing the total number of colonies culture detection in the sample, the sterilization member 120 sterilizes and disinfects the openable and closable cavity to form the sterile cavity, and then the regulating member regulates the internal pressure of the sterile cavity to maintain the sterile state of the sterile cavity, thereby improving the reliability of the detection result. Optionally, before the transfer assembly 700 transfers the agar culture dish 200, the vacuum chuck 705 on the transfer assembly 700 can be moved into the sterilization member 120 to prevent the vacuum chuck 705 from sucking the agar culture dish 200 and causing contamination to the agar culture dish 200, thereby affecting the detection result.

[0057] As shown, Figure 11 In the embodiment, the sterilization member 120 comprises a lampshade 121 and a UV lamp 122 arranged in the lampshade 121 to sterilize and disinfect by UV light.

[0058] Optionally, in another embodiment, the sterilization member 120 adopts ozone sterilization, or adopts a combination of UV sterilization and ozone sterilization.

[0059] As shown, Figure 12 and Figure 13 In the embodiment, the pressure regulating member 130 comprises a box body 131, a box cover 132 covering the box body 131, a dust screen 133 arranged on the box body 131, a positive pressure fan 134 arranged in the box body 131, and a filter screen 135 arranged on the box body 131. The positive pressure fan 134 works to draw filtered air from the outside of the dust screen 133 into the inside of the box body 131, and then the filter screen 135 discharges sterile air into the sterile cavity to maintain the sterile state of the sterile cavity. Optionally, the filter screen 135 is a H13 high-efficiency filter screen.

[0060] As shown, Figure 9 In the embodiment, the detection device further comprises a collection rack 900 arranged in the sterile cavity for receiving the agar culture dish 200. Specifically, the collection rack 900 receives the culture dish cover body after culture and the culture dish body after photographic detection to clean out the agar culture dish 200 after detection for harmless treatment. It should be understood that the height of the baffle of the collection rack 900 can be increased to increase the stackable number of the agar culture dishes 200 according to actual detection needs.

[0061] As shown, Figures 1-13 In an embodiment, the working process of the total number of colonies automatic culture detection device is as follows:

[0062] The sterilization part 120 works for 30 minutes, the vacuum suction plate 705 on the moving assembly extends into the sterilization part 120 for sterilization for 30 minutes, and then the pressure regulating part 130 works to maintain the aseptic cavity in an aseptic state;

[0063] The valve driving part 305 opens the heating valve 302 to place the agar plate 200 into the inner cavity of the heating shell 301, and then the valve driving part 305 closes the heating valve 302. After the agar is melted into agar liquid, the valve driving part 305 opens the heating valve 302, and the transfer assembly 700 lifts the agar plate 200 by the flat supporting plate 703 and moves to the mixing tray 401. Then the transfer assembly 700 sucks the plate cover by the vacuum suction plate 705 and transfers to the turnover box 803 of the turnover assembly 800;

[0064] The mixing driving part 402 drives the mixing tray 401 to rotate, and the plate body is moved below the syringe 403. The injection electric push rod 405 pushes the syringe 403 to deliver the sample into the plate body. Then the mixing driving part 402 drives the mixing tray 401 to rotate, and the plate body is stopped at the initial placement position of the mixing tray 401 after the sample and agar liquid are mixed;

[0065] The turnover assembly 800 rotates the turnover box 803 by the turnover motor 802 to turn over the plate cover by 180°, and then opens the culture valve 502 to place the plate cover on the placement frame 504 by the transfer assembly 700;

[0066] After the sample and agar are mixed and solidified, the plate body is lifted by the flat supporting plate 703 of the transfer assembly 700 and transferred to the turnover box 803. After the plate body is turned over by 180°, the plate body is transferred to the culture shell 501 by the vacuum suction plate 705 of the transfer assembly 700 and covers the plate cover. Then the culture valve 502 is closed, and the culture thermostat works to maintain the culture shell 501 at a set culture temperature;

[0067] After the parallel samples and blank samples are treated by the same process and placed on the previous agar plate 200, the samples are incubated at a constant temperature for 48±2h. Then the culture valve 502 is opened, the plate body of the blank sample is moved to the extended placement table 602 by the vacuum suction plate 705 of the transfer assembly 700, and then the placement table 602 is retreated into the light shielding shell 601 to take a photo and count the number of bacteria on the plate body of the blank sample by the visual recognition assembly 605;

[0068] The blank sample culture dish cover is transferred to the collecting rack 900 by the vacuum chuck 705 in the transfer assembly 700, and then the photographed and counted blank sample culture dish body is transferred to the collecting rack 900 to be buckled with the blank sample culture dish cover, and then the parallel sample and the detection sample are sequentially processed by using the same process;

[0069] After the detection sample, the parallel sample and the blank sample are processed, each component is restored to the initial state, and the agar culture dish 200 on the collecting rack 900 is cleaned out and disposed of harmlessly.

[0070] The above only describes preferred embodiments of the present application and is not intended to limit the present application. For those skilled in the art, the present application can be variously changed and modified. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. An automatic colony count incubation detection apparatus, characterized by, The sterile assembly (100) for forming a sterile cavity, the agar culture dish (200) for containing agar, the heating melting assembly (300) for heating the agar in the agar culture dish (200) to a molten state, the sample feeding and mixing assembly (400) for feeding a sample into the agar culture dish (200) and mixing the sample and agar liquid, the constant temperature culture assembly (500) for constant temperature culture of the mixture of the sample and agar liquid, the visual recognition assembly (605) for recognizing and counting the number of colonies on the agar culture dish (200), and the transfer assembly (700) for moving the agar culture dish (200) between the heating melting assembly (300), the sample feeding and mixing assembly (400), the constant temperature culture assembly (500) and the visual recognition assembly (605) are arranged in the sterile cavity.

2. The colony count automated incubation detection apparatus of claim 1, wherein, The heating melting assembly (300) comprises a heating shell (301) arranged in the sterile cavity, a heating door (302) movably arranged on the heating shell (301) to form a heating chamber with the heating shell (301), a door driving member (305) connected to the heating door (302) and used for driving the heating door (302) to open or close the heating chamber, and a melting heating member arranged in the heating chamber and used for heating the agar in the agar culture dish (200) to a molten state.

3. The colony count automated incubation detection apparatus of claim 2, wherein, The heating door (302) is movably arranged on the heating shell (301) in the vertical direction, and the melting heating member comprises an upper heater (303) arranged on the upper wall of the heating chamber and a lower heater (304) arranged on the lower wall of the heating chamber.

4. The colony count automated incubation detection apparatus of claim 1, wherein, The constant temperature culture assembly (500) comprises a culture shell (501), a culture door (502) movably arranged on the culture shell (501), and a culture constant temperature member arranged in the culture shell (501).

5. The colony count automated incubation detection apparatus of claim 4, wherein, The constant temperature culture assembly (500) further comprises a rotating disc (503) arranged in the culture shell (501), a plurality of placing frames (504) for carrying the agar culture dish (200) arranged in the circumferential direction of the rotating disc (503), and a culture driving member (505) connected to the rotating disc (503) and used for driving the rotating disc (503) to rotate.

6. The colony count automated incubation detection apparatus according to any one of claims 1 to 5, wherein The sample feeding and mixing assembly (400) comprises a mixing tray (401) for carrying the agar culture dish (200), a sample feeding member for feeding a sample into the agar culture dish (200), and a mixing driving member (402) connected to the mixing tray (401) and used for driving the mixing tray (401) to rotate to mix the sample and agar liquid.

7. The colony count automated incubation detection apparatus according to any one of claims 1 to 5, wherein The culture detection device further comprises a light-shielded shell (601), a placing table (602) disposed on the light-shielded shell (601) and used for placing the agar culture dish (200), a push-pull driving member (603) connected with the placing table (602) and used for pushing and pulling the placing table (602) to a specified position, and a light-emitting member (604) disposed in the light-shielded shell (601) and used for providing light conditions required for photographing. The visual recognition assembly (605) is disposed on the light-shielded shell (601).

8. The colony count automated incubation detection apparatus according to any one of claims 1-5, wherein, The transfer assembly (700) comprises a three-axis mechanical arm (701), a connecting plate (702) disposed on a movable end of the three-axis mechanical arm (701), a flat supporting plate (703) disposed on the connecting plate (702) and used for supporting the agar culture dish (200), a vertical moving member (704) movably disposed on the connecting plate (702) in a vertical direction, and a vacuum suction cup (705) connected with a movable end of the vertical moving member (704) and used for sucking the agar culture dish (200).

9. The colony count automated incubation detection apparatus according to any one of claims 1-5, wherein, The sterile assembly (100) comprises a mounting shell (110) having an openable and closable cavity, a sterilization member (120) disposed in the mounting shell (110) and used for sterilizing and disinfecting the openable and closable cavity to form a sterile cavity, and a pressure regulating member (130) disposed on the mounting shell (110) and used for adjusting an internal pressure of the sterile cavity to maintain a sterile state.

10. The colony count automated incubation detection apparatus according to any one of claims 1-5, wherein, The detection device further comprises a collection rack (900) disposed in the sterile cavity and used for accommodating the agar culture dish (200).

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