Nucleic acid extraction and detection all-in-one machine

Abstract

The utility model belongs to the field of molecular detection, and particularly relates to a nucleic acid extraction and detection all-in-one machine which is characterized in that a sample treatment bin and an amplification detection bin which are mutually independent are arranged in a shell, and a sample treatment device and a PCR detection device are respectively arranged in the sample treatment bin and the amplification detection bin, so that physical partition of nucleic acid extraction and amplification analysis is realized; the sample treatment bin and the amplification detection bin are communicated through the sample transmission channel, and samples are transmitted between the sample treatment bin and the amplification detection bin by virtue of the cross-bin transmission assembly, so that aerosol pollution between nucleic acid extraction and amplification analysis is reduced, and the accuracy of a detection result is improved.

Description

Technical Field

[0001] This application relates to the field of molecular detection technology, and in particular to an integrated machine for nucleic acid extraction and detection. Background Technology

[0002] Molecular biology techniques used for gene screening or diagnosis mainly include molecular hybridization, fluorescence qualitative / quantitative analysis, and sequencing. These techniques primarily involve sample nucleic acid extraction, PCR reaction system preparation, and PCR amplification and detection. Traditional nucleic acid extraction and detection processes typically require multiple independent devices and cumbersome manual operations, which are not only inefficient but also prone to human error, affecting the accuracy of the test results.

[0003] Currently, although there are automated molecular diagnostic integrated machines, the devices for sample processing and nucleic acid extraction and the devices for PCR amplification and analysis are located in the same chamber and arranged close to each other, which leads to aerosol contamination and affects the accuracy of the test results. Utility Model Content

[0004] This application provides a nucleic acid extraction and detection integrated machine to reduce aerosol contamination between nucleic acid extraction and PCR amplification analysis in the integrated machine, thereby improving the accuracy of the detection results.

[0005] To achieve the above objectives, this application provides an integrated nucleic acid extraction and detection machine, comprising:

[0006] The casing has an independent sample processing compartment and an amplification detection compartment, which are connected by a sample transfer channel.

[0007] A cross-compartment transport component is provided throughout the sample transport channel, and the cross-compartment transport component is used to transport samples between the sample processing compartment and the amplification detection compartment.

[0008] A sample processing device, located within the sample processing chamber, is used for sample aliquoting, nucleic acid extraction, and construction of a PCR reaction system; and

[0009] A PCR detection device is installed inside the sample processing chamber and is used for PCR amplification detection.

[0010] Optionally, the PCR detection device includes a sealing device, a PCR detector, and a transfer device. The sealing device is used to seal the PCR plate after the PCR system construction is completed in the sample processing device; the PCR detector is used for PCR amplification and detection; and the transfer device is used to transfer the PCR plate between the cross-compartment transfer component, the sealing device, and the PCR detector.

[0011] Optionally, the interior of the amplification detection chamber is divided into a sealing chamber and a detection chamber by a partition. The partition is provided with a transmission port connecting the sealing chamber and the detection chamber. The sealing instrument is disposed in the sealing chamber, and the PCR detection instrument is disposed in the detection chamber.

[0012] The transfer device includes a first transfer mechanism, an intercavity transfer mechanism, and a second transfer mechanism. The intercavity transfer mechanism extends through the transfer port. The first transfer mechanism is disposed within the sealing cavity and is used to transfer the PCR plate between the cross-compartment transfer assembly, the sealing device, and the intercavity transfer mechanism. The second transfer mechanism is disposed within the detection cavity and is used to transfer the PCR plate between the intercavity transfer mechanism and the sealing device.

[0013] Optionally, the first transfer mechanism and the second transfer mechanism have the same structure, both including a linear module, a lifting module disposed on the linear module, and a gripper disposed on the lifting module. The gripper is used to grip the PCR plate, and the gripper moves up and down under the drive of the lifting module and moves laterally under the drive of the linear module.

[0014] Optionally, the cross-compartment transport assembly includes a linear module and a support mounted on the linear module, the support being used to support the PCR plate.

[0015] Optionally, the sample processing device includes a cupping module, a nucleic acid extraction module, a robotic arm module, and a solution preparation module. The cupping module is used to cup the samples, and the nucleic acid extraction module is used to extract nucleic acids from the cupped samples. The robotic arm module has a gripper and a pipette that can move within the sample processing chamber, and the solution preparation module is used to cooperate with the pipette to construct the PCR reaction system.

[0016] Optionally, the dispensing module includes a tube rack, a barcode scanner, a clamping assembly, and a deep-well plate holder. The tube rack is used to carry sample tubes, and the barcode scanner is used to input information about the sample tubes. The clamping assembly is used to hold the sample tubes, and the gripper can transfer the sample tubes from the tube rack to the clamping assembly and open the tube caps of the sample tubes in conjunction with the clamping assembly. The pipette is used to transfer the sample from the sample tubes to the deep-well plate on the deep-well plate holder for dispensing.

[0017] Optionally, the nucleic acid extraction module includes an 8-throughput nucleic acid extractor, a 16-throughput nucleic acid extractor, a 32-throughput nucleic acid extractor, a 64-throughput nucleic acid extractor, or a 96-channel nucleic acid extractor.

[0018] Optionally, the sample processing device further includes a consumable storage module, which is disposed in the sample processing chamber. The consumable storage module is provided with partitioned placement seats for storing deep well plates, PCR plates and pipette tips. The robotic arm module is also capable of transferring consumables between the consumable storage module and the dispensing module, between the consumable storage module and the nucleic acid extraction module, and between the consumable storage module and the solution preparation module.

[0019] Optionally, the nucleic acid extraction and detection integrated machine further includes an air filtration system, which is installed on the casing and used to filter the gas inside the sample processing chamber.

[0020] The beneficial effects of the nucleic acid extraction and detection integrated machine provided in this application are as follows: Compared with the prior art, the nucleic acid extraction and detection integrated machine of this application sets up independent sample processing chambers and amplification detection chambers in the machine casing, and sets the sample processing device and PCR detection device in the sample processing chamber and amplification detection chamber respectively, thereby realizing physical partitioning of nucleic acid extraction and amplification analysis. The sample processing chamber and the amplification detection chamber are connected through a sample transmission channel, and the sample is transferred between the sample processing chamber and the amplification detection chamber by means of a cross-chamber transmission component, which reduces aerosol contamination between nucleic acid extraction and amplification analysis and improves the accuracy of detection results. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] in:

[0023] Figure 1 This is a schematic diagram of the overall structure of a nucleic acid extraction and detection integrated machine according to an embodiment of this application;

[0024] Figure 2 yes Figure 1 The image shows the front view of the integrated nucleic acid extraction and testing machine.

[0025] Figure 3 This is a schematic diagram of a nucleic acid extraction and detection integrated machine after removing the casing, as shown in one embodiment of this application;

[0026] Figure 4 This is a schematic diagram of the cross-compartment transfer component in a nucleic acid extraction and detection integrated machine according to an embodiment of this application;

[0027] Figure 5This application illustrates a first transfer mechanism / second transfer mechanism in an integrated nucleic acid extraction and detection machine.

[0028] Explanation of key component symbols:

[0029] 100. Housing; 101. Sample processing chamber; 102. Amplification and detection chamber; 1020. Partition; 1021. Sealing chamber; 1022. Detection chamber; 103. Sample transfer channel; 104. Transfer port;

[0030] 200. Cross-warehouse transport component; 210. Linear module; 220. Bearing base;

[0031] 300. Sample processing device; 310. Dispensing cup module; 311. Tube rack; 312. Barcode scanner; 313. Clamping assembly; 314. Deep well plate rack; 320. Nucleic acid extraction module; 330. Robotic arm module; 331. Gripper; 332. Pipette;

[0032] 400. PCR detection device; 410. Sealing device; 420. PCR detection instrument; 430. Transfer device; 431. First transfer mechanism; 432. Intercavity transfer mechanism; 433. Second transfer mechanism; 4411. Linear module; 4412. Lifting module; 4413. Gripper;

[0033] 500. Consumables storage module. Detailed Implementation

[0034] To facilitate understanding of this application, a more complete description will be provided below with reference to the accompanying drawings. Preferred embodiments of this application are shown in the drawings. However, this application can be implemented in many other different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a thorough and complete understanding of the disclosure of this application.

[0035] It should be noted that when a component is referred to as being "fixed to" or "set on" another component, it can be directly on or indirectly on that other component. When a component is referred to as being "connected to" another component, it can be directly connected to or indirectly connected to that other component.

[0036] It should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0037] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, "multiple" means two or more, unless otherwise explicitly specified.

[0038] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.

[0039] Embodiments of this application provide a nucleic acid extraction and detection integrated machine, such as... Figures 1-3 As shown, the integrated nucleic acid extraction and detection machine includes a housing 100, a cross-compartment transfer assembly 200, a sample processing device 300, and a PCR detection device 400. The housing 100 has two independent sample processing compartments 101 and 102, connected by a sample transfer channel 103. The cross-compartment transfer assembly 200 extends through the sample transfer channel 103 and is used to transfer samples between the sample processing compartment 101 and the 102. The sample processing device 300 is located within the sample processing compartment 101 and is used for sample aliquoting, nucleic acid extraction, and PCR reaction system construction. The PCR detection device 400 is located within the sample processing compartment 101 and is used for PCR amplification detection.

[0040] In this embodiment, the nucleic acid extraction and detection integrated machine sets up independent sample processing chambers 101 and amplification detection chambers 102 in the casing 100, and respectively sets up sample processing device 300 and PCR detection device 400 in sample processing chamber 101 and amplification detection chamber 102, thereby realizing physical partitioning of nucleic acid extraction and amplification analysis. The sample processing chamber 101 and the amplification detection chamber 102 are connected by a sample transmission channel 103, and samples are transferred between the sample processing chamber 101 and the amplification detection chamber 102 by means of a cross-chamber transmission component 200, which reduces aerosol contamination between nucleic acid extraction and amplification analysis and improves the accuracy of detection results.

[0041] In one embodiment, such as Figures 2-3As shown, the PCR detection device 400 includes a sealing device 410, a PCR detector 420, and a transfer device 430. The sealing device 410 is used to heat seal the PCR plate after the PCR system construction is completed in the sample processing device 300; the PCR detector 420 is used for PCR amplification and detection; and the transfer device 430 is used to transfer the PCR plate between the cross-compartment transfer component 200, the sealing device 410, and the PCR detector 420.

[0042] During operation, the transfer device 430 picks up the PCR plate transferred from the cross-compartment transfer component 200 and transfers it to the sealing device 410. The sealing device 410 seals the opening of the PCR plate. After sealing, the transfer device 430 picks up the sealed PCR plate and transfers it to the PCR detector 420 for PCR amplification and detection.

[0043] Specifically, the sealing machine 410 is used for efficient sealing of PCR plates. The film roll replacement position is located at the front, the heating module is placed in the middle, and the film roll rotation and film take-up are driven by a stepper motor.

[0044] In one specific embodiment, such as Figures 1-2 As shown, the amplification detection chamber 102 is divided into a sealing chamber 1021 and a detection chamber 1022 by a partition 1020. The partition 1020 is provided with a transfer port 104 connecting the sealing chamber 1021 and the detection chamber 1022. The sealing device 410 is disposed in the sealing chamber 1021, and the PCR detection instrument 420 is disposed in the detection chamber 1022. The transfer device 430 includes a first transfer mechanism 431, an inter-chamber transfer mechanism 432, and a second transfer mechanism 433. The inter-chamber transfer mechanism 432 is disposed through the transfer port 104. The first transfer mechanism 431 is disposed in the sealing chamber 1021 and is used to transfer the PCR plate between the inter-chamber transfer assembly 200, the sealing device 410, and the inter-chamber transfer mechanism 432. The second transfer mechanism 433 is disposed in the detection chamber 1022 and is used to transfer the PCR plate between the inter-chamber transfer mechanism 432 and the sealing device 410.

[0045] The amplification and detection chamber 102 is divided into a sealing chamber 1021 for placing the sealing instrument 410 and a detection chamber 1022 for placing the PCR detection instrument 420 by using a partition 1020. This further physical partitioning reduces cross-contamination between the nucleic acid extraction area and the amplification and analysis area.

[0046] Preferably, the sample processing chamber 101, the sealing chamber 1021, and the detection chamber 1022 are arranged side by side in the same direction. It should be noted that the number of PCR detectors 420 can be set to multiple units according to the number of samples processed, and multiple PCR detectors 420 can be arranged in layers.

[0047] Furthermore, a storage space (not shown in the figure) is provided below the sealing cavity 1021. The storage space is used to store discarded PCR plates. The storage space is also equipped with a handling mechanism and a lifting device. The lifting device is used to transfer the discarded PCR plates from the sealing cavity 1021 to the storage space below, and then the handling mechanism is used to stack the discarded PCR plates in the storage space.

[0048] In one specific embodiment, such as Figures 2-3 and Figure 5 As shown, the first transfer mechanism 431 and the second transfer mechanism 433 have the same structure, both including a linear module 4411, a lifting module 4412 disposed on the linear module 4411, and a gripper 4413 disposed on the lifting module 4412. The gripper 4413 is used to grip the PCR plate. The gripper 4413 moves up and down under the drive of the lifting module 4412 and moves laterally under the drive of the linear module 4411.

[0049] To minimize the movement paths of the first transfer mechanism 431 and the second transfer mechanism 433, the portions of the inter-compartment transfer assembly 200 located within the sealing cavity 1021, the sealing device 410, and the inter-compartment transfer mechanism 432 located within the sealing cavity 1021 are all arranged on one side of the length direction of the linear module 4411 of the first transfer mechanism 431. The portions of the inter-compartment transfer mechanism 432 located within the detection cavity 1022 and the PCR detector 420 are both located on one side of the length direction of the linear module 4411 of the second transfer mechanism 433.

[0050] Specifically, the gripper 4413 includes two gripping plates and a drive mechanism for opening and closing the two gripping plates relative to each other. Grooves are designed on the inner sides of the two gripping plates where they contact the PCR plate, and wear-resistant pads are attached to increase resistance. It is conceivable that the gripper 4413 could also be an electric gripper or a pneumatic gripper.

[0051] The structure of the inter-cavity transfer mechanism 432 can be the same as that of the inter-cavity transfer assembly 200. In one embodiment, such as... Figures 3-4 As shown, the cross-compartment transfer assembly 200 includes a linear module 210 and a support 220 disposed on the linear module 210. The support 220 is used to support the PCR plate and moves along the length direction of the linear module 210 under the drive of the linear module 210.

[0052] The linear slide can be a synchronous belt type, a ball screw type, or a linear motor type. Of course, the linear module 210 can also be replaced by a cylinder or an electric cylinder.

[0053] In one embodiment, such as Figures 1-3As shown, the sample processing device 300 includes a cupping module 310, a nucleic acid extraction module 320, a robotic arm module 330, and a solution preparation module (not shown in the figure). The cupping module 310 is used to cup the samples, and the nucleic acid extraction module 320 is used to extract nucleic acids from the cupped samples. The robotic arm module 330 has a gripper 331 and a pipette 332 that can move within the sample processing chamber 101. The solution preparation module is used to cooperate with the pipette 332 to construct the PCR reaction system.

[0054] The sample processing device 300 also includes a consumable storage module 500, which is disposed in the sample processing chamber 101. The consumable storage module 500 is provided with partitioned placement seats for storing deep well plates, PCR plates and pipette tips. The robotic arm module 330 can also transfer consumables between the consumable storage module 500 and the dispensing cup module 310, between the consumable storage module 500 and the nucleic acid extraction module 320, and between the consumable storage module 500 and the solution preparation module.

[0055] The sample tube separation module 310 includes a tube rack 311, a barcode scanner 312, a clamping assembly 313, and a deep-well plate holder 314. The tube rack 311 is used to hold the sample tubes. The barcode scanner 312 is located on one side of the tube rack 311 and is used to input information about the sample tubes to achieve sample traceability and tracking. The clamping assembly 313 is used to clamp the sample tubes. The clamping assembly 313 can be a clamping mechanism that can be opened and closed, including but not limited to electric grippers and pneumatic grippers, as long as it can clamp and fix the tube body of the sample tube. The gripper 331 can transfer the sample tube from the tube rack 311 to the clamping assembly 313 and cooperate with the clamping assembly 313 to open the tube cap of the sample tube. The pipette 332 is used to transfer the sample liquid in the sample tube to the deep-well plate on the deep-well plate holder 314 for separation operation.

[0056] The nucleic acid extraction module 320 is based on magnetic bead nucleic acid extraction, including upper magnetic suction type or lower magnetic suction type, which is not limited here. In practice, depending on the sample processing volume, an 8-throughput nucleic acid extractor, a 16-throughput nucleic acid extractor, a 32-throughput nucleic acid extractor, a 64-throughput nucleic acid extractor, or a 96-channel nucleic acid extractor can be selected. The number can be set to multiple as needed. When one nucleic acid extraction module 320 is running, another nucleic acid extraction module 320 can be added at any time for extraction.

[0057] The solution preparation module includes a solution preparation base for supporting the PCR plate and a reagent tube storing the PCR reaction solution. The pipette 332 in the robotic arm module 330 transfers the extracted nucleic acid and PCR reaction solution to the PCR plate located on the solution preparation base to construct the PCR reaction system.

[0058] During operation, gripper 331 picks up the deep-well plate used for dispensing at consumable storage module 500 and transfers it to deep-well plate holder 314. Gripper 331 picks up the sample tube on tube holder 311 and transfers it to clamping assembly 313. Clamping assembly 313 actuates to clamp the sample tube body. Gripper 331 picks up the tube cap and rotates it to open the tube cap. At the same time, pipette 332 moves to consumable storage module 500 to press the pipette tip, then moves to clamping assembly 313 to aspirate the sample liquid from the sample tube and moves to deep-well plate for dispensing. After dispensing, gripper 331 picks up the deep-well plate containing the sample liquid and moves it to nucleic acid extraction module 320 for nucleic acid extraction. The nucleic acid extracted by nucleic acid extraction module 320 is transferred to liquid preparation module by pipette 332 and mixed with reagents in PCR plate to construct PCR reaction system.

[0059] In one specific embodiment, such as Figures 2-3 As shown, the robotic arm module 330 includes a first linear module, two second linear modules disposed on the first linear module, and a lifting module 4412 disposed on the second linear modules. The first linear module is located above the dispensing module 310, the nucleic acid extraction module 320, and the liquid preparation module. The lifting module 4412 can move along a first direction under the drive of the second linear modules connected to it, and move along a second direction under the drive of the first linear module. The first direction is perpendicular to the second direction. The gripper 331 and the pipette 332 are respectively disposed on different lifting modules 4412. The gripper 331 can move up and down under the drive of the lifting module 4412 connected to it, and the gripper 331 can also rotate relative to the lifting module 4412. The pipette 332 can move up and down under the drive of the lifting module 4412 connected to it.

[0060] Specifically, the gripper 331 can be a rotating gripper, and the distance between the two gripping arms of the gripper 331 is adjustable to grip objects of different widths, thereby enabling the gripping of consumables such as sample tubes, deep-well plates, and PCR plates. The pipette 332 can be a single-head pipette or a multi-head pipette, which is not limited here.

[0061] In some embodiments, the nucleic acid extraction and detection integrated machine also includes an air filtration system (not shown in the figure), which is installed on the housing 100 and used to filter the gas inside the sample processing chamber 101. In addition, the sample processing chamber 101 is equipped with an ultraviolet lamp sterilization function, which, together with the high-efficiency HEPA filtration system, avoids aerosol contamination and ensures the reliability of experimental results.

[0062] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0063] The above embodiments are merely illustrative of several implementation methods of this application, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this application should be determined by the appended claims.

Claims

1. A nucleic acid extraction and detection all-in-one machine, characterized in that, The application relates to a sample processing and amplification detection device. The device comprises a housing (100) provided with a sample processing chamber (101) and an amplification detection chamber (102) which are independent of each other and are communicated through a sample transmission channel (103); a cross-chamber transmission assembly (200) arranged through the sample transmission channel (103) and used for transmitting samples in the sample processing chamber (101) and between the amplification detection chamber (102); a sample processing device (300) arranged in the sample processing chamber (101) and used for cup separation, nucleic acid extraction and PCR reaction system construction; and a PCR detection device (400) arranged in the sample processing chamber (101) and used for PCR amplification detection. The PCR detection device (400) comprises a film sealing instrument (410), a PCR detection instrument (420) and a transfer device (430), the film sealing instrument (410) is used for sealing the PCR plate after the PCR system construction in the sample processing device (300), the PCR detection instrument (420) is used for PCR amplification and detection, and the transfer device (430) is used for transferring the PCR plate between the cross-chamber transmission assembly (200), the film sealing instrument (410) and the PCR detection instrument (420). The amplification detection chamber (102) is divided into a film sealing cavity (1021) and a detection cavity (1022) by a partition plate (1020), the partition plate (1020) is provided with a transmission port (104) for communicating the film sealing cavity (1021) and the detection cavity (1022), the film sealing instrument (410) is arranged in the film sealing cavity (1021), and the PCR detection instrument (420) is arranged in the detection cavity (1022). The transfer device (430) comprises a first transfer mechanism (431), an inter-cavity transfer mechanism (432) and a second transfer mechanism (433), the inter-cavity transfer mechanism (432) is arranged through the transmission port (104), the first transfer mechanism (431) is arranged in the film sealing cavity (1021) and is used for transferring the PCR plate between the cross-chamber transmission assembly (200), the film sealing instrument (410) and the inter-cavity transfer mechanism (432), and the second transfer mechanism (433) is arranged in the detection cavity (1022) and is used for transferring the PCR plate between the inter-cavity transfer mechanism (432) and the film sealing instrument (410).

2. The nucleic acid extraction and detection all-in-one machine according to claim 1, characterized in that, The first transfer mechanism (431) and the second transfer mechanism (433) are the same in structure and each comprises a linear module (4411), a lifting module (4412) arranged on the linear module (4411) and a clamping jaw (4413) arranged on the lifting module (4412), the clamping jaw (4413) is used for grabbing the PCR plate, the clamping jaw (4413) moves up and down under the drive of the lifting module (4412) and moves laterally under the drive of the linear module (4411). 3.The nucleic acid extraction and detection all-in-one machine according to claim 2, characterized in that, ​ ​ 4. The nucleic acid extraction and detection all-in-one machine according to claim 3, characterized in that, ​ 5. The nucleic acid extraction and detection all-in-one machine according to claim 1, characterized in that, The cross-warehouse transmission assembly (200) comprises a linear module (210) and a bearing seat (220) arranged on the linear module (210), and the bearing seat (220) is used for bearing a PCR plate. 6.The nucleic acid extraction and detection all-in-one machine according to claim 1, characterized in that, The sample processing device (300) comprises a cup dividing module (310), a nucleic acid extraction module (320), a mechanical arm module (330) and a liquid preparation module, the cup dividing module (310) is used for cup dividing of samples, and the nucleic acid extraction module (320) is used for nucleic acid extraction of the cup-divided samples; the mechanical arm module (330) has a gripper (331) and a pipette gun (332) capable of moving in the sample processing warehouse (101), and the liquid preparation module is used for cooperating with the pipette gun (332) to construct a PCR reaction system.

7. The nucleic acid extraction and detection all-in-one machine according to claim 6, characterized in that, The cup dividing module (310) comprises a tube rack (311), a code scanner (312), a clamping assembly (313) and a deep-well plate rack (314), the tube rack (311) is used for bearing sample tubes, and the code scanner (312) is used for information input of the sample tubes; the clamping assembly (313) is used for clamping the sample tubes, the gripper (331) can transfer the sample tubes from the tube rack (311) to the clamping assembly (313), and cooperate with the clamping assembly (313) to open the tube cover of the sample tubes, and the pipette gun (332) is used for transferring the samples in the sample tubes to the deep-well plate on the deep-well plate rack (314) to perform cup dividing operation. 8.The nucleic acid extraction and detection all-in-one machine according to claim 6, characterized in that, The nucleic acid extraction module (320) comprises an 8-plex nucleic acid extractor, a 16-plex nucleic acid extractor, a 32-plex nucleic acid extractor, a 64-plex nucleic acid extractor or a 96-channel nucleic acid extractor. 9.The nucleic acid extraction and detection all-in-one machine according to claim 6, characterized in that, The sample processing device (300) further comprises a consumable storage module (500), the consumable storage module (500) is arranged in the sample processing warehouse (101), and the consumable storage module (500) is provided with a partitioned placement seat for storing deep-well plates, PCR plates and pipette tips, and the mechanical arm module (330) can further transfer consumables between the consumable storage module (500) and the cup dividing module (310), between the consumable storage module (500) and the nucleic acid extraction module (320), and between the consumable storage module (500) and the liquid preparation module.

10. The nucleic acid extraction and detection all-in-one machine according to any one of claims 1-9, characterized in that, The nucleic acid extraction and detection all-in-one machine further comprises an air filtration system arranged on the machine shell (100) and used for filtering the gas inside the sample processing warehouse (101).

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