Multifunctional embryo culture dish for reproductive medicine

Abstract

The utility model provides a multifunctional embryo culture dish for reproductive medicine, which belongs to the field of reproductive medicine and comprises a dish bottom, a central well is arranged at the top of the dish bottom, a waste liquid well is arranged on one side of the central well, a culture hole is arranged on one side of the waste liquid well, a placing groove is arranged in the dish bottom and is positioned right below the culture hole, and the culture hole is arranged in the placing groove. And a temperature control base is arranged in the placement groove. Through the arrangement of the central well, the waste liquid well, the culture holes, the placing groove and the temperature control base, the waste liquid well, the culture holes and the temperature control base can be additionally designed under the condition that the central well is reserved, the waste liquid well can be used for storing waste liquid, and the culture holes are multiple, so that more than two embryos can be cultured; various experimental data can be conveniently compared, the temperature control base can keep the temperature in the culture hole, the temperature is constant at about 37 DEG C, and the survival rate of embryos is increased.

Description

Technical Field

[0001] This utility model relates to the field of reproductive medicine, and more specifically, to a multifunctional embryo culture dish for reproductive medicine. Background Technology

[0002] Reproductive medicine is a discipline that focuses on the regulation of male and female reproductive functions, basic and clinical issues related to human reproductive health, infertility, and assisted reproductive technologies. It aims to reveal the mysteries of reproduction and solve human fertility problems. Embryo culture dishes are required when conducting reproductive medicine experiments.

[0003] A search revealed that Chinese patent CN218666099U discloses "a multifunctional culture dish for assisted reproduction, which retains the central well shape at the bottom of a cylindrical dish, but redesigns the surrounding concentric arc-shaped groove area, adding four conical culture holes, and retaining a waste liquid well in part of the concentric arc-shaped groove area for waste liquid discharge. It can culture two or more embryos together and can realize more functions such as gamete fertilization, embryo transfer, embryo washing, embryo culture, egg and embryo freezing, egg and embryo thawing, and embryo culture, saving reagent costs, quality control costs, and laboratory management costs. The dish lid is provided with four supporting ribs in conjunction with the supporting convex edge at the bottom to support the lid. Four ventilation grooves are evenly distributed around the perimeter of the lid to provide a breathing environment for embryo culture. The supporting convex edge is provided with a wavy protrusion to facilitate the handling of the culture dish." However, it still has the following defects:

[0004] Although the device can culture embryos, it lacks a temperature control system. Inappropriate temperatures may damage embryonic cells, thereby affecting the survival rate and quality of the embryos.

[0005] Therefore, we have made improvements to this and proposed a multifunctional embryo culture dish for reproductive medicine. Utility Model Content

[0006] The purpose of this invention is to address the problem that while existing multifunctional embryo culture dishes for reproductive medicine can culture embryos, they lack a constant temperature device. Inappropriate temperatures may damage embryo cells, thereby affecting the survival rate and quality of the embryos.

[0007] To achieve the above-mentioned objectives, this utility model provides the following technical solution:

[0008] Multifunctional embryo culture dishes for reproductive medicine aim to improve the above-mentioned problems.

[0009] The specific details of this utility model are as follows:

[0010] The device includes a dish bottom, a central well at the top of the dish bottom, a waste liquid well on one side of the central well, a culture well on one side of the waste liquid well, a placement groove inside the dish bottom and directly below the culture well, and a temperature control base inside the placement groove.

[0011] As a preferred technical solution of this utility model, a lid is provided on one side of the bottom of the dish, and a trapezoidal block is fixedly connected to the inner bottom wall of the lid. The trapezoidal block is five in number and is distributed circumferentially along the central axis of the lid.

[0012] As a preferred technical solution of this utility model, an air inlet is provided on the outer wall of the lid, and a HEPA filter is fixedly connected inside the air inlet. There are five air inlets and five HEPA filters, which are distributed circumferentially along the central axis of the lid.

[0013] As a preferred technical solution of this utility model, the outer wall of the bottom of the dish is provided with anti-slip protrusions, and the number of anti-slip protrusions is six and they are distributed circumferentially along the central axis of the bottom of the dish.

[0014] As a preferred technical solution of this utility model, an identification frame is fixedly connected to the outer wall of the bottom of the dish, and the identification frame is located between two adjacent anti-slip protrusions.

[0015] As a preferred technical solution of this utility model, six anti-slip pads are fixedly connected to the outer bottom wall of the dish, and the material of the six anti-slip pads is food-grade silicone.

[0016] As a preferred technical solution of this utility model, both the interior of the central well and the culture well are provided with a polypeptide coating.

[0017] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0018] In the solution of this utility model:

[0019] By incorporating a central well, waste liquid well, culture wells, placement tanks, and a temperature-controlled base, additional designs can be added while retaining the central well. The waste liquid wells can store waste liquids, and the multiple culture wells allow for the culture of more than two embryos, facilitating the comparison of various experimental data. The temperature-controlled base maintains the internal temperature of the culture wells at a constant level of around 37°C, improving embryo survival rates. Attached Figure Description

[0020] Figure 1 A schematic diagram of the structure of the multifunctional embryo culture dish for reproductive medicine provided by this utility model;

[0021] Figure 2A front view of the multifunctional embryo culture dish for reproductive medicine provided by this utility model;

[0022] Figure 3 The multifunctional embryo culture dish for reproductive medicine provided by this utility model Figure 2 A three-dimensional cross-sectional view at point AA;

[0023] Figure 4 The lower view of the multifunctional embryo culture dish for reproductive medicine provided by this utility model;

[0024] Figure 5 The multifunctional embryo culture dish for reproductive medicine provided by this utility model Figure 3 Enlarged view of point A in the middle.

[0025] The image shows:

[0026] 1. Dish bottom; 2. Central well; 3. Waste liquid well; 4. Culture well; 5. Placement tank; 6. Temperature control base; 7. Dish lid; 8. Trapezoidal block; 9. Air inlet; 10. HEPA filter; 11. Anti-slip protrusions; 12. Identification frame; 13. Anti-slip mat; 14. Peptide coating. Detailed Implementation

[0027] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model.

[0028] Therefore, the following detailed description of the embodiments of this utility model is not intended to limit the scope of the claimed utility model, but merely to illustrate some embodiments of the utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without inventive effort are within the scope of protection of this utility model.

[0029] It should be noted that, unless otherwise specified, the embodiments and features and technical solutions in the present invention can be combined with each other.

[0030] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0031] like Figure 1-5As shown, this embodiment proposes a multifunctional embryo culture dish for reproductive medicine, including a dish bottom 1, a central well 2 at the top of the dish bottom 1, a waste liquid well 3 on one side of the central well 2, a culture well 4 on one side of the waste liquid well 3, a placement groove 5 inside the dish bottom 1 located directly below the culture well 4, and a temperature control base 6 inside the placement groove 5. The central well 2 is located at the center of the top of the dish bottom 1. There are two waste liquid wells 3 arranged opposite each other, and four culture wells 4 arranged opposite each other in pairs. There are two placement grooves 5, and the length of the placement groove 5 is greater than the distance between two adjacent culture wells 4. The placement groove 5 is not connected to the interior of the waste liquid well 3. The temperature control base 6 is connected to an external power source. With the central well 2 retained, an additional design is made. During the experiment, the embryo is placed inside the culture well 4 for culture, and then the temperature control base 6 is activated to heat the culture well 4, so that the temperature inside the culture well 4 is about 37°C, thereby improving the embryo survival rate. The waste liquid well 3 can store waste liquid for convenient subsequent unified treatment.

[0032] like Figure 1 As shown, a lid 7 is provided on one side of the dish bottom 1. Five trapezoidal blocks 8 are fixedly connected to the inner bottom wall of the lid 7 and are distributed circumferentially along the central axis of the lid 7. The inner diameter of the lid 7 is slightly larger than the outer diameter of the dish bottom 1. When in use, the lid 7 can play a role in heat preservation and moisture retention, which helps to maintain the temperature and humidity in the culture well 4 and provide a more stable growth environment for the embryo. The trapezoidal blocks 8 can make a certain distance between the top of the dish bottom 1 and the lid 7 to ensure gas circulation.

[0033] like Figure 1 As shown, an air inlet 9 is provided on the outer wall of the dish lid 7. A HEPA filter 10 is fixedly connected inside the air inlet 9. There are five air inlets 9 and five HEPA filters 10, which are distributed circumferentially along the central axis of the dish lid 7. When in use, the air inlet 9 and the trapezoidal block 8 work together to improve the air circulation efficiency. At the same time, the HEPA filter 10 can prevent airborne particles from entering the culture well 4, thereby improving the success rate of the experiment.

[0034] like Figure 1 As shown, the outer wall of the dish bottom 1 is provided with anti-slip protrusions 11. There are six anti-slip protrusions 11, which are distributed circumferentially along the central axis of the dish bottom 1. When in use, the dish bottom 1 can be easily picked up by the anti-slip protrusions 11 to avoid slipping.

[0035] like Figure 1 As shown, an identification frame 12 is fixedly connected to the outer wall of the bottom of the dish 1. The identification frame 12 is located between two adjacent anti-slip protrusions 11. When in use, a paper with embryo data can be placed inside the identification frame 12 so that others can easily understand the embryo data.

[0036] like Figure 4As shown, six anti-slip pads 13 are fixedly connected to the outer bottom wall of the dish bottom 1. The six anti-slip pads 13 are all made of food-grade silicone. When in use, the anti-slip pads 13 made of food-grade silicone are safe and non-toxic, soft and have good anti-slip performance, preventing the dish bottom 1 from slipping on the external laboratory table.

[0037] like Figure 3 As shown, both the central well 2 and the culture well 4 are provided with a polypeptide coating 14. When in use, the polypeptide coating 14 can promote the attachment and growth of embryos on the culture dish.

[0038] Specifically, when using this multifunctional embryo culture dish for reproductive medicine: place the embryo inside the culture well 4 for culture, then activate the temperature control base 6 to heat the culture well 4, maintaining the internal temperature at approximately 37°C to improve embryo survival rate. The waste liquid well 3 can store waste liquid for convenient subsequent unified treatment. Then, lift the dish lid 7 so that the trapezoidal block 8 fits against the top of the dish bottom 1, which helps maintain the temperature and humidity inside the culture well 4, providing a more stable growth environment for the embryo. The air inlet 9, used in conjunction with the trapezoidal block 8, can improve air circulation efficiency, while the HEPA filter 10 can prevent airborne particles from entering the culture well 4, improving the success rate of the experiment.

[0039] All technical features in this embodiment can be freely combined according to actual needs.

[0040] The above embodiments are preferred implementations of this utility model. In addition, this utility model can also be implemented in other ways. Any obvious substitutions without departing from the concept of this technical solution are within the protection scope of this utility model.

Claims

1. A multi-functional embryo culture dish for reproductive medicine comprising a dish bottom (1), characterized in that, The top of the dish bottom (1) is provided with a central well (2), one side of the central well (2) is provided with a waste well (3), one side of the waste well (3) is provided with a culture hole (4), the inside of the dish bottom (1) is provided with a placing groove (5), which is located directly below the culture hole (4), and the inside of the placing groove (5) is provided with a temperature control base (6).

2. The multi-functional embryo culture dish for reproductive medicine according to claim 1, wherein One side of the dish bottom (1) is provided with a dish cover (7), the inner bottom wall of the dish cover (7) is fixedly connected with a trapezoidal block (8), the number of the trapezoidal block (8) is five and is distributed along the central axis of the dish cover (7).

3. The multi-functional embryo culture dish for reproductive medicine according to claim 2, wherein The outer wall of the dish cover (7) is provided with an air inlet hole (9), the inside of the air inlet hole (9) is fixedly connected with a HEPA filter screen (10), the number of the air inlet hole (9) and the HEPA filter screen (10) is five and is distributed along the central axis of the dish cover (7).

4. The multi-functional embryo culture dish for reproductive medicine according to claim 1, wherein The outer wall of the dish bottom (1) is provided with an anti-skid protrusion (11), the number of the anti-skid protrusion (11) is six and is distributed along the central axis of the dish bottom (1).

5. The multi-functional embryo culture dish for reproductive medicine according to claim 4, wherein The outer wall of the dish bottom (1) is fixedly connected with an identification frame (12), the identification frame (12) is located between two adjacent anti-skid protrusions (11).

6. The multi-purpose embryo culture dish for reproductive medicine according to claim 1, wherein The outer bottom wall of the dish bottom (1) is fixedly connected with six anti-skid pads (13), the material of the six anti-skid pads (13) is food-grade silica gel.

7. The multi-functional embryo culture dish for reproductive medicine according to claim 1, wherein The inside of the central well (2) and the culture hole (4) is provided with a polypeptide coating (14).

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