Experimental reagent centrifugal separation device
By designing a centrifugal separation device for experimental reagents with a rotating rack and a liquid delivery tank, the problem of cells being carried away by traditional pipettes was solved, thus ensuring the accuracy and reliability of experimental results and protecting the safety of experimental personnel and the environment.
Patent Information
- Application Number
- CN202520042752.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-09
- Publication Date
- 2026-01-02
- Estimated Expiration
- 2035-01-09
AI Technical Summary
Traditional pipettes can easily remove cells when removing liquid, leading to differences in cell count between experimental groups and affecting the accuracy and reliability of experimental results.
Design an experimental reagent centrifugation device that uses a rotating frame to rotate the well plate and generate centrifugal force to prevent cells from being carried away when removing liquid. The design of the rotating frame and liquid guide channel with a 60-degree cross arrangement ensures that the liquid does not flow out when it is stationary and detaches along the well wall during rotation, thus avoiding cross-contamination.
To ensure the accuracy and reliability of experimental results, avoid environmental pollution from waste liquid, protect the health of laboratory personnel, and reduce the risk of cross-contamination.
Smart Images
Figure CN223747768U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to experimental equipment technical field, concretely relates to a reagent centrifugal separation device for experiment. BACKGROUND
[0002] CCK-8 experiment, that is, cell counting kit-8 (Cell Counting Kit-8) experiment. When performing CCK-8 experiment, one of the key steps is to remove the liquid in the 96-well plate. The traditional operation method is to use a liquid suction device, insert the gun head into the well plate and extend to the bottom of the well plate to suck the waste liquid. However, this method has obvious disadvantages. Since the gun head is in close contact with the bottom cells during the suction process, it is easy to suck a part of the cells, thereby causing the number of cells in each experimental group to differ, affecting the accuracy and reliability of the experimental results.
[0003] Therefore, the above problems need to be solved. UTILITY MODEL CONTENT
[0004] The utility model aims at overcoming the above shortcomings, and provides a reagent centrifugal separation device for experiment, which can remove the liquid while avoiding taking away the cells, avoiding the difference in the number of cells between experimental groups, and ensuring the accuracy and reliability of the experimental structure.
[0005] Technical solution: In order to achieve the above-mentioned purpose, the utility model provides an experimental reagent centrifugal separation device, including the casing, being equipped with the separation cavity in the casing, the separation cavity upper end opening is equipped with the apron, the apron one side is hinged to the casing top surface one side. The separation cavity bottom is equipped with the rotating shaft and the rotating frame, the rotating shaft is vertically arranged, and the rotating shaft and the driving motor driving connection equipped with in the casing, and the rotating shaft and the rotating frame are connected. The rotating frame includes the first edge, the second edge and the bottom. The first edge, the second edge and the bottom are sequentially connected to form a triangle, the bottom is horizontally arranged, and the first edge and the second edge are arranged on the upper side of the bottom. The first edge and the second edge are respectively connected with the fixing device, and the fixing device is clamped with the hole plate. In the utility model, the hole plate is preferably a 96-hole plate, and the hole bottom of the 96-hole plate is flat to adapt to cell adhesion and growth, and facilitate observation of cell state. In CCK experiment (Cell Counting Kit experiment), cells are inoculated in the hole plate, and after culture, cells adhere and spread along the bottom of the hole. When pipetting is needed, the hole plate is clamped on the fixing device, the driving motor in the casing is started, the driving motor drives the rotating frame to rotate through the rotating shaft, and then drives the hole plate clamped on the fixing device to rotate. The rotating hole plate generates centrifugal force due to rotation. When the centrifugal force generated is sufficient to overcome the gravity of the liquid, the liquid in the hole of the hole plate is affected by the centrifugal force and flies out of the hole. The cells inoculated in the hole of the hole plate adhere to the bottom of the hole due to the adhesion and spreading along the bottom of the hole. The centrifugal force is insufficient to overcome the gravity and adhesion of the cells, and the cells still adhere to the bottom of the hole of the hole plate. The rotating frame is continuously rotated until the liquid in the hole of the hole plate is completely removed, ensuring the pipetting effect and avoiding the problem that part of the cells are removed when the traditional pipette removes the liquid, ensuring the accuracy and reliability of the experimental results. The whole pipetting process is carried out in the separation cavity, which can avoid the pollution of waste liquid to the surrounding environment and protect the health and safety of the experimental personnel. The utility model is also applicable to the repeated washing process of ELISA experiment. After the hole is wetted, the washing liquid in the hole plate is discharged from the hole, reducing the experimental personnel's beating of the hole plate and avoiding cross contamination caused by splashing of the washing liquid due to beating.
[0006] Further, in the experimental reagent centrifugal separation device described above, the first edge and the second edge intersect at 60 degrees. The first edge and the second edge are arranged at 60 degrees. At this time, the hole plate and the vertical direction are arranged at an angle of 30 degrees, ensuring that the liquid in the hole of the hole plate does not flow out in a static state, and the liquid in the hole of the hole plate can be separated along the hole wall during rotation. In order to ensure that the liquid in the hole of the hole plate can overcome gravity, the rotating speed of the rotating frame is preferably 50-120 rpm.
[0007] Further, the experimental reagent centrifugal separation device, the bottom side is connected with a long plate, the top surface of the long plate is connected with a shaft sleeve, and the shaft sleeve and the rotating shaft are coaxially and synchronously connected. The long plate is connected through the shaft sleeve and the rotating shaft, the shaft sleeve is sleeved on the outside of the rotating shaft, and the shaft sleeve and the rotating shaft are connected through a pin, so that the shaft sleeve and the rotating shaft are synchronously connected, then the rotating frame is connected to the top surface of the long plate, the whole structure is simple, and the rotating frame can be completely disassembled for cleaning and maintenance.
[0008] Further, the experimental reagent centrifugal separation device, the fixing device includes a first side baffle, a second side baffle, a bottom plate and a lower baffle, the bottom plate is a square plate with a long side extending downward, the first side baffle and the second side baffle are connected to the upper side of the bottom plate, the first side baffle and the second side baffle are arranged along the long side of the bottom plate, the hole plate is clamped between the first side baffle and the second side baffle, and the lower baffle is arranged along the lower side of the bottom plate. The hole plate is clamped on both sides by the first side baffle and the second side baffle, and the lower side of the hole plate is limited by the lower baffle, so as to ensure the installation position of the hole plate, ensure that the hole plate rotates in the same position, ensure that the liquid in the hole of the hole plate is subjected to a centrifugal force sufficient to overcome gravity, and ensure the effect of pipetting.
[0009] Further, the experimental reagent centrifugal separation device, the first side baffle and the second side baffle are provided with clamping grooves on the opposite sides, and the hole plate is clamped in the clamping grooves on both sides. The hole plate is clamped in the clamping grooves to avoid displacement of the hole plate during rotation, and the clamping method in the clamping grooves is accurate in positioning and convenient to install. When the hole plate needs to be removed, the hole plate can be pulled out from the upper end of the clamping groove, which is convenient to disassemble and reduces vibration of the hole plate during disassembly.
[0010] Further, the experimental reagent centrifugal separation device, the first side baffle is provided with a first inclined surface at one end away from the bottom plate, and the first inclined surface is inclined to the direction of the clamping groove provided on the first side baffle. The second side baffle is provided with a second inclined surface at one end away from the bottom plate, and the second inclined surface is inclined to the direction of the clamping groove provided on the second side baffle. When the hole plate needs to be clamped in the clamping groove, one side of the hole plate should be clamped in the clamping groove first, the other side of the hole plate is placed on the first inclined surface or the second inclined surface, then the hole plate is pressed, the hole plate moves along the first inclined surface or the second inclined surface, the hole plate deforms slightly, until the hole plate is clamped in the clamping groove, and the hole plate returns to its original state. The first inclined surface and the second inclined surface can guide the clamping of the hole plate in the clamping groove, which is convenient to install and can reduce the vibration of the hole plate during installation, avoid splashing of the liquid in the hole plate, avoid cross contamination between samples, and ensure the accuracy and reliability of the experiment.
[0011] Further, the experimental reagent centrifugal separation device has the beneficial effects that: the experimental reagent centrifugal separation device of the utility model, through continuously rotating the rotating frame, liquid in the hole of the hole plate is removed completely, the problem that part of cells is removed when a traditional pipette removes liquid is avoided, and the accuracy and reliability of experimental results are ensured. The whole pipetting process is carried out in the separation cavity, waste liquid does not pollute the surrounding environment, and the health and safety of experimental personnel are protected. The first edge and the second edge are intersected, liquid in the hole of the hole plate does not flow out in a static state, and liquid in the hole of the hole plate can be separated from the hole wall in a rotating process. When liquid is separated from the hole plate, liquid flows to the liquid collecting groove along the inner wall of the separation cavity, and is discharged from the liquid outlet hole into the liquid collecting box, so that waste liquid can be collected, and the environment is prevented from being polluted by waste liquid. The liquid guide groove is arranged, liquid is prevented from entering other holes, pollution of other samples is avoided, the accuracy and reliability of the experiment are ensured.
[0012] Further, the experimental reagent centrifugal separation device has the beneficial effects that: the experimental reagent centrifugal separation device of the utility model, through continuously rotating the rotating frame, liquid in the hole of the hole plate is removed completely, the problem that part of cells is removed when a traditional pipette removes liquid is avoided, and the accuracy and reliability of experimental results are ensured. The whole pipetting process is carried out in the separation cavity, waste liquid does not pollute the surrounding environment, and the health and safety of experimental personnel are protected. The first edge and the second edge are intersected, liquid in the hole of the hole plate does not flow out in a static state, and liquid in the hole of the hole plate can be separated from the hole wall in a rotating process. When liquid is separated from the hole plate, liquid flows to the liquid collecting groove along the inner wall of the separation cavity, and is discharged from the liquid outlet hole into the liquid collecting box, so that waste liquid can be collected, and the environment is prevented from being polluted by waste liquid. The liquid guide groove is arranged, liquid is prevented from entering other holes, pollution of other samples is avoided, the accuracy and reliability of the experiment are ensured.
[0013] Further, the experimental reagent centrifugal separation device has the beneficial effects that: the experimental reagent centrifugal separation device of the utility model, through continuously rotating the rotating frame, liquid in the hole of the hole plate is removed completely, the problem that part of cells is removed when a traditional pipette removes liquid is avoided, and the accuracy and reliability of experimental results are ensured. The whole pipetting process is carried out in the separation cavity, waste liquid does not pollute the surrounding environment, and the health and safety of experimental personnel are protected. The first edge and the second edge are intersected, liquid in the hole of the hole plate does not flow out in a static state, and liquid in the hole of the hole plate can be separated from the hole wall in a rotating process. When liquid is separated from the hole plate, liquid flows to the liquid collecting groove along the inner wall of the separation cavity, and is discharged from the liquid outlet hole into the liquid collecting box, so that waste liquid can be collected, and the environment is prevented from being polluted by waste liquid. The liquid guide groove is arranged, liquid is prevented from entering other holes, pollution of other samples is avoided, the accuracy and reliability of the experiment are ensured.
[0014] Further, the experimental reagent centrifugal separation device has the beneficial effects that: the experimental reagent centrifugal separation device of the utility model, through continuously rotating the rotating frame, liquid in the hole of the hole plate is removed completely, the problem that part of cells is removed when a traditional pipette removes liquid is avoided, and the accuracy and reliability of experimental results are ensured. The whole pipetting process is carried out in the separation cavity, waste liquid does not pollute the surrounding environment, and the health and safety of experimental personnel are protected. The first edge and the second edge are intersected, liquid in the hole of the hole plate does not flow out in a static state, and liquid in the hole of the hole plate can be separated from the hole wall in a rotating process. When liquid is separated from the hole plate, liquid flows to the liquid collecting groove along the inner wall of the separation cavity, and is discharged from the liquid outlet hole into the liquid collecting box, so that waste liquid can be collected, and the environment is prevented from being polluted by waste liquid. The liquid guide groove is arranged, liquid is prevented from entering other holes, pollution of other samples is avoided, the accuracy and reliability of the experiment are ensured.
[0015] The above technical scheme can be seen, the utility model has the beneficial effects that: the experimental reagent centrifugal separation device of the utility model, through continuously rotating the rotating frame, liquid in the hole of the hole plate is removed completely, the problem that part of cells is removed when a traditional pipette removes liquid is avoided, and the accuracy and reliability of experimental results are ensured. The whole pipetting process is carried out in the separation cavity, waste liquid does not pollute the surrounding environment, and the health and safety of experimental personnel are protected. The first edge and the second edge are intersected, liquid in the hole of the hole plate does not flow out in a static state, and liquid in the hole of the hole plate can be separated from the hole wall in a rotating process. When liquid is separated from the hole plate, liquid flows to the liquid collecting groove along the inner wall of the separation cavity, and is discharged from the liquid outlet hole into the liquid collecting box, so that waste liquid can be collected, and the environment is prevented from being polluted by waste liquid. The liquid guide groove is arranged, liquid is prevented from entering other holes, pollution of other samples is avoided, the accuracy and reliability of the experiment are ensured. BRIEF DESCRIPTION OF DRAWINGS
[0016] Figure 1 It is a structure diagram of the experimental reagent centrifugal separation device of the utility model;
[0017] Figure 2 This is a front view of the rotating frame;
[0018] Figure 3 This is a schematic diagram of the rotating frame structure;
[0019] Figure 4 for Figure 3 A magnified view of a portion of the image;
[0020] Figure 5 This is a schematic diagram of the interior of the separation chamber;
[0021] Figure 6 This is a cross-sectional view of the separation cavity;
[0022] Figure 7 This is a top view of the orifice plate.
[0023] In the diagram: 1. Shell, 11. Separation chamber, 111. Liquid collection tank, 112. Drain hole, 2. Cover plate, 12. Rotating shaft, 13. Liquid collection box, 14. Liquid baffle, 3. Rotating frame, 31. First side, 32. Second side, 33. Bottom edge, 34. Bushing, 35. Long plate, 4. Fixing device, 41. First side baffle, 410. Slot, 411. First inclined surface, 42. Second side baffle, 421. Second inclined surface, 43. Bottom plate, 44. Lower baffle, 5. Perforated plate, 51. Liquid guide tank. Detailed Implementation
[0024] Example 1
[0025] like Figures 1-2 The apparatus shown is a reagent centrifugation device for experiments, comprising a housing 1, a separation chamber 11 inside the housing 1, and a cover plate 2 at the upper opening of the separation chamber 11, one side of which is hinged to the top surface of the housing 1. A rotating shaft 12 and a rotating frame 3 are located at the bottom of the separation chamber 11. The rotating shaft 12 is vertically arranged and driven by a drive motor located inside the housing 1. The rotating shaft 12 is also connected to the rotating frame 3. The rotating frame 3 includes a first side 31, a second side 32, and a bottom side 33. The first side 31, the second side 32, and the bottom side 33 are connected end-to-end to form a triangle, with the bottom side 33 horizontally arranged. The first side 31 and the second side 32 are located above the bottom side 33. Fixing devices 4 are respectively connected to the first side 31 and the second side 32, and the fixing devices 4 are engaged with perforated plates 5. The first side 31 and the second side 32 intersect at a 60-degree angle. A long plate 35 is connected to the lower side of the bottom edge 33, and a bushing 34 is connected to the top surface of the long plate 35. The bushing 34 and the rotating shaft 12 are coaxially and synchronously connected.
[0026] like Figure 3The experimental reagent centrifugation apparatus shown includes a fixing device 4 comprising a first side baffle 41, a second side baffle 42, a base plate 43, and a lower baffle 44. The base plate 43 is a square plate with its long side extending downwards. The first side baffle 41, the second side baffle 42, and the lower baffle 44 are connected to the upper side of the base plate 43. The first side baffle 41 and the second side baffle 42 are arranged along the long side of the base plate 43. The perforated plate 5 is engaged between the first side baffle 41 and the second side baffle 42. The lower baffle 44 is arranged along the lower short side of the base plate 43. The two sides of the perforated plate 5 are engaged by the first side baffle 41 and the second side baffle 42, and the lower side of the perforated plate 5 is limited by the lower baffle 44.
[0027] like Figure 4 The experimental reagent centrifuge apparatus shown has slots 410 on opposite sides of the first side baffle 41 and the second side baffle 42, with the perforated plate 5 respectively engaging in the slots 410 on both sides. The first side baffle 41 has a first inclined surface 411 at its end away from the bottom plate 43, which is angled towards the slots 410 on the first side baffle 41. The second side baffle 42 has a second inclined surface 421 at its end away from the bottom plate 43, which is angled towards the slots 410 on the second side baffle 42.
[0028] The orifice plate 5 is snapped into the slot 410 to prevent displacement during rotation. This snap-fit installation method ensures accurate positioning and convenient installation. When the orifice plate 5 needs to be removed, it can be pulled out from the top of the slot 410, facilitating disassembly and reducing vibration.
[0029] like Figures 5-6 The experimental reagent centrifuge apparatus shown has a collection tank 111 on the bottom surface of the separation chamber 11. The collection tank 111 has an arc-shaped cross-section, and the bottom of the separation chamber 11 is inclined towards the collection tank 111. Multiple drainage holes 112 are provided at the lowest point of the collection tank 111. A collection box 13 (see...) is located on the lower side of the separation chamber 11. Figure 1 The liquid collection box 13 and the drain hole 112 are connected. The separation chamber 11 has a liquid-blocking part 14 at its center, which is arranged around the rotating shaft 12.
[0030] The utility model discloses a hole plate 5 preferably is 96 hole plate, and the hole bottom shape that hole plate 5 is equipped with is flat bottom.In CCK-8 experiment, cell is inoculated in hole plate 5, after cultivation, cell adheres and spreads along the bottom of hole.When needing pipetting, first, one side of hole plate 5 is clamped in the clamping groove 410, and the other side of hole plate 5 is placed in the first slope 411 or the second slope 421, then press hole plate 5 and place in the one side of first slope 411 or second slope 421, hole plate 5 moves along the first slope 411 or second slope 421, and hole plate 5 slightly deforms, until hole plate 5 is clamped into the clamping groove 410, and hole plate 5 restores original shape.Start the drive motor that is equipped in the shell 1, and the drive motor rotation speed accelerates to 120rpm.The drive motor rotates through the rotating shaft 12 and drives the rotating frame 3 to rotate, and then drive the hole plate 5 clamped in the fixing device 4 to rotate, and the rotating hole plate 5 generates centrifugal force due to rotation, when the centrifugal force generated is enough to overcome the gravity of liquid, the liquid in the hole of hole plate 5 is separated from the hole under the action of centrifugal force, and the cell inoculated in the hole of hole plate 5 adheres and spreads along the bottom of hole, and the centrifugal force is not enough to overcome the gravity and adhesion of cell, and the cell still adheres to the bottom of hole of hole plate 5, and the single rotation lasts 10s, and is repeated, until the liquid in the hole of hole plate 5 is removed completely.When the liquid is separated from hole plate 5, the liquid is thrown into the separation cavity 11 and flows to the liquid collecting groove 111 along the inner wall of separation cavity 11, and is discharged into the liquid collecting box 13 through the multiple liquid discharge holes 112 arranged at the lowest part of liquid collecting groove 111.The liquid collecting box 13 is pulled out, and the waste liquid is poured into the waste liquid collecting container and is uniformly treated.The pipetting is completed, and the hole plate 5 is pulled out from the upper end of clamping groove 410.
[0031] Example 2
[0032] The difference between the embodiment and example 1 is that, as shown in the experimental reagent centrifugal separation device, the top surface of hole plate 5 is provided with a liquid guide groove 51, the liquid guide groove 51 is arranged in a cross shape along the long and short edges of hole plate 5, the liquid guide groove 51 guides the flow direction of liquid, avoids the liquid from entering other holes of hole plate 5, and avoids cross contamination to other samples. Figure 7
[0033] The utility model is also applicable to the repeated washing link of ELISA experiment, and the washing liquid in the hole plate is discharged from the hole after the hole is wetted, the hole plate is beaten by the experimenter, cross contamination caused by splashing of the washing liquid due to beating is avoided.
[0034] The above examples are exemplary, and the purpose is to illustrate the technical concept and characteristics of the utility model, so that the person skilled in the art can understand the content of the utility model and implement it, and the protection scope of the utility model cannot be limited accordingly. Any equivalent change or modification made according to the spirit and essence of the utility model should be covered within the protection scope of the utility model.
Claims
1. A reagent centrifugal separation device for laboratory use, characterized by: The utility model provides a kind of separating device, including shell (1), the separating cavity (11) is equipped in the shell (1), the separating cavity (11) upper end opening is equipped with cover plate (2), and the cover plate (2) one side is hinged to the top surface one side of shell (1);The bottom of the separating cavity (11) is equipped with rotating shaft (12) and rotating frame (3), and the rotating shaft (12) is vertically arranged;The rotating shaft (12) and the drive motor driving connection that the shell (1) is equipped with are connected;The rotating frame (3) includes first edge (31), second edge (32) and bottom edge (33);The first edge (31), second edge (32) and bottom edge (33) are sequentially connected;The bottom edge (33) is horizontally arranged, and the first edge (31) and the second edge (32) are arranged on the upper side of the bottom edge (33);The first edge (31) and the second edge (32) are respectively connected with fixing device (4), and the fixing device (4) is clamped with hole plate (5).
2. The reagent centrifugal separation device for experiments according to claim 1, characterized in that: The first edge (31) and the second edge (32) are arranged at an angle of 60 degrees.
3. The reagent centrifugal separation device for experiments according to claim 1, characterized in that: The bottom edge (33) is connected with a long plate (35) on the lower side, and the long plate (35) is connected with a shaft sleeve (34) on the top surface.
4. The reagent centrifugal separation device for experiments according to claim 1, characterized in that: The fixing device (4) includes a first side baffle (41), a second side baffle (42), a bottom plate (43), and a lower baffle (44). The bottom plate (43) is a square plate with a long side extending downward from top to bottom. The first side baffle (41), the second side baffle (42), and the lower baffle (44) are connected to the upper side of the bottom plate (43). The first side baffle (41) and the second side baffle (42) are arranged along the long side of the bottom plate (43). The hole plate (5) is clamped between the first side baffle (41) and the second side baffle (42). The lower baffle (44) is arranged along the short side of the bottom plate (43) on the lower side.
5. The reagent centrifugal separation device for experiments according to claim 4, characterized in that: The first side baffle (41) and the second side baffle (42) are provided with clamping grooves (410) on opposite sides. The hole plate (5) is clamped in the clamping grooves (410) on both sides.
6. The reagent centrifugal separation device for experiments according to claim 5, characterized in that: The first side baffle (41) is provided with a first inclined surface (411) at the end away from the bottom plate (43). The first inclined surface (411) is inclined towards the clamping groove (410) provided on the first side baffle (41). The second side baffle (42) is provided with a second inclined surface (421) at the end away from the bottom plate (43). The second inclined surface (421) is inclined towards the clamping groove (410) provided on the second side baffle (42).
7. The reagent centrifugal separation device for experiments according to claim 1, characterized in that: The bottom surface of the separating cavity (11) is provided with a liquid collecting groove (111). The cross section of the liquid collecting groove (111) is in the shape of an arc. The bottom surface of the separating cavity (11) is inclined towards the liquid collecting groove (111). A plurality of liquid discharge holes (112) are provided at the lowest part of the liquid collecting groove (111).
8. The reagent centrifugal separation device for experiments according to claim 7, characterized in that: The lower side of the separating cavity (11) is provided with a liquid collecting box (13). The liquid collecting box (13) is in communication with the liquid discharge holes (112).
9. The reagent centrifugal separation device for experiments according to claim 1, characterized in that: A liquid blocking part (14) is provided at the center of the separating cavity (11). The liquid blocking part (14) is arranged around the rotating shaft (12).
10. The reagent centrifugal separation device for experiments according to claim 1, characterized in that: The orifice plate (5) is provided with a liquid guide groove (51) on the top surface, and the liquid guide grooves (51) are arranged in a cross shape along the long and short edges of the orifice plate (5).