Incubator for filamentous fungus research
By setting up isolation membranes and meshes in the culture vessel to divide it into multiple regions, and combining it with isotope labeling technology, the problems of difficult cleaning of the culture vessel and difficulty in studying matrix differences are solved, realizing convenient multi-matrix research and cleaning, and ensuring the accuracy of experimental results.
Patent Information
- Application Number
- CN202520043065.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-08
- Publication Date
- 2026-01-02
- Estimated Expiration
- 2035-01-08
AI Technical Summary
Existing culture vessels are difficult to clean thoroughly, resulting in residual hyphae or their metabolites inside, which affects experimental results. Furthermore, there is a lack of equipment for studying the interactions between filamentous fungi, especially in different substrates.
A culture device comprising an isolation membrane and a mesh was designed, divided into multiple zones, allowing the addition of different substrates and hyphae in each zone. By combining isotope labeling technology, the growth and element uptake of hyphae in different substrates were studied.
This method enables the study of filamentous fungal growth and element migration in multiple matrices, overcoming the limitations of single-matrix studies. Furthermore, the removable mesh and isolation membrane facilitate cleaning, ensuring the accuracy of experimental results.
Smart Images
Figure CN223752747U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the technical field of fungus research, and specifically relates to a culture device for filamentous fungus research. BACKGROUND
[0002] Filamentous fungi refer to fungi with filamentous structures, which are called hyphae. Hyphae are tubular, septate cells that can grow and spread in organic matter and the environment. Filamentous fungi include some common types, such as molds, penicillium, and aspergillus, and the cultivation of filamentous fungi usually requires special culture devices (such as culture dishes, shake flasks, and fermentation tanks) to provide suitable growth environments. These culture devices help control temperature, humidity, light, pH, and nutrient supply.
[0003] Existing culture devices are difficult to disassemble and clean separately. The byproducts of filamentous fungus growth and the nutrients that may remain can easily form difficult-to-remove dirt or biofilms on the inner walls and other internal components of the culture device. Incomplete cleaning may result in residual hyphae or their metabolites from previous cultures in the culture device, affecting subsequent experiments. Existing culture devices lack equipment for studying the mutual influence between filamentous fungi, especially equipment for studying the mutual influence between filamentous fungi in different substrates. SUMMARY
[0004] The utility model aims to provide a culture device for filamentous fungus research to solve the problems in the background art.
[0005] To achieve the above-mentioned purpose, the utility model provides the following technical scheme:
[0006] A culture device for filamentous fungus research, comprising:
[0007] A culture box;
[0008] A plurality of isolation membranes, which are arranged inside the culture box;
[0009] Further comprising a plurality of isolation nets, which divide the culture box into a central inoculation area, a first substrate area, a second substrate area, a third substrate area, and a single-sided inoculation area. The central inoculation area is arranged at the center of the culture box, and is isolated by two isolation nets. The first substrate area is arranged on both sides of the central inoculation area. The second substrate area is arranged on one side of the first substrate area away from the central inoculation area. The third substrate area is arranged on one side of the second substrate area. The single-sided inoculation area is arranged on one side of the third substrate area. The central inoculation area, the first substrate area, the second substrate area, the third substrate area, and the single-sided inoculation area are all divided by isolation nets.
[0010] The culture box is provided with a fixing assembly for fixing the isolation nets.
[0011] On the basis of the above technical solutions, the utility model further provides the following optional technical solutions:
[0012] In an optional solution, the fixed component includes a sliding groove and a wedge-shaped groove, the sliding groove is opened on both sides of the top of the screen, the wedge-shaped groove is opened on both sides of the screen, a wedge-shaped block is slidably connected inside the wedge-shaped groove, a fixed column is installed on one side of the wedge-shaped block, a pressing plate is installed at one end of the fixed column, the pressing plate is slidably connected inside the sliding groove, a spring is installed on one side of the pressing plate, one end of the spring is installed on the inner wall of the sliding groove, and the inner walls on both sides of the culture box and both sides of the isolation film are provided with clamping grooves corresponding to the positions of the wedge-shaped blocks.
[0013] In an optional solution, sliding rails are installed on both sides of the isolation film, and the culture box is internally provided with a plurality of sliding grooves corresponding to the positions of the sliding rails.
[0014] In an optional solution, the screen is a double-layer isolation screen with a pore size of 1 mm.
[0015] In an optional solution, the isolation film is a filter film with a pore size of 0.45 um.
[0016] In an optional solution, the top of the culture box is provided with a top cover.
[0017] In an optional solution, the isolation film can divide the culture box into two or three areas.
[0018] In an optional solution, the first substrate area, the second substrate area, the third substrate area, and the single-sided inoculation area are symmetrically distributed around the central inoculation area.
[0019] Compared with the prior art, the utility model has the following beneficial effects:
[0020] 1. The utility model discloses a screen and an isolation film are divided into multiple areas, and target mycelial fungi, companion mycelial fungi and different substrates are respectively added in these areas, so that the growth of target mycelia in each substrate, the absorption of C or N elements in each substrate, the migration of C or N elements in the mycelium during the growth of the mycelium, and the influence of the arrangement order of the substrates can be studied, thereby breaking through the limitation that one isotope labeling can only be applied in one substrate study, realizing the demand that one isotope labeling technology is applied in two or more substrate studies, and combining the technology with a culture box.
[0021] 2. The screen and the isolation film of the utility model can be detached, so that the screen and the isolation film can be taken down after the study is completed, and the screen, the isolation film and the culture box can be cleaned respectively. BRIEF DESCRIPTION OF DRAWINGS
[0022] Figure 1 It is a structure schematic view of the utility model.
[0023] Figure 2 It is a structure schematic view of the utility model.
[0024] Figure 3 It is a top view structure schematic view of the utility model.
[0025] Figure 4 It is a partial cut structure schematic view of the utility model
[0026] Figure 5 It is the utility model Figure 4 Enlarged structure schematic view of A.
[0027] Among them: 100, culture box;200, isolation film;301, isolation net;302, central inoculation area;303, first matrix area;304, second matrix area;305, third matrix area;306, unilateral inoculation area;401, sliding groove;402, wedge-shaped groove;403, wedge-shaped block;404, fixed column;405, pressing plate;406, spring;407, clamping groove;501, sliding rail;502, sliding groove;601, top cover. DETAILED DESCRIPTION
[0028] In order to make the purpose, technical scheme and advantage of the utility model more clearly, the following will be further detailed in combination with the drawings and examples.
[0029] In one embodiment, as Figures 1-5 shown, a culture device for filamentous fungi research includes: a culture box 100, a plurality of isolation films 200 and a plurality of isolation nets 301, the isolation film 200 is arranged in the inside of the culture box 100, a plurality of the isolation net 301 divides the culture box 100 into central inoculation area 302, first matrix area 303, second matrix area 304, third matrix area 305 and unilateral inoculation area 306, the central inoculation area 302 is arranged at the central position in the inside of the culture box 100, the central inoculation area 302 is formed by two isolation nets 301, the first matrix area 303 is arranged at the two sides of the central inoculation area 302, the second matrix area 304 is arranged at the side of the first matrix area 303 away from the central inoculation area 302, the third matrix area 305 is arranged at the side of the second matrix area 304, the unilateral inoculation area 306 is arranged at the side of the third matrix area 305, the central inoculation area 302, first matrix area 303, second matrix area 304, third matrix area 305 and unilateral inoculation area 306 are all divided into areas by the isolation net 301;By adding the same kind of matrix in the inside of the first matrix area 303 and the third matrix area 305, adding another kind of matrix in the inside of the second matrix area 304, it is convenient for the experimental personnel to carry out experiments;
[0030] The culture box 100 is provided with a fixing assembly for fixing the partition net 301.
[0031] In one embodiment, as shown in Figure 4 and Figure 5 , the fixing assembly comprises a sliding groove 401 and a wedge-shaped groove 402, the sliding groove 401 is opened at both sides of the top of the partition net 301, the wedge-shaped groove 402 is opened at both sides of the partition net 301, the wedge-shaped block 403 is slidably connected inside the wedge-shaped groove 402, the fixing column 404 is installed on one side of the wedge-shaped block 403, the pressing plate 405 is installed at one end of the fixing column 404, the pressing plate 405 is slidably connected inside the sliding groove 401, the spring 406 is installed on one side of the pressing plate 405, one end of the spring 406 is installed on the inner wall of the sliding groove 401, the inner walls of both sides of the culture box 100 and both sides of the isolation film 200 are provided with clamping grooves 407 corresponding to the position of the wedge-shaped block 403; by simultaneously pressing the pressing plate 405, the pressing plate 405 extrudes the spring 406, and the fixing column 404 and the wedge-shaped block 403 are slid in the wedge-shaped groove 402, the wedge-shaped block 403 is withdrawn into the wedge-shaped groove 402, and the partition net 301 is installed between the culture box 100 and the isolation film 200, the wedge-shaped block 403 is aligned with the position of the clamping groove 407, at this time, under the action of the spring 406, the wedge-shaped block 403 is reset and clamped into the clamping groove 407, so that the partition net 301 is fixed.
[0032] In one embodiment, as shown in Figure 4 , the isolation film 200 is provided with sliding rails 501 on both sides, and the culture box 100 is provided with sliding grooves 502 corresponding to the position of the sliding rails 501; by aligning the sliding rails 501 on the isolation film 200 with the sliding grooves 502, the isolation film 200 is installed inside the culture box 100.
[0033] In one embodiment, as shown in Figure 2 , Figure 3 and Figure 4 , the aperture of the partition net 301 is a double-layer isolation net with an aperture of 1mm; to ensure ventilation and material diffusion.
[0034] In one embodiment, as shown in Figure 2 , Figure 3 and Figure 4 , the isolation film 200 is a filter film with an aperture of 0.45um; to effectively block the diffusion of associated bacteria or other microorganisms.
[0035] In one embodiment, as shown in Figure 1 , the top of the culture box 100 is provided with a top cover 601.
[0036] In one embodiment, as shown in Figure 2 and Figure 3 The isolation film 200 can divide the culture box 100 into two or three areas; the target mycelium can be inoculated in the middle of the area isolated by the isolation film 200, and a single accompanying mycelium or two different accompanying mycelia can be inoculated on both sides of the area isolated by the isolation film 200, and the accompanying filamentous fungi can live in the immediate substrate through the 0.45 μm isolation film.
[0037] In one embodiment, as shown in Figure 1 The first substrate area 303, the second substrate area 304, the third substrate area 305, and the single-side inoculation area 306 are symmetrically distributed around the central inoculation area 302; this facilitates observation by the experimenter.
[0038] The above embodiment discloses a culture device for filamentous fungi research. In use, first, the isolation film 200 and the isolation net 301 are installed by aligning the slide rail 501 on the isolation film 200 with the slide groove 502, then the isolation film 200 is installed inside the culture box 100, then the press plate 405 is simultaneously actuated to cause the press plate 405 to compress the spring 406, and the fixed column 404 and the wedge block 403 are caused to slide in the wedge-shaped groove 402 by the press plate 405, so that the wedge block 403 is withdrawn into the wedge-shaped groove 402, and the isolation net 301 is installed between the culture box 100 and the isolation film 200, so that the wedge block 403 is aligned with the position of the clamping groove 407, at this time the wedge block 403 is reset under the action of the spring 406 and clamped into the clamping groove 407, so that the isolation net 301 is fixed, and the culture box 100 can be divided into multiple areas for research. At this time, the research can be carried out by two methods. The first research method is the central inoculation method, that is, the target mycelium is inoculated in the center of the three areas isolated by the isolation film 200, the accompanying mycelium is inoculated on both sides of the isolated area of the isolation film 200, the same substrate is added to the inside of the first substrate area 303 and the third substrate area 305, and another substrate is added to the inside of the second substrate area 304, so as to study the mutual influence of the mycelium growth in the same direction. The second method is the central-bilateral inoculation method, that is, the target mycelium is inoculated in the center of the three areas isolated by the isolation film 200, and the accompanying mycelium is inoculated in the inside of the single-side inoculation area 306 on both sides of the culture box 100, so as to study the mutual influence when the mycelium grows in the opposite direction, and each mycelium only experiences one substrate with an isotopic label. Then the properties of the substrate in each substrate area are sampled, and the isotopic labeling technique and the subtraction method are used in combination to clearly study the growth of the target mycelium in each substrate, the absorption of C or N elements in each substrate, the migration of C or N elements in the mycelium during the growth of the mycelium, and the influence of the substrate arrangement order.
[0039] The above merely provides the specific implementation of the present application, but the protection scope of the present application is not limited to this. Any person skilled in the art can easily think of the changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.
Claims
1. A culture device for filamentous fungi research, comprising: a culture box (100); a plurality of isolation membranes (200) arranged inside the culture box (100); characterized in that it further comprises a plurality of isolation nets (301), which divide the culture box (100) into a central inoculation area (302), a first substrate area (303), a second substrate area (304), a third substrate area (305), and a single-sided inoculation area (306); the central inoculation area (302) is arranged at the center of the culture box (100) and is formed by two isolation nets (301); the first substrate area (303) is arranged on both sides of the central inoculation area (302); the second substrate area (304) is arranged on the side of the first substrate area (303) away from the central inoculation area (302); the third substrate area (305) is arranged on the side of the second substrate area (304); the single-sided inoculation area (306) is arranged on the side of the third substrate area (305); and the central inoculation area (302), the first substrate area (303), the second substrate area (304), the third substrate area (305), and the single-sided inoculation area (306) are all divided by isolation nets (301). The culture box (100) is provided with a fixing assembly for fixing the isolation nets (301).
2. The filamentous fungus research culture device according to claim 1, characterized by The fixing assembly comprises sliding grooves (401) and wedge-shaped grooves (402); the sliding grooves (401) are arranged on both sides of the top of the isolation net (301); the wedge-shaped grooves (402) are arranged on both sides of the isolation net (301); the wedge-shaped grooves (402) are slidably connected with wedge-shaped blocks (403); one side of the wedge-shaped block (403) is provided with a fixing column (404); one end of the fixing column (404) is provided with a pressing plate (405); the pressing plate (405) is slidably connected inside the sliding groove (401); one side of the pressing plate (405) is provided with a spring (406); one end of the spring (406) is fixed to the inner wall of the sliding groove (401); and the inner walls of both sides of the culture box (100) and both sides of the isolation membrane (200) are provided with clamping grooves (407) corresponding to the positions of the wedge-shaped blocks (403).
3. The filamentous fungus research culture device according to claim 1, characterized by Both sides of the isolation membrane (200) are provided with sliding rails (501), and the inside of the culture box (100) is provided with a plurality of sliding grooves (502) corresponding to the positions of the sliding rails (501).
4. The filamentous fungus research culture device according to claim 2, characterized by The isolation net (301) is a double-layer isolation net with a pore size of 1 mm.
5. The filamentous fungus research culture device according to claim 1, characterized by The isolation membrane (200) is a filter membrane with a pore size of 0.45 um.
6. The filamentous fungus research culture device according to claim 1, wherein The top of the culture box (100) is provided with a top cover (601).
7. The filamentous fungus research culture device according to claim 1, wherein The isolation membrane (200) divides the culture box (100) into two or three areas.
8. The filamentous fungus research culture device according to claim 2, characterized by The first substrate area (303), the second substrate area (304), the third substrate area (305), and the single-sided inoculation area (306) are symmetrically distributed around the central inoculation area (302).