Intermittent perfusion fed-batch cell culture device

Abstract

The utility model discloses an intermittent perfusion fed-batch cell culture device which comprises a protection box, and a fixed plate is fixedly mounted in the protection box; the telescopic hydraulic cylinder is installed on the side face of the left side fixing plate through screws. The box cover is mounted at the upper end of the telescopic hydraulic cylinder through a connecting sleeve and a first screw; the sliding rod is mounted below the box cover through a second screw, and the sliding rod is in sliding contact with the right side fixing plate; the connecting seat is fixedly connected to the lower part of the box cover; the culture box is mounted on the side surface of the connecting seat through a mounting part, and the mounting part comprises a fixed cylinder, a threaded rod, a rotating rod, an abutting block, a sliding block and a limiting bolt; the internal thread pipe is fixedly mounted above the box cover; the material supplementing assembly is in threaded connection with the inner threaded pipe, and the material supplementing assembly comprises a material supplementing barrel, a push-out rod, an outer threaded pipe, a discharging pipe and a perfusion pipe. The cell culture is relatively closed, and bacteria can be prevented from invading and polluting an internal culture solution.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture device field, concretely relates to intermittent perfusion batch feeding cell culture device. BACKGROUND

[0002] Cells need to use cell culture devices for culture in the process of culture, such as patent application number: 201821554831.3, cell culture device is disclosed, including slide and separating device, the upper surface of the slide is formed with multiple cell culture surfaces, the edge of each cell culture surface is formed respectively, the edge recess of the edge recess formed by the upper surface of the slide to the lower surface direction of the slide;The separating device has a plurality of surrounding plates connected with each other, a plurality of surrounding plates and a plurality of edge recesses one by one, in a group of corresponding surrounding plates and the edge recess: the lower end of the surrounding plate is inserted into the interior of the edge recess, so that the cell culture surface is surrounded, and sealing components are arranged between the lower end of the surrounding plate and the inner wall of the edge recess. It has the advantages of convenient disassembly, durable, easy to clean and disinfect.

[0003] But the patent still has the following defects: in order to optimize the culture environment and improve the culture effect, usually batch feeding is intermittently added to the separating device, to adjust the PH value of the culture solution, so the cover plate needs to be opened frequently, in the process of opening, bacteria are easy to invade the separating device, and the internal culture solution is contaminated. UTILITY MODEL CONTENT

[0004] In view of the above defects or deficiencies in the prior art, it is desirable to provide intermittent perfusion batch feeding cell culture device, by the setting of PH value detection tube and second sealing cover, internal thread pipe and feeding assembly, carbon dioxide detection tube and first sealing cover, the cell culture is relatively closed, and bacteria can be avoided to invade and contaminate the internal culture solution.

[0005] According to the technical scheme provided by the embodiment of the application, the intermittent perfusion fed-batch cell culture device comprises a protection box, a fixed plate is fixedly installed in the inside of the protection box, a telescopic hydraulic cylinder is installed on the side of the fixed plate on the left through a screw, a box cover is installed on the upper end of the telescopic hydraulic cylinder through a connecting sleeve and a first screw, a sealing gasket is bonded to the bottom of the box cover, the sealing gasket is located between the box cover and the protection box, a sliding rod is installed below the box cover through a second screw, the sliding rod is in sliding contact with the fixed plate on the right, the side of the fixed plate on the right is provided with a sliding hole corresponding to the sliding rod, the sliding rod passes through the sliding hole, a fixed disc is fixedly installed at the top end of the sliding rod, the surface of the fixed disc is provided with a first perforation corresponding to the second screw, a connecting seat is fixedly connected below the box cover, the connecting seat is located between the fixed plates on the left and the right, a culture box is installed on the side of the connecting seat through a mounting piece, the mounting piece comprises a fixed cylinder, a threaded rod, a rotating rod, an abutment block, a sliding block and a limiting bolt, the protection box and the culture box are both of an open-top structure, a first transparent observation window is embedded in the front side of the protection box, a second transparent observation window is embedded in the front side of the culture box, the fixed cylinder is fixedly installed on the side of the connecting seat, the threaded rod is screwed in the inside of the fixed cylinder, the inside of the fixed cylinder is provided with an internal thread matching the threaded rod, the rotating rod is fixedly connected to the side of the threaded rod, the abutment block is installed at the end of the threaded rod through a bearing, the sliding block is fixedly installed below the abutment block, the surface of the connecting seat is provided with a sliding groove matching the sliding block, the bottom end of the sliding block extends into the sliding groove, the end of the limiting bolt penetrates into one side of the abutment block, one side of the abutment block is provided with a first screw groove matching the limiting bolt, the side of the rotating rod is provided with a second perforation corresponding to the limiting bolt, the other side of the abutment block is bonded with a first anti-skid pad, the first anti-skid pad is abutted to the left and right sides of the culture box respectively, an internal thread pipe is fixedly installed above the box cover, a feeding assembly is screwed with the internal thread pipe, the feeding assembly comprises a feeding cylinder, a pushing rod, an external thread pipe, a discharge pipe and a perfusion pipe.The surface of the box cover is provided with a first through hole corresponding to the perfusion pipe, the perfusion pipe passes through the first through hole, the outer threaded pipe is screwed with the inner threaded pipe, the feeding cylinder is fixedly installed above the outer threaded pipe, the push-out rod comprises a push rod and a piston head, the piston head is fixedly installed at the bottom end of the push rod, the piston head is in sliding contact with the inner wall of the feeding cylinder, the discharge pipe is fixedly connected below the feeding cylinder, the perfusion pipe is sleeved outside the discharge pipe, a detection device fixing member is installed above the box cover, the detection device fixing member comprises a supporting plate, a guide block, a positioning rod, a limiting screw and a moving plate; the supporting plate is fixedly installed above the box cover, the guide block is fixedly installed above the supporting plate, the positioning rod is in sliding contact with the guide block and the supporting plate, the side surface of the guide block is provided with a sliding opening matched with the positioning rod, the positioning rod passes through the sliding opening, the top of the positioning rod is provided with a second screw groove matched with the limiting screw in intervals, the surface of the guide block is provided with a third through hole corresponding to the limiting screw, the end of the limiting screw is screwed into the second screw groove through the third through hole, the moving plate is fixedly installed below the positioning rod, the side surface of the moving plate is bonded with a second anti-skid pad, the bottom of the moving plate is in sliding contact with the box cover, a support is fixedly connected above the culture box, and the surface of the support is penetrated and installed with a first connecting pipe and a second connecting pipe; the second connecting pipe and the first connecting pipe are both hollow structures, a carbon dioxide detection pipe is fixedly connected to the side surface of the box cover, and a first cover is screwed to the outside of the carbon dioxide detection pipe; a carbon dioxide supplement pipe is fixedly connected to the side surface of the box cover; the surface of the box cover is provided with a third through hole corresponding to the carbon dioxide detection pipe, and the surface of the box cover is provided with a fourth through hole corresponding to the carbon dioxide supplement pipe, the carbon dioxide supplement pipe passes through the fourth through hole, and the bottom ends of the carbon dioxide supplement pipe and the perfusion pipe both pass through the first connecting pipe, a PH value detection pipe is fixedly connected to the side surface of the box cover, a second cover is screwed to the outside of the PH value detection pipe, the surface of the box cover is provided with a second through hole corresponding to the PH value detection pipe, and the second through hole is located above the second connecting pipe.

[0006] In summary, the beneficial effects of the present application are:

[0007] 1. By arranging the PH value detection pipe and the second cover, the inner threaded pipe and the feeding assembly, and the carbon dioxide detection pipe and the first cover, the cell culture is relatively closed, and bacterial invasion and contamination of the internal culture solution can be avoided.

[0008] 2、Through the setting of the telescopic hydraulic cylinder, the box cover and the mounting piece, the culture box can be conveniently disassembled, so that the culture box is convenient to clean;

[0009] 3、Through the setting of the detection device fixing piece, the product also has the function of fixing the detection device, which is convenient for subsequent intermittent detection of the culture solution PH value;

[0010] 4、Through the setting of the carbon dioxide detection pipe and the carbon dioxide supplement pipe, the detection and supplement of carbon dioxide are facilitated. BRIEF DESCRIPTION OF DRAWINGS

[0011] Other characteristics, objects and advantages of the present application will become more apparent from the following detailed description of non-restrictive embodiments made with reference to the attached drawings:

[0012] Figure 1 It is a structural schematic view of the utility model;

[0013] Figure 2 It is a structural schematic view of the connecting seat, the culture box and the mounting piece connecting part of the utility model;

[0014] Figure 3 It is a structural schematic view of the fixing plate, the telescopic hydraulic cylinder, the box cover and the sliding rod connecting part of the utility model;

[0015] Figure 4 It is a top view structural schematic view of the detection device fixing piece of the utility model;

[0016] Figure 5 It is a structural schematic view of the material supplement assembly of the utility model.

[0017] Reference signs in the drawings: 1, protective box; 2, fixing plate; 3, telescopic hydraulic cylinder; 4, box cover; 5, sliding rod; 6, connecting seat; 7, culture box; 8, mounting piece; 9, fixed cylinder; 10, threaded rod; 11, rotating rod; 12, abutting block; 13, sliding block; 14, limiting bolt; 15, inner threaded pipe; 16, material supplement assembly; 17, material supplement cylinder; 18, push-out rod; 19, outer threaded pipe; 20, discharge pipe; 21, perfusion pipe; 22, detection device fixing piece; 23, supporting plate; 24, guide block; 25, positioning rod; 26, limiting screw; 27, moving plate; 28, support; 29, first butt joint pipe; 30, second butt joint pipe; 31, carbon dioxide detection pipe; 32, carbon dioxide supplement pipe; 33, first sealing cover; 34, PH value detection pipe; 35, second sealing cover. DETAILED DESCRIPTION

[0018] The application will be described in further detail below with reference to the drawings and embodiments. It can be understood that the specific embodiments described herein are only used to explain the related utility model, and not to limit the utility model. In addition, it should be noted that only parts related to the utility model are shown in the drawings for ease of description.

[0019] It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict. The present application will be described in detail below with reference to the drawings and embodiments.

[0020] Please refer to Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 , intermittent perfusion fed-batch cell culture device, including protective box 1, the inside of the protective box 1 is fixedly installed with fixed plate 2;Telescopic hydraulic cylinder 3, the telescopic hydraulic cylinder 3 is installed on the side of the left fixed plate 2 by screw;Box cover 4, the box cover 4 is installed on the upper end of the telescopic hydraulic cylinder 3 by connecting sleeve and first screw;Slide rod 5, the slide rod 5 is installed below the box cover 4 by second screw, the slide rod 5 is in sliding contact with the right fixed plate 2;Connecting seat 6, the connecting seat 6 is fixedly connected below the box cover 4;Culture box 7, the culture box 7 is installed on the side of the connecting seat 6 through mounting piece 8, the mounting piece 8 includes fixed cylinder 9, threaded rod 10, rotating rod 11, abutment block 12, sliding block 13 and limiting bolt 14;Internal thread pipe 15, the internal thread pipe 15 is fixedly installed above the box cover 4;Feed assembly 16, the feed assembly 16 is screwed with the internal thread pipe 15, the feed assembly 16 includes feed cylinder 17, push-out rod 18, external thread pipe 19, discharge pipe 20 and perfusion pipe 21;Detection device fixing piece 22, the detection device fixing piece 22 is installed above the box cover 4, the detection device fixing piece 22 includes bracing plate 23, guide block 24, positioning rod 25, limiting screw 26 and moving plate 27;Support 28, the support 28 is fixedly connected above the culture box 7, the surface of the support 22 is installed through first docking pipe 29 and second docking pipe 30;Carbon dioxide detection pipe 31, the carbon dioxide detection pipe 31 is fixedly connected on the side of the box cover 4, the outside of the carbon dioxide detection pipe 31 is screwed with first cover 33;Carbon dioxide supplement pipe 32, the carbon dioxide supplement pipe 32 is fixedly connected on the side of the box cover 4;PH value detection pipe 34, the PH value detection pipe 34 is fixedly connected on the side of the box cover 4, the outside of the PH value detection pipe 34 is screwed with second cover 35.

[0021] As Figure 1 , Figure 2 and Figure 3As shown, the front side of the protection box 1 is provided with a control switch, the telescopic hydraulic cylinder 3 is electrically connected with the control switch, which is convenient for control, the protection box 1 and the culture box 7 are both top open structure, the front side of the protection box 1 is embedded with a first transparent observation window, the front side of the culture box 7 is embedded with a second transparent observation window, which is convenient for observing the cell culture condition, the bottom of the box cover 4 is bonded with a sealing washer, and the sealing washer is located between the box cover 4 and the protection box 1. The side surface of the right fixing plate 2 is provided with a sliding through hole corresponding to the sliding rod 5, which is convenient for sliding of the sliding rod 5, the sliding rod 5 penetrates through the sliding through hole, and the top end of the sliding rod 5 is fixedly installed with a fixing disc, which is convenient for installation of the sliding rod 5, and the surface of the fixing disc is provided with a first through hole corresponding to the second screw.

[0022] As shown in Figure 1 , Figure 2 , Figure 3 and Figure 5 , the connecting seat 6 is located between the left and right fixing plates 2, the surface of the box cover 4 is provided with a first through hole corresponding to the perfusion pipe 21, which is convenient for penetration of the perfusion pipe 21, and the perfusion pipe 21 penetrates through the first through hole. The fixing cylinder 9 is fixedly installed on the side surface of the connecting seat 6, the threaded rod 10 is screwed in the inner portion of the fixing cylinder 9, the inner portion of the fixing cylinder 9 is provided with an internal thread matched with the threaded rod 10, the rotating rod 11 is fixedly connected on the side surface of the threaded rod 10, the abutting block 12 is installed on the end portion of the threaded rod 10 through a bearing, the sliding block 13 is fixedly installed below the abutting block 12, the surface of the connecting seat 6 is provided with a sliding groove matched with the sliding block 13, which is convenient for sliding of the sliding block 13, the bottom end of the sliding block 13 extends into the sliding groove, the end portion of the limiting bolt 14 penetrates through the rotating rod 11 and is screwed into one side of the abutting block 12, one side of the abutting block 12 is provided with a first screw groove matched with the limiting bolt 14, the first screw groove is arranged in an annular array, and a spacing is arranged between adjacent first screw grooves, the side surface of the rotating rod 11 is provided with a second through hole corresponding to the limiting bolt 14, and the other side of the abutting block 12 is bonded with a first anti-skid pad, and the first anti-skid pad is respectively abutted on the left and right sides of the culture box 7.

[0023] As shown in Figure 1 , Figure 4 and Figure 5As shown, the externally threaded tube 19 is screwed to the internally threaded tube 15. The feeding cylinder 17 is fixedly installed above the externally threaded tube 19. The push rod 18 includes a push rod and a piston head. The piston head is fixedly installed at the bottom end of the push rod. The piston head slides in contact with the inner wall of the feeding cylinder 17. The feeding cylinder 17 is made of transparent material. The side of the feeding cylinder 17 is engraved with scale lines. The discharge pipe 20 is fixedly connected below the feeding cylinder 17. The diameter of the discharge pipe 20 is smaller than the diameter of the externally threaded tube 19. The irrigation tube 21 is sleeved on the outside of the discharge pipe 20. The support plate 23 is fixedly installed above the box cover 4, the guide block 24 is fixedly installed above the support plate 23, the positioning rod 25 slides in contact with the guide block 24 and the support plate 23, the side of the guide block 24 is provided with a sliding opening that matches the positioning rod 25, which facilitates the passage of the positioning rod 25, the positioning rod 25 passes through the sliding opening, the top of the positioning rod 25 is provided with a second threaded groove that matches the limiting screw 26, the surface of the guide block 24 is provided with a third through hole that corresponds to the limiting screw 26, which facilitates the installation of the limiting screw 26, the end of the limiting screw 26 passes through the third through hole and is screwed into the second threaded groove, the moving plate 27 is fixedly installed below the positioning rod 25, the side of the moving plate 27 is adhered with a second anti-slip pad, and the bottom of the moving plate 27 slides in contact with the box cover 4.

[0024] like Figure 1 As shown, the surface of the box cover 4 is provided with a second through hole corresponding to the pH value detection tube 34. The second through hole is located above the second pair of connecting tubes 30. Both the second pair of connecting tubes 30 and the first pair of connecting tubes 29 are hollow structures. The surface of the box cover 4 is provided with a third through hole corresponding to the carbon dioxide detection tube 31. The surface of the box cover 4 is provided with a fourth through hole corresponding to the carbon dioxide replenishment tube 32. The carbon dioxide replenishment tube 32 passes through the fourth through hole. The bottom ends of both the carbon dioxide replenishment tube 32 and the irrigation tube 21 pass through the first pair of connecting tubes 29.

[0025] In use: first add glucose into the feeding cylinder 17 of the feeding assembly 16 on one side, add urea dissolved in water into the feeding cylinder 17 of the feeding assembly 16 on the other side, connect the carbon dioxide supplement pipe 32 with the carbon dioxide compressor using the pipeline, and then place the detection device between the left and right detection device fixing members 22, the detection device can refer to the acidity meter disclosed in patent application No. 202320799542.4, then unscrew the limiting screw 26, move the positioning rod 25, so that the second anti-skid pads on the moving plate 27 abut against the left and right sides of the detection device respectively, and then screw the end of the limiting screw 26 into the second screw groove in the corresponding position of the positioning rod 25, so that the product also has the function of fixing the detection device, which facilitates the subsequent intermittent detection of the PH value of the culture solution. During the cultivation process of the culture box 7, the second cover 35 of the PH value detection pipe 34 can be unscrewed, the electrode of the detection device can pass through the PH value detection pipe 34 and the second connecting pipe 30 and extend into the culture box 7, so that the PH value of the culture solution can be detected. When the PH value is too high or too low, the push-out rod 18 can be pushed out, which can push the feed in the feeding cylinder 17 out, and the feed can be fed into the culture box 7 in batches through the discharge pipe 20 and the perfusion pipe 21, so that the PH value can be adjusted. At the same time, the first cover 33 of the carbon dioxide detection pipe 31 is unscrewed, the gas inlet pipe of the carbon dioxide concentration detection device is inserted into the carbon dioxide detection pipe 31, and the concentration of carbon dioxide can be detected intermittently. The carbon dioxide concentration detection device can refer to the carbon dioxide concentration detection device disclosed in patent application No. 202420305043.X. When the concentration of carbon dioxide is low, carbon dioxide can be supplemented into the protective box 1 through the carbon dioxide compressor, so that the cultivation environment can be optimized and the cultivation effect can be improved. During the process of supplementing carbon dioxide, the gas pressure balance in the protective box 1 can be maintained through the open carbon dioxide detection pipe 31. Through the settings of the PH value detection pipe 34 and the second cover 35, the inner threaded pipe 15 and the feeding assembly 16, the carbon dioxide detection pipe 31 and the first cover 33, the cell culture is relatively closed, which can avoid bacterial invasion and contamination of the internal culture solution. After the cell culture is completed, the electrode is first extracted, the telescopic hydraulic cylinder 3 is started, the box cover 4 is opened, the limiting bolt 14 of the mounting member 8 is unscrewed, and the rotating rod 11 is rotated, which can drive the threaded rod 10 to rotate, so that the abutting block 12 and the sliding block 13 can move away from the culture box 7, so that the culture box 7 can be easily removed, thereby facilitating the cleaning of the culture box 7.

[0026] The above description is only the preferred embodiment of the application and the scheme of the applied technical principles. Meanwhile, the utility model range involved in the application is not limited to the technical scheme formed by the specific combination of the above technical features, and should also cover other technical schemes formed by the combination of the above technical features or equivalent features without departing from the utility model concept. For example, the technical scheme formed by replacing the above features with the technical features disclosed in the application (but not limited to) having similar functions.

Claims

1. A device for intermittent perfusion fed-batch cell culture, characterized in that: Including the protection box (1), the inside fixed mounting of protection box (1) fixed plate (2); Telescopic hydraulic cylinder (3), the telescopic hydraulic cylinder (3) is installed in the left side of the fixed plate (2) by screwing; Box cover (4), the box cover (4) is installed in the upper end of the telescopic hydraulic cylinder (3) through the connecting sleeve and the first screw; Sliding rod (5), the sliding rod (5) is installed below the box cover (4) through the second screw, and the sliding rod (5) is in sliding contact with the right side of the fixed plate (2); Connecting seat (6), the connecting seat (6) is fixedly connected below the box cover (4); Culture box (7), the culture box (7) is installed in the side of the connecting seat (6) through the mounting piece (8), and the mounting piece (8) comprises a fixed cylinder (9), a threaded rod (10), a rotating rod (11), an abutting block (12), a sliding block (13) and a limiting bolt (14); Internal thread pipe (15), the internal thread pipe (15) is fixedly installed above the box cover (4); Feed assembly (16), the feed assembly (16) is screwed with the internal thread pipe (15), and the feed assembly (16) comprises a feed cylinder (17), a push-out rod (18), an external thread pipe (19), a discharge pipe (20) and a perfusion pipe (21); Detection device fixing piece (22), the detection device fixing piece (22) is installed above the box cover (4), and the detection device fixing piece (22) comprises a supporting plate (23), a guide block (24), a positioning rod (25), a limiting screw (26) and a moving plate (27); Support (28), the support (28) is fixedly connected above the culture box (7), and the surface of the support (28) penetrates through the installation of the first butt joint pipe (29) and the second butt joint pipe (30); Carbon dioxide detection pipe (31), the carbon dioxide detection pipe (31) is fixedly connected to the side of the box cover (4), and the outside of the carbon dioxide detection pipe (31) is screwed with the first cover (33); Carbon dioxide supplement pipe (32), the carbon dioxide supplement pipe (32) is fixedly connected to the side of the box cover (4); PH value detection pipe (34), the PH value detection pipe (34) is fixedly connected to the side of the box cover (4), and the outside of the PH value detection pipe (34) is screwed with the second cover (35).

2. The device for intermittent perfusion fed-batch cell culture according to claim 1, characterized in that: The protection box (1) and the culture box (7) are both open at the top, the front side of the protection box (1) is embedded with a first transparent observation window, the front side of the culture box (7) is embedded with a second transparent observation window, the bottom of the box cover (4) is bonded with a sealing washer, and the sealing washer is located between the box cover (4) and the protection box (1).

3. The device for intermittent perfusion fed-batch cell culture according to claim 1, characterized in that: The side of the right side of the fixed plate (2) is provided with a sliding hole corresponding to the sliding rod (5), the sliding rod (5) penetrates through the sliding hole, and the top end of the sliding rod (5) is fixedly installed with a fixed disc, and the surface of the fixed disc is provided with a first perforation corresponding to the second screw.

4. The device for intermittent perfusion fed-batch cell culture according to claim 1, characterized in that: The connecting seat (6) is located between the left and right fixing plates (2), the surface of the box cover (4) is provided with a first through hole corresponding to the perfusion pipe (21), and the perfusion pipe (21) penetrates through the first through hole.

5. The device of claim 1, wherein: The fixed cylinder (9) is fixedly installed on the side of the connecting seat (6), the threaded rod (10) is screwed in the inside of the fixed cylinder (9), the inside of the fixed cylinder (9) is provided with an internal thread matched with the threaded rod (10), the rotating rod (11) is fixedly connected to the side of the threaded rod (10), the abutting block (12) is installed on the end of the threaded rod (10) through a bearing, the sliding block (13) is fixedly installed below the abutting block (12), the surface of the connecting seat (6) is provided with a sliding groove matched with the sliding block (13), the bottom end of the sliding block (13) extends into the sliding groove, the end of the limiting bolt (14) penetrates through the rotating rod (11) and is screwed into one side of the abutting block (12), one side of the abutting block (12) is provided with a first screw groove matched with the limiting bolt (14), the side of the rotating rod (11) is provided with a second through hole corresponding to the limiting bolt (14), the other side of the abutting block (12) is bonded with a first anti-skid pad, and the first anti-skid pad is abutted on the left and right sides of the culture box (7) respectively.

6. The device of claim 1, wherein: The outer threaded pipe (19) is screwed with the inner threaded pipe (15), the feeding cylinder (17) is fixedly installed above the outer threaded pipe (19), the push-out rod (18) comprises a push rod and a piston head, the piston head is fixedly installed at the bottom end of the push rod, the piston head is in sliding contact with the inner wall of the feeding cylinder (17), the discharge pipe (20) is fixedly connected below the feeding cylinder (17), and the perfusion pipe (21) is sleeved on the outer side of the discharge pipe (20).

7. The device of claim 1, wherein the device is configured to perform intermittent perfusion fed-batch cell culture. The supporting plate (23) is fixedly installed above the box cover (4), the guide block (24) is fixedly installed above the supporting plate (23), the positioning rod (25) is in sliding contact with the guide block (24) and the supporting plate (23), the side of the guide block (24) is provided with a sliding opening matched with the positioning rod (25), the positioning rod (25) penetrates through the sliding opening, the top of the positioning rod (25) is provided with a second screw groove matched with the limiting screw (26) at intervals, the surface of the guide block (24) is provided with a third through hole corresponding to the limiting screw (26), the end of the limiting screw (26) penetrates through the third through hole and is screwed into the second screw groove, the moving plate (27) is fixedly installed below the positioning rod (25), the side of the moving plate (27) is bonded with a second anti-skid pad, and the bottom of the moving plate (27) is in sliding contact with the box cover (4).

8. The device of claim 1, wherein: The surface of the box cover (4) is provided with a second through hole corresponding to the PH value detection pipe (34), the second through hole is located above the second butt joint pipe (30), and the second butt joint pipe (30) and the first butt joint pipe (29) are both hollow structures.

9. The device of claim 1, wherein: The surface of the box cover (4) is provided with a third through hole corresponding to the carbon dioxide detection tube (31), and the surface of the box cover (4) is provided with a fourth through hole corresponding to the carbon dioxide supplement tube (32), the carbon dioxide supplement tube (32) passes through the fourth through hole, and the bottom end of the carbon dioxide supplement tube (32) and the perfusion tube (21) passes through the first docking tube (29).

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