Adherent cell culture bottle

Abstract

The utility model relates to the technical field of cell culture bottles, in particular to an adherent cell culture bottle which comprises a bottle body for containing culture solution, a dynamic supporting piece and a stabilizing piece, the dynamic supporting piece is arranged at the bottom of the bottle body and used for being in contact with a table top, and when the bottle body is subjected to external driving force, the dynamic supporting piece is fixed on the bottle body. The dynamic supporting piece is arranged on the bottle body, the dynamic supporting piece drives the bottle body to shake so that a culture solution in the bottle body can be in a uniform and efficient mixed state, and the stabilizing piece is arranged on the bottle body and used for erecting the dynamic supporting piece in a non-operation state so that the bottle body can be stably placed. The bottle body can more smoothly roll on the table top, an operator only needs to slightly apply force to push the bottle body to shake with the dynamic supporting piece as a fulcrum, and compared with a traditional plane bottom culture bottle which needs to be suspended and shaken vigorously in the whole process, the operation difficulty is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture bottle technical field especially is related to a kind of adherent cell culture bottle. BACKGROUND

[0002] In the field of cell biology research and biopharmaceuticals, cell adherent culture is a widely used technical means, and the rationality of the design of cell adherent culture bottle as an important carrier for cell growth plays a key role in cell culture effect.

[0003] In the process of cell culture, in order to enable cells to fully contact the nutrients in the culture medium and promote gas exchange, the culture bottle often needs to be shaken. The traditional cell adherent culture bottle is mostly square and has a flat bottom structure. When shaking, the experimenter needs to manually hold and control the force, frequency and direction throughout the process, which not only requires high hand strength and operation skills of the experimenter, but also easily causes hand fatigue after a long time of operation. The stability of the hand and the control ability of the shaking action will be greatly reduced, which further aggravates the unevenness of shaking and may cause the culture bottle to accidentally slip due to hand fatigue, resulting in spilling of the cell culture medium.

[0004] Based on the above situation, we propose an adherent cell culture bottle to solve the above problems. CONTENT OF THE UTILITY MODEL

[0005] The utility model provides an adherent cell culture bottle to solve the problem of inconvenient hand shaking use of the cell culture bottle in the prior art.

[0006] The technical problem solved by the utility model is realized by the following technical scheme:

[0007] An adherent cell culture bottle includes a bottle body for containing culture medium, a dynamic support and a stabilizing member. The dynamic support is arranged at the bottom of the bottle body and is used to contact the desktop. When the bottle body is driven by an external force, the dynamic support drives the bottle body to produce a shaking motion, realizing uniform and efficient mixing of the culture medium in the bottle body. The stabilizing member is arranged on the bottle body and is used to support the dynamic support in a non-operation state, realizing stable placement of the bottle body.

[0008] Preferably, the dynamic support is a rotating member integrally connected to the bottle body, and the radial cross section of the rotating member is a sector.

[0009] Preferably, the dynamic support is a rotating member integrally connected to the bottle body, and the radial cross section of the rotating member is a sector.

[0010] Preferably, the stabilizing piece comprises a supporting piece connected to the two side flanges of the bottle body by a damping sliding connection, and the supporting piece is provided with an elastic clamping piece, and the inner wall of the flange of the bottle body is provided with a clamping groove corresponding to the elastic clamping piece.

[0011] Preferably, the upper end of the supporting piece has a cross-sectional area larger than that of the lower end of the supporting piece, and the upper end of the supporting piece is provided with a positioning groove for clamping the supporting piece.

[0012] Preferably, the lower end surface of the supporting piece is provided with an elastic buffer layer.

[0013] Preferably, the inner wall of the bottle body is provided with a plurality of turbulence protrusions for breaking the laminar flow state of the culture solution when the bottle body is shaken, so as to promote the formation of turbulent flow.

[0014] The utility model has the beneficial effect that: through dynamic support spare, the bottle body is placed on the desktop, makes the bottle body can be more smoothly rolling on the desktop, the operator only needs to push the bottle body with light force to shake as the fulcrum of dynamic support spare, compared with the traditional plane bottom culture bottle needs to suspend the whole process and shakes with great force, the operation difficulty is reduced, the hand sore distress is alleviated, the operation failure probability caused by hand sore is reduced, and the shaking uniformity of the culture solution in the bottle body is better, and the cell adhesion efficiency is improved. BRIEF DESCRIPTION OF DRAWINGS

[0015] In order to more clearly illustrate the technical scheme in the embodiment of the utility model or prior art, the following will briefly introduce the drawings needed to be used in the embodiment or prior art description, and obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can be obtained according to these drawings without creative labor.

[0016] Figure 1 The prior art structure schematic diagram provided by the utility model is shown in the drawings.

[0017] Figure 2 The first embodiment structure schematic diagram provided by the utility model is shown in the drawings.

[0018] Figure 3 The second embodiment structure schematic diagram provided by the utility model is shown in the drawings.

[0019] Figure 4 The structure schematic diagram when the supporting piece and the bottle body are separated in the utility model is shown in the drawings.

[0020] Figure 5 The structure schematic diagram when the bottle body is stacked in the utility model is shown in the drawings.

[0021] Figure 6 The cross-sectional structure schematic diagram of the bottle body in the utility model is shown in the drawings.

[0022] Figure 7 Figure is a structure diagram when the stabilizing piece is raised in the utility model.

[0023] In the figure, 1, bottle body; 11, bottle body; 12, cap; 2, stabilizing piece; 3, rotating piece; 4, ball; 5, supporting piece; 51, elastic clamping piece; 52, clamping groove; 6, positioning groove; 7, elastic buffer layer; 8, spoiler. DETAILED DESCRIPTION

[0024] In order to make the technical means, creative features, purposes and effects realized by the utility model easy to understand, the utility model is further described below in combination with specific drawings.

[0025] Referring to Figure 1 As shown in the prior art structure diagram provided by the utility model, a cell culture bottle is attached to a wall, which comprises a bottle body 11 and a cap 12 arranged on the bottle body 11, wherein when the bottle body 11 is placed on a desktop or other bearing surface, it is in contact with the desktop through a flat side, which can improve the stability during placement, but when the culture solution in the bottle body 1 needs to be shaken to increase the cell attachment effect, the bottle body 1 needs to be held by hand and then shaken by repeatedly turning the wrist after being separated from the desktop, but under the condition of long-time work or a large number of experimental operation steps, hand cramps and other phenomena may occur, increasing the risk of the bottle body 1 slipping out of the hand, and once the bottle body 1 falls and breaks, not only will the culture solution be spilled and contaminated, but also safety hazards will be caused, therefore the following improvements are made in the present scheme, a dynamic support is arranged on the bottom of the bottle body 1, i.e. the surface in contact with the desktop, when the bottle body 1 is driven by an external force, the dynamic support drives the bottle body 1 to shake, realizing the uniform and efficient mixing state of the culture solution in the bottle body 1, so that the personnel only need to gently push the bottle body 1 to shake around the dynamic support as the fulcrum, reducing the occurrence of hand cramps, which will be described in detail below in combination with two embodiments:

[0026] Referring to Figure 2 As shown in the first embodiment provided by the utility model, the dynamic support is a rotating piece 3 integrally connected to the bottle body 1, the radial section of the rotating piece 3 is fan-shaped, when the bottle body 1 is placed on the desktop, the arc surface of the rotating piece 3 is in contact with the desktop, and when the bottle body 1 is shaken, only one end of the bottle body 1 needs to be held and then reciprocally swung around the axis of the rotating piece 3, compared with the traditional culture bottle with a flat bottom which needs to be held in the air and shaken greatly, the operating arm is significantly shortened, which is more labor-saving, when the bottle body 1 swings around the axis of the rotating piece 3, the culture solution forms an efficient flow pattern in the bottle body 1 under the action of centrifugal force and inertia, which can effectively break the laminar flow state under the traditional shaking mode, promoting the more sufficient contact between the nutrients, gas and cells in the culture solution.

[0027] Referring to Figure 3 As shown in the second embodiment, the dynamic support is a ball 4 rotatably connected to the bottle body 1. The operator holds the bottle body 1 and gently pushes the bottle body 1. The ball 4 at the bottom of the bottle body 1 rolls on the supporting surface such as a table, driving the bottle body 1 to shake. Since the ball 4 and the table are in point contact, the rolling friction is very small. The experimenter only needs to exert a small force to easily achieve multi-angle and multi-direction shaking of the bottle body 1. The shaking frequency and amplitude can be flexibly adjusted as needed. Like the first embodiment, the ball 4 and the rotating part 3 can be shaken while the bottle body 1 is in contact with the table. Even if the hand slips and falls, it will not cause the bottle body 1 to directly hit the table and cause damage. However, the rotating part 3 in the first embodiment is reciprocally swung around the axis of the rotating part 3. The swinging direction is relatively single, mainly arc swinging around the axis. This swinging method is easy to control, and the operator can accurately control the shaking frequency and amplitude to achieve stable shaking operation. In the second embodiment, the ball 4 can achieve multi-angle and multi-direction shaking, and the operation is more flexible. However, the ball 4 is a separate component that needs to be installed on a specific mounting seat. The structure is relatively complex, and the design and manufacturing precision of the mounting seat are relatively high.

[0028] Referring to Figures 3-4 Further, in order to make the bottle body 1 more stable during non-shaking operation, a stabilizing part 2 is provided on the bottle body 1. The dynamic support is lifted to achieve stable placement of the bottle body 1. Specifically, the stabilizing part 2 includes a supporting part 5 that is slidably connected to the flanges of the bottle body 1. The supporting part 5 is provided with an elastic clamping part 51. The inner wall of the flange of the bottle body 1 is provided with a clamping groove 52 corresponding to the elastic clamping part 51. By pressing the supporting part 5 downward, the elastic clamping part 51 enters the clamping groove 52. At this time, the supporting part 5 is fixed to the flange of the bottle body 1, and the bottom end of the supporting part 5 is lower than the lowest point of the dynamic support. Thus, the bottle body 1 and the dynamic support can be lifted. At this time, the contact between the dynamic support and the table is changed to the contact between the stabilizing part 2 and the table, so that the bottle body 1 can be stably placed on the table. When shaking the bottle body 1 is needed, the dynamic support directly contacts the table by sliding the supporting part 5 upward. The elastic clamping part 51 can be a reed or an elastic protrusion that can deform under pressure and restore deformation when the pressure is removed.

[0029] Referring to Figure 5As shown, further, the upper end cross-sectional area of the supporting piece 5 is greater than the lower end cross-sectional area of the supporting piece 5, and the upper end of the supporting piece 5 is provided with a positioning groove 6 for clamping the supporting piece 5, in the cell culture experiment or production process, a large number of cells usually need to be cultured, and a large number of bottle bodies 1 will be used, when a plurality of bottle bodies 1 are stacked, the elastic clamping piece 51 is clamped into the clamping groove 52, and then the supporting piece 5 on the upper bottle body 1 is clamped into the positioning groove 6 on the lower bottle body 1, so that the stacking of the plurality of bottle bodies 1 is realized.

[0030] Referring to Figure 2 As shown, further, the lower end surface of the supporting piece 5 is provided with an elastic buffer layer 7, which can be made of rubber, silicone or the like, so as to increase the adhesion and friction with the placement plane such as a table top, and ensure stable placement of the bottle body 1.

[0031] Among them, a plurality of turbulence protrusions 8 are arranged on the inner wall of the bottle body 1, which are used to destroy the laminar flow state of the culture solution when the bottle body 1 shakes, so as to form turbulent flow, so that the cells can be more quickly and uniformly dispersed in the culture solution, thereby promoting the cell adhesion effect.

[0032] The basic principle and main features of the present application and the advantages of the present application are shown and described above. It should be understood by those skilled in the art that the present application is not limited by the above embodiments, and the above embodiments and descriptions in the specification are only to illustrate the principle of the present application. Without departing from the spirit and scope of the present application, the present application can also have various changes and improvements, and these changes and improvements all fall within the scope of the present application. The scope of protection of the present application is defined by the appended claims and their equivalents.

Claims

1. An adherent cell culture flask, characterized in that, The utility model relates to a bottle body (1) for containing culture solution, a dynamic support provided at the bottom of the bottle body (1) for contacting a table top, and a stabilizing member (2) provided on the bottle body (1) for lifting the dynamic support to stably place the bottle body (1) in a non-operation state. The dynamic support is a rotating member (3) integrally formed on the bottle body (1), and the radial cross section of the rotating member (3) is fan-shaped. The dynamic support is a ball (4) rotatably connected to the bottle body (1). The stabilizing member (2) includes a supporting member (5) dampedly and slidably connected to the two side flanges of the bottle body (1), and the supporting member (5) is provided with an elastic clamping member (51), and the inner wall of the flange of the bottle body (1) is provided with a clamping groove (52) corresponding to the elastic clamping member (51).

2. The culture flask of claim 1, wherein The upper end of the supporting member (5) has a larger cross-sectional area than the lower end of the supporting member (5), and the upper end of the supporting member (5) is provided with a positioning groove (6) for clamping the supporting member (5).

3. The cell culture flask of claim 1, wherein The lower end of the supporting member (5) is provided with an elastic buffer layer (7).

4. The cell culture flask of claim 1, wherein The inner wall of the bottle body (1) is provided with a plurality of turbulence protrusions (8) for breaking the laminar flow state of the culture solution when the bottle body (1) is shaken to form a turbulent flow.

5. The culture flask of claim 4, wherein the surface is a hydrophilic surface. ​ 6. The culture flask of claim 4, wherein the surface is a hydrophilic surface. ​ 7. The cell culture flask of claim 1, wherein ​

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