In-vitro culture device for immature eggs

Abstract

The utility model discloses an in-vitro culture device for immature eggs. The device comprises a first culture dish, the upper end of the first culture dish is slidably connected with a second culture dish, the upper end of the second culture dish is slidably connected with a top cover, the peripheral side surface of the second culture dish is fixedly connected with a limiting ring, the bottom surface of the inner wall of the first culture dish is provided with a plurality of groups of culture grooves, the bottom surface of the inner wall of the first culture dish is provided with an information exchange groove for communicating two groups of adjacent culture grooves, the bottom of the inner wall of the second culture dish is provided with a substance exchange hole in a penetrating manner, the inner wall of the substance exchange hole is provided with a side groove, and the peripheral side surface of the inner wall of the second culture dish is provided with a through hole; the oocytes can be conveniently found, positioned and observed through the information communication groove, meanwhile, the upper end of the information communication groove is open and can be conveniently cleaned, and nutrient substances can still penetrate through the side groove under the condition that the substance communication hole is blocked by the cells through the side groove, so that the oocytes can stably develop.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of assisted reproductive technology, concretely to an in vitro culture device of immature ovum. BACKGROUND

[0002] The assisted reproductive technology is the abbreviation of human assisted reproductive technology, refers to the technology that makes infertile couple pregnancy by medical assistance, including artificial insemination and in vitro fertilization-embryo transfer and its derivative technology, artificial insemination refers to a method that injects semen into female body by artificial method to replace sexual intercourse to make it pregnant. According to the different sources of semen, it is divided into husband semen artificial insemination and donor artificial insemination, in vitro fertilization-embryo transfer technology and its various derivative technologies refer to the technology that takes out ovum from female body, after culture in a vessel, adds the sperm treated by technology, after the ovum is fertilized, continues to culture, when early embryo is formed, is transferred to the uterus again and is implanted, develops into fetus until delivery. In vitro fertilization-embryo transfer and its derivative technology mainly include in vitro fertilization-embryo transfer, gamete or zygote intrafallopian transfer, intracytoplasmic sperm injection, embryo freezing, preimplantation genetic diagnosis and the like.

[0003] Through the search, the utility model patent with patent No. CN221720830U discloses "an oocyte in vitro maturation culture mechanism", the oocyte in vitro maturation culture mechanism is divided into upper and lower two-layer structures, can be combined by the different use purposes, optimizes the coculture process, the first culture dish is designed with culture tank and information exchange channel to facilitate the search, positioning observation of oocyte and information exchange between adjacent two oocytes, makes the culture of oocyte safer, and at the same time, the upper follicular fluid exosome can continuously transport nutrient substances and signal molecules to oocyte through the material exchange hole, which is beneficial to the in vitro maturation of oocyte and improves the embryo development potential, the top cover is fixed through the limiting assembly, the limiting assembly is simple to operate, the top cover is convenient to open, and the fixing effect is stable, more practical.

[0004] The material exchange hole in the device is a circular hole, which can transport nutrient substances and isolate cells, but the circular material exchange hole is easily blocked by cells, which leads to the failure of normal nutrient substance transportation and poor stability. UTILITY MODEL CONTENT

[0005] The utility model aims at providing an in vitro culture device of immature ovum to solve the problems in the above background technology.

[0006] In order to achieve the above object, the utility model provides the following technical scheme: an in vitro culture device of immature egg, include: first culture dish, the first culture dish upper end slidingly connected with second culture dish, the second culture dish upper end slidingly connected with top cap, the second culture dish circumferential surface is fixedly connected with the limiting ring, the first culture dish inner wall bottom is equipped with the culture tank, and culture tank is equipped with multiple groups, the first culture dish inner wall bottom is equipped with the information exchange groove that two groups of adjacent culture tank are connected, the second culture dish inner wall bottom is equipped with the material exchange hole, and material exchange hole is equipped with multiple groups, the material exchange hole inner wall is equipped with the side groove, and side groove is equipped with multiple groups, the second culture dish inner wall circumferential surface is equipped with the through -hole, and through -hole is equipped with multiple groups.

[0007] Further, the first culture dish upper end is equipped with the positioning groove, and the positioning groove is equipped with four groups.

[0008] Further, the limiting ring bottom is fixedly connected with the positioning block, and the positioning block is provided with four groups.

[0009] Further, the first culture dish circumferential surface is equipped with the first sliding slot, and the first sliding slot is equipped with two groups, and the first sliding slot is internally provided with a limiting component.

[0010] Further, the limiting component includes the limiting block slidingly connected in the first sliding slot, includes the limiting plate slidingly connected in the first sliding slot, and the limiting plate is fixedly connected with the limiting block.

[0011] Further, the limiting component further includes the spring fixedly connected in the first sliding slot, and the spring is fixedly connected with the limiting plate.

[0012] Further, the top cap circumferential surface is equipped with the second sliding slot, and the second sliding slot is equipped with two groups, and the second sliding slot is internally provided with a squeezing mechanism.

[0013] Further, the squeezing mechanism includes the pressing block slidingly connected in the second sliding slot, includes the squeezing plate slidingly connected in the second sliding slot, and the squeezing plate is fixedly connected with the pressing block.

[0014] Compared with the prior art, the utility model has the advantages that:

[0015] The utility model discloses through information exchange groove can conveniently find, positioning observation of oocyte, and the upper end of information exchange groove is open, can be convenient for washing, through the setting of side groove, can still pass through the inside of side groove with the material exchange hole of cell blocking, can make oocyte stable development. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is the front view structure schematic diagram in an embodiment of the utility model.

[0017] Figure 2 For Figure 1 The structure diagram of the first culture dish and its internal components is shown in the figure.

[0018] Figure 3 For Figure 1 The local enlarged view of the A position is shown in the figure.

[0019] Figure 4 For Figure 1 The local enlarged view of the B position is shown in the figure.

[0020] Figure 5 For Figure 1 The structure diagram of the extrusion mechanism and Figure 2 The structure diagram of the limiting component is shown in the figure.

[0021] Reference signs: 1, first culture dish; 2, second culture dish; 3, top cover; 4, limiting ring; 5, culture groove; 6, information exchange groove; 7, material exchange hole; 8, side groove; 9, through hole; 10, positioning groove; 11, positioning block; 12, first sliding groove; 13, limiting component; 131, limiting block; 132, limiting plate; 133, spring; 14, second sliding groove; 15, extrusion mechanism; 151, pressing block; 152, extrusion plate. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0023] Please refer to Figures 1-5 , wherein Figure 1 The front view structure diagram in an embodiment of the present application is shown in the figure. Figure 2 For Figure 1 The structure diagram of the first culture dish and its internal components is shown in the figure. Figure 3 For Figure 1 The local enlarged view of the A position is shown in the figure. Figure 4 For Figure 1 The local enlarged view of the B position is shown in the figure. Figure 5 For Figure 1 The structure diagram of the extrusion mechanism and Figure 2The structure diagram of the middle limiting assembly, an in vitro culture device for immature eggs, comprising: a first culture dish 1, a second culture dish 2 is slidably connected to the upper end of the first culture dish 1, a top cover 3 is slidably connected to the upper end of the second culture dish 2, the top cover 3 is a hollow cylinder with a missing bottom surface, a limiting ring 4 is fixedly connected to the lateral surface of the second culture dish 2, the outer diameter of the limiting ring 4 is the same as the outer diameter of the first culture dish 1, the second culture dish 2 can be limited to the upper position inside the first culture dish 1 through the limiting ring 4, a culture groove 5 is formed in the inner wall bottom surface of the first culture dish 1, and a plurality of culture grooves 5 are formed, an information exchange groove 6 is formed in the inner wall bottom surface of the first culture dish 1, which connects two adjacent culture grooves 5, the width of the information exchange groove 6 can allow nutrients to pass through, but not cells, a plurality of material exchange holes 7 are formed in the inner wall bottom of the second culture dish 2, the hole diameter of the material exchange hole 7 can allow nutrients to flow through, but not cells, a plurality of side grooves 8 are formed in the inner wall of the material exchange hole 7, the inside of the side groove 8 can allow nutrients to flow through, a plurality of through holes 9 are formed in the lateral surface of the inner wall of the second culture dish 2, and the gas inside the first culture dish 1 and the second culture dish 2 can flow through the through holes 9.

[0024] A positioning groove 10 is formed in the upper end of the first culture dish 1, and four positioning grooves 10 are formed.

[0025] The bottom of the limiting ring 4 is fixedly connected with a positioning block 11, and the positioning block 11 is provided with four groups, the positioning groove 10 is matched with the positioning block 11, the limiting ring 4 can be installed on the upper end of the first culture dish 1 through the positioning groove 10 and the positioning block 11, and the material exchange hole 7 at the bottom of the second culture dish 2 is aligned with the culture groove 5 at the bottom of the first culture dish 1.

[0026] A first sliding groove 12 is formed in the lateral surface of the first culture dish 1, and two first sliding grooves 12 are formed, a limiting assembly 13 is arranged in the first sliding groove 12, the two first sliding grooves 12 are symmetrically arranged on the lateral surface of the first culture dish 1, the inner wall of the inner position of the first sliding groove 12 is matched with the limiting plate 132, and the inner wall of the opening position is matched with the limiting block 131.

[0027] The limiting assembly 13 comprises a limiting block 131 slidably connected in the first sliding groove 12, a limiting plate 132 slidably connected in the first sliding groove 12, and the limiting plate 132 is fixedly connected with the limiting block 131.

[0028] The limiting assembly 13 further comprises a spring 133 fixedly connected in the first sliding groove 12, and the spring 133 is fixedly connected with the limiting plate 132.

[0029] The second sliding groove 14 is provided on the periphery of the top cover 3, and two groups of the second sliding groove 14 are provided; the second sliding groove 14 is internally provided with an extrusion mechanism 15; the two groups of the second sliding groove 14 are symmetrically arranged on the periphery of the top cover 3; the second sliding groove 14 is provided in a penetrating manner and is in communication with the inside of the top cover 3; the inner wall of the second sliding groove 14 is matched with the extrusion plate 152 at the middle part; the outer end inner wall is matched with the pressing block 151; and the inner end inner wall is matched with the limiting block 131.

[0030] The extrusion mechanism 15 comprises a pressing block 151 which is slidingly connected in the second sliding groove 14, and an extrusion plate 152 which is slidingly connected in the second sliding groove 14, and the extrusion plate 152 is fixedly connected with the pressing block 151.

[0031] In summary, the in vitro culture device for immature eggs provided by the utility model can conveniently find, position and observe the oocytes through the information exchange groove 6, the upper end of the information exchange groove 6 is open, so that the information exchange groove 6 is convenient to clean, the opening of the side groove 8 can make the cell block the material exchange hole 7, and the nutrient substance can still pass through the inside of the side groove 8, so that the oocyte can stably develop;

[0032] The inner end of the second sliding groove 14 is vertically aligned with the first sliding groove 12, then the top cover 3 is covered on the upper end of the first culture dish 1, the bottom edge of the top cover 3 extrudes the limiting block 131, at this time, the limiting block 131 extrudes the spring 133 through the limiting plate 132 and enters the inside of the first sliding groove 12, when the second sliding groove 14 is transversely aligned with the first sliding groove 12, the limiting block 131 is subjected to the elastic force of the spring 133 and enters the inside of the second sliding groove 14, at this time, the top cover 3 can be limited on the upper end of the first culture dish 1 and the second culture dish 2, and the two groups of the pressing block 151 are pinched, so that the pressing block 151 enters the inside of the second sliding groove 14, at this time, the extrusion plate 152 can extrude the limiting block 131 from the inside of the second sliding groove 14, at this time, the top cover 3 can be taken down, and the mechanism can be more convenient for the installation and taking down of the top cover 3.

Claims

1. An apparatus for in vitro culture of immature ova, characterized by comprising: The application relates to a culture dish. The first culture dish (1) is slidably connected with a second culture dish (2) at the upper end, the second culture dish (2) is slidably connected with a top cover (3) at the upper end, the second culture dish (2) is fixedly connected with a limiting ring (4) on the lateral surface, a culture groove (5) is formed in the bottom of the inner wall of the first culture dish (1), and a plurality of groups of culture grooves (5) are formed, an information exchange groove (6) is formed in the bottom of the inner wall of the first culture dish (1) and connects two adjacent culture grooves (5), a plurality of groups of material exchange holes (7) are formed in the bottom of the inner wall of the second culture dish (2), a lateral groove (8) is formed in the inner wall of the material exchange hole (7), and a plurality of groups of lateral grooves (8) are formed, a plurality of groups of through holes (9) are formed in the lateral surface of the inner wall of the second culture dish (2).

2. The apparatus for in vitro culture of immature ova according to claim 1, wherein The first culture dish (1) is slidably connected with a second culture dish (2) at the upper end, the second culture dish (2) is slidably connected with a top cover (3) at the upper end, the second culture dish (2) is fixedly connected with a limiting ring (4) on the lateral surface, a culture groove (5) is formed in the bottom of the inner wall of the first culture dish (1), and a plurality of groups of culture grooves (5) are formed, an information exchange groove (6) is formed in the bottom of the inner wall of the first culture dish (1) and connects two adjacent culture grooves (5), a plurality of groups of material exchange holes (7) are formed in the bottom of the inner wall of the second culture dish (2), a lateral groove (8) is formed in the inner wall of the material exchange hole (7), and a plurality of groups of lateral grooves (8) are formed, a plurality of groups of through holes (9) are formed in the lateral surface of the inner wall of the second culture dish (2).

3. The device for in vitro culture of immature oocytes according to claim 2, characterized in that, The limiting ring (4) is fixedly connected with a positioning block (11) at the bottom, and the positioning block (11) is provided with four groups.

4. The device for in vitro culture of immature ova according to claim 3, wherein The first culture dish (1) is slidably connected with a second culture dish (2) at the upper end, the second culture dish (2) is slidably connected with a top cover (3) at the upper end, the second culture dish (2) is fixedly connected with a limiting ring (4) on the lateral surface, a culture groove (5) is formed in the bottom of the inner wall of the first culture dish (1), and a plurality of groups of culture grooves (5) are formed, an information exchange groove (6) is formed in the bottom of the inner wall of the first culture dish (1) and connects two adjacent culture grooves (5), a plurality of groups of material exchange holes (7) are formed in the bottom of the inner wall of the second culture dish (2), a lateral groove (8) is formed in the inner wall of the material exchange hole (7), and a plurality of groups of lateral grooves (8) are formed, a plurality of groups of through holes (9) are formed in the lateral surface of the inner wall of the second culture dish (2).

5. The device for in vitro culture of immature ova according to claim 4, wherein The limiting component (13) comprises a limiting block (131) slidably connected in the first sliding groove (12), a limiting plate (132) slidably connected in the first sliding groove (12), and the limiting plate (132) is fixedly connected with the limiting block (131).

6. The device for in vitro culture of immature ova according to claim 5, wherein The limiting component (13) further comprises a spring (133) fixedly connected in the first sliding groove (12), and the spring (133) is fixedly connected with the limiting plate (132).

7. The device for in vitro culture of immature oocytes according to claim 6, characterized in that, The top cover (3) is provided with a second sliding groove (14) on the lateral surface, and the second sliding groove (14) is provided with two groups of extrusion mechanisms (15).

8. The device for in vitro culture of immature oocytes according to claim 7, characterized in that, The extrusion mechanism (15) comprises a pressing block (151) slidably connected in the second sliding groove (14), an extrusion plate (152) slidably connected in the second sliding groove (14), and the extrusion plate (152) is fixedly connected with the pressing block (151).

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