Cell scanning image analyzer

Abstract

The embodiment of the utility model provides a cell scanning image analysis device, which comprises a rotary spectroscope used for reflecting and transmitting fluorescence signals with different wavelengths; the high-precision stepping motor is used for driving the rotary spectroscope to be switched among different positions; the fixing frame is used for supporting and fixing the rotary spectroscope and the high-precision stepping motor; the machine base is used for providing support; a lens barrel forming a channel for observing a cell image; the lens group is mounted between the rotary spectroscope and the sample detection platform; and the optical filter frame is fixed above the lens group and is used for mounting and fixing a plurality of optical filters so as to separate fluorescence signals with different wavelengths. According to the scheme of the embodiment of the invention, the problem that the superposition error of multicolor fluorescence signals is increased due to the fact that the switching speed between different channels needs extremely high stability in multi-channel fluorescence imaging and a non-synchronous phenomenon possibly occurs when a mechanical transmission part runs at a high speed can be solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of bioengineering, in particular to a cell scanning image analysis device. BACKGROUND

[0002] The cell scanning image analysis device is an instrument for high-precision imaging and analyzing cell structure and function, which usually adopts multi-channel fluorescence imaging technology to capture different biomarkers. However, there is a technical challenge in the practical application of this device: the switching speed between different channels needs to be extremely stable during multi-channel fluorescence imaging. If the mechanical transmission components are out of synchronization when running at high speed, it will cause a large error when multi-color fluorescence signals are superimposed, affecting the accuracy and reliability of imaging. SUMMARY

[0003] Therefore, the present application provides a cell scanning image analysis device to at least partially solve the problems in the prior art.

[0004] The cell scanning image analysis device of the present application comprises:

[0005] A rotating spectroscope for reflecting and transmitting fluorescence signals of different wavelengths;

[0006] A high-precision stepper motor for driving the rotating spectroscope to switch between different positions;

[0007] A fixed frame for supporting and fixing the rotating spectroscope and the high-precision stepper motor;

[0008] A machine base for providing support;

[0009] A lens barrel forming a channel for observing cell images;

[0010] A lens group installed between the rotating spectroscope and the sample detection platform;

[0011] A filter holder fixed above the lens group for mounting and fixing a plurality of filters to separate fluorescence signals of different wavelengths;

[0012] The rotating spectroscope and the high-precision stepper motor are connected through a shaft coupling to realize synchronous motion; and

[0013] The edge of the rotating spectroscope is provided with a positioning hole matched with the shaft end of the high-precision stepper motor.

[0014] Preferably, the rotating spectroscope has a multi-layer coating structure.

[0015] Preferably, the diameter of the rotating spectroscope ranges from 50mm to 100mm, and the thickness ranges from 1mm to 3mm.

[0016] Preferably, the high-precision stepper motor is a brushless DC motor.

[0017] The high-precision stepper motor is internally integrated with an optical encoder for real-time monitoring of the position and speed of the rotor thereof; and

[0018] The resolution of the optical encoder is not less than 1 micrometer.

[0019] Preferably, the fixed frame is internally provided with a plurality of shock-absorbing structures.

[0020] Preferably, the shock-absorbing structures are composed of a plurality of independent spring buffering units, which are uniformly distributed on the support points of the fixed frame.

[0021] Preferably, the lens group comprises built-in high-precision lens elements, which are finely adjusted by a precision adjustment mechanism.

[0022] The precision adjustment mechanism comprises a micro motor, a screw rod, an adjusting block, a guide rod, a guide block and a position sensor. The micro motor drives the screw rod to rotate, so that the adjusting block drives the high-precision lens elements to move. The guide rod and the guide block limit and guide the movement of the high-precision lens elements. Meanwhile, the position sensor monitors the position of the high-precision lens elements in real time, so as to correct the relative position of the lens group.

[0023] Preferably, the filter holder is designed in a modular manner, each filter can be independently assembled, disassembled and replaced, and is provided with a locking mechanism.

[0024] Preferably, the locking mechanism is composed of a pair of first and second magnetic rings with opposite magnetic properties, and the first and second magnetic rings can adsorb the filter.

[0025] The cell scanning image analysis device provided by the embodiment of the present disclosure comprises: a rotating spectroscope for reflecting and transmitting fluorescent signals of different wavelengths; a high-precision stepping motor for driving the rotating spectroscope to switch between different positions; a fixing frame for supporting and fixing the rotating spectroscope and the high-precision stepping motor; a base for providing support; a lens barrel forming a channel for observing cell images; a lens group installed between the rotating spectroscope and a sample detection platform; and a filter holder fixed above the lens group for installing and fixing a plurality of filters to separate fluorescent signals of different wavelengths; wherein the rotating spectroscope and the high-precision stepping motor are connected through a shaft coupling to realize synchronous movement, and the edge of the rotating spectroscope is provided with a positioning hole matched with the shaft end of the high-precision stepping motor. Through the scheme of the embodiment of the present disclosure, the problem of increased multi-color fluorescent signal superposition error caused by the need for extremely high stability of switching speed between different channels in multi-channel fluorescence imaging and the possible non-synchronous phenomenon of mechanical transmission components during high-speed operation can be solved. BRIEF DESCRIPTION OF DRAWINGS

[0026] In order to more clearly illustrate the technical solutions of the exemplary embodiments of the present disclosure, the following will briefly introduce the drawings needed in the embodiments. It should be understood that the following drawings only show some embodiments of the present disclosure, and therefore should not be considered as limiting the scope. For those skilled in the art, other related drawings can also be obtained without creative labor.

[0027] Figure 1 is a structural schematic view of the cell scanning image analysis device according to the present disclosure;

[0028] Figure 2 is an exploded schematic view of the connection relationship between the fixing frame, the high-precision stepping motor and the rotating spectroscope in the cell scanning image analysis device according to the present disclosure;

[0029] Figure 3 is a main sectional view of the lens group in the cell scanning image analysis device according to the present disclosure;

[0030] Figure 4 is a structural schematic view of the filter holder in the cell scanning image analysis device according to the present disclosure.

[0031] In the figure: 1, rotating spectroscope; 2, high-precision stepper motor; 3, fixed frame; 4, lens group; 5, filter holder; 6, positioning hole; 7, photoelectric encoder; 8, damping structure; 9, precision adjustment mechanism; 901, micro motor; 902, lead screw; 903, adjusting block; 904, guide rod; 905, guide block; 906, position sensor; 10, locking mechanism; 101, first magnetic ring; 102, second magnetic ring; 11, machine base; 12, lens barrel DETAILED DESCRIPTION

[0032] It should be noted that the relational terms herein such as first and second and the like are used solely to distinguish one from another entity or action without necessarily requiring or implying that there is any such relationship or order between such entities or actions. Also, the terms "comprises", "comprising", or any other variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can also include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element preceded by "comprises... a" does not, without more constraints, foreclose the existence of additional identical elements in the process, method, article, or apparatus that comprises the recited element.

[0033] As shown in Figure 1 The cell scanning image analysis device of the present application includes a rotating spectroscope 1, a high-precision stepper motor 2, a fixed frame 3, a machine base 11, a lens barrel 12, lenses, and a filter holder 5. These components work together to enable the device to efficiently and stably process multi-channel fluorescence imaging.

[0034] The rotating spectroscope 1 is a key component for reflecting and transmitting different wavelength fluorescence signals. It separates different wavelength fluorescence signals through reflection and transmission, providing clear image information for subsequent analysis. The high-precision stepper motor 2 is used to drive the rotating spectroscope 1 to quickly and stably switch between different positions. The motor achieves highly precise position adjustment through a precise control circuit, ensuring consistent switching speed between different channels. The fixed frame 3 is used to support and fix the rotating spectroscope 1 and the high-precision stepper motor 2, ensuring their stability during high-speed operation. The fixed frame 3 is usually made of high-strength materials to withstand the vibration and torque generated by the high-precision stepper motor 2.

[0035] The base 11 is used to support and stabilize the entire device, ensuring the stability and reliability of the overall structure. The base 11 is usually made of cast iron or aluminum alloy, etc., with high rigidity and damping performance. The lens barrel 12 is an important part of the device, which forms a channel for observing cell images, connecting the sample detection platform and other optical components. The lens group 4 is installed between the rotating spectroscope 1 and the sample detection platform, used to collect fluorescent signals from different channels, ensuring the clarity and quality of imaging. The lens group 4 is composed of several high-precision lenses, which are precisely machined and assembled to effectively focus different wavelengths of fluorescent signals. The filter holder 5 is fixed above the lens group 4, used to install and fix multiple filters. These filters can select appropriate wavelengths according to actual needs, thereby separating different wavelengths of fluorescent signals and improving imaging accuracy and resolution.

[0036] In order to achieve the above functions, the rotating spectroscope 1 and the high-precision stepper motor 2 are connected through a precise coupling. This connection not only ensures the precise synchronous movement between the two, but also reduces the error that may occur during the superposition of multi-color fluorescent signals. Specifically, after receiving the pulse signal sent by the control system, the high-precision stepper motor 2 drives the rotating spectroscope 1 to rotate quickly and accurately through the coupling. This linkage mechanism ensures fast and stable switching between different channels, effectively avoiding the problem of increased signal superposition error caused by mechanical asynchronization.

[0037] In summary, the present application effectively solves the technical problems of inconsistent channel switching speed and possible asynchronization of mechanical transmission components during high-speed operation in multi-channel fluorescence imaging by carefully designing the rotating spectroscope 1, high-precision stepper motor 2, fixed frame 3, base 11, lens barrel 12, lens group 4 and filter holder 5, etc. Through precise control and optimization of each component, the separation accuracy of fluorescent signals and the imaging quality are significantly improved, so that the cell scanning image analysis device can play an important role in various application scenarios.

[0038] In one embodiment, the rotating spectroscope 1 of the cell scanning image analysis device of the present application has a multi-layer coating structure. The design of this multi-layer coating aims to improve the reflection and transmission efficiency of the spectroscope for different wavelengths of fluorescent signals. By carefully designing the number of layers and thickness of the coating, the spectroscope can exhibit higher sensitivity and selectivity when processing multi-color fluorescent signals, thereby reducing mutual interference and superposition error between fluorescent signals. In addition, this multi-layer coating structure can also ensure that the spectroscope maintains stable optical properties during rotation, providing consistent optical performance even at high rotation speeds.

[0039] In one embodiment, the rotating spectroscope 1 is composed of multiple layers of optical material, each layer is precisely calculated and deposited to ensure the expected reflection and transmission behavior of specific wavelengths of light at its interface. To achieve this structure, the spectroscope usually needs to be coated in a vacuum environment through ion sputtering or evaporation deposition technology. This can ensure the uniform thickness of each layer, so as to achieve the best optical effect. For example, a multilayer structure can be formed by alternately depositing high and low refractive index materials on the surface of the substrate, and the thickness and sequence of the specific layers are adjusted according to the required wavelength range.

[0040] Reference Figure 2 In one embodiment, the cell scanning image analysis device of the present application includes a rotating spectroscope 1 and a high-precision stepper motor 2. The edge of the rotating spectroscope 1 is provided with a positioning hole 6, which is used to cooperate with the shaft end of the high-precision stepper motor 2, to ensure that the spectroscope can be accurately aligned and stably installed on the motor shaft. This design not only ensures the stability of the spectroscope during high-speed rotation, but also effectively avoids performance problems caused by asynchronous phenomena. In this way, it can ensure that the entire device operates more reliably and efficiently.

[0041] Specifically, the specific position of the positioning hole 6 is located at the edge of the rotating spectroscope 1, and its shape and size are matched with the shaft end of the high-precision stepper motor 2. During installation, the high-precision stepper motor 2 is first fixed on the device base, and then the positioning hole 6 of the rotating spectroscope 1 is aligned with the shaft end of the motor and installed, to ensure accurate alignment and stable connection between the two. For example, screws or buckles and other fixing elements can be used to further reinforce the installation, to ensure that the spectroscope does not displace during long-term high-speed operation.

[0042] In one embodiment, the rotating spectroscope 1 of the cell scanning image analysis device of the present application has a diameter ranging from 50mm to 100mm and a thickness ranging from 1mm to 3mm. The rotating spectroscope 1 is made of optical glass material with high temperature resistance and high transparency, to ensure excellent optical performance and long-term stability during multi-channel switching. The size and material selection of the spectroscope not only ensure that it can work stably under different light conditions, but also can withstand mechanical stress caused by frequent rotation.

[0043] For example, the rotating spectroscope 1 is usually installed on the central axis of the device and is precisely controlled by motor driving. The motor and the spectroscope are connected through a high-precision coupling, to ensure the stability and accuracy of rotation. The optical performance of the spectroscope is further optimized by coating the surface of the spectroscope, to improve the light transmittance and reduce the reflection loss. In addition, the device is designed with a heat dissipation system to ensure that the spectroscope does not affect the optical performance due to temperature rise during long-term operation. Specifically, precise measuring tools are required during the installation and adjustment process of the spectroscope, to ensure that its position and angle meet the design requirements.

[0044] In one embodiment, the cell scanning image analysis device of the present application employs a high-precision stepper motor 2, specifically a brushless DC motor, which can adapt to high-temperature and high-load conditions, ensuring stable driving force. This motor can maintain high-performance operation in complex environments, effectively reducing signal superposition errors caused by motor performance fluctuations. In addition, the high-precision stepper motor 2 integrates an optical encoder 7 for real-time monitoring of rotor position and speed, ensuring the accuracy and consistency of each channel switching time.

[0045] Specifically, the resolution of the optical encoder 7 is not less than 1 micrometer, which can provide sub-micrometer positioning accuracy during high-speed switching, further reducing multi-color fluorescent signal superposition errors caused by asynchronous phenomena. The optical encoder 7 is installed inside the high-precision stepper motor 2 and synchronously operates with the rotor part through precise mechanical connection, ensuring the real-time and accuracy of the feedback signal.

[0046] For example, the stator and rotor parts of the high-precision stepper motor 2 are designed as a tightly fitted structure, with the stator fixed to the main frame of the device and the rotor connected to the scanning mechanism that needs to be driven. The optical encoder 7 is coaxially installed with the rotor and transmits monitoring information in real time to the control unit through data lines for precise control and correction. In this way, the entire device can achieve high precision and high consistency during multiple channel switching processes.

[0047] In one embodiment, the cell scanning image analysis device of the present application, the fixed frame 3 is made of high-strength aluminum alloy material, which can ensure the mechanical strength and stability of the fixed frame 3 during long-time high-speed operation. In order to further improve the overall performance and service life of the device, the fixed frame 3 is provided with multiple damping structures 8 (see Figure 2 ) inside, which can effectively absorb and disperse the vibrations generated during high-speed operation. In this way, the device can reduce mechanical wear and asynchronous phenomena caused by vibration, thereby improving the running stability and reliability of the overall device.

[0048] Specifically, the damping structure 8 is composed of multiple independent spring buffer units, which are distributed on the key support points of the fixed frame 3. Each spring buffer unit is composed of springs and other damping elements, which can adjust the buffering effect according to the actual stress condition, ensuring that each part is evenly stressed during high-speed operation. Through this design, the entire device can effectively disperse stress during operation, avoiding the problem of asynchronous caused by local overload, thereby improving the working efficiency and service life of the device.

[0049] For example, the key support points of the fixed frame 3 include the bottom contact points and the side wall support points, and one or more month spring buffer units are installed at each support point. The installation position of each spring buffer unit is accurately calculated and experimentally verified to ensure that the vibration is maximally absorbed and dispersed when the device is running at high speed. In this way, not only the stability of the fixed frame 3 itself is improved, but also other key components are indirectly protected, prolonging the overall life of the device.

[0050] In one embodiment, referring to Figure 3 The lens group 4 of the cell scanning image analysis device of the present application includes built-in high-precision lens elements that are fine-tuned by a precision adjustment mechanism to ensure the focusing quality and consistency of different channel fluorescence signals. The high-precision lens elements are installed inside the lens group 4 and can move within a predetermined range inside the lens group 4 to adjust the focal length and alignment accuracy. The precision adjustment mechanism includes multiple components such as a micro motor 901, a lead screw 902, an adjustment block 903, a guide rod 904, a guide block 905, and a position sensor 906, which work together to accurately control the displacement of the high-precision lens elements.

[0051] In one embodiment, the micro motor 901 is installed at one end of the lens group 4 and connected to the lead screw 902. By driving the lead screw 902 to rotate, the lead screw 902 engages with the adjustment block 903, thereby converting the rotary motion of the motor into the linear motion of the adjustment block 903. The adjustment block 903 is fixed to the high-precision lens element, and as the adjustment block 903 moves, the high-precision lens element synchronously displaces to achieve precise adjustment of the focal length. The guide rod 904 is installed on both sides of the lens group 4 and cooperates with the guide block 905 to limit and guide the movement of the adjustment block 903 and the high-precision lens element, ensuring smooth movement along the preset path. The position sensor 906 is installed inside the lens group 4 to monitor the position of the high-precision lens element in real time and feed back the data to the control system for correcting the relative position of the lens group 4 to ensure that the fluorescence signals of each channel remain clear and sharp during high-speed switching.

[0052] For example, through the above-mentioned precision adjustment mechanism, the focal length of the lens group 4 can be quickly adjusted during high-throughput fluorescence imaging to adapt to the changes in the thickness of different cell samples and the intensity of fluorescence signals, improving the accuracy and efficiency of image analysis.

[0053] Referring to Figure 4In one embodiment, the filter holder 5 of the cell scanning image analysis device of the present application adopts a modular design, allowing each filter to be independently installed and replaced. Specifically, each filter on the filter holder 5 is fixed by a locking mechanism 10, ensuring that there is no loosening or deviation during high-frequency switching. The locking mechanism 10 includes a pair of first and second magnetic rings 101 and 102 with opposite magnetic properties, and the secure installation of the filter is achieved through the magnetic attraction between the two.

[0054] For example, the first and second magnetic rings 101 and 102 are respectively arranged at the edge positions of the filter holder 5 and the filter, and when the filter is installed in place, the first and second magnetic rings 101 and 102 are attracted to each other, forming a stable locking effect. In addition, the first and second magnetic rings 101 and 102 have opposite magnetic properties, which can ensure accurate alignment during installation, thereby reducing errors caused by the superposition of multi-color fluorescence signals.

[0055] Specifically, the design of the filter holder 5 allows it to accommodate multiple different sizes of filters, and the installation and replacement of each filter do not require tools, simplifying the operation process. The design of the locking mechanism 10 not only ensures the stability of the filter during high-frequency switching, but also facilitates maintenance and replacement, improving the flexibility and convenience of the overall device.

[0056] In actual operation, when the device is in use, the user first places the cell sample on the sample detection platform. Next, the high-precision stepper motor 2 drives the rotating spectroscope 1 to switch between different positions quickly and stably, which ensures the consistency of switching speed between channels, thereby improving the efficiency of multi-color fluorescence signal acquisition. At the same time, the fixed frame 3 firmly supports the rotating spectroscope 1 and the high-precision stepper motor 2, ensuring their stability during high-speed operation and preventing errors caused by mechanical vibration.

[0057] When the rotating spectroscope 1 switches to different positions, it reflects and transmits different wavelengths of fluorescence signals, which become clear after focusing by the lens group 4. The lens group 4 is installed between the rotating spectroscope 1 and the sample detection platform, ensuring that the fluorescence signals emitted from the cell sample can be accurately transmitted. Subsequently, the multiple filters on the filter holder 5 separate the different wavelengths of fluorescence signals, further improving the purity and resolution of the signals.

[0058] Throughout the whole process, the base 11 provides a solid support, ensuring the stability and reliability of the entire device. In addition, the lens barrel 12 forms a channel for observing the image of the cells, so that the operator can clearly observe the fluorescent image of the cells through the eyepiece or the camera equipment. Finally, the rotating spectroscope 1 and the high-precision stepper motor 2 realize precise synchronous movement through the coupling, effectively reducing the superposition error of the multi-color fluorescent signal, thereby ensuring the accuracy of the final analysis result.

[0059] The above merely describes a specific embodiment of the present application, but the protection scope of the present application is not limited thereto, and any person skilled in the art can easily think of various changes or replacements within the technical range disclosed by the present application, which should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A cellular scanning image analysis apparatus, characterized by comprising: The application relates to a rotating optical splitter (1) for reflecting and transmitting fluorescent signals of different wavelengths, a high-precision stepping motor (2) for driving the rotating optical splitter (1) to switch between different positions, a fixing frame (3) for supporting and fixing the rotating optical splitter (1) and the high-precision stepping motor (2), a base (11) for providing support, a lens barrel (12) forming a channel for observing cell images, a lens group (4) installed between the rotating optical splitter (1) and a sample detection platform, and a filter holder (5) fixed above the lens group (4) and used for mounting and fixing a plurality of filters to separate fluorescent signals of different wavelengths. The rotating optical splitter (1) and the high-precision stepping motor (2) are connected through a shaft coupling to realize synchronous movement. The rotating optical splitter (1) has a multi-layer coating structure. The rotating optical splitter (1) has a diameter ranging from 50 mm to 100 mm and a thickness ranging from 1 mm to 3 mm. The high-precision stepping motor (2) is a brushless direct-current motor. The high-precision stepping motor (2) is internally integrated with a photoelectric encoder (7) for monitoring the position and speed of the rotor in real time. The resolution of the photoelectric encoder (7) is not less than 1 micrometer. The fixing frame (3) is internally provided with a plurality of damping structures (8). The damping structures (8) are composed of a plurality of independent spring buffer units which are uniformly distributed on the support points of the fixing frame (3). The lens group (4) comprises built-in high-precision lens elements which are finely adjusted through a precision adjustment mechanism (9).

2. The cellular scanning image analysis apparatus according to claim 1, characterized by: The precision adjustment mechanism (9) comprises a micro motor (901), a lead screw (902), an adjusting block (903), a guide rod (904), a guide block (905) and a position sensor (906), the micro motor (901) drives the lead screw (902) to rotate to move the adjusting block (903) and the high-precision lens elements, the guide rod (904) and the guide block (905) limit and guide the movement, and the position sensor (906) monitors the position of the high-precision lens elements in real time to correct the relative position of the lens group (4).

3. The cellular scanning image analysis apparatus according to claim 1, characterized by: The filter holder (5) is modularly designed, each filter can be independently mounted, dismounted and replaced, and is provided with a locking mechanism (10).

4. The cellular scanning image analysis apparatus according to claim 1, characterized by: The locking mechanism (10) is composed of a pair of first and second magnetic rings (101) and (102) with opposite magnetic properties, and the first and second magnetic rings (101) and (102) can adsorb the filters. ​ ​ 5. The cellular scanning image analysis apparatus according to claim 1, characterized by: ​ 6. The cellular scanning image analysis apparatus according to claim 5, characterized by: ​ 7. The cellular scanning image analysis apparatus according to claim 1, characterized by: ​ ​ 8. The cellular scanning image analysis apparatus according to claim 1, characterized by: ​ 9. The cellular scanning image analysis apparatus according to claim 8, characterized by: ​

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