Cell culture magnetic field generating device

Abstract

The utility model relates to the technical field of cell culture, and discloses a cell culture magnetic field generating device which comprises an external part and an internal part, the outer part comprises a base plate, an outer sleeve and an inner sleeve, the outer sleeve and the inner sleeve are connected to the base plate, the inner sleeve is located in the outer sleeve, and a cell culture frame is arranged in the inner sleeve; the internal part comprises a coil fixing barrel and a coil wound on the coil fixing barrel, and the coil fixing barrel is arranged on the outer side of the inner sleeve in a sleeving mode; wherein the inner diameter of the outer sleeve is larger than the outer diameter of the inner sleeve, a condensation cooling cabin is formed between the outer sleeve and the inner sleeve, and cooling substances are injected into the condensation cooling cabin. The temperature of the coil in the magnetic field generation process can be effectively reduced, the temperature stability of the coil in the experiment process is ensured, and performance reduction or damage caused by overheating of the coil is avoided.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture technical field, especially a cell culture magnetic field generating device. BACKGROUND

[0002] The research of magnetic field regulating cell function has attracted widespread attention in recent years due to its great application potential in biomedical and bioengineering fields. Magnetic field can affect the physiological activities of cells, including cell proliferation, differentiation, migration and apoptosis, which provides a new idea for cell therapy, tissue engineering and regenerative medicine. Studies have shown that appropriate magnetic field strength and frequency can promote the directional differentiation of stem cells and improve their therapeutic effect. In addition, magnetic field can also affect intracellular signal transduction by regulating the membrane potential and ion channel activity of cells, thereby changing the function of cells. In recent years, with the development of nanotechnology, the application of magnetic nanomaterials has made the research of magnetic field regulating cell function more in-depth, and relevant experimental results have emerged continuously, laying a foundation for future clinical applications. Overall, the research of magnetic field regulating cell function not only enriches our understanding of cell biology, but also provides new strategies for disease treatment and regenerative medicine.

[0003] At present, the magnetic field generating device used in cell experiments mainly includes a solenoid magnetic field generating device, which generates a magnetic field by passing current into the solenoid. However, during the magnetic field generation process, the coil generates heat, which may cause the coil to be damaged. UTILITY MODEL CONTENT

[0004] In order to solve the above technical problems, the present application provides a cell culture magnetic field generating device.

[0005] The present application provides a cell culture magnetic field generating device, comprising:

[0006] The external part includes a bottom disc, an outer sleeve and an inner sleeve connected to the bottom disc, the inner sleeve is located inside the outer sleeve, and the inner sleeve is provided with a cell culture rack inside;

[0007] The internal part includes a coil fixing cylinder and a coil wound on the coil fixing cylinder, and the coil fixing cylinder is sleeved outside the inner sleeve.

[0008] Wherein, the inner diameter of the outer sleeve is greater than the outer diameter of the inner sleeve, a condensation cooling cabin is formed between the outer sleeve and the inner sleeve, and the condensation cooling cabin is used for injecting cooling substances.

[0009] Optionally, the outer sleeve is provided with a wire inlet and a wire outlet respectively at positions close to both ends, and the wire inlet and the wire outlet are respectively provided with sealing plugs.

[0010] Optionally, the cell culture shelf is located in the middle of the inner sleeve, and is in a hollow cross shape, a hollow square shape or a hollow cross shape.

[0011] Optionally, the inner sleeve has an inner diameter of 200-300 mm.

[0012] Optionally, the coil fixing cylinder is detachably connected to the outer side of the inner sleeve, and the inner diameter of the coil fixing cylinder is greater than the outer diameter of the inner sleeve.

[0013] Optionally, the inner part further comprises:

[0014] The limiting ring is movably sleeved on the end of the coil fixing cylinder away from the bottom disc.

[0015] Optionally, the outer sleeve is provided with a water outlet on the side close to the bottom disc.

[0016] Optionally, the coil is covered with a protective layer.

[0017] Optionally, the bottom disc, the outer sleeve, the inner sleeve and the cell culture shelf are integrally formed.

[0018] The technical scheme provided by the embodiment of the application has the following advantages compared with the prior art:

[0019] The cell culture magnetic field generating device provided by the embodiment of the application can generate a magnetic field by passing current through the coil to meet the magnetic field requirement of cell culture. The annular space between the outer sleeve and the inner sleeve serves as a condensation cooling cabin. Before the experiment, appropriate ice water or dry ice is injected into the condensation cooling cabin to ensure that the temperature of the coil is stable during the experiment, and to avoid performance degradation or damage caused by overheating of the coil. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 A structural schematic view of a cell culture magnetic field generating device provided by the embodiment of the application is shown.

[0021] Figure 2 A structural sectional view of a cell culture magnetic field generating device provided by the embodiment of the application is shown.

[0022] Figure 3 A partial structural schematic view of a cell culture magnetic field generating device provided by the embodiment of the application is shown.

[0023] Figure 4 A structural sectional view of a cell culture magnetic field generating device provided by the embodiment of the application is shown.

[0024] Figure 5 A structural sectional view of a cell culture magnetic field generating device provided by the embodiment of the application is shown.

[0025] Figure 6 A partial structure schematic view of a cell culture magnetic field generating device provided by an embodiment of the present disclosure.

[0026] Figure 7 A partial structure sectional view of a cell culture magnetic field generating device provided by an embodiment of the present disclosure.

[0027] Explanation of reference signs:

[0028] 1, outer sleeve; 2, inner sleeve; 3, cell culture rack; 4, coil fixing cylinder; 5, coil; 6, limiting ring; 7, wire inlet; 8, wire outlet; 9, water outlet; 10, bottom plate. DETAILED DESCRIPTION

[0029] In the description of the present application, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the technical scheme of the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0030] In the description of the present application, it should be understood that the terms "connection" should be understood broadly, unless otherwise specified and limited, for example, it can be fixedly connected, or integrally connected, or mechanically connected, or electrically connected, or directly connected, or indirectly connected through an intermediate medium, or connected inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0031] In addition, in the description of the present application, "a plurality of" means two or more than two. The terms "first", "second" are only for the purpose of description, and cannot be understood as implying or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include one or more of the features.

[0032] In addition, in the description of the present application, "a plurality of" means two or more than two. The terms "first", "second" are only for the purpose of description, and cannot be understood as implying or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include one or more of the features.

[0033] The research on the regulation of cell function by magnetic field has attracted extensive attention in recent years due to its great application potential in the fields of biomedical and bioengineering. Magnetic field can affect the physiological activities of cells, including cell proliferation, differentiation, migration and apoptosis, which provides a new idea for cell therapy, tissue engineering and regenerative medicine. Studies have shown that appropriate magnetic field strength and frequency can promote the directional differentiation of stem cells and improve their therapeutic effect. In addition, magnetic field can also affect intracellular signal transduction by regulating the membrane potential and ion channel activity of cells, thereby changing the function of cells. In recent years, with the development of nanotechnology, the application of magnetic nanomaterials has made the research on the regulation of cell function by magnetic field more in-depth, and relevant experimental results have emerged, laying a foundation for future clinical applications. In general, the research on the regulation of cell function by magnetic field not only enriches our understanding of cell biology, but also provides a new strategy for disease treatment and regenerative medicine.

[0034] At present, the magnetic field generating device used in cell experiments mainly includes a solenoid magnetic field generating device, which generates a magnetic field by passing an electric current into the solenoid. However, during the generation of the magnetic field, the coil generates heat, which may cause the coil to be damaged.

[0035] In addition, existing magnetic field generating devices include permanent magnet devices and rotating magnetic field devices. The magnetic field strength of the permanent magnet device is fixed and cannot be adjusted, and it can usually only generate a magnetic field with low intensity. The rotating magnetic field device is complex and has high cost, and has high technical requirements for the operator. In addition, the uniformity and stability of the rotating magnetic field may affect the experimental results. Therefore, the existing magnetic field generating device is difficult to balance the structure cost and the adjustability of the magnetic field.

[0036] Therefore, the embodiment of the present application provides a cell culture magnetic field generating device, which can cool the coil of the magnetic field generating device during the generation of the magnetic field, thereby avoiding damage to the coil due to high temperature. At the same time, by changing the current mode, various magnetic field modes such as alternating magnetic field, pulse magnetic field and constant magnetic field can be generated, and the magnetic field strength can be adjusted to meet the different needs of cell culture.

[0037] At least one embodiment of the present disclosure provides a cell culture magnetic field generating device, comprising:

[0038] The external part comprises a bottom disc, an outer sleeve and an inner sleeve connected to the bottom disc, and the inner sleeve is located inside the outer sleeve. The inner part of the inner sleeve is provided with a cell culture rack;

[0039] The internal part comprises a coil fixing cylinder and a coil wound on the coil fixing cylinder, and the coil fixing cylinder is sleeved outside the inner sleeve;

[0040] The inner diameter of the outer sleeve is greater than the outer diameter of the inner sleeve, and a condensation cooling cabin is formed between the outer sleeve and the inner sleeve. The condensation cooling cabin is used for injecting a cooling substance.

[0041] The cell culture magnetic field generating device provided by the embodiment of the present disclosure can generate a magnetic field to meet the magnetic field requirement of cell culture by passing an electric current to the coil. During operation, ice water or dry ice or other cooling substances are injected into the condensation cooling cabin to effectively reduce the temperature of the coil during the generation of the magnetic field, thereby avoiding damage to the coil due to high temperature.

[0042] The present disclosure will be described below through several specific embodiments. In order to keep the following description of the embodiments of the present disclosure clear and concise, the detailed description of known functions and known components can be omitted. When any component of the embodiments of the present disclosure appears in more than one figure, the component can be denoted by the same reference numeral in each figure.

[0043] As shown in Figure 1 , Figure 2 , Figure 3 The present disclosure provides a cell culture magnetic field generating device, which comprises an external part and an internal part. The external part comprises a bottom disc 10, an outer sleeve 1 connected to the bottom disc 10, and an inner sleeve 2 located inside the outer sleeve 1, and a cell culture rack 3 is arranged inside the inner sleeve 2. The internal part comprises a coil fixing cylinder 4 and a coil 5 wound on the coil fixing cylinder 4, and the coil fixing cylinder 4 is sleeved outside the inner sleeve 2. The inner diameter of the outer sleeve 1 is greater than the outer diameter of the inner sleeve 2, and a condensation cooling cabin is formed between the outer sleeve 1 and the inner sleeve 2, and the condensation cooling cabin is used for injecting cooling substances.

[0044] Specifically, the outer sleeve 1 and the inner sleeve 2 can both be open-ended cylinders, the bottom disc 10 is used to connect and fix the outer sleeve 1 and the inner sleeve 2 and to close one end of the opening, the diameter of the outer sleeve 1 is greater than the diameter of the inner sleeve 2 to form an annular space. The cell culture rack 3 is arranged inside the inner sleeve 2 to facilitate the placement of cell culture dishes. The coil fixing cylinder 4 is sleeved outside the inner sleeve 2, and the coil 5 is wound thereon. An electric current is passed to the coil 5 to generate a magnetic field, so that the cells on the cell culture rack 3 are subjected to a uniform magnetic field, thereby promoting cell growth and differentiation. The annular space between the outer sleeve 1 and the inner sleeve 2 serves as a condensation cooling cabin. Before the experiment, an appropriate amount of ice water or dry ice is injected into the condensation cooling cabin to ensure the stability of the temperature of the coil 5 during the experiment, thereby avoiding performance degradation or damage of the coil 5 due to overheating.

[0045] The inner diameter of the inner sleeve 2 is 200-300 mm, which facilitates the placement of cell culture dishes or plates of different specifications on the cell culture rack 3.

[0046] In an embodiment of the present disclosure, with reference to Figure 5As shown, the outer sleeve 1 is provided with a water outlet 9 near the side close to the base 10. Since the cooling material is directly poured out of the upper opening, it may cause the coil 5 to slide out and is not convenient to operate. Therefore, the cooling material can be discharged through the water outlet 9. At the same time, a one-way valve or a sealing plug can be provided at the water outlet 9 to prevent leakage of the cooling material.

[0047] Further, referring to Figure 3 、 Figure 4 As shown, the outer sleeve 1 is provided with a water outlet 9 near the side close to the base 10. Since the cooling material is directly poured out of the upper opening, it may cause the coil 5 to slide out and is not convenient to operate. Therefore, the cooling material can be discharged through the water outlet 9. At the same time, a one-way valve or a sealing plug can be provided at the water outlet 9 to prevent leakage of the cooling material.

[0048] At the same time, the cell culture rack 3 is located in the middle of the inner sleeve 2 and has a hollow cross-shaped structure. The cell culture rack 3 is used to provide stable support for the cell culture dish, and the hollow cell culture rack 3 facilitates the penetration of the magnetic field, ensures that the cells are subjected to uniform magnetic field, and also makes the cell culture rack 3 more portable.

[0049] In an embodiment of the present application, the coil fixing cylinder 4 is detachably connected to the outer side of the inner sleeve 2, and the inner diameter of the coil fixing cylinder 4 is greater than the outer diameter of the inner sleeve 2. Since the number of turns or the diameter of the coil 5 is different, the magnetic field generated is different. Therefore, according to the experimental requirements, different coil fixing cylinders 4 can be replaced to select appropriate coils 5. The inner diameter of the coil fixing cylinder 4 is greater than the outer diameter of the inner sleeve 2, which ensures convenient installation of the coil 5.

[0050] Further, referring to Figure 6 、 Figure 7 As shown, the inner part further comprises a limiting ring 6. The limiting ring 6 is movably sleeved on the end of the coil fixing cylinder 4 away from the base 10. For example, the limiting ring 6 is provided with an annular groove, the coil fixing cylinder 4 can be inserted into the annular groove, and the limiting ring 6 is threadedly connected with the inner sleeve 2 to realize fixation. After the limiting ring 6 is fixed, it can resist the coil 5 to avoid loosening and displacement of the coil 5, which is conducive to ensuring the uniformity and stability of the generated magnetic field.

[0051] For example, the outer surface of the coil 5 is covered with a protective layer made of high-temperature-resistant, insulating, waterproof and corrosion-resistant material. It can effectively prevent the coil 5 from being damaged and improve the service life of the coil 5.

[0052] In an embodiment of the utility model, the outer sleeve 1, the inner sleeve 2, the cell culture frame 3 and the base plate 10 can be integrally processed from a material with light weight, high temperature resistance, corrosion resistance, high strength and non-magnetic property. The overall structure is ensured to be stable and not interfered by a magnetic field. For example, the material can be ceramic material, polymer-based composite material, carbon fiber composite material, non-magnetic alloy and the like.

[0053] The above disclosed are only several specific embodiments of the utility model, but the utility model embodiments are not limited to this, and any change that can be thought of by any person skilled in the art should fall into the protection scope of the utility model.

Claims

1. A cell culture magnetic field generating device, characterized by, Include: The outer part includes the bottom plate (10) and the outer sleeve (1) and the inner sleeve (2) connected to the bottom plate (10), the inner sleeve (2) is located inside the outer sleeve (1), and the cell culture frame (3) is arranged inside the inner sleeve (2); The inner part includes a coil fixing cylinder (4) and a coil (5) wound on the coil fixing cylinder (4), and the coil fixing cylinder (4) is sleeved outside the inner sleeve (2); Wherein, the inner diameter of the outer sleeve (1) is greater than the outer diameter of the inner sleeve (2), a condensation cooling cabin is formed between the outer sleeve (1) and the inner sleeve (2), and the condensation cooling cabin is used for injecting cooling substances.

2. The cell culture magnetic field generating device according to claim 1, wherein The outer sleeve (1) is provided with a water outlet (9) on the side close to the bottom plate (10).

3. The cell culture magnetic field generating device of claim 1, wherein The outer sleeve (1) is provided with a wire inlet (7) and a wire outlet (8) close to both ends, respectively, and the wire inlet (7) and the wire outlet (8) are provided with sealing plugs.

4. The cell culture magnetic field generating device according to claim 3, wherein The cell culture frame (3) is located in the middle of the inner sleeve (2) and has a hollow shape of a rice-shaped, a cross-shaped or a cross-shaped structure.

5. The cell culture magnetic field generating apparatus of claim 1, wherein The inner diameter of the inner sleeve (2) is 200-300mm.

6. The cell culture magnetic field generating device of claim 5, wherein The coil fixing cylinder (4) is detachably connected to the outer side of the inner sleeve (2), and the inner diameter of the coil fixing cylinder (4) is greater than the outer diameter of the inner sleeve (2).

7. The cell culture magnetic field generating apparatus of claim 1, wherein The inner part further includes: The limiting ring (6) is movably sleeved on the end of the coil fixing cylinder (4) away from the bottom plate (10).

8. The cell culture magnetic field generating apparatus of claim 1, wherein The outer surface of the coil (5) is covered with a protective layer.

9. The cell culture magnetic field generating apparatus of claim 1, wherein The bottom plate (10), the outer sleeve (1), the inner sleeve (2) and the cell culture frame (3) are integrally formed.

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