Semi-automatic cell counting and resuspending planking integrated equipment

Abstract

The utility model provides semi-automatic cell counting and resuspending planking integrated equipment which comprises a counting instrument, a bottom plate is arranged at the lower end of the counting instrument, a cushion groove is formed in the inner end of the bottom plate, a culture dish is arranged at the inner end of the cushion groove, a hopper cover is arranged at the upper end of the culture dish, a lower inclined plate is arranged between the hopper cover and the culture dish, and an upper inclined block is arranged at the inner end of the hopper cover. A linkage spring is arranged between the linkage ring and the upper end of the hopper cover; an opening groove is formed in the upper end of the linkage test tube; a positioning groove and a positioning block are arranged at the inner end of the opening groove; a piston rod is arranged at the inner end of the linkage test tube; a linkage seat is arranged at the upper end of the telescopic rod, a linkage motor is arranged at the upper end of the linkage seat, and the culture dish is embedded in the cushion groove. The problem that cell counting is incomplete easily due to the fact that cell sap cannot be conveniently and evenly distributed into a culture dish through the design of cell experiment equipment is solved.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell experiment equipment technical field, specifically, relate to semi -automatic cell counting and resuspension plating integrated equipment. BACKGROUND

[0002] The application of semi-automatic cell counting and resuspension plating integrated equipment in cell experiment marks an important step towards more efficient and accurate cell culture technology. This equipment combines advanced counting technology and automation, and is particularly suitable for scenarios that require large-scale repetitive experiments or high-throughput screening, such as drug development, genetic engineering, and disease model construction. In the process of new drug development, researchers often need to test thousands of compounds on specific cells. This equipment can quickly complete large-scale cell counting and plating, speed up data collection, and shorten the drug discovery cycle. After using CRISPR-Cas9 and other gene editing technologies, it is necessary to evaluate the survival rate and expression changes of genetically modified cells under different conditions. Automated equipment can effectively monitor and record the number and health status of cells, facilitating accurate analysis. In the field of cancer treatment, personalized immunotherapy solutions such as CAR-T cell therapy rely on the large-scale expansion and quality control of patients' own T cells. This equipment can ensure the uniformity and activity of cells, improving the accuracy of treatment efficacy prediction. Building cell-level disease models is crucial for understanding pathological mechanisms. Through automated cell resuspension and plating, a variety of model systems can be established in a shorter time, such as research on neurodegenerative diseases, cardiovascular diseases, and infectious diseases. Stem cell research involves complex culture condition adjustment and differentiation induction. This equipment can fine-tune cell environment parameters, promote stem cells to maintain an undifferentiated state or undergo directional differentiation, and promote the development of tissue repair and regenerative medicine.

[0003] In the prior art, during the use of cell experiment equipment, the cell liquid cannot be evenly distributed in the culture dish, which may cause incomplete cell counting. Therefore, we improve it and propose semi-automatic cell counting and resuspension plating integrated equipment. UTILITY MODEL CONTENT

[0004] The purpose of the utility model is to solve the problem that the current cell experiment equipment design cannot conveniently distribute cell liquid evenly in the culture dish, which may cause incomplete cell counting.

[0005] To achieve the above utility model purpose, the utility model provides the following technical scheme:

[0006] Semi-automatic cell counting and resuspension plating integrated equipment to improve the above problems.

[0007] The application is as follows:

[0008] The utility model provides a semi -automatic cell counting and resuspension plating integrated equipment, including counter, the lower end of counter is equipped with the bottom plate, the inner end of bottom plate is equipped with the cushion groove, the inner end of cushion groove is equipped with the petri dish, the upper end of petri dish is equipped with the hopper cover, the hopper cover and petri dish between are equipped with the lower inclined plate, the inner end of hopper cover is equipped with the upper inclined block, the upper end of upper inclined block is equipped with the linkage test tube, the outer end of linkage test tube is equipped with the linkage ring, the upper end of linkage ring and hopper cover between is equipped with the linkage spring, the upper end of linkage test tube is equipped with the open slot, the inner end of open slot is equipped with the locating groove and the locating block, the inner end of linkage test tube is equipped with the piston rod, the upper end of linkage test tube is equipped with the telescopic rod, the upper end of telescopic rod is equipped with the linkage seat, the upper end of linkage seat is equipped with the linkage motor.

[0009] As the preferred technical scheme of the present application, the petri dish is embeddedly installed in the cushion groove, the petri dish and the hopper cover are connected through the lower inclined plate, the upper end of the lower inclined plate is fixedly connected with the lower end of the hopper cover, and the outer end of the linkage test tube is fixedly connected with the linkage ring.

[0010] As the preferred technical scheme of the present application, the lower end of the linkage ring is movably connected with the outer surface of the upper end of the linkage spring, the lower end of the linkage spring is fixedly connected with the outer surface of the upper end of the hopper cover, and the inner end of the hopper cover is in contact with the upper inclined block.

[0011] As the preferred technical scheme of the present application, the inner end of the upper inclined block is annularly arranged with the distribution groove.

[0012] As the preferred technical scheme of the present application, the upper end of the upper inclined block is fixedly connected with the linkage test tube, the inner end of the linkage test tube is slidably connected with the piston rod, the outer end of the piston rod is movably connected with the telescopic rod, and the upper and lower ends of the linkage test tube are separated by the open slot.

[0013] As the preferred technical scheme of the present application, the upper end of the open slot is fixedly connected with the locating block, the locating groove is embedded in the inner surface of the lower end of the open slot, the locating block is connected with the locating groove through clamping, the upper end of the telescopic rod is movably connected with the linkage seat, and the upper end of the linkage seat is movably connected with the linkage motor.

[0014] Compared with the prior art, the utility model has the advantages of:

[0015] In the scheme of the present application:

[0016] Through the hopper cover, the linkage test tube and the piston rod, the cell liquid can be uniformly distributed on the petri dish through the closed structure, and the cell liquid can be uniformly distributed through the distribution groove, so that the cell liquid can uniformly fall from the inner wall of the hopper cover.

[0017] The closed design reduces the risk of cross contamination, protects the safety of the experimenters and the purity of the samples DRAWINGS

[0018] Figure 1The whole structure schematic diagram of the semi-automatic cell counting and resuspension plating integrated equipment provided in the application is shown in the figure.

[0019] Figure 2 The whole side structure schematic diagram of the semi-automatic cell counting and resuspension plating integrated equipment provided in the application is shown in the figure.

[0020] Figure 3 The counting instrument side structure right view of the semi-automatic cell counting and resuspension plating integrated equipment provided in the application is shown in the figure.

[0021] Figure 4 The counting instrument side structure right view of the semi-automatic cell counting and resuspension plating integrated equipment provided in the application is shown in the figure. Figure 2 The enlarged structure schematic diagram of A is shown in the figure.

[0022] The figure shows:

[0023] 1, counting instrument; 2, bottom plate; 3, pad groove; 4, culture dish; 5, bucket cover; 6, lower inclined plate; 7, upper inclined block; 8, linkage ring; 9, linkage test tube; 10, liquid separation groove; 11, linkage spring; 12, piston rod; 13, opening groove; 14, linkage seat; 15, positioning groove; 16, positioning block. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical scheme and advantages of the embodiments of the present application more clear, the technical scheme in the embodiments of the present application will be described clearly and completely below in conjunction with the drawings. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments.

[0025] Therefore, the following detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application, but only represents some embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application. It should be noted that the embodiments in the present application and the features and technical solutions in the embodiments can be combined with each other without conflict.

[0026] It should be noted that: similar labels and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0027] As Figures 1-4As shown, the present embodiment proposes a semi-automatic cell counting and resuspension plating integrated device, which comprises a counter 1, the lower end of the counter 1 is provided with a base plate 2, the inner end of the base plate 2 is provided with a pad groove 3, the inner end of the pad groove 3 is provided with a culture dish 4, the upper end of the culture dish 4 is provided with a hopper cover 5, the hopper cover 5 and the culture dish 4 are provided with a lower inclined plate 6, the inner end of the hopper cover 5 is provided with an upper inclined block 7, the upper end of the upper inclined block 7 is provided with a linkage test tube 9, the outer end of the linkage test tube 9 is provided with a linkage ring 8, the linkage ring 8 and the upper end of the hopper cover 5 are provided with a linkage spring 11, the upper end of the linkage test tube 9 is provided with an open slot 13, the inner end of the open slot 13 is provided with a positioning groove 15 and a positioning block 16, the inner end of the linkage test tube 9 is provided with a piston rod 12, the upper end of the linkage test tube 9 is provided with a telescopic rod, the upper end of the telescopic rod is provided with a linkage seat 14, the upper end of the linkage seat 14 is provided with a linkage motor.

[0028] The counter 1 is used for automatic identification and calculation of cell number, and provides fast and accurate data support;

[0029] The base plate 2 and the pad groove 3 provide a stable support foundation to ensure the stability of the device during operation;

[0030] In the culture dish 4 and the hopper cover 5, the culture dish 4 provides a basic environment for cell growth; the hopper cover 5 is used in cooperation with it to prevent external pollution, and at the same time helps to introduce and discharge liquid;

[0031] The lower inclined plate 6 guides the hopper cover 5 to tightly fit with the culture dish 4 to ensure smooth flow of liquid; the inner part of the upper inclined block 7 helps to realize uniform distribution of cell liquid;

[0032] The linkage test tube 9, the linkage ring 8 and the linkage spring 11 are used in combination to realize controllable transmission of cell liquid from the linkage test tube 9 to the culture dish 4, and to ensure operation precision and smoothness.

[0033] The piston rod 12 and the telescopic rod work together to control the accurate amount of cell liquid

[0034] The culture dish 4 is embedded and installed in the pad groove 3, the culture dish 4 and the hopper cover 5 are connected through the clamping connection of the lower inclined plate 6, the upper end of the lower inclined plate 6 and the lower end of the hopper cover 5 are fixedly connected, the outer end of the linkage test tube 9 and the linkage ring 8 are fixedly connected.

[0035] The lower end of the linkage ring 8 and the outer surface of the upper end of the linkage spring 11 are movably connected, the lower end of the linkage spring 11 and the outer surface of the upper end of the hopper cover 5 are fixedly connected, and the inner end of the hopper cover 5 and the upper inclined block 7 are in contact with each other.

[0036] The inner end of the upper inclined block 7 is annularly arranged with a liquid distribution groove 10.

[0037] The upper end of the upper inclined block 7 is fixedly connected with the linkage test tube 9, the inner end of the linkage test tube 9 is slidably connected with the piston rod 12, the outer end of the piston rod 12 is movably connected with the telescopic rod, and the upper and lower ends of the linkage test tube 9 are separated by the open slot 13.

[0038] The upper end of the open slot 13 is fixedly connected with the positioning block 16, the positioning slot 15 is embedded in the inner surface of the lower end of the open slot 13, the positioning block 16 is clampedly connected with the positioning slot 15, the upper end of the telescopic rod is movably connected with the linkage seat 14, and the upper end of the linkage seat 14 is movably connected with the linkage motor.

[0039] When the experiment cells need to be resuspended, plated and counted, in order to uniformly arrange the cell liquid in the culture dish 4, the lower inclined plate 6 is clamped into the hopper cover 5 at the upper end of the culture dish 4, the linkage test tube 9 at the upper end of the hopper cover 5 is movably connected with the piston rod 12 and the telescopic rod through the positioning slot 15 and the positioning block 16, the piston rod 12 and the telescopic rod are rotated outward, the upper end opening slot 13 of the linkage test tube 9 is opened, the cell liquid is injected, the opening slot 13 is closed, the corresponding positioning slot 15 and the positioning block 16 are clamped with each other, while the piston rod 12 is driven downward by the telescopic rod, the cell liquid in the linkage test tube 9 is pushed to be injected into the inner wall of the hopper cover 5 along the periphery of the upper inclined block 7 through the distribution slot 10, so that the cell liquid flows downward along the wall of the hopper cover 5 until completely enters the culture dish 4, and then the cell liquid is uniformly arranged.

[0040] The above examples are only used to illustrate the technical solutions described in the utility model and do not limit the utility model. Although the utility model has been described in detail with reference to the above embodiments, the utility model is not limited to the above specific embodiments, and any modification or equivalent replacement of the utility model is allowed. Any technical solution and improvement within the spirit and scope of the utility model is covered in the scope of the claims of the utility model.

Claims

1. Semi-automatic cell counting and resuspension plating integrated device, comprising a counting instrument (1), characterized in that, The lower end of the counter (1) is provided with a bottom plate (2), the inner end of the bottom plate (2) is provided with a pad groove (3), the inner end of the pad groove (3) is provided with a culture dish (4), the upper end of the culture dish (4) is provided with a hopper cover (5), the hopper cover (5) and the culture dish (4) are provided with a lower inclined plate (6), the inner end of the hopper cover (5) is provided with an upper inclined block (7), the upper end of the upper inclined block (7) is provided with a linkage test tube (9), the outer end of the linkage test tube (9) is provided with a linkage ring (8), the linkage ring (8) and the upper end of the hopper cover (5) are provided with a linkage spring (11), the upper end of the linkage test tube (9) is provided with an opening groove (13), the inner end of the opening groove (13) is provided with a positioning groove (15) and a positioning block (16), the inner end of the linkage test tube (9) is provided with a piston rod (12), the upper end of the linkage test tube (9) is provided with a telescopic rod, the upper end of the telescopic rod is provided with a linkage seat (14), the upper end of the linkage seat (14) is provided with a linkage motor.

2. The semi-automatic cell counting and resuspension plating integrated device according to claim 1, wherein, The culture dish (4) is embeddedly installed in the pad groove (3), the culture dish (4) and the hopper cover (5) are connected through the clamping connection of the lower inclined plate (6), the upper end of the lower inclined plate (6) and the lower end of the hopper cover (5) are fixedly connected, and the outer end of the linkage test tube (9) and the linkage ring (8) are fixedly connected.

3. The semi-automatic cell counting and resuspension plating integrated device according to claim 2, wherein, The lower end of the linkage ring (8) and the outer surface of the upper end of the linkage spring (11) are movably connected, the lower end of the linkage spring (11) and the outer surface of the upper end of the hopper cover (5) are fixedly connected, and the inner end of the hopper cover (5) and the upper inclined block (7) are in contact with each other.

4. The semi-automatic cell counting and resuspension plating integrated device according to claim 3, wherein, The inner end of the upper inclined block (7) is annularly arranged with a separation groove (10).

5. The semi-automatic cell counting and resuspension plating integrated device according to claim 4, wherein, The upper end of the upper inclined block (7) and the linkage test tube (9) are fixedly connected, the inner end of the linkage test tube (9) and the piston rod (12) are slidably connected, the outer end of the piston rod (12) and the telescopic rod are movably connected, and the upper and lower ends of the linkage test tube (9) are separated by the opening groove (13).

6. The semi-automatic cell counting and resuspension plating integrated device according to claim 5, wherein, The upper end of the opening groove (13) and the positioning block (16) are fixedly connected, the positioning groove (15) is embedded in the inner surface of the lower end of the opening groove (13), the positioning block (16) and the positioning groove (15) are clampedly connected, the upper end of the telescopic rod and the linkage seat (14) are movably connected, and the upper end of the linkage seat (14) and the linkage motor are movably connected.

Images