Automatic counting device for cell colonies under microscope

Abstract

The utility model provides an automatic counting device for cell colonies under a microscope, which comprises a display mirror bracket and a counting camera barrel, an observation mirror is arranged at the lower end of the counting camera barrel, an induction barrel is arranged at the outer end of the counting camera barrel, an induction plate is arranged at the outer end of the induction barrel, the induction plate is attached to the inner surface of the display mirror bracket, and the outer end of the display mirror bracket is connected with a data transmission wire harness. A movable plate and a positioning plate are arranged at the lower end of the display mirror frame, an opening is formed in the outer end of the movable plate, sealing rings are arranged at the inner ends of the movable plate and the positioning plate, a linkage shaft is arranged at the inner end of the opening, a linkage bevel gear is connected to one end of the linkage shaft, a rotating bevel gear is meshed with the outer end of the linkage bevel gear, and a hexagonal rod is arranged at the outer end of the rotating bevel gear; a hexagonal cylinder is arranged at the outer end of the hexagonal rod, and an elastic cylinder is arranged at the inner end of the hexagonal cylinder. The problems that the number of cell colonies cannot be monitored according to time intervals in the design of cell test counting, and an automatic counting device cannot adapt to microscopes of different models are solved.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell test counting technical field, and specifically, relate to automatic counting device of cell colony under microscope. BACKGROUND

[0002] In traditional cell research, the counting of cell colonies mostly relies on manual or semi-automatic microscope platforms, which obtain relevant data by manually observing, marking and counting cell colonies on culture dishes. However, this method is not only time-consuming and laborious, but also susceptible to subjective factors, resulting in low data accuracy, especially when facing a large number of samples and complex structures. In order to overcome these limitations, researchers have developed a new type of automatic counting device for cell colonies under microscope, aiming to improve experimental efficiency and data accuracy.

[0003] In the prior art, the number of cell colonies cannot be monitored at time intervals during the use of cell test counting, and the automatic counting device cannot be adapted to different types of microscopes. Therefore, we improve it and propose an automatic counting device for cell colonies under microscope. UTILITY MODEL CONTENT

[0004] The utility model aims at: according to the design of the present cell test counting, the number of cell colonies cannot be monitored at time intervals, and the automatic counting device cannot be adapted to different types of microscopes.

[0005] In order to achieve the above utility model purposes, the utility model provides the following technical scheme:

[0006] The automatic counting device for cell colonies under microscope is used to improve the above problems.

[0007] The application is as follows:

[0008] The automatic counting device for cell colonies under microscope comprises a display mirror holder and a counting camera tube, the lower end of the counting camera tube is provided with an observation mirror, the outer end of the counting camera tube is provided with a sensing tube, the outer end of the sensing tube is provided with a sensing plate, the sensing plate is attached to the inner surface of the display mirror holder, the outer end of the display mirror holder is connected with a data transmission line bundle, the lower end of the display mirror holder is provided with a movable plate and a positioning plate, the outer end of the movable plate is provided with an opening, the inner ends of the movable plate and the positioning plate are both provided with sealing rings, the inner end of the opening is provided with a linkage shaft, one end of the linkage shaft is connected with a linkage bevel gear, the outer end of the linkage bevel gear is engaged with a rotating bevel gear, the outer end of the rotating bevel gear is provided with a hexagonal rod, the outer end of the hexagonal rod is provided with a hexagonal tube, the inner end of the hexagonal tube is provided with an elastic tube, the outer end of the hexagonal tube is provided with a positioning block, the outer end of the positioning block is provided with a positioning groove, and an inner ring groove is arranged between the linkage bevel gear and the rotating bevel gear.

[0009] As the preferred technical scheme of the present application, the inductive cylinder and the inductive plate are electrically connected, the inductive plate is fixed at the inner end of the display mirror frame, the number of the inductive plate is set to six groups, the six groups of inductive plates are arranged in a ring array along the inner end of the display mirror frame, the inductive cylinder is wrapped around the outer end of the counting camera cylinder, and the outer end of the six groups of inductive plates is electrically connected with the data transmission wire harness.

[0010] As the preferred technical scheme of the present application, the positioning plate is fixed on the outer surface of the lower end of the display mirror frame, the number of the positioning plate is set to two groups, the two groups of positioning plates are arranged through the opening, the inner end of the opening is movably connected with the linkage shaft, and the linkage shaft is fixedly connected with the positioning plate.

[0011] As the preferred technical scheme of the present application, the two ends of the linkage shaft are movably connected with the two groups of positioning plates, the linkage bevel gear is fixed on the outer end of the linkage shaft at one end, the outer end of the linkage bevel gear is meshingly connected with the rotating bevel gear, and the inner ring groove is embedded in the inner end of the linkage bevel gear.

[0012] As the preferred technical scheme of the present application, the inner end of the inner ring groove is movably connected with the ring plate, the ring plate is fixedly connected with the rotating bevel gear, the outer end of the rotating bevel gear is fixedly connected with the hexagonal rod, the hexagonal rod is slidably connected with the hexagonal cylinder, and the inner end of the hexagonal cylinder is movably connected with the elastic cylinder.

[0013] As the preferred technical scheme of the present application, the inner end of the elastic cylinder is provided with a linkage spring, the two ends of the linkage spring are fixedly connected with the elastic cylinder and the hexagonal rod respectively, the tail end of the hexagonal cylinder is fixedly connected with the positioning block, the positioning block slides forward and backward along the positioning groove, and the positioning groove is embedded in the outer surface of the display mirror frame.

[0014] Compared with the prior art, the present application has the following beneficial effects:

[0015] In the scheme of the present application:

[0016] The inductive plate and the inductive cylinder are arranged, so that the image information of the counting camera cylinder can be quickly transmitted outward through the data transmission wire harness, and the number of cell colonies can be monitored conveniently.

[0017] The angle of the movable plate at the lower end of the display mirror frame can be adjusted conveniently, so that the device can be used on different types of microscopes, and has adjustability and multi-performance adaptability. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 The overall structure diagram of the microscope cell colony automatic counting device provided by the present application is shown in the figure;

[0019] Figure 2 The side sectional structure diagram of the microscope cell colony automatic counting device provided by the present application is shown in the figure;

[0020] Figure 3 The automatic counting device for cell colony under microscope provided by the present application has the advantages of Figure 2 The front view of the display frame;

[0021] Figure 4 The schematic diagram of the opening cross-section structure of the automatic counting device for cell colony under microscope provided by the present application;

[0022] Figure 5 The schematic diagram of the enlarged structure of the automatic counting device for cell colony under microscope provided by the present application; Figure 4 The schematic diagram of the enlarged structure of the automatic counting device for cell colony under microscope provided by the present application;

[0023] Figure 6 The schematic diagram of the side cross-section structure of the movable plate of the automatic counting device for cell colony under microscope provided by the present application.

[0024] Indicated in the figure:

[0025] 1, display frame; 2, counting camera barrel; 3, observation mirror; 4, movable plate; 5, sealing ring; 6, opening; 7, linkage shaft; 8, positioning plate; 9, data transmission wire harness; 10, induction plate; 11, induction barrel; 12, linkage bevel gear; 13, rotating bevel gear; 14, inner ring groove; 15, hexagonal barrel; 16, hexagonal rod; 17, elastic barrel; 18, positioning block; 19, positioning groove. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical scheme and advantages of the embodiments of the present application more clear, the technical scheme in the embodiments of the present application will be described clearly and completely below in conjunction with the drawings. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments.

[0027] Therefore, the following detailed description of the embodiments of the present application is not intended to limit the scope of the claimed present application, but only represents some embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative efforts are within the scope of protection of the present application. It should be noted that, in the case of no conflict, the embodiments in the present application and the features and technical schemes in the embodiments can be combined with each other.

[0028] It should be noted that: similar labels and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in the subsequent drawings.

[0029] As Figures 1-4As shown, the present embodiment proposes an automatic cell colony counting device under microscope, which comprises a display frame 1 and a counting camera tube 2. The lower end of the counting camera tube 2 is provided with an observation mirror 3, and the outer end of the counting camera tube 2 is provided with a sensing tube 11. The outer end of the sensing tube 11 is provided with a sensing plate 10, which is attached to the inner surface of the display frame 1. The outer end of the display frame 1 is connected with a data transmission line bundle 9. The lower end of the display frame 1 is provided with a movable plate 4 and a positioning plate 8. The outer end of the movable plate 4 is provided with an opening 6. The inner ends of the movable plate 4 and the positioning plate 8 are both provided with a sealing ring 5. The inner end of the opening 6 is provided with a linkage shaft 7. One end of the linkage shaft 7 is connected with a linkage bevel gear 12. The outer end of the linkage bevel gear 12 is engaged with a rotating bevel gear 13. The outer end of the rotating bevel gear 13 is provided with a hexagonal rod 16. The outer end of the hexagonal rod 16 is provided with a hexagonal tube 15. The inner end of the hexagonal tube 15 is provided with a spring tube 17. The outer end of the hexagonal tube 15 is provided with a positioning block 18. The outer end of the positioning block 18 is provided with a positioning groove 19. An inner ring groove 14 is provided between the linkage bevel gear 12 and the rotating bevel gear 13.

[0030] The sensing tube 11 is electrically connected with the sensing plate 10. The sensing plate 10 is fixed at the inner end of the display frame 1. The number of sensing plates 10 is set to six groups. The six groups of sensing plates 10 are arranged in a ring array along the inner end of the display frame 1. The sensing tube 11 is wrapped around the outer end of the counting camera tube 2. The outer ends of the six groups of sensing plates 10 are electrically connected with the data transmission line bundle 9.

[0031] Data transmission mechanism: The electrical connection between the sensing tube 11 and the sensing plate 10. In our microscope design, the sensing tube 11 tightly wraps around the outer side of the counting camera tube 2, and the sensing plate 10 is installed at the outer end. The sensing plate 10 is closely attached to the inner surface of the display frame 1. Each group of sensing plates 10 is directly embedded in the display frame 1 and forms a ring array with the other five groups of sensing plates 10.

[0032] Electromagnetic signal transmission: When capturing microscopic images is needed, the information from the cell colonies is received by the sensing tube 11 and converted into electromagnetic signals. These signals are then transmitted to the sensing plate 10 located inside the display frame 1. Due to the electrical connection between the sensing plate 10 and the sensing tube 11, the electromagnetic signals can be seamlessly transmitted. This direct contact transmission method ensures efficient and accurate information transmission.

[0033] Data line bundle connection: The outer edges of the six groups of sensing plates 10 are connected with the data transmission line bundle 9, thereby establishing a path for transmitting image data from the sensing plate 10 to the external display device. When electromagnetic signals reach the sensing plate 10, they will be transmitted to the external display device through this group of line bundles for processing and display.

[0034] Cell colony quantity recording process

[0035] ① Counting camera tube 2 imaging: An observation lens 3 is installed below the microscope for detailed observation of the target sample. At the same time, the counting camera tube 2 serves as the main imaging tool, capturing clear images of cell colonies under high magnification conditions.

[0036] ② Image Acquisition and Recognition: The built-in sensor plate 10 and sensor tube 11 work together to collect specific information about cell colonies, such as size and shape parameters. This image data is then converted into electrical signals by the sensor tube 11 and sent to the sensor plate 10, and then transmitted to an external processor for analysis and processing via a data cable.

[0037] ③ Automated counting function: Based on deep learning algorithms and image recognition technology, the processor can quickly identify and calculate the number of cell colonies in the captured images. This process saves time, improves accuracy, and avoids the influence of human error on experimental results.

[0038] like Figures 5-6 As shown, in a preferred embodiment, based on the above method, the positioning plate 8 is further fixed on the lower outer surface of the display frame 1. The number of positioning plates 8 is set to two sets, and the two sets of positioning plates 8 are arranged separately through an opening 6. The inner end of the opening 6 is movably connected to the linkage shaft 7, and the linkage shaft 7 is fixedly connected to the positioning plate 8.

[0039] The two ends of the linkage shaft 7 are movably connected to the two sets of positioning plates 8 respectively. The linkage bevel gear 12 is fixed to the outer end of the linkage shaft 7 at one end. The outer end of the linkage bevel gear 12 is meshed with the rotating bevel gear 13. The inner ring groove 14 is embedded in the inner end of the linkage bevel gear 12.

[0040] The inner end of the inner ring groove 14 is movably connected to the ring plate, the ring plate is fixedly connected to the rotating bevel gear 13, the outer end of the rotating bevel gear 13 is fixedly connected to the hexagonal rod 16, the hexagonal rod 16 is slidably connected to the hexagonal cylinder 15, and the inner end of the hexagonal cylinder 15 is movably connected to the elastic cylinder 17.

[0041] The inner end of the elastic cylinder 17 is provided with a linkage spring. The two ends of the linkage spring are fixedly connected to the elastic cylinder 17 and the hexagonal rod 16 respectively. The tail end of the hexagonal cylinder 15 is fixedly connected to the positioning block 18. The positioning block 18 slides back and forth along the positioning groove 19. The positioning groove 19 is embedded in the outer surface of the display frame 1.

[0042] The display frame 1 is sleeved on the upper end of the microscope, in order to facilitate the display frame 1 to stably monitor the cell colony automatic counting device, at this time, the elastic cylinder 17 and the hexagonal cylinder 15 are pulled outward, the positioning block 18 is away from the positioning groove 19, the hexagonal cylinder 15 is rotated, the rotation of the rotating bevel gear 13 is driven through the hexagonal rod 16, and the rotation of the linkage bevel gear 12 is driven through the rotating bevel gear 13, in the rotation process of the linkage bevel gear 12, the inner ring groove 14 and the ring plate are connected with each other, so that the movable plate 4 is conveniently retracted inwards along the rotation axis, the upper end of the microscope is conveniently clamped and fixed, and the upper end is fixed through the sealing ring, so that the stability of the whole is ensured.

[0043] The above examples are only used to illustrate the present application and are not limited to the technical solutions described in the present application. Although the present application has been described in detail with reference to the above embodiments, the present application is not limited to the above specific embodiments. Therefore, any modification or equivalent replacement of the present application; and all technical solutions and improvements without departing from the spirit and scope of the present application are all included in the scope of the claims of the present application.

Claims

1. An apparatus for automatic counting of cell colonies under a microscope, comprising a display stand (1) and a counting camera tube (2), characterized in that, The lower end of the counting camera tube (2) is provided with an observation mirror (3), the outer end of the counting camera tube (2) is provided with a sensing tube (11), the outer end of the sensing tube (11) is provided with a sensing plate (10), the sensing plate (10) is attached to the inner surface of the display mirror frame (1), the outer end of the display mirror frame (1) is connected with the data transmission wire harness (9), the lower end of the display mirror frame (1) is provided with a movable plate (4) and a positioning plate (8), the outer end of the movable plate (4) is provided with an opening (6), the inner end of the movable plate (4) and the positioning plate (8) is provided with a sealing ring (5), the inner end of the opening (6) is provided with a linkage shaft (7), one end of the linkage shaft (7) is connected with a linkage bevel gear (12), the outer end of the linkage bevel gear (12) is engaged with a rotating bevel gear (13), the outer end of the rotating bevel gear (13) is provided with a hexagonal rod (16), the outer end of the hexagonal rod (16) is provided with a hexagonal cylinder (15), the inner end of the hexagonal cylinder (15) is provided with a elastic cylinder (17), the outer end of the hexagonal cylinder (15) is provided with a positioning block (18), the outer end of the positioning block (18) is provided with a positioning groove (19), the linkage bevel gear (12) and the rotating bevel gear (13) are provided with an inner ring groove (14).

2. The apparatus according to claim 1, wherein The sensing tube (11) and the sensing plate (10) are electrically connected, the sensing plate (10) is fixed to the inner end of the display mirror frame (1), the number of the sensing plate (10) is set to six groups, the six groups of the sensing plate (10) are arranged in a ring array along the inner end of the display mirror frame (1), the sensing tube (11) is wrapped around the outer end of the counting camera tube (2), the outer end of the six groups of the sensing plate (10) is electrically connected with the data transmission wire harness (9).

3. The apparatus according to claim 2, wherein The positioning plate (8) is fixed to the lower end of the display mirror frame (1), the number of the positioning plate (8) is set to two groups, the two groups of the positioning plate (8) are arranged separately through the opening (6), the inner end of the opening (6) is movably connected with the linkage shaft (7), the linkage shaft (7) is fixedly connected with the positioning plate (8).

4. The apparatus according to claim 3, wherein The two ends of the linkage shaft (7) are movably connected with the two groups of the positioning plate (8), the linkage bevel gear (12) is fixed to the outer end of the linkage shaft (7) at one end, the outer end of the linkage bevel gear (12) is meshingly connected with the rotating bevel gear (13), the inner ring groove (14) is embedded in the inner end of the linkage bevel gear (12).

5. The apparatus according to claim 4, wherein The inner end of the inner ring groove (14) is movably connected with the ring plate, the ring plate is fixedly connected with the rotating bevel gear (13), the outer end of the rotating bevel gear (13) is fixedly connected with the hexagonal rod (16), the hexagonal rod (16) is slidingly connected with the hexagonal cylinder (15), the inner end of the hexagonal cylinder (15) is movably connected with the elastic cylinder (17).

6. The apparatus according to claim 5, wherein The inner end of the elastic cylinder (17) is provided with a linkage spring, both ends of the linkage spring are fixedly connected with the elastic cylinder (17) and the hexagonal rod (16) respectively, the tail end of the hexagonal cylinder (15) is fixedly connected with the positioning block (18), the positioning block (18) slides back and forth along the positioning groove (19), and the positioning groove (19) is embedded in the outer surface of the display mirror frame (1).

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